CN108378225A - A kind of preparation method for improving the feed addictive containing composite vegetables extractive of ruminant digestion absorption function - Google Patents
A kind of preparation method for improving the feed addictive containing composite vegetables extractive of ruminant digestion absorption function Download PDFInfo
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- CN108378225A CN108378225A CN201810139857.XA CN201810139857A CN108378225A CN 108378225 A CN108378225 A CN 108378225A CN 201810139857 A CN201810139857 A CN 201810139857A CN 108378225 A CN108378225 A CN 108378225A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- Feed For Specific Animals (AREA)
Abstract
The present invention relates to field of feed additive technology, and in particular to a kind of to significantly improve Immune Function In Animals, digestion and absorption function and have effects that the functional feedstuff additive and preparation method thereof of fattening growth promoting.Pass through the screening of target plant：According to required active constituent, 4 kinds of Polygonum multiflower knotweed, fructus schisandrae, Radix Paeoniae Alba, yacon target plants are filtered out from the available 236 kinds of Chinese medicine of animal；Prepare the preparation of plant polyose and plant oligosaccharides and reduced sugar；According to the physilogical characteristics and demand of different animals, selects the plant extracts of different proportion to be mixed with appropriate carrier, product is prepared.
Description
Technical field
The present invention relates to field of feed additive technology, and in particular to one kind is for improving ruminant digestion absorption function
The feed addictive based on plant extracts preparation method.
Background technology
In recent years, it emerges one after another the problem of food security, food security increasingly causes society and the extensive concern of the common people.
Solving food-safety problem will pick up from source, and animal husbandry is directly related to the body of the mankind as one of source link
The significant problem of heart health, the stabilization and development of society, it is necessary to draw attention.And instantly, the inferior health of animal has become one kind
General state, though animal is without specific genius morbi, whole hypoimmunity, abilities of digestive and absorption is poor, and vigor reduces,
The animal of first-class cultivation index can not be fed using classic feed and method for breeding.
In order to solve the inferior health of animal, while ensureing the safety of animal foodstuff, best bet is exactly by improving poultry
The physiological function of fowl itself resists the influence of disease and various stress factors, while the digestion for improving feed nutrition substance is inhaled
It receives, so that nutriment is absorbed and utilized by animal body as far as possible.Using environmentally protective, nonreactive, the functionality of noresidue
Feed addictive is current commonplace mode, mainly passes through the growth of the digestive system, immune system that promote cultivated animals
Development, and then improve digestion and absorption function and the immune function of animal to reach diseases prevention growth promoting, improve the effect of meat.
Effect of the plant extracts as feed addictive has been obtained for widely approving at present, but existing extract
It obtains the big single mode for mostly using water extract-alcohol precipitation to carry out, it is low that there are recovery rates, and ingredient is single, and loss of effective components is big, uses
The shortcomings of high expensive, seriously affects promoting the use of for plant extracts.
Invention content
The goal of the invention of the present invention is：In view of the foregoing drawbacks, the present invention provides environmentally protective one kind, nonreactive, noresidue
Functional feedstuff additive, the present invention is in line with economic, efficient purpose, the characteristics of according to required active constituent, sieves meticulously
Target plant is selected to carry out compound collecting, operating process is concise, reasonable.Further according to the physilogical characteristics and demand of different ruminants,
Reasonable formula precisely adds, gives full play to product efficacy, solves that common Chinese additive additive amount is big, and bioavailability is low
The drawbacks of, ruminant digestion absorption function can be significantly improved.
The technical scheme is that choosing target plant Polygonum multiflower knotweed, Schisandra chinensis, Radix Paeoniae Alba, saussurea involucrata from 236 kinds of plants
Fruit carries out the orientation extraction of active ingredient further according to itself characteristic.Wherein, Polygonum multiflower knotweed and Schisandra chinensis compound collecting are lived
Property ingredient plant polyose, chlorogenic acid, plant flavone etc., Radix Paeoniae Alba, yacon compound collecting obtain the sugar such as plant fructooligosaccharide, reduced sugar
Substance.Further according to the physilogical characteristics and demand of different ruminants, the plant extracts of different proportion and appropriate carrier are selected
Mixing forms product.
Specifically, the present invention provides it is a kind of for improve ruminant digestion absorption function contain composite vegetables extractive
Feed addictive comprising 15-25 parts of plant polyoses, 0.5-10 parts of plant fructooligosaccharide, 0.5-3 parts of plant reductions sugar, carrier
62-90.5 parts.
Preferably, the present invention also provides a kind of feed addictives containing composite vegetables extractive comprising 10-15 parts
Plant polyose, 0.2-2 parts of plant fructooligosaccharide, 0.5-2 parts of plant reduction sugar, 81-89.3 parts of carrier.
Preferably, the used plant polyose of composite vegetables extractive feed addictive of the present invention be molecular weight in 3000-
5000 active plant polysaccharide；The ingredient of plant fructooligosaccharide is mainly fruit widow trisaccharide, fruit widow tetrose, fruit widow's pentasaccharides；Plant reduction
It is sugared then be the sugar containing reproducibility group in molecular structure, such as fructose, glucose.
Preferably, the carrier selected by the present invention is food yeast powder, cane molasses powder, maize cob meal, rice bran, swelling
The mixture of any one or a few in soil.
The present invention also provides a kind of feeds containing composite vegetables extractive for improving ruminant digestion absorption function
The preparation method of additive, includes the following steps：
(1) screening of target plant：According to required active constituent, filtered out from the available 236 kinds of Chinese medicine of animal
4 kinds of Polygonum multiflower knotweed, fructus schisandrae, Radix Paeoniae Alba, yacon target plants；
(2) preparation of plant polyose；After dry Polygonum multiflower knotweed, Schisandra chinensis are crushed with 0.6mm sieves, added in container
The water of 6-8 times of weight is uniformly mixed, and impregnates 1h, and reheating is boiled, and controls-90 DEG C of temperature 70 C, is kept 2-3h, is cooled to
40-50 DEG C, adjusting pH value is 5-7, and mixed enzyme is added, and digests 3-4h, and centrifugal filtration adds when filtrate is concentrated into the 1/4 of total amount
95% ethyl alcohol stands 1-2 hours, centrifugation, must precipitate simultaneously drying and crushing, obtain extract crude product；Extract crude product is distilled
Water is fully dissolved into the aqueous solution of 1~5wt%, first carries out ultrafiltration with the ultrafiltration membrane of molecular cut off Da 5000, molecule can be obtained
Trapped fluid of the amount more than 5000 and molecular weight are less than 5000 permeate, and then the polysaccharide solution by molecular weight less than 5000 passes through again
The ultrafiltration membrane treatment that molecular cut off is 3000 is crossed to get the polysaccharide solution for being 3000-5000 to molecular weight, solution is by spraying
Polyoses extract is obtained after drying.Gained plant polyose content is measured using phenol-sulfuric acid and colorimetric method, and operating procedure is shown in annex 1.
The mixed enzyme additive amount is the 0.1-2.0% of mixed material total weight.
The ingredient of the mixed enzyme includes：Cellulase, hemicellulase, pectase, amylase.The complex enzyme is by fibre
The plain enzyme of dimension, hemicellulase, pectase, amylase in mass ratio 3: 2: 2：2 compositions.
(3) preparation of plant oligosaccharides and reduced sugar：Fresh Radix Paeoniae Alba root, yacon are cleaned up, input rotating speed is
The hammer mill of 1200-1600r/min is broken into the particle of 1-3mm, is placed in fermentation tank, and 2 times of amounts are added by weight of material
Water, mixing, adjust pH5~6 be separately added into 5-8%'s into fermentation tank by percent by volume controlled at 20~40 DEG C
Black-koji mould, the S. cervisiae of 1-4%, 1-3% Bacillus acidi lactici microbial inoculum, and continuously stir 1-2h, stop stirring later, protect
Warm 15~25h of fermented and cultured sterilizes, and plant oligosaccharides and reduced sugar medicinal extract is made in concentration；Wherein oligosaccharides be mainly fruit widow trisaccharide,
Few five pools of fruit widow tetrose, fruit, plant reduction sugar is the sugar containing reproducibility group in molecular structure, predominantly fructose, glucose
Deng.Gained plant fructooligosaccharide content uses high effective liquid chromatography for measuring, content of reducing sugar to use DNS colorimetric method for determining；
(4) according to the physilogical characteristics and demand of different ruminants, the plant extracts of different proportion is selected to be carried with appropriate
Body mixes, and forms product.
The technique effect of the present invention：
The plant polyose that composite vegetables extractive feed addictive of the present invention includes is mainly molecular weight 3000-5000
Active polysaccharide, there is extremely strong immunocompetence, can be remarkably reinforced body fluid and cellular immune function, stimulating expression of macrophage swallows work(
Can, reinforce T, B cell proliferation, promote the growth and development of immune organ, enhance body's immunity；Plant fructooligosaccharide is mainly
Fruit widow trisaccharide, fruit widow tetrose, fruit widow's pentasaccharides itself can not be that animal body absorbs, but can be used as probiotic, by being prebiotic
Bacterium provides nutrition, promotes growth of probiotics, inhibits harmful bacteria growth, while can promote intestines peristalsis, remove enteron aisle rubbish, changes
Kind functions of intestines and stomach；Plant reduction sugar is the sugar containing reproducibility group in molecular structure, has extremely strong antioxidant activity, can
Free radical is removed, maintains red blood cell in animal body to be in reduction-state, oxidative damage, does not significantly increase resisting stress capability.
Description of the drawings
Fig. 1 is the preparation technology flow chart of the present invention.
Specific implementation mode
With reference to embodiment, the present invention is described in detail.
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that described herein, specific examples are only used to explain the present invention, is not used to limit
The fixed present invention.
Composite vegetables extractive feed addictive prepares embodiment
(1) screening of target plant：According to required active constituent, 4 are filtered out from the available 236 kinds of Chinese medicine of animal
Kind target plant Polygonum multiflower knotweed, Schisandra chinensis, Radix Paeoniae Alba, yacon.
(2) preparation of plant polyose：After dry Polygonum multiflower knotweed, Schisandra chinensis are crushed with 0.6mm sieves, added in container
The water of 6-8 times of weight is uniformly mixed, and impregnates 1h, and reheating is boiled, and controls -90 DEG C of temperature 70 C, is kept 2-3h, is cooled to
40-50 DEG C, adjusting pH value is 5-7, and mixed enzyme is added, and digests 3-4h, and centrifugal filtration adds when filtrate is concentrated into the 1/4 of total amount
95% ethyl alcohol stands 1-2 hours, centrifugation, must precipitate simultaneously drying and crushing, obtain extract crude product；Extract crude product is distilled
Water is fully dissolved into the aqueous solution of 1~5wt%, first carries out ultrafiltration with the ultrafiltration membrane of molecular cut off 5000Da, molecule can be obtained
Trapped fluid of the amount more than 5000 and molecular weight are less than 5000 permeate, and then the polysaccharide solution by molecular weight less than 5000 passes through again
The ultrafiltration membrane treatment that molecular cut off is 3000 is crossed to get the polysaccharide solution for being 3000-5000 to molecular weight, solution is by spraying
Polyoses extract is obtained after drying.The mixed enzyme additive amount is the 0.1-2.0% of mixed material total weight.
The ingredient of the mixed enzyme includes：Cellulase, hemicellulase, pectase, amylase.The complex enzyme is by fibre
The plain enzyme of dimension, hemicellulase, pectase, amylase in mass ratio 3：2：2：2 compositions.
(3) preparation of plant oligosaccharides and reduced sugar：Fresh Radix Paeoniae Alba root, yacon are cleaned up, input rotating speed is
The hammer mill of 1200-1600r/min is broken into the particle of 1-3mm, is placed in fermentation tank, and 2 times of amounts are added by weight of material
Water, mixing, adjust pH5~6 be separately added into 5-8%'s into fermentation tank by percent by volume controlled at 20~40 DEG C
Black-koji mould, the S. cervisiae of 1-4%, 1-3% Bacillus acidi lactici microbial inoculum, and continuously stir 1-2h, stop stirring later, protect
Warm 15~25h of fermented and cultured sterilizes, and plant oligosaccharides and reduced sugar medicinal extract is made in concentration；
(4) preparation of feed addictive：The plant extracts of different proportion is mixed with suitable carrier, forms product.
Plant extracts according to the form below requirement prepared by 1 step 3 of embodiment forms feed addictive with carrier combination：
| Plant polyose | Plant fructooligosaccharide | Plant reduction sugar | Carrier | |
| Ruminant | 10-15 parts | 0.2-2 parts | 0.5-2 parts | 81~89.3 parts |
Classification by product, it is as follows for the usage and dosage of different animals：
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.
Claims (7)
1. a kind of preparation method for improving the feed addictive containing composite vegetables extractive of ruminant digestion absorption function,
Include the following steps：
(1) screening of target plant：According to required active constituent, night friendship is filtered out from the available 236 kinds of Chinese medicine of animal
4 kinds of rattan, fructus schisandrae, Radix Paeoniae Alba, yacon target plants；
(2) preparation of plant polyose：Using Polygonum multiflower knotweed, Schisandra chinensis by crushing, impregnating, boiling is added to boil, enzymolysis, filtering, extraction
It obtains；
(3) preparation of plant oligosaccharides and reduced sugar：It is concentrated to give through broken, fermentation, sterilizing using Radix Paeoniae Alba root, yacon；
(4) according to the physilogical characteristics and demand of different ruminants, select the plant extracts of different proportion and appropriate carrier mixed
It closes, obtains feed addictive.
2. a kind of feed containing composite vegetables extractive for improving ruminant digestion absorption function as described in claim 1
The preparation method of additive, it is characterised in that step (2) is：After dry Polygonum multiflower knotweed, Schisandra chinensis are crushed with 0.6mm sieves,
The water that 6-8 times of weight is added in container is uniformly mixed, and impregnates 1h, and reheating is boiled, and controls -90 DEG C of temperature 70 C, is kept
2-3h are cooled to 40-50 DEG C, and adjusting pH value is 5-7, and mixed enzyme is added, and digests 3-4h, centrifugal filtration, and filtrate is concentrated into always
Amount 1/4 when, add 95% ethyl alcohol, stand 1-2 hour, centrifugation must precipitate simultaneously drying and crushing, obtain extract crude product；It will extraction
Object crude product is fully dissolved into the aqueous solution of 1~5wt% with distilled water, is first surpassed with the ultrafiltration membrane of molecular cut off Da 5000
Filter can be obtained the permeate of trapped fluid of the molecular weight more than 5000 and molecular weight less than 5000, then again be less than molecular weight
The ultrafiltration membrane treatment that 5000 polysaccharide solution is 3000 by molecular cut off is to get the polysaccharide for being 3000-5000 to molecular weight
Solution, solution obtain polyoses extract after spray drying.
A kind of containing composite vegetables extractive for improve ruminant digestion absorption function 3. as claimed in claim 1 or 2
The preparation method of feed addictive, it is characterised in that step (3) is：Fresh Radix Paeoniae Alba root, yacon are cleaned up, input turns
Speed is the hammer mill of 1200-1600r/min, is broken into the particle of 1-3mm, is placed in fermentation tank, and 2 are added by weight of material
The water of amount again, mixing adjust pH5~6 and are separately added into 5- into fermentation tank by percent by volume controlled at 20~40 DEG C
8% black-koji mould, the S. cervisiae of 1-4%, 1-3% Bacillus acidi lactici microbial inoculum, and continuously stir 1-2h, stop stirring later
It mixes, heat-preservation fermentation 15~25h of culture, sterilizes, plant oligosaccharides and reduced sugar medicinal extract is made in concentration.
4. as described in any one of claims 1-3 a kind of for improving being carried containing composite plant for ruminant digestion absorption function
Take the preparation method of object feed addictive, it is characterised in that mixed enzyme additive amount described in step (2) is mixed material total weight
0.1-2.0%.
5. according to any one of claims 1-4 a kind of for improving being carried containing composite plant for ruminant digestion absorption function
Take the preparation method of object feed addictive, it is characterised in that complex enzyme described in step (2) by cellulase, hemicellulase,
Pectase, amylase in mass ratio 3: 2: 2：2 compositions.
6. by a kind of for improving being raised containing composite vegetables extractive for ruminant digestion absorption function described in claim 1-5
Feed addictive prepared by the preparation method of feed additives, it is characterised in that：Include 10-15 parts of plant polyose, 0.2-2 parts
Plant fructooligosaccharide, 0.5-2 parts plant reduction sugar, 81-89.3 parts of carrier.
7. the composite vegetables extractive feed addictive described in claim 6 is in terms of improving ruminant digestion absorption function
Using.
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Citations (7)
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|---|---|---|---|---|
| CN104173436A (en) * | 2013-09-02 | 2014-12-03 | 郑州后羿制药有限公司 | Compound traditional Chinese medicinal composition for cows, and preparation method and application thereof |
| CN104381662A (en) * | 2014-11-17 | 2015-03-04 | 天长市彭氏羊业有限公司 | Special feed capable of promoting digestion for later-period fattening sheep |
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| CN104522349A (en) * | 2014-11-28 | 2015-04-22 | 王立才 | Fattening cattle feed |
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| CN106615756A (en) * | 2017-02-24 | 2017-05-10 | 安徽金牧饲料有限公司 | Sheep feed capable of promoting digestion |
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2018
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| CN104173436A (en) * | 2013-09-02 | 2014-12-03 | 郑州后羿制药有限公司 | Compound traditional Chinese medicinal composition for cows, and preparation method and application thereof |
| CN104381662A (en) * | 2014-11-17 | 2015-03-04 | 天长市彭氏羊业有限公司 | Special feed capable of promoting digestion for later-period fattening sheep |
| CN104431478A (en) * | 2014-11-26 | 2015-03-25 | 枞阳县兴龙综合养殖有限公司 | Beef cattle fattening feed with high energy utilization ratio and preparation method of beef cattle fattening feed |
| CN104522349A (en) * | 2014-11-28 | 2015-04-22 | 王立才 | Fattening cattle feed |
| CN104782930A (en) * | 2015-03-13 | 2015-07-22 | 安徽省固镇县争华羊业有限公司 | Hu sheep feed for digestion promotion and immunity enhancement, and preparation method thereof |
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