CN108372162A - A kind of separation gel blocks the processing method of medical laboratory's testing instruments Measurement channel - Google Patents
A kind of separation gel blocks the processing method of medical laboratory's testing instruments Measurement channel Download PDFInfo
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- CN108372162A CN108372162A CN201810163497.7A CN201810163497A CN108372162A CN 108372162 A CN108372162 A CN 108372162A CN 201810163497 A CN201810163497 A CN 201810163497A CN 108372162 A CN108372162 A CN 108372162A
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- separation gel
- measurement channel
- separation
- gel
- testing instruments
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/027—Cleaning the internal surfaces; Removal of blockages
Abstract
A kind of separation gel blocks the processing method of medical laboratory's testing instruments Measurement channel.Have the characteristics that thixotropy and separation gel viscosity-temperature curve first with separation gel, it promotes separation gel temperature and applies the principle that centrifugal force makes separation gel by gel state for low viscosity fluid collosol state, with physics method, centrifugation throws away the separation gel that the overwhelming majority is blocked in testing instruments Measurement channel.Second step uses chemical method, and the separation gel thin layer remained on testing instruments Measurement channel inner wall is rinsed out with nonpolar solvent dissolving in draught cupboard.
Description
Technical field
The present invention relates to field of medical examination, specifically a kind of separation gel, which blocks medical laboratory's testing instruments and measures, to be led to
The processing method in road.
Background technology
Serum separation gel has thixotropic mucus colloid, structure by what hydrophobic organic compound and Cab-O-sil formed
In contain a large amount of hydrogen bonds, due to hydrogen bond association formed reticular structure, under the influence of centrifugal force reticular structure be destroyed
Become the low fluid of viscosity, form reticular structure again after centrifugal force disappears, this property is referred to as thixotropy
（thixotropy）.I.e. in the case of temperature-resistant, certain mechanical force is applied to this mucus colloid, it can be from highly viscous
Gel state becomes the collosol state of low viscosity, if mechanical force, which disappears, restores original highly viscous gel state again.Specifically
It says：Hydrogen bond not only forms single Gong Jia Key, under certain condition it also with one weak hydrogen of the intermolecular formation of other negative electrical charges
Key, at normal temperatures hydrogen bond be easier be cut off cause in conjunction with.Silica surface has silicone hydroxyl（SiOH）, form SiO points
Sub- condensate（Primary particle）, chain combination particle is connected to hydrogen bond between this primary particle.This chain silica particle
Further hydrogen bond is formed between the particle for the hydrophobic organic compound for constituting separation gel and generates reticular structure, is constituted to have and be touched
The gel molecular of change.By the thixotropy feature of separation gel, separation gel test tube is now widely used in clinical medicine, for facing
Bed examines the separation and Extraction of serum specimen in work.
Separation gel proportion maintains 1.05, and serum ratio weighs about 1.02, and clot ratio weighs about 1.08, when separation gel and coagulates
When Gu blood after centrifuges in same test tube, and cause the hydrogen link network in silica condensate due to applying centrifugal force to separation gel
Shape is destructurized to become chain structure, and separation gel just becomes the low substance of viscosity, and the clot than separation gel weight is moved to pipe
Flop phenomenon occurs for bottom, separation gel, and bottom of the tube forms three layers of clot, separation gel and serum.It is lost when centrifuge stops operating
After centrifugal force, reticular structure is constituted by hydrogen bond again between the chain particle of silica condensate in separation gel, restores initial high viscosity
Gel state forms separation layer between serum and clot.
When making separation gel, thixotropic property is a most important index for each manufacturer, when separation gel touches
When becoming poor performance, under the action of the centrifugal force, it is molten that separation gel can not flexibly become suitable low viscosity from highly viscous gel state
Gluey state, liquefaction effect is poor, deformation energy force difference, and the effect that serum and clot is isolated is also poor, can not ensure the normal of serum specimen
Separation and Extraction influences the accuracy of clinical test results.When the thixotropic property of separation gel is strong, under the action of the centrifugal force, separation
Dispergation effect is good, and deformability is strong, and the separation layer boundary that separation gel is formed between serum and clot is clear, can be very good
Separation and Extraction serum specimen.But the separation gel thixotropy the strong also more to be easy to happen floating oil phenomenon, i.e., it is difficult that naked eyes are formed in serum
With the fine oil droplets particle seen, when testing instruments continue working, these fine oil droplets particles can gradually adhere to accumulation and block
Measurement channel.
Temperature has a significant impact to the viscosity of separation gel, and separation adhesiveness and temperature curve announcement temperature are higher, point
It is lower from adhesiveness.Separation gel in process of clinical application, room temperature is higher or centrifuge temperature it is higher in the case of, separation gel meeting
Floating oil phenomenon more easily occurs, it is easier to the problem of separation gel adherency accumulation blocks testing instruments Measurement channel occur.
The processing method of testing instruments Measurement channel problem is blocked for separation gel, some laboratories utilize separation adhesiveness
With temperature curve feature, with boiling water or with hot water strong flushing Measurement channel repeatedly, although at that time apparently can be basic
It solves the problems, such as, is continuing with, but since separation adhesivity is extremely strong, and depending merely on promotion temperature can not make separation gel liquefy completely,
It is always remained on the inner wall of Measurement channel, having more than is needed, how long separation gel will adhere to accumulation and block again；Also
Some laboratory sides are dredged with finer wire needle, while being rushed with hot water, can cause Measurement channel inner wall mechanical damage in this way；Reality also
It tests room and directly dissolves flushing blocking Measurement channel with nonpolar solvent, but nonpolar solvent toxicity is larger, is not suitable for for a long time
Use, and usually block Measurement channel separation gel area and the scale of construction it is larger, be applied alone nonpolar solvent to dissolve flushing repeatedly, no
It is that can handle well the short time, increases unexpected injury risk.We have invented a kind of separation gels to block medicine reality thus
Test the processing method of room testing instruments Measurement channel.
Invention content
The present invention provides to solve the drawbacks of medical laboratory instrument Measurement channel is not easy thorough cleaning by separation gel blocking
A method of simply, thoroughly removing blocking measurement pipeline separation gel.Its technical characteristic is to have thixotroping first with separation gel
Property and the characteristics of separation adhesiveness and temperature curve, that is, promote separation gel temperature and application centrifugal force can make separation gel by
Gel state is the principle of low viscosity fluid collosol state, and with physics method, centrifugation throws away the overwhelming majority and is blocked in inspection
Test the separation gel in apparatus measures channel.Specific is exactly to be detached described the hot water of addition certain temperature in the test tube
The testing instruments Measurement channel measurement module that glue blocks disassembles, and the general L-shaped or staight needle type of the Measurement channel is
Stainless steel, the Measurement channel internal diameter is thinner, when separation gel gradually adheres to the import one that accumulation blocks " L " type Measurement channel
It can alarm when one section of the needle point of section or staight needle type Measurement channel.The separation gel of the testing instruments Measurement channel is blocked into Duan Quan
Portion is immersed in the hot water in test tube, and the input end of Measurement channel should be inserted into close to the examination by " L " the type Measurement channel
The bottom of pipe cannot bottom out, the outlet end of " L " the type Measurement channel, i.e. another " right-angle side " of " L " type, to be fixed on from
On heart cup, " L " type Measurement channel is made to be remained stationary in high speed centrifugation；The staight needle type Measurement channel should select band rubber
The needle tip of staight needle type Measurement channel is passed through rubber stopper center, is inserted into close to the bottom of the test tube by the test tube of plug, cannot
It bottoms out, the staight needle type Measurement channel keeps it fixed in high speed centrifugation by rubber stopper fixed position.Centrifuge is set
It sets certain rotating speed and the time starts to work, wait for that centrifugation finishes, be blocked in the separation gel overwhelming majority meeting of Measurement channel originally
It is deposited on the test tube bottom with gel state.The second step uses chemical method：It is dissolved and is rushed with nonpolar solvent in draught cupboard
Wash off the separation gel thin layer remained on testing instruments Measurement channel inner wall.
To verify separation gel elimination effect, we design following contrast experiment：Access root and testing instruments Measurement channel mouth
Glass straight tube similar in diameter, often pipe be manually added in the separation gel to glass tube of equivalent from one end, simulation separation gel block examine
Apparatus measures channel status, according to method described above step remove separation gel and do it is various under the conditions of separation gel elimination effect
Comparison.The separation gel that we use in experimentation is U.S. company BD product.
By series of experiments, confirm：
（a）Influence of the temperature to separation gel elimination effect；
（b）The influence of centrifuge speed and time to separation gel elimination effect；
（c）After selecting suitable centrifuge speed, time and hot water temperature, simple physical method is removed to blocking pipeline separation gel
The evaluation of effect；
（d）After physics method removing is blocked in the separation gel of glass tube, glass inside pipe wall is remained in nonpolar solvent removing
On separation gel thin layer effect assessment.
Separation gel elimination effect judgment method and standard：
（Ⅰ）Physics method, which is removed, blocks the judgement of glass tube separation gel effect：Simple physical method is disposed, because of separation gel
There is oiliness, can be reflective, naked eyes can be seen whether separation gel on glass tube inner wall has residual, is sticked to after being centrifuged by precise
The gel state separation gel of test tube bottom can be evaluated simple physical method with accurate quantitative analysis and remove the removing for blocking pipeline separation gel
Effect；
（Ⅱ）Chemical method, which is removed, blocks the judgement of glass tube separation gel effect：It is in that the blue sensitivity reacted is very high that starch, which meets iodine, can
For use as the qualitative method of discriminating starch.We judge that chemical method removes the effect of separation gel, tool indirectly using this principle
Body is exactly the glass tube removed simple physical method after separation gel, after drying, is rinsed out and is remained in nonpolar solvent
Separation gel thin layer on glass inside pipe wall.Glass inside pipe wall dries after being cleaned again with water, end is blocked from separation gel, by glass tube
Interior that a certain amount of starch dried noodle is added, the separation gel for blocking glass tube blocks end, and by this end under, the other end is upright certain upper
Then time is horizontally arranged glass tube on table top, starch dried noodle is laid in separation gel and blocks on one section of glass inside pipe wall, continuously
Certain time is rolled, until beginning to guarantee starch dried noodle eventually and separation gel one section of glass inside pipe wall of blocking to come into full contact with, then again
Glass tube is rinsed with water, washes out after not being adhered to the starch of glass inside pipe wall by separation gel, dries, one is added in glass tube
Quantitative 0.01%~0.05% dilute iodine solution makes 0.01%~0.05% dilute iodine solution and is adhered to the shallow lake of glass inside pipe wall by separation gel
Powder comes into full contact with reaction, on observation adherency one section of glass inside pipe wall of separation gel whether there is or not the blue or bluer depth, is sentenced with this
How much separation gel has noresidue and residual quantity in disconnected glass tube, removes assessing chemical method and blocks the effect of pipeline separation gel；
（Ⅲ）Chemical method removes the criterion for blocking glass tube separation gel effect：
（i）It is extremely strong to detach adhesivity, after the cleaning process, glass inside pipe wall remains the shallow lake that separation gel is more, is adhered to by separation gel
Powder is more, after iodine solution is added, develops the color on adherency one section of glass inside pipe wall of separation gel deeper, represents the removing of glass inside pipe wall separation gel
Effect is poor；
（ii）After the cleaning process, glass inside pipe wall residual separation gel is fewer, and the starch adhered to by separation gel is fewer, and iodine solution is added
Afterwards, it develops the color on adherency one section of glass inside pipe wall of separation gel more shallow, it is good to represent glass inside pipe wall separation gel elimination effect；
（iii）After the cleaning process, it is thoroughly removed without separation glue residua on glass inside pipe wall, starch is also remained without adherency, is added
After entering iodine solution, develops the color without change on glass inside pipe wall, it is best to represent elimination effect.
Experimental result：60~90 DEG C of hot water temperature, centrifuge 1170~2500g of power are centrifuged 5~30 minutes, you can
To dispose 80%~90% blocking Measurement channel separation gel, glass tube only remains a thin layer separation gel and is adhered on inside pipe wall,
Rinsing 2~6 times with nonpolar solvent simply dissolving can fully erased remaining separation gel.
Nonpolar solvent dichloromethane, chloroform, acetone, petroleum ether, also phenol+1,1,2,2- tetrachloroethanes, quality 1:
1 mixing, these modes can separated and dissolved glue.But these above-mentioned modes are all that toxicity is bigger, and usage time is had to
It is short, it to be operated in draught cupboard, we use acetone.
We handle most separation gels and block apparatus measures channel in real work, are Roche Holding Ag of U.S. COBAS
The sipper needles of 8000 measuring ion modules, so we see attached drawing as example.
Description of the drawings
Fig. 1 is that the separation gel of the present invention blocks the front schematic view of sipper needles.
In Fig. 1：1 sipper needles fixed platform, 2 sipper needles, 3 test tubes, 4 hot water, 5 separation gels, 6 sipper needles
Import, the outlet of 7 sipper needles.
Fig. 2 is that the sipper needle Physicals of the present invention remove the design sketch after blocking separation gel.
In fig. 2：1 sipper needles fixed platform, 2 sipper needles, 3 test tubes, 4 hot water, 5 separation gel thin layers, 6
The import of sipper needles, 7 separation gels, the outlet of 8 sipper needles.
Fig. 3 is separation adhesiveness and temperature curve figure.
Fig. 4 is that staight needle type Measurement channel separation gel blocks front schematic view.
In Fig. 4：1 staight needle type Measurement channel, 2 rubber stoppers, 3 test tubes, 4 hot water, 5 separation gels, 6 staight needle type Measurement channel needles
Point.
Specific implementation mode
With reference to the accompanying drawings and examples, with " L " type Measurement channel and staight needle type Measurement channel respectively to the present invention do into
One step explanation.
As depicted in figs. 1 and 2, when medical laboratory's testing instruments work, floating oil, serum occur for separation gel serum specimen
The fine oil droplets particle that middle formation naked eyes are difficult to see that, gradually adherency accumulation block sipper needles import 1, cause instrument can not be normal
Work.Self-checking apparatus measures module unloads sipper needles 2, and 60~90 DEG C of enough hot water are added in clean tube, will
Sipper needles import 6 is inserted into test tube 3, close to 3 bottom of test tube, cannot be bottomed out, and ensures that 6 separation gel of sipper needles import blocks
One section is totally immersed into hot water, is put into centrifuge, and sipper needles, which are exported 7 one sections, to be fixed on adhesive tape on Centrifuge Cup, setting
Centrifugal force is 1170~2500 g, is centrifuged 5~10 minutes, and sipper needles 2 are taken out, and dries remaining hot water, after to be dried, is divulging information
In cupboard, rinsed repeatedly with acetone 2~6 times can the fully erased remaining separation gel film of 2 inner wall of sipper needles, then use distilled water
It rinses well, dries repeatedly, the sipper needles 2 cleaned up are reinstalled back to original position of testing instruments, again initially
Change instrument, starts to work.
As shown in figure 4, when medical laboratory's testing instruments work, floating oil occurs for separation gel serum specimen, shape in serum
At the fine oil droplets particle being visually difficult to see that, gradually adherency accumulation blocks 6 one sections of staight needle type Measurement channel needle point, cause instrument without
Method works normally.Self-checking apparatus measures module unloads staight needle type Measurement channel 1, is added in band rubber stopper clean tube enough
60~90 DEG C of hot water, cover tightly rubber stopper, by staight needle type Measurement channel needle point 6 pass through test tube rubber stopper center, be inserted into test tube 3
In, it close to 3 bottom of test tube, cannot bottom out, and ensure that 6 separation gel of staight needle type Measurement channel needle point blocks one section and is totally immersed into hot water
In, it is put into centrifuge, sets centrifugal force as 1170~2500 g, centrifuge 5~10 minutes, take out staight needle type Measurement channel 1, get rid of
Dry remaining hot water, after to be dried, in draught cupboard, rinsing 2~6 times repeatedly with acetone can fully erased staight needle type Measurement channel
The remaining separation gel film of 1 inner wall, then rinsed well repeatedly with distilled water, it dries, the staight needle type Measurement channel 1 that will be cleaned up
The original position for reinstalling back testing instruments, reinitializes instrument, starts to work.
Claims (2)
1. a kind of separation gel blocks the processing method of medical laboratory's testing instruments Measurement channel, it is characterised in that：First with
Physics method promotes separation gel temperature to 60~90 DEG C, applies 1170~2500g of centrifugal force, centrifuge 5~10 minutes, make
Separation gel is low viscosity fluid collosol state by gel state, and centrifugation throws away the overwhelming majority about 80%~90% and is blocked in inspection
The separation gel in apparatus measures channel.
2. it is characterized in that：It is rinsed out and is remained on testing instruments Measurement channel inner wall with the nonpolar solvent acetone solution
Separation gel thin layer.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810163497.7A CN108372162A (en) | 2018-02-27 | 2018-02-27 | A kind of separation gel blocks the processing method of medical laboratory's testing instruments Measurement channel |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810163497.7A CN108372162A (en) | 2018-02-27 | 2018-02-27 | A kind of separation gel blocks the processing method of medical laboratory's testing instruments Measurement channel |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1539161A (en) * | 2001-08-06 | 2004-10-20 | ���Ͽع�����˾ | Process and apparatus for treating workpiece such as semionductor wafer |
| CN101217102A (en) * | 2007-01-04 | 2008-07-09 | 北京北方微电子基地设备工艺研究中心有限责任公司 | A method to remove surface contaminations on surfaces of semiconductor accessories |
| CN101944476A (en) * | 2009-07-09 | 2011-01-12 | 中芯国际集成电路制造(上海)有限公司 | Wafer washing method |
| CN102039281A (en) * | 2009-10-21 | 2011-05-04 | 中芯国际集成电路制造(上海)有限公司 | Method for cleaning wafer bonding pad surface |
| CN204148188U (en) * | 2014-05-30 | 2015-02-11 | 昆山中士设备工业有限公司 | Screw rod eccentric cleaning device |
-
2018
- 2018-02-27 CN CN201810163497.7A patent/CN108372162A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1539161A (en) * | 2001-08-06 | 2004-10-20 | ���Ͽع�����˾ | Process and apparatus for treating workpiece such as semionductor wafer |
| CN101217102A (en) * | 2007-01-04 | 2008-07-09 | 北京北方微电子基地设备工艺研究中心有限责任公司 | A method to remove surface contaminations on surfaces of semiconductor accessories |
| CN101944476A (en) * | 2009-07-09 | 2011-01-12 | 中芯国际集成电路制造(上海)有限公司 | Wafer washing method |
| CN102039281A (en) * | 2009-10-21 | 2011-05-04 | 中芯国际集成电路制造(上海)有限公司 | Method for cleaning wafer bonding pad surface |
| CN204148188U (en) * | 2014-05-30 | 2015-02-11 | 昆山中士设备工业有限公司 | Screw rod eccentric cleaning device |
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Application publication date: 20180807 |