CN108226505A - Immune colloid gold detection card of sodium sulfocyanate and preparation method thereof - Google Patents
Immune colloid gold detection card of sodium sulfocyanate and preparation method thereof Download PDFInfo
- Publication number
- CN108226505A CN108226505A CN201611158307.XA CN201611158307A CN108226505A CN 108226505 A CN108226505 A CN 108226505A CN 201611158307 A CN201611158307 A CN 201611158307A CN 108226505 A CN108226505 A CN 108226505A
- Authority
- CN
- China
- Prior art keywords
- gold
- sodium sulfocyanate
- film
- coated
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Immune colloid gold detection card of sodium sulfocyanate of the present invention and preparation method thereof, is related to animal-derived food detection of veterinary drugs in food technical field.Test strips in the detection card shell of the present invention, are made of PVC offset plates, sample pad, gold conjugation pad, coated film and water absorption pad；Colloidal gold film is the glass fibre element film of the monoclonal antibody containing sodium sulfocyanate, and coated film is nitrocellulose filter, which is provided with T lines and C lines, and T lines are coated with sodium sulfocyanate protein conjugate, and C lines are coated with sheep anti-mouse igg antibody.The present invention is convenient, fast, result is accurate effective for quickly detecting sodium sulfocyanate.
Description
Technical field
The present invention relates to sodium sulfocyanate residue detection technical field in dairy products, more particularly to the immune glue of sodium sulfocyanate
Body gold detection card and preparation method thereof.
Background technology
Sodium sulfocyanate（NaSCN）It is naturally occurring a kind of active antibacterial system in lactogenesis, newborn peroxidating
Object enzyme system（It LPS) can be with bacteriostasis, preservation.1991, the CAC/GL 13- of Codex Committee on Food's announcement of WHO and FAO
1991《Lacto-peroxidase system is used for the fresh-keeping guide of raw milk》In, sodium sulfocyanate is allowed to add as the activator of LPS
It is added in milk；China also in subsequent issuing standard, allow to be added to it is fresh-keeping for raw milk in no cold chain transportation system,
Additive amount is 15mg/L.
But sodium sulfocyanate category poisonous and harmful substance, major toxicity act as inhibiting based on Activity of Cytochrome Oxidase
Acute toxicity with iodine to be inhibited to transport and the chronic toxicity that synthesize of thyroxine, especially to the brain of fetus and baby with it is neural
There are larger harm for systematic growth.With the food-safe attention degree raising of society and perfect, the country of former milk cold chain transportation
Standard before having abolished simultaneously is announced in December, 2008《The non-edible material from soybeans of possible illegal addition and the food easily abused in food
Product additive kind list（First）》, it is specified that sodium sulfocyanate belongs to illegal additive in milk and milk products.At present, China is still
The standard and limit value of sodium sulfocyanate in detection milk are not formulated.Ft%HWGE
The prior art is mainly the detection methods such as gas chromatography-mass spectrography, high performance liquid chromatography for the detection of sodium sulfocyanate,
But the defects of these technologies, is apparent：Equipment is expensive, it is complicated for operation, be difficult to promote.So establish a kind of simple, effective, suitable base
The assay method that layer uses is extremely necessary.It is more suitable for enterprise the purpose of the present invention is developing one kind and carries out Site Detection and fast
Prompt easy, low-cost qualitative checking method.
Invention content
For case above, the purpose of the present invention is exactly to provide a kind of sulphur cyanogen to overcome defect of the existing technology
Immune colloid gold detection card of sour sodium and preparation method thereof, can effectively solve quickly, easily detect asking for sodium sulfocyanate
Topic.
The sodium sulfocyanate colloidal-gold detecting-card of the present invention, the colloidal gold including being coated with monoclonal antibody colloid gold label object
Bonding pad, the nitrocellulose filter for being coated with sodium sulfocyanate-BSA and sheep anti-mouse igg, sample pad, water absorption pad, PVC offset plates and modeling
Expect mold composition, adhere to sample pad, bonding pad successively in one end of PVC offset plates, nitrocellulose filter is pasted in centre, and the other end glues
Attached water absorption pad.
The preparation method of the sodium sulfocyanate colloidal-gold detecting-card of the present invention, is realized by following steps：
(1) prepared by colloidal gold solution：1 L conical flasks 1 are taken, 495 mL of ultra-pure water is added in, then adds in 1% gold chloride
(HAuCl4·3H2O) 5 mL is configured to 500 mL, 0.01% aqueous solution of chloraurate, heats after boiling in the situation of lasting stirring
1% trisodium citrate (Na of lower addition3C6H5O7·2H2O) solution 5-7 mL continue agitating and heating, when the color of solution becomes completely
During transparent aubergine, stop heating after maintaining 5 min, moisturizing to original volume is cooled to room temperature, and 2-8 DEG C saves backup；
(2) pretreatment of antibody：The sodium sulfocyanate antibody that will be marked centrifuges 20 min under the conditions of 1000 r/min, 4 DEG C,
Supernatant is taken, 1 mg/mL is diluted to 0.01 mol/L PBS；Or 1 mg/ml is diluted to 0.01 mol/L PBS, cross 0.22
μm filter membrane；
(3) preparation of colloid gold label object：40 mL of colloidal gold solution in step (1) is taken, is adjusted with 0.25 mol/L K2CO3
Colloidal gold solution pH to 8.5,250 r/min of magnetic stirrer are stirred, and the egg that 4 mL contain 0.3 mg antibody proteins is added dropwise
White solution reacts 10 min；4 mL, 10% BSA are added dropwise, continue to be stirred to react 10 min；Gold labeling antibody solution room temperature is low
Speed（1500 r/min）10 min are centrifuged, discard the precipitation formed by the gold particle agglomerated；Red 4 DEG C of supernatant solution, 12000
R/min centrifuges 20 min, abandons supernatant, collects precipitation, precipitation is settled to 1 mL with gold labeling antibody dilution, is prepared into sulphur cyanogen
Sour sodium monoclonal antibody colloid gold label object；
(4) preparation of colloidal gold film：Step (3) sodium sulfocyanate protein conjugate monoclonal antibody colloid gold label object is used and is drawn
Film instrument is sprayed on the even concentration of 5 μ L/cm on carrier glass cellulose membrane, room temperature naturally dry or 37 DEG C of 3 h of drying, system
Into the colloidal gold film of the monoclonal antibody colloid gold label object of protein conjugate containing sodium sulfocyanate；
(5) it is coated with film preparation：Sheep anti-mouse igg antibody, sodium sulfocyanate protein conjugate are diluted to 1 mg/mL, with a stroke film instrument
It is sprayed on nitrocellulose filter with the concentration of 1 μ L/cm successively, is prepared into coated film, after 37 DEG C are coated with 2 h, room temperature is dried in the air naturally
Dry or 37 DEG C of drying；
(6) sample pad pre-treatment：Sample pad treatment fluid is uniformly coated on glass fibre element film, is less than 60% in air humidity
Under conditions of, room temperature naturally dry；
(7) assembling of detection card：PVC offset plates paste sample pad, colloidal gold film, coated film and absorbent wool, group successively from top to bottom
Test strips are dressed up, cut into the strip of one fixed width, then test strips are mounted in the flat shelly-shaped detection card shell of strip.
In the step (5) coated detection line T be located at the lower section of nature controlling line C；
Sample pad treatment fluid in the step (6) is by 1 g Bovine serum albumins (BSA) and 0.8 g sodium chloride (NaCl), is used
The 0.01 mol/L PBS containing 0.5%TRITON-100 are settled to 100 mL；
The present invention can be effectively used for measuring sodium sulfocyanate, and method is simple, convenient, fast, as a result accurately.
Description of the drawings
Fig. 1 is the structure chart of the immune colloid gold detection card of sodium sulfocyanate of the present invention：1. well, 2. detection line in figure
3. 4. detection hole of nature controlling line, 5. test strips 6. detect card shell.
Fig. 2 is the sectional structure chart of the test strips in the immune colloid gold detection of sodium sulfocyanate of the present invention blocks, 7. samples in figure
Product pad 8. 10. nitrocellulose filter of gold conjugation pad 9.PVC offset plates, 11. water absorption pad.
Specific embodiment
Embodiment 1
Fig. 1, embodiment shown in Fig. 2：9 be PVC offset plates in figure；7 be sample pad；8 be gold conjugation pad, which combines
Monoclonal antibody colloid gold label object has been coated on pad；10 be coated film, i.e. nitrocellulose filter, is wrapped on the nitrocellulose filter
By sodium sulfocyanate-BSA and sheep anti-mouse igg；11 be water absorption pad, is made of water-absorbent material such as filter paper.
(sample end) adherency sample pad 7, bonding pad 8, sample pad 7 and bonding pad 8 is side by side on one end of PVC offset plates 9
Structure.
In the intermediate adhesion nitrocellulose filter 10 of PVC offset plates 9.Sheep anti-mouse igg is provided on nitrocellulose filter 10
Nature controlling line 3 and sodium sulfocyanate-BSA detection lines 2.
Water absorption pad 11 is adhered in the other end of PVC offset plates 9.One end of nitrocellulose filter 10 slightly intersects with bonding pad 8, separately
One end slightly intersects with water absorption pad 11.The test strips 5 can be incorporated in detection card shell 6 made of plastic mould, and detection card is made,
Well 1 and detection hole 4 are equipped on the upper lid of detection card shell 6,7 face well 1 of sample pad, nitrocellulose filter 10 is just
To detection hole 4.
Embodiment 2
Prepared by sodium sulfocyanate colloidal-gold detecting-card, implemented by following steps：
It is prepared by colloidal gold solution：1 L conical flasks 1 are taken, 495 mL of ultra-pure water is added in, then adds in 1% gold chloride
(HAuCl4·3H2O) 5 mL is configured to 500 mL, 0.01% aqueous solution of chloraurate, heats after boiling in the situation of lasting stirring
1% trisodium citrate (Na of lower addition3C6H5O7·2H2O) solution 5-7 mL continue agitating and heating, when the color of solution becomes completely
During transparent aubergine, stop heating after maintaining 5 min, moisturizing to original volume is cooled to room temperature, and 2-8 DEG C saves backup；
The pretreatment of antibody：The sodium sulfocyanate antibody that will be marked centrifuges 20 min, takes under the conditions of 1000 r/min, 4 DEG C
Supernatant is diluted to 1 mg/mL with 0.01 mol/L PBS；Or 1 mg/ml is diluted to 0.01 mol/L PBS, cross 0.22 μ
M filter membranes；
The preparation of colloid gold label object：40 mL of colloidal gold solution in step (1) is taken, glue is adjusted with 0.25 mol/L K2CO3
Body gold solution pH to 8.5,250 r/min of magnetic stirrer are stirred, and the albumen that 4 mL contain 0.3 mg antibody proteins is added dropwise
Solution reacts 10 min；4 mL, 10% BSA are added dropwise, continue to be stirred to react 10 min；Gold labeling antibody solution room temperature low speed
（1500 r/min）10 min are centrifuged, discard the precipitation formed by the gold particle agglomerated；Red 4 DEG C of supernatant solution, 12000 r/
Min centrifuges 20 min, abandons supernatant, collects precipitation, precipitation is settled to 1 mL with gold labeling antibody dilution, is prepared into thiocyanic acid
Sodium monoclonal antibody colloid gold label object；
The preparation of colloidal gold film：By step (3) sodium sulfocyanate protein conjugate monoclonal antibody colloid gold label object stroke film
Instrument is sprayed on the even concentration of 5 μ L/cm on carrier glass cellulose membrane, and room temperature naturally dry or 37 DEG C of 3 h of drying are made
The colloidal gold film of the monoclonal antibody colloid gold label object of protein conjugate containing sodium sulfocyanate；
It is coated with film preparation：Sheep anti-mouse igg antibody, sodium sulfocyanate protein conjugate are diluted to 1 mg/mL, with stroke film instrument successively
Be sprayed on nitrocellulose filter with the concentration of 1 μ L/cm, be prepared into coated film, after 37 DEG C of 2 h of coating, room temperature naturally dry or
37 DEG C of drying of person；
Sample pad pre-treatment：Sample pad treatment fluid is uniformly coated on glass fibre element film, is less than 60% item in air humidity
Under part, room temperature naturally dry；
Detect the assembling of card：PVC offset plates paste sample pad, colloidal gold film, coated film and absorbent wool successively from top to bottom, are assembled into
Test strips cut into one fixed width strip, then test strips are mounted in the flat shelly-shaped detection card shell of strip.
Claims (2)
1. sodium sulfocyanate colloidal-gold detecting-card, it is characterised in that be prepared as steps described below：
(1) prepared by colloidal gold solution：1 L conical flasks 1 are taken, 495 mL of ultra-pure water is added in, then adds in 1% gold chloride
HAuCl4·3H2O) 5 mL is configured to 500 mL, 0.01% aqueous solution of chloraurate, heats after boiling in the case of lasting stirring
Add in 1% trisodium citrate Na3C6H5O7·2H2O solution 5-7 mL continue agitating and heating, when the color of solution becomes transparent completely
Aubergine when, stop heating after maintaining 5 min, moisturizing to original volume is cooled to room temperature, and 2-8 DEG C saves backup；
(2) pretreatment of antibody：The sodium sulfocyanate antibody that will be marked centrifuges 20 min under the conditions of 1000 r/min, 4 DEG C,
Supernatant is taken, 1 mg/mL is diluted to 0.01 mol/L PBS；Or 1 mg/ml is diluted to 0.01 mol/L PBS, cross 0.22
μm filter membrane；
(3) preparation of colloid gold label object：40 mL of colloidal gold solution in step (1) is taken, with 0.25 mol/L K2CO3It adjusts
Colloidal gold solution pH to 8.5,250 r/min of magnetic stirrer are stirred, and the egg that 4 mL contain 0.3 mg antibody proteins is added dropwise
White solution reacts 10 min；4 mL, 10% BSA are added dropwise, continue to be stirred to react 10 min；Gold labeling antibody solution room temperature
1500 r/min centrifuge 10 min, discard the precipitation formed by the gold particle agglomerated；Red 4 DEG C of supernatant solution, 12000 r/min
20 min are centrifuged, abandon supernatant, precipitation is collected, precipitation is settled to 1 mL with gold labeling antibody dilution, is prepared into sodium sulfocyanate list
Clonal antibody colloid gold label object；
(4) preparation of colloidal gold film：Step (3) sodium sulfocyanate protein conjugate monoclonal antibody colloid gold label object is used and is drawn
Film instrument is sprayed on the even concentration of 5 μ L/cm on carrier glass cellulose membrane, room temperature naturally dry or 37 DEG C of 3 h of drying, system
Into the colloidal gold film of the monoclonal antibody colloid gold label object of protein conjugate containing sodium sulfocyanate；
(5) it is coated with film preparation：Sheep anti-mouse igg antibody, sodium sulfocyanate protein conjugate are diluted to 1 mg/mL, with a stroke film instrument
It is sprayed on nitrocellulose filter with the concentration of 1 μ L/cm successively, is prepared into coated film, after 37 DEG C are coated with 2 h, room temperature is dried in the air naturally
Dry or 37 DEG C of drying；
(6) sample pad pre-treatment：Sample pad treatment fluid is uniformly coated on glass fibre element film, is less than 60% in air humidity
Under conditions of, room temperature naturally dry；
(7) assembling of detection card：PVC offset plates paste sample pad, colloidal gold film, coated film and absorbent wool, group successively from top to bottom
Test strips are dressed up, cut into the strip of one fixed width, then test strips are mounted in the flat shelly-shaped detection card shell of strip.
2. sodium sulfocyanate colloidal-gold detecting-card according to claim 1, it is characterised in that institute is coated in the step (5)
Detection line T is located at the lower section of nature controlling line C；
Sample pad treatment fluid in the step (6) is by 1 g Bovine serum albumins (BSA) and 0.8 g sodium chloride (NaCl), is used
The 0.01 mol/L PBS containing 0.5%TRITON-100 are settled to 100 mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611158307.XA CN108226505A (en) | 2016-12-15 | 2016-12-15 | Immune colloid gold detection card of sodium sulfocyanate and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611158307.XA CN108226505A (en) | 2016-12-15 | 2016-12-15 | Immune colloid gold detection card of sodium sulfocyanate and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108226505A true CN108226505A (en) | 2018-06-29 |
Family
ID=62650303
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611158307.XA Pending CN108226505A (en) | 2016-12-15 | 2016-12-15 | Immune colloid gold detection card of sodium sulfocyanate and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108226505A (en) |
Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1409113A (en) * | 2002-08-06 | 2003-04-09 | 张少恩 | Use of colloidal gold immunological chromatography in detection of aquatic, agricutural and livestock products |
| CN1746675A (en) * | 2004-09-07 | 2006-03-15 | 李人 | Immune chromatograph testing strip and production thereof |
| CN102901819A (en) * | 2012-09-27 | 2013-01-30 | 江苏维赛科技生物发展有限公司 | Immune colloidal gold detection card for phenylethanolamine A and preparation method thereof |
| CN102901818A (en) * | 2012-09-27 | 2013-01-30 | 江苏维赛科技生物发展有限公司 | Five-conjugate testing card for detecting beta-stimulant drugs and preparation method thereof |
| CN202854149U (en) * | 2012-08-16 | 2013-04-03 | 北京维德维康生物技术有限公司 | Gold-marking micropore kit for rapidly detecting small molecule compound |
| CN103018467A (en) * | 2012-09-27 | 2013-04-03 | 江苏维赛科技生物发展有限公司 | Colloidal gold immunochromatographic card for detecting bisphenol A (BPA) and preparation method thereof |
| CN103048455A (en) * | 2012-09-27 | 2013-04-17 | 江苏维赛科技生物发展有限公司 | Herbicide 2, 4-D and pesticide CHI bigeminy detection card and preparation method thereof |
| CN103808930A (en) * | 2012-11-06 | 2014-05-21 | 江苏维赛科技生物发展有限公司 | Zilpaterol immune colloidal gold detection card and preparation method thereof |
| CN104655835A (en) * | 2013-11-20 | 2015-05-27 | 南京亿特生物科技有限公司 | Immune colloidal gold detect card of chlorpromazine, and preparation method thereof |
| CN105277706A (en) * | 2014-07-25 | 2016-01-27 | 江苏维赛科技生物发展有限公司 | Cyproheptadine hydrochloride immune colloidal gold detection card and preparation method thereof |
| CN105510587A (en) * | 2014-10-16 | 2016-04-20 | 镇江先创生物科技有限公司 | Neomycin immuno-colloidal gold detection card and preparation method thereof |
| CN105572365A (en) * | 2014-10-13 | 2016-05-11 | 江苏维赛科技生物发展有限公司 | Immunocolloidal gold detection card for pesticide CHI and preparation method thereof |
| CN105572366A (en) * | 2014-10-13 | 2016-05-11 | 江苏维赛科技生物发展有限公司 | Immunocolloidal gold detection card for pretilachlor and preparation method thereof |
| CN105572360A (en) * | 2014-10-12 | 2016-05-11 | 江苏维赛科技生物发展有限公司 | Immune colloidal gold detection card of pretilachlor, and making method thereof |
| CN105572392A (en) * | 2014-10-16 | 2016-05-11 | 丹阳亿太生物科技发展有限公司 | Immunity colloid gold detecting card of herbicide glyphosate and preparing method of immunity colloid gold detecting card |
| CN105572390A (en) * | 2014-10-13 | 2016-05-11 | 江苏维赛科技生物发展有限公司 | Immunocolloidal gold detection card for herbicide 2,4-D and preparation method thereof |
| CN105572373A (en) * | 2014-10-17 | 2016-05-11 | 南京亿特生物科技有限公司 | Immune colloidal gold detecting card of carbendazin and preparation method of immune colloidal gold detecting card |
| CN105572372A (en) * | 2014-10-17 | 2016-05-11 | 南京亿特生物科技有限公司 | Immunity colloid gold detecting card of imidacloprid and preparing method of immunity colloid gold detecting card |
| CN105572371A (en) * | 2014-10-17 | 2016-05-11 | 南京亿特生物科技有限公司 | Immune colloidal gold detecting card of diclazuril and preparation method of immune colloidal gold detecting card |
-
2016
- 2016-12-15 CN CN201611158307.XA patent/CN108226505A/en active Pending
Patent Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1409113A (en) * | 2002-08-06 | 2003-04-09 | 张少恩 | Use of colloidal gold immunological chromatography in detection of aquatic, agricutural and livestock products |
| CN1746675A (en) * | 2004-09-07 | 2006-03-15 | 李人 | Immune chromatograph testing strip and production thereof |
| CN202854149U (en) * | 2012-08-16 | 2013-04-03 | 北京维德维康生物技术有限公司 | Gold-marking micropore kit for rapidly detecting small molecule compound |
| CN102901819A (en) * | 2012-09-27 | 2013-01-30 | 江苏维赛科技生物发展有限公司 | Immune colloidal gold detection card for phenylethanolamine A and preparation method thereof |
| CN102901818A (en) * | 2012-09-27 | 2013-01-30 | 江苏维赛科技生物发展有限公司 | Five-conjugate testing card for detecting beta-stimulant drugs and preparation method thereof |
| CN103018467A (en) * | 2012-09-27 | 2013-04-03 | 江苏维赛科技生物发展有限公司 | Colloidal gold immunochromatographic card for detecting bisphenol A (BPA) and preparation method thereof |
| CN103048455A (en) * | 2012-09-27 | 2013-04-17 | 江苏维赛科技生物发展有限公司 | Herbicide 2, 4-D and pesticide CHI bigeminy detection card and preparation method thereof |
| CN103808930A (en) * | 2012-11-06 | 2014-05-21 | 江苏维赛科技生物发展有限公司 | Zilpaterol immune colloidal gold detection card and preparation method thereof |
| CN104655835A (en) * | 2013-11-20 | 2015-05-27 | 南京亿特生物科技有限公司 | Immune colloidal gold detect card of chlorpromazine, and preparation method thereof |
| CN105277706A (en) * | 2014-07-25 | 2016-01-27 | 江苏维赛科技生物发展有限公司 | Cyproheptadine hydrochloride immune colloidal gold detection card and preparation method thereof |
| CN105572360A (en) * | 2014-10-12 | 2016-05-11 | 江苏维赛科技生物发展有限公司 | Immune colloidal gold detection card of pretilachlor, and making method thereof |
| CN105572365A (en) * | 2014-10-13 | 2016-05-11 | 江苏维赛科技生物发展有限公司 | Immunocolloidal gold detection card for pesticide CHI and preparation method thereof |
| CN105572366A (en) * | 2014-10-13 | 2016-05-11 | 江苏维赛科技生物发展有限公司 | Immunocolloidal gold detection card for pretilachlor and preparation method thereof |
| CN105572390A (en) * | 2014-10-13 | 2016-05-11 | 江苏维赛科技生物发展有限公司 | Immunocolloidal gold detection card for herbicide 2,4-D and preparation method thereof |
| CN105510587A (en) * | 2014-10-16 | 2016-04-20 | 镇江先创生物科技有限公司 | Neomycin immuno-colloidal gold detection card and preparation method thereof |
| CN105572392A (en) * | 2014-10-16 | 2016-05-11 | 丹阳亿太生物科技发展有限公司 | Immunity colloid gold detecting card of herbicide glyphosate and preparing method of immunity colloid gold detecting card |
| CN105572373A (en) * | 2014-10-17 | 2016-05-11 | 南京亿特生物科技有限公司 | Immune colloidal gold detecting card of carbendazin and preparation method of immune colloidal gold detecting card |
| CN105572372A (en) * | 2014-10-17 | 2016-05-11 | 南京亿特生物科技有限公司 | Immunity colloid gold detecting card of imidacloprid and preparing method of immunity colloid gold detecting card |
| CN105572371A (en) * | 2014-10-17 | 2016-05-11 | 南京亿特生物科技有限公司 | Immune colloidal gold detecting card of diclazuril and preparation method of immune colloidal gold detecting card |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105334319B (en) | A kind of test strips for detecting zearalenone in breast | |
| CN105572373A (en) | Immune colloidal gold detecting card of carbendazin and preparation method of immune colloidal gold detecting card | |
| CN107449908B (en) | A kind of preparation method and applications of colloid Buddha's warrior attendant carbon | |
| CN104655835A (en) | Immune colloidal gold detect card of chlorpromazine, and preparation method thereof | |
| CN112557654B (en) | Novel coronavirus antigen detection test strip | |
| CN105510587A (en) | Neomycin immuno-colloidal gold detection card and preparation method thereof | |
| CN109975544A (en) | A kind of PEDV IgY antibody colloidal gold test strip and its preparation method and application | |
| CN106248894A (en) | A kind of paper chip detecting Residue of Antibiotics in Milk | |
| CN112345753A (en) | Immunochromatography test strip prepared by taking polydopamine chrysanthemum-coated gold nanoparticles as beacon carriers | |
| CN105277706A (en) | Cyproheptadine hydrochloride immune colloidal gold detection card and preparation method thereof | |
| CN112034166A (en) | Kit for detecting total antibody of novel coronavirus in saliva and preparation method thereof | |
| CN107942052B (en) | A kind of colloidal gold kit and preparation method thereof detecting beta-stimulants | |
| CN101706499A (en) | FLAG fusion tag colloidal gold test strip and preparation method thereof | |
| CN106405088A (en) | Immunocolloidal-gold detection card for kitasamycin and preparation method thereof | |
| CN108226505A (en) | Immune colloid gold detection card of sodium sulfocyanate and preparation method thereof | |
| CN105572372A (en) | Immunity colloid gold detecting card of imidacloprid and preparing method of immunity colloid gold detecting card | |
| CN108226501A (en) | Immune colloid gold detection card of aflatoxin B1 and preparation method thereof | |
| CN109085353A (en) | A kind of Candida albicans colloidal-gold detecting-card and its application | |
| CN106568904A (en) | Immunity colloid gold detection card of paraquat and preparation method thereof | |
| CN108226502A (en) | Immune colloid gold detection card of Enrofloxacin and preparation method thereof | |
| CN106568946A (en) | immunogold detection card of propiconazole, and preparation method thereof | |
| CN103543274A (en) | Visual biological chip | |
| CN106568960A (en) | Immunocolloidal gold test card of methomyl and preparation method thereof | |
| CN114527289A (en) | Benzodiazepine BZO rapid detection kit | |
| CN106872690B (en) | A kind of high sensitivity colloid gold test paper and the preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180629 |
|
| WD01 | Invention patent application deemed withdrawn after publication |