CN208060826U - A kind of long reach, high-resolution videomicroscopy for biology observation - Google Patents
A kind of long reach, high-resolution videomicroscopy for biology observation Download PDFInfo
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- CN208060826U CN208060826U CN201820584219.4U CN201820584219U CN208060826U CN 208060826 U CN208060826 U CN 208060826U CN 201820584219 U CN201820584219 U CN 201820584219U CN 208060826 U CN208060826 U CN 208060826U
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- lens
- videomicroscopy
- resolution
- mirror
- long reach
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Abstract
The utility model provides a kind of long reach, high-resolution videomicroscopy for biology observation, the videomicroscopy includes forming lister's objective group by preceding group of balsaming lens and rear group balsaming lens, Cassegrain's mirror system is formed by secondary mirror and principal reflection mirror, aperture diaphragm is set before lister's objective group, eliminates the part not being imaged generated due to blocking for secondary mirror;Due to the presence of principal reflection mirror, its bore can be sufficiently large, energy as much as possible can be received and ensure illuminance, numerical aperture is improved, improves resolution ratio, while Cassegrain's mirror system does not have aberration, to eliminate aberration, ensure object space resolution ratio, there is high-resolution while to ensure long reach, can understand observation cell culture;Videomicroscopy provided by the utility model in 400-700nm band operations, operating distance can reach 100mm, numerical aperture 0.38mm, and object space sight field is 1.2mm.
Description
Technical field
The utility model belongs to biomedical engineering field, and in particular to it is a kind of for biology observation long reach,
High-resolution videomicroscopy.
Background technology
The industrial circles such as videomicroscopy is currently widely used for machine vision, and reading measures, due to it by resolution ratio and
The limitation of operating distance is very few in biomedical sector application.The biology laboratory of pharmaceutical manufacturer and scientific research institutions is at present
Cell, inverted microscope high resolution, but its operating distance very little, operating distance are observed using traditional inverted microscope
Only 0.1-3mm.It is to sum up sayed, at present inverted microscope high resolution on the market, but operating distance is short, industrial video is micro-
Mirror work distance, resolution ratio are low;It is generally incubated bottle thickness 30mm, per layer thickness 20mm, tilting observation working distance needs cell factory
Will be in 80mm or more, the height of the cell fermentation tank of different size is in 18mm-100mm etc., therefore inversion on the market is micro-
Mirror and industrial video microscope cannot be directly used to thin in the culture dishes such as observation culture bottle, cell factory and cell fermentation tank
Born of the same parents.
Utility model content
In order to solve to cannot be directly used in field of biological pharmacy observe using traditional inverted microscope in the prior art
The problem of cell in culture bottle, cell factory and cell fermentation tank, the purpose of this utility model is to provide a kind of long work
It is aobvious to promote video for the completely new microcobjective that distance, high-resolution Liszt's type object lens are combined with Cassegrain's speculum
The operating distance and resolution ratio of micro mirror facilitate the observation of field of biological pharmacy, ensure pharmaceutical manufacturer's quality monitoring, avoid artificial dirt
Dye, improves enterprises production efficiency comprehensively.
The utility model provides a kind of long reach, high-resolution videomicroscopy for biology observation, described
Videomicroscopy edge includes successively from front to back with optical axis direction:Aperture diaphragm, preceding group of balsaming lens, organizes balsaming lens afterwards, puts down
Plate, secondary mirror and principal reflection mirror, the secondary mirror are fixedly connected with the tablet, and the principal reflection mirror is that plano-concave spherical surface is saturating
Mirror, the plano-concave spherical lens are provided centrally with through-hole, and the concave surface of the plano-concave spherical lens is close to secondary mirror.
The utility model introduces Cassegrain reflective system in videomicroscopy, and the master in Cassegrain reflective system is anti-
Penetrating the bore of mirror can do sufficiently large, so as to receive more energy, ensure illuminance.The increasing of usual bore can draw
Rise marginal aberration, cause to be imaged it is unintelligible, and the utility model use Cassegrain reflective system and Liszt's type objective lens phase
In conjunction with mode, reduce the number of optical component, eliminate spherical aberration, aberration correction.
The utility model provides videomicroscopy and constitutes lister's objective group by preceding group of balsaming lens and rear group balsaming lens,
Cassegrain's mirror system is constituted by the principal reflection mirror of secondary mirror and annular, aperture diaphragm is set to Lee by the utility model
Before this special objective lens, this structure design can eliminate the part not being imaged generated due to blocking for secondary mirror.
Further, the round reflectance coating of center plating on the aperture diaphragm surface, the edge on surface are coated with annular anti-reflection film,
The reflectivity of the reflectivity film is 94% or more, and the transmitance of the anti-reflection film is 98.6% or more.The utility model provides
Videomicroscopy at the round high reflection film of the center of aperture diaphragm plating, the edge on periphery, that is, aperture diaphragm surface of reflectance coating
The anti-reflection film of annular is plated, the rim ray of system, spherical aberration corrector are further limited.
Further, the preceding group balsaming lens includes the first lens and the second lens, and first lens are positive light focus
Lens are spent, the second lens are negative-power lenses, and it includes the third lens and the 4th lens, the third to organize balsaming lens after described
Lens are negative-power lenses, and the 4th lens are positive power lens.
Further, first lens, the second lens, the third lens and the 4th lens are meniscus lens, and described
One concave lens surface close to the aperture diaphragm, first lens, the second lens, the third lens and the 4th lens bending direction
Unanimously, the convex surface of first lens and the fitting of the concave surface of second lens, the convex surface of the third lens and the described 4th
The concave surface of lens is bonded.
Further, the centre of second lens and the third lens is provided with field stop.
The long reach, high-resolution video microscope of the utility model include 4 pieces of lens, are followed successively by first thoroughly
Mirror, the second lens, the third lens and the 4th lens are sequentially the first lens from object plane direction:Positive power lens;Second thoroughly
Mirror:Negative-power lenses;The third lens:Negative-power lenses;4th lens:Positive power lens.First lens and second are thoroughly
Mirror is one group, and the third lens and the 4th lens are another group, and two groups of balsaming lens are similar to double gauss structure, and one is added between microscope group
Field stop limits incident ray height, and second and the third lens convex surface bend towards field stop, is conducive to correct astigmatism and field
Area, and the first lens, the second lens, the third lens, the 4th lens are a positive negative-power lenses, glued recoverable ball
Difference.Two groups of lens are conducive to disperse focal power burden, correct off-axis point senior aberration at symmetrical double gauss structure.
Further, the Abbe number of first lens is 70.13 or more, and the Abbe number of the third lens is 64.11
More than, the refractive index of second lens is 1.80 or more, and the refractive index of the 4th lens is 1.78 or more.Abbe number is color
Dissipate the inverse of coefficient, the crown glass that first lens and the third lens of the utility model select abbe number small, the second lens
High flint glass, this to be designed like clear, high resolution with the 4th lens selection reflectivity.
Further, the secondary mirror is planoconvex spotlight, and the plane of the planoconvex spotlight is glued with the tablet, described
Convex surface is coated with reflectance coating on the principal reflection mirror, the planoconvex spotlight, and the reflectivity of the reflectance coating is 93% or more, high
The reflectance coating of reflectivity further promotes to improve imaging definition.
In the videomicroscopy of the utility model include principal reflection mirror and secondary mirror, secondary mirror be plano-convex spherical mirror with
Tablet is mutually glued, ensures that concentricity, principal reflection mirror are plano-concave spherical lens, and the plano-concave spherical lens is provided centrally with through-hole,
The concave surface of the plano-concave spherical lens is close to secondary mirror.Secondary mirror is fixed on flat by way of glued in the utility model
On plate, tablet selects optical material k9 glass, and very thin adhesive is coated on k9 glass, is then fixed on secondary mirror flat
On plate, this avoid tablets to be in the light, and ensures the illuminance of imaging.Compared with traditional A-frame fixed form, this practicality
The transmitance of novel middle light is high, and imaging illuminance is sufficient.
Further, a diameter of 80-100mm of the principal reflection mirror, the through-hole at the principal reflection mirror center it is a diameter of
20-24mm。
Further, a diameter of 38-42mm of the aperture diaphragm, a diameter of 16-20mm of center hole.
The service band of videomicroscopy provided by the utility model be 400-700nm, operating distance be 100mm within,
Numerical aperture is 0.38mm, and object space linear field is 1.2mm.
The beneficial effects of the utility model are:
Liszt's object is formed by preceding group of balsaming lens and rear group balsaming lens in videomicroscopy provided by the utility model
Microscope group forms Cassegrain's mirror system by secondary mirror and principal reflection mirror, aperture light is arranged before lister's objective group
Door screen is eliminated the part not being imaged generated due to blocking for secondary mirror, utilizes lister's objective and Cassegrain's speculum
The mode that system is combined designs microscope, Cassegrain's mirror system is used in microscope, due to depositing for principal reflection mirror
The bore of primary mirror can be sufficiently large, can receive energy as much as possible and ensure illuminance, improve numerical aperture, improves point
Resolution, while Cassegrain's mirror system does not have aberration, so as to eliminate aberration, ensures object space resolution ratio, to ensure
There is high-resolution while long reach, can understand observation cell culture;Videomicroscopy provided by the utility model
In 400-700nm band operations, operating distance can reach 100mm, and numerical aperture NA is 0.38mm, and object space sight field is
1.2mm。
Description of the drawings
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, other drawings may also be obtained based on these drawings.
Fig. 1 is the structural schematic diagram of videomicroscopy provided by the utility model;
Fig. 2 is the MTF transfer curve figures of videomicroscopy provided by the utility model;
Fig. 3 is the point range figure of the videomicroscopy of the utility model.
1, aperture diaphragm in figure;2, preceding group of balsaming lens;3, balsaming lens is organized afterwards;4, tablet;5, secondary mirror;6, main anti-
Penetrate mirror;7, through-hole;8, the first lens;9, the second lens;10, the third lens;11, the 4th lens;12, field stop;13, object
Face.
Specific implementation mode
It, below will be to the technology of the utility model to keep the purpose of this utility model, technical solution and advantage clearer
Scheme is described in detail.Obviously, the described embodiments are only a part of the embodiments of the utility model, rather than all
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are not before making creative work
Obtained all other embodiment is put, the range that the utility model is protected is belonged to.
The utility model provides a kind of long reach, high-resolution videomicroscopy for biology observation, institute
State microscope edge includes once from front to back with optical axis direction:Aperture diaphragm 1, preceding group of balsaming lens 2 is rear to organize balsaming lens 3, puts down
Plate 4, secondary mirror 5 and principal reflection mirror 6, the secondary mirror 5 are fixedly connected with the tablet 4, and the principal reflection mirror 6 is plano-concave
Spherical lens, the plano-concave spherical lens are provided centrally with through-hole 7, and the concave surface of the plano-concave spherical lens is close to secondary mirror 5.
Lister's objective group is formed by preceding group of balsaming lens 2 and rear group balsaming lens 3 in videomicroscopy provided by the utility model, by
Secondary mirror 5 and principal reflection mirror 6 form Cassegrain's mirror system, utilize lister's objective and Cassegrain's mirror system
The mode being combined designs videomicroscopy, Cassegrain's mirror system is used in videomicroscopy, due to principal reflection mirror 6
Presence, the bore of principal reflection mirror mirror 6 can be sufficiently large, can receive energy as much as possible and ensure illuminance, improve numerical value
Resolution ratio is improved in aperture, while Cassegrain's mirror system does not have aberration, so as to eliminate aberration, ensures that object space is differentiated
Rate ensures there is high-resolution while long reach to realize, can understand observation cell culture.The utility model carries
The videomicroscopy of confession, service band 400-700nm, operating distance are numerical aperture 0.38mm within 100mm, object space
Linear field is 1.2mm.
Optionally embodiment, the round reflectance coating of center plating on 1 surface of the aperture diaphragm, the edge on surface is coated with
The reflectivity of annular anti-reflection film, the reflectivity film is 94% or more, and the transmitance of the anti-reflection film is 98.6% or more.This reality
Lister's objective group is constituted by preceding group of balsaming lens 2 and rear group balsaming lens 3 with novel offer videomicroscopy, by secondary mirror
5 constitute Cassegrain's mirror system with annular principal reflection mirror 6, and aperture diaphragm 1 is set to Liszt's object by the utility model
Before microscope group, and round high reflection film is plated at the center of aperture diaphragm 1,1 surface of periphery, that is, aperture diaphragm of reflectance coating
The anti-reflection film of edge plating annular, this structure design can eliminate the portion not being imaged blocked and generated due to secondary mirror 5
Point, and limit the rim ray of system, spherical aberration corrector.
Optionally embodiment, it is described before group balsaming lens 2 include the first lens 8 and the second lens 9, described first
Lens 8 are positive power lens, and the second lens 9 are negative-power lenses, and it includes 10 He of the third lens that balsaming lens 3 is organized after described
4th lens 11, the third lens 10 are negative-power lenses, and the 4th lens 11 are positive power lens.
Optionally embodiment, first lens 8, the second lens 9, the third lens 10 and the 4th lens 11 are
Meniscus lens, 8 concave surface of the first lens is close to the aperture diaphragm 1, first lens 8, the second lens 9, the third lens
10 is consistent with the bending direction of the 4th lens 11, the concave surface fitting of the convex surface of first lens 8 and second lens 9, institute
State the convex surface of the third lens 10 and the concave surface fitting of the 4th lens 11.
As optional embodiment, field stop 12 is provided among second lens 9 and the third lens 10.
The long reach of the utility model, high-resolution videomicroscopy include 4 pieces of lens, are followed successively by first thoroughly
Mirror 8, the second lens 9, the third lens 10 and the 4th lens 11 are sequentially the first lens 8 from 13 direction of object plane:Positive light coke is saturating
Mirror;Second lens 9:Negative-power lenses;The third lens 10:Negative-power lenses;4th lens 11:Positive power lens.The
One lens 8 and the second lens 9 are one group, and the third lens 10 and the 4th lens 11 are another group, and two groups of balsaming lens are similar to double
Gaussian structures add a field stop 12 between microscope group, limit incident ray height, and second and 10 convex surface of the third lens bend towards visual field
Diaphragm 12 is conducive to correct astigmatism and place, and the first lens 8, the second lens 9, the third lens 10, the 4th lens 11 are one
A positive negative-power lenses, glued recoverable spherical aberration.It is negative to be conducive to dispersion focal power at symmetrical double gauss structure for two groups of lens
Load corrects off-axis point senior aberration.
As optional embodiment, the plane of the planoconvex spotlight is glued with the tablet 4, and the convex surface is close to described
Principal reflection mirror 6 is coated with reflectance coating on the planoconvex spotlight, and the reflectivity of the reflectance coating is 93% or more, high reflectance it is anti-
Film is penetrated further to promote to improve imaging definition.
Include principal reflection mirror 6 and secondary mirror 5 in the videomicroscopy of the utility model, secondary mirror 5 is plano-convex spherical
Mirror is glued with 4 phase of tablet, ensures that concentricity, principal reflection mirror 6 are plano-concave spherical lens, the plano-concave spherical lens is provided centrally with
Through-hole 7, the concave surface of the plano-concave spherical lens is close to secondary mirror 5.Secondary mirror 5 is by way of glued in the utility model
It is fixed on tablet 4, tablet 4 selects optical material k9 glass, very thin adhesive is coated on k9 glass, then by secondary reflection
Mirror 5 is fixed on tablet 4, and this avoid tablets 4 to be in the light, and ensures the illuminance of imaging.With traditional A-frame fixation side
Formula is compared, and the transmitance of the middle light of the utility model is high, and imaging illuminance is sufficient.
As optional embodiment, a diameter of 80-100mm of the principal reflection mirror 6,6 center of the principal reflection mirror
A diameter of 20-24mm of through-hole 7.
As optional embodiment, a diameter of 30-38mm of the secondary mirror 5.
As optional embodiment, a diameter of 38-42mm of the aperture diaphragm 1, a diameter of 16- of center hole
20mm。
A kind of specific design scheme presented below, the videomicroscopy of the utility model set in include 6 He of principal reflection mirror
Secondary mirror 5, secondary mirror are that planoconvex spotlight is glued with 4 phase of tablet, ensure that concentricity, principal reflection mirror 6 are plano-concave spherical lens, concave surface
Object plane 13, bore 80-100mm are bent towards, intermediate opened Circularhole diameter is 20-24mm.
Table 1 is described microscopical more particularly to parameter.Table 2 give it is provided by the utility model for biology observation
The design parameter value of long reach, high-resolution videomicroscopy per a piece of lens, what " radius " column indicated is each
Spherical radius corresponding to surface, what " thickness/interval " indicated is the airspace (eyeglass between the thickness of eyeglass and two microscope groups
The axial distance at center)." material " refers to corresponding lens materials, and " effective clear aperature " refers to the practical ray height in the face.
G1 represents the first lens 8 in table, and G2 represents the second lens 9, and G3 represents the third lens 10, and G4 represents the 4th lens 11.
Preceding group of balsaming lens 2 includes G1 and G2, and rear balsaming lens 3 of organizing includes G3 and G4, for organizing balsaming lens 2 in the past,
It is divided into the faces front surface T1, the faces rear surface T2, the spherical radius that the spherical radius of T1 is -291.655, T2 is that -111.517, T1 is arrived
The axial distance of object plane 13 is 100mm (operating distance), and the axial distance in the faces T1 to T2 is G1 thickness of glass 10mm, glass material
For H-QK3.The effective clear aperature in the faces T1 is 40.5mm, effective clear aperature 43.3mm of rear surface T2.
Since G1, G2 are preceding group of balsaming lens 2, so the spherical radius of the front surface T3 of G2 is -111.517, rear surface
T4 spherical radius is -149.41, and the axial distance of T3 to T4 is G2 thickness of glass 8.5mm, glass material H-ZLAF1.The faces T3
Effective clear aperature (T3=T2) is 43.3mm, effective clear aperature 46.7mm of rear surface T4.Each parameter of remaining in table 2 according to
G1, G2 and so on.
The spherical radius of principal reflection mirror 6 and time transmitting mirror is -141.6, -96.84, and principal reflection mirror 6 is plano-concave spherical lens,
Concave surface bends towards object plane 13, and secondary mirror is planoconvex spotlight, and convex surface bends towards primary mirror.Glass material is for H-K9L, and effective aperture is respectively
87.8mm and 37.8mm.Specific structural design drawing is shown in Fig. 1.
What Fig. 2 was indicated is the MTF transfer curve figures of the videomicroscopy of the utility model.
Had a look at out from the function curve of Fig. 2, at cutoff frequency 100lp/mm, average transfer functions reach 0.16 with
On, meridian is overlapped with sagitta of arc line preferably, all close to diffraction limit.Illustrate that system has good image quality.
What Fig. 3 was indicated is the point range figure of the videomicroscopy of the utility model.
According to the upper data of figure it is found that root mean square disc of confusion RMS is less than Airy radius 8.952um, integral dispersion spot GEO
Less than Airy radius, illustrate that systemic resolution is very high.Microscope belongs to small aberration system and re-graduation in aberration correction
Positive spherical aberration, coma and chromatism of position, and can be seen that from point range figure, three kinds of aberration very littles, tolerance for aberration is respectively less than by calculating,
Design result is reasonable.
Table 1
Operating distance (L) | 100mm |
Numerical aperture NA | 0.38mm |
Object space linear field | 1.2mm |
Service band | 400-700nm |
Tube length | 625mm |
Enlargement ratio β | -5× |
Table 2
Above description is only a specific implementation of the present invention, but the scope of protection of the utility model is not limited to
In this, any one skilled in the art within the technical scope disclosed by the utility model, can readily occur in variation
Or replace, it should be covered within the scope of the utility model.Therefore, the scope of protection of the utility model should be with the power
Subject to the protection domain that profit requires.
Claims (10)
1. a kind of long reach, high-resolution videomicroscopy for biology observation, which is characterized in that along same optical axis side
To including successively from front to back:Aperture diaphragm, preceding group of balsaming lens, organizes balsaming lens, tablet, secondary mirror and principal reflection afterwards
Mirror, the secondary mirror are fixedly connected with the tablet, and the principal reflection mirror is plano-concave spherical lens, the plano-concave spherical lens
It is provided centrally with through-hole, the concave surface of the plano-concave spherical lens is close to secondary mirror.
2. long reach, the high-resolution videomicroscopy according to claim 1 for biology observation, feature
It is, the round reflectance coating of center plating on the aperture diaphragm surface, the edge on surface is coated with annular anti-reflection film, the reflectance coating
Reflectivity is 94% or more, and the transmitance of the anti-reflection film is 98.6% or more.
3. long reach, the high-resolution videomicroscopy according to claim 2 for biology observation, feature
It is, it includes the first lens and the second lens that balsaming lens is organized before described, and first lens are positive power lens, and second thoroughly
Mirror is negative-power lenses, and it includes the third lens and the 4th lens that balsaming lens is organized after described, and the third lens are negative light focus
Lens are spent, the 4th lens are positive power lens.
4. long reach, the high-resolution videomicroscopy according to claim 3 for biology observation, feature
It is, first lens, the second lens, the third lens and the 4th lens are meniscus lens, and first concave lens surface is leaned on
The nearly aperture diaphragm, first lens, the second lens, the third lens are consistent with the bending direction of the 4th lens, and described the
The concave surface patch of the convex surface of one lens and the fitting of the concave surface of second lens, the convex surface of the third lens and the 4th lens
It closes.
5. long reach, the high-resolution videomicroscopy according to claim 4 for biology observation, feature
It is, the centre of second lens and the third lens is provided with field stop.
6. long reach, the high-resolution videomicroscopy according to claim 5 for biology observation, feature
It is, the Abbe number of first lens is 70.13 or more, and the Abbe number of the third lens is 64.11 or more, described second
The refractive index of lens is 1.80 or more, and the refractive index of the 4th lens is 1.78 or more.
7. long reach, the high-resolution videomicroscopy according to claim 6 for biology observation, feature
It is, the secondary mirror is planoconvex spotlight, and the plane of the planoconvex spotlight is glued with the tablet, and the convex surface is close to described
Principal reflection mirror is coated with reflectance coating on the planoconvex spotlight, and the reflectivity of the reflectance coating is 93% or more.
8. long reach, the high-resolution videomicroscopy according to claim 7 for biology observation, feature
It is, a diameter of 80-100mm of the principal reflection mirror, a diameter of 20-24mm of the through-hole at the principal reflection mirror center.
9. long reach, the high-resolution videomicroscopy according to claim 8 for biology observation, feature
It is, a diameter of 30-38cm of the secondary mirror.
10. long reach, the high-resolution videomicroscopy according to claim 9 for biology observation, special
Sign is, a diameter of 38-42mm of the aperture diaphragm, a diameter of 16-20mm of center hole.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201820584219.4U CN208060826U (en) | 2018-04-19 | 2018-04-19 | A kind of long reach, high-resolution videomicroscopy for biology observation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201820584219.4U CN208060826U (en) | 2018-04-19 | 2018-04-19 | A kind of long reach, high-resolution videomicroscopy for biology observation |
Publications (1)
Publication Number | Publication Date |
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CN208060826U true CN208060826U (en) | 2018-11-06 |
Family
ID=63983629
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CN201820584219.4U Expired - Fee Related CN208060826U (en) | 2018-04-19 | 2018-04-19 | A kind of long reach, high-resolution videomicroscopy for biology observation |
Country Status (1)
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CN (1) | CN208060826U (en) |
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2018
- 2018-04-19 CN CN201820584219.4U patent/CN208060826U/en not_active Expired - Fee Related
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Granted publication date: 20181106 Termination date: 20200419 |