CN108378261A - Oyster protein sports drink and preparation method thereof - Google Patents
Oyster protein sports drink and preparation method thereof Download PDFInfo
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- CN108378261A CN108378261A CN201810129432.0A CN201810129432A CN108378261A CN 108378261 A CN108378261 A CN 108378261A CN 201810129432 A CN201810129432 A CN 201810129432A CN 108378261 A CN108378261 A CN 108378261A
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 60
- 235000011496 sports drink Nutrition 0.000 title claims abstract description 47
- 238000002360 preparation method Methods 0.000 title claims abstract description 40
- 238000005238 degreasing Methods 0.000 claims abstract description 33
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- 241000255789 Bombyx mori Species 0.000 claims description 5
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
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- 150000001408 amides Chemical class 0.000 claims 1
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- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 4
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- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 4
- TUKVPUFCHWKAIG-UHFFFAOYSA-N 2-benzoyl-2,3-dihydroxy-3-methoxybutanedioic acid Chemical compound COC(O)(C(O)=O)C(O)(C(O)=O)C(=O)C1=CC=CC=C1 TUKVPUFCHWKAIG-UHFFFAOYSA-N 0.000 description 3
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241000548230 Crassostrea angulata Species 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
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- 150000001299 aldehydes Chemical class 0.000 description 2
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- 235000015170 shellfish Nutrition 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 229960003080 taurine Drugs 0.000 description 2
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 2
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 2
- 241000237519 Bivalvia Species 0.000 description 1
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
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- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- 240000007175 Datura inoxia Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
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- 206010051820 Sordes Diseases 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
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- 229910021529 ammonia Inorganic materials 0.000 description 1
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- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
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- 239000011593 sulfur Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Non-Alcoholic Beverages (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of Oyster Protein sports drink and preparation method thereof, which includes following component and its parts by weight：35 parts of Oyster Protein, 0.5 1.5 parts of trehalose, 0.3 0.5 parts of muscle glycogen, 0.3 1 parts of amino acid, 0.01 0.03 parts of peach gum, 36 parts of glucose, 25 parts of fruit vegetable powder, 0.03 0.05 parts of sodium, 0.008 0.012 parts of potassium, 0.08 0.01 parts of vitamin, 80 100 parts of water.It has the beneficial effect that：The mutual gain of ingredient of sports drink of the present invention, can effectively supplement the energy and nutriment consumed during the motion, have anti-oxidant, strengthen immunity, antiviral isoreactivity；The preparation method simple possible of the sports drink, the degreasing rate of Oyster Protein is high in preparation process, yield is high, without fishy smell, can improve the thermal denaturation temperature of albumen.
Description
Technical field
The present invention relates to natural products deep process technology fields, more particularly to Oyster Protein sports drink and its preparation side
Method.
Background technology
Oyster（oyster）It is to belong to Mollusca Bivalvia pearl shell purpose Qu Li sections and Ostreidae（Ostreidae,
True oyster）Or Yan Ha sections（Aviculidae, pearl oyster）Bivalve also known as oyster, oyster, oyster be yellow, oyster clam
Or male clam.Oyster is the first big cultivated shellfish in the world, worldwide it has been found that oyster kind have 100 kinds or so, it is male
Oyster is also China important marine shellfish, has more than 20 kinds in the oyster of China coast distribution, mainly has Crassostrea rivularis, Dalian Bay male
Oyster, Pacific oyster（Long oyster）（Crassostrea gigas）And close squama oyster.China is oyster culture big country, oyster money
Source electrode horn of plenty.In recent years, growth trend is presented in China oyster yield, however relies solely on conventional machining techniques production oyster production
Oneself cannot be satisfied the social development need to oyster industry now to product, and it is even more impossible to meet consumers in general to high-quality oyster product
Demand.Therefore using oyster as raw material, produced using oyster functional sexual health of the advanced machining technology production rich in various active substance
Product or drug have vast potential for future development, oneself becomes the hot spot studied both at home and abroad.Study oyster intensive processing and comprehensive utilization
Technology can not only promote the sound development of oyster secondary industry, while also can be the hair of China's modern times aquatic products intensive processing industry
Exhibition provides certain theoretical foundation, can also be the economic benefit that enterprise creates bigger.
The shell and software of oyster all have higher utility value, make full use of oyster software and oyster shell development function
Oyster product has important social value and economic value.Protein content is up to 50%, and ammonia in oyster software dry matter
Base acid composition is perfect, is good protein.It is worth pointing out that containing abundant taurine in oyster.Taurine is one
Kind sulfur-containing amino acid has the effects that eliminating inflammation and expelling toxin, hepatic cholagogic, reducing blood lipid, promotes child's brain development and Anshen Bunao.
Foreign countries, the oyster curative effect product developed as raw material using oyster software, food additives, flavouring have listed one after another.Wherein, Japan exists
The development level highest in the field, the kind of the food and curative effect product that are produced using oyster software extract as raw material up to more than 50 kinds,
Development and production have gone out the food of capsule-type, tablet form and liquid-type, curative effect product, and product form has single component product, formula production
Product, additive-based product, annual value of production is at 20,000,000,000 yen or more.And it is less to the exploitation of oyster concerned drink, especially to oyster
Protein beverage, Oyster Protein are suitable for the nutritional need of various crowds, are particularly suitable for the physilogical characteristics of movement or physical crowd,
Can be body keep the skin wet, electrolyte and energy, the beverage that can be rapidly absorbed.
The prior art such as Authorization Notice No. is the Chinese invention patent of CN102090692B, and it is anti-to disclose a seed oyster polypeptide
Tired beverage and preparation method thereof through homogenate, enzymolysis, deodorant, de- bitter, decoloration, is filtered, concentration using oyster meat as raw material
Prepare oyster polypeptide liquid；Using antifatigue plant as raw material, antifatigue plant extraction liquid is obtained using conventional traditional Chinese medicine liquid extraction method；
Weigh by weight percentage oyster polypeptide liquid 1~15%, composite sweetener 5~15%, antifatigue plant extraction liquid 5~30%,
Antifatigue confactor 0.01~0.05% and water are deployed into 100% mixed liquor, through filling and sterilization, cooling to get finished product.
Above-mentioned beverage is full of nutrition, clear, it is sour-sweet it is moderate, color is natural, peculiar natural plants flavor, good mouthfeel, taste are good, and
With significant anti-fatigue effect.Preparation method rational technology, making is simple, strong operability, is suitable for large-scale production.But
It is that fishy smell substance cannot completely remove in the preparation method.
Invention content
One of the objects of the present invention is to provide a kind of mutual gain of ingredient, it can effectively supplement and consume during the motion
Energy and nutriment, the Oyster Protein sports drink with anti-oxidant, strengthen immunity, antiviral isoreactivity.
The second object of the present invention is to provide a kind of preparation method simple possible, the degreasing of Oyster Protein in preparation process
Rate is high, yield is high, without fishy smell, can improve the thermal denaturation temperature of albumen, sports drink obtained contains various active substance and raw meat
Taste is smaller, the preparation method of the good Oyster Protein sports drink of sensory evaluation.
The present invention in view of the above technology in the problem of mentioning, the technical solution taken is：
Oyster Protein sports drink comprising following component and its parts by weight：3-5 parts of Oyster Protein, 0.5-1.5 parts of trehalose,
0.3-0.5 parts of muscle glycogen, 0.3-1 parts of amino acid, 0.01-0.03 parts of peach gum, 3-6 parts of glucose, 2-5 parts of fruit vegetable powder, sodium 0.03-
0.05 part, 0.008-0.012 parts of potassium, 0.008-0.01 parts of vitamin, 80-100 parts of water.Each ingredient is mutual in above-mentioned sports drink
Gain, it is in good taste, full of nutrition, the energy and nutriment consumed during the motion can be effectively supplemented, water is supplemented for body
Point, electrolyte and energy, quickly regain one's strength of body；The sports drink has anti-oxidant, strengthen immunity, antiviral isoreactivity, energy
The a large amount of lipid peroxide generated in motion process are effectively eliminated, maintain the stabilization of organismic internal environment, the generation of delay fatigue,
Also have the function of quickly quenching one's thirst and relatively remove lactic acid by force, to effectively eliminate fatigue.
The preparation method of Oyster Protein sports drink, including pretreatment, enzymolysis, deodorant, solid beverage preparation are specific to walk
Suddenly it is：
Pretreatment：It is 1 that solid-liquid ratio will be pressed in oyster fresh meat:3-5（g/mL）Distilled water is added, is 8000-12000r/ in rotating speed
Then homogenate 1-3min under conditions of min is 1 by volume:Degreasing agent is added into homogenate by 6-9, the degreasing at 35-45 DEG C
20-30min is filtered after cooling, and filter residue is degreasing oyster, is then dried at 50-70 DEG C, spare, which has
Degreasing rate height, solvent noresidue, it is nontoxic nonflammable, protein is indeformable the advantages that, while degreasing agent be easy recycling, can reduce
Degreasing cost；
Enzymolysis：It is 1 by solid-liquid ratio:20-30（g/mL）The phosphate buffer that pH is 7.5-8.5 is added into degreasing oyster, then adds
The neutral proteinase for entering degreasing silkworm chrysalis weight 2-4%, 50-60min is digested at 45-55 DEG C, temperature is then risen to 90-100
DEG C, heat preservation 10-20min carries out enzyme deactivation, is centrifuged in the centrifuge that temperature is 1-5 DEG C, rotating speed is 8000-10000rpm after cooling
8-10min, supernatant are Oyster Protein liquid, and enzymatic hydrolysis extracts albumen safety higher, positioned in a mild condition
Hydrolysis, hydrolytic process is also easier to control, and can retain its nutritional ingredient, and the degradation for obtaining part peptide chain can also generate
A variety of Functional Polypeptides with physiological activity, it is easier to be absorbed by organisms utilization；
Deodorant：The active dry yeast of 0.5-1% is added into Oyster Protein liquid, 20-40min is cultivated at 25-35 DEG C, then again
The activated carbon of 1-2% is added, the stir process 20-40min at 35-45 DEG C, filtering, drying is to get Oyster Protein, the dry ferment of activity
Female loose structure has suction-operated, while active dry yeast enzyme containing there are many to stench substance, converts stench substance to nothing
Stench substance, the macromolecular substances such as substance such as aldehyde, ketone of smelling as of rotten fish are by cell accumulation, and activated carbon has porous expansion cavity knot
Structure, can effectively adsorb fishy smell small-molecule substance, and the combination of steps active dry yeast and activated carbon remove enzymolysis liquid
Fishy smell, activated carbon while adsorbing fishy smell also removal activity dry ferment fermentation after remaining taste, with reach more preferably remove raw meat imitate
Fruit, and the color and luster of enzymolysis liquid can be purified；
It is prepared by sports drink：By formula measure Oyster Protein, trehalose, muscle glycogen, amino acid, peach gum, glucose, fruit vegetable powder,
Sodium, potassium, vitamin, water are uniformly mixed, homogeneous under conditions of homogenization pressure is 20-30MPa, temperature is 70-80 DEG C, then
Homogeneous under conditions of homogenization pressure is 30-40MPa, temperature is 70-80 DEG C, it is filling, it sterilizes to get sports drink.The movement
The preparation method simple possible of beverage, is easy to industrialized production, in preparation process the yield of Oyster Protein it is high, without fishy smell, be made
Sports drink stable system, containing various active substance and fishy smell is smaller, sensory evaluation is good, be easy to be esthetically acceptable to the consumers.
Preferably, the pre-treatment step mixture that degreasing agent is n-hexane, creatine monohydrate and asparagine, heavy
Amount is than being 100:1.2-1.7:The degreasing effect of 0.22-0.27, the degreasing agent are good, and the grease in oyster can be made rapidly to outside
Diffusion into the surface, within a short period of time in Fast Stripping to degreasing agent, degreasing efficiency is high, need not repeat degreasing and can reach mesh
, and the unsaturated fatty acid that the addition of creatine monohydrate and asparagine can retain in oyster homogenate will not be removed, and be improved
The nutritive value of Oyster Protein, and the thermal denaturation temperature of albumen can be improved, prevent beverage deform in later stage sterilization process and
Floccule or rotten is generated, the quality of sports drink is improved.
Preferably, deodorant step is microwave-modified activated-carbon with activated carbon, preparation method is：Activated carbon is placed in dense
It is boiled in the phosphoric acid solution that degree is 4-6%, and keeps 30-60min, rinsed repeatedly up to neutral, then with deionized water after filtering
20-30h is dried in 100-120 DEG C of baking oven, then by activated carbon under conditions of power is 500-700 microwave radiation 3-
For 7min to get microwave-modified activated-carbon, above-mentioned phosphoric acid solution contains 0.02-0.03mM di-p-methoxies benzoyltartaric acid, and
L- di-p-methoxy benzoyltartaric acid and D- di-p-methoxies benzoyltartarics acid in di-p-methoxy benzoyltartaric acid
Ratio is 1:0.03-0.05, the phosphoric acid solution can not only carry out activated carbon load hetero atom and be modified, and it is hydrophobic that activated carbon can be changed
Property, dispersibility of the activated carbon in enzymolysis liquid is improved, and acidic functionality can be introduced into activated carbon surface, and then improve
The quantity of surface acidity functional group improves the adsorption effect and the rate of adsorption of activated carbon, while L- di-p-methoxy benzoyl wine
The rational proportion of stone acid and D- di-p-methoxies benzoyltartaric acid can be to avoid the hetero atoms such as oxygen, hydrogen in activated carbon and fragrance
Key between chip breaks to form the fragrant chip of bigger, and then activated carbon is avoided to be crystallized and be graphitized in microwave treatment, protects
Demonstrate,prove that active absorption property is unaffected, the final deodorization effect for improving activated carbon, the final deodorization effect for improving activated carbon, and
Microwave radiation can occlude activated carbon half and the aperture of occlusion is opened, open new bore, improve aperture ratio, improve the bumps on surface
Degree, while the basic functionality of activated carbon surface can be increased, adsorption effect and the rate of adsorption are improved, so the microwave modification is active
The deodorant of charcoal and purification luster effect are good.
Compared with the prior art, the advantages of the present invention are as follows：1）Each mutual gain of ingredient, mouthfeel in solid beverage of the present invention
It is good, full of nutrition, the energy and nutriment consumed during the motion can be effectively supplemented, is kept the skin wet for body, electrolyte
And energy, quickly regain one's strength of body；2）The sports drink has anti-oxidant, strengthen immunity, antiviral isoreactivity, can effectively eliminate
The a large amount of lipid peroxide generated in motion process maintain the stabilization of organismic internal environment, the generation of delay fatigue also to have fast
Speed quenches one's thirst and relatively removes the effect of lactic acid by force, to effectively eliminate fatigue；3）The preparation method of solid beverage of the present invention simply may be used
Row, is easy to industrialized production, and the degreasing rate of Oyster Protein is high in preparation process, yield is high, without fishy smell, can improve the thermal change of albumen
Warm-natured degree, sports drink obtained contains various active substance and fishy smell is smaller, sensory evaluation is good, is easy to be connect by consumer
By.
Specific implementation mode
The present invention program is described further below by embodiment：
Embodiment 1：
Oyster Protein sports drink comprising following component and its parts by weight：5 parts of Oyster Protein, 0.5 part of trehalose, muscle glycogen
0.5 part, 0.3 part of amino acid, 0.03 part of peach gum, 3 parts of glucose, 5 parts of fruit vegetable powder, 0.03 part of sodium, 0.012 part of potassium, vitamin
0.008 part, 100 parts of water.Each mutual gain of ingredient, in good taste, full of nutrition in above-mentioned sports drink, can effectively replenish movement
The energy and nutriment consumed in the process keeps the skin wet for body, electrolyte and energy, quickly regains one's strength of body；The movement is drunk
Material can effectively eliminate a large amount of lipid peroxidations generated in motion process with anti-oxidant, strengthen immunity, antiviral isoreactivity
Object maintains the stabilization of organismic internal environment, the generation of delay fatigue also to have the function of quickly quenching one's thirst and relatively remove lactic acid by force, from
And effectively eliminate fatigue.
The preparation method of Oyster Protein sports drink, including pretreatment, enzymolysis, deodorant, solid beverage preparation are specific to walk
Suddenly it is：
1）Pretreatment：It is 1 that solid-liquid ratio will be pressed in oyster fresh meat:3（g/mL）Distilled water is added, in the item that rotating speed is 12000r/min
Then homogenate 1min under part is 1 by volume:9 degreasing agent is added into homogenate, the degreasing 30min at 35 DEG C, is taken out after cooling
Filter, filter residue is degreasing oyster, is then dried at 50 DEG C, spare, the defatting step have degreasing rate height, solvent noresidue,
The advantages that nontoxic nonflammable, protein is indeformable, while degreasing agent is easy recycling, can reduce degreasing cost；
2）Enzymolysis：It is 1 by solid-liquid ratio:30（g/mL）The phosphate buffer that pH is 7.5 is added into degreasing oyster, adds degreasing
The neutral proteinase of silkworm chrysalis weight 4% digests 60min at 45 DEG C, temperature is then risen to 90 DEG C, heat preservation 20min goes out
Enzyme centrifuges 8min after cooling in the centrifuge that temperature is 1 DEG C, rotating speed is 10000rpm, and supernatant is Oyster Protein liquid, enzyme
Method hydrolysis extraction albumen safety higher, carries out positioning hydrolysis, hydrolytic process is also easier to control, and energy in a mild condition
Retain its nutritional ingredient, and the degradation for obtaining part peptide chain can also generate a variety of Functional Polypeptides with physiological activity, it is easier to
It is absorbed by organisms utilization；
3）Deodorant：1% active dry yeast is added into Oyster Protein liquid, 40min is cultivated at 25 DEG C, then adds 1%
Activated carbon, the stir process 20min at 45 DEG C, filtering, dry to get Oyster Protein, the loose structure of active dry yeast is to raw meat
Sordes matter has suction-operated, while active dry yeast contains there are many enzyme, converts without substance of smelling as of rotten fish stench substance to, substance of smelling as of rotten fish
Such as aldehyde, ketone macromolecular substances are by cell accumulation, and activated carbon has porous expansion cavity structure, can effectively adsorb raw meat
Taste small-molecule substance, and the combination of steps active dry yeast and activated carbon be come the fishy smell for removing enzymolysis liquid, activated carbon is in absorption raw meat
Remaining taste after also removal activity dry ferment ferments while taste more preferably removes raw meat effect to reach, and can purify enzymolysis liquid
Color and luster；
4）It is prepared by sports drink：Oyster Protein, trehalose, muscle glycogen, amino acid, peach gum, glucose, fruits and vegetables are measured by formula
Powder, sodium, potassium, vitamin, water are uniformly mixed, homogeneous under conditions of homogenization pressure is 30MPa, temperature is 70 DEG C, then equal
Matter pressure is 40MPa, temperature is homogeneous under conditions of 70 DEG C, filling, is sterilized to get sports drink.The preparation of the sports drink
Method simple possible, is easy to industrialized production, in preparation process the yield of Oyster Protein it is high, without fishy smell, sports drink obtained
Stable system, containing various active substance and fishy smell is smaller, sensory evaluation is good, be easy to be esthetically acceptable to the consumers.
Above-mentioned pre-treatment step is with the mixture that degreasing agent is n-hexane, creatine monohydrate and asparagine, weight ratio
It is 100:1.7:0.22, the degreasing effect of the degreasing agent is good, can so that the grease in oyster rapidly to external diffusion into the surface, compared with
In short time in Fast Stripping to degreasing agent, degreasing efficiency is high, and need not repeat degreasing can achieve the goal, and creatine monohydrate and
The unsaturated fatty acid that the addition of asparagine can retain in oyster homogenate will not be removed, and improve the nutriture value of Oyster Protein
Value, and can improve the thermal denaturation temperature of albumen, prevent beverage from deforming in later stage sterilization process and generating floccule or rotten,
Improve the quality of sports drink.
Above-mentioned deodorant step is microwave-modified activated-carbon with activated carbon, and preparation method is：Activated carbon is placed in a concentration of
It is boiled in 6% phosphoric acid solution, and keeps 30min, rinsed repeatedly up to neutral, then at 120 DEG C with deionized water after filtering
20h is dried in baking oven, then by activated carbon under conditions of power is 700 microwave radiation 3min to get microwave-modified activated-carbon,
Above-mentioned phosphoric acid solution contains 0.03mM di-p-methoxies benzoyltartaric acid, and L- bis- is right in di-p-methoxy benzoyltartaric acid
The ratio of methoxybenzoyl tartaric acid and D- di-p-methoxies benzoyltartaric acid is 1:0.03, which not only can be right
Activated carbon carries out load hetero atom and is modified, and activated carbon hydrophobicity can be changed, and improves dispersibility of the activated carbon in enzymolysis liquid, and
Acidic functionality can be introduced into activated carbon surface, and then improve the quantity of surface acidity functional group, improve the suction of activated carbon
Attached effect and the rate of adsorption, while L- di-p-methoxy benzoyltartaric acid and D- di-p-methoxies benzoyltartarics acid is reasonable
Ratio can break to form the fragrant chip of bigger to avoid the key between the hetero atoms such as oxygen, hydrogen in activated carbon and fragrant chip, into
And activated carbon is avoided to be crystallized and be graphitized in microwave treatment, ensure that active absorption property is unaffected, final improve is lived
Property charcoal deodorization effect, the final deodorization effect for improving activated carbon, and microwave radiation can occlude activated carbon half and the hole of occlusion
Diameter opening, open new bore, improve aperture ratio, improve the camber on surface, while can increase the alkaline function of activated carbon surface
Adsorption effect and the rate of adsorption improve in group, so the deodorant of the microwave-modified activated-carbon and purification luster effect are good.
Embodiment 2：
Oyster Protein sports drink comprising following component and its parts by weight：3 parts of Oyster Protein, 1.5 parts of trehalose, muscle glycogen
0.3 part, amino acid 1 part, 0.01 part of peach gum, 6 parts of glucose, 2 parts of fruit vegetable powder, 0.05 part of sodium, 0.008 part of potassium, vitamin 0.01
Part, 80 parts of water.
The preparation method of Oyster Protein sports drink, including pretreatment, enzymolysis, deodorant, solid beverage preparation are specific to walk
Suddenly it is：
1）Pretreatment：It is 1 that solid-liquid ratio will be pressed in oyster fresh meat:5（g/mL）Distilled water is added, in the item that rotating speed is 8000r/min
Then homogenate 3min under part is 1 by volume:6 degreasing agent is added into homogenate, the degreasing 20min at 45 DEG C, is taken out after cooling
Filter, filter residue is degreasing oyster, is then dried at 70 DEG C, spare；
2）Enzymolysis：It is 1 by solid-liquid ratio:20（g/mL）The phosphate buffer that pH is 8.5 is added into degreasing oyster, adds degreasing
The neutral proteinase of silkworm chrysalis weight 2% digests 50min at 55 DEG C, temperature is then risen to 100 DEG C, heat preservation 10min goes out
Enzyme centrifuges 10min after cooling in the centrifuge that temperature is 5 DEG C, rotating speed is 8000rpm, and supernatant is Oyster Protein liquid；
3）Deodorant：0.5% active dry yeast is added into Oyster Protein liquid, 20min is cultivated at 35 DEG C, then adds 2%
Activated carbon, the stir process 40min at 35 DEG C, filtering is dry to get Oyster Protein；
4）It is prepared by sports drink：Oyster Protein, trehalose, muscle glycogen, amino acid, peach gum, glucose, fruits and vegetables are measured by formula
Powder, sodium, potassium, vitamin, water are uniformly mixed, homogeneous under conditions of homogenization pressure is 20MPa, temperature is 80 DEG C, then equal
Matter pressure is 30MPa, temperature is homogeneous under conditions of 80 DEG C, filling, is sterilized to get sports drink.
Above-mentioned pre-treatment step is with the mixture that degreasing agent is n-hexane, creatine monohydrate and asparagine, weight ratio
It is 100:1.2:0.22.
Above-mentioned deodorant step is microwave-modified activated-carbon with activated carbon, and preparation method is：Activated carbon is placed in a concentration of
It is boiled in 4% phosphoric acid solution, and keeps 60min, rinsed repeatedly up to neutral, then at 100 DEG C with deionized water after filtering
30h is dried in baking oven, then by activated carbon under conditions of power is 500 microwave radiation 7min to get microwave-modified activated-carbon,
Above-mentioned phosphoric acid solution contains 0.02mM di-p-methoxies benzoyltartaric acid, and L- bis- is right in di-p-methoxy benzoyltartaric acid
The ratio of methoxybenzoyl tartaric acid and D- di-p-methoxies benzoyltartaric acid is 1:0.05.
Embodiment 3：
Oyster Protein sports drink comprising following component and its parts by weight：4 parts of Oyster Protein, 1.0 parts of trehalose, muscle glycogen
0.4 part, 0.7 part of amino acid, 0.02 part of peach gum, 5 parts of glucose, 4 parts of fruit vegetable powder, 0.04 part of sodium, 0.01 part of potassium, vitamin
0.009 part, 90 parts of water.
The preparation method of Oyster Protein sports drink, including pretreatment, enzymolysis, deodorant, solid beverage preparation are specific to walk
Suddenly it is：
1）Pretreatment：It is 1 that solid-liquid ratio will be pressed in oyster fresh meat:4（g/mL）Distilled water is added, in the item that rotating speed is 10000r/min
Then homogenate 2min under part is 1 by volume:7 degreasing agent is added into homogenate, the degreasing 25min at 40 DEG C, is taken out after cooling
Filter, filter residue is degreasing oyster, is then dried at 60 DEG C, spare；
2）Enzymolysis：It is 1 by solid-liquid ratio:25（g/mL）The phosphate buffer that pH is 8.0 is added into degreasing oyster, adds degreasing
The neutral proteinase of silkworm chrysalis weight 3% digests 55min at 50 DEG C, temperature is then risen to 95 DEG C, heat preservation 15min goes out
Enzyme centrifuges 8min after cooling in the centrifuge that temperature is 4 DEG C, rotating speed is 9000rpm, and supernatant is Oyster Protein liquid；
3）Deodorant：0.8% active dry yeast is added into Oyster Protein liquid, 30min is cultivated at 30 DEG C, is then added
1.5% activated carbon, the stir process 30min at 40 DEG C, filtering are dry to get Oyster Protein；
4）It is prepared by sports drink：Oyster Protein, trehalose, muscle glycogen, amino acid, peach gum, glucose, fruits and vegetables are measured by formula
Powder, sodium, potassium, vitamin, water are uniformly mixed, homogeneous under conditions of homogenization pressure is 25MPa, temperature is 75 DEG C, then equal
Matter pressure is 35MPa, temperature is homogeneous under conditions of 75 DEG C, filling, is sterilized to get sports drink.
Above-mentioned pre-treatment step is with the mixture that degreasing agent is n-hexane, creatine monohydrate and asparagine, weight ratio
It is 100:1.5:0.25.
Above-mentioned deodorant step is microwave-modified activated-carbon with activated carbon, and preparation method is：Activated carbon is placed in a concentration of
It is boiled in 5% phosphoric acid solution, and keeps 45min, rinsed repeatedly up to neutral, then at 110 DEG C with deionized water after filtering
Dried in baking oven for 24 hours, then by activated carbon under conditions of power is 600 microwave radiation 5min to get microwave-modified activated-carbon,
Above-mentioned phosphoric acid solution contains 0.025mM di-p-methoxies benzoyltartaric acid, and L- bis- in di-p-methoxy benzoyltartaric acid
Ratio to methoxybenzoyl tartaric acid and D- di-p-methoxies benzoyltartaric acid is 1:0.04.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Claims (9)
1. Oyster Protein sports drink, it is characterised in that：The sports drink includes following component and its parts by weight：Oyster Protein
3-5 parts, 0.5-1.5 parts of trehalose, 0.3-0.5 parts of muscle glycogen, 0.3-1 parts of amino acid, 0.01-0.03 parts of peach gum, glucose 3-6
Part, 2-5 parts of fruit vegetable powder, 0.03-0.05 parts of sodium, 0.008-0.012 parts of potassium, 0.008-0.01 parts of vitamin, 80-100 parts of water.
2. the preparation method of Oyster Protein sports drink, including pretreatment, enzymolysis, deodorant, solid beverage preparation, feature exist
In：The pre-treatment step is：Distilled water will be added in oyster fresh meat, homogenate takes off then by degreasing agent is added into homogenate
Fat filters after cooling, and filter residue is degreasing oyster, and drying is spare, and the degreasing agent is n-hexane, creatine monohydrate and lucid asparagus
The mixture of amide.
3. the preparation method of Oyster Protein sports drink according to claim 2, it is characterised in that：The homogenate and degreasing
The volume ratio of agent is 1:6-9, skimming temp is 35-45 DEG C, time 20-30min.
4. the preparation method of Oyster Protein sports drink according to claim 2, it is characterised in that：In the degreasing agent just
The weight ratio of the mixture of hexane, creatine monohydrate and asparagine is 100:1.2-1.7:0.22-0.27.
5. the preparation method of Oyster Protein sports drink according to claim 2, it is characterised in that：The enzymolysis step
For：It is 1 by solid-liquid ratio:20-30（g/mL）The phosphate buffer that pH is 7.5-8.5 is added into degreasing oyster, adds degreasing
The neutral proteinase of silkworm chrysalis weight 2-4% digests 50-60min at 45-55 DEG C, and enzyme deactivation centrifuges after cooling, and supernatant is as male
Oyster protein liquid.
6. the preparation method of Oyster Protein sports drink according to claim 2, it is characterised in that：The deodorant step
For：The active dry yeast of 0.5-1% is added into Oyster Protein liquid, 20-40min is cultivated at 25-35 DEG C, then adds 1-
2% activated carbon, the stir process 20-40min at 35-45 DEG C, filtering are dry to get Oyster Protein.
7. the preparation method of Oyster Protein sports drink according to claim 6, it is characterised in that：The activated carbon is micro-
Wavefront modifier activated carbon, preparation method are：Activated carbon is placed in the phosphoric acid solution of a concentration of 4-6% and is boiled, and keeps 30-
60min is rinsed repeatedly with deionized water until neutral, drying, is then the condition of 500-700 in power by activated carbon after filtering
For lower microwave radiation 3-7min to get microwave-modified activated-carbon, the phosphoric acid solution contains 0.02-0.03mM di-p-methoxy benzene first
Acyl tartaric acid.
8. the preparation method of Oyster Protein sports drink according to claim 7, it is characterised in that：The di-p-methoxy
The ratio of L- di-p-methoxies benzoyltartaric acid and D- di-p-methoxies benzoyltartaric acid is 1 in benzoyltartaric acid:
0.03-0.05。
9. the preparation method of Oyster Protein sports drink according to claim 2, it is characterised in that：The sports drink system
It is for step：Oyster Protein, trehalose, muscle glycogen, amino acid, peach gum, glucose, fruit vegetable powder, sodium, potassium, dimension are measured by formula
Raw element, water, are uniformly mixed, homogeneous under conditions of homogenization pressure is 20-30MPa, temperature is 70-80 DEG C, then in homogeneous pressure
Homogeneous under conditions of power is 30-40MPa, temperature is 70-80 DEG C, it is filling, it sterilizes to get sports drink.
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| CN112641025A (en) * | 2020-12-01 | 2021-04-13 | 彭百生 | Protein solid brewing particles and preparation method thereof |
| CN115363151A (en) * | 2021-05-18 | 2022-11-22 | 海蒂柔化妆品科技(青岛)有限公司 | Oyster glycogen sports body-building beverage |
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Application publication date: 20180810 |