CN108279491B - Long working distance and high resolution video microscope for biological observation - Google Patents
Long working distance and high resolution video microscope for biological observation Download PDFInfo
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- CN108279491B CN108279491B CN201810357221.2A CN201810357221A CN108279491B CN 108279491 B CN108279491 B CN 108279491B CN 201810357221 A CN201810357221 A CN 201810357221A CN 108279491 B CN108279491 B CN 108279491B
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- 239000011521 glass Substances 0.000 claims description 10
- 238000002310 reflectometry Methods 0.000 claims description 10
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- 230000003287 optical effect Effects 0.000 claims description 7
- 238000004026 adhesive bonding Methods 0.000 claims description 3
- 238000005452 bending Methods 0.000 claims description 3
- 230000005499 meniscus Effects 0.000 claims description 3
- 238000002834 transmittance Methods 0.000 claims description 3
- 230000004075 alteration Effects 0.000 abstract description 22
- 238000004113 cell culture Methods 0.000 abstract description 3
- 235000017284 Pometia pinnata Nutrition 0.000 abstract description 2
- 240000007653 Pometia tomentosa Species 0.000 abstract description 2
- 238000003384 imaging method Methods 0.000 description 10
- 238000013461 design Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 201000009310 astigmatism Diseases 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 229960000074 biopharmaceutical Drugs 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 101000713585 Homo sapiens Tubulin beta-4A chain Proteins 0.000 description 1
- 206010073261 Ovarian theca cell tumour Diseases 0.000 description 1
- 102100036788 Tubulin beta-4A chain Human genes 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000005331 crown glasses (windows) Substances 0.000 description 1
- 239000005308 flint glass Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 208000001644 thecoma Diseases 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
- G02B21/04—Objectives involving mirrors
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Abstract
The invention provides a long working distance and high resolution video microscope for biological observation, which comprises a List objective lens group consisting of a front group of cemented lenses and a rear group of cemented lenses, a secondary mirror and a main mirror group Cheng Kasai Grine mirror system, wherein an aperture diaphragm is arranged in front of the List objective lens group, and an imaging-free part generated by shielding of the secondary mirror is eliminated; the Cassegrain reflector system is used in a video microscope, and due to the existence of the main reflector, the caliber of the Cassegrain reflector system can be large enough to receive as much energy as possible to ensure illuminance, so that the numerical aperture is improved, the resolution is improved, and meanwhile, the Cassegrain reflector system has no chromatic aberration, so that the aberration is eliminated, the object space resolution is ensured, the high resolution is ensured while the long working distance is ensured, and the cell culture can be clearly observed; the video microscope provided by the invention works in the wave band of 400-700nm, the working distance can reach 100mm, the numerical aperture is 0.38mm, and the object space sight field is 1.2mm.
Description
Technical Field
The invention belongs to the field of biomedical engineering, and particularly relates to a long-working-distance high-resolution video microscope for biological observation.
Background
Video microscopes are currently widely used in the industrial fields of machine vision, reading measurement, etc., and are rarely used in the biomedical field due to the limitations of resolution and working distance. At present, biological laboratories of pharmaceutical enterprises and scientific research institutions all use traditional inverted microscopes to observe cells, and the inverted microscopes have high resolution, but have small working distances, and the working distances are only 0.1-3mm. In summary, the existing inverted microscope on the market has high resolution, but short working distance, long working distance and low resolution; the thickness of the culture bottle body is 30mm, the thickness of each layer of the cell factory is 20mm, the inclined observation working distance is more than 80mm, and the heights of the cell fermentation tanks with different specifications are different from 18mm to 100mm, so that the inverted microscope and the industrial video microscope on the market cannot be directly used for observing cells in culture dishes such as the culture bottle, the cell factory and the cell fermentation tank.
Disclosure of Invention
In order to solve the problem that the traditional inverted microscope cannot be directly used for observing cells in culture bottles, cell factories and cell fermentation tanks in the biopharmaceutical field in the prior art, the invention aims to provide a novel microscope objective with long working distance and high resolution combining a List type objective lens and a Cassegrain Lin Fanshe lens, so that the working distance and resolution of a video microscope are improved, the observation in the biopharmaceutical field is facilitated, the quality monitoring of pharmaceutical enterprises is ensured, the artificial pollution is avoided, and the production efficiency of the enterprises is comprehensively improved.
The invention provides a long working distance and high resolution video microscope for biological observation, which sequentially comprises the following components from front to back along the same optical axis direction: the lens comprises an aperture diaphragm, a front group of cemented lenses, a rear group of cemented lenses, a flat plate, a secondary reflector and a main reflector, wherein the secondary reflector is fixedly connected with the flat plate, the main reflector is a plano-concave spherical lens, a through hole is formed in the center of the plano-concave spherical lens, and the concave surface of the plano-concave spherical lens is close to the secondary reflector.
According to the invention, the Cassegrain reflecting system is introduced into the video microscope, and the caliber of the main reflecting mirror in the Cassegrain reflecting system can be made large enough, so that more energy can be received, and the illuminance is ensured. The increase of the caliber can cause marginal aberration and the imaging is unclear, but the invention adopts a mode of combining a Cassegrain reflecting system and a List type objective lens group, reduces the number of optical elements, eliminates spherical aberration and corrects aberration.
The invention provides a video microscope, which comprises a List objective lens group formed by a front group of cemented lenses and a rear group of cemented lenses, and a Cassegrain mirror system formed by a secondary mirror and an annular main mirror.
Further, the center of the surface of the aperture diaphragm is plated with a circular reflection film, the edge of the surface is plated with an annular anti-reflection film, the reflectivity of the reflection film is more than 94%, and the transmittance of the anti-reflection film is more than 98.6%. The video microscope provided by the invention is provided with the circular high-reflectivity film plated at the center of the aperture diaphragm, the periphery of the reflection film, namely the edge of the surface of the aperture diaphragm is plated with the annular anti-reflection film, so that the edge light of the system is further limited, and the spherical aberration is corrected.
Further, the front group cemented lens comprises a first lens and a second lens, the first lens is a positive focal power lens, the second lens is a negative focal power lens, the rear group cemented lens comprises a third lens and a fourth lens, the third lens is a negative focal power lens, and the fourth lens is a positive focal power lens.
Further, the first lens, the second lens, the third lens and the fourth lens are all meniscus lenses, the concave surface of the first lens is close to the aperture stop, the bending directions of the first lens, the second lens, the third lens and the fourth lens are consistent, the convex surface of the first lens is attached to the concave surface of the second lens, and the convex surface of the third lens is attached to the concave surface of the fourth lens.
Further, a field stop is arranged between the second lens and the third lens.
The long working distance and high resolution video microscope comprises 4 lenses, namely a first lens, a second lens, a third lens and a fourth lens in sequence, wherein the first lens is arranged in sequence from the object plane: a positive power lens; a second lens: a negative power lens; and a third lens: a negative power lens; fourth lens: a positive power lens. The first lens and the second lens are one group, the third lens and the fourth lens are the other group, the two groups of cemented lenses are similar to a double Gaussian structure, a field stop is added between the lens groups to limit the height of incident light rays, the convex surfaces of the second lens and the third lens are bent to the field stop, the astigmatism and the field area can be corrected, the first lens, the second lens, the third lens and the fourth lens are all positive-negative focal power lenses, and the cementing can correct spherical aberration. The two groups of lenses are in a symmetrical double Gaussian structure, which is favorable for dispersing focal power burden and correcting off-axis point advanced aberration.
Further, the abbe number of the first lens is 70.13 or more, the abbe number of the third lens is 64.11 or more, the refractive index of the second lens is 1.80 or more, and the refractive index of the fourth lens is 1.78 or more. The Abbe number is the reciprocal of the dispersion coefficient, the first lens and the third lens of the invention are crown glass with small dispersion coefficient, the second lens and the fourth lens are flint glass with high reflectivity, and the design has clear imaging and high resolution.
Further, the secondary reflector is a plano-convex lens, the plane of the plano-convex lens is glued with the flat plate, the convex surface is close to the main reflector, a reflecting film is plated on the plano-convex lens, the reflectivity of the reflecting film is more than 93%, and the reflecting film with high reflectivity further promotes the improvement of imaging definition.
The video microscope comprises a main reflector and a secondary reflector, wherein the secondary reflector is a plano-convex spherical lens glued with a flat plate to ensure coaxiality, the main reflector is a plano-concave spherical lens, a through hole is arranged in the center of the plano-concave spherical lens, and the concave surface of the plano-concave spherical lens is close to the secondary reflector. According to the invention, the secondary reflector is fixed on the flat plate in a gluing mode, the flat plate is made of optical material k9 glass, an extremely thin adhesive is coated on the k9 glass, and then the secondary reflector is fixed on the flat plate, so that light blocking of the flat plate is avoided, and imaging illuminance is ensured. Compared with the traditional tripod fixing mode, the invention has the advantages of high transmission rate of middle light and sufficient imaging illuminance.
Further, the diameter of the main reflector is 80-100mm, the diameter of the through hole in the center of the main reflector is 20-24mm, and further, the diameter of the secondary reflector is 30-38cm.
Further, the diameter of the aperture diaphragm is 38-42mm, and the diameter of the central round hole is 16-20mm.
The working wave band of the video microscope provided by the invention is 400-700nm, the working distance is within 100mm, the numerical aperture is 0.38mm, and the object space line view field is 1.2mm.
The beneficial effects of the invention are as follows:
the invention provides a video microscope, which comprises a List objective lens group consisting of a front group of cemented lenses and a rear group of cemented lenses, and a secondary mirror and a main mirror group Cheng Kasai Grignard mirror system, wherein an aperture diaphragm is arranged in front of the List objective lens group, so that the non-imaging part generated by shielding of the secondary mirror is eliminated, the microscope is designed by combining the List objective lens and the Grignard mirror system, the Grignard mirror system is used in the microscope, and due to the existence of the main mirror, the caliber of the main mirror can be large enough to receive as much energy as possible to ensure illuminance, improve the numerical aperture and the resolution, and meanwhile, the Grignard mirror system has no chromatic aberration, so that aberration can be eliminated, and the resolution of an object side is ensured, so that the long working distance is ensured, meanwhile, the microscope has high resolution, and cell culture can be clearly observed; the video microscope provided by the invention works in the wave band of 400-700nm, the working distance can reach 100mm, the numerical aperture NA is 0.38mm, and the object space sight field is 1.2mm.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view of a video microscope provided by the present invention;
FIG. 2 is a graph of the MTF transfer function of a video microscope provided by the present invention;
fig. 3 is a dot column diagram of a video microscope of the present invention.
1, an aperture diaphragm; 2. a front group of cemented lenses; 3. a rear group cemented lens; 4. a flat plate; 5. a secondary mirror; 6. a primary mirror; 7. a through hole; 8. a first lens; 9. a second lens; 10. a third lens; 11. a fourth lens; 12. a field stop; 13. an object plane.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, based on the examples herein, which are within the scope of the invention as defined by the claims, will be within the scope of the invention as defined by the claims.
The present invention provides a long working distance, high resolution video microscope for biological observation, the microscope comprising, from front to back in the same optical axis direction: the aperture diaphragm 1, the front group cemented lens 2, the back group cemented lens 3, the flat board 4, secondary mirror 5 and main mirror 6, secondary mirror 5 with flat board 4 fixed connection, main mirror 6 is plano-concave spherical lens, plano-concave spherical lens center is provided with through-hole 7, plano-concave spherical lens's concave surface is close to secondary mirror 5. In the video microscope provided by the invention, the List objective lens group is formed by the front group of the cemented lens 2 and the rear group of the cemented lens 3, the Cassegrain mirror system is formed by the secondary mirror 5 and the main mirror 6, the video microscope is designed by combining the List objective lens and the Cassegrain mirror system, the Cassegrain mirror system is used in the video microscope, and due to the existence of the main mirror 6, the caliber of the main mirror 6 can be large enough to accept as much energy as possible to ensure illuminance, improve the numerical aperture and improve the resolution, and meanwhile, the Cassegrain mirror system has no chromatic aberration, so that aberration can be eliminated, the object resolution is ensured, and the long working distance is ensured, meanwhile, the high resolution is provided, and the cell culture can be clearly observed. The video microscope provided by the invention has a working wave band of 400-700nm, a working distance of less than 100mm, a numerical aperture of 0.38mm and an object space line view field of 1.2mm.
As an alternative embodiment, the center of the surface of the aperture diaphragm 1 is plated with a circular reflection film, the edge of the surface is plated with an annular reflection reducing film, the reflectivity of the reflection reducing film is more than 94%, and the transmittance of the reflection reducing film is more than 98.6%. The invention provides a video microscope, which comprises a List objective lens group formed by a front group of cemented lenses 2 and a rear group of cemented lenses 3, and a Cassegrain mirror system formed by a secondary mirror 5 and an annular main mirror 6, wherein an aperture diaphragm 1 is arranged in front of the List objective lens group, a circular high-reflectivity film is plated at the center of the aperture diaphragm 1, and an annular antireflection film is plated at the periphery of the reflection film, namely, the edge of the surface of the aperture diaphragm 1.
As an alternative embodiment, the front group cemented lens 2 includes a first lens 8 and a second lens 9, the first lens 8 is a positive power lens, the second lens 9 is a negative power lens, the rear group cemented lens 3 includes a third lens 10 and a fourth lens 11, the third lens 10 is a negative power lens, and the fourth lens 11 is a positive power lens.
As an alternative embodiment, the first lens 8, the second lens 9, the third lens 10 and the fourth lens 11 are all meniscus lenses, the concave surface of the first lens 8 is close to the aperture stop 1, the bending directions of the first lens 8, the second lens 9, the third lens 10 and the fourth lens 11 are consistent, the convex surface of the first lens 8 is attached to the concave surface of the second lens 9, and the convex surface of the third lens 10 is attached to the concave surface of the fourth lens 11.
As an alternative embodiment, a field stop 12 is arranged between the second lens 9 and the third lens 10.
The long working distance and high resolution video microscope of the invention comprises 4 lenses, namely a first lens 8, a second lens 9, a third lens 10 and a fourth lens 11 in sequence, wherein the first lens 8 is arranged in sequence from the direction of an object plane 13: a positive power lens; the second lens 9: a negative power lens; third lens 10: a negative power lens; fourth lens 11: a positive power lens. The first lens 8 and the second lens 9 are one group, the third lens 10 and the fourth lens 11 are the other group, the two groups of cemented lenses are similar to a double-Gaussian structure, a field stop 12 is added between the lens groups to limit the height of incident light, the convex surfaces of the second lens 10 and the third lens 10 are bent towards the field stop 12, which is favorable for correcting astigmatism and a field area, and the first lens 8, the second lens 9, the third lens 10 and the fourth lens 11 are all positive-negative focal power lenses, so that spherical aberration can be corrected by cementing. The two groups of lenses are in a symmetrical double Gaussian structure, which is favorable for dispersing focal power burden and correcting off-axis point advanced aberration.
As an alternative embodiment, the plane of the plano-convex lens is glued to the flat plate 4, the convex surface is close to the main reflector 6, and a reflective film is plated on the plano-convex lens, the reflectivity of the reflective film is more than 93%, and the reflective film with high reflectivity further promotes the imaging definition.
The video microscope comprises a main reflector 6 and a secondary reflector 5, wherein the secondary reflector 5 is a plano-convex spherical lens glued with the flat plate 4, coaxiality is guaranteed, the main reflector 6 is a plano-concave spherical lens, a through hole 7 is formed in the center of the plano-concave spherical lens, and the concave surface of the plano-concave spherical lens is close to the secondary reflector 5. In the invention, the secondary reflector 5 is fixed on the flat plate 4 in a gluing mode, the flat plate 4 is made of optical material k9 glass, an extremely thin adhesive is coated on the k9 glass, and then the secondary reflector 5 is fixed on the flat plate 4, so that the light blocking of the flat plate 4 is avoided, and the imaging illuminance is ensured. Compared with the traditional tripod fixing mode, the invention has the advantages of high transmission rate of middle light and sufficient imaging illuminance.
As an alternative embodiment, the diameter of the main reflector 6 is 80-100mm, and the diameter of the through hole 7 in the center of the main reflector 6 is 20-24mm.
As an alternative embodiment, the diameter of the secondary mirror 5 is 30-38mm.
As an alternative embodiment, the diameter of the aperture stop 1 is 38-42mm, and the diameter of the central circular hole is 16-20mm.
The video microscope device of the invention comprises a main reflector 6 and a secondary reflector 5, wherein the secondary reflector is a plano-convex lens glued with a flat plate 4, coaxiality is ensured, the main reflector 6 is a plano-concave spherical lens, a concave surface is bent to an object plane 13, the caliber is 80-100mm, and the diameter of a round hole opened in the middle is 20-24mm.
Table 1 shows the parameters of the microscope. Table 2 shows the specific parameter values of each lens of the long working distance and high resolution video microscope for biological observation provided by the invention, wherein the column of the "radius" indicates the spherical radius corresponding to each surface, and the column of the "thickness/interval" indicates the thickness of the lens and the air interval (axial distance of the lens center) between the two lens groups. "Material" refers to the corresponding lens material, and "effective clear aperture" refers to the actual light height of the face. In the table, G1 represents the first lens 8, G2 represents the second lens 9, G3 represents the third lens 10, and G4 represents the fourth lens 11.
The front group cemented lens 2 includes G1 and G2, the rear group cemented lens 3 includes G3 and G4, and the front group cemented lens 2 is exemplified by a front surface T1 plane, a rear surface T2 plane, a spherical radius of T1 is-291.655, a spherical radius of T2 is-111.517, an axial distance from T1 to the object plane 13 is 100mm (working distance), an axial distance from T1 to T2 plane is G1 glass thickness 10mm, and a glass material is H-QK 3. The effective clear aperture of the T1 face is 40.5mm, and the effective clear aperture of the rear face T2 is 43.3mm.
Since G1 and G2 are the front group of cemented lenses 2, the spherical radius of the front surface T3 of G2 is-111.517, the spherical radius of the rear surface T4 is-149.41, the axial distance from T3 to T4 is 8.5mm of the glass thickness of G2, and the glass material is H-ZLAF 1. The effective clear aperture of the T3 face (t3=t2) is 43.3mm and the effective clear aperture of the rear face T4 is 46.7mm. The remaining parameters in Table 2 are based on G1, G2, and so on.
The spherical radius of the main reflector 6 and the secondary reflector is-141.6, -96.84, the main reflector 6 is a plano-concave spherical lens, the concave surface is bent towards the object plane 13, the secondary reflector is a plano-convex lens, and the convex surface is bent towards the main reflector. The glass materials are H-K9L, and the effective pore diameters are 87.8mm and 37.8mm respectively. The specific structural design is shown in figure 1.
Fig. 2 shows a graph of MTF transfer function for a video microscope of the present invention.
From the function curve of fig. 2, it is seen that at a cut-off frequency of 100lp/mm, the average transfer function reaches more than 0.16, and the meridian and the sagittal line coincide well, and are all close to the diffraction limit. The system is shown to have good imaging quality.
Fig. 3 shows a dot column diagram of a video microscope of the present invention.
As can be seen from the data on the graph, the root mean square speckle RMS is smaller than the Yu Aili speckle radius 8.952um, and the overall speckle GEO is smaller than the airy speckle radius, indicating that the system resolution is very high. The microscope belongs to a small aberration system, and when correcting aberration, the spherical aberration, the coma aberration and the position chromatic aberration are emphasized and three kinds of aberration are very small as can be seen from a point column diagram, and the three kinds of aberration are smaller than the aberration tolerance through calculation, so that the design result is reasonable.
TABLE 1
| Working distance (L) | 100mm |
| Numerical aperture NA | 0.38mm |
| Object space line field of view | 1.2mm |
| Operating band | 400-700nm |
| Barrel length | 625mm |
| Magnification beta | -5× |
TABLE 2
The foregoing is merely illustrative of the present invention, and the present invention is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (8)
1. A long working distance, high resolution video microscope for biological observation comprising, in order from front to back along the same optical axis: the lens comprises an aperture diaphragm, a front group of cemented lenses, a rear group of cemented lenses, a flat plate, a secondary reflector and a main reflector, wherein the secondary reflector is fixedly connected with the flat plate, the main reflector is a plano-concave spherical lens, a through hole is formed in the center of the plano-concave spherical lens, and the concave surface of the plano-concave spherical lens is close to the secondary reflector;
the front group of the cemented lens comprises a first lens and a second lens, the first lens is a positive focal power lens, the second lens is a negative focal power lens, the rear group of the cemented lens comprises a third lens and a fourth lens, the third lens is a negative focal power lens, and the fourth lens is a positive focal power lens;
the first lens, the second lens, the third lens and the fourth lens are all meniscus lenses, the concave surface of the first lens is close to the aperture diaphragm, the bending directions of the first lens, the second lens, the third lens and the fourth lens are consistent, the convex surface of the first lens is jointed with the concave surface of the second lens, and the convex surface of the third lens is jointed with the concave surface of the fourth lens;
the secondary reflector is fixed on a flat plate in a gluing mode, and the flat plate is made of optical material k9 glass.
2. The long working distance, high resolution video microscope for biological observation according to claim 1, wherein the center of the aperture stop surface is plated with a circular reflection film, the edge of the surface is plated with an annular antireflection film, the reflectance of the reflection film is 94% or more, and the transmittance of the antireflection film is 98.6% or more.
3. The long working distance, high resolution video microscope for biological observation according to claim 1, wherein a field stop is provided intermediate the second and third lenses.
4. A long working distance, high resolution video microscope for biological observation according to claim 3 wherein the abbe number of the first lens is 70.13 or more, the abbe number of the third lens is 64.11 or more, the refractive index of the second lens is 1.80 or more, and the refractive index of the fourth lens is 1.78 or more.
5. The long working distance, high resolution video microscope for biological observation according to claim 4, wherein the secondary mirror is a plano-convex lens, the plane of the plano-convex lens is glued to the flat plate, the convex surface is close to the primary mirror, the plano-convex lens is coated with a reflective film, and the reflectivity of the reflective film is 93% or more.
6. The long working distance, high resolution video microscope for biological observation according to claim 5, wherein the diameter of the main mirror is 80-100mm, and the diameter of the through hole in the center of the main mirror is 20-24mm.
7. The long working distance, high resolution video microscope for biological observation according to claim 6, wherein the diameter of the secondary mirror is 30-38cm.
8. The long working distance, high resolution video microscope for biological observation according to claim 7, wherein the aperture stop has a diameter of 38-42mm and the central circular hole has a diameter of 16-20mm.
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| CN201810357221.2A CN108279491B (en) | 2018-04-19 | 2018-04-19 | Long working distance and high resolution video microscope for biological observation |
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| CN201810357221.2A CN108279491B (en) | 2018-04-19 | 2018-04-19 | Long working distance and high resolution video microscope for biological observation |
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| CN108279491B true CN108279491B (en) | 2024-04-16 |
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|---|---|---|---|---|
| GB179529A (en) * | 1921-05-07 | 1923-02-22 | Zeiss Carl | Spherically, chromatically and astigmatically corrected telephoto objective |
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| US4523816A (en) * | 1983-08-12 | 1985-06-18 | Vivitar Corporation | Catadioptric lens |
| US5139342A (en) * | 1990-01-18 | 1992-08-18 | Olympus Optical Co., Ltd. | Photographic lens system |
| US5973859A (en) * | 1997-11-18 | 1999-10-26 | Asahi Kogaku Kogyo Kabushiki Kaisha | Apochromatic lens system |
| CN101354477A (en) * | 2008-06-18 | 2009-01-28 | 南开大学 | Focusing lens for dual-wavelength OCT system |
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2018
- 2018-04-19 CN CN201810357221.2A patent/CN108279491B/en active Active
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|---|---|---|---|---|
| GB179529A (en) * | 1921-05-07 | 1923-02-22 | Zeiss Carl | Spherically, chromatically and astigmatically corrected telephoto objective |
| GB536556A (en) * | 1939-10-28 | 1941-05-19 | Kodak Ltd | Improvements in or relating to telephoto objectives |
| US4523816A (en) * | 1983-08-12 | 1985-06-18 | Vivitar Corporation | Catadioptric lens |
| US5139342A (en) * | 1990-01-18 | 1992-08-18 | Olympus Optical Co., Ltd. | Photographic lens system |
| US5973859A (en) * | 1997-11-18 | 1999-10-26 | Asahi Kogaku Kogyo Kabushiki Kaisha | Apochromatic lens system |
| CN101354477A (en) * | 2008-06-18 | 2009-01-28 | 南开大学 | Focusing lens for dual-wavelength OCT system |
Non-Patent Citations (2)
| Title |
|---|
| "细胞工厂"显微监测装置的光学设计;李琦等;中国激光;第第41卷卷(第第10期期);１０１６００２－１页至１０１６００２－６页 * |
| 李琦等."细胞工厂"显微监测装置的光学设计.中国激光.第第41卷卷(第第10期期),１０１６００２－１页至１０１６００２－６页. * |
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| CN108279491A (en) | 2018-07-13 |
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