CN108254235A - Array sample injector - Google Patents

Array sample injector Download PDF

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Publication number
CN108254235A
CN108254235A CN201810023012.4A CN201810023012A CN108254235A CN 108254235 A CN108254235 A CN 108254235A CN 201810023012 A CN201810023012 A CN 201810023012A CN 108254235 A CN108254235 A CN 108254235A
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CN
China
Prior art keywords
liquid storage
sample
several
reagent
storage pipe
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Granted
Application number
CN201810023012.4A
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Chinese (zh)
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CN108254235B (en
Inventor
孟春
钟乐
王航
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Fuzhou University
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Fuzhou University
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Priority to CN201810023012.4A priority Critical patent/CN108254235B/en
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Publication of CN108254235B publication Critical patent/CN108254235B/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/021Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses array sample injectors,It includes several sample needles,Several liquid storage modules,Several feed drive devices and air source,Several liquid storage modules are corresponded and are connect with several sample needles,One end of several feed drive devices is connect with air source,The other end of several feed drive devices and several liquid storage modules, which correspond and connect and be used to liquid storage mould reagent in the block pushing to sample needle, carries out output sample-adding,It is realized different reagents and is loaded using different reagent needles,Eliminate the process of reagent needle cleaning,Reduce sample-adding required time and simplify motion structure,So as to so as to be reduced the time required to sample-adding every time,It ensure that the gap of the different slice contact Reagent Times during a variety of different first antibody reagent dropwises greatly reduces,It ensure that the consistency and repeatability of coloration result,The reagent and the contact area of air stored in instrument operational process is minimum,Ensure the safety of reagent and pure property.

Description

Array sample injector
Technical field
The present invention relates to biological medicine technologies to test instrument field, especially array sample injector.
Background technology
Expression is an important colouring method in pathology technique, has been obtained in pathology work Extensive use is that Pathology Doctors ' makes disease one of important supplementary means of diagnosis.For Pathology Lab, often Carry out a large amount of immunohistochemical staining work.
In the experiment of current immunohistochemical study and detection, conventional colouring method generally comprises following steps:It will Histotomy is by roasting piece, dewaxing treatment;Then it as the first antibody reagent corresponding to reagent needle extracts the histotomy, is added dropwise Onto histotomy, it is laggard cross be incubated, secondary antibody reagent dropwise, develop the color, redye after, observation dyeing under the microscope is tied Fruit;The action time length of wherein various reagents can impact coloration result.
In the above process, first antibody reagent dropwise, secondary antibody be added dropwise, develop the color, redying and etc. be directed to experiment The dropwise addition of reagent.The existing single reagent needle of immunohistochemistry self-reacting device generally use realizes the above-mentioned course of work.Each step Sample-adding process reagent needle needs to be repeated cleaning, imbibition, dropwise addition, cleaning.It has a defect that (1) reagent needle is anti- Liquid and cleaning process is taken to take considerable time again, especially when miscellaneous first antibody reagent is added dropwise, can cause to be sliced It in the case of different dropwise addition sequences, is sliced and differs greatly with the time of contact of reagent, influence the consistency of result with repeating Property;(2) it is halfway in the case that cleaning since same reagent needle extracts reagent in different reagent containers, easily cause reagent Cross contamination;(3) draw the time that the motion process of reagent and cleaning reagent needle expends cause the slice handled at the same time compared with It is more more than completing monolithic increase to complete the intact immune histochemical staining time for instrument when more;(4) due to during automatic operating Reagent container need to keep uncapped state, and reagent has larger area ingress of air, easily polluted by the floating material in air.
Invention content
For problem of the prior art, simplify the purpose of the present invention is to provide a kind of sample-adding process, be loaded it is efficient And the array sample injector that sample-adding process can be effectively prevent to pollute experiment reagent, possesses what is arranged in array Sample needle, the liquid storage module that each sample needle can store required reagent with an independence are connect, and each liquid storage module is correspondingly connected with Have feed drive device, overcome by this way existing immunohistochemistry self-reacting device sample injector there are the defects of.
In order to realize above-mentioned technical purpose, the technical solution adopted by the present invention is:
A kind of array sample injector, it is gentle including several sample needles, several liquid storage modules, several feed drive devices Source, several described sample needles are arranged in array, several liquid storage modules are corresponded with several sample needles and liquid storage mould The outlet end of block is connect with sample needle, and one end of several feed drive devices is connect with air source, several conveyings are driven The other end of dynamic device, which corresponds with several liquid storage modules and connect and be used to push to liquid storage mould reagent in the block, to be added Sample needle carries out output sample-adding.
Further, sample needle installing plate is further included, several described sample needles place arrangement in array and are fixed on On sample needle installing plate.
Further, falsework, translation mechanism and elevating mechanism are further included, the falsework is in frame structure, The elevating mechanism is connected to the side of falsework, and the translation mechanism connect with elevating mechanism, driven by elevating mechanism Translation mechanism moves up and down in falsework, and the sample needle installing plate is fixedly connected with translation mechanism, by translation mechanism Driving sample needle installing plate drives sample needle to do translational motion along horizontal plane where translation mechanism.
Preferably, the liquid storage module includes liquid storage pipe, air rammer, liquid storage pipe installing plate, liquid storage pipe lid, the storage One end of liquid pipe is fixedly connected with liquid storage pipe installing plate and one end of liquid storage pipe passes through liquid storage pipe installing plate by connector and leads to It crosses liquid line to connect connector with sample needle pipeline, the liquid storage pipe lid is fixed on the other end of liquid storage pipe, liquid storage mould Block is connect with air source pipeline and pipeline is set in the other end of liquid storage pipe and is fixedly connected with liquid storage pipe lid, the pneumatic work Plug is slidably arranged in liquid storage pipe, and when the gas of air source is sent into liquid storage pipe, air rammer pushes the reagent in liquid storage pipe defeated Go out.
Preferably, the pipeline side being set in the liquid storage pipe other end is equipped with the O with liquid storage pipe madial wall palette Type sealing ring.
Preferably, the threeway for source gas to be controlled to transmit is additionally provided on the connecting line of the liquid storage module and air source Valve.
More preferably, the triple valve is Electrocontrolled tee valve.
Preferably, solenoid valve cylinder manifold is equipped between the triple valve and air source, the triple valve is each attached to electricity On magnet valve cylinder manifold and pass through solenoid valve cylinder manifold and connect with air source pipeline.
Preferably, the triple valve is wherein connect with solenoid valve cylinder manifold all the way, the another way and liquid storage pipe of triple valve The other end pipeline connection, triple valve residue communicated all the way with air.
Preferably, the air source that the triple valve is correspondingly connected with is individual gas sources or is that several feed drive devices share Air source.
Using above-mentioned technical solution, beneficial effects of the present invention are:
(1)Different reagents are realized by array sample injector of the present invention to be loaded using different reagent needles, eliminate examination The process of agent needle cleaning, the time required to reducing sample-adding;
(2)Pipeline transport reagent has been used by array sample injector of the present invention, has eliminated the mistake using reagent needle suction reagent Journey, loading time simultaneously simplify motion structure;
(3)It can make to reduce the time required to being loaded every time by array sample injector of the present invention, ensure that a variety of different first Antibody reagent during being added dropwise the gaps of different slice contact Reagent Times greatly reduce, ensure that the consistency of coloration result with Repeatability;
(4)Since array sample injector of the present invention is using Pipeline transport reagent, the reagent stored in instrument operational process with The contact area of air is minimum so that its can effectively prevent from contacting with air caused by reagent contamination, ensure that reagent Safety and pure property.
Description of the drawings
The present invention is further elaborated with reference to the accompanying drawings and detailed description:
Fig. 1 is the brief configuration schematic diagram of array sample injector of the present invention;
Fig. 2 is the structure simplified diagram at the wherein side visual angle of array sample injector of the present invention;
Fig. 3 is the structure simplified diagram at the wherein opposite side visual angle of array sample injector of the present invention;
Cooperation principle simplified diagrams of the Fig. 4 for the sample needle of array sample injector of the present invention, liquid storage module and air source;
Fig. 5 is that the structure when sample needle of array sample injector of the present invention is arranged in array on sample needle installing plate is briefly shown It is intended to.
Specific embodiment
As shown in Fig. 1 to one of 5, the present invention includes several sample needles 1, several liquid storage modules, several conveying drives Dynamic device and air source 8, several described sample needles 1 are arranged in array, several liquid storage modules and several sample needles 1 are one by one Corresponding and liquid storage module outlet end is connect with sample needle 1, and one end of several feed drive devices connects with air source 8 It connects, the other end of several feed drive devices and several liquid storage modules are corresponded and connect and be used for will be in liquid storage module Reagent push to sample needle 1 and carry out output sample-adding, wherein, can be done between sample needle 1, liquid storage module and feed drive device Sample needle 1 with liquid storage module by liquid line 13 can also be attached, feed drive filled by integral integrated structure It puts and is connect with air source 8 by pipeline, in the form of this forms split structure.
Further, sample needle installing plate 15 is further included, several described sample needles 1 place arrangement in array and fix On sample needle installing plate 15.
Further, falsework 10, translation mechanism and elevating mechanism are further included, the falsework 10 is in frame knot Structure, the elevating mechanism are connected to the side of falsework 10, and the translation mechanism is connect with elevating mechanism, by elevating mechanism Translation mechanism is driven to be moved up and down in falsework 10, the sample needle installing plate 15 is fixedly connected with translation mechanism, by Translation mechanism driving sample needle installing plate 15 drives sample needle 1 to do translational motion along horizontal plane where translation mechanism, wherein, translation Mechanism and elevating mechanism are driven movement by motor 9,14.
Additionally, it is preferred that, the liquid storage module includes liquid storage pipe 2, air rammer 3, liquid storage pipe installing plate 4, liquid storage pipe Lid 5, one end of the liquid storage pipe 2 is fixedly connected with liquid storage pipe installing plate 4 and 2 one end of liquid storage pipe pass through liquid storage by connector Pipe installing plate 4 simultaneously passes through liquid line 13 and connect connector with 1 pipeline of sample needle, and the liquid storage pipe lid 5 is fixed on liquid storage The other end of pipe 2, liquid storage module are set in the other end of liquid storage pipe 2 with the pipeline that air source 8 is connect and are fixed with liquid storage pipe lid 5 Connection, the air rammer 3 are slidably arranged in liquid storage pipe 2, when the gas of air source 8 is sent into liquid storage pipe 1, air rammer 2 push the reagent output in liquid storage pipe 1, it is preferred that the pipeline side being set in 2 other end of liquid storage pipe is equipped with and liquid storage pipe The O-ring seal 7 of 2 madial wall palettes, it is preferred that the liquid storage module is used for being additionally provided on the connecting line of air source 8 The triple valve 6 of 8 gas transport of air source is controlled, more preferably, the triple valve 6 is Electrocontrolled tee valve, it is preferred that described Solenoid valve cylinder manifold 11 is equipped between triple valve 6 and air source 8, the triple valve 6 is each attached on solenoid valve cylinder manifold 11 simultaneously Connect by solenoid valve cylinder manifold 11 with 8 pipeline of air source, it is preferred that the triple valve 6 wherein all the way with solenoid valve cylinder manifold 11 connections, the another way of triple valve 6 are connect with the other end pipeline of liquid storage pipe 2, and the residue of triple valve 6 communicates all the way with air, When triple valve 6 is powered, 5 interface of liquid storage pipe lid connects tracheae 12(The pipeline that liquid storage module is connect with air source is tracheae)With air source 8 connections, when triple valve 6 powers off, liquid storage pipe lid interface connects tracheae 12 and is communicated with air, in addition, in order to improve the choosing of air source 8 Selecting property, the air source 8 that the triple valve 6 is correspondingly connected with can be individual gas sources or be the gas that several feed drive devices share Source.
The operation principle of array sample injector of the present invention is as follows:
When needing sample-adding, the rotation of motor 9,14 is controlled to drive elevating mechanism and translation mechanism on falsework by controller (Two-dimensional stage motion)It is moved, so as to move sample needle installing plate 15, and passes through the fortune of sample needle installing plate 15 The dynamic sample needle 1 by corresponding to the fixed liquid storage module with the required sample-adding reagent of storage on sample needle installing plate 15, which is moved to, to be needed Above the histotomy tissue position for adding the reagent.
After moving in place, controller controls the triple valve 6 corresponding to the liquid storage module of sample-adding reagent needed for above-mentioned storage It is powered so that the liquid storage module is connected with air source 8, and air source 8 is under the control of the controller by tracheae 12 into the liquid storage module 2 top of liquid storage pipe is passed through gas push air rammer 3 and moves downward, and required reagent is transported to by fluid pipeline 13 corresponding Syringe needle 1 and be loaded onto on histotomy.
When multiple slices need to be loaded a variety of different reagents, wherein a piece of slice for needing to be loaded is in the manner described above After completing corresponding reagent pipetting volume, the sample needle installing plate 15 moves under control of the controller, by sample needle installing plate 15 It is in place that sample needle 1 needed for upper fixed and next slice corresponding to reagent liquid storage module is moved to next histotomy tissue institute It puts and completes sample-adding in the manner described above, repeat this process and complete to need the sample-adding work of all histotomies of different reagents.
Array sample injector described in this example eliminate the absorption of repeated washing reagent syringe needle reagent to complete these steps Required motion process, the time required to shortening primary sample-adding, while ensure that the consistency of coloration result with repeatability Effectively raise working efficiency.
The above is the embodiment of the present invention, for the ordinary skill in the art, religion according to the present invention Lead, without departing from the principles and spirit of the present invention all equivalent changes done according to scope of the present invention patent, repair Change, replace and modification, should all belong to the covering scope of the present invention.

Claims (10)

1. a kind of array sample injector, it is characterised in that:It includes that several sample needles, several liquid storage modules, several are defeated Driving device and air source are sent, several described sample needles are arranged in array, several liquid storage modules and several sample needles one The outlet end of one correspondence and liquid storage module is connect with sample needle, and one end of several feed drive devices connects with air source It connects, the other end of several feed drive devices and several liquid storage modules are corresponded and connect and be used for will be in liquid storage module Reagent push to sample needle and carry out output sample-adding.
2. a kind of array sample injector according to claim 1, it is characterised in that:It further includes sample needle installing plate, institute Several sample needles stated place arrangement in array and are fixed on sample needle installing plate.
3. a kind of array sample injector according to claim 2, it is characterised in that:It further includes falsework, translation mechanism And elevating mechanism, the falsework are in frame structure, the elevating mechanism is connected to the side of falsework, the translation Mechanism is connect with elevating mechanism, translation mechanism is driven to be moved up and down in falsework by elevating mechanism, sample needle peace Loading board is fixedly connected with translation mechanism, and sample needle is driven along level where translation mechanism by translation mechanism driving sample needle installing plate Do translational motion in face.
4. a kind of array sample injector according to claim 3, it is characterised in that:The liquid storage module includes liquid storage Pipe, air rammer, liquid storage pipe installing plate, liquid storage pipe lid, one end of the liquid storage pipe are fixedly connected and store up with liquid storage pipe installing plate One end of liquid pipe passes through liquid storage pipe installing plate by connector and is connect with sample needle, and the liquid storage pipe lid is fixed on liquid storage pipe The other end, liquid storage module is connect with air source pipeline and pipeline is set in the other end of liquid storage pipe and is fixed with liquid storage pipe lid and connected It connects, the air rammer is slidably arranged in liquid storage pipe, and when the gas of air source is sent into liquid storage pipe, air rammer pushes storage Reagent output in liquid pipe.
5. a kind of array sample injector according to claim 4, it is characterised in that:The pipe being set in the liquid storage pipe other end Road side is equipped with the O-ring seal with liquid storage pipe madial wall palette.
6. a kind of array sample injector according to claim 4, it is characterised in that:The connection of the liquid storage module and air source The triple valve for source gas to be controlled to transmit is additionally provided on pipeline.
7. a kind of array sample injector according to claim 6, it is characterised in that:The triple valve is automatically controlled threeway Valve.
8. a kind of array sample injector according to claim 7, it is characterised in that:Between the triple valve and air source Equipped with solenoid valve cylinder manifold, the triple valve is each attached on solenoid valve cylinder manifold and passes through solenoid valve cylinder manifold and gas source pipe Road connects.
9. a kind of array sample injector according to claim 8, it is characterised in that:The triple valve wherein all the way with electricity Magnet valve cylinder manifold connects, and the other end pipeline of the another way of triple valve and liquid storage pipe connects, triple valve it is remaining all the way with air It communicates.
10. a kind of array sample injector according to claim 9, it is characterised in that:The gas that the triple valve is correspondingly connected with Source is individual gas sources or is the air source that several feed drive devices share.
CN201810023012.4A 2018-01-10 2018-01-10 Array type sample injector Expired - Fee Related CN108254235B (en)

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Application Number Priority Date Filing Date Title
CN201810023012.4A CN108254235B (en) 2018-01-10 2018-01-10 Array type sample injector

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CN108254235A true CN108254235A (en) 2018-07-06
CN108254235B CN108254235B (en) 2020-03-17

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114324851A (en) * 2021-10-09 2022-04-12 北京海格莱生物科技有限公司 Multi-channel slide specimen cleaning device, immunohistochemical specimen processor and method
WO2024044928A1 (en) * 2022-08-30 2024-03-07 深圳华大智造科技股份有限公司 Reagent storage device and reagent operation system

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1882827A (en) * 2003-11-18 2006-12-20 C2诊断公司 Syringe block for a liquid analyser, especially for blood tests
CN101596469A (en) * 2009-07-03 2009-12-09 烟台艾德康生物科技有限公司 Multi-channel loading device
CN103252264A (en) * 2013-05-03 2013-08-21 苏州杰诺曼博生物科技有限公司 Multichannel liquid sample injector
CN204255960U (en) * 2014-12-09 2015-04-08 深圳市爱康生物科技有限公司 Miniature sample injector
CN205679535U (en) * 2016-06-23 2016-11-09 贺州学院 A kind of sample needle for chemical illumination immunity analysis instrument
CN106423324A (en) * 2016-09-28 2017-02-22 长春迪瑞医疗科技股份有限公司 Fluid transfer device and fluid transfer method
CN206587779U (en) * 2016-11-28 2017-10-27 河南理工大学 A kind of manual continuous sample-adding device

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1882827A (en) * 2003-11-18 2006-12-20 C2诊断公司 Syringe block for a liquid analyser, especially for blood tests
CN101596469A (en) * 2009-07-03 2009-12-09 烟台艾德康生物科技有限公司 Multi-channel loading device
CN103252264A (en) * 2013-05-03 2013-08-21 苏州杰诺曼博生物科技有限公司 Multichannel liquid sample injector
CN204255960U (en) * 2014-12-09 2015-04-08 深圳市爱康生物科技有限公司 Miniature sample injector
CN205679535U (en) * 2016-06-23 2016-11-09 贺州学院 A kind of sample needle for chemical illumination immunity analysis instrument
CN106423324A (en) * 2016-09-28 2017-02-22 长春迪瑞医疗科技股份有限公司 Fluid transfer device and fluid transfer method
CN206587779U (en) * 2016-11-28 2017-10-27 河南理工大学 A kind of manual continuous sample-adding device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114324851A (en) * 2021-10-09 2022-04-12 北京海格莱生物科技有限公司 Multi-channel slide specimen cleaning device, immunohistochemical specimen processor and method
WO2024044928A1 (en) * 2022-08-30 2024-03-07 深圳华大智造科技股份有限公司 Reagent storage device and reagent operation system

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Granted publication date: 20200317