CN108279491A - A kind of long reach, high-resolution videomicroscopy for biology observation - Google Patents
A kind of long reach, high-resolution videomicroscopy for biology observation Download PDFInfo
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- CN108279491A CN108279491A CN201810357221.2A CN201810357221A CN108279491A CN 108279491 A CN108279491 A CN 108279491A CN 201810357221 A CN201810357221 A CN 201810357221A CN 108279491 A CN108279491 A CN 108279491A
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- 238000010865 video microscopy Methods 0.000 title claims abstract description 41
- 239000011248 coating agent Substances 0.000 claims description 12
- 238000000576 coating method Methods 0.000 claims description 12
- 238000002310 reflectometry Methods 0.000 claims description 11
- 230000003287 optical effect Effects 0.000 claims description 6
- 238000007747 plating Methods 0.000 claims description 5
- 238000005452 bending Methods 0.000 claims description 3
- 230000005499 meniscus Effects 0.000 claims description 3
- 230000004075 alteration Effects 0.000 abstract description 20
- 230000000903 blocking effect Effects 0.000 abstract description 3
- 238000004113 cell culture Methods 0.000 abstract description 3
- 239000011521 glass Substances 0.000 description 9
- 238000013461 design Methods 0.000 description 8
- 239000000463 material Substances 0.000 description 7
- 238000003384 imaging method Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 201000009310 astigmatism Diseases 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 206010010071 Coma Diseases 0.000 description 1
- 101000713585 Homo sapiens Tubulin beta-4A chain Proteins 0.000 description 1
- 241000700608 Sagitta Species 0.000 description 1
- 102100036788 Tubulin beta-4A chain Human genes 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000005331 crown glasses (windows) Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000005308 flint glass Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
- G02B21/04—Objectives involving mirrors
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
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Abstract
The present invention provides a kind of long reach, high-resolution videomicroscopy for biology observation, the videomicroscopy includes forming lister's objective group by preceding group of balsaming lens and rear group balsaming lens, Cassegrain's mirror system is formed by secondary mirror and principal reflection mirror, aperture diaphragm is set before lister's objective group, eliminates the part not being imaged generated due to blocking for secondary mirror；By Cassegrain's mirror system in videomicroscopy, due to the presence of principal reflection mirror, its bore can be sufficiently large, can receive energy as much as possible and ensure illuminance, improve numerical aperture, improve resolution ratio, Cassegrain's mirror system does not have aberration simultaneously, to eliminate aberration, ensures object space resolution ratio, there is high-resolution while to ensure long reach, can understand observation cell culture；Videomicroscopy provided by the invention in 400 700nm band operations, operating distance can reach 100mm, numerical aperture 0.38mm, and object space sight field is 1.2mm.
Description
Technical field
The invention belongs to biomedical engineering fields, and in particular to a kind of long reach, high score for biology observation
The videomicroscopy of resolution.
Background technology
The industrial circles such as videomicroscopy is currently widely used for machine vision, and reading measures, due to it by resolution ratio and
The limitation of operating distance is very few in biomedical sector application.The biology laboratory of pharmaceutical manufacturer and scientific research institutions is at present
Cell, inverted microscope high resolution, but its operating distance very little, operating distance are observed using traditional inverted microscope
Only 0.1-3mm.It is to sum up sayed, at present inverted microscope high resolution on the market, but operating distance is short, industrial video is micro-
Mirror work distance, resolution ratio are low；It is generally incubated bottle thickness 30mm, per layer thickness 20mm, tilting observation working distance needs cell factory
Will be in 80mm or more, the height of the cell fermentation tank of different size is in 18mm-100mm etc., therefore inversion on the market is micro-
Mirror and industrial video microscope cannot be directly used to thin in the culture dishes such as observation culture bottle, cell factory and cell fermentation tank
Born of the same parents.
Invention content
In order to solve to cannot be directly used in field of biological pharmacy observe using traditional inverted microscope in the prior art
The problem of cell in culture bottle, cell factory and cell fermentation tank, the object of the present invention is to provide a kind of long reach,
The completely new microcobjective that high-resolution Liszt's type object lens are combined with Cassegrain's speculum, promotes videomicroscopy
Operating distance and resolution ratio facilitate the observation of field of biological pharmacy, ensure pharmaceutical manufacturer's quality monitoring, avoid artificial pollution, entirely
Improve enterprises production efficiency in face.
The present invention provides a kind of long reach, high-resolution videomicroscopy for biology observation, the video
Microscope edge includes successively from front to back with optical axis direction：Aperture diaphragm, preceding group of balsaming lens organize balsaming lens afterwards, and tablet is secondary
Speculum and principal reflection mirror, the secondary mirror are fixedly connected with the tablet, and the principal reflection mirror is plano-concave spherical lens, institute
It states plano-concave spherical lens and is provided centrally with through-hole, the concave surface of the plano-concave spherical lens is close to secondary mirror.
The present invention introduces Cassegrain reflective system in videomicroscopy, the principal reflection mirror in Cassegrain reflective system
Bore can do sufficiently large, so as to receive more energy, ensure illuminance.The increasing of usual bore can cause side
Edge aberration leads to be imaged unintelligible, and the side that the present invention is combined using Cassegrain reflective system and Liszt's type objective lens
Formula reduces the number of optical component, eliminates spherical aberration, aberration correction.
The present invention provides videomicroscopy and constitutes lister's objective group by preceding group of balsaming lens and rear group balsaming lens, by secondary
Speculum and the principal reflection mirror of annular constitute Cassegrain's mirror system, and aperture diaphragm is set to lister's objective by the present invention
Group front, this structure design can eliminate the part not being imaged generated due to blocking for secondary mirror.
Further, the round reflectance coating of center plating on the aperture diaphragm surface, the edge on surface are coated with annular anti-reflection film,
The reflectivity of the reflectivity film is 94% or more, and the transmitance of the anti-reflection film is 98.6% or more.It is provided by the invention to regard
Frequency microscope plates ring at the round high reflection film of the center of aperture diaphragm plating, the edge on periphery, that is, aperture diaphragm surface of reflectance coating
The anti-reflection film of shape further limits the rim ray of system, spherical aberration corrector.
Further, the preceding group balsaming lens includes the first lens and the second lens, and first lens are positive light focus
Lens are spent, the second lens are negative-power lenses, and it includes the third lens and the 4th lens, the third to organize balsaming lens after described
Lens are negative-power lenses, and the 4th lens are positive power lens.
Further, first lens, the second lens, the third lens and the 4th lens are meniscus lens, and described
One concave lens surface close to the aperture diaphragm, first lens, the second lens, the third lens and the 4th lens bending direction
Unanimously, the convex surface of first lens and the fitting of the concave surface of second lens, the convex surface of the third lens and the described 4th
The concave surface of lens is bonded.
Further, the centre of second lens and the third lens is provided with field stop.
Long reach, the high-resolution video microscope of the present invention includes 4 pieces of lens, is followed successively by the first lens, the
Two lens, the third lens and the 4th lens are sequentially the first lens from object plane direction：Positive power lens；Second lens：Negative light
Power lenses；The third lens：Negative-power lenses；4th lens：Positive power lens.First lens and the second lens are one
Group, the third lens and the 4th lens are another group, and two groups of balsaming lens are similar to double gauss structure, and a visual field light is added between microscope group
Door screen limits incident ray height, and second and the third lens convex surface bend towards field stop, is conducive to correct astigmatism and place, and the
One lens, the second lens, the third lens, the 4th lens are a positive negative-power lenses, glued recoverable spherical aberration.Two groups saturating
Mirror is conducive to disperse focal power burden, corrects off-axis point senior aberration at symmetrical double gauss structure.
Further, the Abbe number of first lens is 70.13 or more, and the Abbe number of the third lens is 64.11
More than, the refractive index of second lens is 1.80 or more, and the refractive index of the 4th lens is 1.78 or more.Abbe number is color
Dissipate the inverse of coefficient, the crown glass that the first lens of the invention and the third lens select abbe number small, the second lens and the 4th
The flint glass that lens select reflectivity high, it is this to be designed like clear, high resolution.
Further, the secondary mirror is planoconvex spotlight, and the plane of the planoconvex spotlight is glued with the tablet, described
Convex surface is coated with reflectance coating on the principal reflection mirror, the planoconvex spotlight, and the reflectivity of the reflectance coating is 93% or more, high
The reflectance coating of reflectivity further promotes to improve imaging definition.
Include principal reflection mirror and secondary mirror in the videomicroscopy of the present invention, secondary mirror is plano-convex spherical mirror and tablet
It is mutually glued, ensure that concentricity, principal reflection mirror are plano-concave spherical lens, the plano-concave spherical lens is provided centrally with through-hole, described
The concave surface of plano-concave spherical lens is close to secondary mirror.Secondary mirror is fixed on by way of glued on tablet in the present invention, is put down
Plate selects optical material k9 glass, and very thin adhesive is coated on k9 glass, then secondary mirror is fixed on tablet, this
Sample avoids tablet and is in the light, and ensures the illuminance of imaging.Compared with traditional A-frame fixed form, middle light of the invention
Transmitance is high, and imaging illuminance is sufficient.
Further, a diameter of 80-100mm of the principal reflection mirror, the through-hole at the principal reflection mirror center it is a diameter of
20-24mm, further, a diameter of 30-38cm of the secondary mirror.
Further, a diameter of 38-42mm of the aperture diaphragm, a diameter of 16-20mm of center hole.
The service band of videomicroscopy provided by the invention is 400-700nm, and operating distance is numerical value within 100mm
Aperture is 0.38mm, and object space linear field is 1.2mm.
Beneficial effects of the present invention are：
Lister's objective group is formed by preceding group of balsaming lens and rear group balsaming lens in videomicroscopy provided by the invention,
Cassegrain's mirror system is formed by secondary mirror and principal reflection mirror, aperture diaphragm is set before lister's objective group, is disappeared
Except the part not being imaged generated due to blocking for secondary mirror, lister's objective and Cassegrain's mirror system phase are utilized
In conjunction with mode design microscope, by Cassegrain's mirror system in microscope, due to the presence of principal reflection mirror, primary mirror
Bore can be sufficiently large, energy as much as possible can be received and ensure illuminance, improve numerical aperture, improve resolution ratio, together
When Cassegrain's mirror system there is no aberration, so as to eliminate aberration, ensure object space resolution ratio, ensure farm labourer to realize
Make apart from while have high-resolution, can understand observation cell culture；Videomicroscopy provided by the invention in 400-
700nm band operations, operating distance can reach 100mm, and numerical aperture NA is 0.38mm, and object space sight field is 1.2mm.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
Obtain other attached drawings according to these attached drawings.
Fig. 1 is the structural schematic diagram of videomicroscopy provided by the invention；
Fig. 2 is the MTF transfer curve figures of videomicroscopy provided by the invention；
Fig. 3 is the point range figure of the videomicroscopy of the present invention.
1, aperture diaphragm in figure；2, preceding group of balsaming lens；3, balsaming lens is organized afterwards；4, tablet；5, secondary mirror；6, main anti-
Penetrate mirror；7, through-hole；8, the first lens；9, the second lens；10, the third lens；11, the 4th lens；12, field stop；13, object
Face.
Specific implementation mode
To make the object, technical solutions and advantages of the present invention clearer, technical scheme of the present invention will be carried out below
Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work
Other embodiment belongs to the range that the present invention is protected.
Long reach, the high-resolution videomicroscopy that the present invention provides a kind of for biology observation, it is described aobvious
Micro mirror edge includes once from front to back with optical axis direction：Aperture diaphragm 1, preceding group of balsaming lens 2, rear group balsaming lens 3, tablet 4,
Secondary mirror 5 and principal reflection mirror 6, the secondary mirror 5 are fixedly connected with the tablet 4, and the principal reflection mirror 6 is plano-concave spherical surface
Lens, the plano-concave spherical lens are provided centrally with through-hole 7, and the concave surface of the plano-concave spherical lens is close to secondary mirror 5.This hair
Lister's objective group is formed by preceding group of balsaming lens 2 and rear group balsaming lens 3 in the videomicroscopy of bright offer, by secondary mirror
5 and principal reflection mirror 6 form Cassegrain's mirror system, be combined with Cassegrain's mirror system using lister's objective
Mode designs videomicroscopy, by Cassegrain's mirror system in videomicroscopy, due to the presence of principal reflection mirror 6,
The bore of principal reflection mirror mirror 6 can be sufficiently large, can receive energy as much as possible and ensure illuminance, improve numerical aperture, carry
High-resolution, while Cassegrain's mirror system does not have aberration, so as to eliminate aberration, ensures object space resolution ratio, to
Ensure that there is high-resolution while long reach, can understand observation cell culture.Videomicroscopy provided by the invention,
Service band is 400-700nm, and operating distance is numerical aperture 0.38mm within 100mm, and object space linear field is 1.2mm.
Optionally embodiment, the round reflectance coating of center plating on 1 surface of the aperture diaphragm, the edge on surface is coated with
The reflectivity of annular anti-reflection film, the reflectivity film is 94% or more, and the transmitance of the anti-reflection film is 98.6% or more.This hair
Bright offer videomicroscopy constitutes lister's objective group by preceding group of balsaming lens 2 and rear group balsaming lens 3, by 5 He of secondary mirror
The principal reflection mirror 6 of annular constitutes Cassegrain's mirror system, before aperture diaphragm 1 is set to lister's objective group by the present invention
Face, and plate round high reflection film, the edge plating on 1 surface of periphery, that is, aperture diaphragm of reflectance coating at the center of aperture diaphragm 1
The anti-reflection film of annular, this structure design can eliminate the part not being imaged blocked and generated due to secondary mirror 5, and
The rim ray of limitation system, spherical aberration corrector.
Optionally embodiment, it is described before group balsaming lens 2 include the first lens 8 and the second lens 9, described first
Lens 8 are positive power lens, and the second lens 9 are negative-power lenses, and it includes 10 He of the third lens that balsaming lens 3 is organized after described
4th lens 11, the third lens 10 are negative-power lenses, and the 4th lens 11 are positive power lens.
Optionally embodiment, first lens 8, the second lens 9, the third lens 10 and the 4th lens 11 are
Meniscus lens, 8 concave surface of the first lens is close to the aperture diaphragm 1, first lens 8, the second lens 9, the third lens
10 is consistent with the bending direction of the 4th lens 11, the concave surface fitting of the convex surface of first lens 8 and second lens 9, institute
State the convex surface of the third lens 10 and the concave surface fitting of the 4th lens 11.
As optional embodiment, field stop 12 is provided among second lens 9 and the third lens 10.
The present invention long reach, high-resolution videomicroscopy include 4 pieces of lens, be followed successively by the first lens 8,
Second lens 9, the third lens 10 and the 4th lens 11 are sequentially the first lens 8 from 13 direction of object plane：Positive power lens；The
Two lens 9：Negative-power lenses；The third lens 10：Negative-power lenses；4th lens 11：Positive power lens.First lens
8 and second lens 9 be one group, the third lens 10 and the 4th lens 11 are another group, and two groups of balsaming lens are similar to double gauss knot
Structure adds a field stop 12 between microscope group, limits incident ray height, and second and 10 convex surface of the third lens bend towards field stop
12, be conducive to correct astigmatism and place, and the first lens 8, the second lens 9, the third lens 10, the 4th lens 11 are one positive one
Negative-power lenses, glued recoverable spherical aberration.Two groups of lens are conducive to disperse focal power burden, school at symmetrical double gauss structure
Positive off-axis point senior aberration.
As optional embodiment, the plane of the planoconvex spotlight is glued with the tablet 4, and the convex surface is close to described
Principal reflection mirror 6 is coated with reflectance coating on the planoconvex spotlight, and the reflectivity of the reflectance coating is 93% or more, high reflectance it is anti-
Film is penetrated further to promote to improve imaging definition.
The present invention videomicroscopy in include principal reflection mirror 6 and secondary mirror 5, secondary mirror 5 be plano-convex spherical mirror with
4 phase of tablet is glued, ensures that concentricity, principal reflection mirror 6 are plano-concave spherical lens, the plano-concave spherical lens is provided centrally with through-hole
7, the concave surface of the plano-concave spherical lens is close to secondary mirror 5.Secondary mirror 5 is fixed on flat by way of glued in the present invention
On plate 4, tablet 4 selects optical material k9 glass, and very thin adhesive is coated on k9 glass, then fixes secondary mirror 5
On tablet 4, this avoid tablets 4 to be in the light, and ensures the illuminance of imaging.Compared with traditional A-frame fixed form,
The transmitance of the middle light of the present invention is high, and imaging illuminance is sufficient.
As optional embodiment, a diameter of 80-100mm of the principal reflection mirror 6,6 center of the principal reflection mirror
A diameter of 20-24mm of through-hole 7.
As optional embodiment, a diameter of 30-38mm of the secondary mirror 5.
As optional embodiment, a diameter of 38-42mm of the aperture diaphragm 1, a diameter of 16- of center hole
20mm。
A kind of specific design scheme presented below, videomicroscopy of the invention set in comprising principal reflection mirror 6 and time anti-
Mirror 5 is penetrated, secondary mirror is that planoconvex spotlight is glued with 4 phase of tablet, ensures that concentricity, principal reflection mirror 6 are plano-concave spherical lens, concave surface is bent towards
Object plane 13, bore 80-100mm, intermediate opened Circularhole diameter are 20-24mm.
Table 1 is described microscopical more particularly to parameter.Table 2 gives the farm labourer provided by the present invention for biology observation
Make the design parameter value of distance, high-resolution videomicroscopy per a piece of lens, what " radius " column indicated is each surface
Corresponding spherical radius, what " thickness/interval " indicated is the airspace (center of lens between the thickness of eyeglass and two microscope groups
Axial distance)." material " refers to corresponding lens materials, and " effective clear aperature " refers to the practical ray height in the face.In table
G1 represents the first lens 8, and G2 represents the second lens 9, and G3 represents the third lens 10, and G4 represents the 4th lens 11.
Preceding group of balsaming lens 2 includes G1 and G2, and rear balsaming lens 3 of organizing includes G3 and G4, for organizing balsaming lens 2 in the past,
It is divided into the faces front surface T1, the faces rear surface T2, the spherical radius that the spherical radius of T1 is -291.655, T2 is that -111.517, T1 is arrived
The axial distance of object plane 13 is 100mm (operating distance), and the axial distance in the faces T1 to T2 is G1 thickness of glass 10mm, glass material
For H-QK3.The effective clear aperature in the faces T1 is 40.5mm, effective clear aperature 43.3mm of rear surface T2.
Since G1, G2 are preceding group of balsaming lens 2, so the spherical radius of the front surface T3 of G2 is -111.517, rear surface
T4 spherical radius is -149.41, and the axial distance of T3 to T4 is G2 thickness of glass 8.5mm, glass material H-ZLAF1.The faces T3
Effective clear aperature (T3=T2) is 43.3mm, effective clear aperature 46.7mm of rear surface T4.Each parameter of remaining in table 2 according to
G1, G2 and so on.
The spherical radius of principal reflection mirror 6 and time transmitting mirror is -141.6, -96.84, and principal reflection mirror 6 is plano-concave spherical lens,
Concave surface bends towards object plane 13, and secondary mirror is planoconvex spotlight, and convex surface bends towards primary mirror.Glass material is for H-K9L, and effective aperture is respectively
87.8mm and 37.8mm.Specific structural design drawing is shown in Fig. 1.
What Fig. 2 was indicated is the MTF transfer curve figures of the videomicroscopy of the present invention.
Had a look at out from the function curve of Fig. 2, at cutoff frequency 100lp/mm, average transfer functions reach 0.16 with
On, meridian is overlapped with sagitta of arc line preferably, all close to diffraction limit.Illustrate that system has good image quality.
What Fig. 3 was indicated is the point range figure of the videomicroscopy of the present invention.
According to the upper data of figure it is found that root mean square disc of confusion RMS is less than Airy radius 8.952um, integral dispersion spot GEO
Less than Airy radius, illustrate that systemic resolution is very high.Microscope belongs to small aberration system and re-graduation in aberration correction
Positive spherical aberration, coma and chromatism of position, and can be seen that from point range figure, three kinds of aberration very littles, tolerance for aberration is respectively less than by calculating,
Design result is reasonable.
Table 1
| Operating distance (L) | 100mm |
| Numerical aperture NA | 0.38mm |
| Object space linear field | 1.2mm |
| Service band | 400-700nm |
| Tube length | 625mm |
| Enlargement ratio β | -5× |
Table 2
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of long reach, high-resolution videomicroscopy for biology observation, which is characterized in that along same optical axis side
To including successively from front to back：Aperture diaphragm, preceding group of balsaming lens, organizes balsaming lens, tablet, secondary mirror and principal reflection afterwards
Mirror, the secondary mirror are fixedly connected with the tablet, and the principal reflection mirror is plano-concave spherical lens, the plano-concave spherical lens
It is provided centrally with through-hole, the concave surface of the plano-concave spherical lens is close to secondary mirror.
2. long reach, the high-resolution videomicroscopy according to claim 1 for biology observation, feature
It is, the round reflectance coating of center plating on the aperture diaphragm surface, the edge on surface is coated with annular anti-reflection film, the reflectivity film
Reflectivity be 94% or more, the transmitance of the anti-reflection film is 98.6% or more.
3. long reach, the high-resolution videomicroscopy according to claim 2 for biology observation, feature
It is, it includes the first lens and the second lens that balsaming lens is organized before described, and first lens are positive power lens, and second thoroughly
Mirror is negative-power lenses, and it includes the third lens and the 4th lens that balsaming lens is organized after described, and the third lens are negative light focus
Lens are spent, the 4th lens are positive power lens.
4. long reach, the high-resolution videomicroscopy according to claim 3 for biology observation, feature
It is, first lens, the second lens, the third lens and the 4th lens are meniscus lens, and first concave lens surface is leaned on
The nearly aperture diaphragm, first lens, the second lens, the third lens are consistent with the bending direction of the 4th lens, and described the
The concave surface patch of the convex surface of one lens and the fitting of the concave surface of second lens, the convex surface of the third lens and the 4th lens
It closes.
5. long reach, the high-resolution videomicroscopy according to claim 4 for biology observation, feature
It is, the centre of second lens and the third lens is provided with field stop.
6. long reach, the high-resolution videomicroscopy according to claim 5 for biology observation, feature
It is, the Abbe number of first lens is 70.13 or more, and the Abbe number of the third lens is 64.11 or more, described second
The refractive index of lens is 1.80 or more, and the refractive index of the 4th lens is 1.78 or more.
7. long reach, the high-resolution videomicroscopy according to claim 6 for biology observation, feature
It is, the secondary mirror is planoconvex spotlight, and the plane of the planoconvex spotlight is glued with the tablet, and the convex surface is close to described
Principal reflection mirror is coated with reflectance coating on the planoconvex spotlight, and the reflectivity of the reflectance coating is 93% or more.
8. long reach, the high-resolution videomicroscopy according to claim 7 for biology observation, feature
It is, a diameter of 80-100mm of the principal reflection mirror, a diameter of 20-24mm of the through-hole at the principal reflection mirror center.
9. long reach, the high-resolution videomicroscopy according to claim 8 for biology observation, feature
It is, a diameter of 30-38cm of the secondary mirror.
10. long reach, the high-resolution videomicroscopy according to claim 9 for biology observation, special
Sign is, a diameter of 38-42mm of the aperture diaphragm, a diameter of 16-20mm of center hole.
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| CN201810357221.2A CN108279491B (en) | 2018-04-19 | 2018-04-19 | Long working distance and high resolution video microscope for biological observation |
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| CN201810357221.2A CN108279491B (en) | 2018-04-19 | 2018-04-19 | Long working distance and high resolution video microscope for biological observation |
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| CN108279491B CN108279491B (en) | 2024-04-16 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB179529A (en) * | 1921-05-07 | 1923-02-22 | Zeiss Carl | Spherically, chromatically and astigmatically corrected telephoto objective |
| GB536556A (en) * | 1939-10-28 | 1941-05-19 | Kodak Ltd | Improvements in or relating to telephoto objectives |
| US4523816A (en) * | 1983-08-12 | 1985-06-18 | Vivitar Corporation | Catadioptric lens |
| US5139342A (en) * | 1990-01-18 | 1992-08-18 | Olympus Optical Co., Ltd. | Photographic lens system |
| US5973859A (en) * | 1997-11-18 | 1999-10-26 | Asahi Kogaku Kogyo Kabushiki Kaisha | Apochromatic lens system |
| CN101354477A (en) * | 2008-06-18 | 2009-01-28 | 南开大学 | Focusing lens for dual-wavelength OCT system |
-
2018
- 2018-04-19 CN CN201810357221.2A patent/CN108279491B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB179529A (en) * | 1921-05-07 | 1923-02-22 | Zeiss Carl | Spherically, chromatically and astigmatically corrected telephoto objective |
| GB536556A (en) * | 1939-10-28 | 1941-05-19 | Kodak Ltd | Improvements in or relating to telephoto objectives |
| US4523816A (en) * | 1983-08-12 | 1985-06-18 | Vivitar Corporation | Catadioptric lens |
| US5139342A (en) * | 1990-01-18 | 1992-08-18 | Olympus Optical Co., Ltd. | Photographic lens system |
| US5973859A (en) * | 1997-11-18 | 1999-10-26 | Asahi Kogaku Kogyo Kabushiki Kaisha | Apochromatic lens system |
| CN101354477A (en) * | 2008-06-18 | 2009-01-28 | 南开大学 | Focusing lens for dual-wavelength OCT system |
Non-Patent Citations (1)
| Title |
|---|
| 李琦等: ""细胞工厂"显微监测装置的光学设计", 中国激光, vol. 41, no. 10 * |
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