CN108269032A - A kind of sample operations method and device in nitrogen ultra low temperature sample preservation library - Google Patents
A kind of sample operations method and device in nitrogen ultra low temperature sample preservation library Download PDFInfo
- Publication number
- CN108269032A CN108269032A CN201611254481.4A CN201611254481A CN108269032A CN 108269032 A CN108269032 A CN 108269032A CN 201611254481 A CN201611254481 A CN 201611254481A CN 108269032 A CN108269032 A CN 108269032A
- Authority
- CN
- China
- Prior art keywords
- sample
- storage
- preservation
- storing box
- freezing storing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 218
- 238000004321 preservation Methods 0.000 title claims abstract description 156
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 109
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000007710 freezing Methods 0.000 claims abstract description 116
- 230000008014 freezing Effects 0.000 claims abstract description 116
- 239000007788 liquid Substances 0.000 claims abstract description 76
- 241000894007 species Species 0.000 claims description 18
- 238000005070 sampling Methods 0.000 claims description 13
- 230000003071 parasitic effect Effects 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 230000007613 environmental effect Effects 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 235000015110 jellies Nutrition 0.000 claims 1
- 239000008274 jelly Substances 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000012545 processing Methods 0.000 abstract description 4
- 239000000523 sample Substances 0.000 description 287
- 230000000875 corresponding effect Effects 0.000 description 6
- 238000001514 detection method Methods 0.000 description 3
- 238000012800 visualization Methods 0.000 description 2
- 230000004308 accommodation Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
- G06Q10/00—Administration; Management
- G06Q10/08—Logistics, e.g. warehousing, loading or distribution; Inventory or stock management
- G06Q10/087—Inventory or stock management, e.g. order filling, procurement or balancing against orders
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Business, Economics & Management (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Theoretical Computer Science (AREA)
- Economics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Quality & Reliability (AREA)
- Warehouses Or Storage Devices (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Accounting & Taxation (AREA)
- Finance (AREA)
- Development Economics (AREA)
- Virology (AREA)
- Entrepreneurship & Innovation (AREA)
- Human Resources & Organizations (AREA)
- Marketing (AREA)
- Operations Research (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Databases & Information Systems (AREA)
- Medicinal Chemistry (AREA)
- General Business, Economics & Management (AREA)
- General Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Bioethics (AREA)
- Biophysics (AREA)
- Tourism & Hospitality (AREA)
- Bioinformatics & Computational Biology (AREA)
- Evolutionary Biology (AREA)
- Medical Informatics (AREA)
Abstract
The applicable sample preservation technology field of the present invention, provides a kind of sample operations method and device in nitrogen ultra low temperature sample preservation library, method includes：Receive the sample information of sample to be put in storage input by user, according to the information of freezing storing box in sample information and liquid nitrogen sample preservation library, generate the sample storage request slip of sample to be put in storage, detect the signature to sample storage request slip, when detecting the pre- default access of meeting, after the signature of sample storage request slip, position to user's output for the freezing storing box of sample to be put in storage distribution, positioning freezing storing box simultaneously takes out freezing storing box, sample to be put in storage is put into freezing storing box, and freezing storing box is put back into liquid nitrogen sample preservation library, locking liquid nitrogen sample preservation library, so as to complete the in-stockroom operation of sample to be put in storage, so as to realize the quick positioning of freezing storing box by obtaining sample deposit position automatically, improve the processing safety of user in Sample preservation, the safety of the warehouse-in efficiency and sample of sample is improved simultaneously.
Description
Technical field
The invention belongs to sample preservation technology field more particularly to a kind of sample operations in nitrogen ultra low temperature sample preservation library
Method and device.
Background technology
In biomolecular experiments and culture presevation field, generally require to carry out quick-frozen and ultralow temperature to original sample material
It preserves, just can guarantee the integrality of its DNA and RNA.For culture presevation field, bacterial activity in nitrogen ultra low temperature theory of storage
It can keep more than a century.The particularly high speed development of high throughput sequencing technologies in recent years promotes the sample that ultralow temperature is needed to store
There is exploding for geometry rank in quantity.Although -80 DEG C of ultra low temperature freezers can also carry out ultralow temperature to sample to a certain extent
It preserves, but it is limited to the influence of electric power and circuit, once occur having a power failure or refrigerator circuit system failure, the safety of sample
It will greatly be threatened.The storage position of liquid nitrogen storage tank in itself does not depend on electric power, and storage temperature is down to -196 DEG C, sample
This safety is more secure.
Nitrogen ultra low temperature sample preservation library is divided into large-scale library and small-sized library.In daily scientific research, frequency of use is highest
For the small-sized library of 4000 sample bits, the small-sized library general structure of 4000 sample bits is four hanging rods, and each hanging rod has 10 layers of sample
Freezing storing box position, each sample freezing storing box have 100 sample bits (10*10 structures).The sample size of the type sample database completely may be used
To meet the daily demand of the project correlated samples dynamic memory of general seminar, and required facility is compared to large-scale library significantly
Lower the requirement, and extend conveniently, electric system can be completely independent of, accomplish unpowered independent operating, routine use into
This is very low.However, the daily management in existing small-sized nitrogen ultra low temperature sample preservation library also relies on paper record or excel substantially
Table class software, the efficiency of management of administrator are low.Sample generally requires sample supplier when being put in storage and provides number of samples first
And title, it checks record by administrative staff, manually chooses the vacancy point in current library, be then manually assigned to each sample, most
The site data provided afterwards by operator according to administrative staff, sample is put in storage.Due to sample database during routine use by
Being analyzed in project testing needs, and often has sample and is removed, and new sample is placed into, so what each sample site was stored
Sample is dynamic change, and it is all continued presence that vacancy point, which is not, yet, the freezing storing box of different hanging rod different layers that may be distributed
It is interior so that vacancy point location is difficult.In addition, for in-stockroom operation person, the proposition of sample hanging rod, which is put into, itself has certain danger
Dangerous (liquid nitrogen temperature is too low, and contact skin can cause to damage), and sample in place in freezing storing box does not also allow to operate
They are exposed to other than liquid nitrogen environment by member for a long time, and the delay of operating time can be to being RNA, DNA etc. to multigelation sensitivity
Sample generate very big influence, and for the strain of preservation, after the recurrent fluctuations of this temperature can substantially reduce
Recovery survival rate.
The sample reserves in large-scale library may be up to hundreds thousand of ranks, and generally large-scale center of a sample or gene pool use, and build
Cost and maintenance cost are very high.The storage in existing large size nitrogen ultra low temperature sample preservation library and outbound operation generally by with
The mode that two-dimensional code scanning and mechanical arm extraction are combined is realized, however, these equipment or system of storage and outbound are easily
Hundreds thousand of prices, the value (tens thousand of members) far beyond nitrogen storage tank in itself, most extensive for the scope of application
The small samples libraries of 4000 sample bits be not appropriate for.
Invention content
The purpose of the present invention is to provide a kind of sample operations method and devices in nitrogen ultra low temperature sample preservation library, it is intended to
It solves that due to the prior art a kind of sample operations side that effectively can be used for small-sized nitrogen ultra low temperature sample preservation library can not be provided
Method leads to the problem of sample safety reduces when sample operations inefficiency, the operation in small-sized nitrogen ultra low temperature sample preservation library.
On the one hand, the present invention provides a kind of sample operations method in nitrogen ultra low temperature sample preservation library, the method packets
Include following step：
Receive the sample information of sample to be put in storage input by user；
According to the information of freezing storing box in the sample information and liquid nitrogen sample preservation library, the sample of sample to be put in storage described in generation
This storage request slip, the sample storage request slip include the sample identification symbol of the sample to be put in storage and wait to be put in storage to be described
The position of the freezing storing box of sample distribution；
Detect to the sample storage request slip signature, when detect it is pre- meet default access, the sample is entered
After the signature of library request slip, the position to user output for the freezing storing box of the sample distribution to be put in storage；
It is positioned in the liquid nitrogen sample preservation library according to the position of the output and is frozen described in the freezing storing box and taking-up
The sample to be put in storage is put into the freezing storing box by box, and the freezing storing box is put back into the liquid nitrogen sample preservation library,
Liquid nitrogen sample preservation library described in locking, so as to complete the in-stockroom operation of the sample to be put in storage.
On the other hand, the present invention provides a kind of sample operations device in nitrogen ultra low temperature sample preservation library, described devices
Including：
Information receiving unit, for receiving the sample information of sample to be put in storage input by user；
Storage bill generation unit, it is raw for the information according to freezing storing box in the sample information and liquid nitrogen sample preservation library
Sample into the sample to be put in storage is put in storage request slip, and the sample storage request slip includes the sample mark of the sample to be put in storage
Know the position of symbol and the freezing storing box for the sample distribution to be put in storage；
First position output unit, for detect to the sample storage request slip signature, when detect it is pre- meet it is pre-
If permission, to the signature of sample storage request slip after, the institute that is distributed to user output for the sample to be put in storage
State the position of freezing storing box；And
Go out library unit, the freezing storing box is positioned simultaneously in the liquid nitrogen sample preservation library for the position according to the output
The freezing storing box is taken out, the sample to be put in storage is put into the freezing storing box, and the freezing storing box is put back into the liquid nitrogen
In sample preservation library, liquid nitrogen sample preservation library described in locking, so as to complete the in-stockroom operation of the sample to be put in storage.
The present invention is input by user after the sample information of storage sample in reception, is protected according to sample information and liquid nitrogen sample
The information of freezing storing box in library is hidden, generates the sample storage request slip of sample to be put in storage, further detection is put in storage request slip to sample
Signature, when have detect it is pre- meet default access, to sample storage request slip signature after, just to user output for treat into
The position of the freezing storing box of library sample distribution, so as to ensure that the safety of in-stockroom operation, finally according to the position of output in liquid nitrogen
Freezing storing box is positioned in sample preservation library and takes out freezing storing box, sample to be put in storage is put into freezing storing box, and freezing storing box is put back into
In liquid nitrogen sample preservation library, the in-stockroom operation of sample to be put in storage is completed in locking liquid nitrogen sample preservation library, so as to by obtaining automatically
Sample deposit position realizes the quick positioning of freezing storing box, improves the processing safety of user in Sample preservation, improves simultaneously
The safety of the warehouse-in efficiency and sample of sample.
Description of the drawings
Fig. 1 is the realization flow of the sample operations method in the nitrogen ultra low temperature sample preservation library that the embodiment of the present invention one provides
Figure；
Fig. 2 is the realization flow of the sample operations method in nitrogen ultra low temperature sample preservation library provided by Embodiment 2 of the present invention
Figure；
Fig. 3 is the structural representation of the sample operations device in the nitrogen ultra low temperature sample preservation library that the embodiment of the present invention three provides
Figure；And
Fig. 4 is the preferred structure of the sample operations device in the nitrogen ultra low temperature sample preservation library that the embodiment of the present invention three provides
Schematic diagram.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to the accompanying drawings and embodiments, it is right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
The specific implementation of the present invention is described in detail below in conjunction with specific embodiment：
Embodiment one：
Fig. 1 shows the realization of the sample operations method in the nitrogen ultra low temperature sample preservation library that the embodiment of the present invention one provides
Flow for convenience of description, illustrates only and the relevant part of the embodiment of the present invention.
The embodiment of the present invention is suitable for small-sized nitrogen ultra low temperature sample preservation library, for being carried out to the sample preservation library
Sample operationies on incoming and outgoing inventory, specifically, suitable for having the small-sized nitrogen ultra low temperature sample preservation library of 4000 sample accommodated positions.Make
For illustratively, for example, the nitrogen ultra low temperature sample preservation library can have there are four hanging rod, each hanging rod has 10 layers of sample freezing storing box
Position, each sample freezing storing box has 100 sample bits (10*10 structures), to accommodate 4000 samples.
In step S101, the sample information of sample to be put in storage input by user is received.
In embodiments of the present invention, the sample information of the sample to be put in storage of input can include sampling place name, species name, sample
This position, sample batch, the category-information such as kind of name and/or sample number.
In step s 102, according to the information of freezing storing box in sample information and liquid nitrogen sample preservation library, sample to be put in storage is generated
This sample storage request slip.
In embodiments of the present invention, sample storage request slip includes the sample identification symbol of sample to be put in storage and to wait to be put in storage
The position of the freezing storing box of sample distribution, naturally it is also possible to further comprise sample information, directly acquire in order to subsequent operation or
Understand the essential information of sample.It further, should be according to liquid nitrogen sample before the sample storage request slip for generating sample to be put in storage
The classification of sample is stored in this preservation library, the sample identification symbol for the storage sample sets corresponding identifier code pattern, with
For automatically generating the sample identification of sample to be put in storage symbol.
Preferably, when storing the classification of sample as high/complicated animal or plant sample, sample identification symbol includes sampling
Place name is write a Chinese character in simplified form, species name is write a Chinese character in simplified form, sample site is write a Chinese character in simplified form, individual is numbered, sample batch is numbered and sample number coding.When storage sample
When this classification is low/simple parasitic species sample, sample identification symbol includes sampling, and place name is write a Chinese character in simplified form, species name is write a Chinese character in simplified form, sample
Developmental stage is write a Chinese character in simplified form, host species name is write a Chinese character in simplified form, parasitic site is write a Chinese character in simplified form, host numbers, sample batch is numbered and sample number coding.
When store sample classification be microorganism pure culture sample when, sample identification symbol include sample place name is write a Chinese character in simplified form, category-kind of name is write a Chinese character in simplified form,
Bacterial strain name and repeated sample number coding.When the classification for storing sample is macro genome sample, sample identification symbol includes sampling
Place name writes a Chinese character in simplified form, individual species name/environmental information is write a Chinese character in simplified form, sample site is write a Chinese character in simplified form, individual/pin numbers, sample batch number, sample
Number and macro genome identification code.In this way, the readability of sample identification symbol can be improved, in order to small-sized nitrogen ultra low temperature sample
The operation in this preservation library.
Further, the letter of freezing storing box in had meaning and liquid nitrogen sample preservation library is encoded according to sample identification symbol
Breath, the corresponding freezing storing box for sample to be put in storage distribution.As illustratively, for example, the species name in being accorded with according to sample identification
It is encoded to sample and distributes same hanging rod, for another example, can further be encoded according to sample site and sample distribution is accommodated into position in same layer.
Preferably, sample storage request slip further includes the name or identifier of the user, to ensure the operation of liquid nitrogen sample preservation library
Safety.Wherein, the information of freezing storing box includes the distribution information that sample in liquid nitrogen sample preservation library accommodates position or accommodation space, with
For recording the vacant situation that position is accommodated in liquid nitrogen sample preservation library.
In step s 103, detect to sample storage request slip signature, when detect it is pre- meet default access, to sample
After the signature of this storage request slip, box position is frozen for sample to be put in storage distribution to user's output.
In embodiments of the present invention, in order to ensure the safety of deposit sample and legitimacy, sample storage request slip is necessary
After obtaining the signature with the personnel of certain permission, liquid nitrogen sample preservation library can be just deposited into, so as to ensure liquid nitrogen sample
The safety of sample in preservation library.It specifically, can be to the user of proposition this application when signing to sample storage request slip
Identity is verified that liquid nitrogen sample preservation library whether can be deposited into sample according to sample information after being proved to be successful judges.
It when determining that the sample can be deposited into the liquid nitrogen sample preservation library, signs to this application list, when signature can pass through number
Signature scheme carries out, and can also be carried out by other manner, for example, manual type.
Later, if certain permission, signature to sample storage request slip are detected, to user's output for sample to be put in storage
The position of the freezing storing box of this distribution.Preferably, it at the position for exporting freezing storing box, is exported by voice or image mode to user
The position of freezing storing box for sample to be put in storage distribution so as to carry out operation guide to user with visualization or speech form, is improved
The validity and practicability that user's in-stockroom operation guides.
In step S104, freezing storing box is positioned in liquid nitrogen sample preservation library according to the position of output and takes out freezing storing box,
Sample to be put in storage is put into freezing storing box, and freezing storing box is put back into liquid nitrogen sample preservation library, locking liquid nitrogen sample preservation library,
So as to complete the in-stockroom operation of sample to be put in storage.
The embodiment of the present invention is when sample is put in storage, according to the information of the storage sample received and small-sized liquid nitrogen sample preservation
The information of freezing storing box in library generates the sample storage request slip of sample to be put in storage, and further detection is to sample storage request slip
Signature, when have detect it is pre- meet default access, to the signature of sample storage request slip after, just to user's output to wait to be put in storage
The position of the freezing storing box of sample distribution, so as to ensure that the safety of in-stockroom operation, finally according to the position of output in liquid nitrogen sample
Freezing storing box is positioned in this preservation library and takes out freezing storing box, sample to be put in storage is put into freezing storing box, and freezing storing box is put back into liquid
In nitrogen sample preservation library, the in-stockroom operation of sample to be put in storage is completed in locking liquid nitrogen sample preservation library, so as to by obtaining sample automatically
This deposit position realizes the quick positioning of freezing storing box, improves the processing safety of user in Sample preservation, while improve sample
The safety of this warehouse-in efficiency and sample.
Embodiment two：
Fig. 2 shows the realizations of the sample operations method in nitrogen ultra low temperature sample preservation library provided by Embodiment 2 of the present invention
Flow for convenience of description, illustrates only and the relevant part of the embodiment of the present invention.
The embodiment of the present invention is suitable for small-sized nitrogen ultra low temperature sample preservation library, for being carried out to the sample preservation library
Sample operationies on incoming and outgoing inventory, specifically, suitable for having the small-sized nitrogen ultra low temperature sample preservation library of 4000 sample accommodated positions.Make
For illustratively, for example, the nitrogen ultra low temperature sample preservation library can have there are four hanging rod, each hanging rod has 10 layers of sample freezing storing box
Position, each sample freezing storing box has 100 sample bits (10*10 structures), to accommodate 4000 samples.
In step s 201, the sample information of sample to be put in storage input by user is received.
In step S202, according to the information of freezing storing box in sample information and liquid nitrogen sample preservation library, sample to be put in storage is generated
This sample is put in storage request slip, and sample storage request slip includes the sample identification symbol of sample to be put in storage and divides for sample to be put in storage
The position for the freezing storing box matched.
In step S203, detect to sample storage request slip signature, when detect it is pre- meet default access, to sample
After the signature of this storage request slip, box position is frozen for sample to be put in storage distribution to user's output.
In step S204, freezing storing box is positioned in liquid nitrogen sample preservation library according to the position of output and takes out freezing storing box,
Sample to be put in storage is put into freezing storing box, and freezing storing box is put back into liquid nitrogen sample preservation library, locking liquid nitrogen sample preservation library,
So as to complete the in-stockroom operation of sample to be put in storage.
In embodiments of the present invention, above-mentioned steps can refer to the embodiment of step S101 to S104 in embodiment one,
This is repeated no more.
In step S205, when receiving the request to the preservation sample progress outbound in liquid nitrogen sample preservation library, obtain
It goes bail for and hides the sample identification symbol of sample.
It in step S206, is accorded with according to sample identification, generates the ex-warehouse request note of preservation sample, ex-warehouse request note includes protecting
Hide the position of the sample identification symbol of sample and the freezing storing box of preservation sample.
In embodiments of the present invention, when receive user in liquid nitrogen sample preservation library preservation sample carry out outbound please
When asking, accorded with according to the sample identification of the acquisition request preservation sample.By the code parsing accorded with to the sample identification, can be used
The sample information of outbound sample is asked at family, for example, the sampling place name of outbound sample, species name, sample site, sample batch, category-
The kind information such as name and/or sample number, and then the outbound Shen for asking outbound sample (i.e. preservation sample) is generated according to sample information
It please be single.Wherein, sample ex-warehouse request note may include the sample mark of outbound sample (the preservation sample i.e. in liquid nitrogen sample preservation library)
Know the position of the freezing storing box of symbol and outbound sample, naturally it is also possible to further comprise sample information, in order to which subsequent operation is straight
It obtains and takes or understand sample information.
In step S207, detect the signature to ex-warehouse request note, when detect it is pre- meet default access, to outbound Shen
After signature that please be single, the position of freezing storing box where exporting preservation sample to user.
In embodiments of the present invention, in order to ensure the safety of outbound sample and legitimacy, sample ex-warehouse request note is necessary
It after obtaining the signature with the personnel of certain permission, can just be taken out from liquid nitrogen sample preservation library, so as to ensure that liquid nitrogen sample is protected
Hide the safety of sample and the safety of outbound sample in library.It specifically, can be to proposing when signing to sample ex-warehouse request note
Whether the user identity of this application verified, can be from liquid nitrogen sample preservation library to sample according to sample information after being proved to be successful
Taking-up is judged.It when determining that the sample can take out from the liquid nitrogen sample preservation library, signs, signs to this application list
When can be carried out by digital signature encryption mode, can also be carried out by other manner, for example, manual type.
Later, if detecting certain permission, signature to sample ex-warehouse request note, to user export preservation sample or
The position of freezing storing box where treating outbound sample.Preferably, at the position for exporting freezing storing box, by voice or image mode to
The position of freezing storing box, so as to carry out operation guide to user with visualization or speech form, is improved where family output outbound sample
The validity and practicability that user's outbound operation is guided.
In step S208, the position of freezing storing box positions preservation sample in liquid nitrogen sample preservation library according to where preservation sample
This place freezing storing box simultaneously takes out the freezing storing box, preservation sample is taken out in freezing storing box where from preservation sample, and the freezing storing box is put
It returns in liquid nitrogen sample preservation library, locking liquid nitrogen sample preservation library, so as to complete the outbound of preservation sample operation.
For the embodiment of the present invention when sample goes out, is put in storage, according to the information for going out, being put in storage sample received, generation is corresponding
Sample goes out, is put in storage request slip, further signature of the detection to sample request slip, when have detect it is pre- meet default access, it is right
After the signature of sample request slip, the just position to user's output for corresponding freezing storing box, so as to ensure that out, the peace of in-stockroom operation
Quan Xing so as to realize the quick positioning of freezing storing box by obtaining sample deposit position automatically, is improved during sample goes out, is put in storage
The processing safety of user, while improve the safety of the going out of sample, warehouse-in efficiency and sample.
One of ordinary skill in the art will appreciate that all or part of the steps of the method in the foregoing embodiments are can be with
Relevant hardware is instructed to complete by program, the program can be stored in a computer read/write memory medium,
The storage medium, such as ROM/RAM, disk, CD.
Embodiment three：
Fig. 3 shows the knot of the sample operations device in the nitrogen ultra low temperature sample preservation library that the embodiment of the present invention three provides
Structure, for convenience of description, illustrate only with the relevant part of the embodiment of the present invention, including：
Information receiving unit 31, for receiving the sample information of sample to be put in storage input by user；
Storage bill generation unit 32, for the information according to freezing storing box in sample information and liquid nitrogen sample preservation library, generation
The sample storage request slip of sample to be put in storage, the sample identification symbol of sample storage request slip including sample be put in storage and be treat into
The position of the freezing storing box of library sample distribution；
First position output unit 33, for detect to sample storage request slip signature, when detect it is pre- meet it is default
Permission, to sample storage request slip signature after, to user output for sample be put in storage distribute freezing storing box position；And
Go out library unit 34, position freezing storing box in liquid nitrogen sample preservation library for the position according to output and taking-up freezes
Sample to be put in storage is put into freezing storing box by box, and freezing storing box is put back into liquid nitrogen sample preservation library, locking liquid nitrogen sample preservation
Library, so as to complete the in-stockroom operation of sample to be put in storage.
Preferably, as shown in figure 4, shown in figure the sample operations device in nitrogen ultra low temperature sample preservation library one preferably
Structure, wherein：
Storage bill generation unit 32 includes encoding setting unit 321, for storing sample according in liquid nitrogen sample preservation library
Classification, the sample identification symbol to store sample sets corresponding coding mode, for automatically generating the sample of sample to be put in storage
This identifier.
First position output unit 33 includes position and exports subelement 331, for passing through voice or image mode to described
Position of user's output for the freezing storing box of sample to be put in storage distribution.
The sample operations device in nitrogen ultra low temperature sample preservation library further includes：
Identifier obtaining unit 35 carries out the preservation sample in liquid nitrogen sample preservation library asking for outbound for working as to receive
When asking, the sample identification symbol of preservation sample is obtained；
Outbound list generation unit 36 for being accorded with according to sample identification, generates the ex-warehouse request note of preservation sample, applied ex-warehouse
The position of the freezing storing box of single sample identification symbol for including preservation sample and preservation sample；
Second position output unit 37 for detecting signature to ex-warehouse request note, pre- meets default access when detecting
, to the signature of ex-warehouse request note after, the position of freezing storing box where exporting preservation sample to user；And
Go out library unit 38, the position for the freezing storing box according to where preservation sample positions preservation in liquid nitrogen sample preservation library
Freezing storing box where sample simultaneously takes out the freezing storing box, takes out preservation sample in freezing storing box where from preservation sample, and by the freezing storing box
It is put back into liquid nitrogen sample preservation library, locking liquid nitrogen sample preservation library, so as to complete the outbound of preservation sample operation.
In embodiments of the present invention, each unit of the sample operations device in nitrogen ultra low temperature sample preservation library can be by corresponding
Hardware or software unit realize that each unit can be independent soft and hardware unit, can also be integrated into a soft and hardware unit,
Herein not limiting the present invention.The specific embodiment of each unit can refer to the description of embodiment, and details are not described herein.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (10)
- A kind of 1. sample operations method in nitrogen ultra low temperature sample preservation library, which is characterized in that the method includes following step：Receive the sample information of sample to be put in storage input by user；According to the information of freezing storing box in the sample information and liquid nitrogen sample preservation library, the sample of sample to be put in storage enters described in generation Library request slip, the sample storage request slip include the sample identification symbol of the sample to be put in storage and are the sample to be put in storage The position of the freezing storing box of distribution；Detect to the sample storage request slip signature, when detect it is pre- meet default access, to the sample be put in storage Shen After signature that please be single, the position to user output for the freezing storing box of the sample distribution to be put in storage；The freezing storing box is positioned in the liquid nitrogen sample preservation library according to the position of the output and takes out the freezing storing box, it will The sample to be put in storage is put into the freezing storing box, and the freezing storing box is put back into the liquid nitrogen sample preservation library, locking The liquid nitrogen sample preservation library, so as to complete the in-stockroom operation of the sample to be put in storage.
- 2. the method as described in claim 1, which is characterized in that the step of the sample storage request slip of sample to be put in storage described in generation Before rapid, the method further includes：According to the classification that sample is stored in the liquid nitrogen sample preservation library, the sample identification symbol setting for the storage sample corresponds to Coding mode, accorded with for automatically generating the sample identification of sample be put in storage.
- 3. method as claimed in claim 2, which is characterized in thatWhen the classification of the storage sample is high/complicated animal or plant sample, the sample identification symbol includes sampling ground Name is write a Chinese character in simplified form, species name is write a Chinese character in simplified form, sample site is write a Chinese character in simplified form, individual is numbered, sample batch is numbered and sample number coding；When the classification of the storage sample is low/simple parasitic species sample, the sample identification symbol includes sampling place name It writes a Chinese character in simplified form, species name is write a Chinese character in simplified form, sample developmental stage is write a Chinese character in simplified form, host species name is write a Chinese character in simplified form, parasitic site is write a Chinese character in simplified form, host numbers, sample is criticized Secondary number and sample number coding；When the classification of the storage sample is microorganism pure culture sample, sample identification symbol include sampling place name write a Chinese character in simplified form, Category-kind of name is write a Chinese character in simplified form, bacterial strain name and repeated sample number encode；When the classification of the storage sample is macro genome sample, the sample identification symbol includes sampling place name and writes a Chinese character in simplified form, is individual Species name/environmental information is write a Chinese character in simplified form, sample site is write a Chinese character in simplified form, individual/pin numbers, sample batch number, sample number and macro base Because of a group identification code.
- 4. the method as described in claim 1, which is characterized in that the institute to user output for the sample distribution to be put in storage The step of stating the position of freezing storing box, including：The position of the freezing storing box distributed by voice or image mode to user output for the sample to be put in storage.
- 5. the method as described in claim 1, which is characterized in that the method further includes：When receiving the request to the preservation sample progress outbound in the liquid nitrogen sample preservation library, the preservation sample is obtained Sample identification symbol；It is accorded with according to the sample identification, generates the ex-warehouse request note of the preservation sample, the ex-warehouse request note includes the guarantor Hide the position of the sample identification symbol of sample and the freezing storing box of the preservation sample；Signature to the ex-warehouse request note is detected, pre- meets default access, label to the ex-warehouse request note when detecting After word, the position of freezing storing box where exporting the preservation sample to the user；The position of freezing storing box positions the preservation sample institute in the liquid nitrogen sample preservation library according to where the preservation sample In freezing storing box and the freezing storing box is taken out, the preservation sample is taken out, and this is frozen in freezing storing box where from the preservation sample Box is put back into the liquid nitrogen sample preservation library, liquid nitrogen sample preservation library described in locking, so as to complete going out for the preservation sample Library operates.
- 6. a kind of sample operations device in nitrogen ultra low temperature sample preservation library, which is characterized in that described device includes：Information receiving unit, for receiving the sample information of sample to be put in storage input by user；Storage bill generation unit for the information according to freezing storing box in the sample information and liquid nitrogen sample preservation library, generates institute The sample storage request slip of sample to be put in storage is stated, the sample storage request slip includes the sample identification symbol of the sample to be put in storage And the position of the freezing storing box for the sample distribution to be put in storage；First position output unit for detecting the signature to sample storage request slip, pre- meets default power when detecting Limit, to the signature of sample storage request slip after, the jelly that is distributed to user output for sample be put in storage Deposit the position of box；AndGo out library unit, the freezing storing box is positioned in the liquid nitrogen sample preservation library for the position according to the output and take out The sample to be put in storage is put into the freezing storing box by the freezing storing box, and the freezing storing box is put back into the liquid nitrogen sample In preservation library, liquid nitrogen sample preservation library described in locking, so as to complete the in-stockroom operation of the sample to be put in storage.
- 7. device as claimed in claim 6, which is characterized in that the storage bill generation unit includes：Encoding setting unit, for being the storage sample according to the classification that sample is stored in the liquid nitrogen sample preservation library Sample identification symbol sets corresponding coding mode, for automatically generating the sample identification symbol of the sample to be put in storage.
- 8. device as claimed in claim 7, which is characterized in thatWhen the classification of the storage sample is high/complicated animal or plant sample, the sample identification symbol includes sampling ground Name is write a Chinese character in simplified form, species name is write a Chinese character in simplified form, sample site is write a Chinese character in simplified form, individual is numbered, sample batch is numbered and sample number coding；When the classification of the storage sample is low/simple parasitic species sample, the sample identification symbol includes sampling place name It writes a Chinese character in simplified form, species name is write a Chinese character in simplified form, sample developmental stage is write a Chinese character in simplified form, host species name is write a Chinese character in simplified form, parasitic site is write a Chinese character in simplified form, host numbers, sample is criticized Secondary number and sample number coding；When the classification of the storage sample is microorganism pure culture sample, sample identification symbol include sampling place name write a Chinese character in simplified form, Category-kind of name is write a Chinese character in simplified form, bacterial strain name and repeated sample number encode；When the classification of the storage sample is macro genome sample, the sample identification symbol includes sampling place name and writes a Chinese character in simplified form, is individual Species name/environmental information is write a Chinese character in simplified form, sample site is write a Chinese character in simplified form, individual/pin numbers, sample batch number, sample number and macro base Because of a group identification code.
- 9. device as claimed in claim 6, which is characterized in that the first position output unit includes：Position exports subelement, is that the sample to be put in storage distributes for passing through voice or image mode to user output The position of the freezing storing box.
- 10. device as claimed in claim 6, which is characterized in that described device further includes：Identifier obtaining unit receives the request that outbound is carried out to the preservation sample in the liquid nitrogen sample preservation library for working as When, the sample identification for obtaining the preservation sample accords with；Outbound list generation unit for being accorded with according to the sample identification, generates the ex-warehouse request note of the preservation sample, it is described go out Library request slip includes the position of the sample identification symbol of the preservation sample and the freezing storing box of the preservation sample；Second position output unit, for detecting signature to the ex-warehouse request note, when detect it is pre- meet default access, After the signature of the ex-warehouse request note, the position of freezing storing box where exporting the preservation sample to the user；AndGo out library unit, the position for the freezing storing box according to where the preservation sample positions institute in the liquid nitrogen sample preservation library Freezing storing box where stating preservation sample simultaneously takes out the freezing storing box, and the preservation sample is taken out in freezing storing box where from the preservation sample This, and the freezing storing box is put back into the liquid nitrogen sample preservation library, liquid nitrogen sample preservation library described in locking, it is described so as to complete The outbound operation of preservation sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611254481.4A CN108269032A (en) | 2016-12-30 | 2016-12-30 | A kind of sample operations method and device in nitrogen ultra low temperature sample preservation library |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611254481.4A CN108269032A (en) | 2016-12-30 | 2016-12-30 | A kind of sample operations method and device in nitrogen ultra low temperature sample preservation library |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108269032A true CN108269032A (en) | 2018-07-10 |
Family
ID=62754385
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611254481.4A Pending CN108269032A (en) | 2016-12-30 | 2016-12-30 | A kind of sample operations method and device in nitrogen ultra low temperature sample preservation library |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108269032A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110580599A (en) * | 2019-07-24 | 2019-12-17 | 唐颐(湖州)生物科技有限公司 | Intelligent positioning algorithm and device for biological sample storage space planning |
| CN112550951A (en) * | 2020-12-04 | 2021-03-26 | 上海原能细胞生物低温设备有限公司 | Biological sample storage method and device, computer equipment and storage medium |
| CN114913926A (en) * | 2022-05-31 | 2022-08-16 | 青岛中康原能细胞生物科技有限公司 | Cell warehousing method, device and equipment |
| CN117104748A (en) * | 2023-09-11 | 2023-11-24 | 上海原能细胞生物低温设备有限公司 | Biological sample storage method and automatic storage equipment thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140172154A1 (en) * | 2012-12-17 | 2014-06-19 | Beckman Coulter, Inc. | Method and system using sample processing system and storage units |
| CN105095709A (en) * | 2015-09-09 | 2015-11-25 | 西南大学 | On-line signature identification method and on-line signature identification system |
| CN105469179A (en) * | 2014-09-11 | 2016-04-06 | 深圳华大基因研究院 | Informationalized bio-sample library management system and sample management method |
| CN105956365A (en) * | 2016-04-20 | 2016-09-21 | 广东常青生命科学研究有限公司 | Method and system for managing biological sample |
-
2016
- 2016-12-30 CN CN201611254481.4A patent/CN108269032A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140172154A1 (en) * | 2012-12-17 | 2014-06-19 | Beckman Coulter, Inc. | Method and system using sample processing system and storage units |
| CN105469179A (en) * | 2014-09-11 | 2016-04-06 | 深圳华大基因研究院 | Informationalized bio-sample library management system and sample management method |
| CN105095709A (en) * | 2015-09-09 | 2015-11-25 | 西南大学 | On-line signature identification method and on-line signature identification system |
| CN105956365A (en) * | 2016-04-20 | 2016-09-21 | 广东常青生命科学研究有限公司 | Method and system for managing biological sample |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110580599A (en) * | 2019-07-24 | 2019-12-17 | 唐颐(湖州)生物科技有限公司 | Intelligent positioning algorithm and device for biological sample storage space planning |
| CN112550951A (en) * | 2020-12-04 | 2021-03-26 | 上海原能细胞生物低温设备有限公司 | Biological sample storage method and device, computer equipment and storage medium |
| CN112550951B (en) * | 2020-12-04 | 2023-03-07 | 上海原能细胞生物低温设备有限公司 | Biological sample storage method and device, computer equipment and storage medium |
| CN114913926A (en) * | 2022-05-31 | 2022-08-16 | 青岛中康原能细胞生物科技有限公司 | Cell warehousing method, device and equipment |
| CN114913926B (en) * | 2022-05-31 | 2023-08-22 | 青岛瑞源细胞生物科技开发有限公司 | Cell warehousing method, device and equipment |
| CN117104748A (en) * | 2023-09-11 | 2023-11-24 | 上海原能细胞生物低温设备有限公司 | Biological sample storage method and automatic storage equipment thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108269032A (en) | A kind of sample operations method and device in nitrogen ultra low temperature sample preservation library | |
| WO2016059610A1 (en) | A biomolecule based data storage system | |
| CN111222336B (en) | Method and device for identifying unknown entity | |
| CN114500035B (en) | Data encryption system based on service data sharing cloud platform | |
| CN104899748A (en) | Two-dimensional code and scratch-off code composite identifying method based on identity authentication technology | |
| CN114707166A (en) | Data storage and reading method, device, equipment and storage medium | |
| CN114925853A (en) | Construction method, device, equipment and medium of gradient lifting tree model | |
| CN111177421A (en) | Method and device for generating email historical event axis facing digital human | |
| Alves et al. | Leveraging BERT's Power to Classify TTP from Unstructured Text | |
| US9317499B2 (en) | Optimizing generation of a regular expression | |
| CN102982288B (en) | The encryption of data and the equipment of deciphering and method is performed in portable terminal | |
| CN116070248B (en) | Data processing system and method for ensuring safety of power data | |
| CN108616533B (en) | Sensitive data encryption method and device | |
| KR101521805B1 (en) | Apparatus and method for password searching based on automated combination of dictionary strings | |
| CN101751581B (en) | System for testing radio frequency identification device label data fraud threat | |
| CN105117702A (en) | Vena metacarpea image identifying device | |
| Ruiz‐Gartzia et al. | Recovering the genomes hidden in museum wet collections | |
| CN111435452B (en) | Model training method, device, equipment and medium | |
| Park et al. | Investigation of machine learning algorithms for taxonomic classification of marine metagenomes | |
| CN114338058A (en) | Information processing method, device and storage medium | |
| CN107819882A (en) | A kind of office Internet of Things network control method and system based on RFID | |
| CN113111229A (en) | Regular expression-based method and device for extracting track-to-ground address of alarm receiving and processing text | |
| CN112149140A (en) | Prediction method, device, equipment and storage medium | |
| CN117350460B (en) | Supply chain-based building element informatization management method, apparatus and equipment | |
| Khedekar | Security: An effective technique to protecting sensitive information using quick response code |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180710 |