CN112550951A - Biological sample storage method and device, computer equipment and storage medium - Google Patents
Biological sample storage method and device, computer equipment and storage medium Download PDFInfo
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- CN112550951A CN112550951A CN202011406926.2A CN202011406926A CN112550951A CN 112550951 A CN112550951 A CN 112550951A CN 202011406926 A CN202011406926 A CN 202011406926A CN 112550951 A CN112550951 A CN 112550951A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D85/00—Containers, packaging elements or packages, specially adapted for particular articles or materials
- B65D85/50—Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage
Abstract
The application relates to a biological sample storage method, a biological sample storage device, a computer device and a storage medium. The method comprises the following steps: acquiring a current storage environment factor corresponding to a current biological sample to be stored, wherein the current storage environment factor comprises a current storage demand factor and a current transfer equipment factor; when the storage mode of the current biological sample to be stored is judged to be stored in the temporary storage area of the device according to the current transfer device factor, acquiring a next biological sample to be stored corresponding to the current biological sample to be stored and a next storage environment factor corresponding to the next biological sample to be stored, wherein the next storage environment factor comprises a next storage demand factor; and re-determining the storage mode corresponding to the current biological sample to be stored according to the current storage demand factor and the next storage demand factor, and performing sample storage on the current biological sample to be stored according to the determined storage mode. The method can improve the storage efficiency of the biological sample.
Description
Technical Field
The present application relates to the field of biological sample storage technologies, and in particular, to a biological sample storage method, apparatus, computer device, and storage medium.
Background
The ultra-low temperature storage device for the automatic biological samples is widely applied to various links of the medical industry, is used for storing various biological macromolecules (DNA, RNA, protein and the like), cells, tissues, organs and other samples, and is used for clinical treatment of diseases and biological application of life science research. For long-term storage of biological samples, the lowest possible temperature is usually used to reduce the biochemical reactions within the sample, in order to increase the stability of the various components within the sample.
The process of storing biological samples (such as cells) in an automated ultra-low temperature storage device is a very critical step, because the biological samples are kept at a low temperature as far as possible during the warehousing process, but when a large batch of biological samples need to be stored in the storage device at the same time, the storage work of the biological samples needs to be sequentially executed in the conventional technology, but in the storage process, the waiting time of the biological samples is inevitably caused, the exposure time of the biological samples in an improper environment is further prolonged, and the stability of the biological samples is at a risk of being reduced.
Disclosure of Invention
In view of the above, there is a need to provide a method, an apparatus, a computer device and a storage medium capable of improving the storage efficiency of biological samples.
A biological sample storage method, the method comprising:
acquiring a current storage environment factor corresponding to a current biological sample to be stored, wherein the current storage environment factor comprises a current storage demand factor and a current transfer equipment factor;
when the storage mode of the current biological sample to be stored is judged to be stored in the temporary storage area of the device according to the current transfer device factor, acquiring a next biological sample to be stored corresponding to the current biological sample to be stored and a next storage environment factor corresponding to the next biological sample to be stored, wherein the next storage environment factor comprises a next storage demand factor;
and re-determining the storage mode corresponding to the current biological sample to be stored according to the current storage demand factor and the next storage demand factor, and performing sample storage on the current biological sample to be stored according to the determined storage mode.
In one embodiment, after obtaining the current storage environment factor corresponding to the current biological sample to be stored, the method further includes:
extracting a current transfer equipment type corresponding to a biological sample to be stored currently from a current transfer equipment factor, wherein the current transfer equipment type is determined according to the number of the biological samples which can be transferred by the transfer equipment at one time;
when the type of the current transfer equipment corresponds to a transfer tank, judging that the storage mode corresponding to the current biological sample to be stored is to be stored in an equipment storage area, wherein the number of the biological samples which can be transferred by the transfer tank at one time is less than a preset threshold value;
when the type of the current transfer equipment corresponds to the transfer box, the storage mode corresponding to the current biological sample to be stored is determined to be stored in the temporary storage area of the equipment, and the number of the biological samples which can be transferred by the transfer box in a single time is larger than or equal to a preset threshold value.
In one embodiment, determining a storage mode corresponding to a current biological sample to be stored according to a current storage requirement factor and a next storage requirement factor, and performing sample storage on the current biological sample to be stored according to the determined storage mode includes:
extracting a current storage temperature factor from the current storage demand factor;
when the current storage temperature factor corresponds to a low-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage device;
and when the current storage temperature factor corresponds to the normal-temperature storage factor, determining the storage mode corresponding to the current biological sample to be stored according to the next storage demand factor.
In one embodiment, determining the storage mode corresponding to the current biological sample to be stored according to the next storage requirement factor includes:
extracting a next storage temperature factor from the next storage demand factor;
when the next storage temperature factor corresponds to a low-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the temporary storage equipment;
and when the next storage temperature factor corresponds to the normal-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage device.
In one embodiment, when the type of the current transfer device corresponds to the transfer box, after determining that the storage mode corresponding to the current biological sample to be stored is stored in the device buffer, the method further includes:
acquiring an equipment data structure corresponding to a transfer box, wherein the equipment data structure is used for identifying the plate frame position information of a transfer plate frame in transfer equipment;
checking the position information of the plate racks according to a preset position checking algorithm, and acquiring the number information of the plate racks corresponding to the temporary storage area of the equipment when the checking is passed;
and checking the plate frame quantity information according to a preset quantity checking algorithm, judging that the temporary storage equipment has a free space for storing the current biological sample to be stored when the checking is passed, and determining that the storage mode corresponding to the current biological sample to be stored is stored in the temporary storage equipment.
In one embodiment, after the grillage number information is checked according to a preset number checking algorithm, the method further includes:
when the verification fails, judging that the temporary storage equipment does not have a free space for storing the current biological sample to be stored, and acquiring the equipment running state of the control equipment;
and when the equipment running state corresponds to a qualified state, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage equipment, and indicating the control equipment to store the current biological sample to be stored in the storage equipment.
In one embodiment, the grillage position information is checked according to a preset position checking algorithm, and when the check is passed, the grillage number information corresponding to the temporary storage area of the device is obtained, including:
respectively checking the area position information and the layer position information in the grillage position information according to a preset position checking algorithm;
and when the area position information and the layer position information are verified, acquiring the number information of the grillage corresponding to the temporary storage area of the equipment.
A biological sample storage device, the device comprising:
the acquisition device is used for acquiring a current storage environment factor corresponding to the current biological sample to be stored, wherein the current storage environment factor comprises a current storage demand factor and a current transfer equipment factor;
the first judging module is used for acquiring a next biological sample to be stored corresponding to the current biological sample to be stored and a next storage environment factor corresponding to the next biological sample to be stored when the storage mode of the current biological sample to be stored is judged to be stored in the temporary storage area of the equipment according to the current transfer equipment factor, wherein the next storage environment factor comprises a next storage demand factor;
and the second judging module is used for re-determining the storage mode corresponding to the current biological sample to be stored according to the current storage demand factor and the next storage demand factor so as to store the sample of the current biological sample to be stored according to the determined storage mode.
A computer device comprising a memory storing a computer program and a processor implementing the steps of the method of any of the embodiments described above when the computer program is executed by the processor.
A computer-readable storage medium, on which a computer program is stored which, when being executed by a processor, carries out the steps of the method of any of the above embodiments.
According to the biological sample storage method, the biological sample storage device, the computer equipment and the storage medium, the current storage environment factor corresponding to the biological sample to be stored currently is obtained, and the current storage environment factor comprises the current storage demand factor and the current transfer equipment factor. And the current transfer equipment factor is used as a condition for judging and storing the current biological sample to be stored, so that a storage mode of a corresponding mode is flexibly selected for the current biological sample to be stored. When the storage mode of the current biological sample to be stored is judged to be stored in the temporary storage area of the device according to the current transfer device factor, acquiring a next biological sample to be stored corresponding to the current biological sample to be stored and a next storage environment factor corresponding to the next biological sample to be stored, wherein the next storage environment factor comprises a next storage demand factor; and determining a storage mode corresponding to the current biological sample to be stored according to the current storage demand factor and the next storage demand factor, and performing sample storage on the current biological sample to be stored according to the determined storage mode. By providing various types of biological sample storage devices, the storage device which is adaptive to the current biological sample to be stored can be dynamically determined according to the current storage environment factor, the purpose of flexibly storing the current biological sample is achieved, more device selections are provided for storing the current biological sample, the waiting time of the biological sample to be stored is shortened, and the biological activity of the current biological sample to be stored is further ensured.
Drawings
FIG. 1 is a diagram of an exemplary environment in which a method for storing a biological sample may be used;
FIG. 2 is a schematic flow chart of a biological sample storage method according to one embodiment;
FIG. 3 is a flow chart illustrating a biological sample storage selection process provided in one embodiment;
FIG. 4 is a schematic flow chart illustrating a determination process for a biological sample storage device according to one embodiment;
FIG. 5 is a block diagram of the structure of a biological sample storage device in one embodiment;
FIG. 6 is a diagram illustrating an internal structure of a computer device according to an embodiment.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.
The biological sample storage method provided by the application can be applied to the application environment shown in fig. 1. Wherein the terminal 102 communicates with the server 104 via a network. The server 104 acquires a current biological sample to be stored sent by the terminal 102, and acquires a current storage environment factor corresponding to the current biological sample to be stored, wherein the current storage environment factor comprises a current storage demand factor and a current transfer equipment factor; when the storage mode of the current biological sample to be stored is judged to be stored in the temporary storage area of the device according to the current transfer device factor, acquiring a next biological sample to be stored corresponding to the current biological sample to be stored and a next storage environment factor corresponding to the next biological sample to be stored, wherein the next storage environment factor comprises a next storage demand factor; and determining a storage mode corresponding to the current biological sample to be stored according to the current storage demand factor and the next storage demand factor, and performing sample storage on the current biological sample to be stored according to the determined storage mode. The terminal 102 may be, but not limited to, various personal computers, notebook computers, smart phones, tablet computers, and portable wearable devices, and the server 104 may be implemented by an independent server or a server cluster formed by a plurality of servers.
In one embodiment, as shown in fig. 2, a biological sample storage method is provided, which is exemplified by the application of the method to the server in fig. 1, and comprises the following steps:
The biological sample comprises biological cells with biological activity, and the current storage environment factor is a factor element for storing the biological sample, and specifically can comprise a current storage requirement factor and a current transportation equipment factor. The current storage demand factor comprises information such as ambient temperature and ambient humidity required for storing the biological sample, and the current transfer equipment factor comprises transfer equipment for transferring the biological sample, and specifically can comprise a current transfer equipment type of the transfer equipment. Specifically, current transfer equipment type includes transfer case and transfer tank etc. when including the transfer grillage in the transfer equipment, can also include the equipment data structure of transfer equipment in the current transfer equipment factor, and equipment data structure is used for the grillage position information of the transfer grillage in the sign transfer equipment.
Specifically, when the server receives the biological sample to be stored, a warehousing entry corresponding to the biological sample to be stored is obtained, and the warehousing entry includes a current environmental factor corresponding to the biological sample to be stored currently.
And 204, when the storage mode of the current biological sample to be stored is judged to be stored in the temporary storage area of the device according to the current transfer device factor, acquiring a next biological sample to be stored corresponding to the current biological sample to be stored and a next storage environment factor corresponding to the next biological sample to be stored, wherein the next storage environment factor comprises a next storage requirement factor.
Specifically, the storage mode of the current biological sample to be stored may be determined according to the type of the transfer device in the current conversion device factor, or may also be determined according to the number of samples currently matching the biological sample to be warehoused. In a specific embodiment, if the number of samples of the biological sample to be stored in the current batch is small, the biological sample to be stored in the current batch can be stored into the storage device all at once, so as to ensure the optimal biological activity of the biological sample, if the number of samples of the biological sample to be stored in the current batch is large, a part of the biological sample to be stored can be temporarily stored into the temporary storage device, so as to temporarily stabilize the sample activity of the biological sample, so as to reduce the time that the biological sample is exposed in an adverse environment, and when the device storage area has a space, the biological sample in the temporary storage device can be transferred into the device storage area, so as to ensure the sample activity of the biological sample in the whole warehousing process to the largest possible extent.
The temporary device storage area is an area environment for temporarily storing the biological sample, and when the biological sample is stored in the temporary device storage area for a certain time, the biological sample needs to be transferred to the device storage area, so that the biological sample can be stably stored in the device storage area for a long time. The temporary storage area of the equipment is an area which can provide a storage environment within a certain time, and particularly can provide a low-temperature storage environment or a normal-temperature storage environment. The device storage area is an area that can provide an optimal storage environment for the biological sample, and is a device that can store the biological sample for a long time.
In a specific application environment, when a plurality of biological samples need to be stored in the storage device, the biological samples need to be stored in the storage device one by one, the biological samples sorted at the current time to be put in storage are used as the biological samples to be stored currently, and the biological samples sorted at the current time to be put in storage are used as the biological samples to be stored next. The next storage requirement factor includes information such as ambient temperature, ambient humidity, etc. required to store the next biological sample.
And step 206, re-determining the storage mode corresponding to the current biological sample to be stored according to the current storage requirement factor and the next storage requirement factor, and performing sample storage on the current biological sample to be stored according to the determined storage mode.
In a specific embodiment, the storage mode of the current biological sample to be stored can be determined according to the required storage environment of the next biological sample to be stored. Specifically, when the storage temperature of the next biological sample to be stored is low and the storage environment corresponding to the current biological sample to be stored is normal temperature storage, the current biological sample to be stored may be stored in the temporary storage area, and the next biological sample to be stored is preferentially stored in the device storage area.
Ultra (deep) cryogenic storage devices for biological samples (cells) are increasingly used in various segments of the medical industry for storing samples of various biological macromolecules (DNA, RNA, proteins, etc.), cells, tissues, organs, etc. for clinical treatment of diseases and biological applications for life science research. For long-term storage of biological samples, the lowest possible temperature is usually used to reduce the biochemical reactions within the sample, in order to increase the stability of the various components within the sample. The process of storing biological samples (cells) into an automatic ultra (deep) low temperature storage device (warehousing process) is a very critical step, because the biological samples (cells)) are kept at a continuous low temperature as much as possible in the warehousing process, but for the warehousing requirement that tens or even hundreds of freezing storage tubes need to be stored frequently at one time, how to rapidly, safely and stably warehouse the biological samples (cells) becomes very important.
According to the biological sample storage method, the current storage environment factor corresponding to the biological sample to be stored currently is obtained, and the current storage environment factor comprises the current storage demand factor and the current transfer equipment factor. And the current transfer equipment factor is used as a condition for judging and storing the current biological sample to be stored, so that a storage mode of a corresponding mode is flexibly selected for the current biological sample to be stored. When the storage mode of the current biological sample to be stored is judged to be stored in the temporary storage area of the device according to the current transfer device factor, acquiring a next biological sample to be stored corresponding to the current biological sample to be stored and a next storage environment factor corresponding to the next biological sample to be stored, wherein the next storage environment factor comprises a next storage demand factor; and determining a storage mode corresponding to the current biological sample to be stored according to the current storage demand factor and the next storage demand factor, and performing sample storage on the current biological sample to be stored according to the determined storage mode. By providing various types of biological sample storage devices, the storage device which is adaptive to the current biological sample to be stored can be dynamically determined according to the current storage environment factor, the purpose of flexibly storing the current biological sample is achieved, more device selections are provided for storing the current biological sample, the waiting time of the biological sample to be stored is shortened, and the biological activity of the current biological sample to be stored is further ensured.
In one embodiment, after obtaining the current storage environment factor corresponding to the current biological sample to be stored, the method further includes: extracting a current transfer equipment type corresponding to a biological sample to be stored currently from a current transfer equipment factor, wherein the current transfer equipment type is determined according to the number of the biological samples which can be transferred by the transfer equipment at one time; when the type of the current transfer equipment corresponds to a transfer tank, judging that the storage mode corresponding to the current biological sample to be stored is to be stored in an equipment storage area, wherein the number of the biological samples which can be transferred by the transfer tank at one time is less than a preset threshold value; when the type of the current transfer equipment corresponds to the transfer box, the storage mode corresponding to the current biological sample to be stored is determined to be stored in the temporary storage area of the equipment, and the number of the biological samples which can be transferred by the transfer box in a single time is larger than or equal to a preset threshold value.
The biological samples can be placed in the same or different transfer plate racks, and the transfer plate racks can be sample trays for storing the biological samples. And the position of the biological sample in the biological transfer plate frame is not limited, and the size and the shape of the sample tray are not limited. For example, the sample tray may be a 6 × 8 tray, that is, the sample tray may store 48 biological samples. In particular, a plurality of sample trays may be placed simultaneously in the transfer device. And, can confirm the transfer device type that the transfer device corresponds according to the quantity of the sample tray that can place in the transfer device, can confirm current transfer device type according to the quantity of the biological sample that transfer device single can transport specifically, when the quantity of the biological sample that transfer device single can transport is less than and predetermines the threshold value, judge that the transfer device type that current transfer device corresponds is the transfer tank, when the quantity of the biological sample that transfer device single can transport is greater than or is greater than and predetermines the threshold value, judge that the transfer device type that current transfer device corresponds is the transfer box.
In a specific embodiment, when the type of the transfer device for transferring the current biological sample is a transfer box, since the number of biological samples that can be transferred by the transfer box at a time is greater than or equal to the preset threshold, that is, the number of biological samples that can be transferred by the transfer box at the time is large, since the current device to be stored cannot be stored to the device storage area at a time, the storage needs to be matched, so as to reduce the waiting time between different batches of biological samples, a part of the batches of biological samples can be stored to the device buffer area first, so as to ensure the biological activity of the biological samples during the process of transferring the biological samples.
In a specific embodiment, the ultra (deep) low temperature storage device for the automatic biological samples (cells) already supports the function of storing the whole transfer plate frame, including the freezing tubes on the transfer plate frame, in a warehouse at one time, so that the warehousing speed of the biological samples (cells) is greatly improved. The automatic ultra (deep) low temperature storage device for biological samples (cells) has a speed shortage for the operation of warehousing a batch of a plurality of transfer plate frames, and needs to be greatly improved for biological samples (cells) with strict requirements on transfer and storage temperatures. In one embodiment, the transfer tank is corresponding to include a transfer grillage, so can realize once only transferring the biological sample to storage equipment, the transfer box is corresponding to including a plurality of transfer grillages, so can't realize once only transferring the biological sample to storage equipment, so can be with some biological samples in the equipment buffer of keeping in earlier to guarantee biological sample's biological activity.
In one embodiment, determining a storage mode corresponding to a current biological sample to be stored according to a current storage requirement factor and a next storage requirement factor, and performing sample storage on the current biological sample to be stored according to the determined storage mode includes: extracting a current storage temperature factor from the current storage demand factor; when the current storage temperature factor corresponds to a low-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage device; and when the current storage temperature factor corresponds to the normal-temperature storage factor, determining the storage mode corresponding to the current biological sample to be stored according to the next storage demand factor.
As shown in fig. 3, fig. 3 is a flow chart for selecting a biological sample storage method provided in an embodiment, where in fig. 3, the warehousing method switching condition includes determining a warehousing method according to a device type of current transfer equipment, for example, according to a transfer tank or a transfer box, and the warehousing method switching condition further includes determining a warehousing method according to a device type of current transfer equipment and a current storage temperature factor, for example, a requirement of a warehousing cryopreservation pipe on a temperature in a warehousing process.
In the embodiment, a solution for rapidly warehousing biological samples in a batch of multi-transfer plate racks is provided; the method and the device realize that how to execute the warehousing operation mode can be dynamically determined according to the condition of the subsequent to-be-warehoused sample, and realize a solution for switching multiple warehousing modes according to actual needs.
In one embodiment, determining the storage mode corresponding to the current biological sample to be stored according to the next storage requirement factor includes: extracting a next storage temperature factor from the next storage demand factor; when the next storage temperature factor corresponds to a low-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the temporary storage equipment; and when the next storage temperature factor corresponds to the normal-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage device.
Specifically, if the type of the transfer device is determined to be a transfer tank, the storage of the biological sample to be stored in the device storage area can be directly performed in consideration of the fact that the transfer tank is only provided with one transfer rack. If the transfer equipment is a transfer box provided with a plurality of transfer plate frames, and the warehousing process of the biological samples needs a low-temperature state, the biological samples can be directly stored in the equipment storage area at the moment. In another embodiment, the device buffer may provide a low temperature environment, so that the biological sample may be stored in the device buffer, so that the device buffer provides a low temperature environment, and then transferred from the device buffer to the device storage area.
The temporary storage area of the device can provide a low-temperature or non-low-temperature environment, on the one hand, the temperature environment can be provided, the capacity of the storage area of the device for storing the biological samples is enlarged, on the other hand, the biological samples can be quickly put into a warehouse, and at the moment, the time for the next biological sample to be put into the warehouse queued at the back is shortened, so that the time for the biological sample to be put into the warehouse in the adverse environment is prevented from being too long, and the activity of the biological sample is reduced. And when the transfer plate racks to be put in storage are firstly stored in the temporary storage area of the equipment, after all the transfer plate racks to be put in storage are placed in the temporary storage area of the equipment, the transfer plate racks are taken out from the temporary storage area of the equipment, and the next step of transferring the biological samples to be stored is executed.
In one embodiment, when the type of the current transfer device corresponds to the transfer box, after determining that the storage mode corresponding to the current biological sample to be stored is stored in the device buffer, the method further includes: acquiring an equipment data structure corresponding to a transfer box, wherein the equipment data structure is used for identifying the plate frame position information of a transfer plate frame in transfer equipment; checking the position information of the plate racks according to a preset position checking algorithm, and acquiring the number information of the plate racks corresponding to the temporary storage area of the equipment when the checking is passed; and checking the plate frame quantity information according to a preset quantity checking algorithm, judging that the temporary storage equipment has a free space for storing the current biological sample to be stored when the checking is passed, and determining that the storage mode corresponding to the current biological sample to be stored is stored in the temporary storage equipment.
Wherein, the grillage position information of transporting the grillage is used for the position of sign transportation grillage in the transfer apparatus. Specifically, can carry out the subregion with the transfer equipment according to the specification of transporting the grillage, if can be according to transporting the parameters such as the bottom area of grillage and divide into a plurality of regions with the transfer equipment to mark as regional A, regional B, regional C and regional D etc.. And then determining the number of layers of the transfer plate rack, such as layer 1, layer 2, layer 3 and the like, which can be stored in each region according to the height information of each region, the height of the transfer plate rack and other parameters. For example, the device data structure may be in an array form, and in the corresponding array, the position of different data corresponds to a position of a transportation rack, for example, a01 represents the first layer in the area a, and B01 represents the first layer in the area B. As shown in equation (1), an array representation of a device data structure is provided.
And it should be noted that each layer of the transfer board frame is stored in an overlapping way, if the lowest layer in a region has no data, the data in the upper layer cannot exist, otherwise, an error occurs.
In one embodiment, the grillage position information is checked according to a preset position checking algorithm, and when the check is passed, the grillage number information corresponding to the temporary storage area of the device is obtained, including: respectively checking the area position information and the layer position information in the grillage position information according to a preset position checking algorithm; and when the area position information and the layer position information are verified, acquiring the number information of the grillage corresponding to the temporary storage area of the equipment.
The area position information is information for representing the area of the transfer plate frame in the transfer equipment, and the layer position information is information for representing the layer where the transfer plate frame is located. The position checking algorithm is used for checking the array corresponding to the equipment data structure, and the equipment data structure represents the grillage position information, so that the grillage position information can be checked through checking the array structure. The data size of the data at each position in the array is checked, the position relation between the data is checked, and the dependency relation between different data is checked. If the data corresponding to the lower shelf is empty, but the data corresponding to the corresponding upper shelf is data, it is indicated that the dependency relationship between the data and the lower shelf is wrong, and it can be determined that the device data structure is wrong at this time, that is, the verification fails.
The information of the number of the grillage is the number of the biological samples which can be stored on the grillage, in one embodiment, the number of the biological samples which can be stored on the grillage at most is known to be 24 in advance, but the number of the biological samples at the moment is verified to be 27 through a data verification algorithm, so that the error exists at the moment, and the notification of the failure of the number verification is sent out. In one embodiment, when the position and the number are verified, it may be determined again whether there is a free space in the buffer device, and if it is determined that the buffer device has a free space for storing the current biological sample to be stored, it is determined that the current biological sample to be stored is stored in the buffer device in the corresponding storage manner.
In one embodiment, after the grillage number information is checked according to a preset number checking algorithm, the method further includes: when the verification fails, judging that the temporary storage equipment does not have a free space for storing the current biological sample to be stored, and acquiring the equipment running state of the control equipment; and when the equipment running state corresponds to a qualified state, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage equipment, and indicating the control equipment to store the current biological sample to be stored in the storage equipment.
Free space means that there is space in the staging device for storing biological samples. The running state of the control equipment refers to the running condition of the control equipment at the moment, and specifically can include whether the control equipment has normal working conditions or not, wherein the working conditions comprise working temperature, running parameters and the like, when the running state of the control equipment is qualified, the control equipment is identified to be in a qualified state, the control equipment can be executed to execute the transfer of the plate racks in the temporary storage equipment to the equipment storage area, and the plate racks placed in the temporary storage area are stored in the equipment storage area.
In a specific embodiment, as shown in fig. 4, fig. 4 is a schematic view of a biological sample storage device determination process provided in an embodiment, in fig. 4, when a server receives a biological sample to be stored, first determining a device type of a transportation device for transporting the biological sample to be stored, and when the device type is not a transportation box, performing storage of a transportation plate rack corresponding to the biological sample to a device storage area. When the equipment type corresponds to the transfer box, the storage temperature that should wait to store biological sample and correspond is judged next, and when storage temperature corresponded the normal atmospheric temperature operation at that time, the execution will be transported and wait to store biological sample's transfer grillage storage to equipment temporary storage area. And then continuously judging the equipment running state of the control equipment, when the equipment runs normally, indicating that the control equipment is in an idle state, executing to store the transfer plate rack corresponding to the biological sample into an equipment storage area, otherwise, continuously waiting for the control equipment, and executing to store the transfer plate rack corresponding to the biological sample into the equipment storage area until the control equipment is in the idle state.
It should be understood that although the various steps in the flow charts of fig. 2-4 are shown in order as indicated by the arrows, the steps are not necessarily performed in order as indicated by the arrows. The steps are not performed in the exact order shown and described, and may be performed in other orders, unless explicitly stated otherwise. Moreover, at least some of the steps in fig. 2-4 may include multiple steps or multiple stages, which are not necessarily performed at the same time, but may be performed at different times, which are not necessarily performed in sequence, but may be performed in turn or alternately with other steps or at least some of the other steps.
In one embodiment, as shown in fig. 5, there is provided a biological sample storage device comprising:
the obtaining device 502 is configured to obtain a current storage environment factor corresponding to a current biological sample to be stored, where the current storage environment factor includes a current storage requirement factor and a current transfer equipment factor.
The first determining module 504 is configured to, when it is determined that the storage manner of the current biological sample to be stored is to be stored in the device buffer according to the current transfer device factor, obtain a next biological sample to be stored corresponding to the current biological sample to be stored and a next storage environment factor corresponding to the next biological sample to be stored, where the next storage environment factor includes a next storage requirement factor.
The second judging module 506 is configured to re-determine the storage mode corresponding to the current biological sample to be stored according to the current storage requirement factor and the next storage requirement factor, so as to perform sample storage on the current biological sample to be stored according to the determined storage mode.
In one embodiment, the biological sample storage device further comprises a type discrimination module, wherein the type discrimination module is used for extracting a current transfer equipment type corresponding to the biological sample to be stored currently from the current transfer equipment factor, and the current transfer equipment type is determined according to the number of the biological samples which can be transferred by the transfer equipment at a single time; when the type of the current transfer equipment corresponds to a transfer tank, judging that the storage mode corresponding to the current biological sample to be stored is to be stored in an equipment storage area, wherein the number of the biological samples which can be transferred by the transfer tank at one time is less than a preset threshold value; when the type of the current transfer equipment corresponds to the transfer box, the storage mode corresponding to the current biological sample to be stored is determined to be stored in the temporary storage area of the equipment, and the number of the biological samples which can be transferred by the transfer box in a single time is larger than or equal to a preset threshold value.
In one embodiment, the second determination module 506 is further configured to extract a current storage temperature factor from the current storage demand factor; when the current storage temperature factor corresponds to a low-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage device; and when the current storage temperature factor corresponds to the normal-temperature storage factor, determining the storage mode corresponding to the current biological sample to be stored according to the next storage demand factor.
In one embodiment, the second determination module 506 is further configured to extract a next storage temperature factor from the next storage demand factor; when the next storage temperature factor corresponds to a low-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the temporary storage equipment; and when the next storage temperature factor corresponds to the normal-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage device.
In one embodiment, the biological sample storage device further comprises a checking module, wherein the checking module is used for acquiring an equipment data structure corresponding to the transfer box, and the equipment data structure is used for identifying the pallet position information of the transfer pallet in the transfer equipment; checking the position information of the plate racks according to a preset position checking algorithm, and acquiring the number information of the plate racks corresponding to the temporary storage area of the equipment when the checking is passed; and checking the plate frame quantity information according to a preset quantity checking algorithm, judging that the temporary storage equipment has a free space for storing the current biological sample to be stored when the checking is passed, and determining that the storage mode corresponding to the current biological sample to be stored is stored in the temporary storage equipment.
In one embodiment, the biological sample storage device further comprises a state discrimination module, wherein the state discrimination module is used for determining that the temporary storage device does not have a free space for storing the biological sample to be stored currently when the verification fails, and acquiring the device running state of the control device; and when the equipment running state corresponds to a qualified state, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage equipment, and indicating the control equipment to store the current biological sample to be stored in the storage equipment.
In one embodiment, the checking module is further configured to check the area position information and the hierarchical position information in the grillage position information according to a preset position checking algorithm; and when the area position information and the layer position information are verified, acquiring the number information of the grillage corresponding to the temporary storage area of the equipment.
For the specific definition of the biological sample storage device, reference may be made to the above definition of the biological sample storage method, which is not described herein again. The various modules of the biological sample storage device described above may be implemented in whole or in part by software, hardware, and combinations thereof. The modules can be embedded in a hardware form or independent from a processor in the computer device, and can also be stored in a memory in the computer device in a software form, so that the processor can call and execute operations corresponding to the modules.
In one embodiment, a computer device is provided, which may be a server, and its internal structure diagram may be as shown in fig. 6. The computer device includes a processor, a memory, and a network interface connected by a system bus. Wherein the processor of the computer device is configured to provide computing and control capabilities. The memory of the computer device comprises a nonvolatile storage medium and an internal memory. The non-volatile storage medium stores an operating system, a computer program, and a database. The internal memory provides an environment for the operation of an operating system and computer programs in the non-volatile storage medium. The database of the computer device is for storing biological sample data. The network interface of the computer device is used for communicating with an external terminal through a network connection. The computer program is executed by a processor to implement a biological sample storage method.
Those skilled in the art will appreciate that the architecture shown in fig. 6 is merely a block diagram of some of the structures associated with the disclosed aspects and is not intended to limit the computing devices to which the disclosed aspects apply, as particular computing devices may include more or less components than those shown, or may combine certain components, or have a different arrangement of components.
In one embodiment, a computer device is provided, comprising a memory and a processor, the memory having a computer program stored therein, the processor implementing the following steps when executing the computer program: acquiring a current storage environment factor corresponding to a current biological sample to be stored, wherein the current storage environment factor comprises a current storage demand factor and a current transfer equipment factor; when the storage mode of the current biological sample to be stored is judged to be stored in the temporary storage area of the device according to the current transfer device factor, acquiring a next biological sample to be stored corresponding to the current biological sample to be stored and a next storage environment factor corresponding to the next biological sample to be stored, wherein the next storage environment factor comprises a next storage demand factor; and re-determining the storage mode corresponding to the current biological sample to be stored according to the current storage demand factor and the next storage demand factor, and performing sample storage on the current biological sample to be stored according to the determined storage mode.
In one embodiment, the processor, when executing the computer program, further performs the steps of: extracting a current transfer equipment type corresponding to a biological sample to be stored currently from a current transfer equipment factor, wherein the current transfer equipment type is determined according to the number of the biological samples which can be transferred by the transfer equipment at one time; when the type of the current transfer equipment corresponds to a transfer tank, judging that the storage mode corresponding to the current biological sample to be stored is to be stored in an equipment storage area, wherein the number of the biological samples which can be transferred by the transfer tank at one time is less than a preset threshold value; when the type of the current transfer equipment corresponds to the transfer box, the storage mode corresponding to the current biological sample to be stored is determined to be stored in the temporary storage area of the equipment, and the number of the biological samples which can be transferred by the transfer box in a single time is larger than or equal to a preset threshold value.
In one embodiment, the processor, when executing the computer program, further performs the steps of: extracting a current storage temperature factor from the current storage demand factor; when the current storage temperature factor corresponds to a low-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage device; and when the current storage temperature factor corresponds to the normal-temperature storage factor, determining the storage mode corresponding to the current biological sample to be stored according to the next storage demand factor.
In one embodiment, the processor, when executing the computer program, further performs the steps of: extracting a next storage temperature factor from the next storage demand factor; when the next storage temperature factor corresponds to a low-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the temporary storage equipment; and when the next storage temperature factor corresponds to the normal-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage device.
In one embodiment, the processor, when executing the computer program, further performs the steps of: acquiring an equipment data structure corresponding to a transfer box, wherein the equipment data structure is used for identifying the plate frame position information of a transfer plate frame in transfer equipment; checking the position information of the plate racks according to a preset position checking algorithm, and acquiring the number information of the plate racks corresponding to the temporary storage area of the equipment when the checking is passed; and checking the plate frame quantity information according to a preset quantity checking algorithm, judging that the temporary storage equipment has a free space for storing the current biological sample to be stored when the checking is passed, and determining that the storage mode corresponding to the current biological sample to be stored is stored in the temporary storage equipment.
In one embodiment, the processor, when executing the computer program, further performs the steps of: when the verification fails, judging that the temporary storage equipment does not have a free space for storing the current biological sample to be stored, and acquiring the equipment running state of the control equipment; and when the equipment running state corresponds to a qualified state, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage equipment, and indicating the control equipment to store the current biological sample to be stored in the storage equipment.
In one embodiment, the processor, when executing the computer program, further performs the steps of: check-up grillage positional information according to predetermined position check-up algorithm, acquire the grillage quantity information that equipment temporary storage corresponds when the check-up passes, include: respectively checking the area position information and the layer position information in the grillage position information according to a preset position checking algorithm; and when the area position information and the layer position information are verified, acquiring the number information of the grillage corresponding to the temporary storage area of the equipment.
In one embodiment, a computer-readable storage medium is provided, having a computer program stored thereon, which when executed by a processor, performs the steps of: acquiring a current storage environment factor corresponding to a current biological sample to be stored, wherein the current storage environment factor comprises a current storage demand factor and a current transfer equipment factor; when the storage mode of the current biological sample to be stored is judged to be stored in the temporary storage area of the device according to the current transfer device factor, acquiring a next biological sample to be stored corresponding to the current biological sample to be stored and a next storage environment factor corresponding to the next biological sample to be stored, wherein the next storage environment factor comprises a next storage demand factor; and re-determining the storage mode corresponding to the current biological sample to be stored according to the current storage demand factor and the next storage demand factor, and performing sample storage on the current biological sample to be stored according to the determined storage mode.
In one embodiment, the computer program when executed by the processor further performs the steps of: extracting a current transfer equipment type corresponding to a biological sample to be stored currently from a current transfer equipment factor, wherein the current transfer equipment type is determined according to the number of the biological samples which can be transferred by the transfer equipment at one time; when the type of the current transfer equipment corresponds to a transfer tank, judging that the storage mode corresponding to the current biological sample to be stored is to be stored in an equipment storage area, wherein the number of the biological samples which can be transferred by the transfer tank at one time is less than a preset threshold value; when the type of the current transfer equipment corresponds to the transfer box, the storage mode corresponding to the current biological sample to be stored is determined to be stored in the temporary storage area of the equipment, and the number of the biological samples which can be transferred by the transfer box in a single time is larger than or equal to a preset threshold value.
In one embodiment, the computer program when executed by the processor further performs the steps of: extracting a current storage temperature factor from the current storage demand factor; when the current storage temperature factor corresponds to a low-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage device; and when the current storage temperature factor corresponds to the normal-temperature storage factor, determining the storage mode corresponding to the current biological sample to be stored according to the next storage demand factor.
In one embodiment, the computer program when executed by the processor further performs the steps of: extracting a next storage temperature factor from the next storage demand factor; when the next storage temperature factor corresponds to a low-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the temporary storage equipment; and when the next storage temperature factor corresponds to the normal-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage device.
In one embodiment, the computer program when executed by the processor further performs the steps of: acquiring an equipment data structure corresponding to a transfer box, wherein the equipment data structure is used for identifying the plate frame position information of a transfer plate frame in transfer equipment; checking the position information of the plate racks according to a preset position checking algorithm, and acquiring the number information of the plate racks corresponding to the temporary storage area of the equipment when the checking is passed; and checking the plate frame quantity information according to a preset quantity checking algorithm, judging that the temporary storage equipment has a free space for storing the current biological sample to be stored when the checking is passed, and determining that the storage mode corresponding to the current biological sample to be stored is stored in the temporary storage equipment.
In one embodiment, the computer program when executed by the processor further performs the steps of: when the verification fails, judging that the temporary storage equipment does not have a free space for storing the current biological sample to be stored, and acquiring the equipment running state of the control equipment; and when the equipment running state corresponds to a qualified state, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the storage equipment, and indicating the control equipment to store the current biological sample to be stored in the storage equipment.
In one embodiment, the computer program when executed by the processor further performs the steps of: check-up grillage positional information according to predetermined position check-up algorithm, acquire the grillage quantity information that equipment temporary storage corresponds when the check-up passes, include: respectively checking the area position information and the layer position information in the grillage position information according to a preset position checking algorithm; and when the area position information and the layer position information are verified, acquiring the number information of the grillage corresponding to the temporary storage area of the equipment.
It will be understood by those skilled in the art that all or part of the processes of the methods of the embodiments described above can be implemented by hardware related to instructions of a computer program, which can be stored in a non-volatile computer-readable storage medium, and when executed, can include the processes of the embodiments of the methods described above. Any reference to memory, storage, database or other medium used in the embodiments provided herein can include at least one of non-volatile and volatile memory. Non-volatile Memory may include Read-Only Memory (ROM), magnetic tape, floppy disk, flash Memory, optical storage, or the like. Volatile Memory can include Random Access Memory (RAM) or external cache Memory. By way of illustration and not limitation, RAM can take many forms, such as Static Random Access Memory (SRAM) or Dynamic Random Access Memory (DRAM), among others.
The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above examples only express several embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A method of storing a biological sample, the method comprising:
acquiring a current storage environment factor corresponding to a current biological sample to be stored, wherein the current storage environment factor comprises a current storage demand factor and a current transfer equipment factor;
when the storage mode of the current biological sample to be stored is judged to be stored to the temporary storage area of the device according to the current transfer device factor, acquiring a next biological sample to be stored corresponding to the current biological sample to be stored and a next storage environment factor corresponding to the next biological sample to be stored, wherein the next storage environment factor comprises a next storage demand factor;
and re-determining a storage mode corresponding to the current biological sample to be stored according to the current storage demand factor and the next storage demand factor, and performing sample storage on the current biological sample to be stored according to the determined storage mode.
2. The method of claim 1, wherein after obtaining the current storage environment factor corresponding to the current biological sample to be stored, the method further comprises:
extracting a current transfer equipment type corresponding to the biological sample to be stored currently from the current transfer equipment factor, wherein the current transfer equipment type is determined according to the number of the biological samples which can be transferred by the transfer equipment at one time;
when the type of the current transfer equipment corresponds to a transfer tank, judging that the storage mode corresponding to the current biological sample to be stored is to be stored in an equipment storage area, wherein the number of the biological samples which can be transferred by the transfer tank at one time is less than a preset threshold value;
when the type of the front transfer equipment corresponds to a transfer box, determining that the storage mode corresponding to the current biological sample to be stored is stored to the temporary storage area of the equipment, wherein the number of the biological samples which can be transferred by the transfer box at a time is larger than or equal to a preset threshold value.
3. The method according to claim 1, wherein the determining a storage manner corresponding to the current biological sample to be stored according to the current storage requirement factor and the next storage requirement factor to store the sample of the current biological sample to be stored according to the determined storage manner comprises:
extracting a current storage temperature factor from the current storage demand factor;
when the current storage temperature factor corresponds to a low-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in a storage device;
and when the current storage temperature factor corresponds to a normal-temperature storage factor, determining a storage mode corresponding to the current biological sample to be stored according to the next storage demand factor.
4. The method according to claim 3, wherein the determining the storage mode corresponding to the current biological sample to be stored according to the next storage requirement factor comprises:
extracting a next storage temperature factor from the next storage demand factor;
when the next storage temperature factor corresponds to a low-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in a temporary storage device;
and when the next storage temperature factor corresponds to a normal-temperature storage factor, determining that the storage mode corresponding to the current biological sample to be stored is to store the biological sample to a storage device.
5. The method according to claim 2, wherein when the type of the front transfer device corresponds to a transfer box, after determining that the current biological sample to be stored corresponds to a storage mode of storing the biological sample to a temporary storage area of the device, the method further comprises:
acquiring an equipment data structure corresponding to the transfer box, wherein the equipment data structure is used for identifying the plate rack position information of the transfer plate rack in the transfer equipment;
checking the grillage position information according to a preset position checking algorithm, and acquiring grillage quantity information corresponding to the equipment temporary storage area when the checking is passed;
and checking the plate frame quantity information according to a preset quantity checking algorithm, judging that the temporary storage equipment has a free space for storing the current biological sample to be stored when the checking is passed, and determining that the storage mode corresponding to the current biological sample to be stored is to be stored in the temporary storage equipment.
6. The method of claim 5, wherein after said checking said rack quantity information according to a preset quantity checking algorithm, said method further comprises:
when the verification fails, judging that the temporary storage equipment does not have a free space for storing the current biological sample to be stored, and acquiring the equipment running state of the control equipment;
and when the device running state corresponds to a qualified state, determining that the storage mode corresponding to the current biological sample to be stored is to be stored in a storage device, and indicating the control device to store the current biological sample to be stored in the storage device.
7. The method according to claim 5, wherein the checking the position information of the grillage according to a preset position checking algorithm, and when the checking is passed, acquiring the information of the number of grillages corresponding to the temporary storage area of the device, comprises:
respectively checking the area position information and the layer position information in the grillage position information according to a preset position checking algorithm;
and when the region position information and the layer position information are verified, executing to acquire the number information of the grillage corresponding to the temporary storage area of the equipment.
8. A biological specimen storage device, the device comprising:
the acquisition device is used for acquiring current storage environment factors corresponding to the current biological samples to be stored, wherein the current storage environment factors comprise current storage demand factors and current transfer equipment factors;
the first judging module is used for acquiring a next biological sample to be stored corresponding to the current biological sample to be stored and a next storage environment factor corresponding to the next biological sample to be stored when the storage mode of the current biological sample to be stored is judged to be stored to be a temporary storage area according to the current transfer equipment factor, and the next storage environment factor comprises a next storage requirement factor;
and the second judging module is used for re-determining the storage mode corresponding to the current biological sample to be stored according to the current storage demand factor and the next storage demand factor so as to store the sample of the current biological sample to be stored according to the determined storage mode.
9. A computer device comprising a memory and a processor, the memory storing a computer program, characterized in that the processor, when executing the computer program, implements the steps of the method of any of claims 1 to 7.
10. A computer-readable storage medium, on which a computer program is stored, which, when being executed by a processor, carries out the steps of the method of any one of claims 1 to 7.
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