CN108308181A - A kind of plant vegetable growth conditioning agent and application - Google Patents

A kind of plant vegetable growth conditioning agent and application Download PDF

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Publication number
CN108308181A
CN108308181A CN201810175156.1A CN201810175156A CN108308181A CN 108308181 A CN108308181 A CN 108308181A CN 201810175156 A CN201810175156 A CN 201810175156A CN 108308181 A CN108308181 A CN 108308181A
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plant
conditioning agent
gly
vegetable growth
ser
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CN108308181B (en
Inventor
贾振华
宋聪
郝少彦
宋水山
黄亚丽
黄媛媛
赵芊
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Institute of Biology of Hebei Academy of Sciences
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Institute of Biology of Hebei Academy of Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C11/00—Other nitrogenous fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
    • C05G5/23—Solutions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The present invention provides a kind of plant vegetable growth conditioning agent is provided, belongs to vegetable cultivation and technical field of cultivation, include the component of following parts by weight：HrpNEccPR0.1~0.5 part of albumen, 90~95 parts of water；The HrpNEccPRThe amino acid sequence of albumen is as shown in SEQ ID No.1.Plant vegetable growth conditioning agent provided by the invention is compared compared with water with plant growth regulator, and the yield and quality of Lettuce, cucumber, Chinese cabbage, tomato and cabbage mustard is improved, and is remained without chemical noxious substance, is nuisanceless, pollution-free.

Description

A kind of plant vegetable growth conditioning agent and application
Technical field
The invention belongs to vegetable cultivation and technical field of cultivation more particularly to a kind of plant vegetable growth conditioning agent and answer With.
Background technology
Vegetables are one of essential food in people's diet, and vegetables can provide a variety of dimension lifes necessary to human body The nutriments such as element and minerals, can effectively prevent chronic, degenerative disease, unite according to international substance food and agricultural organization nineteen ninety Meter, the 90% of VC needed by human, the 60% of VA comes from vegetables.Therefore, the public demand to vegetables dishes is increasing.
It is mainly at present chemicals and hormone product applied to the substance in terms of vegetable increase production, such as triacontanol, Huang Rotten acid, urea etc., such product effect of increasing production is between 10%~30%.There are chemical residual after existing use, to product and Environment can all cause different degrees of pollution, also adversely affected to the quality of product, do not meet the requirement of pollution-free food production.
Invention content
The purpose of the present invention is to provide a kind of plant vegetable growth conditioning agents, can improve the production of plant vegetables Amount, and nontoxic, pollution-free, noresidue, meet the requirement of pollution-free food.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：
The present invention provides a kind of plant vegetable growth conditioning agents, include the component of following parts by weight：
HrpNEccPR0.1~0.5 part of albumen, 90~95 parts of water；The HrpNEccPRThe amino acid sequence of albumen such as SEQ ID Shown in No.1.
Preferably, further include：5~8 parts of protein hydrolytic amino acid.
The present invention also provides the plant vegetable growth conditioning agents described in above-mentioned technical proposal to improve plant vegetables Application in yield and/or quality.
Preferably, the plant vegetables include Lettuce, cucumber, Chinese cabbage, tomato, cabbage mustard, capsicum, cucurbita pepo, Hu Radish or soybean.
Preferably, the application is：The plant vegetable growth conditioning agent is mixed into leaching with the seed of plant vegetables Kind, the mass ratio of the plant vegetable growth conditioning agent and the seed of plant vegetables is 1:1000~3500.
Preferably, the application is：The plant vegetable growth is adjusted into 500~1500 times of spray on plants classes of dilution agent Vegetables, the amount that the plant vegetable growth conditioning agent sprays every time are 2~30g/ mus.
The present invention provides a kind of plant vegetable growth conditioning agents, include the component of following parts by weight：HrpNEccPREgg White 0.1~0.5 part, 90~95 parts of water；The HrpNEccPRThe amino acid sequence of albumen is as shown in SEQ ID No.1.The present invention carries HrpN in the plant vegetable growth conditioning agent of confessionEccPRAlbumen can increase absorption of the vegetables to ASI systematic approach, to planting Species vegetable growth has good facilitation, and then can improve the yield of plant vegetables.
The result of the embodiment of the present invention is shown：Plant vegetable growth conditioning agent provided by the invention is compared with water and plant growth Conditioning agent is compared, and improves the yield and quality of Lettuce, cucumber, Chinese cabbage, tomato and cabbage mustard, and without chemical noxious substance It remains, is nuisanceless, pollution-free.
Specific implementation mode
The present invention provides a kind of plant vegetable growth conditioning agents, include the component of following parts by weight：HrpNEccPREgg White 0.1~0.5 part, 90~95 parts of water；The HrpNEccPRThe amino acid sequence of albumen is as shown in SEQ IDNo.1.
Plant vegetable growth conditioning agent provided by the invention includes the HrpN that parts by weight are 0.1~0.5 partEccPREgg In vain, preferably 0.2~0.4 part.In the present invention, the HrpNEccPRAlbumen can enhance the resistivity of plant vegetables, energy Enough increase absorption of the plant vegetables to ASI systematic approach, there is good facilitation to plant vegetable growth, in turn The yield of plant vegetables can be improved.Pass through HrpNEccPRAlbumen handle different crops or vegetables experiment, find its Role is different under various concentration, when its concentration is more than 1%, primarily serves the preventive and therapeutic effect of disease, and If be less than 0.1% using concentration, there is certain growth promoting function to plant.
In the present invention, the HrpNEccPRThe amino acid sequence of albumen is specific as follows as shown in SEQ ID No.1 It is shown：
Met LeuAsn Ser Leu Gly Gly Gly Thr Ser Leu Gln Ile Thr Ile Ile SerValArg Gln Gly Glu Leu Phe His Cys Gln Ser Pro GlnAsn Gly GluAla Leu Ser Gln Leu Cys Ser Gly Leu GlyAsp SerAsp Ala Glu Leu GlnAsn Ser Cys Arg Ile SerArg Pro Trp Ile Met Gly Ser Met Met Phe Gly Gly Leu Gly Gly Leu Gly Gly MetAla Gly Gly Leu Gly GlyAla Leu Gly Gly Leu Val Ser Ser Leu Gly Gly Leu Gly Gly Gly Leu His Gly Ser Gly Leu Gly Gly Gly LeuAla Gly Gly Leu Ser SerArg Leu Gly Ser Gly Leu Gly GlyAla Val Gly Cys Gly Leu Gly Gly Ser LeuArg Gly Gly MetAsnAla Met Lys Ala Ser Leu Val Leu Gly Ser Leu Leu Val Gly Val Leu Glu His Leu Met Gly Gly Gly Met Ser Arg Trp Arg Val Gly Leu Phe Ala Ile Lys His AlaAla Ser Leu Gly Thr SerPro TyrMet Gly SerPhe His Asp Thr Trp SerAla Ile Ser GlyAsn Gly Leu Ser GlnAla GluArg Cys Val Ser Gly Leu Gln Leu Arg Asn Asn Gly Leu Gln Gly Leu Ser GlyAla GlyAla Phe Asn Lys Leu Cys Ser Thr Leu Gly Met Ala Val Gly Gln Lys Ala Gly Leu Gln Arg Arg Ile Gln Arg Tyr Asn Phe Gly Ser Gln Arg SerAla Arg Leu Glu Arg Arg Arg Gly Val Gln Pro Thr Glu Gln AlaArgTrp GlyTrp Phe TrpAlaArg Lys LeuVal CysArg SerTyrThrThr SerAla Arg Thr SerAlaAlaAla Pro Val Thr Ser Trp Ile Arg Lys Ile Gly Trp Glu Trp Arg Lys Arg Leu Val Ser MetAsp Gln Tyr Pro Glu Leu Phe Lys Pro Glu Tyr Gln Lys Asp Gln Pro Val Trp Gln Thr Glu Gln Glu Tyr GluArg Asn Val Leu Arg Pro Lys Arg Trp Thr Ser Ala Asn Gln Leu Thr Met Glu Ser Thr Ile Gly Ser Met His Arg Phe Met Lys Ala Val Gly Ile Arg Gln Glu Arg Trp Ala Cys Glu Tyr Pro Cys Lys Ser Thr Ser Thr Leu Val Gly Thr GlyArgArg Phe Ala Gly Ile Asp AlaAla Ile Ile GlyAspArg IleValAsnMet GlyLeu Gln Lys Leu Ser Ser。
In the present invention, the HrpNEccPRAlbumen is according to a kind of Chinese patent CN106867929A (carrot soft rot Irvings Salmonella, its secretion plant immune activator protein and application) disclosed in HrpNEccPRThe preparation method of albumen is prepared.
It is 90~95 parts of water that plant vegetable growth conditioning agent provided by the invention, which includes parts by weight, preferably 91~ 94 parts, more preferably 92~93 parts.
In the present invention, the plant vegetable growth conditioning agent preferably further includes 5~8 parts of protein hydrolytic amino acid, More preferably 6~7 parts.The present invention is not particularly limited the source of the protein hydrolytic amino acid, using people in the art The commercial protein hydrolysis amino acid that member routinely uses, specific purchase is in Shanghai Ya Ji biotechnologies in embodiments of the present invention The protein hydrolytic amino acid of Co., Ltd's sale, the protein hydrolytic amino acid contain 17 kinds of amino acid, active ingredient >=45%. In the present invention, the protein hydrolytic amino acid preferably includes lysine, arginine and histidine.In the present invention, the egg White hydrolysis amino acid is capable of providing the enough nutrients of vegetables, increases HrpNEccPRPromote the effect of vegetable growth.
The present invention is not particularly limited the preparation method of the plant vegetable growth conditioning agent, preferably by each component Mixing.
The present invention also provides the plant vegetable growth conditioning agents described in above-mentioned technical proposal to improve plant vegetables Application in yield and/or quality.In the present invention, the plant vegetable growth conditioning agent can improve plant vegetables Yield and quality.
In the present invention, the plant vegetables include Lettuce, cucumber, Chinese cabbage, tomato, cabbage mustard, capsicum, western calabash Reed, carrot or soybean.
In the present invention, the application method of the plant vegetable growth conditioning agent is preferably：By plant vegetable growth Conditioning agent mixes seed soaking with vegetable seeds, and the mass ratio of the plant vegetable growth conditioning agent and vegetable seeds is preferably 1: 1000~3500, more preferably 1:1500~3200, most preferably 1:2500~3000.In the present invention, the seed soaking when Between preferably 6~10h, more preferably 7~9h, most preferably 8h.
In the present invention, the application of the plant vegetable growth conditioning agent preferably further includes：Wait for that plant vegetables emerge Afterwards, it will be sprayed after preferred 500~1500 times of the dilution of plant vegetable growth conditioning agent.In the present invention, described diluted Multiple is more preferably 800~1200 times, most preferably dilutes 1000 times；What the plant vegetable growth conditioning agent sprayed every time Amount is preferably 0.5~0.7g/m2, more preferably 0.6g/m2。
A kind of plant vegetable growth conditioning agent provided by the invention and application are carried out with reference to embodiment detailed Illustrate, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
The present embodiment uses HrpNEccPRAlbumen is as vegetable growth conditioning agent, using water as negative control, uses precious peptide Plant reinforcing agent is as positive control, wherein using HrpN per acreEccPRProtein 10 g/ times, precious peptide 10g/ times answer the product Plantation for Lettuce.
Application method：(1)HrpNEccPRAlbumen is soaked seed：Use 10gHrpNEccPRAlbumen prepares 2% (mass volume ratio) HrpNEccPRIt is water-soluble to be immersed in the albumen by protein solution for 500g Lettuces seed (the Lettuce grain weight that 0.07 hectare uses) In liquid, after impregnating 8 hours, sowed after leaching is dry；Plant growth chemical regulator treasure's peptide is handled into Lettuce using same method Seed；(2) first seedling spraying：By 10gHrpNEccPRAlbumen (0.07 hectare first use HrpNEccPRThe amount of albumen) prepare 2% (matter Measure volume ratio) HrpNEccPRProtein solution, then by 2%HrpNEccPRProtein solution dilutes 1000 times, at the beginning of Lettuce HrpN is sprayed to Lettuce when seedling is liftoff 5cm or soEccPRIt is sprayed after diluted protein solution 1 time, 10 days second；By plant growth It learns conditioning agent treasure's peptide and dilutes the same method processing Lettuce of 1000 times of utilizations, the results are shown in Table 1.
1 HrpN of tableEccPRThe influence that albumen grows Lettuce
As can be drawn from Table 1, using HrpNEccPRIncrease by 39% compared with water process, Lettuce yield after albumen, is given birth to compared with plant Long chemical regulator treasure peptide processing, the yield of Lettuce increase by 26%.Use HrpNEccPRAfter albumen, Lettuce gesture is neat, leaf Piece thickness is tender, and color and luster pale green, quality is tender and crisp, and mouthfeel is extremely fresh and tender, no chemical noxious substance residue.
Embodiment 2
The each group distribution ratio for the vegetable growth conditioning agent that the present embodiment uses is as follows：
HrpNEccPRAlbumen 0.2g, protein hydrolytic amino acid 5g, water 94.8g.The vegetable growth conditioning agent is applied to cucumber Plantation.The present embodiment uses water as negative control, using 0.2% brassin lactones (purchase market) plant reinforcing agent as Positive control, same method are administered on cucumber.
Application method：(1) vegetable growth conditioning agent is soaked seed：Prepare 0.05% HrpNEccPRProtein composition type plant growth Cucumber seeds are immersed in etc. in the composite plant growth regulator aqueous solution of quality by conditioning agent aqueous solution, are impregnated 8 hours Afterwards, flood rises and drains, and the moisturizing vernalization at 28 DEG C of insulating box, 20 hours start to germinate, and are planted again after the nursery of seedbed；Plant is given birth to 0.2% brassin lactones of long conditioning agent are diluted to 0.05%, and cucumber seeds are handled using same method；(2) first seedling spraying： The present embodiment vegetable growth is adjusted into 1000 times of dilution agent, composite plant growth is sprayed to cucumber in 4 leaves of seedling at the beginning of cucumber It is sprayed after conditioning agent 1 time, 10 days second, third time is sprayed after 20 days；0.2% brassin lactones of plant growth regulator are dilute It releases to 0.05%, handles cucumber using same method, the results are shown in Table 2.
Influence of the 2 HrpNEccPR protein composition types plant growth regulator of table to cucumber growth
Preparation title Dosage (g/ times/mu) Access times Yield (kg/acre) The volume increase (%) less handled
Water 10 4 4978
Vegetable growth conditioning agent 10 4 6910 45%
Brassin lactones 10 4 5724 15%
As can be drawn from Table 2, after using vegetable growth conditioning agent, increase by 45% compared with water process cucumber yield, compared with brassin Lactone processing, cucumber yield increase by 15%.After vegetable growth conditioning agent, cucumber without lopsided melon, plant without early ageing, plant and Cucumber is susceptible low.
Embodiment 3
The each group distribution ratio for the vegetable growth conditioning agent that the present embodiment uses is：HrpNEccPRAlbumen 0.2g, water 99.8g.It will The vegetable growth conditioning agent is applied to the plantation of Chinese cabbage.The present embodiment uses water as negative control, uses 0.2% brassin Lactone plant reinforcing agent is administered to as positive control, same method on Chinese cabbage.
Application method：The present embodiment vegetable growth is adjusted into 1000 times of dilution agent, is sprayed 1 time after Chinese cabbage is planted 1 month, It is sprayed after 15 days second；Third time is sprayed after 20 days.0.1% brassin lactones of plant growth regulator are diluted to 0.05%, Chinese cabbage is handled using same method, the results are shown in Table 3~4.
Influence of the 3 vegetable growth conditioning agent of table to Chinese Cabbage
Processing Single fresh weight (kilogram) Per mu yield (kilogram) Increase production %
Water 2.14e 5345e
Vegetable growth conditioning agent 3.29a 8234a 54.05
Brassin lactones 2.64 6601b 23.5%
As can be drawn from Table 3, after using vegetable growth conditioning agent, compared with water process, Chinese Cabbage Yield increases by 54.05%, compared with Brassin lactones processing, Chinese Cabbage Yield increase 30.55%.
Influence of the 4 vegetable growth conditioning agent of table to Chinese cabbage quality
As can be drawn from Table 4, after using vegetable growth conditioning agent, cabbage leaves increase, and leaf thickens, blade fold More, dish stalk is small, and ribs of a leaf are few, and the water content of dish is less, and leaf-head is in light green, and middle stake is folded to embrace, the high 42.5cm of ball, and ball is wide 19.2cm, cabbage heart plumpness reach 97.32%, and the Chinese cabbage of brassin lactones processing wants poor compared with what vegetable growth conditioning agent was handled Some.
Embodiment 4
The each group distribution ratio for the vegetable growth conditioning agent that the present embodiment uses is as follows：
HrpNEccPRAlbumen 0.2g, protein hydrolytic amino acid 5g, water 94.8g.The vegetable growth conditioning agent is applied to tomato Plantation.The present embodiment uses water as negative control, using 0.2% brassin lactones (purchase market) plant reinforcing agent as Positive control, same method are administered on tomato.
Application method：(1) vegetable growth conditioning agent is soaked seed：Prepare 0.05% HrpNEccPRProtein composition type plant growth Tomato seeds are immersed in etc. in the composite plant growth regulator aqueous solution of quality by conditioning agent aqueous solution, are impregnated 8 hours Afterwards, flood rises and drains, and the moisturizing vernalization at 28 DEG C of insulating box, 20 hours start to germinate, and are planted again after the nursery of seedbed；Plant is given birth to 0.2% brassin lactones of long conditioning agent are diluted to 0.05%, and tomato seeds are handled using same method；(2) first seedling spraying： The present embodiment vegetable growth is adjusted into 1000 times of dilution agent, composite plant growth is sprayed to tomato in 4 leaves of seedling at the beginning of tomato It is sprayed after conditioning agent 1 time, 10 days second, third time is sprayed after 20 days；It is sprayed before tomato blooms the 4th time, plant is given birth to 0.2% brassin lactones of long conditioning agent are diluted to 0.05%, handle tomato using same method, the results are shown in Table 5.
The influence of 5 HrpNEccPR protein composition type Plant Growth Regulators on Tomato of table growth
Preparation title Dosage (g/ times/mu) Access times Yield (kg/acre) The volume increase (%) less handled
Water 10 5 3985
Vegetable growth conditioning agent 10 5 5420 36%
Brassin lactones 10 5 4423 11%
As can be drawn from Table 5, after using vegetable growth conditioning agent, increase by 36% compared with water process tomato yield, and brassin Lactone processing, tomato yield increase by 11%.After vegetable growth conditioning agent, tomato without malformed fruit, plant without early ageing, plant and Tomato is susceptible low.
Embodiment 5
The each group distribution ratio for the vegetable growth conditioning agent that the present embodiment uses is as follows：
HrpNEccPRAlbumen 0.3g, protein hydrolytic amino acid 4g, water 95.7g.The vegetable growth conditioning agent is applied to cabbage mustard Plantation.The present embodiment uses water as negative control, using 0.2% brassin lactones (purchase market) plant reinforcing agent as Positive control, same method are administered on cabbage mustard.
Application method：(1) vegetable growth conditioning agent is soaked seed：Prepare 0.05% HrpNEccPRProtein composition type plant growth Cabbage mustard seed is immersed in etc. in the composite plant growth regulator aqueous solution of quality by conditioning agent aqueous solution, is impregnated 8 hours Afterwards, flood rises and drains, and the moisturizing vernalization at 28 DEG C of insulating box, 20 hours start to germinate, and are planted again after the nursery of seedbed；Plant is given birth to 0.2% brassin lactones of long conditioning agent are diluted to 0.05%, and cabbage mustard seed is handled using same method；(2) first seedling spraying： The present embodiment vegetable growth is adjusted into 1000 times of dilution agent, composite plant growth is sprayed to cabbage mustard in 5 centimetres of seedling at the beginning of cabbage mustard It is sprayed after conditioning agent 1 time, 10 days second, third time is sprayed after 20 days；0.2% brassin lactones of plant growth regulator are dilute It releases to 0.05%, handles cabbage mustard using same method, the results are shown in Table 6.
The influence that 6 HrpNEccPR protein composition types plant growth regulator of table grows cabbage mustard
Preparation title Dosage (g/ times/mu) Access times Yield (kg/acre) The volume increase (%) less handled
Water 10 4 2314
Vegetable growth conditioning agent 10 4 3263 41%
Brassin lactones 10 4 2499 8%
As can be drawn from Table 6, after using vegetable growth conditioning agent, increase by 41% compared with water process cabbage mustard yield, and brassin Lactone processing, cabbage mustard yield increase by 8%.After vegetable growth conditioning agent, single plant weight increases, and plant is taller and bigger sturdy, raw Length is vigorous.Blade subcircular, quality is relatively thin, and wax powder is few.Blade face is slightly wrinkled, and leaf margin is slightly inwardly curved, shaped like shelly-shaped.Leaf base drastic crack at Ear sliver.Main flower tongue matter is tender and crisp, and few fiber, side spray germination is strong, susceptible low.
As seen from the above embodiment, plant vegetable growth conditioning agent provided by the invention improves Lettuce, cucumber, big The yield and quality of Chinese cabbage, tomato and cabbage mustard, and without chemical noxious substance residual, it is nuisanceless, pollution-free.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.
Sequence table
<110>Biology Inst., Hebei Academy of Sciences
<120>A kind of plant vegetable growth conditioning agent and application
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 417
<212> PRT
<213>Artificial sequence (Artificial Sequence)
<400> 1
Met Leu Asn Ser Leu Gly Gly Gly Thr Ser Leu Gln Ile Thr Ile Ile
1 5 10 15
Ser Val Arg Gln Gly Glu Leu Phe His Cys Gln Ser Pro Gln Asn Gly
20 25 30
Glu Ala Leu Ser Gln Leu Cys Ser Gly Leu Gly Asp Ser Asp Ala Glu
35 40 45
Leu Gln Asn Ser Cys Arg Ile Ser Arg Pro Trp Ile Met Gly Ser Met
50 55 60
Met Phe Gly Gly Leu Gly Gly Leu Gly Gly Met Ala Gly Gly Leu Gly
65 70 75 80
Gly Ala Leu Gly Gly Leu Val Ser Ser Leu Gly Gly Leu Gly Gly Gly
85 90 95
Leu His Gly Ser Gly Leu Gly Gly Gly Leu Ala Gly Gly Leu Ser Ser
100 105 110
Arg Leu Gly Ser Gly Leu Gly Gly Ala Val Gly Cys Gly Leu Gly Gly
115 120 125
Ser Leu Arg Gly Gly Met Asn Ala Met Lys Ala Ser Leu Val Leu Gly
130 135 140
Ser Leu Leu Val Gly Val Leu Glu His Leu Met Gly Gly Gly Met Ser
145 150 155 160
Arg Trp Arg Val Gly Leu Phe Ala Ile Lys His Ala Ala Ser Leu Gly
165 170 175
Thr Ser Pro Tyr Met Gly Ser Phe His Asp Thr Trp Ser Ala Ile Ser
180 185 190
Gly Asn Gly Leu Ser Gln Ala Glu Arg Cys Val Ser Gly Leu Gln Leu
195 200 205
Arg Asn Asn Gly Leu Gln Gly Leu Ser Gly Ala Gly Ala Phe Asn Lys
210 215 220
Leu Cys Ser Thr Leu Gly Met Ala Val Gly Gln Lys Ala Gly Leu Gln
225 230 235 240
Arg Arg Ile Gln Arg Tyr Asn Phe Gly Ser Gln Arg Ser Ala Arg Leu
245 250 255
Glu Arg Arg Arg Gly Val Gln Pro Thr Glu Gln Ala Arg Trp Gly Trp
260 265 270
Phe Trp Ala Arg Lys Leu Val Cys Arg Ser Tyr Thr Thr Ser Ala Arg
275 280 285
Thr Ser Ala Ala Ala Pro Val Thr Ser Trp Ile Arg Lys Ile Gly Trp
290 295 300
Glu Trp Arg Lys Arg Leu Val Ser Met Asp Gln Tyr Pro Glu Leu Phe
305 310 315 320
Lys Pro Glu Tyr Gln Lys Asp Gln Pro Val Trp Gln Thr Glu Gln Glu
325 330 335
Tyr Glu Arg Asn Val Leu Arg Pro Lys Arg Trp Thr Ser Ala Asn Gln
340 345 350
Leu Thr Met Glu Ser Thr Ile Gly Ser Met His Arg Phe Met Lys Ala
355 360 365
Val Gly Ile Arg Gln Glu Arg Trp Ala Cys Glu Tyr Pro Cys Lys Ser
370 375 380
Thr Ser Thr Leu Val Gly Thr Gly Arg Arg Phe Ala Gly Ile Asp Ala
385 390 395 400
Ala Ile Ile Gly Asp Arg Ile Val Asn Met Gly Leu Gln Lys Leu Ser
405 410 415
Ser

Claims (6)

1. a kind of plant vegetable growth conditioning agent, includes the component of following parts by weight：
HrpNEccPR0.1~0.5 part of albumen, 90~95 parts of water；The HrpNEccPRThe amino acid sequence of albumen such as SEQ ID No.1 It is shown.
2. plant vegetable growth conditioning agent according to claim 1, which is characterized in that further include：Proteolysis amino 5~8 parts of acid.
3. plant vegetable growth conditioning agent as claimed in claim 1 or 2 is in improving plant yield of vegetables and/or quality Using.
4. application according to claim 3, which is characterized in that the plant vegetables include Lettuce, cucumber, great Bai Dish, tomato, cabbage mustard, capsicum, cucurbita pepo, carrot or soybean.
5. application according to claim 4, which is characterized in that the application is：The plant vegetable growth is adjusted Agent mixes seed soaking, the quality of the plant vegetable growth conditioning agent and the seed of plant vegetables with the seed of plant vegetables Than being 1:1000~3500.
6. application according to claim 4, which is characterized in that the application is：The plant vegetable growth is adjusted 500~1500 times of spray on plants class vegetables of dilution agent, the amount that the plant vegetable growth conditioning agent sprays every time are 2~30g/ Mu.
CN201810175156.1A 2018-03-02 2018-03-02 Plant vegetable growth regulator and application Active CN108308181B (en)

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