CN108195744A - A kind of detection method of particulate matter - Google Patents
A kind of detection method of particulate matter Download PDFInfo
- Publication number
- CN108195744A CN108195744A CN201810258544.6A CN201810258544A CN108195744A CN 108195744 A CN108195744 A CN 108195744A CN 201810258544 A CN201810258544 A CN 201810258544A CN 108195744 A CN108195744 A CN 108195744A
- Authority
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- China
- Prior art keywords
- filter membrane
- particulate matter
- water
- detection method
- rinsed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000013618 particulate matter Substances 0.000 title claims abstract description 40
- 238000001514 detection method Methods 0.000 title claims abstract description 25
- 239000012528 membrane Substances 0.000 claims abstract description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- 238000007689 inspection Methods 0.000 claims abstract description 15
- 229920002678 cellulose Polymers 0.000 claims abstract description 10
- 239000001913 cellulose Substances 0.000 claims abstract description 10
- 238000011010 flushing procedure Methods 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 10
- 239000008213 purified water Substances 0.000 claims abstract description 10
- 238000005374 membrane filtration Methods 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims description 8
- 239000011859 microparticle Substances 0.000 claims description 6
- 230000003321 amplification Effects 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 229940079593 drug Drugs 0.000 claims description 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 6
- 208000003464 asthenopia Diseases 0.000 abstract description 2
- 239000008187 granular material Substances 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 208000007536 Thrombosis Diseases 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention proposes a kind of detection method of particulate matter, including step：(1) the desired amount of purified water is taken, through membrane filtration for several times, filtered purified water is collected, as particulate matter inspection water；(2) black filter membrane is rinsed with the water that step (1) obtains, made on filter membrane without apparent more than 5um particulate matters；(3) sample is rinsed, and collect flushing liquor with the water obtained with step (1)；The black filter membrane rinsed of the flushing liquor Jing Guo step (2) is filtered；(4) the black filter membrane after step (3) is positioned under big field microscope and counted.The detection method of the particulate matter of the present invention makes particulate matter be more easy to judge, and reduce reflective reduction eye fatigue using black background filter membrane.The filter membrane of cellulose mixture fat material is used in the method for the present invention, after insoluble granule is stayed on film, trace can be formed, improve detection accuracy.
Description
Technical field
The present invention relates to field of medical technology, and in particular to a kind of detection method of particulate matter.
Background technology
Since medical drug, medical equipment or medical apparatus cannot be guaranteed clean room in process of production, when note
Liquid, medical instrument are penetrated when entering blood, micronic dust, particle etc. may enter blood circulation system.And particulate matter enters
There is the potential danger for causing blood coagulation and thrombus after the circulatory system of human body.Therefore, into the circulatory system and the product of contacting blood
Need to detect the content of its particulate matter.The microscope count method of traditional detection particulate matter uses ordinary white to filter
Film is difficult to judge, and white reflection can increase eye fatigue when transparent or white particulate matter is observed, reduces effect
Rate.Particle is easily lost, moves after filtering, causes counting error.In order to solve the above technical problems, the present invention comes therefrom.
Invention content
Present invention seek to address that existing for prior art detection medical drug, medical equipment or medical apparatus particulate matter
Deficiency provides a kind of convenient for counting, the technical method of raising precision.
The purpose of the present invention is what is be achieved through the following technical solutions：A kind of detection method of particulate matter, including
Following steps：
(1) prepare particulate matter inspection water：The desired amount of purified water is taken, through membrane filtration for several times, until being used for
The quantity of particulate matter is less than acceptable level on the filter membrane of filter, filtered purified water is collected, as particulate matter
Inspection water；
(2) filter membrane is washed：Black filter membrane is rinsed, and observe under the microscope with the water that step (1) obtains, make nothing on filter membrane
Apparent more than 5um particulate matters, influence of the removal filter membrane residual particulates to detection；
(3) the sample preparation stage：Sample is rinsed, and collect flushing liquor with the water obtained with step (1)；By flushing liquor by walking
Suddenly the black filter membrane rinsed well of (2) is filtered, and filter is rinsed with water with appropriate particulate matter inspection again after filtering
The wall of device；
(4) the black filter membrane after step (3) is positioned under big field microscope and counted.
In currently preferred technical solution, in step (1), the membrane filtration through 0.4um is for several times.
In currently preferred technical solution, the black filter membrane is the filter membrane of black cellulose mixture fat material.
In currently preferred technical solution, in step (4), amplification factor is modulated into 100 times of progress counting micro particles.
In currently preferred technical solution, the sample includes medical drug, medical equipment or medical apparatus.
In currently preferred technical solution, a kind of detection method of particulate matter includes the following steps：
(1) prepare particulate matter inspection water：Take the desired amount of purified water, the membrane filtration through 0.4um for several times, until
The quantity of particulate matter is less than acceptable level on the filter membrane of filtering, filtered purified water is collected, as insoluble
Property microparticles with water be used for particulate determination；
(2) filter membrane is washed：The filter membrane of black cellulose mixture fat material is rinsed with water with particulate matter inspection, and aobvious
Micro- Microscopic observation makes on filter membrane without apparent more than 5um particulate matters, influence of the removal filter membrane residual particulates to detection；
(3) the sample preparation stage：Sample is rinsed with water, and collect flushing liquor with particulate matter inspection；Flushing liquor is passed through
The cellulose mixture fat filter membrane rinsed well is filtered, and filter is rinsed with water with appropriate particulate matter inspection again after filtering
The wall of device；
(4) the black cellulose mixture fat filter membrane is positioned under big field microscope and counted, amplification factor is modulated 100
Counting micro particles are carried out again.
The detection method of the particulate matter of the present invention is white or the particulate matter of light colour in detection primary color
When, usually white filter membrane is abandoned, using black background filter membrane, particulate matter is made to be more easy to judge, and reduce reflective reduction eyes
Fatigue.The filter membrane of cellulose mixture fat material is used in the method for the present invention, after insoluble granule is stayed on film, print can be formed
Mark, convenient for identification, and particulate matter is not easily shifted and loses, and improves detection accuracy.The present invention uses NIST
Traceable ParticleSize Standard particulate matters standard sample carries out method validation, verifies detection method
Accuracy.
Description of the drawings
Fig. 1 is the flow chart of one embodiment of detection method of the particulate matter of the present invention.
Specific embodiment
To further understand the present invention, preferred embodiment of the present invention is described with reference to specific embodiment, but should
Work as understanding, these descriptions are only the feature and advantage rather than the limit to the claims in the present invention further illustrated the present invention
System.
Said program is described further below in conjunction with specific embodiment.The implementation condition used in embodiment can root
Further adjustment is done according to the condition of specific producer, the implementation condition being not specified is usually the condition in routine experiment.
Embodiment 1
A kind of detection method of particulate matter, includes the following steps：
(1) prepare particulate matter inspection water：Take the desired amount of purified water, the membrane filtration through 0.4um for several times, until
The quantity of particulate matter is less than acceptable level on the filter membrane of filtering, filtered purified water is collected, as insoluble
Property microparticles with water be used for particulate determination；
(2) filter membrane is washed：The filter membrane of black cellulose mixture fat material is rinsed with water with particulate matter inspection, and aobvious
Micro- Microscopic observation makes on filter membrane without apparent more than 5um particulate matters, influence of the removal filter membrane residual particulates to detection；
(3) the sample preparation stage：Sample is rinsed with water, and collect flushing liquor with particulate matter inspection；Flushing liquor is passed through
The cellulose mixture fat filter membrane rinsed well is filtered, and filter is rinsed with water with appropriate particulate matter inspection again after filtering
The wall of device；
(4) the black cellulose mixture fat filter membrane is positioned under big field microscope and counted, amplification factor is modulated 100
Counting micro particles are carried out again；
(5) using NIST Traceable Particle Size Standard particulate matter standard samples progress side
Method is verified.
The technology contents and technical characteristic of the present invention have revealed that as above, however those skilled in the art still may base
Make various replacements and modification without departing substantially from spirit of the present invention, therefore, the scope of the present invention in teachings of the present invention and announcement
The revealed content of embodiment should be not limited to, and various replacements and modification without departing substantially from the present invention should be included, and is this patent Shen
Please claim covered.
Claims (5)
1. a kind of detection method of particulate matter, includes the following steps：
(1) prepare particulate matter inspection water：The desired amount of purified water is taken, through membrane filtration for several times, until for filtering
The quantity of particulate matter is less than acceptable level on filter membrane, filtered purified water is collected, as particulate matter inspection
Use water；
(2) filter membrane is washed：Black filter membrane is rinsed, and observe under the microscope with the water that step (1) obtains, made on filter membrane without apparent
More than 5um particulate matters, influence of the removal filter membrane residual particulates to detection；
(3) the sample preparation stage：Sample is rinsed, and collect flushing liquor with the water obtained with step (1)；Flushing liquor is passed through into step
(2) the black filter membrane rinsed well is filtered, and filter is rinsed with water with appropriate particulate matter inspection again after filtering
Wall；
(4) the black filter membrane after step (3) is positioned under big field microscope and counted.
2. detection method according to claim 1, which is characterized in that in step (1), the membrane filtration through 0.4um is for several times.
3. detection method according to claim 1, which is characterized in that the black filter membrane is black cellulose mixture fat material
The filter membrane of matter.
4. detection method according to claim 1, which is characterized in that in step (4), by amplification factor modulate 100 times into
Row counting micro particles.
5. detection method according to claim 1, which is characterized in that the sample include medical drug, medical equipment or
Medical apparatus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810258544.6A CN108195744A (en) | 2018-03-27 | 2018-03-27 | A kind of detection method of particulate matter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810258544.6A CN108195744A (en) | 2018-03-27 | 2018-03-27 | A kind of detection method of particulate matter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108195744A true CN108195744A (en) | 2018-06-22 |
Family
ID=62596195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810258544.6A Pending CN108195744A (en) | 2018-03-27 | 2018-03-27 | A kind of detection method of particulate matter |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108195744A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114324154A (en) * | 2020-09-30 | 2022-04-12 | 阅美测量系统（上海）有限公司 | Method for analyzing particles deposited on filter disc and sample preparation and analysis equipment thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005241325A (en) * | 2004-02-25 | 2005-09-08 | Wako Pure Chem Ind Ltd | Quantitative method of lipocalin-type prostaglandin d synthetic enzyme and reagent for quantification |
| CN203563564U (en) * | 2013-11-25 | 2014-04-30 | 中国农业科学院植物保护研究所 | Single fruit fly insect feeding device |
| CN103898005A (en) * | 2014-01-09 | 2014-07-02 | 浙江大学 | Electrochemically-active bacterium and screening method thereof |
| CN103940707A (en) * | 2014-05-06 | 2014-07-23 | 江苏苏净集团有限公司 | Method and device for counting insoluble particles |
| CN104345018A (en) * | 2014-06-04 | 2015-02-11 | 秦少平 | Detector-array-based fluid particle measuring instrument |
| CN104677785A (en) * | 2013-12-02 | 2015-06-03 | 上海微创医疗器械（集团）有限公司 | Insoluble particle testing system |
-
2018
- 2018-03-27 CN CN201810258544.6A patent/CN108195744A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005241325A (en) * | 2004-02-25 | 2005-09-08 | Wako Pure Chem Ind Ltd | Quantitative method of lipocalin-type prostaglandin d synthetic enzyme and reagent for quantification |
| CN203563564U (en) * | 2013-11-25 | 2014-04-30 | 中国农业科学院植物保护研究所 | Single fruit fly insect feeding device |
| CN104677785A (en) * | 2013-12-02 | 2015-06-03 | 上海微创医疗器械（集团）有限公司 | Insoluble particle testing system |
| CN103898005A (en) * | 2014-01-09 | 2014-07-02 | 浙江大学 | Electrochemically-active bacterium and screening method thereof |
| CN103940707A (en) * | 2014-05-06 | 2014-07-23 | 江苏苏净集团有限公司 | Method and device for counting insoluble particles |
| CN104345018A (en) * | 2014-06-04 | 2015-02-11 | 秦少平 | Detector-array-based fluid particle measuring instrument |
Non-Patent Citations (1)
| Title |
|---|
| 付艳: ""注射液中不溶性微粒检查方法"", 《中国粉体技术》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114324154A (en) * | 2020-09-30 | 2022-04-12 | 阅美测量系统（上海）有限公司 | Method for analyzing particles deposited on filter disc and sample preparation and analysis equipment thereof |
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| PB01 | Publication | ||
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180622 |
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| RJ01 | Rejection of invention patent application after publication |