CN108251107B - Preparation of cobalt ion doped carbon quantum dot, obtained carbon quantum dot and application - Google Patents
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Abstract
The invention discloses preparation of cobalt ion doped carbon quantum dots and application of the obtained carbon quantum dots, wherein a carbon source, a nitrogen source and a cobalt source are dispersed in an organic solvent to carry out solvothermal reaction to obtain the cobalt ion doped carbon quantum dots; the preparation method is simple, does not need to introduce complex functional groups to carry out organic reaction, is only simple mixing heating reaction, and is suitable for industrial application; in addition, the obtained cobalt ion doped carbon quantum dot has very high fluorescence quantum yield which can reach 93 percent at most; meanwhile, the cobalt ion doped carbon quantum dot has good fluorescence performance in an organic solvent, but the fluorescence intensity is obviously quenched after a small amount of water is added, so that the cobalt ion doped carbon quantum dot can be used for detecting the water content in the organic solvent, and the detection limit can reach 0.01%.
Description
Technical Field
The invention relates to the field of nano fluorescent materials, in particular to carbon quantum dots, and particularly relates to preparation of cobalt ion doped carbon quantum dots, obtained carbon quantum and application.
Background
With the rapid development of nanotechnology, the novel nano fluorescent material is widely applied to the fields of cell marking, cell imaging, medical diagnosis, analysis and detection and the like due to the advantages of simple preparation, photobleaching resistance, high sensitivity, wide dynamic range and the like. The novel nano fluorescent material mainly comprises two main types: semiconductor quantum dots and carbon quantum dots, wherein the carbon quantum dots have fluorescence properties similar to those of the semiconductor quantum dots, and also have the advantages of low toxicity and good biocompatibility that the semiconductor quantum dots do not have, so the carbon quantum dots are widely concerned.
The carbon quantum dots refer to spheroidal nanoparticles with carbon as a main component and the size of less than 10 nm. At present, researchers use different carbon simple substances and organic matters as precursors to prepare fluorescent carbon quantum dots with different properties by adopting different preparation methods. Theoretically, carbon elements can be used as precursors of carbon quantum dots as long as the carbon elements are contained, and coal, apples, leaves, hair, flour, egg shell membranes, soybean milk, fruit juice and the like can be used for preparing the carbon quantum dots. The preparation method of the carbon quantum dot mainly comprises two methods of top-down method and bottom-up method. The "top-down" method refers to breaking up a bulk carbon material into carbon materials of 10nm or less by arc cutting, laser ablation, or the like; the 'bottom-up' principle is that different small-sized precursors are selected and slowly grow into carbon nano-particles with the size of less than 10nm through chemical reaction.
However, in the existing preparation method, the problem of low quantum yield of the carbon quantum dots is generally existed, which limits the application of the carbon quantum dots to a certain extent.
Disclosure of Invention
In order to overcome the problems, the inventors of the present invention have conducted intensive studies, and have obtained a cobalt ion-doped carbon quantum dot by reacting a carbon source, a nitrogen source, and a cobalt source in an organic solvent, wherein the cobalt ion-doped carbon quantum dot has advantages of uniform size and high fluorescence quantum yield (up to 93%), and the cobalt ion-doped carbon quantum dot has good fluorescence property in the organic solvent, but the fluorescence intensity is significantly quenched after a small amount of water is added, and thus, can be used for detecting the water content in the organic solvent, thereby completing the present invention.
One of the purposes of the present invention is to provide a preparation method of cobalt ion doped carbon quantum dots, which is embodied in the following aspects:
(1) a preparation method of cobalt ion doped carbon quantum dots comprises the following steps:
step 1, adding a carbon source, a nitrogen source and a cobalt source into an organic solvent to form a precursor solution;
and 3, carrying out post-treatment to obtain the cobalt ion doped carbon quantum dots.
(2) The production method according to the above (1), wherein, in the step 1, the carbon source is selected from organic substances containing carbon elements; preferably, the carbon source is selected from organic compounds containing-COOH and/or-COONa, such as organic compounds containing-COONa; more preferably, the carbon source is selected from one or more of sodium citrate, citric acid, oxalic acid and sodium oxalate, such as sodium citrate.
(3) The production method according to the above (1) or (2), wherein in the step 1, the nitrogen source is selected from nitrogen-containing organic substances, preferably from ethylenediamine and/or urea, for example, urea.
(4) The production method according to one of the above (1) to (3), wherein, in the step 1, the cobalt source is selected from cobalt ion salts, preferably one or more selected from cobalt chloride, cobalt sulfate, cobalt nitrate and cobalt acetate, such as cobalt chloride.
(5) The production process according to one of the above (1) to (4), wherein, in the step 1, the organic solvent is selected from one or more of benzene, toluene, xylene, ethanol, tetrahydrofuran and carbon tetrachloride, preferably from one or more of benzene, toluene and xylene, for example, toluene.
(6) The production method according to one of the above (1) to (5), wherein, in the precursor solution,
the molar concentration of the carbon source is 0.01-10mol/L, preferably 0.01-1mol/L, more preferably 0.05-0.5mol/L, such as 0.05-0.2 mol/L; and/or
The molar concentration of the nitrogen source is 0.01-10mol/L, preferably 0.02-2mol/L, more preferably 0.05-0.5mol/L, such as 0.1-0.3 mol/L; and/or
The cobalt source has a molar concentration of 0.05 to 10mol/L, preferably 0.05 to 0.5mol/L, more preferably 0.05 to 0.2mol/L, for example 0.1 mol/L.
(7) The production method according to one of the above (1) to (6), wherein,
in step 2, the reaction proceeds as follows: performing the reaction at 100-500 ℃ for 5-50 h, preferably at 150-300 ℃ for 8-24 h, more preferably at 180-220 ℃ for 10-14 h, for example, at 200 ℃ for 12 h; and/or
In step 3, the post-treatment comprises separation and drying, preferably, the separation is performed by using a cylindrical membrane separation filter or a centrifugal method, and more preferably, the drying is vacuum freeze drying or vacuum heat drying, such as vacuum freeze drying.
The second purpose of the present invention is to provide a cobalt ion doped carbon quantum dot, which is embodied in the following aspects:
(8) a cobalt ion-doped carbon quantum dot preferably prepared by the method of one of (1) to (7) above, wherein,
the average particle size of the cobalt ion doped carbon quantum dots is 2-10 nm, preferably 2-8 nm; and/or
The fluorescence quantum yield of the cobalt ion doped carbon quantum dots is more than 85%, preferably more than 90%; and/or
The cobalt ion doped carbon quantum dots emit orange-yellow fluorescence under the excitation of ultraviolet light.
One of the purposes of the present invention is to provide an application of the cobalt ion doped carbon quantum dot, which is embodied in the following aspects:
(9) the cobalt ion-doped carbon quantum dot obtained by the method in one of (1) to (7) or the application of the cobalt ion-doped carbon quantum dot in (8) is used as a fluorescent probe to analyze and detect the water content in an organic solvent.
(10) The use of (9) above, wherein the detection limit of the cobalt ion-doped carbon quantum dots on the water content in the organic solvent can reach 0.01%.
Drawings
Fig. 1 shows a transmission electron micrograph of cobalt ion-doped carbon quantum dots prepared in example 1;
fig. 2a to 2b respectively show a three-dimensional fluorescence spectrum and a corresponding color spectrum of the cobalt ion-doped carbon quantum dot prepared in example 1;
fig. 2c shows a fluorescent emission picture of cobalt ion-doped carbon quantum dots prepared in example 1 under natural light (left side) and ultraviolet light with a wavelength of 365nm (right side);
3 a-3 b respectively show a three-dimensional fluorescence spectrum and a corresponding color spectrum of the carbon quantum dots which are not doped with cobalt ions and prepared in comparative example 1;
fig. 3c shows a fluorescent emission picture of cobalt ion-undoped carbon quantum dots prepared in comparative example 1 under natural light (left side) and ultraviolet light of 365nm wavelength (right side);
fig. 4a to 4b respectively show a three-dimensional fluorescence spectrum and a corresponding color spectrum of the cobalt ion-doped carbon quantum dot prepared in comparative example 2;
fig. 4c shows a picture of the fluorescence emission of the cobalt ion-doped carbon quantum dots prepared in comparative example 2 under natural light (left side) and ultraviolet light of 365nm wavelength (right side);
fig. 5 shows an XPS spectrum of the cobalt ion-doped carbon quantum dot prepared in example 1;
FIGS. 6a to 6c are graphs showing calculation of fluorescence quantum yields of carbon quantum dots prepared in example 1, comparative example 1 and comparative example 2, respectively;
FIG. 7 is a graph showing the intensity of fluorescence emitted at 565nm (440nm excitation) in dioxane solution with 10% water content for cobalt ion doped carbon quantum dots prepared in example 1 as a function of time;
fig. 8 shows the fluorescence spectra of cobalt ion doped carbon quantum dots prepared in example 1 in dioxane with different water content;
FIG. 9 shows a two-dimensional plot of fluorescence quenching versus different water content;
fig. 10 shows a standard curve for measuring the water content in dioxane using cobalt ion doped carbon quantum dots prepared in example 1.
Detailed Description
The present invention will be described in further detail below with reference to examples and experimental examples. The features and advantages of the present invention will become more apparent from the description.
The invention provides a preparation method of cobalt ion doped carbon quantum dots, which comprises the following steps:
step 1, adding a carbon source, a nitrogen source and a cobalt source into an organic solvent to form a precursor solution.
According to a preferred embodiment of the present invention, in step 1, the carbon source is selected from organic substances containing carbon elements.
In a further preferred embodiment, in step 1, the carbon source is selected from the group consisting of-COOH and/or-COONa containing organics, such as-COONa containing organics.
In a still further preferred embodiment, in step 1, the carbon source is selected from one or more of sodium citrate, citric acid, oxalic acid and sodium oxalate, such as sodium citrate.
Wherein, carbon source is used as main raw material, and the carbon quantum dots are formed by a bottom-up method. The inventors have found through extensive experiments that an organic substance containing-COOH and/or-COONa is more effective, and more preferably an organic substance containing-COONa is most effective.
According to a preferred embodiment of the present invention, in step 1, the nitrogen source is selected from nitrogen-containing organic substances.
In a further preferred embodiment, in step 1, the nitrogen source is selected from ethylenediamine and/or urea, such as urea.
Wherein the nitrogen source provides nitrogen for the formation of carbon quantum dots.
According to a preferred embodiment of the present invention, in step 1, the cobalt source is selected from cobalt ion salts.
In a further preferred embodiment, in step 1, the cobalt source is selected from one or more of cobalt chloride, cobalt sulfate, cobalt nitrate and cobalt acetate, such as cobalt chloride.
The inventor finds that the carbon quantum dots prepared by doping cobalt ions can obviously improve the fluorescence quantum yield of the carbon quantum dots through a large number of experiments. The fluorescence quantum yield of the cobalt ion doped carbon quantum dot can reach 93%. And the obtained cobalt ion doped carbon quantum dots can stably emit yellow light, while the carbon quantum dots in the prior art generally emit blue light, but the blue light is not striking, and especially when the cobalt ion doped carbon quantum dots are applied to organisms, cell tissues emit blue light during cell imaging, so that the cobalt ion doped carbon quantum dots are difficult to distinguish.
According to a preferred embodiment of the present invention, the organic solvent is selected from one or more of benzene, toluene, xylene, ethanol, tetrahydrofuran and carbon tetrachloride.
In a further preferred embodiment, the organic solvent is selected from one or more of benzene, toluene and xylene.
In a still further preferred embodiment, the organic solvent is toluene.
In the present invention, the solvothermal reaction is carried out using an organic solvent as a solvent system, and the organic solvent used after the reaction is completed can be recovered and reused.
The inventor finds through a large number of experiments that (1) compared with a hydrothermal method, the method has higher fluorescence quantum yield which reaches 93%; (2) compared with a hydrothermal method, the cobalt ions obtained by the method are more suitable for industrial application and are beneficial to being used in textile industry and other industries, and the obtained carbon quantum dots have better compatibility and wider industrial application range.
According to a preferred embodiment of the present invention, the molar concentration of the carbon source in the precursor solution is 0.01-10 mol/L.
In a further preferred embodiment, the molar concentration of the carbon source in the precursor solution is 0.01-1 mol/L.
In a further preferred embodiment, the molar concentration of the carbon source in the precursor solution is 0.05-0.5mol/L, such as 0.05-0.2 mol/L.
Wherein the molar concentration of the carbon source is based on the molar concentration of carbon source molecules.
According to a preferred embodiment of the present invention, the molar concentration of the nitrogen source in the precursor solution is 0.01 to 10 mol/L.
In a further preferred embodiment, the molar concentration of the nitrogen source in the precursor solution is between 0.02 and 2 mol/L.
In a further preferred embodiment, the molar concentration of the nitrogen source in the precursor solution is 0.05-0.5mol/L, such as 0.1-0.3 mol/L.
Wherein the molar concentration of the nitrogen source is based on the molar concentration of nitrogen source molecules.
In a further preferred embodiment of the present invention, the molar concentration of the cobalt source in the precursor solution is 0.05-10 mol/L.
In a further preferred embodiment, the molar concentration of the cobalt source in the precursor solution is 0.05-0.5 mol/L.
In a still further preferred embodiment, the molar concentration of the cobalt source in the precursor solution is in the range of 0.05-0.2mol/L, such as 0.1 mol/L.
Wherein the molar concentration of the cobalt source is a molar concentration of cobalt ions therein. The inventors have found through extensive experiments that the fluorescence property increases with an increase in the amount of cobalt doped, but the fluorescence property does not further increase with an increase in the amount of cobalt doped, and therefore, the inventors limited the above amount.
And 2, placing the precursor solution obtained in the step 1 into a reaction kettle for reaction, and preferably cooling after the reaction is finished.
According to a preferred embodiment of the invention, in step 2, the reaction is carried out as follows: the reaction is carried out for 5 to 50 hours at a temperature of between 100 and 500 ℃.
In a further preferred embodiment, in step 2, the reaction is carried out as follows: the reaction is carried out for 8 to 24 hours at a temperature of between 150 and 300 ℃.
In a still further preferred embodiment, in step 2, the reaction is carried out as follows: the reaction is carried out at 180-220 ℃ for 10-14 h, for example, at 200 ℃ for 12 h.
In a preferred embodiment of the present invention, in step 2, the reaction is preferably cooled naturally after the end of the reaction.
And 3, carrying out post-treatment to obtain the cobalt ion doped carbon quantum dots.
According to a preferred embodiment of the invention, in step 3, the post-treatment comprises separation and drying.
In a further preferred embodiment, the separation is performed using a cylindrical membrane separation filter or centrifugation.
In a further preferred embodiment, the drying is vacuum freeze drying or vacuum heat drying, preferably vacuum freeze drying.
On the other hand, the invention discloses the cobalt ion doped carbon quantum dot obtained by the preparation method of the first aspect of the invention.
According to a preferred embodiment of the present invention, the cobalt ion-doped carbon quantum dots have an average particle size of 2 to 10 nm.
In a further preferred embodiment, the cobalt ion-doped carbon quantum dots have an average particle size of 2 to 8 nm.
According to a preferred embodiment of the present invention, the cobalt ion-doped carbon quantum dots have a fluorescence quantum yield of 85% or more.
In a further preferred embodiment, the cobalt ion-doped carbon quantum dots have a fluorescence quantum yield of 90% or more, for example 93%.
The cobalt ion doped carbon quantum dot obtained by the method has very high fluorescence quantum yield which is more than 85%, particularly more than 90%, and can reach 93% at most. However, in the prior art, the fluorescence quantum yield of the carbon quantum dots is generally about 10%, and even if the fluorescence quantum yield is improved by doping with other elements, the fluorescence quantum yield can only reach 30%.
According to a preferred embodiment of the present invention, the cobalt ion-doped carbon quantum dot emits orange-yellow fluorescence under the excitation of ultraviolet light.
The cobalt ion doped carbon quantum dot has high orange-yellow fluorescence intensity, which is obviously different from a common carbon quantum dot.
In a third aspect of the present invention, there is provided a cobalt ion-doped carbon quantum dot prepared by the preparation method according to the first aspect of the present invention or an application of the cobalt ion-doped carbon quantum dot according to the second aspect of the present invention.
According to a preferred embodiment of the invention, the cobalt ion doped carbon quantum dots are used as fluorescent probes for analyzing and detecting the water content in the organic solvent.
The inventor finds that the cobalt ion doped carbon quantum dot has good fluorescence performance in an organic solvent, but the fluorescence intensity is obviously quenched after a small amount of water is added, so that the cobalt ion doped carbon quantum dot can be used for detecting the water content in the organic solvent.
In a further preferred embodiment, the detection limit of the cobalt ion doped carbon quantum dots on the water content in the organic solvent can reach 0.01%.
The cobalt ion doped carbon quantum dot has a very low detection limit on the water content in the organic solvent, and can realize the detection of the organic solvent analysis level. In addition, the method is successfully used for detecting the water content in organic solvents such as ethanol, acetonitrile, tetrahydrofuran, dioxane and the like, and the detection limit can reach 0.01%.
The invention has the advantages that:
(1) the preparation method is simple, does not need to introduce complex functional groups for organic reaction, is only simple mixing heating reaction, and is suitable for industrial application;
(2) when the preparation method is applied to industrial application, the organic solvent can be recycled;
(3) the cobalt ion doped carbon quantum dot has a small particle size, and the average particle size is 2-10 nm;
(4) the cobalt ion doped carbon quantum dot has very high fluorescence quantum yield which is more than 85%, particularly more than 90%, such as 93%, and is obviously different from the prior art;
(5) the cobalt ion doped carbon quantum dot of the invention emits orange-yellow fluorescence under ultraviolet light;
(6) the cobalt ion doped carbon quantum dot can be used as a fluorescent probe for analyzing and detecting the water content in an organic solvent, the detection limit can reach 0.01 percent, and the analytical detection of the organic solvent is realized.
Examples
The invention is further described below by means of specific examples. However, these examples are only illustrative and do not limit the scope of the present invention.
Example 1
0.5882g of sodium citrate, 0.2404g of urea, 0.2588g of cobalt chloride and 20mL of toluene are mixed to form a precursor solution;
putting the obtained precursor solution into a 50mL stainless steel autoclave with a polytetrafluoroethylene lining, sealing, reacting for 12h at the temperature of 200 ℃, and naturally cooling a synthesized product;
separating the product by a cylindrical membrane separation filter, and drying the product in vacuum at the temperature of minus 50 ℃ to obtain cobalt ion doped carbon quantum dot powder.
The obtained cobalt ion-doped carbon quantum dot powder was redispersed with toluene, and the fluorescence quantum yield was found to be 91.0%.
Example 2
Mixing 0.134g of sodium oxalate, 0.2404g of urea, 0.2588g of cobalt chloride and 20mL of toluene to form a precursor solution;
putting the obtained precursor solution into a 50mL stainless steel autoclave with a polytetrafluoroethylene lining, sealing, reacting for 12h at the temperature of 200 ℃, and naturally cooling a synthesized product;
separating the product by a cylindrical membrane separation filter, and drying the product in vacuum at the temperature of minus 50 ℃ to obtain cobalt ion doped carbon quantum dot powder.
The obtained cobalt ion-doped carbon quantum dot powder was redispersed with toluene, and the fluorescence quantum yield was found to be 93%.
Example 3
0.5882g of citric acid, 0.1202g of urea, 0.1295g of cobalt chloride and 20mL of toluene are mixed to form a precursor solution;
putting the obtained precursor solution into a 50mL stainless steel autoclave with a polytetrafluoroethylene lining, sealing, reacting for 14h at 180 ℃, and naturally cooling a synthesized product;
separating the product by a cylindrical membrane separation filter, and drying the product in vacuum at the temperature of minus 50 ℃ to obtain cobalt ion doped carbon quantum dot powder.
The obtained cobalt ion-doped carbon quantum dot powder was redispersed with toluene, and the fluorescence quantum yield was found to be 88.5%.
Example 4
0.2701g of oxalic acid, 0.3606g of ethylenediamine, 0.8732g of cobalt nitrate hexahydrate and 20mL of toluene are mixed to form a precursor solution;
putting the obtained precursor solution into a 50mL stainless steel autoclave with a polytetrafluoroethylene lining, sealing, reacting for 10h at 220 ℃, and naturally cooling a synthesized product;
separating the product by a cylindrical membrane separation filter, and drying the product in vacuum at 50 ℃ to obtain cobalt ion doped carbon quantum dot powder.
The obtained cobalt ion-doped carbon quantum dot powder was redispersed with toluene, and the fluorescence quantum yield was found to be 89.8%.
Example 5
1.1764g of sodium citrate, 0.4808g of urea, 0.7083g of cobalt acetate and 20mL of toluene are mixed to form a precursor solution;
placing the obtained precursor solution into a 50mL stainless steel autoclave with a polytetrafluoroethylene lining, sealing, reacting for 16h at the temperature of 300 ℃, and naturally cooling a synthesized product;
separating the product by a cylindrical membrane separation filter, and drying the product in vacuum at the temperature of minus 50 ℃ to obtain cobalt ion doped carbon quantum dot powder.
The obtained cobalt ion-doped carbon quantum dot powder was redispersed with toluene, and the fluorescence quantum yield was measured to be 90.6%.
Comparative example
Comparative example 1
The procedure of example 1 was repeated except that: no cobalt source was added, i.e. no cobalt ion doping was performed.
The obtained cobalt ion-undoped carbon-doped quantum dot powder was redispersed with toluene, and the fluorescence quantum yield was found to be 23%.
Comparative example 2
The procedure of example 1 was repeated except that: water is used as a solvent to replace toluene.
The obtained cobalt ion-doped carbon quantum dot powder was redispersed with water (since it was insoluble in an organic solvent), and the fluorescence quantum yield was measured to be 30%.
Examples of the experiments
Experimental example 1 Transmission Electron microscopy
The cobalt ion-doped carbon quantum dot obtained in example 1 was subjected to transmission electron microscopy, and the structure is shown in fig. 1.
As can be seen from FIG. 1, the cobalt ion doped carbon quantum dots obtained by the method have a small particle size, and specifically, the average particle size is 2-10 nm.
Experimental example 2 detection of fluorescence Properties
(1) Fluorescence performance detection is performed on the cobalt ion-doped carbon quantum dots obtained in example 1, and a three-dimensional fluorescence spectrum and a corresponding color spectrum are obtained, as shown in fig. 2a and fig. 2b, respectively. From fig. 2a and 2b, it can be seen that the optimal excitation wavelength of the cobalt ion-doped carbon quantum dots is 440nm, and the optimal emission wavelength is 565nm (yellow light);
two cobalt ion-doped carbon quanta obtained in example 1 were dissolved in toluene and irradiated under natural light (left side) and ultraviolet light with a wavelength of 365nm (right side), and as a result, as shown in fig. 2c, it can be seen from fig. 2c that the prepared cobalt ion carbon quanta point is a bright yellow transparent liquid (left side) under natural light, and emits orange yellow fluorescence (right side) under ultraviolet light with a wavelength of 365nm, which is consistent with the results of fig. 2a to 2 b.
(2) Fluorescence performance detection is performed on the cobalt-carbon-undoped quantum dots obtained in the comparative example 1, and a three-dimensional fluorescence spectrum and a corresponding color spectrum diagram are obtained, which are respectively shown in fig. 3a and fig. 3 b. As shown in fig. 3a and 3b, it can be seen that the emission wavelength of the cobalt-free carbon quantum dots depends on the length of the excitation wave, and has excitation wavelength dependence;
two cobalt-carbon non-doped quantum dots obtained in comparative example 1 were dissolved in toluene and irradiated under natural light (left side) and ultraviolet light with a wavelength of 365nm (right side), and as a result, as shown in fig. 3c, it can be seen from fig. 3c that the prepared cobalt ion carbon quantum dots are light yellow transparent liquid (left side) under natural light, and emit very weak light blue fluorescence (right side) under ultraviolet light with a wavelength of 365 nm.
(3) Fluorescence performance detection is performed on the cobalt ion-doped carbon quantum dots obtained in comparative example 2 (hydrothermal method), and a three-dimensional fluorescence spectrum and a corresponding color spectrum are obtained, as shown in fig. 4a and 4 b. As shown in fig. 4a and 4b, it can be seen that the optimal emission wavelength is around 440nm (weak blue light);
two cobalt ion-doped carbon quanta obtained in comparative example 2 were dissolved in water (because they are not dissolved in toluene) and irradiated under natural light (left side) and ultraviolet light with a wavelength of 365nm (right side), and as shown in fig. 4c, it can be seen from fig. 4c that the prepared cobalt ion-doped carbon quanta is reddish brown liquid (left side) under natural light and emits blue fluorescence (right side) under ultraviolet light with a wavelength of 365 nm.
Experimental example 3XPS detection
The results of XPS detection on the cobalt ion-doped carbon quantum dots obtained in example 1 are shown in fig. 5, where there are 4 peaks at 284.76eV, 400.22eV, 532.24eV, and 785.98eV, which correspond to the binding energies of C1s, N1s, O1s, and Co2p, respectively, which indicates that the cobalt ion-doped carbon quantum dots prepared by the method of the present invention contain C, N, O and Co, which correspond to 77.45%, 4.55%, 17.73%, and 0.26%, respectively.
Experimental example 4 fluorescence Quantum yield assay
The cobalt ion-doped carbon quantum dots obtained in example 1 were subjected to fluorescence quantum yield detection, which is the absolute fluorescence quantum yield measured by an integrating sphere, and the result is shown in fig. 6a, wherein the reference curve represents a solvent peak, i.e., a background peak. The cobalt ion-doped carbon quantum dot obtained in example 1 had a fluorescence Quantum Yield (QY) of 91.0%.
Similarly, the fluorescence quantum yields of examples 2 to 5 were measured to be 93%, 88.5%, 89.8% and 90.6%, respectively.
The fluorescence quantum yields of the products obtained in comparative examples 1 to 2 were measured and were 23% and 27% as shown in fig. 6b and 6c, respectively.
Experimental example 5 detection of Water content in organic solvent
The cobalt ion doped carbon quantum dots obtained in example 1 were used for detecting the water content in the organic solvent:
(1) the fluorescence property of the cobalt ion doped carbon quantum dots in a dioxane solution with the water content of 10% is detected, the result is shown in fig. 7, a graph showing the change of the intensity of fluorescence emitted at 565nm (440nm excitation) along with time shows that the fluorescence intensity does not change after 1 minute, so that the detection time can be set to 1 minute, and the rapid detection of the water content in the organic solvent is realized. (2) The fluorescence performance of the cobalt ion-doped carbon quantum dots in dioxane solutions with different water contents (0-10%) after 1 minute is detected, and the result is shown in fig. 8 (wherein, from top to bottom, the water contents (V/V,%) are 0, 0.01, 0.4, 0.8, 2, 4, 6, 8, 10 in sequence), fig. 8 shows the fluorescence emission spectrum (excitation wavelength is 440nm) of the cobalt ion-doped carbon quantum dots in dioxane solutions with different water contents after 1 minute, and as can be seen from fig. 8, the fluorescence of the cobalt ion-doped carbon quantum dots is gradually quenched with the increase of the water contents.
The correspondence between fluorescence quenching and different water contents in fig. 8 can be summarized as shown in fig. 9.
(3) The results obtained in (2) above (shown in FIGS. 8 and 9) were linearly expressed, and as shown in FIG. 10, it was found that the fluorescence quenching value and the water content exhibited a very good linear relationship between the water content of 0% and 2%, where R is a linear relationship20.9963, the detection limit can reach 0.01%, and the detection of an analysis level can be realized.
The invention has been described in detail with reference to the preferred embodiments and illustrative examples. It should be noted, however, that these specific embodiments are only illustrative of the present invention and do not limit the scope of the present invention in any way. Various modifications, equivalent substitutions and alterations can be made to the technical content and embodiments of the present invention without departing from the spirit and scope of the present invention, and these are within the scope of the present invention. The scope of the invention is defined by the appended claims.
Claims (8)
1. A preparation method of cobalt ion doped carbon quantum dots is characterized by comprising the following steps:
step 1, adding a carbon source, a nitrogen source and a cobalt source into an organic solvent to form a precursor solution, wherein the organic solvent is selected from one of benzene, toluene and xylene;
step 2, placing the precursor solution obtained in the step 1 into a reaction kettle for reaction, and cooling after the reaction is finished;
step 3, carrying out post-treatment to obtain cobalt ion doped carbon quantum dots;
in the precursor solution, the precursor solution is added with a solvent,
the carbon source is selected from organic matters containing-COONa, and the molar concentration is 0.05-0.5 mol/L;
the nitrogen source is selected from ethylenediamine and/or urea, and the molar concentration is 0.05-0.5 mol/L;
the cobalt source is selected from one of cobalt chloride, cobalt sulfate, cobalt nitrate and cobalt acetate, and the molar concentration is 0.05-0.2 mol/L;
in step 2, the reaction proceeds as follows: performing the reaction for 10 to 14 hours at the temperature of between 180 and 220 ℃; and
in step 3, the post-treatment includes separation using a cylindrical membrane separation filter or centrifugation and drying.
2. The method according to claim 1, wherein in step 1, the carbon source is selected from sodium citrate or sodium oxalate.
3. The method according to claim 1, wherein the nitrogen source is urea in step 1.
4. The method according to claim 1, wherein in step 1, the cobalt source is cobalt chloride.
5. The method according to claim 1, wherein the organic solvent is toluene in step 1.
6. The production method according to one of claims 1 to 5, characterized in that, in the precursor solution,
the molar concentration of the carbon source is 0.05-0.2 mol/L;
the molar concentration of the nitrogen source is 0.1-0.3 mol/L; and
the molar concentration of the cobalt source is 0.1 mol/L.
7. The production method according to claim 1,
in step 2, the reaction proceeds as follows: reacting for 12 hours at 200 ℃; and/or
In step 3, the post-treatment includes separation using a cylindrical membrane separation filter or centrifugation and drying.
8. The production method according to claim 7,
in step 2, the reaction proceeds as follows: reacting for 12 hours at 200 ℃;
in step 3, the drying is vacuum freeze drying or vacuum heat drying.
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| CN109221104B (en) * | 2018-10-18 | 2022-03-22 | 河南师范大学 | Silver-containing carbon dots, preparation method and application thereof |
| CN109777411B (en) * | 2019-03-20 | 2022-04-12 | 南宁师范大学 | Preparation method of cobalt-doped magnetic carbon quantum dots and method for detecting uric acid |
| CN110132917B (en) * | 2019-05-14 | 2021-07-27 | 山西大学 | Cobalt-nitrogen double-doped carbon dot and preparation method and application thereof |
| CN110669518A (en) * | 2019-11-21 | 2020-01-10 | 中国林业科学研究院林产化学工业研究所 | Fluorescent carbon dot and preparation method and application thereof |
| CN112852417B (en) * | 2020-12-31 | 2023-07-07 | 苏州国纳思新材料科技有限公司 | Preparation method of metal ion doped carbon dot solution |
| CN115627167B (en) * | 2022-09-15 | 2023-10-13 | 西北工业大学 | N, B co-doped carbon dot for simultaneously detecting multiple metal ions and preparation method thereof |
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