CN108294224A - A method of it is fermented using lactobacillus plantarum and removes heavy metal cadmium in rice - Google Patents
A method of it is fermented using lactobacillus plantarum and removes heavy metal cadmium in rice Download PDFInfo
- Publication number
- CN108294224A CN108294224A CN201810033884.9A CN201810033884A CN108294224A CN 108294224 A CN108294224 A CN 108294224A CN 201810033884 A CN201810033884 A CN 201810033884A CN 108294224 A CN108294224 A CN 108294224A
- Authority
- CN
- China
- Prior art keywords
- rice
- cadmium
- fermentation
- lactobacillus plantarum
- bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 109
- 235000009566 rice Nutrition 0.000 title claims abstract description 109
- 229910052793 cadmium Inorganic materials 0.000 title claims abstract description 81
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 40
- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 22
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 22
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 22
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 15
- 240000007594 Oryza sativa Species 0.000 title description 2
- 241000209094 Oryza Species 0.000 claims abstract description 108
- 238000000855 fermentation Methods 0.000 claims abstract description 63
- 230000004151 fermentation Effects 0.000 claims abstract description 63
- 241000894006 Bacteria Species 0.000 claims abstract description 53
- 239000002504 physiological saline solution Substances 0.000 claims abstract description 12
- 239000012530 fluid Substances 0.000 claims abstract description 10
- 238000010790 dilution Methods 0.000 claims abstract description 7
- 239000012895 dilution Substances 0.000 claims abstract description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 3
- 239000000725 suspension Substances 0.000 claims abstract 2
- 239000000243 solution Substances 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 241000186660 Lactobacillus Species 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 6
- 229940039696 lactobacillus Drugs 0.000 claims description 6
- 230000004913 activation Effects 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 8
- 238000005516 engineering process Methods 0.000 abstract description 3
- 235000012149 noodles Nutrition 0.000 abstract description 3
- 235000013312 flour Nutrition 0.000 abstract description 2
- 230000005526 G1 to G0 transition Effects 0.000 abstract 1
- 239000006872 mrs medium Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 7
- 230000002269 spontaneous effect Effects 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 230000035479 physiological effects, processes and functions Effects 0.000 description 5
- 239000006041 probiotic Substances 0.000 description 5
- 235000018291 probiotics Nutrition 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 238000004090 dissolution Methods 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 239000002956 ash Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 238000005065 mining Methods 0.000 description 2
- 229940100486 rice starch Drugs 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 235000002918 Fraxinus excelsior Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000186840 Lactobacillus fermentum Species 0.000 description 1
- 241000186869 Lactobacillus salivarius Species 0.000 description 1
- 208000005374 Poisoning Diseases 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 241000194020 Streptococcus thermophilus Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 208000005882 cadmium poisoning Diseases 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000003967 crop rotation Methods 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003631 expected effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cereal-Derived Products (AREA)
Abstract
The invention discloses a kind of methods of heavy metal cadmium in fermentation removing rice using lactobacillus plantarum, belong to rice deep process technology field.This method is the fermentation process for carrying out being inoculated with selected bacterium to the exceeded rice of cadmium, MRS medium cultures lactobacillus plantarum CGMCC No.6077 about 18h are centrifuged to stationary phase and are obtained bacterium mud, normal saline dilution, it is accessed with cell concentration 10^7cfu/mL and waits for fermentation system, rice in fermentation system：Physiological saline：Bacteria suspension is 40:60:1, fermentation condition is 37 DEG C, for 24 hours.Discard zymotic fluid after fermentation and wash rice, after rice is dried.After this treatment, the cadmium removal rate in rice reaches 80% or more, and gained rice can be used for producing rice flour or rice noodles product.Scientific achievement is applied to reality by the present invention well, is effectively reduced rice cadmium content, is solved the problems, such as cadmium pollution rice.
Description
Technical field
The present invention relates to a kind of methods of heavy metal cadmium in fermentation removing rice using lactobacillus plantarum, belong to rice and add deeply
Work technical field.
Background technology
Rice is one of staple food of the people of the world, and China is that big country is produced and consumed to rice, and have about 60% people with
Rice is staple food, and the edible safety of rice has some idea of.A large amount of investigation display, it is main that heavy metal pollution is that rice faces
One of pollution problem, and the most serious with cadmium pollution, the rice that many mining areas in China produce, seed cadmium content 100% are more than
0.2mg/kg (national limit standard), also shows the investigation of the commercially available rice in each department, and the common commercially available rice in area has 10%
Left and right is exceeded, and the commercially available rice exceeding standard rate of contaminated areas is more than 60%.Environmental Cadmium Pollution can not only influence the yield of rice, lead to grain
The shortage of food can also cause cadmium content in Rice Seed exceeded, the discarded meeting of a large amount of cadmium pollution rice because of the enrichment of plant
Cause huge economic loss；Meanwhile cadmium enters human body by approach such as diet, breathing and contacts, cadmium poisoning can seriously endanger
Health.
For the control of cadmium pollution, usually from source, control is set about at present, is mainly reflected in rehabilitating soil and water body, such as logical
Cross using special fertilizer or microorganism formulation control pollution, repair with contaminated soil, cadmium enriching plant and crops crop rotation,
Control mining area waste liquid and fertilizer all have modes, these modes such as pollution, the low cadmium accumulation ability rice varieties of screening in arable land
Certain effect, but its period is long and spends a large amount of manpower, material resources and financial resources, needs the support energetically of state's financial capacity and policy
An and long-term governance process.To having polluted the processing of rice, existing method has very much, is such as carried out at immersion to rice
Finishing processing, extraction rice starch, other rice foods such as alcohol is made etc. in reason, these processing modes can reduce rice or
Cadmium content in rice food, exists simultaneously some defects, such as period length, poor removal effect, low to rice utilization rate.
Studies have shown that probiotic lactobacillus CGMCC No.6077 have stronger cadmium tolerance and cadmium suction-operated, they
The Cd of 1000mg/kg concentration2+It can survive in solution, cadmium tolerable concentration reaches 10 times of other bacterial strains or more, and a concentration of
The Cd of 100mg/kg2+Cadmium adsorption rate is more than 20% in solution.The probiotics makes an addition in drinking-water or soya-bean milk, is poisoned to heavy metal cadmium
The poisoning symptom of mouse has mitigation, while the addition of the probiotics, can effectively reduce the cadmium content of fruit-vegetable juice beverage and carry
Its high inoxidizability, these researchs all apply to control cadmium pollution rice for probiotics and have laid a good foundation.
Currently, some patents and patent applications documents show fermentation process can along with the decline of cadmium content in rice,
Such as CN104585563A discloses a kind of method removing heavy metal cadmium in rice using compounding lactobacillus-fermented,
CN104489489A discloses a kind of method using heavy metal cadmium in lactobacillus and saccharomycete mixed fermentation abatement rice, just sends out
For ferment substrate, both the above method is all made of rice flour and carries out fermentation process, and centrifugation after fermentation obtains fermentation rice-flour noodles, this is specially
Profit is fermented relative to this using whole rice, is not only simplified zymotechnique, is decreased the waste of material in last handling process.
It is a kind of disclosed in CN104982807A to cut down the method also use whole rice fermentation of heavy metal cadmium in rice using zymotechnique, but do not have
Have and fermenting microbe is selected and is improved, add various lactic acid bacterias and zymotic fluid, pursues cadmium removal rate simply and have ignored hair
The definition of ferment system and safety.Meanwhile during mixed fungus fermentation, the control of bacterium solution proportioning is particularly important, only suitable
Proportioning under, could effectively play the effect of crucial bacterium, the unstable of bacterium solution proportioning may cause the unstable of technique, even
Expected effect is not achieved.
Invention content
The method that this patent is taken is realized is fermented using whole rice, is cleaned simplifying zymotechnique and reducing
In journey on the basis of the loss of rice starch, reach and the comparable even higher cadmium removal effect of existing patent.Meanwhile it is real
Current single bacterium kind is fermented, and cost is reduced, and is simplified technique, is avoided and unknown influence ingredient such as nutrition is added in fermentation system
Ingredient or zymotic fluid etc., so that it is determined that the mode of action of the bacterial strain in cadmium subtractive process.
The purpose of the present invention is to provide a kind of method simple for process, inexpensive, can be used for large-scale industrial production, profits
With a kind of probiotics, fermentation process is carried out to rice, can fast and effeciently remove the heavy metal cadmium in cadmium pollution rice, is realized
Pollute the recycling of rice.
The first purpose of the invention is to provide a kind of methods of removing rice heavy metal cadmium, and the method is to utilize plant
Heavy metal cadmium in lactobacillus CGMCC No.6077 fermentation removing rice.
In one embodiment of the invention, the lactobacillus plantarum CGMCC No.6077 are according to final concentration 10^7-10
The inoculum concentration of ^8cfu/mL is added in rice in steep system, and ferment 20~30h in 35~38 DEG C.
In one embodiment of the invention, rice in steep system is after eluriating the exceeded rice of cadmium, with physiology
Brine is uniformly mixed so as to obtain rice in steep system.
In one embodiment of the invention, the method is specially：
(1) the exceeded rice of cadmium is pressed into solid-liquid ratio 1:2~3 (g/mL) are eluriated 2~4 times with water；
(2) rice for eluriating water will be drained with physiological saline according to 1:The ratio mixing of 1~2 (g/mL) obtains rice leaching
Foam system；
(3) by lactobacillus plantarum CGMCC No.6077 bacterium mud normal saline dilutions, according to rice：Physiological saline：Bacterium is outstanding
Liquid is 30~60:40~80:1 (g/mL/mL) accesses above-mentioned rice in steep system, in 35~38 DEG C of constant temperature standing for fermentation 20~
30h removes zymotic fluid, obtains fermentation rice.
In one embodiment of the invention, the lactobacillus plantarum CGMCC No.6077 bacterium muds are by plant breast bar
Bacterium CGMCC No.6077 plate streakings activate, and access in MRS culture mediums in 35~38 DEG C of 15~20h of constant temperature incubation, centrifuge bacterium
Liquid, bacterium mud is resuspended with physiological saline and is cleaned, then is centrifuged, and bacterium mud is obtained.
In one embodiment of the invention, the method further includes eluriating fermentation rice 2~4 times using water, by institute
Rice drying is obtained, final rice moisture is 10-12%, obtains cadmium removing rice.
Second object of the present invention is to provide the cadmium removing rice that the method obtains.
Beneficial effects of the present invention：The present invention is using lactobacillus plantarum Production of lactic acid during the fermentation and to cadmium
Strong suction-operated, removing cadmium content are the heavy metal cadmium in the rice of 0.45mg/kg, and rice cadmium removal efficiency reaches 85% or more,
The cadmium of acquisition removes rice, can be used for being processed into rice meal or rice noodles.Simple for process, the cadmium removal efficiency height of this method,
Loss of proteins is small, and process costs are low, is suitable for large-scale industrial production, has a vast market application prospect and extensive society
It can economic benefit.
Description of the drawings
Fig. 1 is that fermentation system pH changes with time situation；
Fig. 2 is the cadmium concentration situation of change of zymotic fluid in fermentation process；
Fig. 3 is that the removal rate of cadmium under the conditions of different fermentations compares；
Fig. 4 is that the different removal rates for connecing the cadmium under bacterium amount compare；
Fig. 5 is that the cadmium removal rate under different strain sterile fermentation compares；
Fig. 6 is the protein content variation of the rice of different modes processing.
Specific implementation mode
In order to be more clearly understood that the technology contents of the present invention, spy are lifted following embodiment and are described in detail, purpose is only
It is to be best understood from the protection domain that present disclosure is not intended to limit the present invention.
Embodiment 1
By solid-liquid ratio 1：25g rice (cadmium content 0.45mg/kg) and 37.5mL physiology salts is added in conical flask in 1.5 (g/mL)
Water；5mLMRS accesses 1-2% bacterium solutions, and 37 DEG C of incubation 16-18h obtain activation bacterium solution；Per 10mL bacterium solutions in 4 DEG C of 5000g/min
10min is centrifuged, supernatant is abandoned, 10mL physiological saline is added and is resuspended, carries out gradient dilution as needed, obtains experiment bacterium solution；It is real
Group access bacterium solution is tested to a concentration of 10^7cfu/mL of liquid bacteria, 37 DEG C of standing for fermentation are for 24 hours.Respectively fermentation 4,8,12,16,
22, the pH value of fermentation system is measured for 24 hours.Known to result (Fig. 1), the pH of zymotic fluid is integrally on a declining curve, is reached in 16h or so
Stablize after to minimum, relative to spontaneous fermentation group, the pH of fermentation group (CGMCC No.6077 groups) reduces apparent, final fermentation
System pH is stablized 3.6 or so.
Embodiment 2
By solid-liquid ratio 1：25g rice (cadmium content 0.45mg/kg) and 37.5mL physiology salts is added in conical flask in 1.5 (g/mL)
Water；5mLMRS accesses 1-2% bacterium solutions, and 37 DEG C of incubation 16-18h obtain activation bacterium solution；Per 10mL bacterium solutions in 4 DEG C of 5000g/min
10min is centrifuged, supernatant is abandoned, 10mL physiological saline is added and is resuspended, carries out gradient dilution as needed, obtains experiment bacterium solution；It is real
Group access bacterium solution is tested to a concentration of 10^7cfu/mL of liquid bacteria, 37 DEG C of standing for fermentation are for 24 hours.One time fermentation supernatant is collected every 4h
(about 4mL), and detect the cadmium content in supernatant.As seen from Figure 2, the cadmium concentration of spontaneous fermentation group zymotic fluid is in relatively low
Level, and do not change substantially；Inoculum fermented group of zymotic fluid cadmium content significantly increases in 0-12h presentations, rear slow
Increase, the held stationary after the time reaches 20h；This result shows that, be implanted into the access of lactobacillus, played in fermentation system
Effect promotes the dissolution of cadmium in rice.In conjunction with the embodiments 1 pH variation tendencies it is found that the acid producing ability of bacterial strain to cadmium in rice
Dissolution play key effect.
Embodiment 3：
By solid-liquid ratio 1：25g rice (cadmium content 0.54mg/kg) and 37.5mL physiology salts is added in conical flask in 1.5 (g/mL)
Water；5mLMRS accesses 1-2% bacterium solutions, and 37 DEG C of incubation 16-18h obtain activation bacterium solution；Per 10mL bacterium solutions in 4 DEG C of 5000g/min
10min is centrifuged, supernatant is abandoned, 10mL physiological saline is added and is resuspended, carries out gradient dilution as needed, obtains experiment bacterium solution；It is real
Group access bacterium solution is tested to a concentration of 10^7cfu/mL of liquid bacteria, 37 DEG C of standing for fermentation for 24 hours, are cleaned greatly with the ultra-pure water of 2 times of volumes
Rice 3 times, 60 DEG C of drying overnight obtain cadmium removing rice.After measured, the cadmium content of cadmium removing rice is 0.078mg/kg, is less than
National limit standard 0.2mg/kg, cadmium removal efficiency reach 82.67%.
Reference examples 1：
Lactobacillus plantarum is not accessed in rice in steep liquid, other steps are implemented according to case 1, only rely on spontaneous fermentation, greatly
Under the action of soaking of meter Shui and spontaneous fermentation effect, as a result see that Fig. 3, cadmium removal efficiency are only 25.80%.
Reference examples 2：
Rice in steep fermentation system does not access lactobacillus plantarum, and fermentation temperature is changed to 4 DEG C of control spontaneous fermentations, other
Step is implemented according to case 1, as a result sees that Fig. 3, cadmium removal efficiency are only 16.6%.
Reference examples 3：
Rice in steep fermentation system accesses the dead thalline of lactobacillus plantarum, other steps are implemented according to case 1, as a result see Fig. 3,
Cadmium removal efficiency is 61.3%.
Reference examples 4：
Rice in steep fermentation system accesses lactobacillus plantarum, a concentration of 10^1-10^8cfu/mL of liquid bacteria, other steps are pressed
Implement according to case 1, as a result sees that Fig. 4, cadmium removal efficiency are 19.6%-82.67%.
Embodiment 4：
By solid-liquid ratio 1：25g rice (cadmium content 0.62mg/kg) and 37.5mL physiology salts is added in conical flask in 1.5 (g/mL)
Water；5mLMRS accesses 1-2% bacterium solutions, and 37 DEG C of incubation 16-18h obtain activation bacterium solution；Per 10mL bacterium solutions in 5000g/min4 DEG C from
Heart 10min abandons supernatant, and 10mL physiological saline is added and is resuspended, carries out gradient dilution as needed, obtains experiment bacterium solution；Experiment
Group access bacterium solution to a concentration of 10^7cfu/mL of liquid bacteria, 37 DEG C of standing for fermentation for 24 hours, rice 3 are cleaned with the ultra-pure water of 2 times of volumes
Secondary, 60 DEG C of drying overnight obtain cadmium removing rice.After measured, the cadmium content of cadmium removing rice is 0.064mg/kg, less than country
Limit standard 0.2mg/kg, cadmium removal efficiency reach 89.68%.
Reference examples 5：
For research lactobacillus plantarum CGMCC No.6077 as single fermentation agent removal rice in cadmium ability, respectively with
Other common rice fermentation agent strain (lactobacillus plantarum CCFM595, lactobacillus fermenti LF3, Lactobacillus salivarius LS11, Luo Yishi
Lactobacillus LR90, streptococcus thermophilus ST07, bulgarian milk bar LB2) it is compareed.Rice in steep fermentation system is respectively connected to
The above bacterial strain, bacterium solution cell concentration are 10^7cfu/mL, other steps are implemented according to case 4, as a result see Fig. 5, cadmium removal efficiency according to
Secondary is 45.7%, 32.2%, 75.8%, 62%, 28.5% and 37.7%.On sterile fermentation, lactobacillus plantarum CGMCC
There is absolute predominances for cadmium removal in rice by No.6077, while there is also larger differences for lactobacillus plantarum inter-species, it is seen that
Strain fermentation removes cadmium in the presence of specificity.
Embodiment 5：
By in embodiment 4 lactobacillus plantarum CGMCC No.6077 and the fermentation process of other existing strains compare
Compared with.Have that part takes in existing zymotechnique is leavening or zymotic fluid as strain source, does not embody specific zymophyte
Kind, the uncertainty for causing fermentation system to form, it cannot be guaranteed that being perfectly safe；Some take more bacterium combined ferments and connect bacterium amount compared with
Greatly, it ferments relative to the single bacterium of this patent, technique is relative complex, and cost is relatively higher；What is more, additional in fermentation system
Some substances are added, the edible safety of tunning is cannot be guaranteed while increasing cost.
Table 1：The present invention and the strain advantage of existing research or patent compare
Table 2：The present invention is compared from the different extra process modes of existing research or patent
Embodiment 6：
Ingredient before and after rice fermentation is measured and is compared, the index mainly measured has albumen, crude fat and ash content
Content, in terms of butt.3, after fermentation are the results are shown in Table, rice protein content and content of ashes significantly reduce, and fat content, which also has, to be subtracted
It is few；Wherein there is also bigger differences between spontaneous fermentation and bacterium fermentation.The reduction of protein content mainly has both sides reason,
On the one hand, the variation of rice structure contributes to the dissolution of albumen, on the other hand, the generation of the fermentation and acid of bacterium, albumen
It is decomposed into smaller albumen, peptide even amino acid, these are all the reason of causing protein content to reduce；Further research is aobvious
Show, the reduction of albumen is mainly reflected in the significant decrease (Fig. 6) of glutelin content.The reduction of ash content, a part is by water-soluble
Free state ion provides, and a part is reduced by reference state ion along with the destruction or dissolution of the conjugates such as albumen.
Table 3：The front and back rice main component variation of fermentation
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not limited to the present invention, any to be familiar with this skill
The people of art can do various change and modification, therefore the protection model of the present invention without departing from the spirit and scope of the present invention
Enclosing be subject to what claims were defined.
Claims (8)
1. a kind of method of removing rice heavy metal cadmium, which is characterized in that the method is to utilize lactobacillus plantarum CGMCC
Heavy metal cadmium in No.6077 fermentation removing rice.
2. method according to claim 1, which is characterized in that the lactobacillus plantarum CGMCC No.6077 are according to final concentration
The inoculum concentration of 10^7-10^8cfu/mL is added in rice in steep system.
3. method according to claim 2, which is characterized in that rice in steep system is after eluriating the exceeded rice of cadmium,
It is uniformly mixed so as to obtain rice in steep system with physiological saline.
4. method according to claim 1, which is characterized in that the fermentation is 20~30h of fermentation at 35~38 DEG C.
5. method according to claim 1, which is characterized in that the method is specially：
(1) the exceeded rice of cadmium is pressed into solid-liquid ratio 1:2~3 (g/mL) are eluriated 2~4 times with water；
(2) rice for eluriating water will be drained with physiological saline according to 1:The ratio mixing of 1~2 (g/mL), obtains rice in steep body
System；
(3) by lactobacillus plantarum CGMCC No.6077 bacterium mud normal saline dilutions, according to rice：Physiological saline：Bacteria suspension is
30~60:40~80:1 (g/mL/mL) accesses above-mentioned rice in steep system, is left to ferment 20~30h in 35~38 DEG C of constant temperature, goes
Except zymotic fluid, fermentation rice is obtained.
6. method according to claim 5, which is characterized in that the lactobacillus plantarum CGMCC No.6077 bacterium muds are by planting
Object lactobacillus CGMCC No.6077 activation accesses in MRS culture mediums in 35~38 DEG C of 15~20h of constant temperature incubation, centrifuges bacterium solution,
Bacterium mud is resuspended with physiological saline and is cleaned, then is centrifuged, and bacterium mud is obtained.
7. method according to claim 5, which is characterized in that the method further includes eluriating fermentation rice 2~4 using water
It is secondary, gained rice is dried, final rice moisture is 10-12%, obtains cadmium removing rice.
8. the cadmium that any the method for claim 1~7 obtains removes rice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810033884.9A CN108294224A (en) | 2018-01-15 | 2018-01-15 | A method of it is fermented using lactobacillus plantarum and removes heavy metal cadmium in rice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810033884.9A CN108294224A (en) | 2018-01-15 | 2018-01-15 | A method of it is fermented using lactobacillus plantarum and removes heavy metal cadmium in rice |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108294224A true CN108294224A (en) | 2018-07-20 |
Family
ID=62868968
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810033884.9A Pending CN108294224A (en) | 2018-01-15 | 2018-01-15 | A method of it is fermented using lactobacillus plantarum and removes heavy metal cadmium in rice |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108294224A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110226705A (en) * | 2019-07-24 | 2019-09-13 | 湖南粮食集团有限责任公司 | A method of strengthening cadmium in spontaneous fermentation abatement rice containing cadmium |
| CN111109508A (en) * | 2018-10-31 | 2020-05-08 | 中南粮油食品科学研究院有限公司 | Method for reducing cadmium in rice by using compound biological enzyme and compound lactic acid bacteria |
| CN111197012A (en) * | 2018-11-17 | 2020-05-26 | 原生生物医学股份有限公司 | Novel lactobacillus plantarum, lactobacillus composition and use thereof for treating or preventing heavy metal-related diseases |
| CN112167305A (en) * | 2020-09-27 | 2021-01-05 | 浙江青莲食品股份有限公司 | Cleaning method for removing peculiar smell of pig large intestine |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102827796A (en) * | 2012-09-03 | 2012-12-19 | 江南大学 | Lactobacillus plantarum with cadmium removing function and usage thereof |
| CN104489489A (en) * | 2015-01-12 | 2015-04-08 | 湖南农业大学 | Method for reducing heavy metal cadmium in rice by utilizing mixed fermentation of lactobacillus and saccharomycetes |
| CN104982807A (en) * | 2015-06-15 | 2015-10-21 | 金健米业股份有限公司 | Method for reducing heavy metal cadmium in rice through fermentation technology |
| CN105325825A (en) * | 2015-11-24 | 2016-02-17 | 江南大学 | Fruit and vegetable juice improving method capable of improving oxidation resistance and removing heavy metal cadmium simultaneously |
-
2018
- 2018-01-15 CN CN201810033884.9A patent/CN108294224A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102827796A (en) * | 2012-09-03 | 2012-12-19 | 江南大学 | Lactobacillus plantarum with cadmium removing function and usage thereof |
| CN104489489A (en) * | 2015-01-12 | 2015-04-08 | 湖南农业大学 | Method for reducing heavy metal cadmium in rice by utilizing mixed fermentation of lactobacillus and saccharomycetes |
| CN104982807A (en) * | 2015-06-15 | 2015-10-21 | 金健米业股份有限公司 | Method for reducing heavy metal cadmium in rice through fermentation technology |
| CN105325825A (en) * | 2015-11-24 | 2016-02-17 | 江南大学 | Fruit and vegetable juice improving method capable of improving oxidation resistance and removing heavy metal cadmium simultaneously |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111109508A (en) * | 2018-10-31 | 2020-05-08 | 中南粮油食品科学研究院有限公司 | Method for reducing cadmium in rice by using compound biological enzyme and compound lactic acid bacteria |
| CN111197012A (en) * | 2018-11-17 | 2020-05-26 | 原生生物医学股份有限公司 | Novel lactobacillus plantarum, lactobacillus composition and use thereof for treating or preventing heavy metal-related diseases |
| CN110226705A (en) * | 2019-07-24 | 2019-09-13 | 湖南粮食集团有限责任公司 | A method of strengthening cadmium in spontaneous fermentation abatement rice containing cadmium |
| CN112167305A (en) * | 2020-09-27 | 2021-01-05 | 浙江青莲食品股份有限公司 | Cleaning method for removing peculiar smell of pig large intestine |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108294224A (en) | A method of it is fermented using lactobacillus plantarum and removes heavy metal cadmium in rice | |
| CN104388514B (en) | The method that gamma aminobutyric acid is prepared using composite bacteria fermentation | |
| CN103232963B (en) | Collagenase producing strain | |
| CN104323222B (en) | A kind of bacterium mushroom powder being rich in farnoquinone and preparation method thereof | |
| CN114456979B (en) | Intestinal membrane-like Weissella for promoting production of flavor substances in fermented food and application thereof | |
| CN104762238A (en) | Lactic acid bacteria not generating amino acid decarboxylase high-yield urease and application of lactic acid bacteria | |
| CN113430147B (en) | Bacillus villagens QH-20011 with low pH tolerance and application thereof | |
| CN114540231B (en) | Pediococcus acidilactici for promoting production of flavor substances in fermented food and application thereof | |
| CN105062677A (en) | Method for preparing fermented beet extract through processing beet juice by Lactobacillus plantarum | |
| CN105441343A (en) | Saccharomyces cerevisiae and Saccharomyces cerevisiae culture thereof | |
| CN113498834A (en) | Integrated fermentation method of edible and medicinal fungus fermented beverage | |
| CN104342372B (en) | Method for producing yeast autolysate by probiotic fermentation | |
| CN103289925A (en) | Lactic acid bacteria strain for high yield of amylase and application thereof | |
| CN103146769A (en) | Method for preparating citric acid by fermentation | |
| CN106035985A (en) | Method for producing single cell proteins by using processed waste from mixed bacteria liquid fermentation of yellow wine | |
| CN109700014A (en) | A kind of preparation method of rice bran probiotics ferment | |
| CN107118885B (en) | Method for producing fermented wine containing GABA (Gamma amino acid butyric acid) by using ethanol-resistant pediococcus | |
| CN106635834B (en) | The Thelephora ganbajun mycelium zinc polysaccharide and application that one plant of wizened bacteria strain and its fermentation obtain | |
| CN115595332A (en) | Method for preparing tremella fermentation liquor by combining compound enzyme with microbial fermentation | |
| CN112522114B (en) | Cordyceps militaris fungus chaff extract, ganoderma lucidum fermentation product, and preparation methods and applications thereof | |
| CN102433289B (en) | Strain for producing citrulline and method for biologically synthesizing citrulline with same | |
| CN109401998B (en) | Lactobacillus mindendori for degrading biogenic amine and application thereof | |
| CN107937318B (en) | Bacillus subtilis MXT-1 for degrading wheat pentosan and application thereof | |
| CN103865865B (en) | A kind of sea cucumber enteron aisle produces Sumizyme MP bacterial strain and application thereof | |
| CN110150505A (en) | A kind of lactic acid bacteria complexing agent and preparation method thereof removing Eriocheir sinensis bilgy odour |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180720 |
|
| RJ01 | Rejection of invention patent application after publication |