CN108310054A - A kind of dandelion compound extract extracted using supercritical carbon dioxide - Google Patents
A kind of dandelion compound extract extracted using supercritical carbon dioxide Download PDFInfo
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- CN108310054A CN108310054A CN201810347282.0A CN201810347282A CN108310054A CN 108310054 A CN108310054 A CN 108310054A CN 201810347282 A CN201810347282 A CN 201810347282A CN 108310054 A CN108310054 A CN 108310054A
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- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 title claims abstract description 59
- 241000245665 Taraxacum Species 0.000 title claims abstract description 57
- 150000001875 compounds Chemical class 0.000 title claims abstract description 25
- 239000000284 extract Substances 0.000 title claims abstract description 25
- 229910002092 carbon dioxide Inorganic materials 0.000 title abstract description 21
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 title abstract description 8
- 239000001569 carbon dioxide Substances 0.000 title abstract description 4
- 238000000605 extraction Methods 0.000 claims abstract description 53
- 244000113428 Sonchus oleraceus Species 0.000 claims abstract description 29
- 235000006745 Sonchus oleraceus Nutrition 0.000 claims abstract description 29
- 235000004347 Perilla Nutrition 0.000 claims abstract description 28
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000000287 crude extract Substances 0.000 claims abstract description 18
- 229910021538 borax Inorganic materials 0.000 claims abstract description 16
- 239000004328 sodium tetraborate Substances 0.000 claims abstract description 16
- 235000010339 sodium tetraborate Nutrition 0.000 claims abstract description 16
- 238000010992 reflux Methods 0.000 claims abstract description 10
- 239000000706 filtrate Substances 0.000 claims abstract description 9
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 244000124853 Perilla frutescens Species 0.000 claims abstract 4
- 238000000926 separation method Methods 0.000 claims description 18
- 239000000047 product Substances 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 15
- 230000003712 anti-aging effect Effects 0.000 claims description 9
- 230000000694 effects Effects 0.000 claims description 9
- 230000036541 health Effects 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 239000004615 ingredient Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 description 27
- 241000229722 Perilla <angiosperm> Species 0.000 description 24
- 230000032683 aging Effects 0.000 description 9
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 229930003427 Vitamin E Natural products 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 229930182830 galactose Natural products 0.000 description 3
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 3
- 230000002496 gastric effect Effects 0.000 description 3
- 238000001802 infusion Methods 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 235000019165 vitamin E Nutrition 0.000 description 3
- 229940046009 vitamin E Drugs 0.000 description 3
- 239000011709 vitamin E Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 240000001949 Taraxacum officinale Species 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 150000003712 vitamin E derivatives Chemical group 0.000 description 2
- 206010007247 Carbuncle Diseases 0.000 description 1
- 235000008495 Chrysanthemum leucanthemum Nutrition 0.000 description 1
- 244000192528 Chrysanthemum parthenium Species 0.000 description 1
- 235000000604 Chrysanthemum parthenium Nutrition 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 230000035508 accumulation Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- GZCGUPFRVQAUEE-KCDKBNATSA-N aldehydo-D-galactose Chemical compound OC[C@@H](O)[C@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-KCDKBNATSA-N 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000008384 feverfew Nutrition 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 230000004768 organ dysfunction Effects 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 230000037081 physical activity Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 230000003637 steroidlike Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003815 supercritical carbon dioxide extraction Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/535—Perilla (beefsteak plant)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Medical Informatics (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
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- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to a kind of dandelion compound extract extracted using supercritical carbon dioxide, the preparation method of the dandelion compound extract includes the following steps：(1) after crushing dandelion, common sowthistle root, perilla leaf, suitable borax solution is added, after impregnating 12 hours, the ethanol solution refluxing extraction that volume fraction is 20 30% is added after 56 hours, it filters, after filtrate tune pH to 5.0 6.0, is concentrated to give crude extract；(2) crude extract for obtaining step (1) is added in extraction kettle, using supercritical CO2Extraction, obtains the dandelion compound extract.
Description
Technical field
The invention belongs to natural plant health care product fields, and in particular to a kind of Pu using supercritical carbon dioxide extraction is public
English compound extract.
Background technology
Dandelion is the drying herb of composite family Dandelion herbaceos perennial, bitter, sweet, cold in nature, and tool is clearing heat and detoxicating,
The effect of dispersing swelling and dissipating binds, inducing diuresis for treating strangurtia.First recorded in《Tang materia medica》：" leaf spends Huang like hare's-lettuce, disconnected to have white sauce, people all to eat it.Housewife
Human milk carbuncle swells.”.Common sowthistle root is the root of feverfew hare's-lettuce, has the effect of hypoglycemic, lipid-loweringing, clearing deficient heat.Perilla leaf is for I
State's tradition heat-clearing and detoxifying herb, containing volatile oil, flavones and anthocyanin class, phenolic acid class, glycoside, triterpenes and steroidal in perilla leaf
Etc. a variety of chemical compositions, perilla leaf extract shows certain anti-inflammatory activity.
Aging is the comprehensive complicated physiology of body molecule, cell, organ dysfunction or even entire deterioration, pathological change
Process is the abbreviation of senescence phase variation.Oxidative stress theory thinks aging early stage, and the active oxygen of low dosage being capable of exactor
The protective pressure stress reaction of body slows down aging；Increasing when the age, the relevant oxidative damage of aging is persistently assembled in vivo,
It has been more than the Scavenging activity of body, the active oxygen of these accumulations aggravates aging-related DNA damage, accelerates the aging of cell.Cause
This, which develops a kind of natural exogenous material being able to maintain that body activity oxygen balance, becomes the research hotspot in anti-aging field.
Invention content
The present invention provides a kind of dandelion Halth-care composition, it is characterised in that by weight, the composition it is effective
Ingredient includes 3-5 parts of dandelion, 1-2 parts of common sowthistle root, 0.1-0.3 parts of perilla leaf.
Another embodiment of the present invention provides a kind of dandelion Halth-care composition, it is characterised in that by weight,
The composition preferably comprises 3 parts of dandelion, 1 part of common sowthistle root, 0.2 part of perilla leaf.
Another embodiment of the present invention provides a kind of dandelion compound extract, it is characterised in that the dandelion compound
The preparation method of extract includes the following steps：
(1) according to above-mentioned dandelion Halth-care composition described in parts by weight, by dandelion, common sowthistle root, perilla leaf powder
After broken, suitable borax solution is added, after impregnating 1-2 hours, the ethanol solution reflux that volume fraction is 20-30% is added and carries
It after taking 5-6 hours, filters, after filtrate tune pH to 5.0-6.0, is concentrated to give crude extract；
(2) crude extract for obtaining step (1) is added in extraction kettle, using supercritical CO2It is multiple to obtain the dandelion for extraction
Formula extraction.
Step (1) crushing is preferably crushed to 20-40 mesh, and the dosage of borax solution is dandelion, common sowthistle root, purple perilla
The mass concentration of 2.0-2.5 times of the sum of leaf quality, borax solution is 2-4%；Extracting pressure is 26- in step (2)
28MPa, extraction temperature are 45-48 DEG C, CO2Flow is 50-55L/h, and extraction time 2-3h, separation reactor I temperature is 40-42
DEG C, pressure 8-10MPa, separation reactor I I temperature is 35-38 DEG C, pressure 5-6MPa.
Another embodiment of the present invention provides a kind of preparation method of dandelion compound extract, it is characterised in that including
Following steps：
(1) according to above-mentioned dandelion Halth-care composition described in parts by weight, by dandelion, common sowthistle root, perilla leaf powder
After broken, suitable borax solution is added, after impregnating 1-2 hours, the ethanol solution reflux that volume fraction is 20-30% is added and carries
It after taking 5-6 hours, filters, after filtrate tune pH to 5.0-6.0, is concentrated to give crude extract；
(2) crude extract for obtaining step (1) is added in extraction kettle, using supercritical CO2It is multiple to obtain the dandelion for extraction
Formula extraction.
Step (1) crushing is preferably crushed to 20-40 mesh, and the dosage of borax solution is dandelion, common sowthistle root, purple perilla
The mass concentration of 2.0-2.5 times of the sum of leaf quality, borax solution is 2-4%；Extracting pressure is 26- in step (2)
28MPa, extraction temperature are 45-48 DEG C, CO2Flow is 50-55L/h, and extraction time 2-3h, separation reactor I temperature is 40-42
DEG C, pressure 8-10MPa, separation reactor I I temperature is 35-38 DEG C, pressure 5-6MPa.
Another embodiment of the present invention provides a kind of composition, it is characterised in that the composition is multiple comprising above-mentioned dandelion
Formula extraction.The composition may also include other activity of fighting against senium ingredients.The activity of fighting against senium ingredient includes antiaging agent
Or antisenescence health product.
Another embodiment of the present invention provides above-mentioned dandelion compound extract and is preparing antiaging agent or anti-aging
Application in health products.
Another embodiment of the present invention provide above-mentioned dandelion compound extract in improving serum and/or tissue SOD,
Application in CAT, GSH-Px, T-Aoc enzyme activity.
Another embodiment of the present invention provides above-mentioned dandelion compound extract MDA in reducing serum and/or tissue
Application in content.
The parts by weight of dandelion of the present invention, common sowthistle root, perilla leaf are dry weight.
Compared with the prior art, the advantages of the present invention are as follows：(1) present invention is impregnated by borax solution, ethanol solution
Extraction uses the method for supercritical carbon dioxide extracting to obtain dandelion, common sowthistle root, perilla leaf compound extract after adjusting pH, should
Extract can significantly reduce the content of MDA in D- galactolipin aging mices, improve SOD in mouse aging serum and brain tissue,
CAT, GSH-Px, T-Aoc enzyme activity show that the extract is expected to be exploited for antiaging agent or antisenescence health product.(2)
In the case where inventor makes the creative labor, obtained dandelion in compound extract, common sowthistle root, perilla leaf formula,
Wherein perilla leaf plays the role of a kind of accompanying plant, i.e., when association medicine perilla leaf dosage is in a certain range, can promote main ingredient
Certain active constituents dissolve out, are easy to be absorbed in dandelion and common sowthistle root, play pharmacological activity.
Specific implementation mode
For the ease of a further understanding of the present invention, examples provided below has done more detailed description to it.But
It is that these embodiments are only not supposed to be a limitation to the present invention or implementation principle for being better understood from invention, reality of the invention
The mode of applying is not limited to the following contents.
Embodiment 1
Formula：3 parts of dandelion, 1 part of common sowthistle root, 0.2 part of perilla leaf.
(1) according to formula ratio, after dandelion (300g), common sowthistle root (100g), perilla leaf (20g) are crushed to 20-40 mesh,
2% borax solution (840g) is added, after impregnating 2 hours, the ethanol solution refluxing extraction that addition volume fraction is 20% 6 hours
Afterwards, it filters, after filtrate tune pH to 5.0-6.0, is concentrated to give crude extract；
(2) crude extract for obtaining step (1) is added in extraction kettle, using supercritical CO2It is multiple to obtain the dandelion for extraction
Formula extraction 18.9g (hereinafter referred to as product A)；Wherein, extracting pressure 26MPa, extraction temperature are 45-46 DEG C, CO2Flow is
50L/h, extraction time 3h, separation reactor I temperature are 40-42 DEG C, pressure 8MPa, and separation reactor I I temperature is 35-38 DEG C, pressure
For 5MPa.
Embodiment 2
Formula：5 parts of dandelion, 1 part of common sowthistle root, 0.1 part of perilla leaf.
(1) according to formula ratio, after dandelion (500g), common sowthistle root (100g), perilla leaf (10g) are crushed to 20-40 mesh,
4% borax solution (1525g) is added, after impregnating 1 hour, it is small that the ethanol solution refluxing extraction 5 that volume fraction is 30% is added
Shi Hou is filtered, and after filtrate tune pH to 5.0-6.0, is concentrated to give crude extract；
(2) crude extract for obtaining step (1) is added in extraction kettle, using supercritical CO2It is multiple to obtain the dandelion for extraction
Formula extraction 30.6g (hereinafter referred to as product B)；Wherein, extracting pressure 28MPa, extraction temperature are 46-48 DEG C, CO2Flow is
55L/h, extraction time 2h, separation reactor I temperature are 40-42 DEG C, pressure 10MPa, and separation reactor I I temperature is 35-38 DEG C, pressure
Power is 6MPa.
Embodiment 3
Formula：3 parts of dandelion, 2 parts of common sowthistle root, 0.3 part of perilla leaf.
(1) according to formula ratio, after dandelion (300g), common sowthistle root (200g), perilla leaf (30g) are crushed to 20-40 mesh,
3% borax solution (1100g) is added, after impregnating 1.5 hours, the ethanol solution refluxing extraction 5 that volume fraction is 25% is added
It after hour, filters, after filtrate tune pH to 5.0-6.0, is concentrated to give crude extract；
(2) crude extract for obtaining step (1) is added in extraction kettle, using supercritical CO2It is multiple to obtain the dandelion for extraction
Formula extraction 20.2g (hereinafter referred to as products C)；Wherein, extracting pressure 26MPa, extraction temperature are 45-46 DEG C, CO2Flow is
50L/h, extraction time 3h, separation reactor I temperature are 40-42 DEG C, pressure 8MPa, and separation reactor I I temperature is 35-38 DEG C, pressure
For 5MPa.
Embodiment 4
Formula：3 parts of dandelion, 1 part of common sowthistle root, 0.02 part of perilla leaf.
(1) according to formula ratio, after dandelion (300g), common sowthistle root (100g), perilla leaf (2g) are crushed to 20-40 mesh,
2% borax solution (840g) is added, after impregnating 2 hours, the ethanol solution refluxing extraction that addition volume fraction is 20% 6 hours
Afterwards, it filters, after filtrate tune pH to 5.0-6.0, is concentrated to give crude extract；
(2) crude extract for obtaining step (1) is added in extraction kettle, using supercritical CO2Extraction, obtains dandelion compound and carries
Take object 10.6g (hereinafter referred to as product D)；Wherein, extracting pressure 26MPa, extraction temperature are 45-46 DEG C, CO2Flow is 50L/
H, extraction time 3h, separation reactor I temperature are 40-42 DEG C, pressure 8MPa, and separation reactor I I temperature is 35-38 DEG C, and pressure is
5MPa。
Embodiment 5
Formula：3 parts of dandelion, 1 part of common sowthistle root.
(1) according to formula ratio, after dandelion (300g), common sowthistle root (100g) are crushed to 20-40 mesh, 2% borax is added
Aqueous solution (840g) after impregnating 2 hours, is added the ethanol solution refluxing extraction that volume fraction is 20% after 6 hours, filters, filter
After liquid tune pH to 5.0-6.0, it is concentrated to give crude extract；
(2) crude extract for obtaining step (1) is added in extraction kettle, using supercritical CO2Extraction, obtains dandelion compound and carries
Take object 6.5g (hereinafter referred to as product E)；Wherein, extracting pressure 26MPa, extraction temperature are 45-46 DEG C, CO2Flow is 50L/
H, extraction time 3h, separation reactor I temperature are 40-42 DEG C, pressure 8MPa, and separation reactor I I temperature is 35-38 DEG C, and pressure is
5MPa。
Embodiment 6
Formula：3 parts of dandelion, 1 part of common sowthistle root, 0.2 part of perilla leaf.
(1) according to formula ratio, after dandelion (300g), common sowthistle root (100g), perilla leaf (20g) are crushed to 20-40 mesh,
The ethanol solution refluxing extraction that volume fraction is 20% is added after 6 hours, filters, after filtrate tune pH to 5.0-6.0, is concentrated to give thick
Extract；
(2) crude extract for obtaining step (1) is added in extraction kettle, using supercritical CO2Extraction, obtains dandelion compound and carries
Take object 9.2g (hereinafter referred to as product F)；Wherein, extracting pressure 26MPa, extraction temperature are 45-46 DEG C, CO2Flow is 50L/
H, extraction time 3h, separation reactor I temperature are 40-42 DEG C, pressure 8MPa, and separation reactor I I temperature is 35-38 DEG C, and pressure is
5MPa。
Embodiment 7
The anti-aging effects of product A-F of the present invention carry out pharmacological experiment using D- galactolipin Aging mice models.
D- galactose solutions configure：D- galactolipins are dissolved in physiological saline, are made into a concentration of 20mg/mL.(intraperitoneal injection)
Vitamin E (VE) solution allocation：It is configured to 20mg/mL with vegetable oil.(gastric infusion)
Model group：D- galactose solutions, dosage 100mg/kg/d is injected intraperitoneally；
Normal group：The physiological saline of equivalent is injected intraperitoneally；
VE groups：D- galactose solutions, dosage 100mg/kg/d, while gastric infusion VE solution, dosage is injected intraperitoneally
100mg/kg/d；
Product A-F groups：D- galactose solutions, dosage 100mg/kg/d is injected intraperitoneally, while gastric infusion is corresponding respectively
Product A-F solution, dosage 200mg/kg/d.
The present invention tests SOD, CAT, GSH-Px, T-Aoc enzyme activity and MDA contents in mice serum, brain, specific experiment side
Method uses Chinese invention patent application number：The method that embodiment 2 is recorded in CN201710674001.8 carries out.As a result it see the table below.
Experimental data, compared with normal group, model group has statistical significance (p<0.05), compared with model group, VE groups and product A-F
Group also has statistical significance (p<0.05).
Claims (8)
1. a kind of dandelion compound extract, it is characterised in that the preparation method of the dandelion compound extract includes following step
Suddenly：
(1) after crushing dandelion, common sowthistle root, perilla leaf, suitable borax solution is added, after impregnating 1-2 hours, body is added
Fraction is the ethanol solution refluxing extraction after 5-6 hour of 20-30%, filtering, after filtrate tune pH to 5.0-6.0, is concentrated to give slightly
Extract；
(2) crude extract for obtaining step (1) is added in extraction kettle, using supercritical CO2Extraction, obtains the dandelion compound and carries
Take object；
Wherein by weight, step (1) uses 3-5 parts of dandelion, 1-2 parts of common sowthistle root, 0.1-0.3 parts of perilla leaf.
2. dandelion compound extract described in claim 1, it is characterised in that step (1) crushing is preferably crushed to 20-
40 mesh.
3. claim 1-2 any one of them dandelion compound extracts, it is characterised in that the dosage of borax solution is Pu
The mass concentration of 2.0-2.5 times of public English, common sowthistle root, perilla leaf quality sum, borax solution is 2-4%.
4. claim 1-3 any one of them dandelion compound extracts, it is characterised in that extracting pressure is in step (2)
26-28MPa, extraction temperature are 45-48 DEG C, CO2Flow is 50-55L/h, and extraction time 2-3h, separation reactor I temperature is 40-
42 DEG C, pressure 8-10MPa, separation reactor I I temperature is 35-38 DEG C, pressure 5-6MPa.
5. a kind of composition, it is characterised in that the composition is extracted comprising claim 1-5 any one of them dandelion compounds
Object.
6. the composition described in claim 5, it is characterised in that the composition may also include other activity of fighting against senium ingredients.
7. the composition described in claim 6, it is characterised in that the activity of fighting against senium ingredient includes antiaging agent or anti-ageing
Old health products.
8. claim 1-5 any one of them dandelion compound extracts are in preparing antiaging agent or antisenescence health product
Application.
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| CN201810347282.0A CN108310054A (en) | 2018-04-17 | 2018-04-17 | A kind of dandelion compound extract extracted using supercritical carbon dioxide |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109121778A (en) * | 2018-08-07 | 2019-01-04 | 广西田东县东辰农业开发有限公司 | It is a kind of slowly take root tumor fungus extract extracting method and its mango grafting on application |
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| CN107114523A (en) * | 2016-02-24 | 2017-09-01 | 广西贵港市港南区鸿德源铁皮石斛种植农民专业合作社 | A kind of processing method of purple perilla powder |
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Application publication date: 20180724 |