CN115363151A - Oyster glycogen sports body-building beverage - Google Patents
Oyster glycogen sports body-building beverage Download PDFInfo
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- CN115363151A CN115363151A CN202110550786.4A CN202110550786A CN115363151A CN 115363151 A CN115363151 A CN 115363151A CN 202110550786 A CN202110550786 A CN 202110550786A CN 115363151 A CN115363151 A CN 115363151A
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention relates to the technical field of drinks, in particular to an oyster glycogen sports fitness drink; the feed comprises the following raw materials in parts by weight: the feed comprises the following raw materials in parts by weight: 30-40 parts of oyster meat, 10-20 parts of alginate fiber, 8-12 parts of astaxanthin, 1-5 parts of a stabilizer, 6-10 parts of apple juice and 30-40 parts of water; the oyster glycogen in the oyster meat is extracted by taking the oyster meat, the biodegradable alginate fiber, the astaxanthin with an antioxidation function, the stabilizer and the apple juice as raw materials, the activity of the oyster glycogen is kept, the heart and blood circulation capability of a human body are improved, the liver function is enhanced, the prepared oyster glycogen sports fitness beverage also has the effects of improving the ultraviolet resistance and the blood sugar of the body, the taste of the prepared oyster glycogen sports fitness beverage is adjusted by the apple juice, the alginate fiber provides cell vitality, and the astaxanthin effectively inhibits lipid peroxidation caused by free radicals, so that the quality of the prepared oyster glycogen sports fitness beverage is effectively improved.
Description
Technical Field
The invention relates to the technical field of drinks, in particular to an oyster glycogen sports fitness drink.
Background
The sports drink is a kind of drink with the fastest market growth speed, is prepared according to the characteristics of physiological consumption during sports, can purposefully supplement nutrients lost during sports, and plays the roles of keeping and improving the sports ability and accelerating the fatigue elimination after sports.
The oyster meat contains rich glycogen, protein, amino acid, fat, trace elements, vitamins and the like, wherein the glycogen accounts for 18-39%, the protein accounts for 45-57%, and the fat accounts for 7-11%. The oyster has perfect amino acid composition, and according to the evaluation of the world food and agriculture organization, the completeness and the mass ratio of the essential amino acid in the oyster meat are superior to those of human milk and cow milk, and the oyster meat contains 20 common amino acids which are necessary for human bodies, and also contains a plurality of amino acids with important physiological values, such as beta-aminopropionic acid, gamma-aminobutyric acid, ornithine, taurine and the like. Particularly, the content of taurine is particularly rich and is obviously higher than that of other animal foods, and the content can reach 30.98 percent of the total amino acid content.
Glycogen is stored in large quantities in the liver and also in muscle, especially in some aquatic mollusks. Glycogen is also contained in fungi, bacteria and yeasts of the plant kingdom. Glycogen is chemically similar to starch and is hydrolyzed completely with acid to produce D-glucose. The structure is similar to that of collagen, and is glucan connected by a-1,4 glycosidic bonds and provided with a-1,6 glycosidic bond-connected branched sugar chains. The polymerization degree is smaller than that of the starch gum, and the average branch chain length is 12-18 glucose units.
People can process and sell sports body-building beverages by various technologies, for example, a pulse-activating sports beverage and a preparation method thereof are disclosed in the patent document with the Chinese patent application number of CN102450718B, and the sports body-building beverage prepared by the patent document has certain fatigue resistance, but lacks the effects of ultraviolet resistance and blood sugar reduction, and influences the performance and quality of the prepared sports body-building beverage.
Disclosure of Invention
Aiming at the defects in the prior art, the oyster glycogen sports fitness beverage is prepared by taking oyster meat, biodegradable alginate fibers, astaxanthin with antioxidant function, a stabilizer and apple juice as raw materials, extracting oyster glycogen from the oyster meat, and keeping the activity of the oyster glycogen to improve the heart and blood circulation capability of human beings and enhance the liver function, so that the prepared oyster glycogen sports fitness beverage also has the effects of improving the ultraviolet resistance and blood sugar reduction of organisms, and the taste of the prepared oyster glycogen sports fitness beverage is adjusted by the apple juice; so that the quality of the prepared oyster glycogen sports fitness beverage is effectively improved.
In order to achieve the purpose, the invention provides the following technical scheme:
the oyster glycogen sports fitness beverage comprises the following raw materials in parts by weight: 30-40 parts of oyster meat, 10-20 parts of alginate fiber, 8-12 parts of astaxanthin, 1-5 parts of a stabilizer, 6-10 parts of apple juice and 30-40 parts of water.
By adopting the technical scheme: the oyster glycogen sports fitness beverage is prepared by taking oyster meat, biodegradable alginate fibers, astaxanthin with an antioxidant function, a stabilizer and apple juice as raw materials, extracting oyster glycogen from the oyster meat, keeping the activity of the oyster glycogen, improving the heart and blood circulation capability of a human body and enhancing the liver function, so that the prepared oyster glycogen sports fitness beverage also has the effects of improving the ultraviolet resistance and blood sugar of the body, the astaxanthin is used for reducing lipid peroxidation through giving cell vitality through the alginate fibers, the lipid peroxidation caused by free radicals is effectively inhibited, the taste of the prepared oyster glycogen sports fitness beverage is adjusted through the apple juice, and the quality of the prepared oyster glycogen sports fitness beverage is effectively improved.
The invention is further configured to: the stabilizer is sodium hydroxymethyl cellulose.
By adopting the technical scheme: the invention takes the sodium hydroxymethyl cellulose as the stabilizer, has excellent freezing and melting stability, can improve the flavor of the product and prolong the storage time.
The preparation method of the oyster glycogen sports fitness beverage comprises the following steps:
s1, extracting oyster glycogen from oysters.
S2, performing gel filtration chromatography on the oyster glycogen.
And S3, preparing the raw materials to prepare the oyster glycogen sports fitness beverage.
By adopting the technical scheme: the invention extracts oyster glycogen from oysters and carries out oyster glycogen protogen gel filtration chromatography, so that the extracted oyster glycogen highly retains the natural active ingredients of the oysters, contains various mineral substances, trace elements and other ingredients, can prevent cardiovascular diseases, can prevent the rise of blood sugar by the oyster glycogen, and has the ability of reducing the blood sugar and the ability of ultraviolet rays.
The invention is further configured to: oyster glycogen is extracted from oysters, comprising the following steps:
s101, putting the oyster meat into a pulping machine, adding water and 30% sodium hydroxide solution into the pulping machine, heating, and cooling to room temperature.
S102, adding 95% ethanol in the step 101, uniformly mixing, filtering to obtain a precipitate, and dissolving the precipitate in water to adjust the pH value to be neutral.
S103, filtering to obtain clear liquid, putting the clear liquid into a stirrer, adding the positive resin, and stirring for 24 hours in a greenhouse.
S104, filtering again, adding 95% ethanol into the filtered clear liquid again to obtain a precipitate, hydrolyzing the obtained precipitate to remove protein, collecting the precipitate, and freeze-drying to obtain the crude extract of the glycogen of the oyster.
By adopting the technical scheme: the invention can destroy protein and nucleic acid by heating oyster meat tissue with sodium hydroxide alkali, leads glycogen to be fully dissolved out, leads the glycogen to be degraded by strong alkali, leads the dissolution of the oyster glycogen to be more ideal by positive resin treatment in the extraction process, can effectively remove protein, and leads the extracted oyster glycogen to highly reserve the natural active ingredients of oyster.
The invention is further configured to: in the step S101, heating is performed for 1h at a temperature of 100 ℃.
By adopting the technical scheme: the invention can promote the damage to the protein and nucleic acid of the oyster meat tissue by heating.
The invention is further configured to: in step S102, the PH is adjusted to be neutral using glacial acetic acid.
By adopting the technical scheme: the invention adjusts the PH value through glacial acetic acid, and provides ideal PH conditions for subsequent operations.
The invention is further configured to: in step S104, the method for hydrolyzing deproteinized protein is: and stirring and mixing the obtained precipitate and Sevage reagent, and oscillating and centrifuging to obtain the hydrolyzed deproteinized precipitate.
By adopting the technical scheme: the present invention converts protein from insoluble state to soluble state, and degrades the protein into short peptide with relatively small molecular weight and easy absorption by human body, so as to produce health beverage.
The invention is further configured to: in step S2, the oyster glycogen gel filtration chromatography comprises the following steps:
s201, taking the crude extract of the oyster glycogen, dissolving the crude extract of the oyster glycogen into eluent, and filtering.
S202, putting the oyster glycogen crude extract filtered in the S101 into agarose gel 2B, eluting by using phosphate buffer solution with pH6.8, and collecting an outflow liquid.
S203, dialyzing with distilled water, and freeze-drying to obtain the oyster glycogen.
By adopting the technical scheme: the present invention separates the separated matter components according to molecular size, screens small molecular matter, eliminates large molecular matter to the outside and maintains natural active components of oyster.
The invention is further configured to: in the step S201, filtration is performed using a 0.45um microporous membrane.
By adopting the technical scheme: the invention uses microporous membrane to filter, which is easy to purify the crude extract of oyster glycogen.
The invention is further configured to: the raw materials are blended to prepare the oyster glycogen sports fitness beverage, which comprises the following steps:
s301, putting the oyster glycogen, the alginate fibers, the astaxanthin, the stabilizer and the apple juice into a stirrer, adding water into the stirrer, stirring for 30min at the rotation speed of 300-400r/min, and filtering by using a 20-30-mesh sieve.
S302, heating the stirred mixture in a water bath for sterilization, and bottling.
By adopting the technical scheme: the invention keeps the activity of the oyster glycogen to improve the heart and blood circulation capability of people and enhance the liver function, and the prepared oyster glycogen sports fitness beverage also has the effects of improving the ultraviolet resistance of organisms and reducing blood sugar.
Advantageous effects
Compared with the known public technology, the technical scheme provided by the invention has the following beneficial effects:
the oyster glycogen in the oyster meat is extracted by taking the oyster meat, the biodegradable alginate fibers, the astaxanthin with an antioxidant function, the stabilizer and the apple juice as raw materials, the activity of the oyster glycogen is kept, the heart and blood circulation capability of a human body are improved, the liver function is enhanced, the prepared oyster glycogen sports fitness beverage also has the effects of improving the ultraviolet resistance of the body and reducing the blood sugar, the taste of the prepared oyster glycogen sports fitness beverage is adjusted through the apple juice, and the quality of the prepared oyster glycogen sports fitness beverage is effectively improved.
Drawings
Fig. 1 is a flow chart of a preparation method of the oyster glycogen sports fitness beverage.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The present invention will be further described with reference to the following examples.
Example 1
Referring to fig. 1, the oyster glycogen sports fitness beverage comprises the following raw materials in parts by weight: 30-40 parts of oyster meat, 10-20 parts of seaweed fiber, 8-12 parts of astaxanthin, 1-5 parts of stabilizer, 6-10 parts of apple juice and 30-40 parts of water.
The stabilizer is sodium hydroxymethyl cellulose.
The preparation method of the oyster glycogen sports fitness beverage comprises the following steps:
step one, oyster glycogen is extracted from oysters.
Step two, filtering and chromatography of the oyster glycogen protogen gel.
And step three, preparing raw materials to prepare the oyster glycogen sports fitness beverage.
Oyster glycogen is extracted from oysters, comprising the following steps:
101 In a beater, and water and 30% sodium hydroxide solution are added to the beater, heat treated, and then cooled to room temperature.
102 Adding 95% ethanol into the mixture obtained in the step 101), uniformly mixing, filtering the obtained precipitate, and dissolving the precipitate into water to adjust the pH value to be neutral.
103 Filtering to obtain clear liquid, placing the clear liquid in a stirrer, adding positive resin, and stirring for 24h in a greenhouse.
104 Filtering again, adding 95% ethanol into the filtered clear solution to obtain precipitate, hydrolyzing the precipitate to remove protein, collecting precipitate, and lyophilizing to obtain Concha Ostreae glycogen crude extract.
Heating at 100 deg.C for 1h.
The pH was adjusted to neutral with glacial acetic acid.
The method for hydrolyzing deproteinized protein comprises the following steps: and stirring and mixing the obtained precipitate and Sevage reagent, and oscillating and centrifuging to obtain the hydrolyzed deproteinized precipitate.
The oyster glycogen gel filtration chromatography comprises the following steps:
201 Taking crude extract of oyster glycogen, dissolving the crude extract of oyster glycogen in eluent, and filtering.
202 101) was placed in Sepharose 2B, eluted with phosphate buffer solution of pH6.8, and the effluent was collected.
203 Dialyzing with distilled water, and freeze-drying to obtain oyster glycogen.
Filtration was performed using a 0.45um microporous membrane.
The raw materials are blended to prepare the oyster glycogen sports fitness beverage, which comprises the following steps:
301 Putting the oyster glycogen, the seaweed fiber, the astaxanthin, the stabilizer and the apple juice into a stirrer, adding water into the stirrer, stirring for 30min at the rotation speed of 300r/min, and filtering by using a 20-mesh sieve.
302 Heating the stirred mixture in water bath for sterilization, and bottling.
Example 2
The oyster glycogen sports fitness beverage provided by the embodiment is approximately the same as that in the embodiment 1, and the main differences are as follows: the feed comprises the following raw materials in parts by weight: 40 parts of oyster meat, 20 parts of seaweed fiber, 12 parts of astaxanthin, 5 parts of stabilizer, 10 parts of apple juice and 40 parts of water;
in step 301, stirring for 30min at a rotation speed of 400r/min, and filtering with a 30-mesh sieve;
comparative example 1
The oyster glycogen sports fitness beverage provided by the embodiment is substantially the same as the oyster glycogen sports fitness beverage provided by the embodiment 1, and the main differences are as follows: without adding oyster meat;
comparative example 2
The oyster glycogen sports fitness beverage provided by the embodiment is approximately the same as that in the embodiment 1, and the main differences are as follows: the oyster glycogen is not separated and purified.
Performance testing
Taking the oyster glycogen sports fitness beverage prepared in the examples 1-2 and the comparative examples 1-2, and detecting the relevant performance of the prepared oyster glycogen sports fitness beverage, wherein the detection method comprises the following steps:
1. the immunity (skin injury) resistance of the oyster glycogen sports fitness beverage is detected respectively, and the obtained test results are recorded in table 1;
TABLE 1 oyster glycogen sports body-building beverage immunity-resisting effect detecting table
| Test items | Anti-ultraviolet effect |
| Example 1 | Without harm |
| Example 2 | Without harm |
| Comparative example 1 | Has injury |
| Comparative example 2 | Mild injury |
2. Respectively detecting the performance of the active ingredients (optical analysis) of the oyster glycogen sports fitness beverage, and recording the obtained test results in a table 2;
TABLE 2 table for testing the anti-immunity effect of the beverage
| Test items | Effect of optical analysis |
| Example 1 | 16.21 |
| Practice ofExample 2 | 16.24 |
| Comparative example 1 | 4.45 |
| Comparative example 2 | 5.78 |
By analyzing the relevant data in the table, the oyster glycogen sports body-building beverage prepared by the invention has good ultraviolet-proof effect and is rich in active ingredients, so that the quality of the prepared oyster glycogen sports body-building beverage is effectively improved.
2. And (3) detecting the blood sugar reducing performance:
continuously gavage the oyster glycogen sports fitness beverage for 30 days, resting for 30min after the last gavage, accurately extracting 100gm of liver, rinsing with normal saline, sucking with a paper, and according to the liver mass (mg): alkali liquor volume (uL) =1, the liver and then alkali liquor instrument are added into the test tube and boiled water bath is cooled by running water rapidly after 20min, double distilled water is added, the sample is made into 1% liver glycogen detection solution for detection, and the detection process is as follows: 1.0ml of distilled water, 0.001mg/mg of standard solution, 0.1ml of liver glycogen detection solution and 2ml of color development solution are taken, mixed uniformly and bathed in boiling water for 5min, the wavelength of the mixed solution is 620nm after cooling, the optical path of the mixed solution is 1cm, the absorbance is measured, a mouse which is not perfused with the oyster glycogen sports fitness beverage is used as a contrast, and the oyster glycogen sports fitness beverage prepared by the invention has the advantages that the liver glycogen content is lower than that of a low-dose group after continuous perfusion for 30 days.
The oyster glycogen in the oyster meat is extracted by taking the oyster meat, the biodegradable alginate fibers, the astaxanthin with an antioxidant function, the stabilizer and the apple juice as raw materials, the activity of the oyster glycogen is kept, the heart and blood circulation capability of a human body are improved, the liver function is enhanced, the prepared oyster glycogen sports fitness beverage also has the effects of improving the ultraviolet resistance of the body and reducing the blood sugar, the taste of the prepared oyster glycogen sports fitness beverage is adjusted through the apple juice, and the quality of the prepared oyster glycogen sports fitness beverage is effectively improved.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (10)
1. The oyster glycogen sports fitness beverage is characterized by comprising the following raw materials in parts by weight: 30-40 parts of oyster meat, 10-20 parts of seaweed fiber, 8-12 parts of astaxanthin, 1-5 parts of stabilizer, 6-10 parts of apple juice and 30-40 parts of water.
2. The oyster glycogen exercise fitness beverage according to claim 2, wherein the stabilizer is sodium hydroxymethyl cellulose.
3. The preparation method of oyster glycogen sports fitness beverage, characterized in that oyster glycogen sports fitness beverage according to any one of claims 1 to 2 is used, comprising the steps of:
s1, extracting oyster glycogen from oysters;
s2, filtering and chromatography of the oyster glycogen protogen gel;
and S3, preparing the raw materials to prepare the oyster glycogen sports fitness beverage.
4. The oyster glycogen sports fitness beverage according to claim 3, wherein in the step S1, oyster glycogen is extracted from oysters, comprising the steps of:
s101, putting the oyster meat into a pulping machine, adding water and 30% sodium hydroxide solution into the pulping machine, heating, and cooling to room temperature;
s102, adding 95% ethanol into the mixture obtained in the step 101, uniformly mixing, filtering to obtain a precipitate, and dissolving the precipitate into water to adjust the pH value to be neutral;
s103, filtering to obtain clear liquid, putting the clear liquid into a stirrer, adding positive resin, and stirring for 24 hours in a greenhouse;
s104, filtering again, adding 95% ethanol into the filtered clear liquid to obtain a precipitate, hydrolyzing the obtained precipitate to remove protein, collecting the precipitate, and freeze-drying to obtain the crude extract of the glycogen of the oyster.
5. The oyster glycogen sports fitness beverage according to claim 4, wherein in the step S101, heating is performed at a temperature of 100 ℃ for 1 hour.
6. The oyster glycogen sports fitness beverage according to claim 4, wherein glacial acetic acid is used to adjust pH to be neutral in the step S102.
7. The oyster glycogen exercise fitness beverage according to claim 4, wherein in the step S104, the method for hydrolyzing deproteinized protein is: and stirring and mixing the obtained precipitate and Sevage reagent, and oscillating and centrifuging to obtain the hydrolyzed deproteinized precipitate.
8. The oyster glycogen sports fitness beverage according to claim 3, wherein in the step S2, oyster glycogen gel filtration chromatography comprises the steps of:
s201, taking the crude extract of the oyster glycogen, dissolving the crude extract of the oyster glycogen into eluent, and filtering;
s202, putting the oyster glycogen crude extract filtered in the step S101 into agarose gel 2B, eluting by using phosphate buffer solution with pH6.8, and collecting effluent liquid;
s203, dialyzing with distilled water, and freeze-drying to obtain the oyster glycogen.
9. The oyster glycogen exercise fitness beverage according to claim 3, wherein in the step S201, the filtering is performed using a 0.45um microporous membrane.
10. The oyster glycogen sports fitness beverage according to claim 3, wherein the oyster glycogen sports fitness beverage prepared by blending raw materials comprises the following steps:
s301, putting the oyster glycogen, the alginate fibers, the astaxanthin, the stabilizer and the apple juice into a stirrer, adding water into the stirrer, stirring for 30min under the condition that the rotation speed is 300-400r/min, and filtering by using a 20-30-mesh sieve;
s302, heating the stirred mixture in a water bath for sterilization, and bottling.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116114816A (en) * | 2023-02-27 | 2023-05-16 | 江西奇谷电子商务有限公司 | Bamboo salt sports drink and preparation method thereof |
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