CN108370825A - A kind of artificial culture method of Lasiosphaera fenzlii strain - Google Patents
A kind of artificial culture method of Lasiosphaera fenzlii strain Download PDFInfo
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- CN108370825A CN108370825A CN201611067554.9A CN201611067554A CN108370825A CN 108370825 A CN108370825 A CN 108370825A CN 201611067554 A CN201611067554 A CN 201611067554A CN 108370825 A CN108370825 A CN 108370825A
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Abstract
The invention discloses a kind of artificial culture method of Lasiosphaera fenzlii strain, agricultural technology fields, it is characterized in that being carried out by following step：One, strain prepares：Extract the aleurioconidium body in thalline entity；Two, culture medium raw material prepares：40~50 parts of drying bagasse, 20~30 parts of dry clay, 15~20 parts of fine sand；Three, culture medium is modulated：Bagasse is crushed, clay, fine sand are mixed into, being uniformly mixed becomes mixed culture material；Four, culture medium goes out miscellaneous bacteria；Five, culture medium forming is planted with strain：Mixed culture material after high-temperature sterilization is packed into plastic tub, surface sprinkles Lasiosphaera fenzlii strain conidia powder, spreading density：1g~1.5g/m2, then spread a thin layer mixed culture material；Six, overlay film moisture-heat preservation culture；Seven, keep 25 DEG C~30 DEG C of canopy temperature as the temperature for urging the mushroom phase during growth；Eight, the management during fruiting.
Description
Technical field
The present invention relates to a kind of artificial culture methods of Lasiosphaera fenzlii strain, belong to agricultural technology field.
Background technology
Lasiosphaera fenzlii Zoopagales (scientific name：Lycoperdales), a mesh of abdomen heterobasidium Gammaproteobacteria is fungal organisms, and scientific name is horse
Suddenly, ripe Lasiosphaera fenzlii is generally more smaller than the fist of adult.It is commonly called as " horsehit packet " due to shape, color exactly like horsehit.
Lasiosphaera fenzlii is generally grown in the litter of underground, pyrophilous high humidity environment, therefore generally has more present rainwater
Plentiful 7, August part is only suitable for existence in the preferable mountain area of ecological environment or upper level.Lasiosphaera fenzlii originate in China Inner Mongol, Henan,
The ground such as Hebei, Shanxi, Shaanxi, Gansu, Anhui, Jiangsu, Hubei.Chemical analysis：Phosphoric acid sodium, gemmatein (gemmatein,
C17H12O7), the amino acid and urea, lipoids etc. such as ergosterol, leucine (leucine), tyrosine (tyrosine).
Pharmacological action：There is mechanicalness anastalsis, has apparent anastalsis to oral hemorrhage, curative effect is no less than starch sea
Continuous or gelfoam the disadvantage is that not being absorbed by tissue, therefore should not be used as to retain hemostasis or dead space filling in tissue.The water of Lasiosphaera fenzlii
Preserved material has the dermatophytes such as Microsporon audouini, microsporum ferrugineum different degrees of inhibiting effect.
Lasiosphaera nipponica(Kawam.)Y.Kobayasi belongs to a mesh of abdomen heterobasidium Gammaproteobacteria, is fungal organisms, scientific name is Lasiosphaera fenzlii.Ripe Lasiosphaera fenzlii generally compares
The fist of adult is smaller.It is another to there is a kind of huge " mushroom " shape to belong to grey spore, Chinese medicine is done, for treating the diseases such as pharyngalgia, aphonia, external application can
Hemostasis, is a kind of natural resources of preciousness, has high medical value.
Lasiosphaera nipponica(Kawam.)Y.Kobayasi is the highest a kind of wild mushroom of plating efficiency on mountain, annual seven, in the August woods, from the grass, it is waste
Its figure can be found in ground.Tender Lasiosphaera nipponica(Kawam.)Y.Kobayasi inside and outside is white, delicious edible, can cook and eat, tender such as bean curd.But
More than the mushroom of Lasiosphaera nipponica(Kawam.)Y.Kobayasi deliciousness in Yunnan, so price is very cheap on the market for Lasiosphaera nipponica(Kawam.)Y.Kobayasi, usually buying can fry once, two-spot
A upper disk.Radish, Chinese cabbage are had his likes and dislikes, I is exactly one of bean vermicelli of Lasiosphaera nipponica(Kawam.)Y.Kobayasi, as long as Lasiosphaera nipponica(Kawam.)Y.Kobayasi cleaning is fairly simple to prune outside
Mycoderma can obtain the thalline of quail egg size or egg size, if the Lasiosphaera nipponica(Kawam.)Y.Kobayasi that peasant has cut can also be bought by not wanting to take the trouble,
It is stir-fried and eaten as long as simply cleaning to be sliced.The Lasiosphaera nipponica(Kawam.)Y.Kobayasi not only fresh and tender but also also certain medical value of taste.Lasiosphaera fenzlii
It is neutral in nature and sweet in taste, it is nontoxic.Containing Argine Monohydrochloride, cheese amino acid, lipoids, gemmatein etc., also contain sodium phosphate, aluminium, magnesium, silicon
Acid, sulfate etc..Function clearing lung-heat, relieving sore-throat, antipyretic, hemostasis, cure mainly sore-throat, aphonia, pernio etc..Lasiosphaera nipponica(Kawam.)Y.Kobayasi is typically all to fry
Food can match sauerkraut, green pepper, wild leek and stir-fry and eat together.My family just matched local sour pickle and fried together today, the taste of Lasiosphaera nipponica(Kawam.)Y.Kobayasi
It is vinegar-pepper fresh and tender and with crisp sense.
It is the cultivation technique of Common puffball bacterium below：It includes tissue separation, the production of hybrid seeds, dispensing, inoculation, bacterium germination and fruiting pipe
Reason, cultural method carry out according to the following steps：First dispensing, that is, press formula as below and weight ratio weighs cotton benevolence powder 5-15%, wheat bran 2-
10%, sawdust 42-50%, fruit tree leaves 10-35%, cow dung 5-15%, potassium dihydrogen phosphate 2-5%, gypsum 1-2%, lime 1%,
Any of the above raw material is mixed thoroughly, water content is adjusted to 60-65%, compost stack after fermentation 25-30 days pack plastic bag cultivation immediately or
Furrow planting in mushroom shed seed furrow is inserted, bacterium germination, mushroom shed temperature are controlled at 16-26 DEG C in mushroom shed after inoculation, and illumination is dim light or the moon
Secretly, bed surface cleaning is carried out after the harvesting of the first damp mushroom, sprays a water, the second damp mushroom is harvested after 15-20 days, third and fourth tide
Mushroom management and so on, this method collects seed domestication to the time for about needing 1 year or so is harvested from Lasiosphaera nipponica(Kawam.)Y.Kobayasi, can be harvested per square meter
Lasiosphaera nipponica(Kawam.)Y.Kobayasi 2-3kg.The Cultivation method of above-listed artificial Lasiosphaera fenzlii strain pursues the high yield of Lasiosphaera nipponica(Kawam.)Y.Kobayasi unilaterally, has not regarded horse but
Mouthfeel, nutrition and the safety of vigorous strain.
Invention content
In order to be the edible strain that can manually cultivate by Lasiosphaera nipponica(Kawam.)Y.Kobayasi culture, a kind of culture of Lasiosphaera fenzlii strain of my invention
The Lasiosphaera fenzlii strain purity of method, this cultural method culture is high, and without miscellaneous bacteria, safety is good.
A kind of artificial culture method of Lasiosphaera fenzlii strain solves the problems, such as to adopt the technical scheme that by following step progress：
One, strain prepares：The Lasiosphaera fenzlii strain of wild maturation is acquired, the aleurioconidium body in thalline entity is extracted；Two, culture medium raw material is accurate
It is standby：40~50 parts of drying bagasse, 20~30 parts of dry clay, 15~20 parts of fine sand；Three, culture medium is modulated：It will dry sugarcane
Ground-slag is broken into the particle of diameter≤0.1cm, is blended into clay, fine sand, and being uniformly mixed becomes mixed culture material, greenhouse or indoor behaviour
Make；Four, culture medium goes out miscellaneous bacteria：Mixed culture material was heated to 70 DEG C~80 DEG C after one minute reduces temperature to 20 DEG C~30 naturally
℃；Five, culture medium forming is planted with strain：By after high-temperature sterilization mixed culture material be packed into plastic tub, loading depth 2cm~
3cm, surface sprinkle Lasiosphaera fenzlii strain conidia powder, spreading density：1g~1.5g/m2, then a thin layer mixed culture material is spread, slightly with tablet
It flattens, uniform dispersing dry defecates water to just moist, greenhouse or in-house operation；Six, overlay film moisture-heat preservation：Basal plane is cultivated with one
Thin transparent agricultural film covers, and surrounding is pushed down with soil block；Seven, the management during growth：Soil moisture content is kept to be not less than 16%,
It suitably sprays water when dry；Keep 25 DEG C~30 DEG C of canopy temperature as the temperature for urging the mushroom phase；Eight, the management during fruiting：Horse
Vigorous growth phase temperature, which is 25 DEG C~35 DEG C, to be advisable, and should open thin agricultural film, sunray within three days after Lasiosphaera fenzlii bacterial spawn fruiting phase
To increase Sunshade film when too strong, negative light degree and moist environment be kept, when the spherical diameter of fructification reaches 2.5~3.5cm
When will harvest in time, to keep fresh and tender palatable.
A kind of advantageous effect of the artificial culture method of Lasiosphaera fenzlii strain is：A kind of artificial culture method of Lasiosphaera fenzlii strain is only adopted
Bagasse and clay, fine sand is used to be handled as culture medium raw material, and by high-temp sterilizing, the miscellaneous bacteria fundamentally refused
In the presence of possible；A kind of cultural method of Lasiosphaera fenzlii strain does not take the raw material of other complexity such as excrement, chemical fertilizer to make culture medium,
So that Lasiosphaera fenzlii strain not only cannot be grown miscellaneous bacteria in growth course, Lasiosphaera fenzlii strain is also prevented to be evolved into toxic from absorbing complicated nutrient
The possibility of strain, bagasse sugar content and other nutritional ingredients are abundant enough, are safest culture medium, are edible mushroom
Kind considers that the present invention is safest edible bacterium cultural method as safest angle work.
Specific implementation mode
A kind of artificial culture method of Lasiosphaera fenzlii strain is carried out by following step：One, strain prepares：Acquire wild maturation
Lasiosphaera fenzlii strain, extract thalline entity in aleurioconidium body；Two, culture medium raw material prepares：40~50 parts of drying bagasse is done
20~30 parts of dry clay, 15~20 parts of fine sand；Three, culture medium is modulated：Drying bagasse is ground into the broken of diameter≤0.1cm
Grain, is blended into clay, fine sand, and being uniformly mixed becomes mixed culture material, greenhouse or in-house operation；Four, culture medium goes out miscellaneous bacteria：It will mix
Closing compost and being heated to 70 DEG C~80 DEG C after one minute reduces temperature to 20 DEG C~30 DEG C naturally；Five, culture medium forming and strain
Plantation：Mixed culture material after high-temperature sterilization is packed into plastic tub, loading depth 2cm~3cm, surface sprinkles Lasiosphaera fenzlii strain spore
Powder, spreading density：1g~1.5g/m2, then a thin layer mixed culture material is spread, it is slightly flattened with tablet, uniform dispersing dry defecates water
To just moist, greenhouse or in-house operation；Six, overlay film moisture-heat preservation：Culture basal plane is covered with a thin transparent agricultural film, surrounding soil
Block is pushed down；Seven, the management during growth：It keeps soil moisture content to be not less than 16%, suitably sprays water when dry；Keep greenhouse
25 DEG C~30 DEG C of interior temperature is as the temperature for urging the mushroom phase；Eight, the management during fruiting：Lasiosphaera fenzlii strain growth period temperature be 25 DEG C~
35 DEG C are advisable, and should open thin agricultural film within three days after Lasiosphaera fenzlii bacterial spawn fruiting phase, to increase Sunshade film when sunray is too strong, keep cloudy
Property light degree and moist environment, will be harvested in time when the spherical diameter of fructification reaches 2.5~3.5cm, to keep fresh and tender
It is palatable.
Claims (1)
1. a kind of artificial culture method of Lasiosphaera fenzlii strain, it is characterized in that being carried out by following step：One, strain prepares：Acquisition is wild
Ripe Lasiosphaera fenzlii strain is generated, the aleurioconidium body in thalline entity is extracted；Two, culture medium raw material prepares：Drying bagasse 40~
50 parts, 20~30 parts of dry clay, 15~20 parts of fine sand；Three, culture medium is modulated：By drying bagasse be ground into diameter≤
The particle of 0.1cm is blended into clay, fine sand, and being uniformly mixed becomes mixed culture material, greenhouse or in-house operation；Four, culture medium goes out
Miscellaneous bacteria：Mixed culture material was heated to 70 DEG C~80 DEG C after one minute reduces temperature to 20 DEG C~30 DEG C naturally；Five, culture medium at
Shape is planted with strain：Mixed culture material after high-temperature sterilization is packed into plastic tub, loading depth 2cm~3cm, surface sprinkles Lasiosphaera fenzlii
Strain conidia powder, spreading density：1g~1.5g/m2, then a thin layer mixed culture material is spread, it is slightly flattened with tablet, is uniformly sprayed
Clean clear water is to just moist, greenhouse or in-house operation；Six, overlay film moisture-heat preservation：Culture basal plane is covered with a thin transparent agricultural film,
Surrounding is pushed down with soil block；Seven, the management during growth：It keeps soil moisture content to be not less than 16%, suitably sprays water when dry；
Keep 25 DEG C~30 DEG C of canopy temperature as the temperature for urging the mushroom phase；Eight, the management during fruiting：Lasiosphaera fenzlii strain growth period temperature
It is advisable for 25 DEG C~35 DEG C, thin agricultural film should be opened within three days after Lasiosphaera fenzlii bacterial spawn fruiting phase, to increase sunshade when sunray is too strong
Film keeps negative light degree and moist environment, will be harvested in time when the spherical diameter of fructification reaches 2.5~3.5cm, with
Keep fresh and tender palatable.
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| CN201611067554.9A CN108370825A (en) | 2016-11-28 | 2016-11-28 | A kind of artificial culture method of Lasiosphaera fenzlii strain |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823702A (en) * | 2015-04-21 | 2015-08-12 | 吴中区胥口精益生物医药研究所 | Hericium erinaceus cultivation method |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823702A (en) * | 2015-04-21 | 2015-08-12 | 吴中区胥口精益生物医药研究所 | Hericium erinaceus cultivation method |
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Application publication date: 20180807 |
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