CN108254286A - A kind of zooplankter sample biomass estimation method - Google Patents
A kind of zooplankter sample biomass estimation method Download PDFInfo
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- CN108254286A CN108254286A CN201711321704.9A CN201711321704A CN108254286A CN 108254286 A CN108254286 A CN 108254286A CN 201711321704 A CN201711321704 A CN 201711321704A CN 108254286 A CN108254286 A CN 108254286A
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- zooplankter
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N5/00—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid
- G01N5/04—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid by removing a component, e.g. by evaporation, and weighing the remainder
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Abstract
The invention discloses a kind of zooplankter sample biomass estimation methods, include the following steps：(a) sample is acquired, and measures population of samples product V；(b) after sample precipitates 48 hours in cylindrical separatory funnel, supernatant liquor is drawn, the sample after precipitation concentration is put into reagent bottle, weighing obtains sample total weight M1；(c) bolting silk is cut into big with Buchner funnel etc., soaks and be taped against in funnel, after the excessive moisture that Buchner funnel bolting silk is pumped with vacuum pump, transfer the sample on bolting silk, moisture is pumped with vacuum pump, obtain level-one filtrate；(d) buret is taken, the tubular specimen container that one is made of brass frame and etting is put into the buret, the liquid in most sample container is first got rid of after water filling into the sample container, level-one filtrate is put into the sample container again, throw away the liquid in sample container, two level filtrate is obtained, weighing obtains two level filtrate weight M2；(e) biomass of zooplankter is in sample（M1‑M2）/V.
Description
Technical field
The present invention relates to zooplankton biomass determination techniques field more particularly to a kind of zooplankter sample biometric measurements
Determine method.
Background technology
Biomass refers to the weight of the organism in the presence of unit volume.Zooplankter sample biomass in the prior art
Measure generally comprise the steps：After sample sedimentation a period of time, supernatant is siphoned away, by the Sample storage after concentration to small
In bottle；Bolting silk is cut into big with Buchner funnel etc., soaks and be taped against in funnel, after pumping excessive moisture with vacuum pump, weigh bolting silk
Weight；It transfers the sample on the bolting silk of Buchner funnel, excessive moisture is pumped with vacuum pump；Sample is transferred to together with bolting silk
On filter paper, further suck dry moisture is put into scales/electronic balance weighing；Example weight=total weight-bolting silk weight.This assay method
It is disadvantageous in that：It has part zooplankter attachment on a buchner funnel or through bolting silk to enter in filtrate, result in
The example weight measured is less than practical example weight.
Invention content
The present invention provides a kind of zooplankter sample biomass estimation side to overcome above-mentioned deficiency of the prior art
Method, can in measure water body zooplankter biomass.
To achieve these goals, the present invention uses following technical scheme：
A kind of zooplankter sample biomass estimation method, includes the following steps：(a) it samples：According to the Form division of water body not
Same region, the then share according to shared by different zones, in proportion water sampling mixing then take out a part and are used as sample,
Measure population of samples product V；(b) concentrating sample：After sample precipitates 48 hours in cylindrical separatory funnel, supernatant liquor is drawn,
Sample after precipitation concentration is put into reagent bottle, and weighing obtains sample total weight M1；(c) sample by-pass filtration：Bolting silk is cut into
It is big with Buchner funnel etc., it soaks and is taped against in funnel, after the excessive moisture that Buchner funnel bolting silk is pumped with vacuum pump, sample is shifted
Onto bolting silk, moisture is pumped with vacuum pump, obtains level-one filtrate；(d) sample secondary filtration：The a diameter of 1.5cm, length is taken to be
The buret of 20cm, the tubular specimen container that one is made of brass frame and etting is put into the buret, first to the sample
The liquid in most sample container is got rid of in product container after water filling, then level-one filtrate is put into the sample container, throws away sample container
Interior liquid, obtains two level filtrate, and weighing obtains two level filtrate weight M2；(e) biomass calculates：The life of zooplankter in sample
Object amount is（M1-M2）/V.
As a preferred technical solution of the present invention, in step (a), the method sampled in a region is：According to
Water depth takes equivalent water sample to be mixed every 0.5 meter or 1 meter or 2 meters.
As a preferred technical solution of the present invention, in step (b), the sample size of precipitation concentration is 30 or 50 milliliters.
As a preferred technical solution of the present invention, in step (d), the aperture of the etting is 100 ~ 150 microns.
The advantageous effect that is reached of the present invention is compared with prior art：It being capable of measure water the present invention provides one kind
The assay method of the biomass of zooplankter in body.
Specific embodiment
The preferred embodiment of the present invention is illustrated below, it should be understood that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
A kind of zooplankter sample biomass estimation method, includes the following steps：
(a) it samples：According to the different region of the Form division of water body, in a region according to water depth, every 0.5 meter or
1 meter or 2 meters takes equivalent water sample to be mixed, then the share according to shared by different zones, and water sampling mixes in proportion, Ran Houqu
Go out a part as sample, measure population of samples product V；
(b) concentrating sample：After sample precipitates 48 hours in cylindrical separatory funnel, supernatant liquor is drawn, after precipitation concentration
Sample is put into reagent bottle, and the sample size of precipitation concentration is 30 or 50 milliliters, and weighing obtains sample total weight M1；
(c) sample by-pass filtration：Bolting silk is cut into big with Buchner funnel etc., soaks and be taped against in funnel, Bu Shi is pumped with vacuum pump
It after the excessive moisture of funnel bolting silk, transfers the sample on bolting silk, moisture is pumped with vacuum pump, obtain level-one filtrate；
(d) sample secondary filtration：It takes an a diameter of 1.5cm, the buret that length is 20cm, is by brass frame and aperture by one
The tubular specimen container of 112 microns of etting composition is put into the buret, first gets rid of most sample after water filling into the sample container
Liquid in product container, then level-one filtrate is put into the sample container, the liquid in sample container is thrown away, obtains two level filter
Liquid, weighing obtain two level filtrate weight M2；
(e) biomass calculates：The biomass of zooplankter is in sample（M1-M2）/V.
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify to the technical solution recorded in foregoing embodiments or carry out equivalent replacement to which part technical characteristic.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in the present invention's
Within protection domain.
Claims (4)
1. a kind of zooplankter sample biomass estimation method, includes the following steps：
(a) it samples：According to the different region of the Form division of water body, then the share according to shared by different zones, takes in proportion
Water sample mixes, and then takes out a part as sample, measures population of samples product V；
(b) concentrating sample：After sample precipitates 48 hours in cylindrical separatory funnel, supernatant liquor is drawn, after precipitation concentration
Sample is put into reagent bottle, and weighing obtains sample total weight M1；
(c) sample by-pass filtration：Bolting silk is cut into big with Buchner funnel etc., soaks and be taped against in funnel, Bu Shi is pumped with vacuum pump
It after the excessive moisture of funnel bolting silk, transfers the sample on bolting silk, moisture is pumped with vacuum pump, obtain level-one filtrate；
(d) sample secondary filtration：An a diameter of 1.5cm is taken, the buret that length is 20cm, by one by brass frame and etting group
Into tubular specimen container be put into the buret, the liquid in most sample container is first got rid of after water filling into the sample container,
Level-one filtrate is put into the sample container again, throws away the liquid in sample container, obtains two level filtrate, weighing obtains two level filter
Liquid weight M2；
(e) biomass calculates：The biomass of zooplankter is in sample（M1-M2）/V.
2. a kind of zooplankter sample biomass estimation method according to claim 1, which is characterized in that in step (a),
The method sampled in a region is：According to water depth, equivalent water sample is taken to be mixed every 0.5 meter or 1 meter or 2 meters.
3. a kind of zooplankter sample biomass estimation method according to claim 1, which is characterized in that in step (b),
The sample size of precipitation concentration is 30 or 50 milliliters.
4. a kind of zooplankter sample biomass estimation method according to claim 1, which is characterized in that in step (d),
The aperture of the etting is 100 ~ 150 microns.
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| CN201711321704.9A CN108254286A (en) | 2017-12-12 | 2017-12-12 | A kind of zooplankter sample biomass estimation method |
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| CN201711321704.9A CN108254286A (en) | 2017-12-12 | 2017-12-12 | A kind of zooplankter sample biomass estimation method |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1308302A1 (en) * | 1985-12-09 | 1987-05-07 | Всесоюзное Научно-Производственное Объединение По Рыбоводству | Method of determining biomass of zooplankton |
| CN105136552A (en) * | 2015-10-15 | 2015-12-09 | 北京农学院 | Device and method for extracting apoplast juice from plant fruit tissue |
| CN205832769U (en) * | 2016-07-19 | 2016-12-28 | 浙江海洋大学 | A kind of zooplankton classified filtering device |
| CN206069861U (en) * | 2016-07-11 | 2017-04-05 | 四川农业大学 | A kind of centrifuge tube for separating plant intercellular washing fluids |
| CN106769380A (en) * | 2016-12-30 | 2017-05-31 | 浙江省海洋水产研究所 | A kind of phytoplankton scene separation and concentration method |
| CN107367401A (en) * | 2017-08-03 | 2017-11-21 | 中国林业科学研究院林业新技术研究所 | A kind of plankton collecting device and acquisition method |
-
2017
- 2017-12-12 CN CN201711321704.9A patent/CN108254286A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1308302A1 (en) * | 1985-12-09 | 1987-05-07 | Всесоюзное Научно-Производственное Объединение По Рыбоводству | Method of determining biomass of zooplankton |
| CN105136552A (en) * | 2015-10-15 | 2015-12-09 | 北京农学院 | Device and method for extracting apoplast juice from plant fruit tissue |
| CN206069861U (en) * | 2016-07-11 | 2017-04-05 | 四川农业大学 | A kind of centrifuge tube for separating plant intercellular washing fluids |
| CN205832769U (en) * | 2016-07-19 | 2016-12-28 | 浙江海洋大学 | A kind of zooplankton classified filtering device |
| CN106769380A (en) * | 2016-12-30 | 2017-05-31 | 浙江省海洋水产研究所 | A kind of phytoplankton scene separation and concentration method |
| CN107367401A (en) * | 2017-08-03 | 2017-11-21 | 中国林业科学研究院林业新技术研究所 | A kind of plankton collecting device and acquisition method |
Non-Patent Citations (3)
| Title |
|---|
| 中国环境监测总站: "《近岸海域环境监测技术》", 30 October 2013, 中国环境出版社 * |
| 柯志新 等: "大亚湾冬季不同粒级浮游生物的氮稳定同位素特征及其与生物量的关系", 《生态学报》 * |
| 赵文: "《水生生物学》", 31 August 2005, 中国农业出版社 * |
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Application publication date: 20180706 |