CN108323571A - A kind of wall material of probiotic microcapsule and the preparation method of probiotic microcapsule - Google Patents
A kind of wall material of probiotic microcapsule and the preparation method of probiotic microcapsule Download PDFInfo
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- CN108323571A CN108323571A CN201810129993.0A CN201810129993A CN108323571A CN 108323571 A CN108323571 A CN 108323571A CN 201810129993 A CN201810129993 A CN 201810129993A CN 108323571 A CN108323571 A CN 108323571A
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- 239000006041 probiotic Substances 0.000 title claims abstract description 81
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 81
- 239000003094 microcapsule Substances 0.000 title claims abstract description 77
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims abstract description 41
- 239000000463 material Substances 0.000 title claims abstract description 40
- 241000894006 Bacteria Species 0.000 claims abstract description 50
- 235000013325 dietary fiber Nutrition 0.000 claims abstract description 28
- 239000000725 suspension Substances 0.000 claims abstract description 19
- 150000002482 oligosaccharides Chemical class 0.000 claims abstract description 16
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 14
- 241000206575 Chondrus crispus Species 0.000 claims abstract description 14
- 239000000661 sodium alginate Substances 0.000 claims abstract description 14
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 14
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 14
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 11
- 235000013406 prebiotics Nutrition 0.000 claims abstract description 6
- 230000008901 benefit Effects 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 64
- 241000186000 Bifidobacterium Species 0.000 claims description 31
- 239000002775 capsule Substances 0.000 claims description 31
- 239000007788 liquid Substances 0.000 claims description 23
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 21
- 239000011162 core material Substances 0.000 claims description 19
- 230000004913 activation Effects 0.000 claims description 16
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- 235000015097 nutrients Nutrition 0.000 claims description 14
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- 238000002156 mixing Methods 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 11
- 230000004083 survival effect Effects 0.000 claims description 11
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- 239000008272 agar Substances 0.000 claims description 9
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 240000001046 Lactobacillus acidophilus Species 0.000 description 2
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- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 210000004051 gastric juice Anatomy 0.000 description 2
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- 238000003760 magnetic stirring Methods 0.000 description 2
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- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical class [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- 108010076119 Caseins Proteins 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
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- 108010010803 Gelatin Proteins 0.000 description 1
- 102000004407 Lactalbumin Human genes 0.000 description 1
- 108090000942 Lactalbumin Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
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- 102000057297 Pepsin A Human genes 0.000 description 1
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- 229920002472 Starch Polymers 0.000 description 1
- FRYDSOYOHWGSMD-UHFFFAOYSA-N [C].O Chemical compound [C].O FRYDSOYOHWGSMD-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
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- 230000001093 anti-cancer Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
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- 201000006549 dyspepsia Diseases 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000005428 food component Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000008821 health effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
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- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
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- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of preparation methods of the wall material of probiotic microcapsule, include the following steps：Sodium alginate, carragheen and dietary fiber are mixed, mixed with the sterile phosphate buffer of pH 6.8~7.4 after 15~30min of ultra violet lamp, the wall material solution that mass concentration is 8.5%~10.5% is made in 70~85 DEG C of water-bath dissolvings；In the wall material solution, the mass concentration of dietary fiber is 1%~5%；The mass concentration of sodium alginate is 2%~4%；The mass concentration of carragheen is 4.5%~6.5%.The invention also discloses the preparation method of probiotic microcapsule, the oligosaccharide solution (mass concentration 3%~5%) and prebiotic bacteria suspension (>=5 × 107CFU/mL volume ratio) is 1：2~2：1.The probiotic microcapsule of the present invention, embedding rate is high, has the advantages that the advantages of high viable count, high resistance to cold and diseases, easy to operate, economy and facility, suitable industrialized production.
Description
Technical field
The present invention relates to the micro- preparing technical field of probiotics, more particularly to the wall material of a kind of probiotic microcapsule and probiotics
The preparation method of microcapsules.
Background technology
Probiotics (Probiotics) is also known as probiotics, probiotic, be one kind by adjusting and improving the micro- life of enteron aisle
State balances, to play host the microorganism additive of beneficial effect.Probiotics is broadly divided into Bifidobacterium, lactobacillus
With gram-positive cocci category.Wherein, Bifidobacterium is a kind of Gram-positive bacillus, is the mankind and other warm-blooded animal enteron aisles
A kind of middle common probiotics.
Research finds that Bifidobacterium and many pathology of the mankind, physiological phenomenon are closely related, is the important mark of health
One of will has human body important physiology and healthcare function, including：1. having active anticancer；2. inhibiting saprophytic bacteria growth；3. preventing
Only constipation, reduces cholesterol, and prevention and treatment lactose digestion is bad；4. synthesizing vitamin and promoting the absorption of calcium.
The exploitation of Bifidobacterium Tiny ecosystem product is one of the hot spot studied both at home and abroad.The conduct pair of bifidobacteria viable bacteria product
The direct supplement of human body intestinal canal Bifidobacterium is more notable than taking bifidus factor preparation effect merely.But this kind of probiotics system
Product number of viable requires more than 106CFU/g or 106CFU/mL can be only achieved the effect of health care.However, in production, storage, transport etc.
In the process, probiotics will by food component (acid, oxygen, additive etc.), storage temperature and Host Digestion system (hydrochloric acid in gastric juice,
Cholate, enzyme) etc. influence, often result in viable count and decline to a great extent.In Australia and Europe, expert is by measuring different brands
The survival rate of lactobacillus acidophilus and Bifidobacterium in Yoghourt finds that the content of these probiotics in most Yoghourts is all very low, especially
It is Bifidobacterium.
Freeze-drying, microcapsules technology and technology for coating can be used to solve this problem at present.But, freeze-drying system
That there are costs is higher for standby bifidobacteria viable bacteria solid pharmaceutical preparation, and the operating time is long, and production efficiency is relatively low, and the preservation term of bacterium powder is shorter etc.
Defect；The shortcomings of technology for coating presence is not easy to operate, and thicknesses of layers is difficult to control.Probiotics is wrapped using microcapsules technology
Bury the effective ways having become in order to solve the above problem.
Microcapsules technology refer to by the small substance such as solid, gas, liquid, using natural or synthetic high molecular material into
Row coating obtains the technology of fine particle.As one of 21 century new and high technology, main function is to concentrate microcapsules technology
Particle or liquid achieve the effect that protection, deliver and delay release.
For microcapsule product, the selection of wall material plays decisive role to the physicochemical property of product.It is prebiotic for embedding
The wall material of bacterium is mostly natural macromolecular material.Currently, the wall material of probiotic microcapsule is in the majority with carbohydrate and protein, carbon
Hydrate species have starch, maltodextrin, chitosan etc.；Protein has lactalbumin, casein, gelatin etc..But wall material is difficult to
Selection had especially not only had enteric solubility but also to the wall material of no damage to human body than sparser.
Invention content
In order to overcome the disadvantages mentioned above and deficiency of the prior art, the purpose of the present invention is to provide a kind of probiotic microcapsules
Wall material, substantially increase the embedding yield of probiotic microcapsule.
Another object of the present invention is to provide the preparation methods of above-mentioned probiotic microcapsule.
The purpose of the present invention is achieved through the following technical solutions：
A kind of preparation method of the wall material of probiotic microcapsule, includes the following steps：
Sodium alginate, carragheen and dietary fiber are mixed, with pH's 6.8~7.4 after 15~30min of ultra violet lamp
Sterile phosphate buffer mixes, and the wall material solution that mass concentration is 8.5%~10.5% is made in 70~85 DEG C of water-bath dissolvings；Institute
It states in wall material solution, the mass concentration of dietary fiber is 1%~5%；The mass concentration of sodium alginate is 2%~4%；Carragheen
Mass concentration be 4.5%~6.5%.
A kind of preparation method of probiotic microcapsule, includes the following steps：
(1) in the wall material for the probiotic microcapsule that claim 1 is prepared, core material solution, the volume of the two is added
Than being 1：2~2：1, it is uniformly mixed；The core material solution includes oligosaccharide solution and prebiotic bacteria suspension, obtains mixed liquor；
(2) mixed liquor for being obtained step (1) with the injection of microcapsules generator is expressed to and is placed in a manner of instilling dropwise
3%~5%CaCl of mass concentration in magnetic stirring apparatus2In solution, 30~33min is continuously stirred, forms capsule；
(3) wet capsule is obtained with the normal saline flushing for bacterium of having gone out again；
(4) wet capsule is placed in -80 DEG C of low temperature refrigerator after 2~4h of pre-freeze, is placed in vacuum freeze drier, -80
DEG C freeze-drying 24~48h, be made microscapsule powder
The preparation method of step (1) the prebiotic bacteria suspension, specially：
The seed that the probiotics glycerol tube being stored under the conditions of -20 DEG C preserves is inoculated in 121 ± 1 DEG C of sterilizings 15 by (1-1)
In~20min MRS fluid nutrient mediums after cooling, under the conditions of 36 ± 1 DEG C, probiotics 24~36h of stationary culture is carried out first
Activation；
The probiotic's culture liquid of primary activation is accessed the training of MRS liquid by the ratio that (1-2) is 1%~5% in mass concentration
It supports and is activated in base, under the conditions of 36 ± 1 DEG C, culture 18~for 24 hours, obtain probiotic bacterial cultures；
The probiotic bacterial cultures that step (1-2) obtains are centrifuged off supernatant by (1-3), are collected bacterium mud, are added and train
0.9% isometric sterile saline of nutrient solution, concussion are spare after mixing；Then use MRS agar mediums in temperature
48~72h of culture is poured at 36 ± 1 DEG C.
Step (1-1) described centrifugation, specially：30~33min is stirred with the rotating speed of 4000~4200r/min.
Step (2) magnetic stirring apparatus is stirred with the rotating speed of 100~200r/min.
The mass concentration of the oligosaccharide solution is 3%~5%.
The oligosaccharide solution and the volume ratio of prebiotic bacteria suspension are 1：2~2：1.
The probiotics includes bifidobacterium longum, bifidobacterium breve, bifidobacterium adolescentis, bifidobacterium bifidum, animal pair
At least one of discrimination bacillus；The dietary fiber is banana fiber.
The probiotic microcapsule that the preparation method of the probiotic microcapsule is prepared, embedding rate >=70%, viable count
It is 106~107CFU/g, after freeze-dried, survival rate is 30%~50%.
Compared with prior art, the present invention has the following advantages and beneficial effect：
(1) wall material of the invention is substantially increased embedding yield, is made using dietary fiber, sodium alginate, carragheen combination
Probiotics quantity in unit mass microcapsules is more than 107CFU, guarantee have sufficient amount of probiotics to survive in human body, send out
Wave its healthcare function.
(2) dietary fiber, sodium alginate, the carragheen of wall material of the invention use are all natural large biological molecules, raw
Object compatibility is good, has no toxic side effect；Meanwhile multi-section divides indigestion and enters in enteron aisle dietary fiber under one's belt, sodium alginate can
To improve tolerance of the probiotics to pepsin etc., carragheen can increase system viscosity, gel strength and elasticity, three
In conjunction with the viable count that can be greatly improved into enteron aisle.
(3) probiotic microcapsule stomach juice-resistant ability of the invention is good；Release performance is good in enteric liquid, in simulating enteric liquid
Disintegration is complete substantially by 90min；Anti- vacuum refrigeration is good, and after freeze-dried, survival rate is higher, up to 50%.
(4) present invention is embedded probiotics by microcapsules technology, the capacity antacid of probiotics itself is improved, to prolong
The room temperature storage life for having grown viable bacteria, improves viable bacteria ordinary temperature stability.
(5) present invention is added to freeze drying protectant oligosaccharide, and to improve tolerance of the probiotics to environment, it is dry to reduce freezing
The loss of probiotics in dry and transport storage can obtain the high microcapsule life tonifying bacterium of freeze-drying survival rate, meanwhile, it is functional oligomeric
The product that sugar and probiotic combinations are formed is while playing each self-applying, moreover it is possible to play synergistic effect.When animal takes in function
Property product of the oligosaccharide as probiotics freeze drying protectant after, functional oligose, which will continue to play it, promotes growth of probiotics
Effect so that probiotics reinforces the regulating power of environment, to reduce probiotics in producing and processing and transporting storage
Loss, make its play the role of diseases prevention, it is disease-resistant and improve animal body immune function.
(6) oligosaccharide of the invention, dietary fiber sheet have together as benefit materials and probiotics promotes health
Effect.
(7) present invention can apply in the food such as Yoghourt, milk, yogurt.
Description of the drawings
Fig. 1 is the process flow chart of the present invention.
Fig. 2 is probiotic microcapsule scanning electron microscope (SEM) (× 200) figure of the present invention.
Fig. 3 is probiotic microcapsule scanning electron microscope (SEM) (× 2000) figure of the present invention
Fig. 4 is probiotic microcapsule intestinal juice release test result figure of the present invention.
Specific implementation mode
With reference to embodiment, the present invention is described in further detail, embodiments of the present invention are not limited thereto.
Embodiment 1
As shown in Figure 1, the bifid bacterium microcapsule of a kind of the addition oligosaccharide core material and dietary fiber wall material of the present embodiment
Preparation method, include the following steps：
(1) activation of bacterial strain
The seed that the Bifidobacterium glycerol tube being stored under the conditions of -20 DEG C preserves is inoculated in 121 DEG C of sterilizing 15min coolings
In MRS fluid nutrient mediums afterwards, under the conditions of 37 DEG C, stationary culture 36h obtains activated seed liquid.
The Bifidobacterium of the present embodiment is animal bifidobacteria BB-12, purchased from the gloomy company of red macat.
(2) expand culture
It will be living in the Bifidobacterium culture solution access MRS fluid nutrient mediums of primary activation in the ratio of 3% (mass concentration)
Change, under the conditions of 37 DEG C, culture for 24 hours, obtains bifidobacterium culture.
(3) bacteria suspension is prepared
The bifidobacterium species centrifugation (4100r/min, 10min) activated to the third generation is removed into supernatant, collects bacterium
Mud adds 0.9% sterile saline isometric with culture solution, firmly shakes, spare after mixing.Then it uses
MRS agar mediums pour into culture 48h at 37 DEG C of temperature, through count to get pour into the viable bacteria strain density of culture solution be 5 ×
107CFU/mL or more；
(4) preparation of wall material
Claim dietary fiber 0.3g, sodium alginate 0.15g, carragheen 0.275g, mixing, after ultra violet lamp 30min with 5mL
The sterile phosphate buffer of pH 6.8 mixes, 75 DEG C of water-bath dissolvings.
The dietary fiber of the present embodiment is extracted from banana skin, and extraction step is as follows：
After banana skin vacuum freeze drying, is crushed using pulverizer and be sieved (40 mesh).Banana peel dry powder is weighed to be put into
In bottle with cover, 12% NaOH solution, solid-liquid ratio 2g is added：50mL.Bottle is put into and is cleaned by ultrasonic ultrasound in instrument
30min, after be put into water-bath 90min in 61 DEG C of water-bath.After taking-up, the adding citric acid tune pH to 7.0 or so in blue lid bottle.It is blue
Liquid in lid bottle pours into centrifuge tube, and centrifuge tube is put into centrifuge (5000r/min, 6min) and carries out centrifugal treating.It filters out
Filtrate filters (being rinsed with water) after absolute ethyl alcohol decoloration 3h is added in filter residue, takes out filter residue, be placed in tinfoil, be put into electricity
In hot blast drying box dry (4~5h), until constant weight.
(5) preparation of core material
By 3% oligofructose solution and Bifidobacterium bacteria suspension with 1：The ratio of 1 (volume) mixes.
(6) preparation of microcapsules
1. taking the wall material solution 5mL prepared, isometric core material solution is added, is uniformly mixed.
2. being sprayed above-mentioned uniformly mixed solution in a manner of instilling dropwise with microcapsules generator, it is expressed to and is placed in magnetic
Mass concentration 5%CaCl on power blender (100r/min, 37 DEG C)2In solution, 33min is continuously stirred, forms capsule.
3. the wet capsule of formation is filtered with filter paper afterwards.
4. wet capsule is rinsed 3 times with the physiological saline for bacterium of having gone out again.
(7) it is freeze-dried
Wet capsule is placed in -80 DEG C of low temperature refrigerator after pre-freeze 2h, is placed in vacuum freeze drier, -80 DEG C of freezings
For 24 hours, microscapsule powder is made in drying.
Performance measurement as described below has been carried out using the probiotic microcapsule that the method for the present embodiment is prepared.
(1) embedding yield measures
The wet capsules of 1g are weighed in beaker, the solution cyst fluid that 9mL pH value is 7.4 is added, 37 DEG C vibrate up to disintegration completely,
Bacteria suspension is made, for inoculation.
By sample to be tested sterile saline with 10 times of gradient dilutions, it is properly dilute to draw 0.1mL with the sterile liquid-transfering guns of 1mL
The bacteria suspension of degree of releasing (viable count is within the scope of 30~300CFU/mL) is dripped on tablet, is smoothened with spreader, is numbered.Wait for agar
After solidification, by Flat plate turnover, 36 ± 1 DEG C of 48 ± 2h of Anaerobic culturel can extend to 72 ± 2h.It is counted after culture all on tablet
Clump count.Converse the viable count of bifid bacterium microcapsule unit volume.
Viable count calculation formula：Viable count (the CFU/mL)=repetition solid medium bacterium colony average of same dilution 3
× extension rate.
The calculation formula of the wet capsule viable counts of 1g is after opening one's purse：N1=G1×V1
Embed yield (%)=N1×M1/(N0×V0) × 100%
In formula：
G1- 1mL solves the viable count (CFU/mL) in cyst fluid；
V1The volume (mL) of-solution cyst fluid；
N1- open one's purse after capsule viable count (CFU/g)；
N0Original bacteria liquid viable count (CFU/mL) before-embedding；
V0- prepare the volume (mL) of original bacteria liquid used in microcapsules；
M1The total weight (g) of-gained capsule.
As seen from the above table, the embedding yield of the present invention can be improved by being added to dietary fiber and oligofructose.
(2) somatometry of physique
Probiotic microcapsule ultra micro knot of the present invention is observed under the conditions of conventional study using scanning electron microscope (SEM)
Structure.The probiotic microcapsule after freeze-drying is sticked on sample stage with double faced adhesive tape, scanning electron microscopic observation microcapsules are used after metal spraying
Surface texture, the result is shown in Fig. 2.This result shows that probiotic microcapsule compact structure of the present invention, surface it is smooth, it is internal be in bee
Nest shape structure.
(3) stomach juice-resistant is tested
Microcapsules 1g of the present invention (freeze-drying microcapsules addition is 0.1g) is weighed, manual simulation's stomach of 9mL is scattered in
It in liquid, is stored in 37 DEG C of constant temperature, 1 pipe is taken out after 0,1 and 2h, with 0.9% normal saline flushing, prepared solution is added
It opens one's purse in cyst fluid, wherein the volume of solution cyst fluid is 9mL, 1mL is therefrom pipetted after the completion of opening one's purse and carries out gradient dilution, is used
MRS agar mediums carry out count plate.As a contrast with the bacterium solution that does not embed.The results are shown in following table.These result tables
Bright, viable count fall of the probiotics of the present invention in simulate the gastric juice is smaller, and the viable count of the probiotics subtracts when keeping the temperature 2h
Few 1.28 × 106CFU/g。
(4) intestinal juice release property is tested
Weigh wet microcapsules sample lg (freeze-drying microcapsules addition be 0.1g), be placed in 9mL simulated intestinal fluids, in 37 DEG C,
150~210r/min shaken cultivations.1 pipe is taken out respectively at 0,30,60,90,120,150min, simulated intestinal fluid is directly drawn, surveys
Its fixed light transmittance at 600nm wavelength.The results are shown in Fig. 3.These results indicate that microcapsules are in simulating enteric liquid
Disintegration is complete substantially by 90min.
(5) anti-vacuum refrigeration test
It weighs and is added in 9mL solution cyst fluids in the front and back probiotic microcapsule 0.1g of the present invention of freeze-drying respectively, in temperature 37
Shake culture 40min at DEG C discharges thalline completely, then carries out bacterium colony counting.
The calculation formula of 1g freeze-drying capsules viable count is after opening one's purse：N2=G2×V2/0.1
Survival rate (%)=N is lyophilized2×M2/(N0×V0×M1) × 100%
In formula：
G2- 1mL solves the viable count (CFU/mL) in cyst fluid；
V2The volume (mL) of-solution cyst fluid；
N2Product after-drying open one's purse after viable count (CFU/g)；
M2Product quality (g) after-drying；
M1Product quality (g) before-drying；
N0- the viable count (CFU/mL) being added；
V0- prepare the volume (mL) of original bacteria liquid used in microcapsules；
The result shows that after the microcapsules for being added to dietary fiber and oligosaccharide are freeze-dried, survival rate is higher, reachable
30%~50%.
Embodiment 2
A kind of preparation method of the addition oligosaccharide core material of the present embodiment and the bifid bacterium microcapsule of dietary fiber wall material,
Include the following steps：
(1) activation of bacterial strain
The seed that the Bifidobacterium glycerol tube being stored under the conditions of -20 DEG C preserves is inoculated in 122 DEG C of sterilizing 20min coolings
In MRS fluid nutrient mediums afterwards, under the conditions of 35 DEG C, stationary culture 36h obtains activated seed liquid.
The Bifidobacterium of the present embodiment is animal bifidobacteria BB-12, purchased from the gloomy company of red macat.
(2) expand culture
It will be living in the Bifidobacterium culture solution access MRS fluid nutrient mediums of primary activation in the ratio of 5% (mass concentration)
Change, under the conditions of 35 DEG C, culture for 24 hours, obtains bifidobacterium culture.
(3) bacteria suspension is prepared
The bifidobacterium species centrifugation (4000r/min, 30min) activated to the third generation is removed into supernatant, collects bacterium
Mud adds 0.9% sterile saline isometric with culture solution, firmly shakes, spare after mixing.Then it uses
MRS agar mediums pour into culture 48h at 37 DEG C of temperature, through count to get pour into the viable bacteria strain density of culture solution be 5 ×
107CFU/mL or more.
(4) preparation of wall material
Claim dietary fiber 0.10g, sodium alginate 0.10g, carragheen 0.225g, mixing, after ultra violet lamp 30min with
The sterile phosphate buffer of 5mL pH 6.8 mixes, 75 DEG C of water-bath dissolvings.
The dietary fiber of the present embodiment is extracted from banana skin, and extraction step is as follows：
After banana skin vacuum freeze drying, is crushed using pulverizer and be sieved (40 mesh).Banana peel dry powder is weighed to be put into
In bottle with cover, 12% NaOH solution, solid-liquid ratio 2g is added：50mL.Bottle is put into and is cleaned by ultrasonic ultrasound in instrument
30min, after be put into water-bath 90min in 61 DEG C of water-bath.After taking-up, the adding citric acid tune pH to 7.0 or so in blue lid bottle.It is blue
Liquid in lid bottle pours into centrifuge tube, and centrifuge tube is put into centrifuge (5000r/min, 6min) and carries out centrifugal treating.It filters out
Filtrate filters (being rinsed with water) after absolute ethyl alcohol decoloration 3h is added in filter residue, takes out filter residue, be placed in tinfoil, be put into electricity
In hot blast drying box dry (4~5h), until constant weight.
(5) preparation of core material
By 5% oligofructose solution and Bifidobacterium bacteria suspension with 2：The ratio of 1 (volume) mixes.
(6) preparation of microcapsules
1. taking the wall material solution 5mL prepared, the core material solution of 2 times of volumes is added, is uniformly mixed.
2. being sprayed above-mentioned uniformly mixed solution in a manner of instilling dropwise with microcapsules generator, it is expressed to and is placed in magnetic
Mass concentration 5%CaCl on power blender (100r/min, 37 DEG C)2In solution, 30min is continuously stirred, forms capsule.
3. the wet capsule of formation is filtered with filter paper afterwards.
4. wet capsule is rinsed 3 times with the physiological saline for bacterium of having gone out again.
(7) it is freeze-dried
Wet capsule is placed in -80 DEG C of low temperature refrigerator after pre-freeze 2h, is placed in vacuum freeze drier, -80 DEG C of freezings
For 24 hours, microscapsule powder is made in drying.
The microcapsules test result that the present embodiment is prepared is similar to Example 1, probiotics survival rate up to 45% with
On.
Embodiment 3
A kind of preparation method of the addition oligosaccharide core material of the present embodiment and the bifid bacterium microcapsule of dietary fiber wall material,
Include the following steps：
(1) activation of bacterial strain
The seed that the Bifidobacterium glycerol tube being stored under the conditions of -20 DEG C preserves is inoculated in 120 DEG C of sterilizing 15min coolings
In MRS fluid nutrient mediums afterwards, under the conditions of 36 DEG C, stationary culture 36h obtains activated seed liquid.
The Bifidobacterium of the present embodiment is animal bifidobacteria BB-12, purchased from the gloomy company of red macat.
(2) expand culture
It will be living in the Bifidobacterium culture solution access MRS fluid nutrient mediums of primary activation in the ratio of 1% (mass concentration)
Change, under the conditions of 36 DEG C, culture for 24 hours, obtains bifidobacterium culture.
(3) bacteria suspension is prepared
The bifidobacterium species centrifugation (4000r/min, 30min) activated to the third generation is removed into supernatant, collects bacterium
Mud adds 0.9% sterile saline isometric with culture solution, firmly shakes, spare after mixing.Then it uses
MRS agar mediums pour into culture 48h at 37 DEG C of temperature, through count to get pour into the viable bacteria strain density of culture solution be 5 ×
107CFU/mL or more；
(4) preparation of wall material
Claim dietary fiber 0.50g, sodium alginate 0.20g, carragheen 0.325g, mixing, after ultra violet lamp 30min with
The sterile phosphate buffer of 5mL pH 6.8 mixes, 75 DEG C of water-bath dissolvings.
The dietary fiber of the present embodiment is extracted from banana skin, and extraction step is as follows：
After banana skin vacuum freeze drying, is crushed using pulverizer and be sieved (40 mesh).Banana peel dry powder is weighed to be put into
In bottle with cover, 12% NaOH solution, solid-liquid ratio 2g is added：50mL.Bottle is put into and is cleaned by ultrasonic ultrasound in instrument
30min, after be put into water-bath 90min in 61 DEG C of water-bath.After taking-up, the adding citric acid tune pH to 7.0 or so in blue lid bottle.It is blue
Liquid in lid bottle pours into centrifuge tube, and centrifuge tube is put into centrifuge (5000r/min, 6min) and carries out centrifugal treating.It filters out
Filtrate filters (being rinsed with water) after absolute ethyl alcohol decoloration 3h is added in filter residue, takes out filter residue, be placed in tinfoil, be put into electricity
In hot blast drying box dry (4~5h), until constant weight.
(5) preparation of core material
By 3% oligofructose solution and Bifidobacterium bacteria suspension with 1：The ratio of 2 (volumes) mixes.
(6) preparation of microcapsules
1. taking the wall material solution 5mL prepared, the core material solution of half volume is added, is uniformly mixed.
2. being sprayed above-mentioned uniformly mixed solution in a manner of instilling dropwise with microcapsules generator, it is expressed to and is placed in magnetic
Mass concentration 3%CaCl on power blender (100r/min, 37 DEG C)2In solution, 33min is continuously stirred, forms capsule.
3. the wet capsule of formation is filtered with filter paper afterwards.
4. wet capsule is rinsed 3 times with the physiological saline for bacterium of having gone out again.
(7) it is freeze-dried
Wet capsule is placed in -80 DEG C of low temperature refrigerator after pre-freeze 4h, is placed in vacuum freeze drier, -80 DEG C of freezings
Dry 48h, is made microscapsule powder.
The microcapsules test result that the present embodiment is prepared is similar to Example 1, probiotics survival rate up to 40% with
On.
Embodiment 4
A kind of preparation side of the addition oligosaccharide core material of the present embodiment and the bifidobacterium longum microcapsules of dietary fiber wall material
Method includes the following steps：
(1) activation of bacterial strain
It is cold that the seed that the bifidobacterium longum glycerol tube being stored under the conditions of -20 DEG C preserves is inoculated in 120 DEG C of sterilizing 15min
But in the MRS fluid nutrient mediums after, under the conditions of 37 DEG C, stationary culture 36h obtains activated seed liquid.
The bifidobacterium longum of the present embodiment is purchased from China General Microbiological culture presevation administrative center.
(2) expand culture
It will be in the bifidobacterium longum culture solution access MRS fluid nutrient mediums of primary activation in the ratio of 1% (mass concentration)
Activation, under the conditions of 37 DEG C, culture for 24 hours, obtains bifidobacterium longum culture.
(3) bacteria suspension is prepared
The strain centrifugation (4000r/min, 30min) activated to the third generation is removed into supernatant, bacterium mud is collected, adds
0.9% isometric sterile saline, firmly shakes with culture solution, spare after mixing.Then MRS agar cultures are used
Base pours into culture 48h at 37 DEG C of temperature, and through counting to get, to pour into the viable bacteria strain density of culture solution be 5 × 107CFU/mL or more；
(4) preparation of wall material
Claim dietary fiber 0.3g, sodium alginate 0.15g, carragheen 0.275g, mixing, after ultra violet lamp 30min with 5mL
The sterile phosphate buffer of pH 6.8 mixes, 75 DEG C of water-bath dissolvings.
The dietary fiber of the present embodiment is extracted from banana skin, and extraction step is same as Example 1, and details are not described herein.
(5) preparation of core material
By 3% xylo-oligosaccharide solution and lactobacillus acidophilus bacteria suspension with 1：The ratio of 2 (volumes) mixes.
(6) preparation of microcapsules
1. taking the wall material solution 5mL prepared, the core material solution of half volume is added, is uniformly mixed.
2. being sprayed above-mentioned uniformly mixed solution in a manner of instilling dropwise with microcapsules generator, it is expressed to and is placed in magnetic
Mass concentration 3%CaCl on power blender (100r/min, 37 DEG C)2In solution, 33min is continuously stirred, forms capsule.
3. the wet capsule of formation is filtered with filter paper afterwards.
4. wet capsule is rinsed 3 times with the physiological saline for bacterium of having gone out again.
(7) it is freeze-dried
Wet capsule is placed in -80 DEG C of low temperature refrigerator after pre-freeze 4h, is placed in vacuum freeze drier, -80 DEG C of freezings
Dry 48h, is made microscapsule powder.
The microcapsules test result that the present embodiment is prepared is similar to Example 1, probiotics survival rate up to 32% with
On.
Embodiment 5
A kind of preparation side of the addition oligosaccharide core material of the present embodiment and the bifidobacterium breve microcapsules of dietary fiber wall material
Method includes the following steps：
(1) activation of bacterial strain
It is cold that the seed that the bifidobacterium breve glycerol tube being stored under the conditions of -20 DEG C preserves is inoculated in 120 DEG C of sterilizing 15min
But in the MRS fluid nutrient mediums after, under the conditions of 37 DEG C, stationary culture 36h obtains activated seed liquid.
(2) expand culture
It will be in the bifidobacterium breve culture solution access MRS fluid nutrient mediums of primary activation in the ratio of 1% (mass concentration)
Activation, under the conditions of 37 DEG C, culture for 24 hours, obtains bifidobacterium breve culture.
(3) bacteria suspension is prepared
The strain centrifugation (4000r/min, 30min) activated to the third generation is removed into supernatant, bacterium mud is collected, adds
0.9% isometric sterile saline, firmly shakes with culture solution, spare after mixing.Then MRS agar cultures are used
Base pours into culture 48h at 37 DEG C of temperature, and through counting to get, to pour into the viable bacteria strain density of culture solution be 5 × 107CFU/mL or more；
(4) preparation of wall material
Claim dietary fiber 0.3g, sodium alginate 0.15g, carragheen 0.275g, mixing, after ultra violet lamp 30min with 5mL
The sterile phosphate buffer of pH 6.8 mixes, 75 DEG C of water-bath dissolvings.
The dietary fiber of the present embodiment is extracted from banana skin, and extraction step is same as Example 1, and details are not described herein.
(5) preparation of core material
By 3% xylo-oligosaccharide solution and bifidobacterium breve bacteria suspension with 1：The ratio of 2 (volumes) mixes.
(6) preparation of microcapsules
1. taking the wall material solution 5mL prepared, the core material solution of half volume is added, is uniformly mixed.
2. being sprayed above-mentioned uniformly mixed solution in a manner of instilling dropwise with microcapsules generator, it is expressed to and is placed in magnetic
Mass concentration 3%CaCl on power blender (100r/min, 37 DEG C)2In solution, 33min is continuously stirred, forms capsule.
3. the wet capsule of formation is filtered with filter paper afterwards.
4. wet capsule is rinsed 3 times with the physiological saline for bacterium of having gone out again.
(7) it is freeze-dried
Wet capsule is placed in -80 DEG C of low temperature refrigerator after pre-freeze 4h, is placed in vacuum freeze drier, -80 DEG C of freezings
Dry 48h, is made microscapsule powder.
The microcapsules test result that the present embodiment is prepared is similar to Example 1, probiotics survival rate up to 48% with
On.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by the embodiment
Limitation, it is other it is any without departing from the spirit and principles of the present invention made by changes, modifications, substitutions, combinations, simplifications,
Equivalent substitute mode is should be, is included within the scope of the present invention.
Claims (9)
1. a kind of preparation method of the wall material of probiotic microcapsule, which is characterized in that include the following steps：
Sodium alginate, carragheen and dietary fiber are mixed, the sterile phosphorus after 15~30min of ultra violet lamp with pH6.8~7.4
Acid buffer mixes, and the wall material solution that mass concentration is 8.5%~10.5% is made in 70~85 DEG C of water-bath dissolvings；The wall material
In solution, the mass concentration of dietary fiber is 1%~5%；The mass concentration of sodium alginate is 2%~4%；The quality of carragheen
A concentration of 4.5%~6.5%.
2. a kind of preparation method of probiotic microcapsule, which is characterized in that include the following steps：
(1) in the wall material for the probiotic microcapsule that claim 1 is prepared, core material solution is added, the volume ratio of the two is
1：2~2：1, it is uniformly mixed；The core material solution includes oligosaccharide solution and prebiotic bacteria suspension, obtains mixed liquor；
(2) mixed liquor for being obtained step (1) with the injection of microcapsules generator is expressed in a manner of instilling dropwise and is placed in magnetic force
3%~5%CaCl of mass concentration in blender2In solution, 30~33min is continuously stirred, forms capsule；
(3) wet capsule is obtained with the normal saline flushing for bacterium of having gone out again；
(4) wet capsule is placed in -80 DEG C of low temperature refrigerator after 2~4h of pre-freeze, is placed in vacuum freeze drier, -80 DEG C cold
Dry 24~48h is lyophilized, microscapsule powder is made.
3. the preparation method of probiotic microcapsule according to claim 1, which is characterized in that step (1) described probiotics
The preparation method of suspension, specially：
(1-1) will be stored in the seed that probiotics glycerol tube under the conditions of -20 DEG C preserves be inoculated in 121 ± 1 DEG C of sterilizings 15~
In 20min MRS fluid nutrient mediums after cooling, under the conditions of 36 ± 1 DEG C, probiotics 24~36h of stationary culture is lived for the first time
Change；
The probiotic's culture liquid of primary activation is accessed MRS fluid nutrient mediums by (1-2) in the ratio that mass concentration is 1%~5%
Middle activation, under the conditions of 36 ± 1 DEG C, culture 18~for 24 hours, obtain probiotic bacterial cultures；
The probiotic bacterial cultures that step (1-2) obtains are centrifuged off supernatant by (1-3), collect bacterium mud, are added and culture solution
0.9% isometric sterile saline, concussion are spare after mixing；Then use MRS agar mediums temperature 36 ±
48~72h of culture is poured at 1 DEG C.
4. the preparation method of probiotic microcapsule according to claim 1, which is characterized in that step (1-1) described centrifugation,
Specially：10min is stirred with the rotating speed of 4000~4200r/min.
5. the preparation method of probiotic microcapsule according to claim 1, which is characterized in that step (2) described magnetic force stirs
Device is mixed to stir with the rotating speed of 100~200r/min.
6. the preparation method of probiotic microcapsule according to claim 1, which is characterized in that the matter of the oligosaccharide solution
Measure a concentration of 3%~5%.
7. the preparation method of probiotic microcapsule according to claim 1, which is characterized in that the oligosaccharide solution and benefit
The volume ratio of raw bacteria suspension is 1：2~2：1.
8. the preparation method of probiotic microcapsule according to claim 1, which is characterized in that the probiotics includes long double
At least one of discrimination bacillus, bifidobacterium breve, bifidobacterium adolescentis, bifidobacterium bifidum, animal bifidobacteria；The diet
Fiber is banana fiber.
9. the probiotic microcapsule that the preparation method of any one of claim 2~8 probiotic microcapsule is prepared, special
Sign is, embedding rate >=70%, viable count 106~107CFU/g, after freeze-dried, survival rate is 30%~50%.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109527563A (en) * | 2018-12-24 | 2019-03-29 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of synbiotics microcapsule and its preparation method and application |
| CN109619593A (en) * | 2018-11-08 | 2019-04-16 | 淮阴工学院 | A kind of probiotic double layer microcapsules and preparation method thereof |
| CN109717481A (en) * | 2018-12-27 | 2019-05-07 | 广州智特奇生物科技股份有限公司 | A kind of preparation process of coating probiotics |
| CN109864137A (en) * | 2019-03-15 | 2019-06-11 | 华中农业大学 | A kind of quick-fried pearl Yoghourt and preparation method thereof |
| CN110771906A (en) * | 2019-11-29 | 2020-02-11 | 高州市青湖农业发展有限公司 | Preparation method of banana peel water-insoluble dietary fiber |
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| CN109527563A (en) * | 2018-12-24 | 2019-03-29 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of synbiotics microcapsule and its preparation method and application |
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| CN111436614A (en) * | 2020-04-28 | 2020-07-24 | 天津大学 | Probiotics delivery microcapsule based on mushroom soluble dietary fiber and preparation method |
| CN111616231A (en) * | 2020-07-16 | 2020-09-04 | 成都大学 | Preparation method of synbiotics whole bean curd |
| CN112206242A (en) * | 2020-08-05 | 2021-01-12 | 内蒙古农业大学 | Fungus microcapsule for killing animal parasite eggs and preparation method and application thereof |
| CN113317516A (en) * | 2021-05-12 | 2021-08-31 | 华南理工大学 | Soybean dietary fiber weight-losing meal-replacing biscuit based on 3D printing and preparation method thereof |
| CN113854361A (en) * | 2021-10-08 | 2021-12-31 | 福建技术师范学院 | Freeze-dried strawberry probiotic milk bean with core-shell structure and preparation method thereof |
| CN113854361B (en) * | 2021-10-08 | 2023-12-22 | 福建技术师范学院 | Freeze-dried strawberry probiotic milk beans with core-shell structure and preparation method thereof |
| CN114711435B (en) * | 2022-04-06 | 2023-05-09 | 深圳安馨堂生物科技有限公司 | Dietary fiber probiotic composition and preparation method thereof |
| CN115606811A (en) * | 2022-04-19 | 2023-01-17 | 中国科学院烟台海岸带研究所 | Preparation method of stichopus japonicus visceral oligopeptide microcapsules and microcapsules |
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