CN108254345A - A kind of method that roxithromycin is measured based on phloxine - Google Patents

A kind of method that roxithromycin is measured based on phloxine Download PDF

Info

Publication number
CN108254345A
CN108254345A CN201810004921.3A CN201810004921A CN108254345A CN 108254345 A CN108254345 A CN 108254345A CN 201810004921 A CN201810004921 A CN 201810004921A CN 108254345 A CN108254345 A CN 108254345A
Authority
CN
China
Prior art keywords
roxithromycin
scattering strength
concentration
added
ethyl alcohol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810004921.3A
Other languages
Chinese (zh)
Inventor
唐宁莉
范晶晶
马小龙
王文平
师蓉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guilin University of Technology
Original Assignee
Guilin University of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guilin University of Technology filed Critical Guilin University of Technology
Priority to CN201810004921.3A priority Critical patent/CN108254345A/en
Publication of CN108254345A publication Critical patent/CN108254345A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices

Abstract

The invention discloses a kind of methods that roxithromycin is measured based on phloxine.In 8 colorimetric cylinders, be separately added into 0.0,0.05,0.1,0.5,1.0,1.5,2.0,2.5mL 0.05mg/L roxithromycin, it is separately added into phloxine and acetic acid, it is settled to 10 mL, after reaction, with 1cm cuvettes on fluophotometer in a manner of λ ex=λ em scanning optical spectrum, measure the scattering strength I and I added with roxithromycin and the blank for being not added with roxithromycin respectively at 327 nm0, calculate scattering strength difference △ I=I I0.Roxithromycin capsules is taken to be dissolved with absolute ethyl alcohol, is filtered, is settled to 100mL, 5mL is taken to be settled to 50mL with ethyl alcohol, 0.5mL ethyl alcohol is taken to be settled to 50mL as prepare liquid, scattering strength value is measured in the same method, calculates the content of roxithromycin in capsule.The method of the present invention is easy, high sensitivity.

Description

A kind of method that roxithromycin is measured based on phloxine
Technical field
The present invention relates to the assay methods of roxithromycin, are based particularly on the resonance scattering that phloxine measures roxithromycin Spectroscopic methodology.
Background technology
Roxithromycin (ROX) is at present clinically using the excellent novel erythromycin product of extensive, curative effect, it is to golden yellow Portugal Grape coccus, staphylococcus epidermis, streptococcus pneumonia, enterococcus, anaerobic bacteria, mycoplasma pneumoniae, Chlamydia, gonococcus etc. have anti- Bacterium acts on, and can reach and thoroughly treats effect uneasy to recur.The method for measuring roxithromycin content at present mainly has biological standardization Method, spectrophotometry and chromatography etc..Resonance scattering spectrometry has the characteristics that easy quick, high sensitivity, detection limit are low, closely Trace analysis is had been used for over year, but by the use of phloxine as probe, the method that roxithromycin is measured using resonance scattering technology There is not been reported.This patent reacts to form ionic associate in an acidic solution using roxithromycin and phloxine, leads Cause system resonance scattering intensity significantly increases, and it is good to establish a kind of selectivity accordingly, and easy, accurate, sensitive to measure Luo Hong mould The resonance scattering spectroscopy new method of element.
Invention content
The object of the present invention is to provide the resonance scattering spectrometries that a kind of phloxine measures roxithromycin.
The specific steps are：
In the colorimetric cylinder of 8 10mL, be separately added into 0.0,0.05,0.1,0.5,1.0,1.5,2.0,2.5mL it is a concentration of The roxithromycin of 0.05mg/L, then be separately added into 8 colorimetric cylinders a concentration of 0.001mol/L of 1.0mL phloxine and The acetic acid of a concentration of 0.02mol/L of 2.0mL is settled to 10mL with ultra-pure water, is reacted at 45 DEG C after ten minutes, with 1cm cuvettes In on fluophotometer in a manner of λ ex=λ em scanning optical spectrum, measured respectively at 327nm added with roxithromycin solution dissipate It penetrates intensity I and is not added with the scattering strength I of the blank solution of roxithromycin0, calculate the difference △ I=I-I of scattering strength0, scattering Strength difference △ I are with roxithromycin concentration c 2.5 × 10-4~1.25 × 10-2In good linear relationship, line in the range of mg/L Property regression equation is：△ I=2.586 × 104C+35.13, correlation coefficient r=0.9971, detection are limited to 1.82 × 10-4mg/L。
Roxithromycin capsules 1 separately are taken, weigh 0.0050g, are dissolved with 20mL absolute ethyl alcohols, filtering, filtrate ultra-pure water Then constant volume takes the ethyl alcohol constant volume that the above-mentioned solution mass percent concentrations of 5mL are 20% to hold in 50mL in 100mL volumetric flasks In measuring bottle, then the ethyl alcohol constant volume that the solution 0.5mL mass percent concentrations after dilution is taken to be 20% is made in 50mL volumetric flasks For prepare liquid, 0.7mL prepare liquids is taken to be measured in the same method scattering strength value, calculate the content of roxithromycin in roxithromycin capsules.
Assay method of the present invention is fast and simple, high sensitivity.
Description of the drawings
Fig. 1 is blank of the embodiment of the present invention and 5.0 × 10-3The resonance light scattering spectrogram of mg/L roxithromycin.
It is marked in figure：a：Blank；b：Blank -5.0 × 10-3mg/L ROX。
Specific embodiment
Embodiment：
In the colorimetric cylinder of 8 10mL, be separately added into 0.0,0.05,0.1,0.5,1.0,1.5,2.0,2.5mL it is a concentration of The roxithromycin of 0.05mg/L, then be separately added into 8 colorimetric cylinders a concentration of 0.001mol/L of 1.0mL phloxine and The acetic acid of a concentration of 0.02mol/L of 2.0mL, 10mL is settled to ultra-pure water, it is reacted at 45 DEG C after ten minutes, with 1cm cuvettes In on fluophotometer in a manner of λ ex=λ em scanning optical spectrum, measured respectively at 327nm added with roxithromycin solution dissipate It penetrates intensity I and is not added with the scattering strength I of the blank solution of roxithromycin0, calculate the difference △ I=I-I of scattering strength0, scattering Strength difference △ I are with roxithromycin concentration c 2.5 × 10-4~1.25 × 10-2In good linear relationship, line in the range of mg/L Property regression equation is：△ I=2.586 × 104C+35.13, correlation coefficient r=0.9971, detection are limited to 1.82 × 10-4mg/L。 Take what the peaceful phoenix Pharmacy stock Co., Ltd (sample 1) in Zhuhai and Jiangsu Fubang .Pharm Co., Ltd.'s (sample 2) produced respectively Each (specification of roxithromycin capsules：150mg/), 0.0050g samples are weighed respectively in beaker, with 20mL absolute ethyl alcohols Dissolving, filtering, filtrate in 100mL volumetric flasks, then take the above-mentioned solution mass percents of 5mL dense respectively with ultra-pure water constant volume Spend the ethyl alcohol constant volume for 20% in 50mL volumetric flasks, then respectively take dilution after solution 0.5mL be with mass percent concentration 20% ethyl alcohol constant volume, as prepare liquid, takes 0.7mL prepare liquids to be measured in the same method scattering strength value respectively in 50mL volumetric flasks, point The content of roxithromycin in roxithromycin capsules is not calculated, the results are shown in Table 1.
Table 1：The measurement result (n=5) of roxithromycin content in roxithromycin capsules

Claims (1)

  1. A kind of 1. method for measuring roxithromycin, it is characterised in that the specific steps are：
    (1) in the colorimetric cylinder of 8 10 mL, be separately added into 0.0,0.05,0.1,0.5,1.0,1.5,2.0,2.5mL it is a concentration of The roxithromycin of 0.05mg/L, then be separately added into 8 colorimetric cylinders a concentration of 0.001mol/L of 1.0 mL phloxine and The acetic acid of a concentration of 0.02mol/L of 2.0mL is settled to 10 mL, after being reacted 10 minutes at 45 DEG C, with 1cm ratios with ultra-pure water Color ware on fluophotometer in a manner of λ ex=λ em scanning optical spectrum, measured respectively added with roxithromycin solution at 327 nm Scattering strength I and be not added with roxithromycin blank solution scattering strength I0, calculate difference △ I=I-I of scattering strength0, Its scattering strength difference △ I are with roxithromycin concentration c 2.5 × 10-4~1.25 × 10-2In good linear in the range of mg/L Relationship, equation of linear regression are：△I=2.586×104C+35.13, correlation coefficient r=0.9971, detection are limited to 1.82 × 10- 4mg/L；
    (2) roxithromycin capsules 1 separately are taken, weighs 0.0050, dissolved with 20mL absolute ethyl alcohols, filtering, filtrate is determined with ultra-pure water It is dissolved in 100mL volumetric flasks, then takes the ethyl alcohol constant volume that the above-mentioned solution mass percent concentrations of 5mL are 20% in 50mL capacity In bottle, then take the solution 0.5mL after dilution by the use of the ethyl alcohol constant volume that mass percent concentration is 20% in 50mL volumetric flasks as treating Liquid is surveyed, takes 0.7mL prepare liquids according to step（1）Method measure scattering strength value, calculate roxithromycin in roxithromycin capsules Content amount.
CN201810004921.3A 2018-01-03 2018-01-03 A kind of method that roxithromycin is measured based on phloxine Pending CN108254345A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810004921.3A CN108254345A (en) 2018-01-03 2018-01-03 A kind of method that roxithromycin is measured based on phloxine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810004921.3A CN108254345A (en) 2018-01-03 2018-01-03 A kind of method that roxithromycin is measured based on phloxine

Publications (1)

Publication Number Publication Date
CN108254345A true CN108254345A (en) 2018-07-06

Family

ID=62725675

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810004921.3A Pending CN108254345A (en) 2018-01-03 2018-01-03 A kind of method that roxithromycin is measured based on phloxine

Country Status (1)

Country Link
CN (1) CN108254345A (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102095697A (en) * 2010-12-07 2011-06-15 桂林理工大学 Method for measuring roxithromycin

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102095697A (en) * 2010-12-07 2011-06-15 桂林理工大学 Method for measuring roxithromycin

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
M.I.WALSH ET AL.: "Spectrophotometric Determination of Four Macrolide Antibiotics in Pharmaceutical Formulations and Biological Fluids via Binary Complex Formation with Eosin and Spectrophotometry", 《JOURNAL OF AOAC INTERNATIONAL》 *
周尚 等: "卤代荧光素与西布曲明相互作用的共振散射光谱特征及其分析应用", 《分析测试学报》 *
杨清玲 等: "Ag+-Cl—多取代荧光素体系的RRS和RNLS光谱及其分析", 《中国科学:化学》 *
陈莲惠 等: "对二甲基氨基偶氮苯染料共振瑞利散射法测定红霉素", 《分析试验室》 *

Similar Documents

Publication Publication Date Title
Omar et al. Validated spectrofluorimetric method for determination of selected aminoglycosides
US9068996B2 (en) Analyte detection method
CN101819137A (en) Urinary iodine tester and analysis method thereof
CN110609027A (en) Method for rapidly detecting chlorpromazine hydrochloride in feed
CN101093214B (en) Method for measuring density of anti-epileptic in blood
CN201449370U (en) Multi-wavelength nucleic acid protein chromatographic separation detection device
CN102507482A (en) Detection method and reagents for quantitatively detecting 6-methyl-2-thiopyridyl-N-acetyl-beta-D-glucosaminide (MPT-NAG)
CN102435587A (en) Method for rapidly determining nitrite in water through nanogold resonance scattering spectrometry
CN108254345A (en) A kind of method that roxithromycin is measured based on phloxine
CN104698093B (en) Polyol method for quick based on capillary siphoning effect Yu phenyl boric acid recognition principle
Zhang et al. An asynchronous-alternating merging-zone flow-injection gold nanoparticles probe method for determination of anti-diabetic pioglitazone hydrochloride medicine
RU2424515C2 (en) Method of determining cinnarizine
CN202305395U (en) Device for quickly testing optical activity
CN106370646B (en) Method for detecting protein in sample to be detected
RU2391659C1 (en) Method of detecting silver using polymethacrylate matrix
CN1261749C (en) Method for rapidly and quantitatively determining the triterpenoid content in ganoderma lucidum
CN108318456A (en) A method of roxithromycin is measured based on eosin
Chen et al. Label-free fluorescence turn on detection of tiopronin with tunable dynamic range based on the ensemble of Alizarin Red S/copper ion
RU2428686C1 (en) Method of determining cobalt (ii) using polymethacrylate matrix
CN108254344A (en) A kind of method that melamine is measured using crystal violet
Vuković et al. One-step solid-phase UV spectrophotometric method for phenol determination in vaccines: Development and quality assessment
CN102645422A (en) Method for measuring pipemidic acid by using resonance scattering spectrum
Ma et al. Resonance Rayleigh scattering methods for the determination of chitosan with Congo red as probe
RU2517489C1 (en) Method for picamilon measurement
CN111122483A (en) Method for measuring content of chlorphenesin by ultraviolet spectrophotometry

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20180706