CN107102052B - Based on the uric acid electrochemical sensor containing active copper carbon dots and its application - Google Patents
Based on the uric acid electrochemical sensor containing active copper carbon dots and its application Download PDFInfo
- Publication number
- CN107102052B CN107102052B CN201710279857.5A CN201710279857A CN107102052B CN 107102052 B CN107102052 B CN 107102052B CN 201710279857 A CN201710279857 A CN 201710279857A CN 107102052 B CN107102052 B CN 107102052B
- Authority
- CN
- China
- Prior art keywords
- electrode
- carbon dots
- active copper
- uric acid
- containing active
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 title claims abstract description 39
- 229940116269 uric acid Drugs 0.000 title claims abstract description 39
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 title claims abstract description 38
- AHADSRNLHOHMQK-UHFFFAOYSA-N methylidenecopper Chemical compound [Cu].[C] AHADSRNLHOHMQK-UHFFFAOYSA-N 0.000 title claims abstract description 29
- 239000010949 copper Substances 0.000 claims abstract description 19
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 13
- 238000006243 chemical reaction Methods 0.000 claims abstract description 10
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229920000642 polymer Polymers 0.000 claims abstract description 7
- 238000001027 hydrothermal synthesis Methods 0.000 claims abstract description 6
- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical class Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 claims abstract description 5
- 238000004070 electrodeposition Methods 0.000 claims abstract description 5
- 229910052697 platinum Inorganic materials 0.000 claims abstract description 5
- 230000008569 process Effects 0.000 claims abstract description 5
- 150000003839 salts Chemical class 0.000 claims abstract description 5
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims abstract description 3
- 238000005119 centrifugation Methods 0.000 claims abstract 2
- 239000000243 solution Substances 0.000 claims description 24
- 238000002360 preparation method Methods 0.000 claims description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 229910021592 Copper(II) chloride Inorganic materials 0.000 claims description 4
- 241001481789 Rupicapra Species 0.000 claims description 4
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 4
- 238000012512 characterization method Methods 0.000 claims description 4
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims description 4
- 125000004122 cyclic group Chemical group 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims description 4
- 235000019441 ethanol Nutrition 0.000 claims description 4
- 239000010985 leather Substances 0.000 claims description 4
- 230000003647 oxidation Effects 0.000 claims description 4
- 238000007254 oxidation reaction Methods 0.000 claims description 4
- 238000005498 polishing Methods 0.000 claims description 4
- 229920000193 polymethacrylate Polymers 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 230000009467 reduction Effects 0.000 claims description 4
- 238000004506 ultrasonic cleaning Methods 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 238000003786 synthesis reaction Methods 0.000 claims description 3
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 claims description 2
- 101150096185 PAAS gene Proteins 0.000 claims 1
- 229920002845 Poly(methacrylic acid) Polymers 0.000 claims 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 abstract description 10
- 229910052802 copper Inorganic materials 0.000 abstract description 10
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 abstract description 10
- 238000010408 sweeping Methods 0.000 abstract description 5
- 239000011159 matrix material Substances 0.000 abstract description 2
- 239000002086 nanomaterial Substances 0.000 abstract description 2
- 230000027756 respiratory electron transport chain Effects 0.000 abstract description 2
- 238000000502 dialysis Methods 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 10
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 8
- 239000008055 phosphate buffer solution Substances 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- 238000002484 cyclic voltammetry Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 4
- 229960005070 ascorbic acid Drugs 0.000 description 4
- 235000010323 ascorbic acid Nutrition 0.000 description 4
- 239000011668 ascorbic acid Substances 0.000 description 4
- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 description 3
- 230000005518 electrochemistry Effects 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 229960003638 dopamine Drugs 0.000 description 2
- 238000002189 fluorescence spectrum Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000011896 sensitive detection Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000004832 voltammetry Methods 0.000 description 2
- 208000010444 Acidosis Diseases 0.000 description 1
- 238000004847 absorption spectroscopy Methods 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000000627 alternating current impedance spectroscopy Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000002969 morbid Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- -1 potassium ferricyanide Chemical compound 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 150000007968 uric acids Chemical class 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
Abstract
The present invention is disclosed based on the uric acid electrochemical sensor containing active copper carbon dots and its application.The present invention will be enriched in the high molecular polymer of carboxyl, be added in reaction vessel containing cupric salt, be placed in hydrothermal reaction kettle, and 2~18h, centrifugation are reacted at 200~240 DEG C, and dialysis purification is obtained containing active copper carbon dots solution；Using three-electrode system, using glass-carbon electrode as working electrode, platinum electrode is to electrode, saturated calomel electrode is reference electrode, above-mentioned pretreated glass-carbon electrode is placed in the above-mentioned solution containing active copper carbon dots, using electrodeposition process 50~500mV/s of potential range of 0~2V scan round under sweeping speed.Compared with prior art, the present invention utilizes the specific surface area for not only having carbon dots big containing this nano material of active copper carbon dots and high electron transport rate, and also active copper is to the facilitation of the electron transfer rate of matrix on electrode interface and to the very strong selectivity of uric acid.
Description
Technical field
The invention belongs to electrochemica biological sensor technical fields, and in particular to based on the carbon dots electrochemistry containing active copper
The selective Sensitive Detection to uric acid is realized in sensor and its application.
Background technique
Uric acid is the metabolite of human body purine, will lead to urarthritis, high urine if too high levels in human body
The diseases such as acidaemia and Lesh Nyan syndrome.Recently as the raising of people's quality of life, our people takes the photograph from food
The purine content taken improves, and more and more people are in the excessively high sub-health state of uric acid even morbid state.Therefore, in human body
The accurate detection of uric acid concentration is particularly important in clinical diagnosis field.
The measuring method of uric acid concentration mainly has chromatography, spectrophotometry, fluorescence method, chemoluminescence method, electrification at present
Method.Wherein electrochemical method has many advantages, such as the good, high sensitivity of selectivity, easy to operate and low in cost and be widely used in
The measurement of uric acid.However the method for prior art electrochemical sensor detection uric acid mainly encounters two problems: on the one hand, tradition
The current potential of its oxidizing potential and ascorbic acid is close on electrode, is easy the interference by ascorbic acid；On the other hand, uric acid detects
Sensitivity limited be unable to meet demand.
Summary of the invention
It is an object of the present invention to solve in the presence of the prior art, provide a kind of based on containing living
Property copper carbon dots (Cu-CDs) uric acid electrochemica biological sensor, by the selectivity of active copper and carbon dots improving above sensitivity
Contribution combine.It is final to realize to the highly sensitive detection of the selectivity of uric acid, have good, the high sensitivity stability of selectivity and
The advantages that preparation process is simple, at low cost.
It is provided by the invention based on the carbon dots electrochemical sensor containing active copper, using the preparation of following methods step
At:
1) active copper carbon dots are contained using hydro-thermal method one-step synthesis:
Will be enriched in the high molecular polymer of carboxyl, be added in reaction vessel containing cupric salt, then be added 50ml go from
Sub- water dilution, obtains mixed reaction solution after mixing；Mixed reaction solution is poured into hydrothermal reaction kettle again, at 200~240 DEG C
2~18h is reacted, mixed liquor is obtained；Gained mixed liquor 13000rpm is centrifuged the bulky grain residue in 30 minutes removal solution；With
The bag filter that molecular weight is 500~3000D dialyses 10~30h to achieve the purpose that purifying, is finally kept in dark place, obtains at 4 DEG C
To the solution containing active copper carbon dots；
Described is Cu (NO containing cupric salt3)2、CuCl2、CuSO4Deng；
The high molecular polymer is sodium polymethacrylate (PMAA), Sodium Polyacrylate (PAAS) etc.；
The high molecular polymer and the mass ratio containing cupric salt is 1:0.02~0.1；
The concentration of bivalent cupric ion is 1~10.2mgmL in the mixed reaction solution-1。
2) preparation of electrochemica biological sensor:
1. the pretreatment of glass-carbon electrode: cooperating chamois leather polishing with the alumina powder that partial size is 1.0,0.3,0.05 μm respectively
Electrode, in 0.65mM (concentration range is in 0.1mmol~1mmol) K3[Fe(CN)6]/K4[Fe(CN)6] carry out in solution
Cyclic voltammetric characterization after the peak difference △ Ep < 75mV of oxidation peak and reduction peak, then successively uses acetone, ethyl alcohol and deionized water
Ultrasonic cleaning, it is finally stand-by with being dried with nitrogen.
2. the preparation of electrochemica biological sensor: using three-electrode system, using glass-carbon electrode as working electrode, platinum electrode
For to electrode, saturated calomel electrode is reference electrode, by containing for above-mentioned pretreated glass-carbon electrode merging step (1) preparation
In the solution of active copper carbon dots, using electrodeposition process 50~500mV/s of potential range of 0~2V scan round 2 under sweeping speed
~20 circles, to be made based on the electrochemica biological sensor containing active copper carbon dots.
It is a further object to provide above-mentioned based on the electrochemica biological sensor containing active copper carbon dots, is examining
The application on uric acid is surveyed, specifically includes the following steps:
1) using phosphate buffer solution as bottom liquid, the uric acid prepare liquid of different pH is configured, by electrochemica biological sensor
Three electrodes connection electrochemical workstation is inserted into the solution, is tested, is obtained with electrochemical response using cyclic voltammetry
Cyclic voltammetry curve, compare uric acid peak current size and pH relationship, find out optimal pH.
2) using phosphate buffer solution as bottom liquid, uric acid prepare liquid is configured, three electrodes of electrochemica biological sensor are connected
It connects electrochemical workstation to be inserted into the solution, changes scanning speed and tested using cyclic voltammetry, obtained with electrochemistry
The cyclic voltammetry curve of response compares the relationship of uric acid peak current size and scanning speed, finds out and most preferably sweep fast value.
3) using phosphate buffer solution as bottom liquid, a series of different uric acid prepare liquids under optimal pHs are configured, by electrochemistry
In the three electrodes connection electrochemical workstation insertion prepare liquid of biosensor, voltammetry is used under optimum scanning speed
It is tested, obtains the voltage-to-current working curve with electrochemical response characteristic peak, therefrom obtain concentration-peak current relationship
Working curve.
Compared with prior art, the present invention utilizes the ratio for not only having carbon dots big containing this nano material of active copper carbon dots
Surface area and high electron transport rate, facilitation of the also active copper to the electron transfer rate of matrix on electrode interface
And the very strong selectivity to uric acid.Biosensor is prepared based on this principle, is realized to uric acid sensitivity, specificity
With the measurement of stability.
Detailed description of the invention
Fig. 1 is the experimental principle schematic diagram that uric acid is detected based on Cu-CDs electrochemical sensor；
(illustration is Cu-CDs under ultraviolet light irradiation to the fluorescence spectrum and ultraviolet-visible absorption spectroscopy that Fig. 2 is Cu-CDs
Picture)；
Fig. 3 is the ac impedance spectroscopy of different modifying electrode；
Fig. 4 is cyclic voltammogram of the Different electrodes in uric acid solution；
Fig. 5 is influence diagram of the pH to this experiment；
Fig. 6 is influence diagram of the scanning speed to this experiment；
Fig. 7 is influence diagram of the uric acid to this experiment of various concentration；
Fig. 8 is the uric acid of various concentration and the linear relationship chart of peak current；
Fig. 9 is this method to dopamine, the selective comparison diagram of ascorbic acid and uric acid.
Specific embodiment
The present invention is further analyzed with attached drawing combined with specific embodiments below.
Embodiment 1
Preparation method based on the carbon dots electrochemica biological sensor containing active copper, comprising the following steps:
A, the preparation containing active copper carbon dots: contain the carbon dots of active copper using hydro-thermal method one-step synthesis: weighing first
2.5g PMAA and 50mg Cu (NO3)2In beaker, the dilution of 50ml deionized water, thermal response to be mixed of uniformly falling back is added
In kettle, 240 DEG C of reaction 9h.Products therefrom 13000rpm is centrifuged the bulky grain residue in 30 minutes removal solution.Use molecular weight
12h is dialysed to achieve the purpose that purifying for the bag filter of 1000D, is finally kept in dark place at 4 DEG C.Using fluorescence spectrum and ultraviolet
Visible absorption spectra is characterized to what is obtained containing active copper carbon dots, as a result as shown in Figure 2.
B, the pretreatment of glass-carbon electrode: cooperate chamois leather polishing with the alumina powder that partial size is 1.0,0.3,0.05 μm respectively
Electrode, in 0.65mM K3[Fe(CN)6]/K4[Fe(CN)6] cyclic voltammetric characterization is carried out in solution, when oxidation peak and reduction peak
It is finally stand-by with being dried with nitrogen after peak difference △ Ep < 75mV, then successively with acetone, ethyl alcohol and deionized water ultrasonic cleaning.
C, three-electrode system, using glass-carbon electrode as working electrode, platinum electrode the preparation of electrochemica biological sensor: are used
For to electrode, saturated calomel electrode is reference electrode, contain work for prepared by above-mentioned pretreated glass-carbon electrode merging step 1
Property copper carbon dots solution in, the scan round 10 under speed of sweeping using electrodeposition process in the potential range 100mV/s of 0~2V is enclosed, from
And it is made based on the electrochemica biological sensor containing active copper carbon dots.
Under experiment condition same as Example 1, electrochemica biological sensor is placed in potassium ferricyanide solution, 0.01
Scanning in the range of scanned frequencies of~100KHz, as a result as shown in Figure 3.
Under experiment condition same as Example 1, electrochemica biological sensor is placed in 0.1mol/L PBS solution (pH
7.0) it in, being carried out in -0.4~0.6 potential range, sweeping for 100mV/s carries out under speed, corresponding cyclic voltammogram is recorded,
As a result as shown in Figure 4.
Embodiment 2
By 50mg Cu (NO3)2Become 1500mg Cu (NO3)2, other conditions are made with embodiment 1 to be based on containing active copper
The electrochemica biological sensor of carbon dots.
Embodiment 3
By 50mg Cu (NO3)2Become 36.4mg CuCl2, other conditions are made with embodiment 1 to be based on containing active copper carbon
The electrochemica biological sensor of point.
Embodiment 4
By 50mg Cu (NO3)2Become 1092mg CuCl2, other conditions are made with embodiment 1 to be based on containing active copper carbon
The electrochemica biological sensor of point.
Embodiment 5
By 50mg Cu (NO3)2Become 42.6mg CuSO4, other conditions are made with embodiment 1 to be based on containing active copper carbon
The electrochemica biological sensor of point.
Embodiment 6
By 50mg Cu (NO3)2Become 1278mg CuSO4, other conditions are made with embodiment 1 to be based on containing active copper carbon
The electrochemica biological sensor of point.
Embodiment 7
Using the concentration of the sensor detection uric acid of above-mentioned preparation, as shown in Figure 1:
A, it prepares uric acid solution: the uric acid of purchase is dissolved with phosphate buffer solution, being configured to concentration is 100 μm of ol/L
Solution, 4 DEG C save it is stand-by；The preparation of 0.1mol/L phosphate buffer solution (PBS): NaH is used2PO4, Na2HPO4Match with KCl
System, and with 0.10mol/L H3PO4Or NaOH adjusts pH value, 4 DEG C save for use.
B, the pretreatment of glass-carbon electrode: cooperate chamois leather polishing with the alumina powder that partial size is 1.0,0.3,0.05 μm respectively
Electrode, in 0.65mM K3[Fe(CN)6]/K4[Fe(CN)6] cyclic voltammetric characterization is carried out in solution, when oxidation peak and reduction peak
It is finally stand-by with being dried with nitrogen after peak difference △ Ep < 75mV, then successively with acetone, ethyl alcohol and deionized water ultrasonic cleaning.
C, three-electrode system, using glass-carbon electrode as working electrode, platinum electrode the preparation of electrochemica biological sensor: are used
For to electrode, saturated calomel electrode is reference electrode, by above-mentioned pretreated glass-carbon electrode merging 1 step A of embodiment preparation
The carbon dots solution containing active copper in, using electrodeposition process the potential range 100mV/s of 0~2V scan round under sweeping speed
10 circles, so that the electrochemica biological sensor based on the carbon dots containing active copper be made.
D, using phosphate buffer solution (pH=7.0) as bottom liquid, a series of uric acid prepare liquid of various concentrations is configured, it will be upper
It states in electrochemica biological sensor connection electrochemical workstation insertion prepare liquid, letting nitrogen in and deoxidizing, under the scanning speed of 100mV/s
(parameter are as follows: sweep speed 50mV/s, amplification 0.05V, pulse width 0.05s scan model is tested using voltammetry
Enclose -0.12~0.25V).The peak current and concentration measured establishes linear relationship (Fig. 7 and Fig. 8), then tests under the same terms,
The peak current for measuring unknown concentration uric acid, the concentration of uric acid is obtained according to the concentration of foundation-peak current relationship working curve.
Embodiment 8
Change the pH value of buffer solution, for other steps with embodiment 2, influence of the pH value to this experiment is as shown in Figure 5；
Change scanning speed, for other steps with embodiment 2, influence of the scanning speed to this experiment is as shown in Figure 6；
Embodiment 9
Will test object uric acid and be changed to ascorbic acid and dopamine respectively, same experimental example 2 under the same conditions, as a result such as
Shown in Fig. 9.
Above-described embodiment is not for limitation of the invention, and the present invention is not limited only to above-described embodiment, as long as meeting
The present invention claims all belong to the scope of protection of the present invention.
Claims (4)
1. being prepared based on the uric acid electrochemical sensor containing active copper carbon dots using following methods:
Step (1) contains active copper carbon dots using hydro-thermal method one-step synthesis:
It will be enriched in the high molecular polymer of carboxyl, be added in reaction vessel containing cupric salt, deionized water dilution is then added,
Mixed reaction solution is obtained after mixing；Mixed reaction solution is poured into hydrothermal reaction kettle again, at 200~240 DEG C react 2~
18h obtains mixed liquor, centrifugation removal bulky grain residue；10~30h is dialysed with the bag filter that molecular weight is 500~3000D to reach
It to the purpose of purifying, is kept in dark place, obtains the solution containing active copper carbon dots；
The preparation of step (2), electrochemical sensor:
(2.1) pretreatment of glass-carbon electrode: being 0.1mmol in concentration respectively with alumina powder cooperation chamois leather polishing electrode
The K of~1mmol3[Fe(CN)6]/K4[Fe(CN)6] cyclic voltammetric characterization is carried out in solution, when the peak value of oxidation peak and reduction peak
It is finally stand-by with being dried with nitrogen after poor △ Ep < 75mV, then successively with acetone, ethyl alcohol and deionized water ultrasonic cleaning；
(2.2) preparation of electrochemical sensor: three-electrode system is used, using glass-carbon electrode as working electrode, platinum electrode is
To electrode, saturated calomel electrode is reference electrode, and above-mentioned pretreated glass-carbon electrode merging step (1) preparation is contained work
In the solution of property copper carbon dots, using electrodeposition process 0~2V 50~500mV/s of potential range sweep scan round 2 under speed~
20 circles, to be made based on the electrochemical sensor containing active copper carbon dots；
The carboxylic high molecular polymer of richness described in step (1) and the mass ratio containing cupric salt are 1:0.02~0.1；
The concentration of bivalent cupric ion is 1~10.2mgmL in mixed reaction solution described in step (1)-1。
2. as described in claim 1 based on the uric acid electrochemical sensor containing active copper carbon dots, it is characterised in that step (1)
Described is Cu (NO containing cupric salt3)2、CuCl2Or CuSO4。
3. as described in claim 1 based on the uric acid electrochemical sensor containing active copper carbon dots, it is characterised in that step (1)
The carboxylic high molecular polymer of richness is sodium polymethacrylate PMAA or Sodium Polyacrylate PAAS.
4. as described in claim 1 based on the uric acid electrochemical sensor containing active copper carbon dots, answering on detection uric acid
With.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710279857.5A CN107102052B (en) | 2017-04-26 | 2017-04-26 | Based on the uric acid electrochemical sensor containing active copper carbon dots and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710279857.5A CN107102052B (en) | 2017-04-26 | 2017-04-26 | Based on the uric acid electrochemical sensor containing active copper carbon dots and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107102052A CN107102052A (en) | 2017-08-29 |
| CN107102052B true CN107102052B (en) | 2019-08-09 |
Family
ID=59657006
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710279857.5A Active CN107102052B (en) | 2017-04-26 | 2017-04-26 | Based on the uric acid electrochemical sensor containing active copper carbon dots and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107102052B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109705858B (en) * | 2019-01-04 | 2020-05-01 | 河南大学 | Preparation method and application of copper-containing carbon dots |
| CN110339357B (en) * | 2019-02-28 | 2021-04-13 | 安徽大学 | Copper ion doped carbon dot, preparation and application thereof as photodynamic therapy photosensitizer |
| CN112458518B (en) * | 2020-11-20 | 2023-07-28 | 昆明理工大学 | Preparation method of high-conductivity copper-based composite material |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105628764B (en) * | 2016-02-25 | 2018-07-24 | 衡阳师范学院 | A kind of electrochemical sensor of detection uric acid and its preparation and application |
-
2017
- 2017-04-26 CN CN201710279857.5A patent/CN107102052B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN107102052A (en) | 2017-08-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107153089B (en) | A kind of preparation method of dendroid nano-complex Doxorubicin electrochemical sensor | |
| CN107037102B (en) | Nano composite material and preparation method and application thereof | |
| CN107102052B (en) | Based on the uric acid electrochemical sensor containing active copper carbon dots and its application | |
| CN106501332B (en) | Zn-MOFs/ glass-carbon electrode and preparation method thereof and application | |
| CN104198551B (en) | Make Platinum Nanoparticles and the glass-carbon electrode of multi-walled carbon nano-tubes modification and the method using this electrode detection estradiol | |
| CN101793862B (en) | L-cysteine/chitosan-modified electrochemical sensor and use thereof | |
| CN109490385A (en) | Biosensor and preparation method thereof based on Au-ZIF-8/OMC mesoporous carbon | |
| CN108572209A (en) | Electrochemica biological sensor electrode and preparation method thereof, electrochemica biological sensor and its application | |
| CN103175884A (en) | High-sensitivity glucose biosensor and preparation method thereof | |
| CN109254041A (en) | The electrochemical detection method of capsaicine in a kind of capsicum | |
| CN104833718A (en) | Preparation method and application of pH release-type immunosensor | |
| CN112432981A (en) | Single-cell electrochemical sensor based on functionalized nanoprobe and application thereof | |
| CN106645354A (en) | Preparation method and application of heavy metal ion electrochemical sensor | |
| CN110441364A (en) | A kind of ultra-thin MXene nano material and its preparation method and application | |
| CN110186966A (en) | A kind of preparation method and application for the composite material modified electrode detecting lactic acid concn | |
| CN106442667A (en) | Method for detecting blood glucose by virtue of persimmon tannin@graphene@Pt-Pd non-enzymatic sensor | |
| CN113588752B (en) | Preparation method and application of electrochemiluminescence aptamer sensor | |
| CN107727720A (en) | HKUST‑1（Cu‑MOFs）Application in glucose sensor electrode is prepared | |
| CN104237361B (en) | Detect Cd based on Cys/graphene modified electrode simultaneously2+、Pb2+Electrochemical method | |
| CN111239212B (en) | Ciprofloxacin detection method | |
| CN110441373A (en) | A kind of preparation method of oxidation of ethanol enzyme modification composite paste electrode sensor | |
| CN104198706A (en) | Method for preparing electrochemical immunosensor for detecting tumor markers | |
| CN110988069B (en) | Working electrode and preparation method thereof, sensor based on working electrode and detection method | |
| CN102621216A (en) | Method for detecting trace terramycin with double-amplification-effect molecular imprinting electrochemical sensor | |
| CN112649482A (en) | Enzyme-free electrochemical urea sensor based on Ni-MOFs composite material and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201113 Address after: 310012 room 2603, building 8, No. 2, xiyuanba Road, Sandun Town, Xihu District, Hangzhou City, Zhejiang Province Patentee after: HANGZHOU ZHUILIE TECHNOLOGY Co.,Ltd. Address before: Hangzhou City, Zhejiang province 310018 Xiasha Higher Education Park No. 2 street Patentee before: HANGZHOU DIANZI University |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20201217 Address after: 314500 Zhejiang, Jiaxing, Tongxiang, Wu Tung Street revitalization East Road Fengrun international business center, room 635, -48 Patentee after: Jiaxing bincong Trading Co.,Ltd. Address before: Room 2603, building 8, No.2, Xiyuan 8th Road, Sandun Town, Xihu District, Hangzhou City, Zhejiang Province Patentee before: HANGZHOU ZHUILIE TECHNOLOGY Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221209 Address after: Room 407-10, floor 4, building 2, Haichuang science and technology center, Cangqian street, Yuhang District, Hangzhou City, Zhejiang Province, 311100 Patentee after: Zhejiang Zhiduo Network Technology Co.,Ltd. Address before: 314500 Zhejiang, Jiaxing, Tongxiang, Wu Tung Street revitalization East Road Fengrun international business center, room 635, -48 Patentee before: Jiaxing bincong Trading Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230529 Address after: No. 8 Shangde Road, Xinbei District, Changzhou City, Jiangsu Province, 213132 Patentee after: AIKESI ELECTRONIC TECHNOLOGY (CHANGZHOU) Co.,Ltd. Address before: Room 407-10, floor 4, building 2, Haichuang science and technology center, Cangqian street, Yuhang District, Hangzhou City, Zhejiang Province, 311100 Patentee before: Zhejiang Zhiduo Network Technology Co.,Ltd. |
|
| TR01 | Transfer of patent right |