CN108260522A - A kind of molecular breeding method of high yielding soybeans new lines - Google Patents
A kind of molecular breeding method of high yielding soybeans new lines Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The invention discloses a kind of molecular breeding methods of high yielding soybeans new lines.This method includes：Using genotype be Ln and with non-Four-seed pod character popularization soybean varieties as receptor, using genotype as ln and with Four-seed pod ratio be apparently higher than receptor soybean varieties be donor carry out sexual hybridization；Using receptor as recurrent parent, the progeny soybean single plant continuous backcross with genotype is ln obtains backcross progeny F1BCn；It selects genotype for ln and is apparently higher than the progeny soybean single plant of receptor and 100-grain weight not less than the character of receptor with Four-seed pod ratio, continuous selfing to character inheritance is stablized, and obtains self progeny FnBCn, the strain of character that genotype is apparently higher than receptor for ln and with Four-seed pod ratio is New soybean lines.Compared with as the receptor for promoting soybean varieties, the Four-seed pod ratio and per mu yield of the high yielding soybeans new lines of this method selection and breeding are significantly increased.The present invention has important application value.
Description
Technical field
The invention belongs to field of plant breeding, and in particular to a kind of molecular breeding method of high yielding soybeans new lines.
Background technology
High yield is the long-term main goal of attack of soybean breeder man.Soybean kernel yield is mainly by number of plants per unit area, single plant grain
Number and 100-grain weight determine that wherein single-strain grain number is one of main improvement object of SOYBEAN IN HIGH-YIELD BREEDING, and single-strain grain number is multiplied by single-strain legumen number
With every pod be averaged grain number obtain.The different grain number beanpods of common the cultivated soybean kind include one seeded pod, two pods, three pods and four
Grain pod (five pods seldom see), one seeded pod and two pods are few grain pod type, and three pods and Four-seed pod are multi pods type.
Soybean pod grain number character controls (Chen Yi, the genetic analysis of Seed Number Per Pod In Soybean by 10 or more quantitative trait locus
Soybean Science, 1998 (2)：115-119；Domingo, J.Agric.Res., 1934), wherein including 1 control pod grain number and leaf
Shape character main effect site (Chao Fang et al., Journal of Genetics and Genomics, 2013；
Sawada, Soybean Genet.Newsl., 1988).In soybean planting density (i.e. number of plants per unit area) and 100-grain weight (i.e. seed
Plastochondria accumulate) it is metastable in the case of, it is however generally that pod grain number is more, and grain yield is higher.
At present, map based cloning and identify control soybean pod grain number and leaf Ln genes (Namhee Jeong,
Plant Cell, 2012；Chao Fang et al, Journal of Genetics and Genomics, 2013).Ln genes
By finely positioning in the 57.6kb DNA fragmentations section of No. 20 chromosome long arm of soybean, in the 1st extron of Ln genes
1 single base mutation by G → C is found in sequence, causes 1 amino acid variation (Asp → His), causes Four-seed pod ratio bright
Increased phenotypic variation is shown, maximum contribution rate is up to more than 20%.Mutated-genotype (corresponds to Four-seed pod labeled as genotype ln
Phenotype), and wild-type genotype is labeled as genotype Ln (corresponding to non-Four-seed pod phenotype).
Invention content
The technical problems to be solved by the invention are how to be quickly obtained the New soybean lines with merit.
In order to solve the above technical problems, present invention firstly provides a kind of molecular breeding method of New soybean lines, can wrap
Include following steps：
(1) using genotype be Ln and with specific trait I soybean varieties as receptor, using genotype for ln and have it is specific
The soybean varieties of character III are donor, by sexual hybridization, obtain hybridization F1Generation；
(2) in hybridization F1Soybean per plant of the genotype for ln is selected in generation, is then returned with the receptor, after being returned
For F1BC1；
(3) using the receptor as recurrent parent, with backcross progeny F1BC1Middle genotype is the soybean per plant continuous backcross of ln,
Obtain backcross progeny F1BCn；
(4) in backcross progeny F1BCnIt is middle to select genotype for ln and there is the soybean per plant of specific trait II, it is continuous to be selfed,
Until the self progeny F that the inheritance of acquired characters is stablizednBCn；
Self progeny FnBCnIn, genotype is ln and the strain with specific trait II is New soybean lines；It is described big
Beans new lines have merit；The merit can be (a) and/or (b) and/or (c)：(a) Four-seed pod ratio is significantly high
In receptor；(b) per mu yield is apparently higher than receptor；(c) 100-grain weight is not less than receptor.
In above-mentioned molecular breeding method, the specific trait I can be the character of non-Four-seed pod.The specific trait II can be
Four-seed pod ratio is apparently higher than receptor and 100-grain weight is not less than the character of receptor.The specific trait III can be that Four-seed pod ratio is bright
The aobvious character higher than receptor.
In above-mentioned molecular breeding method, the genotype is that the soybean varieties of Ln may be based on the gene of special SNP site
The type soybean varieties homozygous for GG.The genotype is that the soybean varieties of ln may be based on the gene of the special SNP site
The type soybean varieties homozygous for CC.The special SNP site can be sequence 1 in sequence table in soybean genome from 5 ' ends
74th nucleotide.
In above-mentioned molecular breeding method, in the step (1), the soybean varieties as receptor can be that state examines popularization soybean product
Kind.
In above-mentioned molecular breeding method, in the step (3), the continuous backcross was concretely returned for 2 generations.
It is described " using the receptor as recurrent parent, with backcross progeny F in above-mentioned molecular breeding method1BC1Middle genotype is
The soybean per plant continuous backcross of ln obtains backcross progeny F1BCn" concretely：
1. in backcross progeny F1BC1The middle F for selecting genotype as ln1BC1Then single plant is returned with the receptor, is returned
Hand over offspring F1BC2；
2. in backcross progeny F1BC2The middle F for selecting genotype as ln1BC2Then single plant is returned with the receptor, is returned
Hand over offspring F1BC3。
In above-mentioned molecular breeding method, in the step (4), the continuous selfing can be 3 generations of selfing.
It is described " in backcross progeny F in above-mentioned molecular breeding method1BCnIt is middle to select genotype for ln and with specific trait
II soybean per plant, it is continuous to be selfed, until the self progeny F that the inheritance of acquired characters is stablizednBCn" concretely：
1. in backcross progeny F1BC3It is middle to select genotype for ln and there is the soybean per plant of specific trait II, it is selfed, obtains
Self progeny F2BC3；
2. in self progeny F2BC3It is middle to select genotype for ln and there is the soybean per plant of specific trait II, it is selfed, obtains
Self progeny F3BC3；
3. in self progeny F3BC3It is middle to select genotype for ln and there is the soybean per plant of specific trait II, it is selfed, obtains
Self progeny F4BC3。
In above-mentioned molecular breeding method, the genotype be Ln and with specific trait I soybean varieties be middle yellow 13 or
Section's beans 1；The genotype is ln and the soybean varieties with specific trait III are rich 17 or black agriculture 40 of section.Specifically, in the middle
When Huang 13 is receptor, section rich 17 is used as donor；When section's beans 1 are receptor, black agriculture 40 is used as donor.
The present invention also protection uses the New soybean lines of any of the above-described method selection and breeding；The New soybean lines have
Merit；The merit can be (a) and/or (b) and/or (c)：(a) Four-seed pod ratio is apparently higher than receptor；(b) mu
Yield is apparently higher than receptor；(c) 100-grain weight is not less than receptor.
Application of any of the above-described method in soybean breeder also belongs to protection scope of the present invention.
In above application, the soybean breeder is using the New soybean lines of any of the above-described method selection and breeding as parent
This, is hybridized and/or is returned with other soybean varieties, obtain the soybean new product with other soybean varieties genetic backgrounds
System.
In above application, other soybean varieties can be that genotype is Ln and has the soybean product of the specific trait I
Kind.
It is demonstrated experimentally that using method provided by the invention utilize in Huang 13 and Ke Feng 17 select 4 New soybean lines (according to
It is secondary to be named as LZ904-1, KZ5-1, KZ5-5 and LZ904-7), select 2 New soybean lines using section's beans 1 and black agriculture 40
(being named as KH5-2 and KK4-4 successively).This 6 New soybean lines be high yielding soybeans new lines, especially KH5-2 and
Not only Comprehensive Traits are excellent, but also amount of increase in production is larger by LZ904-7：The per mu yield of KH5-2 is 202 kilograms, than receptor pair
According to No. 1 volume increase 6.88% of section's beans；The per mu yield of LZ904-7 is 201.6 kilograms, and Huang 13 increases production in being compareed than receptor
10.16%.A kind of molecular breeding method of high yielding soybeans new lines provided by the invention, it is short (only 3~4 years) with breeding cycle
With efficiency of selection it is high the advantages of.The present invention has important application value.
Description of the drawings
Fig. 1 is the technology path of the molecular breeding method of Huang 13 and Ke Feng, 17 breeding high-yield New soybean lines in utilizing.
Fig. 2 is the technology path of the molecular breeding method using section's beans 1 and black 40 breeding high-yield New soybean lines of agriculture.
Fig. 3 is the sequencing result for the genotype for detecting 6 New soybean lines.
Fig. 4 is the pod grain number distribution for detecting 6 New soybean lines, average 100-grain weight and per mu yield.
Specific embodiment
Following embodiment facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is conventional method unless otherwise specified.Test material used in following embodiments is certainly unless otherwise specified
What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even
Mean value.
Huang 13 is the product of Anhui Yong Minzhong industry Co., Ltd in soybean varieties.Hereinafter, it is yellow in soybean varieties
Huang 13 in 13 abbreviations.Middle yellow 13 promote mainly summer soybean varieties for Anhui Province North of Huai River area.
Soybean varieties section beans 1 (are recorded in following document：The sub- new soybean varieties of Li Mu " section's beans 1 " authorization passes through
Farmers' gives advice：Kind sparetime university sees, 2012 (2)：16-16) summer soybean varieties are promoted mainly for cities such as the Anhui Province Huaibei, Suzhou, Bozhous.Soybean
The black agriculture 40 of kind is recorded in following document：Perhaps No. 40, Chinese countrysides science and technology of the black agriculture of aobvious shore high quality soybean kinds, 1999 (12).
Hereinafter, No. 1 abbreviation section beans 1 of soybean varieties section beans, the referred to as black agriculture 40 of the black agriculture 40 of soybean varieties.
Rich No. 17 of soybean varieties section is by by soybean line by Inst. of Genetics and Development Biology, CAS
8511 form with the miscellaneous progress sexual hybridization selection and breeding of iron, pass through the Crops in Beijing variety certification committee within 2004 and authorize, authorization
Number is " beans 2004001 are examined in capital ".Hereinafter, rich No. 17 of rich No. 17 abbreviation sections of soybean varieties section.
Judge that the step of genotype of soybean is Ln or ln is as follows in following embodiments：
1st, soybean leaves 0.5g is taken, genomic DNA is then extracted using CTAB methods, obtains the genomic DNA of soybean.
2nd, using the genomic DNA of soybean as template, with 5 '-CCTGTGTTGTGATTTGCTGTTTA-3 ' and 5 '-
GCTTTTGTTGTTTCTTTGTTATTCA-3 ' carries out PCR amplification for primer, obtains pcr amplification product.
3rd, pcr amplification product is taken, is sequenced using DNA sequencer, analyze sequencing result and is made the following judgment：If
Genotype based on special SNP site is homozygous for CC, then the genotype of soybean is ln；If based on the base of special SNP site
Because type is homozygous for GG, then the genotype of soybean is Ln；Special SNP site is sequence 1 from 5 ' in sequence table in soybean genome
Play the 74th nucleotide in end.
Embodiment 1, using molecular breeding method breeding high-yield New soybean lines
Specific trait I：The character of non-Four-seed pod.
Specific trait II：Four-seed pod ratio is apparently higher than receptor kind and 100-grain weight is not less than the character of receptor kind.
Specific trait III：Four-seed pod ratio is apparently higher than the character of receptor kind.
Four-seed pod ratio accounts for the percentage of the total pod number of single plant for Four-seed pod number.
First, Huang 13 and Ke Feng 17 selects 4 New soybean lines in utilizing
The technology path of the molecular breeding method of Huang 13 and Ke Feng, 17 breeding high-yield New soybean lines is shown in Fig. 1 in utilization.
1st, Huang 13 is as receptor in selecting.Middle yellow 13 be that genotype is Ln and the state with specific trait I examines and promotes soybean
Kind.
2nd, section rich 17 is selected as donor.Section rich 17 is that genotype is ln and has the soybean varieties of specific trait III.
3rd, batch cross combination (30 or more) is prepared by section rich 17 (donor) and middle yellow 13 (receptors), each combines emasculation
At 40 or more, each cross combination harvests seed, as F to the number of spending of pollination1。
4th, the F that the genotype that step 3 obtains is ln is taken1Single plant is prepared batch backcrossing with middle yellow 13 and is combined (30 or more)
It is returned, each combination producing Crossbred spends number at 40 or more, and each backcrossing combination harvests seed, as F1BC1。
5th, the F that the genotype that step 4 obtains is ln is taken1BC1Single plant, with it is middle it is yellow 13 prepare batch backcrossing combine (30 with
On) be returned, each combination producing Crossbred spends number at 40 or more, and each backcrossing combination harvests seed, as F1BC2。
6th, the F that the genotype that step 5 obtains is ln is taken1BC2Single plant, with it is middle it is yellow 13 prepare batch backcrossing combine (30 with
On) be returned, each combination producing Crossbred spends number at 40 or more, and each backcrossing combination harvests seed, as F1BC3。
7th, it takes the genotype that step 6 obtains for ln and there is the F of specific trait II1BC3Single plant carries out individual plant selfing respectively,
Harvest seed, as F2BC3。
8th, it takes the genotype that step 7 obtains for ln and there is the F of specific trait II2BC3Strain carries out individual plant selfing respectively,
Harvest seed, as F3BC3。
9th, it takes the genotype that step 8 obtains for ln and there is the F of specific trait II3BC3Strain carries out individual plant selfing respectively,
Harvest seed, the F that as character inheritance is stablized4BC3。
In step 9,4 genotype are obtained altogether as ln and there are the New soybean lines of specific trait II, are named as successively
LZ904-1, KZ5-1, KZ5-5 and LZ904-7.
2nd, 2 New soybean lines are selected using section's beans 1 and black agriculture 40
Technology path using section's beans 1 and the molecular breeding method of black 40 breeding high-yield New soybean lines of agriculture is shown in Fig. 2.
1st, section's beans 1 are selected as receptor.Section's beans 1 be genotype be Ln and the state with specific trait I examine it is excellent big
Beans kind.
2nd, black agriculture 40 is selected as donor.Black agriculture 40 is that genotype is ln and has the soybean varieties of specific trait III.
3rd, black agriculture 40 (donor) and section's beans 1 (receptor) are prepared into batch cross combination (30 or more), each combination is gone
At 40 or more, each cross combination harvests seed, as F to the number of spending of hero pollination1。
4th, the F that the genotype that step 3 obtains is ln is taken1Single plant is prepared batch backcrossing with section beans 1 and is combined (30 or more)
It is returned, each combination producing Crossbred spends number at 40 or more, and each backcrossing combination harvests seed, as F1BC1。
5th, the F that the genotype that step 4 obtains is ln is taken1BC1Single plant, with section beans 1 prepare batch backcrossing combine (30 with
On) be returned, each combination producing Crossbred spends number at 40 or more, and each backcrossing combination harvests seed, as F1BC2。
6th, the F that the genotype that step 5 obtains is ln is taken1BC2Single plant, with section beans 1 prepare batch backcrossing combine (30 with
On) be returned, each combination producing Crossbred spends number at 40 or more, and each backcrossing combination harvests seed, as F1BC3。
7th, it takes the genotype that step 6 obtains for ln and there is the F of specific trait II1BC3Single plant carries out individual plant selfing respectively,
Harvest seed, as F2BC3。
8th, it takes the genotype that step 7 obtains for ln and there is the F of specific trait II2BC3Strain carries out individual plant selfing respectively,
Harvest seed, as F3BC3。
9th, it takes the genotype that step 8 obtains for ln and there is the F of specific trait II3BC3Strain carries out individual plant selfing respectively,
Harvest seed, the F that as character inheritance is stablized4BC3。
In step 9,2 genotype are obtained altogether as ln and there are the New soybean lines of specific trait II, are named as successively
KH5-2 and KK4-4.
3rd, the genotype of 2 New soybean lines that 4 New soybean lines and step 2 that detecting step one obtains obtain
Soybean to be measured is LZ904-1, KZ5-1, KZ5-5, LZ904-7, KH5-2, KK4-4, middle yellow 13, section are rich 17, section's beans 1
Number or black agriculture 40.
1st, soybean leaves 0.5g to be measured is taken, genomic DNA is then extracted using CTAB methods, obtains the genome of soybean to be measured
DNA。
2nd, using the genomic DNA of soybean to be measured as template, with 5 '-CCTGTGTTGTGATTTGCTGTTTA-3 ' and 5 '-
GCTTTTGTTGTTTCTTTGTTATTCA-3 ' carries out PCR amplification for primer, obtains pcr amplification product.
3rd, pcr amplification product is taken, is sequenced using DNA sequencer, analyze sequencing result and is made the following judgment：If
Genotype of the soybean to be measured based on special SNP site is homozygous for CC, then the genotype of soybean to be measured is ln；If soybean to be measured
Genotype based on the special SNP site is homozygous for GG, then the genotype of soybean to be measured is Ln；The special SNP site
For sequence 1 in sequence table in soybean genome from 5 ' ends the 74th nucleotide.
Part sequencing result see Fig. 3 (1 be middle yellow 13,2 is section's beans 1, and 3 be LZ904-1, and 4 be KZ5-1, and 5 be KZ5-5,6
For LZ904-7,7 be KH5-2, and 8 be KK4-4, and 9 be section rich 17,10 be black agriculture 40).Sequencing result shows two receptor kinds
The genotype of (Huang 13 and section's beans 1 in i.e.) is Ln, and two donor kinds (i.e. section rich 17 and Hei Nong 40), step 1 obtain
The genotype of 2 New soybean lines that 4 New soybean lines and step 2 obtain is ln.
4th, the pod grain number point for 2 New soybean lines that 4 New soybean lines and step 2 that detecting step one obtains obtain
Cloth, average 100-grain weight and per mu yield
Soybean to be measured is LZ904-1, KZ5-1, KZ5-5, LZ904-7, KH5-2, KK4-4, middle yellow 13, section are rich 17, section's beans 1
Number or black agriculture 40.
Take soya seeds to be measured, plant 3 different tests bases in Anhui and Henan, then count the distribution of pod grain number,
Average 100-grain weight and per mu yield.The distribution of pod grain number is specially that (one seeded pod number accounts for the percentage of the total pod number of single plant to one seeded pod ratio
Than), two pod ratios (two pod numbers account for the percentage of the total pod number of single plant), (three pod numbers account for the total pod number of single plant for three pod ratios
Percentage) and Four-seed pod ratio (Four-seed pod number accounts for the percentage of the total pod number of single plant).
Experimental result is shown in Fig. 4 (A is distributed for pod grain number, and B is average 100-grain weight, and C is per mu yield).The result shows that with middle Huang
13 compare, and the Four-seed pod ratio and per mu yield of 4 New soybean lines are significantly increased, and (Four-seed pod ratio middle yellow 13 increases
16.8%~23.8%), average 100-grain weight is without being substantially reduced；Compared with section's beans 1, the Four-seed pod ratio of two New soybean lines
Rate and per mu yield are significantly increased (Four-seed pod ratio increases by 15.5% and 18.8% for No. 1 compared with section's beans), and average 100-grain weight
Nothing is substantially reduced.It follows that 2 New soybean lines that 4 New soybean lines and step 2 that step 1 obtains obtain are
High yielding soybeans new lines.Especially KH5-2 and LZ904-7 are (although show that LZ904-1 is averaged 100-grain weight and per mu yield in Fig. 4 B and C
Measure highest, but because the strain infect soybean Mosaic due to be eliminated), not only Comprehensive Traits are excellent, and amount of increase in production compared with
Greatly：The per mu yield of KH5-2 is 202 kilograms, than No. 1 volume increase 6.88% of receptor control section beans；The per mu yield of LZ904-7
It is 201.6 kilograms, 13 volume increase 10.16% of Huang in being compareed than receptor.
<110>Inst. of Genetics and Development Biology, CAS
<120>A kind of molecular breeding method of high yielding soybeans new lines
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 186
<212> DNA
<213>Soybean（Glycine max（L.）Merr.）
<220>
<221> misc_feature
<222>（74）
<223> n is c or g
<400> 1
ctctaaaccc tttaactttc tctctcttat gactttgttg ttcctttaca ggagaccaga 60
acgaaacccc ttanatctta acaatttgcc cgatgagtac tctagagatg gcaaacaagt 120
cctcgaagac catacctctt catccggtaa acttcatgat cataccaata tatatatata 180
tgcacg 186
Claims (10)
1. a kind of molecular breeding method of New soybean lines, includes the following steps：
(1) using genotype be Ln and with specific trait I soybean varieties as receptor, using genotype for ln and have specific trait
III soybean varieties are donor, by sexual hybridization, obtain hybridization F1Generation；
(2) in hybridization F1Soybean per plant of the genotype for ln is selected in generation, is then returned with the receptor, obtains backcross progeny
F1BC1；
(3) using the receptor as recurrent parent, with backcross progeny F1BC1Middle genotype is the soybean per plant continuous backcross of ln, is obtained
Backcross progeny F1BCn；
(4) in backcross progeny F1BCnIt is middle to select genotype for ln and there is the soybean per plant of specific trait II, it is continuous to be selfed, until
The self progeny F that the inheritance of acquired characters is stablizednBCn；
Self progeny FnBCnIn, genotype is ln and the strain with specific trait II is New soybean lines；The soybean is new
Strain has merit；The merit is (a) and/or (b) and/or (c)：(a) Four-seed pod ratio is apparently higher than receptor；
(b) per mu yield is apparently higher than receptor；(c) 100-grain weight is not less than receptor；
The specific trait I is the character of non-Four-seed pod；
The specific trait II is apparently higher than receptor for Four-seed pod ratio and 100-grain weight is not less than the character of receptor；
The specific trait III is apparently higher than the character of receptor for Four-seed pod ratio；
The soybean varieties that the genotype is Ln are the homozygous soybean varieties of GG for the genotype based on special SNP site；
The soybean varieties that the genotype is ln are the homozygous soybean product of CC for the genotype based on the special SNP site
Kind；
The special SNP site is the 74th nucleotide from 5 ' ends of sequence 1 in sequence table in soybean genome.
2. the method as described in claim 1, it is characterised in that：In the step (3), the continuous backcross is 2 generations of backcrossing.
3. method as claimed in claim 1 or 2, it is characterised in that：It is described " after using the receptor as recurrent parent, with backcrossing
For F1BC1Middle genotype is the soybean per plant continuous backcross of ln, obtains backcross progeny F1BCn" be：
1. in backcross progeny F1BC1The middle F for selecting genotype as ln1BC1Then single plant is returned with the receptor, after being returned
For F1BC2；
2. in backcross progeny F1BC2The middle F for selecting genotype as ln1BC2Then single plant is returned with the receptor, after being returned
For F1BC3。
4. the method as described in claims 1 to 3 is any, it is characterised in that：In the step (4), the continuous selfing is certainly
Handed over for 3 generations.
5. the method as described in Claims 1-4 is any, it is characterised in that：It is described " in backcross progeny F1BCnMiddle selection genotype
It is continuous to be selfed for ln and the soybean per plant with specific trait II, until the self progeny F that the inheritance of acquired characters is stablizednBCn”
For：
1. in backcross progeny F1BC3Middle to select genotype for ln and have the soybean per plant of specific trait II, selfing is selfed
Offspring F2BC3；
2. in self progeny F2BC3Middle to select genotype for ln and have the soybean per plant of specific trait II, selfing is selfed
Offspring F3BC3；
3. in self progeny F3BC3Middle to select genotype for ln and have the soybean per plant of specific trait II, selfing is selfed
Offspring F4BC3。
6. the method as described in claim 1 to 5 is any, it is characterised in that：The genotype is Ln and has specific trait I
Soybean varieties are middle yellow 13 or section's beans 1；The genotype be ln and with specific trait III soybean varieties be section rich 17 or
Black agriculture 40.
7. using the New soybean lines of any method selection and breeding of claim 1 to 6；The New soybean lines have Optimality
Shape；The merit is (a) and/or (b) and/or (c)：(a) Four-seed pod ratio is apparently higher than receptor；(b) per mu yield is apparent
Higher than receptor；(c) 100-grain weight is not less than receptor.
8. application of any method of claim 1 to 6 in soybean breeder.
9. application as claimed in claim 8, it is characterised in that：The soybean breeder is that claim 1 to 6 is any described
The New soybean lines of method selection and breeding are hybridized and/or are returned with other soybean varieties, obtain having described other as parent
The New soybean lines of soybean varieties genetic background.
10. application as claimed in claim 9, it is characterised in that：Other soybean varieties are that genotype is Ln and with spy
The soybean varieties of qualitative shape I.
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| CN109628631A (en) * | 2019-01-25 | 2019-04-16 | 中国农业科学院作物科学研究所 | Nest-type PRC specific primer is to, kit and preparation method thereof and the application in auxiliary identification Ln gene editing material |
| CN113785771A (en) * | 2021-09-28 | 2021-12-14 | 吉林省农业科学院 | Method for introducing annual wild soybean chromosome translocation fragments into cultivated soybeans |
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| CN109628631A (en) * | 2019-01-25 | 2019-04-16 | 中国农业科学院作物科学研究所 | Nest-type PRC specific primer is to, kit and preparation method thereof and the application in auxiliary identification Ln gene editing material |
| CN113785771A (en) * | 2021-09-28 | 2021-12-14 | 吉林省农业科学院 | Method for introducing annual wild soybean chromosome translocation fragments into cultivated soybeans |
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| CN108260522B (en) | 2020-08-11 |
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