CN110669518A - Fluorescent carbon dot and preparation method and application thereof - Google Patents

Fluorescent carbon dot and preparation method and application thereof Download PDF

Info

Publication number
CN110669518A
CN110669518A CN201911146744.3A CN201911146744A CN110669518A CN 110669518 A CN110669518 A CN 110669518A CN 201911146744 A CN201911146744 A CN 201911146744A CN 110669518 A CN110669518 A CN 110669518A
Authority
CN
China
Prior art keywords
carbon dots
gallic acid
fluorescent carbon
fluorescent
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201911146744.3A
Other languages
Chinese (zh)
Inventor
许利娜
夏建陵
李梅
丁海阳
李守海
杨小华
张燕
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Chemical Industry of Forest Products of CAF
Original Assignee
Institute of Chemical Industry of Forest Products of CAF
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Chemical Industry of Forest Products of CAF filed Critical Institute of Chemical Industry of Forest Products of CAF
Priority to CN201911146744.3A priority Critical patent/CN110669518A/en
Publication of CN110669518A publication Critical patent/CN110669518A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
    • C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00—Manufacture or treatment of nanostructures
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
    • C01B32/00—Carbon; Compounds thereof
    • C01B32/15—Nano-sized carbon materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Materials Engineering (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Inorganic Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Manufacturing & Machinery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

A fluorescent carbon dot and a preparation method and application thereof. The preparation method comprises the following steps: 1) weighing a certain amount of gallic acid and an organic solvent, adding the gallic acid and the organic solvent into a reaction kettle with a polytetrafluoroethylene inner container, reacting for 12-48 h at 160-220 ℃, cooling to room temperature after the reaction is finished, filtering the obtained brown yellow liquid, and freeze-drying the obtained liquid to obtain the fluorescent carbon dots. The carbon dots can be used as fluorescent probes for detecting water content in organic solvents and for resisting oxidation activity and removing active oxygen free radicals. The gallic acid used as the raw material is a polyphenol compound widely existing in nature, and the preparation method of the carbon dots is simple, so that the gallic acid can be used for detecting water and has antioxidant activity.

Description

Fluorescent carbon dot and preparation method and application thereof
Technical Field
The invention belongs to the field of fluorescence detection, and particularly relates to a fluorescent carbon dot capable of detecting water and having oxidation resistance, and a preparation method and application thereof.
Background
The fluorescent carbon material is a novel fluorescent material developed in recent years, has the advantages of good biocompatibility, low toxicity, good light stability and the like, and is widely applied to the fields of detection, cell imaging, drug carriers and the like. Moisture content can affect the storage of food and pharmaceutical products. Trace amounts of water also cause corrosion and serious damage to industrial facilities and mechanical equipment, and thus an effective method for detecting the water content is required. Consistent with medical science and health care science, excessive free radicals oxidize biomacromolecules, so that tissue damage is caused, and a series of diseases such as aging, arteriosclerosis, cardiovascular diseases, nervous system abnormality and the like of a human body are caused.
Gallic acid is a chemical component of natural products, widely exists in Rheum palmatum, Eucalyptus robusta, Corni fructus, Coriaria sinica, Sapium sebiferum, pomegranate, acacia, etc., and is a polyphenol compound existing in nature. The fluorescent carbon dots which can be used for detecting water and have oxidation resistance are prepared by taking natural biomass gallic acid as a raw material through a simple method.
Disclosure of Invention
The technical problem to be solved is as follows: the invention provides a fluorescent carbon dot capable of detecting water and having oxidation resistance, a preparation method and application thereof, wherein the fluorescent carbon dot is prepared from biomass gallic acid with low cost.
The technical scheme is as follows: a preparation method of a fluorescent carbon dot comprises the following steps: weighing gallic acid and an organic solvent, adding the gallic acid and the organic solvent into a hydrothermal reaction kettle with a polytetrafluoroethylene inner container, wherein the mass-to-volume ratio (g/mL) of the gallic acid to the organic solvent is (0.1-2): 30, reacting at 150-220 ℃ for 12-48 h to obtain brown yellow liquid, filtering, and freeze-drying the filtrate to obtain the fluorescent carbon dots.
Preferably, the organic solvent is ethanol, dimethyl sulfoxide, dimethylformamide or methylpyrrolidone.
Preferably, the ratio of the gallic acid to the organic solvent by mass/volume (g/mL) is 0.1: 30.
The fluorescent carbon dot prepared by any one of the preparation methods.
The fluorescent carbon dots are applied to the preparation of a fluorescent probe for detecting the water content.
A fluorescent probe for detecting water content is prepared from the fluorescent carbon dots.
The fluorescent carbon dots are applied to preparation of products with antioxidant activity and active oxygen free radical scavenging.
A product with antioxidant activity and scavenging active oxygen free radical is prepared from the fluorescent carbon dots.
Has the advantages that: 1) the invention provides a method for preparing a fluorescent carbon dot capable of being used for detecting water content by using biomass gallic acid. 2) The prepared carbon dots have antioxidant activity and active oxygen free radical scavenging capacity. 3) The raw material gallic acid has low price, the preparation process is simple and convenient, and the large-scale production is easy to realize. 4) The invention has great significance for high-value utilization of biomass resources.
Drawings
FIG. 1 is a chart of the UV-VIS absorption spectrum of carbon dots prepared according to example 2;
FIG. 2 is a fluorescence spectrum of a carbon dot prepared according to example 2;
FIG. 3 is a graph of the scavenging capacity of free radicals for carbon dots prepared according to example 2 (carbon dot concentration from left to right: 0, 0.02, 0.04, 0.06, 0.08, 0.10 mg/mL);
figure 4 is a carbon point ir spectrum prepared according to example 2.
Detailed Description
The present invention is described in detail below by way of examples, which give detailed embodiments and specific operating procedures for further illustration of the invention and are not to be construed as limiting the scope of the invention.
Example 1
Preparation of fluorescent carbon dots: weighing 0.1 g of gallic acid and 30 mL of absolute ethyl alcohol, adding the gallic acid and the absolute ethyl alcohol into a 50 mL reaction kettle with a polytetrafluoroethylene inner container, reacting at 180 ℃ for 12 h, cooling to room temperature after the reaction is finished, filtering the obtained solution by using a microporous filter membrane (0.22 mu m), and freeze-drying the obtained brown liquid to obtain carbon dots.
Application of carbon dots in detection of water content in ethanol: uniformly dispersing the prepared carbon dots into absolute ethyl alcohol, respectively adding 3mL of carbon dot solution and a series of distilled water with different volumes into a 5 mL fluorescence cuvette, uniformly mixing to ensure that the final water content in the solution is 0-10% (v/v), and measuring the fluorescence property of each solution by using a fluorescence spectrometer to obtain a fluorescence spectrogram.
Total phenol content of carbon dots: the total phenol content of the carbon dots is detected by a Folin-Ciocalteu (FC) method, 20 muL of gallic acid aqueous solution of 0, 50, 100, 150, 250, 500, 1000, 1500 and 2000 mg/L are respectively sucked and placed in a disposable sample tube, 1.58 m L water and 100 muL of Folin-Ciocalteu reagent are respectively added and mixed uniformly. And waiting for 30s, adding 300 mu L of saturated sodium carbonate solution, shaking up, standing at room temperature in a dark place for 2h, measuring the absorbance at 765 nm by using an ultraviolet visible spectrophotometer, and drawing a standard curve of concentration and absorbance. At the same time, the carbon dots were mixed with distilled water (100 mL), then 4 mL was pipetted into a 50 mL volumetric flask with 3mL Folin-Ciocalteu reagent and 30 mL of distilled water, and 10 mL of saturated Na was added2CO3The mixture was added to a solvent, left in the dark at room temperature for 2 hours, and the absorbance of the sample was measured at 765 nm and used to calculate the phenol content in the carbon dots.
Scavenging ability of free radicals at carbon sites: detection by DPPH methodFree radical scavenging ability of carbon dots, carbon dots were added to 3mL of 0.04 mg-1The absorbance at 515 nm was then measured in ethanol DPPH solution and its clearance of DPPH Radicals (RSA) was calculated using the formula: RSA (%) = (a)C-A0）/ ACX 100% where ACIs the absorbance of DPPH at 515 nm in the presence of carbon spots, A0Is the absorbance in the absence of carbon dots.
Example 2
Preparation of fluorescent carbon dots: weighing 0.1 g of gallic acid and 30 mL of absolute ethyl alcohol, adding into a 50 mL reaction kettle with a polytetrafluoroethylene inner container, reacting at 180 ℃ for 24 h, cooling to room temperature after the reaction is finished, filtering the obtained solution with a microporous filter membrane, and freeze-drying the obtained brown liquid to obtain carbon dots.
Application of carbon dots in detection of water content in ethanol: uniformly dispersing the prepared carbon dots into absolute ethyl alcohol, respectively adding 3mL of carbon dot solution and a series of distilled water with different volumes into a 5 mL fluorescence cuvette, uniformly mixing to ensure that the final water content in the solution is 0-10% (v/v), and measuring the fluorescence property of each solution by using a fluorescence spectrometer to obtain a fluorescence spectrogram (figure 2).
Total phenol content of carbon dots: the total phenol content of the carbon dots is detected by a Folin-Ciocalteu (FC) method, 20 muL of gallic acid aqueous solution of 0, 50, 100, 150, 250, 500, 1000, 1500 and 2000 mg/L are respectively sucked and placed in a disposable sample tube, 1.58 m L water and 100 muL of Folin-Ciocalteu reagent are respectively added and mixed uniformly. And waiting for 30s, adding 300 mu L of saturated sodium carbonate solution, shaking up, standing at room temperature in a dark place for 2h, measuring the absorbance at 765 nm by using an ultraviolet visible spectrophotometer, and drawing a standard curve of concentration and absorbance. At the same time, the carbon dots were mixed with distilled water (100 mL), then 4 mL was pipetted into a 50 mL volumetric flask with 3mL Folin-Ciocalteu reagent and 30 mL of distilled water, and 10 mL of saturated Na was added2CO3The mixture was added to a solvent, left in the dark at room temperature for 2 hours, and the absorbance of the sample was measured at 765 nm and used to calculate the phenol content in the carbon dots.
Free radical of carbon pointThe cleaning ability of (c): the radical scavenging ability of the carbon dots was examined by DPPH method, and 3mL of 0.04 mg of the carbon dots were added-1The absorbance at 515 nm was then measured in ethanol DPPH solution and its clearance of DPPH Radicals (RSA) was calculated using the formula: RSA (%) = (a)C-A0）/ ACX 100% where ACIs the absorbance of DPPH at 515 nm in the presence of carbon spots, A0Is the absorbance in the absence of carbon dots.
Example 3
Preparation of fluorescent carbon dots: weighing 0.1 g of gallic acid and 30 mL of absolute ethyl alcohol, adding into a 50 mL reaction kettle with a polytetrafluoroethylene inner container, reacting at 160 ℃ for 12 h, cooling to room temperature after the reaction is finished, filtering the obtained solution with a microporous filter membrane, and freeze-drying the obtained brown liquid to obtain carbon dots.
Application of carbon dots in detection of water content in ethanol: uniformly dispersing the prepared carbon dots into absolute ethyl alcohol, respectively adding 3mL of carbon dot solution and a series of distilled water with different volumes into a 5 mL fluorescence cuvette, uniformly mixing to ensure that the final water content in the solution is 0-10% (v/v), and measuring the fluorescence property of each solution by using a fluorescence spectrometer to obtain a fluorescence spectrogram.
Total phenol content of carbon dots: the total phenol content of the carbon dots is detected by a Folin-Ciocalteu (FC) method, 20 muL of gallic acid aqueous solution of 0, 50, 100, 150, 250, 500, 1000, 1500 and 2000 mg/L are respectively sucked and placed in a disposable sample tube, 1.58 m L water and 100 muL of Folin-Ciocalteu reagent are respectively added and mixed uniformly. And waiting for 30s, adding 300 mu L of saturated sodium carbonate solution, shaking up, standing at room temperature in a dark place for 2h, measuring the absorbance at 765 nm by using an ultraviolet visible spectrophotometer, and drawing a standard curve of concentration and absorbance. At the same time, the carbon dots were mixed with distilled water (100 mL), then 4 mL was pipetted into a 50 mL volumetric flask with 3mL Folin-Ciocalteu reagent and 30 mL of distilled water, and 10 mL of saturated Na was added2CO3The mixture was added to a solvent, left in the dark at room temperature for 2 hours, and the absorbance of the sample was measured at 765 nm and used to calculate the phenol content in the carbon dots.
Scavenging ability of free radicals at carbon sites: the radical scavenging ability of the carbon dots was examined by DPPH method, and 3mL of 0.04 mg of the carbon dots were added-1The absorbance at 515 nm was then measured in ethanol DPPH solution and its clearance of DPPH Radicals (RSA) was calculated using the formula: RSA (%) = (a)C-A0）/ AC X 100% where ACIs the absorbance of DPPH at 515 nm in the presence of carbon spots, A0Is the absorbance in the absence of carbon dots.
Example 4
Preparation of fluorescent carbon dots: weighing 0.5 g of gallic acid and 30 mL of absolute ethyl alcohol, adding into a 50 mL reaction kettle with a polytetrafluoroethylene inner container, reacting at 180 ℃ for 24 h, cooling to room temperature after the reaction is finished, filtering the obtained solution with a microporous filter membrane, and freeze-drying the obtained brown liquid to obtain carbon dots.
Application of carbon dots in detection of water content in ethanol: uniformly dispersing the prepared carbon dots into absolute ethyl alcohol, respectively adding 3mL of carbon dot solution and a series of distilled water with different volumes into a 5 mL fluorescence cuvette, uniformly mixing to ensure that the final water content in the solution is 0-10% (v/v), and measuring the fluorescence property of each solution by using a fluorescence spectrometer to obtain a fluorescence spectrogram.
Total phenol content of carbon dots: the total phenol content of the carbon dots is detected by a Folin-Ciocalteu (FC) method, 20 muL of gallic acid aqueous solution of 0, 50, 100, 150, 250, 500, 1000, 1500 and 2000 mg/L are respectively sucked and placed in a disposable sample tube, 1.58 m L water and 100 muL of Folin-Ciocalteu reagent are respectively added and mixed uniformly. And waiting for 30s, adding 300 mu L of saturated sodium carbonate solution, shaking up, standing at room temperature in a dark place for 2h, measuring the absorbance at 765 nm by using an ultraviolet visible spectrophotometer, and drawing a standard curve of concentration and absorbance. At the same time, the carbon dots were mixed with distilled water (100 mL), then 4 mL was pipetted into a 50 mL volumetric flask with 3mL Folin-Ciocalteu reagent and 30 mL of distilled water, and 10 mL of saturated Na was added2CO3Adding into solvent, standing at room temperature in dark place for 2 hr, measuring absorbance of sample at 765 nm,and used to calculate the phenol content in the carbon dots.
Scavenging ability of free radicals at carbon sites: the radical scavenging ability of the carbon dots was examined by DPPH method, and 3mL of 0.04 mg of the carbon dots were added-1The absorbance at 515 nm was then measured in ethanol DPPH solution and its clearance of DPPH Radicals (RSA) was calculated using the formula: RSA (%) = (a)C-A0）/ AC X 100% where ACIs the absorbance of DPPH at 515 nm in the presence of carbon spots, A0Is the absorbance in the absence of carbon dots.
Example 5
Preparation of fluorescent carbon dots: weighing 0.1 g of gallic acid and 30 mL of anhydrous methyl pyrrolidone, adding into a 50 mL reaction kettle with a polytetrafluoroethylene inner container, reacting at 180 ℃ for 12 h, cooling to room temperature after the reaction is finished, filtering the obtained solution with a microporous filter membrane, and freeze-drying the obtained brown liquid to obtain carbon dots.
Application of carbon dots in detection of water content in ethanol: uniformly dispersing the prepared carbon dots into absolute ethyl alcohol, respectively adding 3mL of carbon dot solution and a series of distilled water with different volumes into a 5 mL fluorescence cuvette, uniformly mixing to ensure that the final water content in the solution is 0-10% (v/v), and measuring the fluorescence property of each solution by using a fluorescence spectrometer to obtain a fluorescence spectrogram.
Total phenol content of carbon dots: the total phenol content of the carbon dots is detected by a Folin-Ciocalteu (FC) method, 20 muL of gallic acid aqueous solution of 0, 50, 100, 150, 250, 500, 1000, 1500 and 2000 mg/L are respectively sucked and placed in a disposable sample tube, 1.58 m L water and 100 muL of Folin-Ciocalteu reagent are respectively added and mixed uniformly. And waiting for 30s, adding 300 mu L of saturated sodium carbonate solution, shaking up, standing at room temperature in a dark place for 2h, measuring the absorbance at 765 nm by using an ultraviolet visible spectrophotometer, and drawing a standard curve of concentration and absorbance. At the same time, the carbon dots were mixed with distilled water (100 mL), then 4 mL was pipetted into a 50 mL volumetric flask with 3mL Folin-Ciocalteu reagent and 30 mL of distilled water, and 10 mL of saturated Na was added2CO3Adding into solventThe sample was left at room temperature in the dark for 2 hours, and the absorbance at 765 nm was measured and used to calculate the phenol content in the carbon dots.
Scavenging ability of free radicals at carbon sites: the radical scavenging ability of the carbon dots was examined by DPPH method, and 3mL of 0.04 mg of the carbon dots were added-1The absorbance at 515 nm was then measured in ethanol DPPH solution and its clearance of DPPH Radicals (RSA) was calculated using the formula: RSA (%) = (a)C-A0）/ ACX 100% where ACIs the absorbance of DPPH at 515 nm in the presence of carbon spots, A0Is the absorbance in the absence of carbon dots.
Example 6
Preparation of fluorescent carbon dots: weighing 0.5 g of gallic acid and 30 mL of anhydrous methyl pyrrolidone, adding into a 50 mL reaction kettle with a polytetrafluoroethylene inner container, reacting at 180 ℃ for 24 h, cooling to room temperature after the reaction is finished, filtering the obtained solution with a microporous filter membrane, and freeze-drying the obtained brown liquid to obtain carbon dots.
Application of carbon dots in detection of water content in ethanol: uniformly dispersing the prepared carbon dots into absolute ethyl alcohol, respectively adding 3mL of carbon dot solution and a series of distilled water with different volumes into a 5 mL fluorescence cuvette, uniformly mixing to ensure that the final water content in the solution is 0-10% (v/v), and measuring the fluorescence property of each solution by using a fluorescence spectrometer to obtain a fluorescence spectrogram.
Total phenol content of carbon dots: the total phenol content of the carbon dots is detected by a Folin-Ciocalteu (FC) method, 20 muL of gallic acid aqueous solution of 0, 50, 100, 150, 250, 500, 1000, 1500 and 2000 mg/L are respectively sucked and placed in a disposable sample tube, 1.58 m L water and 100 muL of Folin-Ciocalteu reagent are respectively added and mixed uniformly. And waiting for 30s, adding 300 mu L of saturated sodium carbonate solution, shaking up, standing at room temperature in a dark place for 2h, measuring the absorbance at 765 nm by using an ultraviolet visible spectrophotometer, and drawing a standard curve of concentration and absorbance. At the same time, the carbon dots were mixed with distilled water (100 mL) and 4 mL was removed and added to 50 mL with 3mL Folin-Ciocalteu reagent and 30 mL of distilled waterIn the mL volumetric flask, 10 mL of saturated Na was added2CO3The mixture was added to a solvent, left in the dark at room temperature for 2 hours, and the absorbance of the sample was measured at 765 nm and used to calculate the phenol content in the carbon dots.
Scavenging ability of free radicals at carbon sites: the radical scavenging ability of the carbon dots was examined by DPPH method, and 3mL of 0.04 mg of the carbon dots were added-1The absorbance at 515 nm was then measured in ethanol DPPH solution and its clearance of DPPH Radicals (RSA) was calculated using the formula: RSA (%) = (a)C-A0）/ ACX 100% where ACIs the absorbance of DPPH at 515 nm in the presence of carbon spots, A0Is the absorbance in the absence of carbon dots.

Claims (8)

1. A preparation method of a fluorescent carbon dot is characterized by comprising the following steps: weighing gallic acid and an organic solvent, adding the gallic acid and the organic solvent into a hydrothermal reaction kettle with a polytetrafluoroethylene inner container, wherein the mass-to-volume ratio (g/mL) of the gallic acid to the organic solvent is (0.1-2): 30, reacting at 150-220 ℃ for 12-48 h to obtain brown yellow liquid, filtering, and freeze-drying the filtrate to obtain the fluorescent carbon dots.
2. The method for preparing a fluorescent carbon dot according to claim 1, wherein the organic solvent is ethanol, dimethyl sulfoxide, dimethylformamide or methylpyrrolidone.
3. The method for producing a fluorescent carbon dot according to claim 1, wherein the ratio of the gallic acid to the organic solvent in terms of mass to volume (g/mL) is 0.1: 30.
4. A fluorescent carbon dot produced by the production method according to any one of claims 1 to 3.
5. Use of the fluorescent carbon dot of claim 4 for preparing a fluorescent probe for detecting water content.
6. A fluorescent probe for detecting water content, characterized by being prepared from the fluorescent carbon dot as claimed in claim 4.
7. The use of the fluorescent carbon dot of claim 4 in the preparation of products with antioxidant activity and scavenging active oxygen free radicals.
8. An antioxidant active and active oxygen free radical scavenging product prepared from the fluorescent carbon dots of claim 4.
CN201911146744.3A 2019-11-21 2019-11-21 Fluorescent carbon dot and preparation method and application thereof Pending CN110669518A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911146744.3A CN110669518A (en) 2019-11-21 2019-11-21 Fluorescent carbon dot and preparation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911146744.3A CN110669518A (en) 2019-11-21 2019-11-21 Fluorescent carbon dot and preparation method and application thereof

Publications (1)

Publication Number Publication Date
CN110669518A true CN110669518A (en) 2020-01-10

Family

ID=69087984

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201911146744.3A Pending CN110669518A (en) 2019-11-21 2019-11-21 Fluorescent carbon dot and preparation method and application thereof

Country Status (1)

Country Link
CN (1) CN110669518A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111747397A (en) * 2020-06-30 2020-10-09 山西大学 Preparation method and application of grape carbon quantum dots
CN114225031A (en) * 2021-12-14 2022-03-25 贵州省烟草科学研究院 Tobacco-based carbon nanoenzyme and preparation method thereof
CN115058171A (en) * 2022-06-24 2022-09-16 中国铁道科学研究院集团有限公司金属及化学研究所 Low-surface-treatment water-based paint for station house steel structure and preparation method thereof
CN116942892A (en) * 2023-08-30 2023-10-27 南华大学附属第一医院 Hydrogel dressing for treating diabetes wound surface and preparation method thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105651749A (en) * 2016-01-19 2016-06-08 湖南科技大学 Method for detecting water content in tetrahydrofuran through carbon nanoparticles
CN108165268A (en) * 2018-01-19 2018-06-15 北京服装学院 A kind of preparation of copper ion doped carbon quantum dot and obtained carbon quantum dot and application
CN108251108A (en) * 2018-01-19 2018-07-06 北京服装学院 A kind of preparation of iron ion doping carbon quantum dot and obtained carbon quantum dot and application
CN108251107A (en) * 2018-01-19 2018-07-06 北京服装学院 A kind of preparation of ZnO thin film carbon quantum dot and obtained carbon quantum dot and application
CN108300462A (en) * 2018-01-19 2018-07-20 北京服装学院 A kind of preparation of calcium ion doping carbon quantum dot and obtained carbon quantum dot and application
CN108384537A (en) * 2018-01-19 2018-08-10 北京服装学院 A kind of preparation of barium ions doping carbon quantum dot and obtained carbon quantum dot and application
CN108998013A (en) * 2018-08-16 2018-12-14 西北大学 A kind of green fluorescence quantum dot and preparation method thereof and copper ion detection application

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105651749A (en) * 2016-01-19 2016-06-08 湖南科技大学 Method for detecting water content in tetrahydrofuran through carbon nanoparticles
CN108165268A (en) * 2018-01-19 2018-06-15 北京服装学院 A kind of preparation of copper ion doped carbon quantum dot and obtained carbon quantum dot and application
CN108251108A (en) * 2018-01-19 2018-07-06 北京服装学院 A kind of preparation of iron ion doping carbon quantum dot and obtained carbon quantum dot and application
CN108251107A (en) * 2018-01-19 2018-07-06 北京服装学院 A kind of preparation of ZnO thin film carbon quantum dot and obtained carbon quantum dot and application
CN108300462A (en) * 2018-01-19 2018-07-20 北京服装学院 A kind of preparation of calcium ion doping carbon quantum dot and obtained carbon quantum dot and application
CN108384537A (en) * 2018-01-19 2018-08-10 北京服装学院 A kind of preparation of barium ions doping carbon quantum dot and obtained carbon quantum dot and application
CN108998013A (en) * 2018-08-16 2018-12-14 西北大学 A kind of green fluorescence quantum dot and preparation method thereof and copper ion detection application

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
M. ZHENG ET AL.: "A carbon dots-based nanoprobe for intracellular Fe3+ detection", 《MATERIALS TODAY CHEMISTRY》 *
郑金贵编著: "《农产品品质学》", 31 December 2014, 厦门大学出版社 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111747397A (en) * 2020-06-30 2020-10-09 山西大学 Preparation method and application of grape carbon quantum dots
CN114225031A (en) * 2021-12-14 2022-03-25 贵州省烟草科学研究院 Tobacco-based carbon nanoenzyme and preparation method thereof
CN115058171A (en) * 2022-06-24 2022-09-16 中国铁道科学研究院集团有限公司金属及化学研究所 Low-surface-treatment water-based paint for station house steel structure and preparation method thereof
CN116942892A (en) * 2023-08-30 2023-10-27 南华大学附属第一医院 Hydrogel dressing for treating diabetes wound surface and preparation method thereof
CN116942892B (en) * 2023-08-30 2024-04-02 南华大学附属第一医院 Hydrogel dressing for treating diabetes wound surface and preparation method thereof

Similar Documents

Publication Publication Date Title
CN110669518A (en) Fluorescent carbon dot and preparation method and application thereof
Alonso et al. Determination of antioxidant power of red and white wines by a new electrochemical method and its correlation with polyphenolic content
CN109054826B (en) Red fluorescent carbon quantum dot and preparation method and application thereof
CN110562954B (en) Preparation method of fluorescent carbon dot probe and detection of Fe 2+ Application of
CN108358814B (en) AIE fluorescent probe based on salicylhydrazone derivative and preparation method and application thereof
CN110015992A (en) Fluorescence probe and its preparation method and application of the one kind for the detection of sulfur dioxide/sulfurous acid (hydrogen) salt
CN108956489A (en) A method of the class enzymatic property colorimetric detection hydrogen peroxide based on bimetallic Co/Mn-MOFs
CN113549449A (en) Nano fluorescent probe, preparation method and application thereof
Espinoza-Torres et al. Synthesis, characterization, and evaluation of a novel molecularly imprinted polymer (MIP) for selective quantification of curcumin in real food sample by UV-Vis spectrophotometry
CN105503768B (en) The preparation method of the fluorescence of alpha ketoglutaric acids/ultraviolet molecular probe and its application in biological specimen
CN109342341A (en) A method of utilizing carbon quantum dot Visual retrieval concentration of hydrogen peroxide
CN111662710B (en) Boron-nitrogen double-doped blue fluorescent carbon quantum dot and preparation method and application thereof
CN109394596A (en) A kind of preparation method and application for bringing back to life careless extract
CN105985291B (en) A kind of colorimetric fluorescence probe of quick high-selectivity analysis fluorine ion
CN105928912A (en) Heparin detection method
CN107383224A (en) The extraction of aloe polysaccharides in aloe and assay method
CN103091305A (en) Method for detecting quinolone antibiotic by means of electrochemical luminescence
CN103772528B (en) A kind of preparation method of selenium chitosan sulfuric ester and the application in animal and fowl fodder
CN112608744B (en) Green fluorescent carbon dot, preparation method thereof and application thereof in rutin detection
CN112326640A (en) Test paper for rapidly and directly reading and developing detection of lipid peroxide value, preparation method thereof, preparation method of standard colorimetric card and application thereof
CN103115882B (en) Detect H2O2Method (2)
CN113666843B (en) Large-Stokes-shift fluorescent probe for detecting viscosity and preparation and application thereof
CN107561070A (en) A kind of glucose quick visualization detection method and its preparation and application
CN101650335B (en) Preparation method of molecularly imprinted membrane detection device for tetracycline
CN102636526A (en) Electrochemical method for rapidly detecting oxidation resistance of bee product

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20200110