CN108308181B - Plant vegetable growth regulator and application - Google Patents
Plant vegetable growth regulator and application Download PDFInfo
- Publication number
- CN108308181B CN108308181B CN201810175156.1A CN201810175156A CN108308181B CN 108308181 B CN108308181 B CN 108308181B CN 201810175156 A CN201810175156 A CN 201810175156A CN 108308181 B CN108308181 B CN 108308181B
- Authority
- CN
- China
- Prior art keywords
- plant
- growth regulator
- gly
- vegetable
- vegetable growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a plant vegetable growth regulator, belonging to the technical field of vegetable planting and cultivation, and comprising the following components in parts by weight: HrpNEccPR0.1-0.5 part of protein and 90-95 parts of water; the HrpNEccPRThe amino acid sequence of the protein is shown as SEQ ID No. 1. Compared with water and plant growth regulators, the plant vegetable growth regulator provided by the invention improves the yield and quality of leaf lettuce, cucumber, Chinese cabbage, tomato and cabbage mustard, and has no chemical harmful substance residue, no public nuisance and no pollution.
Description
Technical Field
The invention belongs to the technical field of vegetable planting and cultivation, and particularly relates to a plant vegetable growth regulator and application thereof.
Background
The vegetables are one of the essential foods in daily diet of people, can provide various nutrients such as vitamins and minerals necessary for human bodies, can effectively prevent chronic and degenerative diseases, and according to the statistics of the international food and rindergard organization in 1990, 90 percent of VC and 60 percent of VA necessary for human bodies come from the vegetables. Therefore, the demand of the public for vegetable dishes is increasing.
At present, substances applied to the vegetable yield increase aspect are mainly chemical preparations and hormone products, such as triacontanol, fulvic acid, urea and the like, and the yield increase effect of the products is between 10 and 30 percent. The existing method has chemical residue after use, causes pollution of different degrees to products and environment, has adverse effect on the quality of the products, and does not meet the requirement of green food production.
Disclosure of Invention
The invention aims to provide a plant vegetable growth regulator which can improve the yield of plant vegetables, is nontoxic, pollution-free and residue-free and meets the requirements of green food.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a plant vegetable growth regulator which comprises the following components in parts by weight:
HrpNEccPR0.1-0.5 part of protein and 90-95 parts of water; the HrpNEccPRThe amino acid sequence of the protein is shown as SEQ ID No. 1.
Preferably, the method further comprises the following steps: 5-8 parts of proteolytic amino acid.
The invention also provides application of the plant vegetable growth regulator in the technical scheme in improving the yield and/or quality of plant vegetables.
Preferably, the vegetable includes leaf lettuce, cucumber, Chinese cabbage, tomato, cabbage mustard, chili, pumpkin, carrot or soybean.
Preferably, the application is: and mixing the plant vegetable growth regulator with seeds of plant vegetables for seed soaking, wherein the mass ratio of the plant vegetable growth regulator to the seeds of the plant vegetables is 1: 1000-3500.
Preferably, the application is: and (3) diluting the plant vegetable growth regulator by 500-1500 times, and spraying the plant vegetable growth regulator to plant vegetables, wherein the amount of the plant vegetable growth regulator sprayed each time is 2-30 g/mu.
The invention provides a plant vegetable growth regulator which comprises the following components in parts by weight: HrpNEccPR0.1-0.5 part of protein and 90-95 parts of water; the HrpNEccPRThe amino acid sequence of the protein is shown as SEQ ID No. 1. HrpN in plant vegetable growth regulator provided by the inventionEccPRThe protein can increase the absorption of the soil nutrient components by the vegetables, has good promotion effect on the growth of the plant vegetables, and further can improve the yield of the plant vegetables.
The results of the embodiments of the present invention show that: compared with water and plant growth regulators, the plant vegetable growth regulator provided by the invention improves the yield and quality of leaf lettuce, cucumber, Chinese cabbage, tomato and cabbage mustard, and has no chemical harmful substance residue, no public nuisance and no pollution.
Detailed Description
The invention provides a plant vegetable growth regulator which comprises the following components in parts by weight: HrpNEccPR0.1-0.5 part of protein and 90-95 parts of water; the HrpNEccPRThe amino acid sequence of the protein is shown as SEQ ID No. 1.
The plant vegetable growth regulator provided by the invention comprises 0.1-0.5 parts by weight of HrpNEccPRThe protein is preferably 0.2-0.4 parts. In the present invention, the HrpN isEccPRThe protein can enhance the resistance of the plant vegetables, can increase the absorption of the plant vegetables to soil nutrient components, has good promotion effect on the growth of the plant vegetables, and further can improve the yield of the plant vegetables. By HrpNEccPRThe protein is tested in different crops or vegetables, and the protein has different functions under different concentrations, when the concentration of the protein exceeds 1%, the protein mainly plays a role in preventing and treating diseases, and when the concentration of the protein is less than 0.1%, the protein has a certain growth promoting effect on the plants.
In the present invention, the HrpN isEccPRThe amino acid sequence of the protein is shown as SEQ ID No.1, and is specifically shown as follows:
Met LeuAsn Ser Leu Gly Gly Gly Thr Ser Leu Gln Ile Thr Ile Ile SerValArg Gln Gly Glu Leu Phe His Cys Gln Ser Pro GlnAsn Gly GluAla Leu Ser Gln Leu Cys Ser Gly Leu GlyAsp SerAsp Ala Glu Leu GlnAsn Ser Cys Arg Ile SerArg Pro Trp Ile Met Gly Ser Met Met Phe Gly Gly Leu Gly Gly Leu Gly Gly MetAla Gly Gly Leu Gly GlyAla Leu Gly Gly Leu Val Ser Ser Leu Gly Gly Leu Gly Gly Gly Leu His Gly Ser Gly Leu Gly Gly Gly LeuAla Gly Gly Leu Ser SerArg Leu Gly Ser Gly Leu Gly GlyAla Val Gly Cys Gly Leu Gly Gly Ser LeuArg Gly Gly MetAsnAla Met Lys Ala Ser Leu Val Leu Gly Ser Leu Leu Val Gly Val Leu Glu His Leu Met Gly Gly Gly Met Ser Arg Trp Arg Val Gly Leu Phe Ala Ile Lys His AlaAla Ser Leu Gly Thr SerPro TyrMet Gly SerPhe His Asp Thr Trp SerAla Ile Ser GlyAsn Gly Leu Ser GlnAla GluArg Cys Val Ser Gly Leu Gln Leu Arg Asn Asn Gly Leu Gln Gly Leu Ser GlyAla GlyAla Phe Asn Lys Leu Cys Ser Thr Leu Gly Met Ala Val Gly Gln Lys Ala Gly Leu Gln Arg Arg Ile Gln Arg Tyr Asn Phe Gly Ser Gln Arg SerAla Arg Leu Glu Arg Arg Arg Gly Val Gln Pro Thr Glu Gln AlaArgTrp GlyTrp Phe TrpAlaArg Lys LeuVal CysArg SerTyrThrThr SerAla Arg Thr SerAlaAlaAla Pro Val Thr Ser Trp Ile Arg Lys Ile Gly Trp Glu Trp Arg Lys Arg Leu Val Ser MetAsp Gln Tyr Pro Glu Leu Phe Lys Pro Glu Tyr Gln Lys Asp Gln Pro Val Trp Gln Thr Glu Gln Glu Tyr GluArg Asn Val Leu Arg Pro Lys Arg Trp Thr Ser Ala Asn Gln Leu Thr Met Glu Ser Thr Ile Gly Ser Met His Arg Phe Met Lys Ala Val Gly Ile Arg Gln Glu Arg Trp Ala Cys Glu Tyr Pro Cys Lys Ser Thr Ser Thr Leu Val Gly Thr GlyArgArg Phe Ala Gly Ile Asp AlaAla Ile Ile GlyAspArg IleValAsnMet GlyLeu Gln Lys Leu Ser Ser。
in the present invention, the HrpN isEccPRProtein HrpN disclosed by Chinese patent CN106867929A (Erwinia carotovora, plant immune activation protein secreted by Erwinia carotovora and application)EccPRThe protein is prepared by the preparation method.
The plant vegetable growth regulator provided by the invention comprises 90-95 parts by weight of water, preferably 91-94 parts by weight of water, and more preferably 92-93 parts by weight of water.
In the invention, the plant vegetable growth regulator also preferably comprises 5-8 parts of proteolytic amino acid, and more preferably 6-7 parts. The source of the proteolytic amino acid is not particularly limited, the proteolytic amino acid is commercially available and is generally adopted by a person skilled in the art, and the proteolytic amino acid sold by Yaji biotechnology limited in Shanghai is specifically purchased in the embodiment of the invention, the proteolytic amino acid contains 17 amino acids, and the effective component is more than or equal to 45%. In the present invention, the proteolytic amino acids preferably include lysine, arginine and histidine. In the present invention, the proteolytic amino acids can provide sufficient nutrients to vegetables to increase the HrpNEccPRPromoting vegetable growth.
The preparation method of the plant vegetable growth regulator is not particularly limited, and the plant vegetable growth regulator is preferably prepared by mixing the components.
The invention also provides application of the plant vegetable growth regulator in the technical scheme in improving the yield and/or quality of plant vegetables. In the present invention, the plant vegetable growth regulator can improve the yield and quality of plant vegetables.
In the present invention, the vegetable vegetables include leaf lettuce, cucumber, chinese cabbage, tomato, cabbage mustard, chili, pumpkin, carrot or soybean.
In the present invention, the plant vegetable growth regulator is preferably used in a method comprising: the plant vegetable growth regulator and vegetable seeds are mixed and soaked, and the mass ratio of the plant vegetable growth regulator to the vegetable seeds is preferably 1: 1000-3500, more preferably 1: 1500-3200, and most preferably 1: 2500-3000. In the invention, the seed soaking time is preferably 6-10 h, more preferably 7-9 h, and most preferably 8 h.
In the present invention, the application of the plant vegetable growth regulator preferably further comprises: after the plant vegetables grow seedlings, the plant vegetable growth regulator is preferably diluted by 500-1500 times and then sprayed. In the invention, the dilution multiple is more preferably 800-1200 times, and most preferably 1000 times; the amount of the plant vegetable growth regulator sprayed each time is preferably 0.5-0.7 g/m2More preferably 0.6g/m2。
The present invention will be described in detail with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
This example uses HrpNEccPRProtein as vegetable growth regulator, water as negative control, and plant enhancer with peptides as positive control, wherein HrpN is used per mu of landEccPRThe protein is 10 g/time, the peptide is 10 g/time, and the product is applied to the planting of the leaf lettuce.
The using method comprises the following steps: (1) HrpNEccPRProtein seed soaking: make itUsing 10gHrpNEccPRProtein preparation of 2% (mass to volume) HrpNEccPRProtein water solution, 500g of leaf lettuce seeds (the amount of leaf lettuce seeds used in one mu of land) are soaked in the protein water solution for 8 hours, and then the seeds are sowed after being drained; treating lettuce seeds with plant growth chemical regulator peptide by the same method; (2) spraying at the early seedling stage: mixing 10g of HrpNEccPRProtein (HrpN used once per mu of land)EccPRAmount of protein) preparation of 2% (mass to volume) HrpNEccPRProtein in water solution, then adding 2% HrpNEccPRDiluting the protein water solution 1000 times, and spraying HrpN to the leaf lettuce when the leaf lettuce seedlings are about 5cm away from the groundEccPRThe protein diluent is sprayed for the second time after 10 days for 1 time; the lettuce was treated by the same method with the plant growth chemical regulator, namely, pepstatin, diluted 1000 times, and the results are shown in Table 1.
TABLE 1 HrpNEccPREffect of protein on lettuce growth
As can be seen from Table 1, HrpN was usedEccPRCompared with water treatment after protein treatment, the yield of the leaf lettuce is increased by 39 percent, and compared with the plant growth chemical regulator peptide treatment, the yield of the leaf lettuce is increased by 26 percent. Using HrpNEccPRAfter the protein is added, the leaf of the leaf lettuce is neat, the leaf is thick and tender, the color is light green, the texture is crisp and tender, the taste is very fresh and tender, and no chemical harmful substance residues exist.
Example 2
The vegetable growth regulator used in the embodiment comprises the following components in parts by weight:
HrpNEccPR0.2g of protein, 5g of proteolytic amino acid and 94.8g of water. The vegetable growth regulator is applied to the planting of cucumbers. This example uses water as a negative control and 0.2% brassinolide (market-for-market) plant enhancer as a positive controlSexual control, the same method was applied to cucumber.
The using method comprises the following steps: (1) soaking the vegetable growth regulator: 0.05% HrpN was preparedEccPRSoaking cucumber seeds in the composite plant growth regulator aqueous solution with equal mass for 8 hours, draining, preserving moisture and accelerating germination at a constant temperature box of 28 ℃, starting germination within 20 hours, and then planting after seedbed seedling culture; diluting 0.2% brassinolide of plant growth regulator to 0.05%, and treating cucumber seed by the same method; (2) spraying at the early seedling stage: diluting the vegetable growth regulator of the embodiment by 1000 times, spraying the compound plant growth regulator to the cucumber for 1 time when the cucumber seedlings have 4 leaves, spraying the compound plant growth regulator for the second time after 10 days, and spraying the compound plant growth regulator for the third time after 20 days; the plant growth regulator 0.2% brassinolide was diluted to 0.05% and cucumber was treated in the same manner, and the results are shown in Table 2.
TABLE 2 Effect of HrpNECCPR protein Complex plant growth regulator on cucumber growth
| Name of preparation | Application dose (g/times/mu) | Number of times of use | Yield (kilogram/mu) | Less untreated yield (%) |
| Water (W) | 10 | 4 | 4978 | |
| Vegetable growth regulator | 10 | 4 | 6910 | 45％ |
| Brassinolide | 10 | 4 | 5724 | 15％ |
It can be seen from table 2 that the yield of cucumber was increased by 45% compared to water treated cucumber and 15% compared to brassinolide treated cucumber after using the vegetable growth regulator. After the vegetable growth regulator is used, the cucumber has no deformed cucumber, the plant has no premature senility, and the plant and cucumber have low susceptibility to diseases.
Example 3
The vegetable growth regulator used in the embodiment comprises the following components in parts by weight: HrpNEccPR0.2g of protein and 99.8g of water. The vegetable growth regulator is applied to planting of Chinese cabbage. This example used water as a negative control and 0.2% brassinolide plant enhancer as a positive control, applied to chinese cabbage in the same manner.
The using method comprises the following steps: diluting the vegetable growth regulator of the embodiment by 1000 times, spraying for 1 time after 1 month of planting the Chinese cabbage, and spraying for the second time after 15 days; after another 20 days, the spray is sprayed for the third time. The results of diluting 0.1% brassinolide as a plant growth regulator to 0.05% and treating the celery cabbage by the same method are shown in tables 3-4.
TABLE 3 Effect of vegetable growth regulators on the growth of Chinese cabbage
| Treatment of | Single fresh weight (kilogram) | Yield per mu (kilogram) | Increase the yield |
| Water (W) | 2.14e | 5345e | |
| Vegetable growth regulator | 3.29a | 8234a | 54.05 |
| Brassinolide | 2.64 | 6601b | 23.5％ |
As can be seen from Table 3, the yield of Chinese cabbage increased 54.05% compared to water treatment and 30.55% compared to brassinolide treatment after using the vegetable growth regulator.
TABLE 4 influence of vegetable growth regulators on the quality of Chinese cabbage
As can be seen from Table 4, after the vegetable growth regulator is used, the leaf of the Chinese cabbage is enlarged, the leaf is thickened, the leaf has more folds, the vegetable stem is small, the leaf tendon is less, the water content of the vegetable is less, the leaf ball is light green, the middle pile is overlapped, the ball height is 42.5cm, the ball width is 19.2cm, the heart fullness reaches 97.32%, and the difference of the brassinolide treated Chinese cabbage compared with the vegetable growth regulator is less.
Example 4
The vegetable growth regulator used in the embodiment comprises the following components in parts by weight:
HrpNEccPR0.2g of protein, 5g of proteolytic amino acid and 94.8g of water. The vegetable growth regulator is applied to planting of tomatoes. This example uses water as a negative control and 0.2% brassinolide (commercially available) plant enhancer as a positive control, and is applied to tomato in the same manner.
The using method comprises the following steps: (1) soaking the vegetable growth regulator: 0.05% HrpN was preparedEccPRSoaking tomato seeds in the composite plant growth regulator aqueous solution with equal mass for 8 hours, draining, preserving moisture and accelerating germination at a constant temperature box of 28 ℃, starting germination within 20 hours, and then planting after seedbed seedling culture; diluting plant growth regulator 0.2% brassinolide to 0.05%, and treating tomato seed by the same method; (2) spraying at the early seedling stage: diluting the vegetable growth regulator of the embodiment by 1000 times, spraying the compound plant growth regulator to the tomatoes for 1 time when the tomatoes have 4 leaves in the initial seedling, spraying the compound plant growth regulator for the second time after 10 days, and spraying the compound plant growth regulator for the third time after 20 days; the fourth spray before tomato flowering, plant growth regulator 0.2% brassinolide diluted to 0.05%, tomato treated in the same way, the results are shown in table 5.
TABLE 5 Effect of HrpNECCPR protein Complex plant growth regulator on tomato growth
| Name of preparation | Application dose (g/times/mu) | Number of times of use | Yield (kilogram/mu) | Less untreated yield (%) |
| Water (W) | 10 | 5 | 3985 | |
| Vegetable growth regulator | 10 | 5 | 5420 | 36％ |
| Brassinolide | 10 | 5 | 4423 | 11％ |
It can be seen from table 5 that the tomato yield increased by 36% compared to water treatment, whereas the brassinolide treatment increased by 11% after the use of the vegetable growth regulator. After the vegetable growth regulator is used, the tomato has no malformed fruit, the plant has no premature senility, and the plant and the tomato have low susceptibility.
Example 5
The vegetable growth regulator used in the embodiment comprises the following components in parts by weight:
HrpNEccPRprotein 0.3g, protein water4g of amino acid and 95.7g of water. The vegetable growth regulator is applied to planting of cabbage mustard. This example uses water as a negative control and 0.2% brassinolide (commercially available) plant enhancer as a positive control, and the same method is applied to cabbage mustard.
The using method comprises the following steps: (1) soaking the vegetable growth regulator: 0.05% HrpN was preparedEccPRSoaking cabbage mustard seeds in the composite plant growth regulator aqueous solution with equal quality for 8 hours, draining, preserving moisture and accelerating germination at a thermostat of 28 ℃, starting germination within 20 hours, and then planting after seedbed seedling culture; diluting 0.2% brassinolide as plant growth regulator to 0.05%, and treating cabbage mustard seed by the same method; (2) spraying at the early seedling stage: diluting the vegetable growth regulator of the embodiment by 1000 times, spraying the compound type plant growth regulator to the cabbage mustard for 1 time when the cabbage mustard is initially planted for 5cm, spraying the compound type plant growth regulator for the second time after 10 days, and spraying the compound type plant growth regulator for the third time after 20 days; the plant growth regulator, 0.2% brassinolide, was diluted to 0.05% and cabbage mustard was treated in the same manner, with the results shown in Table 6.
TABLE 6 Effect of HrpNECCPR protein Complex plant growth regulator on the growth of cabbage mustard
| Name of preparation | Application dose (g/times/mu) | Number of times of use | Yield (kilogram/mu) | Less untreated yield (%) |
| Water (W) | 10 | 4 | 2314 | |
| Vegetable growth regulator | 10 | 4 | 3263 | 41％ |
| Brassinolide | 10 | 4 | 2499 | 8％ |
As can be seen from table 6, the yield of cabbage mustard increased 41% compared to water treatment, while the yield of cabbage mustard increased 8% compared to brassinolide treatment, using the vegetable growth regulator. After the vegetable growth regulator is used, the weight of a single plant is increased, the plant is higher, big and strong, and the growth is vigorous. The blade is nearly circular, the texture is thinner, and the wax powder is less. The leaf surface is slightly wrinkled, and the leaf edge is slightly bent inwards, and is shaped like a shell. The leaf base is deeply split into ear-shaped splinters. The main flower has crisp and tender tongue coating, less fiber, strong germination of lateral flowering branch and low susceptibility to diseases.
The embodiments show that the plant vegetable growth regulator provided by the invention improves the yield and quality of leaf lettuce, cucumber, Chinese cabbage, tomato and cabbage mustard, and has no chemical harmful substance residue, no public nuisance and no pollution.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Sequence listing
<110> institute of biological research of academy of sciences of Hebei province
<120> a plant vegetable growth regulator and use
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 417
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 1
Met Leu Asn Ser Leu Gly Gly Gly Thr Ser Leu Gln Ile Thr Ile Ile
1 5 10 15
Ser Val Arg Gln Gly Glu Leu Phe His Cys Gln Ser Pro Gln Asn Gly
20 25 30
Glu Ala Leu Ser Gln Leu Cys Ser Gly Leu Gly Asp Ser Asp Ala Glu
35 40 45
Leu Gln Asn Ser Cys Arg Ile Ser Arg Pro Trp Ile Met Gly Ser Met
50 55 60
Met Phe Gly Gly Leu Gly Gly Leu Gly Gly Met Ala Gly Gly Leu Gly
65 70 75 80
Gly Ala Leu Gly Gly Leu Val Ser Ser Leu Gly Gly Leu Gly Gly Gly
85 90 95
Leu His Gly Ser Gly Leu Gly Gly Gly Leu Ala Gly Gly Leu Ser Ser
100 105 110
Arg Leu Gly Ser Gly Leu Gly Gly Ala Val Gly Cys Gly Leu Gly Gly
115 120 125
Ser Leu Arg Gly Gly Met Asn Ala Met Lys Ala Ser Leu Val Leu Gly
130 135 140
Ser Leu Leu Val Gly Val Leu Glu His Leu Met Gly Gly Gly Met Ser
145 150 155 160
Arg Trp Arg Val Gly Leu Phe Ala Ile Lys His Ala Ala Ser Leu Gly
165 170 175
Thr Ser Pro Tyr Met Gly Ser Phe His Asp Thr Trp Ser Ala Ile Ser
180 185 190
Gly Asn Gly Leu Ser Gln Ala Glu Arg Cys Val Ser Gly Leu Gln Leu
195 200 205
Arg Asn Asn Gly Leu Gln Gly Leu Ser Gly Ala Gly Ala Phe Asn Lys
210 215 220
Leu Cys Ser Thr Leu Gly Met Ala Val Gly Gln Lys Ala Gly Leu Gln
225 230 235 240
Arg Arg Ile Gln Arg Tyr Asn Phe Gly Ser Gln Arg Ser Ala Arg Leu
245 250 255
Glu Arg Arg Arg Gly Val Gln Pro Thr Glu Gln Ala Arg Trp Gly Trp
260 265 270
Phe Trp Ala Arg Lys Leu Val Cys Arg Ser Tyr Thr Thr Ser Ala Arg
275 280 285
Thr Ser Ala Ala Ala Pro Val Thr Ser Trp Ile Arg Lys Ile Gly Trp
290 295 300
Glu Trp Arg Lys Arg Leu Val Ser Met Asp Gln Tyr Pro Glu Leu Phe
305 310 315 320
Lys Pro Glu Tyr Gln Lys Asp Gln Pro Val Trp Gln Thr Glu Gln Glu
325 330 335
Tyr Glu Arg Asn Val Leu Arg Pro Lys Arg Trp Thr Ser Ala Asn Gln
340 345 350
Leu Thr Met Glu Ser Thr Ile Gly Ser Met His Arg Phe Met Lys Ala
355 360 365
Val Gly Ile Arg Gln Glu Arg Trp Ala Cys Glu Tyr Pro Cys Lys Ser
370 375 380
Thr Ser Thr Leu Val Gly Thr Gly Arg Arg Phe Ala Gly Ile Asp Ala
385 390 395 400
Ala Ile Ile Gly Asp Arg Ile Val Asn Met Gly Leu Gln Lys Leu Ser
405 410 415
Ser
Claims (4)
1. The application of the plant vegetable growth regulator in improving the yield of plant vegetables; the plant growth regulator comprises the following components in parts by weight: 0.1-0.5 part of HrpNECcPR protein and 90-95 parts of water; the amino acid sequence of the HrpNECcPR protein is shown as SEQ ID No. 1; the vegetable can be leaf lettuce, cucumber, Chinese cabbage, tomato or cabbage mustard.
2. Use of a plant-based vegetable growth regulator according to claim 1 for increasing yield of a plant-based vegetable, further comprising: 5-8 parts of proteolytic amino acid.
3. Use of a plant vegetable growth regulator according to claim 1 for increasing the yield of a plant vegetable, wherein said use is: and mixing the plant vegetable growth regulator with seeds of plant vegetables for seed soaking, wherein the mass ratio of the plant vegetable growth regulator to the seeds of the plant vegetables is 1: 1000-3500.
4. Use of a plant vegetable growth regulator according to claim 1 for increasing the yield of a plant vegetable, wherein said use is: and (3) diluting the plant vegetable growth regulator by 500-1500 times, and spraying the plant vegetable growth regulator to plant vegetables, wherein the amount of the plant vegetable growth regulator sprayed each time is 2-30 g/mu.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810175156.1A CN108308181B (en) | 2018-03-02 | 2018-03-02 | Plant vegetable growth regulator and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810175156.1A CN108308181B (en) | 2018-03-02 | 2018-03-02 | Plant vegetable growth regulator and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108308181A CN108308181A (en) | 2018-07-24 |
| CN108308181B true CN108308181B (en) | 2021-06-29 |
Family
ID=62901172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810175156.1A Active CN108308181B (en) | 2018-03-02 | 2018-03-02 | Plant vegetable growth regulator and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108308181B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106867929A (en) * | 2016-12-21 | 2017-06-20 | 河北省科学院生物研究所 | A kind of carrot soft rot Erwinia, the plant immune activator protein of its secretion and application |
-
2018
- 2018-03-02 CN CN201810175156.1A patent/CN108308181B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106867929A (en) * | 2016-12-21 | 2017-06-20 | 河北省科学院生物研究所 | A kind of carrot soft rot Erwinia, the plant immune activator protein of its secretion and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108308181A (en) | 2018-07-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zodape | Seaweeds as a biofertilizer | |
| Fawzy et al. | Influence of foliar application by EM" effective microorganisms", amino acids and yeast on growth, yield and quality of two cultivars of onion plants under newly reclaimed soil | |
| Reitz et al. | Effects of cytokinin-containing seaweed extract on Phaseolus lunatus L.: influence of nutrient availability and apex removal | |
| CN105613006A (en) | Peanut planting method | |
| Al-Madhagi | Effect of humic acid and yeast on the yield of greenhouse cucumber. | |
| El-Boray et al. | IMPROVING YIELD AND FRUIT QUALITY OF WASHINGTON NAVEL ORANGE USING FOLIAR APPLICATIONS OF SOME NATURAL BIOSTIMULANTS. | |
| Abdelkader et al. | The influence of biostimulants on tomato plants cultivated under hydroponic systems | |
| CN108308181B (en) | Plant vegetable growth regulator and application | |
| Abbas et al. | Physiological and biochemical responses of two cultivars of Phaseolus vulgaris L. to application of organic fertilizers and nile compost in sandy soil | |
| AU2020339071B2 (en) | Biostimulant agent for treating plants and/or plant seed | |
| JP4974213B2 (en) | Plant vitality agent | |
| Varfolomeyeva et al. | The effect of mineral fertilizers on the yield of vegetable beans, depending on the competition between plants in crops | |
| Al-Malieky et al. | Preparation protein hydrolysates from fish by-product and study effected on lettuce (Lactuca sativa L.) growth, yield, quality and enhanced salt tolerance | |
| JP2003073186A (en) | Natural amino acid fertilizer derived from silk protein and its production method | |
| KR101630361B1 (en) | Method of producing a plant containing a zinc component | |
| KR100451397B1 (en) | Bean sprorts having organic selenium | |
| Pasternak et al. | Moringa research and cultivation in Niger | |
| US6077811A (en) | Vanadium compositions to enhance plant growth and edible fruit yield | |
| JP7182228B2 (en) | Plant activator, production method thereof, fertilizer composition and plant growth method | |
| CN114174243B (en) | Composition for increasing plant nutrient content | |
| CN1196182A (en) | Bud preserving and fruit quick growth agent | |
| CN106561136A (en) | Planting method of zinc supplement chrysanthemum | |
| US5661105A (en) | Vanadium compositions to enhance plant growth and edible fruit yield | |
| JP2023036022A (en) | Plant activator, fertilizer composition, disease preventing method, and plant growing method | |
| Abd-Elatif et al. | Impact of some foliar application treatments on yield and quality of cassava in newly reclaimed soils |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |