CN108310798A - A kind of solid phase microextraction pre-treatment component and solid-phase micro-extracting device - Google Patents
A kind of solid phase microextraction pre-treatment component and solid-phase micro-extracting device Download PDFInfo
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- CN108310798A CN108310798A CN201810296226.9A CN201810296226A CN108310798A CN 108310798 A CN108310798 A CN 108310798A CN 201810296226 A CN201810296226 A CN 201810296226A CN 108310798 A CN108310798 A CN 108310798A
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- extraction flask
- bottle
- extraction
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- flask
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0215—Solid material in other stationary receptacles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4055—Concentrating samples by solubility techniques
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/046—Function or devices integrated in the closure
- B01L2300/047—Additional chamber, reservoir
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4055—Concentrating samples by solubility techniques
- G01N2001/4061—Solvent extraction
Abstract
The present invention relates to a kind of solid phase microextraction pre-treatment component and solid-phase micro-extracting devices.Solid-phase micro-extracting device includes extraction needle and extraction flask, extraction flask bottle mouth position is equipped with for extraction needle hermetically passing and is inserted into the sealing structure in extraction flask, the device further includes the squeeze bottle with extraction flask in axial direction air-tight movable cooperation, the inner cavity of squeeze bottle constitutes the accommodation space for placing sample and can coordinate with the bottom of bottle of extraction flask when squeeze bottle is moved close to extraction flask and racks sample, and the bottom of bottle of extraction flask is additionally provided with the channel that the object to be extracted in the sample for Gong being squeezed and broken enters extraction flask.Sample is placed in squeeze bottle, squeeze bottle is set to be moved close to extraction flask until sample is squeezed and broken, object to be extracted in sample is entered by extraction flask bottom of bottle channel in extraction flask, so that the logistics to be extracted in it is gone out to realize to rack sample to be tested and carry out situ extracting operation, sample pollution and loss are avoided, it is easy to use.
Description
Technical field
The present invention relates to a kind of solid phase microextraction pre-treatment component and solid-phase micro-extracting devices.
Background technology
Solid phase microextraction be using the vitreous silica fiber for being coated with stationary phase adsorb, the test substance in enriched sample,
Analyte is concentrated while extraction, the fiber for being enriched determinand is then transferred directly to liquid phase or gas-chromatography
In, carry out sample analysis.There are a distinct issues in existing solid-phase micro-extracting device, i.e. extraction container is limited when in use
It is set to fixed absorption bottle, this many times to also need to be transferred to from experiment container by the sample for detaching, being concentrated to give
Sorption extraction could be carried out in fixed absorption bottle, and sample is inevitably caused to pollute and lose in transfer process, especially
It is generally difficult to be shifted for some experiment gained trace samplings.
In view of the above-mentioned problems, disclosing one kind in the Chinese utility model patent that Authorization Notice No. is 206593939 U of CN
Suitable for the device of solid phase microextraction in-site detecting, including extraction needle, adapter and ground glass stoppered bottle, adapter is hollow knot
Structure, lower, outer perimeter wall are the ground structure coordinated with ground glass stoppered bottle, and upper periphery wall is threaded, and are embedded with gasket
Sealing cover screw-thread fit, sealing hd top face in the middle part of offer open-work.In use, extraction needle is inserted into ground glass across sealing cover
In glass bottle, situ extracting is carried out to the sample in ground glass stoppered bottle.
The device suitable for solid phase microextraction in-site detecting involved by above-mentioned patent is in liquid phase or gas phase object to be extracted
Situ extracting is carried out when being placed into ground glass stoppered bottle, and object to be extracted is wrapped in the sample with shell
Situation but and is not suitable for, and such case must first rack sample before being extracted makes the gas phase in it or liquid phase object to be extracted
Situ extracting operation could be carried out by flowing out in container.
Invention content
The purpose of the present invention is to provide a kind of can rack sample to be tested so that the logistics to be extracted in it is gone out and carry out
The solid phase microextraction pre-treatment component of situ extracting;The present invention also aims to provide a kind of solid-phase micro-extracting device.
To achieve the above object, solid phase microextraction pre-treatment component of the invention includes extraction flask, and extraction flask bottle mouth position is set
Have for extraction needle hermetically passing and be inserted into the sealing structure in extraction flask, which further includes square in an axial direction with extraction flask
The inner cavity of the squeeze bottle coordinated to air-tight movable, squeeze bottle constitutes the accommodation space for placing sample and in squeeze bottle close to extraction
It can coordinate with the bottom of bottle of extraction flask when bottle being taken to move and rack sample, the bottom of bottle of extraction flask is additionally provided in the sample for Gong being squeezed and broken
Object to be extracted enter the channel of extraction flask.Sample is placed in squeeze bottle, squeeze bottle is made to be moved close to extraction flask until sample
Product are squeezed and broken, and the object to be extracted in sample is entered by extraction flask bottom of bottle channel in extraction flask, to realize sample to be tested
Racking makes the logistics to be extracted in it go out and carries out situ extracting operation, avoids sample pollution and loss, easy to use.
Further, the periphery wall of the extraction flask is equipped with external screw thread, and the internal perisporium of the squeeze bottle is equipped with interior spiral shell
Line, extraction flask realize air-tight movable with squeeze bottle by screw-thread fit.
Sealing structure set by extraction flask bottle mouth position is the rubber seal blocked at extraction flask position of bottleneck.
Further, the rubber seal uses rubber gasket.
When being sealed using rubber gasket, it need to ensure that gasket is pressed in extraction flask bottle mouth position, therefore, extract
A bottle bottle mouth position setting clamping cap is taken, for compressing rubber gasket.
The mode that clamping cap compresses gasket has very much, specifically, clamping cap may be used and match with extraction flask whorl of bottleneck
The mode of conjunction, and the extraction needle perforation passed through for extraction needle is opened up on clamping cap.
The solid-phase micro-extracting device of the present invention includes extraction needle and extraction flask, and extraction flask bottle mouth position is equipped with close for extraction needle
It seals across and is inserted into the sealing structure in extraction flask, which further includes crowded with extraction flask in axial direction air-tight movable cooperation
The inner cavity of pressure bottle, squeeze bottle constitutes the accommodation space for placing sample and can be with extraction when squeeze bottle is moved close to extraction flask
It takes the bottom of bottle of bottle to coordinate to rack sample, the bottom of bottle of extraction flask is additionally provided with the object to be extracted in the sample for Gong being squeezed and broken and enters extraction
The channel of bottle.Sample is placed in squeeze bottle, so that squeeze bottle is moved close to extraction flask until sample is squeezed and broken, is waited in sample
Extract is entered by extraction flask bottom of bottle channel in extraction flask, to realize the object to be extracted for racking sample to be tested and making in it
Situ extracting operation is flowed out and carried out, sample pollution and loss are avoided, it is easy to use.
Further, the periphery wall of the extraction flask is equipped with external screw thread, and the internal perisporium of the squeeze bottle is equipped with interior spiral shell
Line, extraction flask realize air-tight movable with squeeze bottle by screw-thread fit.
Sealing structure set by extraction flask bottle mouth position is the rubber seal blocked at extraction flask position of bottleneck.
Further, the rubber seal uses rubber gasket.
When being sealed using rubber gasket, it need to ensure that gasket is pressed in extraction flask bottle mouth position, therefore, extract
A bottle bottle mouth position setting clamping cap is taken, for compressing rubber gasket.
The mode that clamping cap compresses gasket has very much, specifically, clamping cap may be used and match with extraction flask whorl of bottleneck
The mode of conjunction, and the extraction needle perforation passed through for extraction needle is opened up on clamping cap.
Description of the drawings
Fig. 1 is the structural schematic diagram of the embodiment 10 of the solid-phase micro-extracting device of the present invention;
Fig. 2 is the structural schematic diagram of extraction flask bottom of bottle in Fig. 1;
In figure:1, needle is extracted;2, clamping cap;3, rubber gasket;4, extraction flask;5, channel;6, squeeze bottle.
Specific implementation mode
Embodiments of the present invention are described further below in conjunction with the accompanying drawings.
The present invention provides the numerous embodiments of solid-phase micro-extracting device.Such as embodiment 1, solid-phase micro-extracting device includes
Needle and solid phase microextraction pre-treatment component are extracted, which includes extraction flask, and extraction flask bottle mouth position is equipped with to extract needle
Hermetically passing and be inserted into the sealing structure in extraction flask, which further includes that in axial direction air-tight movable is matched with extraction flask
The inner cavity of the squeeze bottle of conjunction, squeeze bottle constitutes the accommodation space for placing sample and the energy when squeeze bottle is moved close to extraction flask
Enough bottom of bottle cooperations with extraction flask rack sample, the bottom of bottle of extraction flask be additionally provided with the object to be extracted in the sample for Gong being squeezed and broken into
Enter the channel of extraction flask.
Squeeze bottle and the mode of extraction flask in axial direction air-tight movable cooperation can be screw-thread fit or sealed extruded etc.
Mode, in embodiment 2, it is preferable that use thread fiting mode, that is, external screw thread is equipped on extraction flask periphery wall, in squeeze bottle
Internal perisporium is equipped with internal thread, and extraction flask realizes air-tight movable with squeeze bottle by screw-thread fit.Certainly, in other embodiment
In, squeeze bottle can also be kept gradually close to extraction flask, racked until by sample by the way of sealed extruded.
Embodiment 3:On the basis of embodiment 1 or 2, sealing structure is the rubber seal blocked in extraction flask bottle mouth position
Part.
It is rubber gasket that embodiment 4, which further defines the rubber seal in embodiment 3, in other embodiments,
Can also be rubber seal plug or rubber plug.
It when in view of being sealed using rubber gasket, need to ensure that gasket is pressed in extraction flask bottle mouth position, implementing
Clamping cap further is set in extraction flask bottle mouth position in example 5, for compressing rubber gasket.
The mode that clamping cap compresses gasket has very much, in embodiment 6, specifically, using clamping cap and extraction flask bottle
The mode of mouth screw-thread fit, and the extraction needle perforation passed through for extraction needle is opened up on clamping cap.In other embodiments,
Turnup structure can be set respectively in the upper and lower part of clamping cap, wherein upper overturning compresses rubber gasket, and lower flange is pressed on extraction
Bottle bottle mouth position, to realize the purpose for compressing gasket.
Solid-phase micro-extracting device is in practical application, need to design extraction flask and squeeze bottle according to the size of sample to be tested
Size.For example, embodiment 7 just gives the parameter setting of extraction flask and squeeze bottle when sample to be tested pearl quick-fried for cigarette, tool
Body, the internal diameter of the bottleneck of extraction flask is 20-35mm, and the body outer diameter of extraction flask is 25-40mm, and the bottle height of extraction flask is 40-
The outer diameter of 80mm, squeeze bottle are 27-42mm, and the height of squeeze bottle is 45-85mm.
In embodiment 8, the internal diameter of the bottleneck of extraction flask is 20mm, and the body outer diameter of extraction flask is 25mm, the bottle of extraction flask
A height of 40mm, corresponding, the outer diameter of squeeze bottle is 27mm, and the height of squeeze bottle is 45mm.
In embodiment 9, the internal diameter of the bottleneck of extraction flask is 35mm, and the body outer diameter of extraction flask is 40mm, the bottle of extraction flask
High 80mm, accordingly, the outer diameter of squeeze bottle is 42mm, and the height of squeeze bottle is 85mm.
The present invention also provides the specific embodiments 10 of solid-phase micro-extracting device, broken and former for being carried out to the quick-fried pearl of cigarette
Position extraction.As shown in Figure 1 to Figure 2, solid-phase micro-extracting device include extraction needle 1, extraction flask 4 and with extraction flask 4 in an axial direction side
To the squeeze bottle 6 coordinated by thread seal activity, the inner cavity of squeeze bottle 6 constitutes the accommodation space for placing quick-fried pearl and is squeezing
Pressure bottle 6 can coordinate when being moved close to extraction flask 4 with the bottom of bottle of extraction flask 4 racks quick-fried pearl, and the bottom of bottle of extraction flask 4 is equipped with for quilt
The object to be extracted in quick-fried pearl racked enters the channel 5 of extraction flask 4, and channel size is less than quick-fried pearl diameter.The bottle mouth position of extraction flask 4
Clamping cap 2 equipped with rubber gasket 3 and for compressing rubber gasket 3, clamping cap 2 coordinate with extraction flask whorl of bottleneck,
The extraction needle perforation passed through for extraction needle is offered on clamping cap 2, it is right in extraction flask that extraction needle 1 passes through rubber gasket to be inserted into
Object to be extracted is adsorbed, is enriched with.The internal diameter of the bottleneck of extraction flask 4 is 20mm, and the body outer diameter of extraction flask 4 is 25mm, extraction flask 4
Bottle height be 40mm, corresponding, the outer diameter of squeeze bottle 6 is 27mm, and the bottle height of squeeze bottle 6 is 45mm.
When being measured to the quick-fried pearl of cigarette, quick-fried pearl is placed in squeeze bottle first, by rotating extraction flask and/or squeeze bottle
Both make constantly close, extraction flask bottom of bottle moves closer to quick-fried pearl position, extraction flask cavity internal pressure be gradually increased until by
Quick-fried pearl racks, in logistics to be extracted go out and entered in extraction flask by extraction flask bottom of bottle channel, will be to be measured to realize
Sample, which racks, to be made the logistics to be extracted in it go out and carries out situ extracting operation, and sample pollution and loss are avoided, easy to use.
The present invention also provides a kind of embodiments of solid phase microextraction pre-treatment component, and concrete structure is in above-mentioned solid phase
It has been discussed in detail in the embodiment of micro-extraction device, details are not described herein again.
Claims (12)
1. a kind of solid phase microextraction pre-treatment component, characterized in that including extraction flask, extraction flask bottle mouth position is equipped with to extract needle
Hermetically passing and be inserted into the sealing structure in extraction flask, which further includes that in axial direction air-tight movable is matched with extraction flask
The inner cavity of the squeeze bottle of conjunction, squeeze bottle constitutes the accommodation space for placing sample and the energy when squeeze bottle is moved close to extraction flask
Enough bottom of bottle cooperations with extraction flask rack sample, the bottom of bottle of extraction flask be additionally provided with the object to be extracted in the sample for Gong being squeezed and broken into
Enter the channel of extraction flask.
2. solid phase microextraction pre-treatment component according to claim 1, characterized in that set on the periphery wall of the extraction flask
There are external screw thread, the internal perisporium of the squeeze bottle to be equipped with internal thread, extraction flask realizes that sealing is lived with squeeze bottle by screw-thread fit
It is dynamic.
3. solid phase microextraction pre-treatment component according to claim 1 or 2, characterized in that the sealing structure is to block
Rubber seal at extraction flask position of bottleneck.
4. solid phase microextraction pre-treatment component according to claim 3, characterized in that the rubber seal is that rubber is close
Packing.
5. solid phase microextraction pre-treatment component according to claim 4, characterized in that extraction flask bottle mouth position, which is additionally provided with, to be used for
Compress the clamping cap of the rubber gasket.
6. solid phase microextraction pre-treatment component according to claim 5, characterized in that the clamping cap and extraction flask bottleneck
Screw-thread fit and offered on clamping cap for extraction needle pass through extraction needle perforation.
7. solid-phase micro-extracting device, characterized in that including extraction needle and extraction flask, extraction flask bottle mouth position is equipped with close for extraction needle
It seals across and is inserted into the sealing structure in extraction flask, which further includes crowded with extraction flask in axial direction air-tight movable cooperation
The inner cavity of pressure bottle, squeeze bottle constitutes the accommodation space for placing sample and can be with extraction when squeeze bottle is moved close to extraction flask
It takes the bottom of bottle of bottle to coordinate to rack sample, the bottom of bottle of extraction flask is additionally provided with the object to be extracted in the sample for Gong being squeezed and broken and enters extraction
The channel of bottle.
8. solid-phase micro-extracting device according to claim 7, characterized in that the periphery wall of the extraction flask is equipped with outer spiral shell
The internal perisporium of line, the squeeze bottle is equipped with internal thread, and extraction flask realizes air-tight movable with squeeze bottle by screw-thread fit.
9. solid-phase micro-extracting device according to claim 7 or 8, characterized in that the sealing structure is to block extracting
Rubber seal at bottle position of bottleneck.
10. solid-phase micro-extracting device according to claim 9, characterized in that the rubber seal is rubber gasket.
11. solid-phase micro-extracting device according to claim 10, characterized in that extraction flask bottle mouth position is additionally provided with for compressing
The clamping cap of the rubber gasket.
12. solid-phase micro-extracting device according to claim 11, characterized in that the clamping cap and extraction flask whorl of bottleneck
Coordinate and offers the extraction needle perforation passed through for extraction needle on clamping cap.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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US11078442B2 (en) * | 2017-02-03 | 2021-08-03 | Symrise Ag | Method and test kit for recreation of an odor |
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