CN108272959A - A kind of herbal mixture and its preparation method and application - Google Patents
A kind of herbal mixture and its preparation method and application Download PDFInfo
- Publication number
- CN108272959A CN108272959A CN201810253967.9A CN201810253967A CN108272959A CN 108272959 A CN108272959 A CN 108272959A CN 201810253967 A CN201810253967 A CN 201810253967A CN 108272959 A CN108272959 A CN 108272959A
- Authority
- CN
- China
- Prior art keywords
- wound
- group
- herbal mixture
- coptis
- radix scutellariae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/718—Coptis (goldthread)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Neurosurgery (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of herbal mixtures and its preparation method and application.The herbal mixture is made of radix scutellariae, the coptis, the bletilla striata and borneol, and mass ratio is 2~20 ︰, 1~15 ︰, 1~10 ︰ 0.1~5.Mutual synergistic function is played between four taste Chinese medicines in herbal mixture of the present invention, is accelerated surface of a wound blood coagulation, antiphlogistic antibacterial, is reduced surface of a wound exudation, surface of a wound incrustation is promoted to improve the healing rate of the surface of a wound to infection control.Herbal mixture of the present invention can obviously accelerate the immediate union of wound and later stage healing, the healing rate of wound are substantially better than jingwanhong soft plaster ointment；The herbal mixture easily absorbs, and ensures the surface of a wound drying after use, reduces the secretion of the surface of a wound, reduce the generation of infection conditions；In wound early stage, it is capable of the content of quick regulating cell growth factor, promotes the healing of wound.
Description
Technical field
The present invention relates to skin wound treatment fields, more particularly to a kind of herbal mixture and its preparation method and application.
Background technology
Skin is human body area maximum and the easy organ by wound, is mainly made of epidermis, corium, subcutaneous tissue,
Due to the vulnerability of skin, clinically the case of skin trauma is relatively common.Antiphlogistic, analgesic are commonly used in skin wound treatment
The infection controls such as medicine and antibiotic and promotion skin healing.But a large amount of uses of antibiotic easy to produce drug resistance, lead to wound
Skin wounds restore slack-off, wound persistently has no that situations such as red and swollen, exudation is with infection occur in healing or even wound.Therefore, such as
Under the premise of where not having to after wound or using hormone antiphlogistic and antibiotic less, by the control of patient's infectious age to minimum,
Improving healing rate makes patient skin site of injury preferably heal, this is the research contents of numerous domestic and foreign scholars' concerns.For
The understanding of skin trauma, the principle that Chinese veterinarian treats skin wound：1. the promoting flow of qi and blood circulation pushes away Chen Zhixin；2. eliminating dampness receives purulence, removes purulence
Myogenic；3. sour and astringent closing in, promoting granulation and wound healing, main principle is to reach myogenic by Eradicates corruption.Full mistake of the external application Chinese medicine in wound repair
The effect of promotion is repaired, main function are all played in journey：1. improving the blood circulation of local woanded surface tissue, enhance local immunity
Function；2. anti-inflammatory, antibacterial, detumescence, analgesic；3. promoting cell Proliferation, accelerates granulation tissue and generate；4. promoting different times cell
The expression of the factor, chemotactic factor (CF).
Currently, the burn and scald for skin have more Chinese medicine, but gone back at present for mechanicalness wound
There is no good Chinese medicine.
Invention content
The purpose of the present invention is to provide a kind of herbal mixtures.It is played between four taste Chinese medicines in herbal mixture of the present invention
Mutual synergistic function accelerates surface of a wound blood coagulation, antiphlogistic antibacterial, reduces surface of a wound exudation, promotes surface of a wound incrustation, to Perceived control
Dye, improves the healing rate of the surface of a wound.
Another object of the present invention is to provide the preparation methods of the herbal mixture.
It is still another object of the present invention to provide application of the herbal mixture in treating skin trauma.
The above-mentioned purpose of the present invention is achieved by the following technical programs：
A kind of herbal mixture, the herbal mixture are made of radix scutellariae, the coptis, the bletilla striata and borneol, and mass ratio is 2~20 ︰ 1
1~10 ︰ 0.1~5 of~15 ︰.
The coptis has the effect of heat-clearing and damp-drying drug, purging intense heat and detonicating, but also the antibacterial action with wide spectrum.
Radix scutellariae has heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis, tocolysis and other effects.Cure mainly warm heat disease, the infection of the upper respiratory tract, lung heat
The diseases such as cough, neonatal jaundice caused by dampness-heat, pneumonia, dysentery, hemoptysis, hot eyes, fetal irritability, hypertension, carbuncle swells furuncle sore.Radix scutellariae also has preferable
Broad-spectrum antibacterial action, and be not easy to develop immunity to drugs.
The bletilla striata has promoting blood circulation and hemostasis effect, and haemostatic effect is fast, safe.Also have simultaneously and promotes keratinocyte
Migration acts on, and has great influence to the immediate union of skin trauma.
Borneol can be used for closing card coma, be used for red eye, swell pain, and larynx numbness aphtha is used for sore swell and ache curative, is not held back after bursting, simultaneously
Also has the function of anti-inflammatory, antibacterial.
Preferably, the mass ratio of radix scutellariae in the herbal mixture, the coptis, the bletilla striata and borneol is 4~10 ︰, 2~8 ︰, 1~7 ︰
0.1~1.
Preferably, the mass ratio of radix scutellariae in the herbal mixture, the coptis, the bletilla striata and borneol is 6 ︰, 4 ︰, 3 ︰ 0.13.
Above-mentioned herbal mixture is prepared by the following method：
S1. radix scutellariae and the coptis are first polished into powder respectively, then the ethyl alcohol for being 95% or more with mass fraction impregnates Huang respectively
A kind of reed mentioned in ancient books and the coptis obtain radix scutellariae ethanol extract and coptis ethanol extract after concentrated extracting solution；The bletilla striata is concentrated to give after adding boiling to boil
To bletilla striata water extract；
S2. first the radix scutellariae ethanol extract of formula ratio and coptis ethanol extract are diluted with water and are sieved respectively, be sieved
Solution and bletilla striata water extract, borneol Hybrid Heating, up to compound Chinese medicine extract after concentration.
Preferably, it is 95% to impregnate radix scutellariae and its mass fraction of the ethyl alcohol of the coptis；The thickening temperature of ethanol extract is 65
~70 DEG C.
Preferably, 120 mesh sieve is crossed after radix scutellariae ethanol extract and coptis ethanol extract are diluted with water.
Preferably, the time that radix scutellariae and the coptis are impregnated in ethanol is for 24 hours.
Preferably, 5~10min of constantly boiling after the bletilla striata adds boiling to boil；The dosage for boiling preceding water is 10 times of bletilla striata weight；
The extracting solution finally obtained is identical as bletilla striata weight.
Application of the above-mentioned herbal mixture in preparing treatment skin trauma drug is also within the scope of the present invention.
Preferably, the skin trauma is mechanicalness wound.
Preferably, the skin trauma is skin cut wound.
Preferably, the drug is external used medicine；It is highly preferred that being spray.
Compared with prior art, the invention has the advantages that：
Herbal mixture of the present invention uses four taste Chinese medicines, plays mutual synergistic function between four taste Chinese medicines, adds
Fast surface of a wound blood coagulation, reduces surface of a wound exudation at antiphlogistic antibacterial, promotes surface of a wound incrustation, to infection control, improves the healing speed of the surface of a wound
Degree.Herbal mixture of the present invention can obviously accelerate immediate union and the later stage healing of wound, apparent to the healing state of wound
Better than jingwanhong soft plaster ointment, and healing rate is also significantly better than jingwanhong soft plaster ointment；The herbal mixture easily absorbs, and ensures wound after external application
Face is dried, and is reduced wound secretion, is reduced the generation of infection conditions；Wound early stage, can quickly regulating cell growth because
The content of son, promotes the healing of wound.
Description of the drawings
Fig. 1 is the case where each group tests wound healing after rabbit skin holostrome cut wound model is treated.
Fig. 2 is that each group tests the 28th day skin histology healing state of rabbit skin holostrome cut wound model (HE is dyed, 400 times).
Specific implementation mode
The present invention is made with reference to specific embodiment and further being elaborated, the embodiment is served only for explaining this
Invention, is not intended to limit the scope of the present invention.Test method used in following embodiments is normal unless otherwise specified
Rule method；Used material, reagent etc., unless otherwise specified, for the reagent and material commercially obtained.
Embodiment 1
A kind of herbal mixture, used raw material have：Radix scutellariae 150g, coptis 100g, bletilla striata 75g, borneol 3.25g.
The preparation process of herbal mixture is as follows：
1. radix scutellariae 150g beats powder, 95% ethyl alcohol 1500mL is added and impregnates 24 hours；
2. coptis 100g beats powder, 95% ethyl alcohol 1000mL is added and impregnates 24 hours；
3. borneol 3.25g, it is spare to be ground into powdery；
4. by 24 hours radix scutellariaes and rhizoma extracting liquid have been impregnated in 95% ethyl alcohol, respectively it is put into Rotary Evaporators and heats
(65-70 degrees Celsius) concentration 30-50min is at dry extract；
5. the dilution of 150mL pure water is added in radix scutellariae dry extract, it is spare to cross 120 mesh sieve；
6. the dilution of 100mL pure water is added in coptis dry extract, it is spare to cross 120 mesh sieve；
7. bletilla striata 75g is added 750mL pure water and boils after boiling 5-10min is concentrated into 75mL, cooling is spare；
8. by 5., 6., 7. in reserve liquid heating mixing after be added 3., stirring is boiled up to herbal mixture.
9. finished product is poured into spray bottle, places 4-8 degrees Celsius of refrigerator and save backup.
Embodiment 2
A kind of compound Chinese medicine extract, used raw material have：Radix scutellariae 250g, coptis 150g, bletilla striata 100g, borneol 20g.
The preparation process of compound Chinese medicine extract is the same as embodiment 1.
Embodiment 3
A kind of compound Chinese medicine extract, used raw material have：Radix scutellariae 400g, coptis 350g, bletilla striata 200g, borneol 50g.
The preparation process of compound Chinese medicine extract is the same as embodiment 1.
Embodiment 4
A kind of compound Chinese medicine extract, used raw material have：Radix scutellariae 100g, coptis 130g, bletilla striata 40g, borneol 8g.
The preparation process of compound Chinese medicine extract is the same as embodiment 1.
Embodiment 5
A kind of compound Chinese medicine extract, used raw material have：Radix scutellariae 200g, coptis 125g, bletilla striata 80g, borneol 4g.
The preparation process of compound Chinese medicine extract is the same as embodiment 1.
Embodiment 6
By taking herbal mixture prepared by embodiment 1 as an example, its therapeutic effect for skin trauma is tested.
1, experiment packet
After selecting healthy adult rabbit 24, adaptability to raise 1 week, it is randomly divided into 4 groups, every group 6 (half male and half female), the 1st
Group herbal mixture group, the 2nd group is jingwanhong soft plaster group, and the 3rd group is model control group, and the 4th group is blank group.
Jingwanhong soft plaster ointment mainly by radix ampelopsis, the root of Dahurain angelica, Chinese lobelia, borneol, rhizoma atractylodis, radix paeoniae rubra, Rhizoma Chuanxiong, pangolin, rheum officinale, when
Return, glutinous rehmannia, garden burnet, safflower, Radix picrorrhizae, the sophora bud, Cortex Phellodendri, the coptis, radix scutellariae, honeysuckle, kuh-seng, myrrh, semen momordicae, pawpaw, breast
Perfume, peach kernel, ground bettle, dark plum, Chinese gall, dragon's blood, carbonized hair, pappy shell, cape jasmine, Asian puccoon, palm, sesame oil and beeswax etc. more than 30
Compound ointment formulation made of taste Chinese medicine has heat clearing and blood circulation promoting, eliminating stasis to subdue swelling, removing necrosis, promoting granulation and convergence analgesic and other effects.By its
When with skin wound, the excipient such as sesame oil and beeswax are not easy to absorb in skin wound, therefore skin wound is more to moisten
Environment, the infection and secretion for being easy to cause skin wound increase, and then leads to surface of a wound skin delayed healing.
1 experiment packet of table and disposition
| Group | Quantity (only) | Drug |
| 1st group | 6 | Herbal mixture |
| 2nd group | 6 | Jingwanhong soft plaster ointment |
| 3rd group | 6 | Physiological saline |
| 4th group | 6 | Nothing |
2, the preparation of skin holostrome cut wound model
It weighs after first each group experimental rabbit is marked, the back rabbit hair is shaved off with razor, one is drawn on rabbit back glabrous skin
Rabbit is put into homemade closed small carton by the circle of diameter 5cm, and a small hole is stayed to lead to anesthesia tube, and breathing anesthesia tube is put
Enter hole, the oxygen concentration of anesthetic gas machine is controlled between 1-1.5, anesthetic concentration controls between 3-4, maintains 2-
5min rabbits can enter depth fiber crops state.By rabbit prostrate, skin holostrome cut wound model is prepared with tissue shear, it is deep to arrive fascia layer, wound
Hinder area (see Fig. 1).After hemostasis by compression, rabbit is put on into No. 6 Elizabethan's circles and puts back to cage.
3, therapeutic process
1st group is done physiological saline cleaning daily and then spray self-control herbal mixture after skin holostrome trauma model, and the 2nd group is done skin
Smear jingwanhong soft plaster ointment after skin holostrome trauma model after the cleaning of daily physiological saline, the 3rd group do it is every after skin holostrome trauma model
It smears physiological saline after being cleaned with physiological saline, the 4th group is not done skin trauma modeling, is normal assays rabbit, normal to raise.Examination
Test rabbit free water in the phase, feeding.
4, result
(1) skin healing situation
From modeling the 1st day~the 28th day, the healing state of each group experimental rabbit wound, obtained the result is shown in Figure 1 are observed.Observation
The healing state of wound in Fig. 1, since skin cut wound the 3rd day, the 1st group of the surface of a wound initially formed crust, the 2nd group and the 3rd group
Skin no significant difference.From the 7th day to the 14th day, the 2nd group of surface of a wound edge starts zooming out, and the 1st group of visible crust of the surface of a wound thickens,
Crust edge is stiff, and edge is free with skin, and surface of a wound area and the 2nd group of no significant difference are substantially reduced than the 3rd group.3 groups of surface of a wound
Skin healing speed is：1st group>2nd group>3rd group.
From the 14th day to 28 days, the 1st group of surface of a wound skin healing speed is accelerated, and 3 groups of surface of a wound skin healing speed are：1st group>
2nd group>3rd group.
Wound surface oozes out situation in observation chart 1, and the 2nd group of surface of a wound exudation is apparent, on the wound middle and later periods rabbit skin surface of a wound
It can be seen that a large amount of white secretion, the 3rd group had water sample exudation, the 1st group of experiment rabbit back surface of a wound to be initially formed from the 3rd day at the 21st day
Crust, subsequent decrustation healing, the surface of a wound are dried without exudation.Degree are oozed out on 3 groups of surface of a wound surfaces：1st group>3rd group>2nd group.
It infects in necrology after the treatment, the 3rd group of experimental rabbit the 5th day, the 8th day and the 9th day each dead one after modeling,
Dissect result is confirmed death reason as wound infection, and dead infection is 50%.2nd group of experimental rabbit the 8th day dead one, dissect
As a result it is wound infection, infectious age 16.67%.1st group of experimental rabbit is without the phenomena of mortality, infectious age 0%.3 groups
Testing wound infection death condition is：3rd group>2nd group>1st group.
From whole observation interpretation of result, healing rate of the 1st group of skin holostrome cut wound in early stage and later stage is all substantially better than
2nd group and the 3rd group, and the 1st group of the surface of a wound is dry environment without exudate.
(2) hematological indices detection and result
Post-traumatic 3rd, 7,14,21,28 day in experimental rabbit, with No. 7 blood taking needles by rabbit auricular vein adopt 1mL whole bloods in
In EDTA anticoagulant tubes, detected for hematological indices.Testing result is shown in Table 2.
Influence of the 24 groups of treatments of table to experiment rabbit skin holostrome cut wound model blood routine
Note：Letter is different on icon indicates significant difference (P<0.05), letter is identical or indicates that difference is not shown without shoulder mark
Write (P>0.05).
Leucocyte (WBC) also entitled immunocyte, it is invaded by phagocytosis to resist pathogenic microorganism, is that body is anti-
One important component of imperial system.Red blood cell (RBC), which has to remove, is immunized circulating complexe, and identification and presentation antigen are made
With, and participate in the immunological regulation of body.Important defense reaction is played on neutrophil cell skin in anti-infective, can cause to feel
Contaminate the inflammatory reaction at position.
As can be seen from Table 2, during testing the treatment of rabbit skin cut wound model, at the 3rd day, the 3rd group of leucocyte
(WBC) it is significantly higher than treatment group and blank group (P<0.05) it, is significantly reduced than the 1st group for the 2nd group, it is not notable with the 4th group of difference.The
3 groups of monocytes are significantly higher than the 1st group, 2 groups and 4 groups, and difference is not notable between other experiment each groups.1st group, 2 groups and 3 groups it
Between red blood cell count(RBC) and hemoglobin difference it is not notable, be below the 4th group.7th day, the 2nd group of leucocyte obviously increased, the
1 group not notable with the 4th group of difference；14th day, the 2nd group of neutrophil cell was significantly higher than other each groups, and the 1st group poor with the 4th group
It is different little.1st group of red blood cell count(RBC), hemoglobin and the 4th group of no significant difference, dramatically increases than the 2nd group and the 4th group.1st
The platelet count of group is dramatically increased than the 2nd group, the 3rd group and the 4th group；28th day, the 1st group of white blood cell count(WBC) and the 4th group of difference were not
Significantly, the 3rd group of leucocyte group obviously increases.
During testing the treatment of rabbit skin cut wound model, inflammation initial stage, the 3rd group of leucocyte (WBC) is significantly higher than
1st group, 2 groups and 4 groups of (P<0.05), illustrate that the 1st group is played antiinflammation with the 2nd group for the treatment of skin trauma.In the inflammation later stage,
2 groups of leucocyte obviously increases, and the 1st group not notable with the 4th group of difference, illustrates herbal mixture in the therapeutic process of skin wound
Anti-inflammatory effects are more more stable than jingwanhong soft plaster ointment anti-inflammatory effects.
(3) blood parameters detection and result
Post-traumatic 3rd, 7,14,21,28 day in rabbit, 3mL whole bloods are adopted in common by rabbit auricular vein with No. 7 blood taking needles
In heparin tube, it is stored at room temperature 30min, waits for that blood sample solidifies completely, 3000 turns/min, centrifugation 5min detaches serum, and is put in -20 DEG C
Refrigerator preserves, and part serum is for measuring blood parameters.Testing result is shown in Table 3.
The influence of 34 groups of Experiment on therapy rabbit skin holostrome cut wound model blood parameters of table
Note：Letter is different on icon indicates significant difference (P<0.05), letter is identical or indicates that difference is not shown without shoulder mark
Write (P>0.05).
ALT and AST in liver, heart tissue, both normal conditions under the flat barrier action of cell membrane not
Easily effusion, the content in serum is extremely low, therefore ALT and AST are used as detecting the whether normal sensitive indicator of liver function,
ALB is to maintaining blood volume, osmotic pressure and circumfusion to play an important role, and GLO has immunization, and body is by exotic antigen
The immunoglobulin of different type and quantity can be generated when invasion according to the type of invasion antigen, TP is made of ALB and GLO,
It is the one of the important signs that of protein metabolism level.
As shown in table 3, in blood parameters detection, at the 3rd day, the 1st group, the 3rd group and the 4th group of glutamic-oxalacetic transaminease
(AST) it is significantly higher than the 2nd group.1st albumin (ALB) and the 4th group of difference is not notable.3rd group of total protein (TP) is apparent high
Difference is not notable between the 1st group, 2 groups and 4 groups, this three groups.2nd group of lactic dehydrogenase (LDH) is substantially less than the 1st group, 3 groups
With 4 groups, this three groups between difference it is not notable；At the 7th day, the 1st group AST, ALB are substantially less than the 2nd group, 3 groups and 4 groups.1st group
TP and the 2nd group of difference it is not notable, with the 4th group and model group significant difference；At the 14th day, the 1st group, 2 groups and 3 groups of AST,
ALB is substantially less than the 4th group.2nd GIU is apparently higher than the 1st group, 3 groups and 4 groups；At the 21st day, the 1st group, 2 groups and 3 groups of TP is aobvious
It writes higher than the 4th group, and difference unobvious between three groups.At the 28th day, the 1st group, 2 groups and 4 groups ALB, TP are significantly higher than the 3rd
Group.
Test result shows in blood parameters detection, at inflammation initial stage, the 1st group, 3 groups and 4 groups of glutamic-oxalacetic transaminease
(AST) it is significantly higher than the 2nd group.1st group of albumin (ALB) and the 4th group of difference is not notable.3rd group of total protein (TP) is apparent
Higher than the 1st group, 2 groups and 4 groups, difference is not notable between this three groups.Illustrate herbal mixture in the treatment of rabbit skin incised wound, liver, the heart
Function is substantially at normal value, as a result relatively stable.In the inflammation later stage, the 1st group, 2 groups and 4 groups ALB, TP are significantly higher than the 3rd
Group.1st group and 2 groups can increase TP, ALB content in skin wound experimental rabbit peripheral blood serum.
(4) cell factor Indexs measure and result
By isolated sera components for detecting cell factor (TNF-a, TGF-BI, NGF, PDGF, VEGF, FGF-
2) it, is measured using sandwich method enzyme-linked immunosorbent assay (ELISA), kit is purchased from the enzyme-linked biological Co., Ltd in Shanghai.Detection
It the results are shown in Table 4.
The influence of 44 groups of Experiment on therapy rabbit skin holostrome cut wound model blood cell levels of factor of table
Note：Letter is different on icon indicates significant difference (P<0.05), letter is identical or indicates that difference is not shown without shoulder mark
Write (P>0.05).
Fibroblast growth factor (FGF-2) can promote the migration of endothelial cell and the proliferation of smooth muscle cell, can
Promote neovascularization, repair the endothelial cell of damage, chronic wounds healing, possible machine can be significantly speeded up
System is related to the deficiency or regulation and control endogenous bFGF activity that Exogenous bFGF can supplement endogenous bFGF.Nerve growth factor
(NGF) a kind of nerve growth regulatory factor with neurotrophic and rush enation double biological function, it is right
Development, differentiation, growth, regeneration and the expression of functional characteristic of maincenter and peripheral neurons all have important regulating and controlling effect.People
The nerve growth factor that class skin capillary endothelial cell generates is related with processes of wound repair.
As shown in table 4, during testing the treatment of rabbit skin cut wound model, at the 14th day, the 1st group of FGF-2 and the 4th
Group no significant difference, substantially less than the 2nd group and the 3rd group；At the 28th day, the 3rd group NGF, TNF-a are significantly higher than the 1st group, 2 groups
With 3 groups.
During testing the treatment of rabbit skin cut wound model, at the 14th day, the 1st group of FGF-2 is with the 4th group without obviously poor
It is different, substantially less than the 2nd group and 3 groups.Illustrate the 1st group of fibroblast growth factor in union of wounded skin regulation and control degree and normal group
It is no different, the 2nd group and 3 groups still in regulation and control.At the 28th day, the 3rd group NGF, TNF-a are significantly higher than the 1st group, 2 groups and 4
Group.It these results suggest that the wound healing later stage, the 1st group is cut with 2 groups of neurotrophic factor with neoplasm growth factor pair skin
The regulation and control for hindering experimental rabbit are basicly stable, and the 3rd group also in regulation and control state.
(5) histology and result
The 28th day after testing rabbit skin holostrome cut wound model trauma care, every group was put to death an experimental rabbit at random,
Wound tissue trauma center point takes 1cm2Skin histology is placed in 10% neutral formalin and fixes, paraffin embedding, HE dyeing, observation
Organization healing situation.Testing result is as shown in Figure 2.
As shown in Figure 2, in histotomy observation in the 28th day, the 3rd group of skin wound tissue visible vessels generate, skin
Organization healing situation is in granulation tissue and generates and the vascularization phase；At the visible wound repair cell of 2nd group of skin wound tissue
In in differentiation state, a part is divided into close epithelial tissue, and a part is divided into loose subcutaneous tissue；1st group of skin
Wound tissue wound repair cell has broken up completion, the visible apparent tissue reconstruction state of skin wound.
Above the experiment results show that herbal mixture of the present invention for cutting skin wound entirely has the function of healing acceleration,
Getting up early healing and the later stage healing that wound can obviously be accelerated, jingwanhong soft plaster ointment is substantially better than to the healing state of wound；In wound
In early days, it is capable of the content of quick regulating cell growth factor, promotes the healing of wound.
In addition, experimental study is shown, radix scutellariae, the coptis, four kinds of components of the bletilla striata and borneol proportioning for Chinese medicine compound prescription skin
Skin wound repair effect has significant impact, when the mass ratio of radix scutellariae, the coptis, the bletilla striata and borneol be 2~20 ︰, 1~15 ︰ 1~
When 10 0.1~5 ︰, preferable curative effect can be achieved；And it is better when mass ratio is 1~7 0.1~1 ︰ 2~8 ︰ 4~10 ︰；
Four best mass ratioes are 6 ︰, 4 ︰, 3 ︰ 0.13.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than is protected to the present invention
The limitation of shield range can also be made on the basis of above description and thinking for those of ordinary skill in the art
Other various forms of variations or variation, there is no necessity and possibility to exhaust all the enbodiments.It is all the present invention
All any modification, equivalent and improvement etc., should be included in the protection of the claims in the present invention made by within spirit and principle
Within the scope of.
Claims (10)
1. a kind of herbal mixture, which is characterized in that the herbal mixture is made of radix scutellariae, the coptis, the bletilla striata and borneol, mass ratio
For 2~20 ︰, 1~15 ︰, 1~10 ︰ 0.1~5.
2. according to claim 1 herbal mixture, which is characterized in that radix scutellariae in the herbal mixture, the coptis, the bletilla striata and borneol matter
Amount is than being 4~10 ︰, 2~8 ︰, 1~7 ︰ 0.1~1.
3. according to claim 1 herbal mixture, which is characterized in that radix scutellariae in the herbal mixture, the coptis, the bletilla striata and borneol matter
Amount is than being 6 ︰, 4 ︰, 3 ︰ 0.13.
4. according to any herbal mixture of claims 1 to 3, which is characterized in that be prepared by the following method：
S1. radix scutellariae and the coptis are first polished into powder respectively, then the ethyl alcohol for being 95% or more with mass fraction impregnate respectively radix scutellariae and
The coptis obtains radix scutellariae ethanol extract and coptis ethanol extract after concentrated extracting solution；The bletilla striata is concentrated to give white after adding boiling to boil
Splendid achnatherum water extract；
S2. first the radix scutellariae ethanol extract of formula ratio and coptis ethanol extract are diluted with water and are sieved respectively, be sieved solution
With bletilla striata water extract, borneol Hybrid Heating, up to compound Chinese medicine extract after concentration.
5. herbal mixture according to claim 4, which is characterized in that impregnate radix scutellariae and the ethyl alcohol of the coptis its mass fraction is
95%；The thickening temperature of ethanol extract is 65~70 DEG C.
6. herbal mixture according to claim 4, which is characterized in that radix scutellariae ethanol extract and coptis ethanol extract add water
120 mesh sieve is crossed after dilution.
7. herbal mixture according to claim 4, which is characterized in that the time that radix scutellariae and the coptis are impregnated in ethanol is for 24 hours.
8. herbal mixture described in claims 1 to 3 is preparing the application in treating skin trauma drug.
9. applying according to claim 8, which is characterized in that the skin trauma is mechanicalness wound.
10. applying according to claim 8, which is characterized in that the skin trauma is skin cut wound.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810253967.9A CN108272959A (en) | 2018-03-26 | 2018-03-26 | A kind of herbal mixture and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810253967.9A CN108272959A (en) | 2018-03-26 | 2018-03-26 | A kind of herbal mixture and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108272959A true CN108272959A (en) | 2018-07-13 |
Family
ID=62810458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810253967.9A Pending CN108272959A (en) | 2018-03-26 | 2018-03-26 | A kind of herbal mixture and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108272959A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110624054A (en) * | 2019-11-05 | 2019-12-31 | 中国人民解放军东部战区空军医院 | Traditional Chinese medicine compound hydrogel for treating skin wound healing and preparation method and application thereof |
| CN111601611A (en) * | 2018-11-27 | 2020-08-28 | 得道健康管理有限公司 | Traditional Chinese medicine composition and preparation process and application thereof |
| CN114533836A (en) * | 2021-12-29 | 2022-05-27 | 朗肽生物制药股份有限公司 | Preparation without affecting bFGF activity and preparation method and application thereof |
| CN114796362A (en) * | 2022-05-13 | 2022-07-29 | 深圳市容宜生物工程有限公司 | Biological preparation for rust scratch repair cells |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101879283A (en) * | 2010-06-13 | 2010-11-10 | 中国人民解放军第三军医大学 | Novel membrane spraying preparation for skin trauma and preparation method |
| CN104524219A (en) * | 2015-01-08 | 2015-04-22 | 遵义医学院 | Giant salamander grease compound burn medicine and preparation method thereof |
| CN104840749A (en) * | 2015-05-20 | 2015-08-19 | 泸州康德缘医药科技开发有限公司 | Externally used heavy-metal-free ointment for treating skin wound and preparation method |
-
2018
- 2018-03-26 CN CN201810253967.9A patent/CN108272959A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101879283A (en) * | 2010-06-13 | 2010-11-10 | 中国人民解放军第三军医大学 | Novel membrane spraying preparation for skin trauma and preparation method |
| CN104524219A (en) * | 2015-01-08 | 2015-04-22 | 遵义医学院 | Giant salamander grease compound burn medicine and preparation method thereof |
| CN104840749A (en) * | 2015-05-20 | 2015-08-19 | 泸州康德缘医药科技开发有限公司 | Externally used heavy-metal-free ointment for treating skin wound and preparation method |
Non-Patent Citations (2)
| Title |
|---|
| 万大群等: "白及的成分、药理作用和临床应用研究进展", 《中国药业》 * |
| 卢文胜等: "烧伤涂膜剂的研制", 《海峡药学》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111601611A (en) * | 2018-11-27 | 2020-08-28 | 得道健康管理有限公司 | Traditional Chinese medicine composition and preparation process and application thereof |
| CN110624054A (en) * | 2019-11-05 | 2019-12-31 | 中国人民解放军东部战区空军医院 | Traditional Chinese medicine compound hydrogel for treating skin wound healing and preparation method and application thereof |
| CN114533836A (en) * | 2021-12-29 | 2022-05-27 | 朗肽生物制药股份有限公司 | Preparation without affecting bFGF activity and preparation method and application thereof |
| CN114533836B (en) * | 2021-12-29 | 2023-01-31 | 朗肽生物制药股份有限公司 | Preparation without affecting bFGF activity and preparation method and application thereof |
| CN114796362A (en) * | 2022-05-13 | 2022-07-29 | 深圳市容宜生物工程有限公司 | Biological preparation for rust scratch repair cells |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108272959A (en) | A kind of herbal mixture and its preparation method and application | |
| CN102091203B (en) | External traditional Chinese medicine preparation for treating chronic wounds and preparation method thereof | |
| CN102048894B (en) | Traditional Chinese medicine composition for treating burn and preparation method thereof | |
| CN101879283B (en) | Novel membrane spraying preparation for skin trauma and preparation method | |
| CN103656372A (en) | Diabetic foot ointment | |
| CN101584810B (en) | Medicine composition for treating gastritis and peptic ulcer and preparation method thereof | |
| CN102085315A (en) | Traditional Chinese medicine composition for treating acute and chronic osteomyelitis | |
| CN103933547A (en) | Application of recombinant lucid ganoderma immune regulation protein in preparing medicine for treating skin tissue senility and wound | |
| CN104825941B (en) | A kind of herbal composite and preparation method thereof for treating tinea pedis | |
| CN101327231B (en) | Chinese medicinal composition for treating psoriasis | |
| CN101559178A (en) | Medicated thread for curing acute nephritis, chronic nephritis and chronic renal failure and application thereof | |
| CN105168941A (en) | Traditional Chinese medicine preparation for promoting cesarean wound healing and preparation method therefor | |
| CN103239665B (en) | Traditional Chinese medicine composition for treating sepsis | |
| CN105287726A (en) | Traditional Chinese medicine preparation applied to Yongquan acupoint for preventing and treating repeated attack of cough and asthma of chronic pulmonary system diseases | |
| CN101181431B (en) | Coptis cream for taking poison and growing muscle due to empyrosis as well as preparation method thereof | |
| CN103169846A (en) | Skin burn-scald spraying agent and preparation method thereof | |
| Miao et al. | Effect of topical application of two Polygala tenuifolia species decoction on guinea pig skin | |
| CN108815268A (en) | A kind of external application promotees the Chinese medicine ointment formulation and preparation method thereof of union of wounded skin | |
| CN109125492A (en) | A kind of removing toxic substances of snakebite dissipates and preparation method thereof | |
| CN103272122A (en) | Traditional Chinese medicinal composition for treating Chronic aplastic anemia | |
| CN102824506A (en) | Traditional Chinese medicine preparation for treating aplastic anemia | |
| CN102526382A (en) | Plaster for relieving swelling and pain | |
| CN108077907A (en) | Biological reinforced cell nutritious element is preparing the application in treating burn and scald dietetic food | |
| CN101062131A (en) | Chinese traditional medicine took orally by patients before induced abortion operation for preventing the postoperative complications | |
| Jiang et al. | Traditional Medicine and Tissue Repair and Regeneration |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180713 |
|
| RJ01 | Rejection of invention patent application after publication |