CN108235934A - A kind of method for changing tobacco external world growth conditions and promoting tobacco early blossoming - Google Patents
A kind of method for changing tobacco external world growth conditions and promoting tobacco early blossoming Download PDFInfo
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- CN108235934A CN108235934A CN201810362623.1A CN201810362623A CN108235934A CN 108235934 A CN108235934 A CN 108235934A CN 201810362623 A CN201810362623 A CN 201810362623A CN 108235934 A CN108235934 A CN 108235934A
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- tobacco
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
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Abstract
The present invention is a kind of method for changing tobacco external world growth conditions and promoting tobacco early blossoming.By the way that tobacco tissue-cultured seedling is taken out from culture bottle, transplanting is on Planting board after washing and sterilizing；Planting board is then positioned over plantation rooved face, sufficient nutrient solution is squeezed into advance in planting groove simultaneously, tobacco is planted by water planting, low temperature etc. is kept to change the technological means of tobacco external world growth conditions before buddingging, significantly promote tobacco early blossoming, shorten the tobacco growing time, disclosure satisfy that Functional identification of genes and the needs of tobacco breeding research, have a good application prospect.
Description
Technical field
The invention belongs to tobacco water planting technical fields, and in particular to a kind of change tobacco external world growth conditions promotes tobacco early
Colored method.
Background technology
Tobacco（Nicotiana tabacum）For Solanaceae（Solanaceae）Nicotiana（Nicotiana）It is annual or more
The Important Economic crop of year life, for plantation tobacco to obtain blade as the main purpose, yield of tobacco and interior quality influence its economy
Value.Southwest tobacco is mainly planted in the higher mountain area of height above sea level, in planting process, due to environmental factor, itself heredity
Easily there is early blossoming phenomenon in characteristic and field management.
Early blossoming phenomenon is both advantageous and disadvantageous, and for production, early flower fair causes tobacco plant not reach the kind or local cultivation
Due plant height and the number of blade just accelerate to complete growth and development dynamics and cross prematurity under the conditions of training, can seriously reduce the production of tobacco
Amount and quality, so as to reduce economic benefit.However for tobacco breeding worker, when early flower fair is obviously shortened tobacco breeding
Between.Main breed in China's production at present is relatively simple, only two big main breed of the big gold dollar of safflower and K326.It is badly in need of increasing
The progress of tobacco bred selection and breeding work.Tobacco breeding time is long, is a principal element for limiting tobacco breeding process.
Mo Chengen analyzes Guangxi Wuming early-flowering tobacco phenomenon, and current year tobacco seedlings are from the temperature during being transplanted to early blossoming
Situation is substantially at 18 DEG C or so.Chai Jiarong research shows that, tobacco is under the conditions of 18 DEG C or so, and no matter sunshine length can promote to spend
Bud breaks up, and the short time is under cryogenic conditions（Within one week）, much influences no on the differentiation of bud, if by 14 d, just
There is facilitation, up to the promotion bud differentiation of 21 d just significantly；Jiang Qinggen has then done the transplanting of 3 years from the angle of effective accumulated temperature
Phase experiment, large area investigation, carry out observation analysis, before finding crop field by the biological character to G-28 and breeding time accumulated temperature
To a certain extent, that is, when the effective accumulated temperature after transplanting in 30d is less than 200 DEG C, cigarette strain completes bud differentiation for the low temperature accumulation of phase,
Then budding soon and bloom.Cause early blossoming under the conditions of continuing 15 days less than 20 DEG C after the G28 variety transplantings of Guangdong Dapu County.
The present invention relates to a kind of methods for changing tobacco growing environment and promoting tobacco early blossoming, i.e., plant tobacco by water planting,
The means of low temperature are kept before buddingging, can significantly promote tobacco early blossoming, shortens the tobacco growing time, disclosure satisfy that Functional identification of genes
And the needs of tobacco breeding research, it has a good application prospect.
Invention content
The object of the present invention is to provide a kind of methods for changing tobacco external world growth conditions and promoting tobacco early blossoming, that is, pass through water
It cultivates and plants tobacco, the means of low temperature are kept before buddingging, can significantly promote tobacco early blossoming, shorten the tobacco growing period.
The method for changing tobacco external world growth conditions and promoting tobacco early blossoming of the present invention, includes the following steps：
Step（1）, tobacco tissue-cultured seedling is taken out from culture bottle, transplanting is on Planting board after washing and sterilizing；Then by Planting board
Plantation rooved face is positioned over, while squeezes into sufficient nutrient solution in planting groove in advance；Nutrient solution height about 3cm, so that tissue culture cigarette
The piece-root grafting of careless seedling contacts nutrient solution, and the temperature of the nutrient solution is 19 ± 1 DEG C of room temperature, and nutrient solution concentration is in tissue culture tobacco seedling
Phase keeps relatively low level, EC values（Conductivity）In 0.6mS/cm ± 0.05；
Step（2）, the culture of dark phase of 2 days by a definite date is carried out to tissue culture tobacco seedling, i.e., is directly shone without using LED light in 2 days
It penetrates, while to prevent tissue culture tobacco seedling moisture loss, is covered each tissue culture tobacco seedling 2 days using vial；
Step（3）, after the culture of dark phase of 2 days, vial is removed, the photo-irradiation treatment that routinizes is carried out to tobacco seedling, i.e., one
In its time, illumination 12h, the photo-irradiation treatment of dark 12h are carried out to tissue culture tobacco seedling, intensity of illumination is 300 μm of ol/ (m2
S), the ingredient of wherein light is mainly feux rouges and blue violet light；
Step（4）, treat that tobacco tissue culture growth of seedling after 15 days, turns down nutrient solution liquid level, is maintained at 1cm, and increase battalion
The concentration of nutrient solution makes EC values（Conductivity）0.65mS/cm ± 0.05 is maintained at, to meet tobacco growing needs；
Step（5）, before tobacco buddings, entire water planting indoor environment temperature is maintained at 17-21 DEG C of low temperature；After tobacco buddings, temperature
Degree is adjusted to tobacco growing normal value, and control day temperature is 28 DEG C, and nocturnal temperature is 25 DEG C；
Step（6）, in the overall process of tobacco cultivation, keep ambient humidity 60%-70%, a concentration of 400- of Carbon Dioxide in Air
500ppm。
Compared with prior art, the present invention advantage is as follows：
The present invention relates to tobacco water planting field, entire water planting indoor environment temperature, humidity, illumination are controllable, can basis
Tobacco different growing stage selects the condition of optimum tobacco growing, so as to which tobacco plant be made to grow up strong and sturdy, Rapid development, and root
The sound prosperity of system.
Tobacco is planted by water planting, squaring period keeps the means of low temperature to promote early blossoming, has easy to operate, effect
The advantages that good, easy to spread.
The present invention is using non-transgenic technology, different from turning early blossoming geneFTPromote the transgenic technology of early blossoming, no
It is safely controllable there are the risk of transgenosis drift.
Description of the drawings
Fig. 1 is tobacco solid water planting module schematic diagram.
Specific embodiment
With reference to embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and it should not be regarded as limiting this hair
Bright range.In the examples where no specific technique or condition is specified, according to the described technology of document in the art or condition
Or it is carried out according to product description.
Embodiment 1
The present invention needs to grow tobacco three-dimensional water planting module using one（As shown in Figure 1）, including water planting frame（In the present embodiment preferably
3 layers）, pipeline, nutrient solution tank.
If water planting module includes water planting frame 1,3 layers of planting groove 2 on water planting frame 1, two pieces of spellings on planting groove 2
The Planting board 3 connect and the nutrient solution tank connected with each 2 inlet 5 of layer planting groove and liquid outlet 6,2 surrounding of Planting board compare planting groove
Wide 1cm is covered on the opening of planting groove, and 12 implantation holes are set on every piece of Planting board, nutrient solution, cigarette are housed in planting groove 2
Careless seedling can be fixed in implantation hole 4 in a manner of existing, and the root of tobacco seedling is contacted across implantation hole 4 with nutrient solution, battalion
The pipeline connected on nutrient solution bucket with each layer planting groove inlet is equipped with pump, can nutrient solution be flowed into planting groove by inlet
In, nutrient solution is back to by liquid outlet in nutrient solution tank, forms the nutrient solution for circulating flowing.One layer of planting groove topmost
It as first layer, arranges down successively, every layer of height is 70cm, can meet tobacco growing.
Liquid outlet and inlet position can also set valve.When nutrient solution highly reaches requirement or is flowed,
Valve is opened, nutrient solution enters from inlet 5 or flowed out from liquid outlet 6, returns to nutrient solution tank.
The water planting on every layer of 2 top of planting groove is provided with several LED light 8 and mini-fan 7；Monitoring device is also provided with, is supervised
Control device is connect with thermometer, hygrometer and gas concentration lwevel sensor, detects temperature, the humidity and two of water planting module in real time
Carbonoxide concentration sensor.Tank gage can also be set to be connect with each layer planting groove 2, valve is electric control valve, monitoring device with
Electric control valve, tank gage connection, tank gage feedback information, the opening and closing of control valve are received by monitoring device.
Planting board is ecru PP materials, and thick 5mm, long 960mm, width 580mm, the surrounding of Planting board, which compares, plants groove width 1cm,
Increase the leakproofness of planting groove and Planting board.A length of 0.9 m of lamp of LED, power 18W, every layer each 24.
It is a kind of that tobacco early blossoming is promoted to obtain the method for short period tobacco, in the overall process of tobacco cultivation, keep environment
Humidity 60%-70%, a concentration of 400-500ppm of Carbon Dioxide in Air.
It mainly includes the following steps that
Step（1）, tobacco tissue culture seedling is taken out from culture bottle, transplanting is on Planting board after washing and sterilizing；It then will plantation
Plate is positioned over plantation rooved face, while squeezes into sufficient Huo Gelan in planting groove in advance（Hoagland）Nutrient solution（MgSO4·
7H2O, 30.81mg/L；Ca( NO3 )2·4H2O, 221.39mg/L；NaH2PO4·2H2O, 19.5mg/L；FeSO4·7H2O,
2.78mg/L；Na2- EDTA, 3.72mg/L；MnCl2·4H2O, 0.905mg/L；H3BO3, 1.43mg/L；ZnSO4·7H2O,
0.11mg/L；CuSO4·5 H2O, 0.04mg/L；( NH4 )6Mo7O24·2H2O, 0.045mg/L；K2SO4, 10.888mg/L.）
Nutrient solution height about 3cm, so that the piece-root grafting of tissue culture tobacco seedling contacts nutrient solution, the temperature of the nutrient solution is room temperature 19 ± 1
DEG C, nutrient solution concentration keeps relatively low level, EC values in the tissue culture tobacco seedling phase（Conductivity）In 0.6mS/cm ± 0.05.
Step（2）, the dark phase carried out to tobacco tissue culture seedling 2 days by a definite date cultivates, i.e., straight without using LED light progress in 2 days
Irradiation is connect, while to prevent tissue culture tobacco seedling moisture loss, is covered every plant of tissue culture tobacco seedling 2 days using vial.
Step（3）, after the culture of dark phase of 2 days, vial is removed, the photo-irradiation treatment that routinizes is carried out to tobacco seedling, i.e.,
In the time, illumination 12h, the photo-irradiation treatment of dark 12h are carried out to tissue culture tobacco seedling, intensity of illumination is 300 μm of ol/
(m2s), the ingredient of wherein light is mainly feux rouges and blue violet light.
Step（4）, treat that tobacco tissue culture growth of seedling after 15 days, turns down nutrient solution liquid level, is maintained at 1cm or so,
And the concentration of nutrient solution is increased, make EC values（Conductivity）0.65mS/cm ± 0.05 is maintained at, to meet tobacco growing needs.
Step（5）, before tobacco buddings, entire water planting indoor environment temperature is maintained at 17 DEG C of low temperature, after tobacco buddings, temperature
Degree is adjusted to tobacco growing normal value, and control day temperature is 28 DEG C, and nocturnal temperature is 25 DEG C.
Step（6）, flowering time is counted in the Tobacco Flowering phase, tobacco blooms for average 49 days.
Embodiment 2
The tobacco solid water planting module used is same as Example 1.
Step（1）To step（4）It is same as Example 1.
Step（5）, before tobacco buddings, entire water planting indoor environment temperature is maintained at 19 DEG C of low temperature.After tobacco buddings, temperature
Degree is adjusted to tobacco growing normal value, and control day temperature is 28 DEG C, and nocturnal temperature is 25 DEG C.
Step（6）, flowering time is counted in the Tobacco Flowering phase, tobacco blooms for average 41 days.
Embodiment 3
The tobacco solid water planting module used is same as Example 1.
Step（1）To step（4）It is same as Example 1.
Step（5）, before tobacco buddings, entire water planting indoor environment temperature is maintained at 19 DEG C of low temperature.After tobacco buddings, temperature
Degree is adjusted to tobacco growing normal value, and control day temperature is 28 DEG C, and nocturnal temperature is 25 DEG C.
Step（6）, flowering time is counted in the Tobacco Flowering phase, tobacco blooms for average 46 days.
It can be seen that by the comparison of upper table and short period tobacco obtained using a kind of promotion tobacco early blossoming of the present invention
Method, i.e., by water planting plant tobacco, before buddingging keep low temperature means, the Tobacco Flowering time can be reduced to 41-49 days,
It has been obviously shortened the tobacco growing period.
The basic principles, main features and the advantages of the invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (2)
- A kind of 1. method for changing tobacco external world growth conditions and promoting tobacco early blossoming, it is characterised in that：Include the following steps：Step（1）, tobacco tissue-cultured seedling is taken out from culture bottle, transplanting is on Planting board after washing and sterilizing；Then by Planting board Plantation rooved face is positioned over, while squeezes into sufficient nutrient solution in planting groove in advance；Nutrient solution height about 3cm, so that tissue culture cigarette The piece-root grafting of careless seedling contacts nutrient solution, and the temperature of the nutrient solution is 19 ± 1 DEG C of room temperature, and nutrient solution concentration is in tissue culture tobacco seedling Phase keeps relatively low level, and EC values are in 0.6mS/cm ± 0.05；Step（2）, the culture of dark phase of 2 days by a definite date is carried out to tissue culture tobacco seedling, i.e., is directly shone without using LED light in 2 days It penetrates, while to prevent tissue culture tobacco seedling moisture loss, is covered each tissue culture tobacco seedling 2 days using vial；Step（3）, after the culture of dark phase of 2 days, vial is removed, the photo-irradiation treatment that routinizes is carried out to tobacco seedling, i.e., one In its time, illumination 12h, the photo-irradiation treatment of dark 12h are carried out to tissue culture tobacco seedling, intensity of illumination is 300 μm of ol/ (m2 S), the ingredient of wherein light is mainly feux rouges and blue violet light；Step（4）, treat that tobacco tissue culture growth of seedling after 15 days, turns down nutrient solution liquid level, is maintained at 1cm, and increase battalion The concentration of nutrient solution makes EC values（Conductivity）0.65mS/cm ± 0.05 is maintained at, to meet tobacco growing needs；Step（5）, before tobacco buddings, entire water planting indoor environment temperature is maintained at 17-21 DEG C of low temperature；After tobacco buddings, temperature Degree is adjusted to tobacco growing normal value, and control day temperature is 28 DEG C, and nocturnal temperature is 25 DEG C；Step（6）, in the overall process of tobacco cultivation, keep ambient humidity 60%-70%, a concentration of 400- of Carbon Dioxide in Air 500ppm。
- 2. the method according to claim 1 for changing tobacco external world growth conditions and promoting tobacco early blossoming, it is characterised in that：Institute It is the Glan nutrient solution suddenly of tobacco dedicated 1/8 to state nutrient solution.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810362623.1A CN108235934B (en) | 2018-04-20 | 2018-04-20 | Method for changing external growth conditions of tobacco and promoting early flowering of tobacco |
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| CN201810362623.1A CN108235934B (en) | 2018-04-20 | 2018-04-20 | Method for changing external growth conditions of tobacco and promoting early flowering of tobacco |
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| CN108235934A true CN108235934A (en) | 2018-07-03 |
| CN108235934B CN108235934B (en) | 2020-04-21 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113416735A (en) * | 2021-03-17 | 2021-09-21 | 云南中烟工业有限责任公司 | Tobacco germ cell specific high expression gene and application thereof |
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| CN102960223A (en) * | 2012-11-14 | 2013-03-13 | 董爱文 | Method for simply, easily and quickly cultivating tobacco seedlings |
| CN103392574A (en) * | 2013-08-02 | 2013-11-20 | 中国烟草总公司重庆市公司烟草科学研究所 | Method for growing seedlings by using intermittent flow type nutrient liquid film |
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| CN106613827A (en) * | 2016-11-14 | 2017-05-10 | 福建省中科生物股份有限公司 | Roxburghanoectochilus factory cultivation method and cultivation module |
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2018
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| CN102960223A (en) * | 2012-11-14 | 2013-03-13 | 董爱文 | Method for simply, easily and quickly cultivating tobacco seedlings |
| CN103392574A (en) * | 2013-08-02 | 2013-11-20 | 中国烟草总公司重庆市公司烟草科学研究所 | Method for growing seedlings by using intermittent flow type nutrient liquid film |
| CN204949003U (en) * | 2015-06-18 | 2016-01-13 | 爱盛生物科技（上海）有限公司 | Three -dimensional plant soilless culture device |
| CN106613827A (en) * | 2016-11-14 | 2017-05-10 | 福建省中科生物股份有限公司 | Roxburghanoectochilus factory cultivation method and cultivation module |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113416735A (en) * | 2021-03-17 | 2021-09-21 | 云南中烟工业有限责任公司 | Tobacco germ cell specific high expression gene and application thereof |
| CN113416735B (en) * | 2021-03-17 | 2023-01-31 | 云南中烟工业有限责任公司 | Tobacco germ cell specific high expression gene and application thereof |
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| CN108235934B (en) | 2020-04-21 |
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