CN105572371A - Immune colloidal gold detecting card of diclazuril and preparation method of immune colloidal gold detecting card - Google Patents
Immune colloidal gold detecting card of diclazuril and preparation method of immune colloidal gold detecting card Download PDFInfo
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- CN105572371A CN105572371A CN201410549891.6A CN201410549891A CN105572371A CN 105572371 A CN105572371 A CN 105572371A CN 201410549891 A CN201410549891 A CN 201410549891A CN 105572371 A CN105572371 A CN 105572371A
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- diclazuril
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Abstract
The invention discloses an immune colloidal gold detecting card of diclazuril and a preparation method of the immune colloidal gold detecting card, and relates to the technical field of animal source food veterinary drug residue detection. A test strip in a shell of the detecting card is composed of a PVC glue plate, a sample cushion, a colloidal gold conjugate pad, a coating film and a water absorbing cushion, wherein a colloidal gold film is a glass cellulose membrane containing diclazuril monoclonal antibodies, the coating film is a nitrocellulose film and is provided with a line T and a line C, the line T is wrapped by diclazuril protein conjugates, and the line C is wrapped by goat-anti-mouse IgG antibodies. The detecting card can be effectively used for rapidly detecting diclazuril and is convenient to use, rapid and accurate in result.
Description
Technical field
The present invention relates to cereal foods veterinary drug residue detection technique field, immune colloid gold test card particularly relating to diclazuril and preparation method thereof.
Background technology
Diclazuril (Diclazuril) belongs to triazine benzene acetonitrile compound, is off-white color or pale yellow powder, slightly molten in dimethyl formamide, and slightly soluble in tetrahydrofuran is almost insoluble in water, ethanol.Diclazuril is that a kind of wide spectrum that is efficient, low toxicity drives coccidia medicine, and have good prevention effect to various coccidias such as chicken, turkey, duck, goose, peacock, quail, rabbits, feed per ton adds 1g, effectively can control the outburst of global-worm illness.Eimeria species is the common parasitic worm of aviculture, causes serious financial consequences to poultry husbandry.The Main Means of current prevention chicken coccidiasis is that practical anticoccidial drug carries out chemical prevention and vaccine inoculation, and long-term agents prevention is the effective means for the treatment of and preventing coccidiosis of chicken always, and its dosage is large, accounts for 1/4 of various medicated premix total amount.Diclazuril is one of main coccidiostat used, and domestic practicality is also comparatively chaotic.
Along with the widespread use of medicine in food animal, its residue problem has caused and has paid close attention to widely.Medicament residue not only causes direct harm because of its toxic and side effect to people, and more serious problem is that the medicine of residual low concentration in food animal easily arrives again human disease bacterium generation drug resistance, thus is unfavorable for that medicine cures clinical application people.Because the residual research carrying out diclazuril is very necessary.
Prior art is for the detection mainly detection method such as high performance liquid chromatography of diclazuril, but the defect of these technology is obvious: apparatus expensive, complicated operation, be difficult to promote.So it is extremely necessary for setting up a kind of assay method that is simple, effective, that be applicable to basic unit's use.The object of the invention is a kind of enterprise that is more suitable for of development and carry out Site Detection and qualitative checking method fast and simple, with low cost.
Summary of the invention
For above situation, object of the present invention be exactly in order to overcome prior art exist defect and immune colloid gold test card that a kind of diclazuril is provided and preparation method thereof, effectively can solve the problem that can detect diclazuril fast, easily.
Diclazuril colloidal-gold detecting-card of the present invention, comprise and being formed by the nitrocellulose filter of diclazuril-BSA and sheep anti-mouse igg, sample pad, adsorptive pads, PVC offset plate and mould of plastics by the gold conjugation pad of monoclonal antibody colloid gold label thing, bag, sample pad, pad is adhered to successively in one end of PVC offset plate, nitrocellulose filter is pasted in centre, and the other end adheres to adsorptive pads.
The preparation method of diclazuril colloidal-gold detecting-card of the present invention is realized by following steps:
(1) colloidal gold solution preparation: get 1L Erlenmeyer flask 1, add ultrapure water 495mL, then add 1% gold chloride (HAuCl
43H
2o) 5mL, is mixed with 500mL0.01% aqueous solution of chloraurate, and heating adds 1% trisodium citrate (Na after boiling when Keep agitation
3c
6h
5o
72H
2o) solution 5-7mL, continues agitating heating, and when the color of solution becomes transparent aubergine completely, stop heating after maintaining 5min, moisturizing, to original volume, is cooled to room temperature, and 2-8 DEG C saves backup;
(2) pre-service of antibody: the diclazuril antibody that will mark is at 1000r/min, and under 4 DEG C of conditions, centrifugal 20min, gets supernatant, is diluted to 1mg/mL with 0.01mol/LPBS; Or be diluted to 1mg/ml with 0.01mol/LPBS, cross 0.22 μm of filter membrane;
(3) preparation of colloid gold label thing: get the colloidal gold solution 40mL in step (1), colloidal gold solution pH to 8.5 is regulated with 0.25mol/LK2CO3, magnetic stirrer 250r/min stirs, and dropwise adds the protein solution of 4mL containing 0.3mg antibody protein, reaction 10min; Dropwise add 4mL10%BSA, continue stirring reaction 10min; Gold labeling antibody solution normal temperature low speed (1500r/min) centrifugal 10min, discards the precipitation formed by the gold grain condensed; Red supernatant solution 4 DEG C, the centrifugal 20min of 12000r/min, abandons supernatant, collecting precipitation, and the golden labeling antibody dilution of precipitation is settled to 1mL, is prepared into diclazuril monoclonal antibody colloid gold label thing;
(4) preparation of colloidal gold film: step (3) diclazuril protein conjugate monoclonal antibody colloid gold label thing is sprayed on carrier glass cellulose membrane with drawing film instrument with the even concentration of 5 μ L/cm, room temperature is dried or 37 DEG C of oven dry 3h naturally, makes the colloidal gold film containing diclazuril protein conjugate monoclonal antibody colloid gold label thing;
(5) coated film preparation: sheep anti-mouse igg antibody, diclazuril protein conjugate are diluted to 1mg/mL, be sprayed on nitrocellulose filter with the concentration of 1 μ L/cm successively with drawing film instrument, be prepared into coated film, 37 DEG C of bags are by after 2h, and room temperature is dried or 37 DEG C of oven dry naturally;
(6) sample pad pre-treatment: be uniformly coated on by sample pad treating fluid on glass fibre element film, under the condition of air humidity lower than 60%, room temperature is dried naturally;
(7) assembling of test card: PVC offset plate pastes sample pad, colloidal gold film, coated film and absorbent wool from top to bottom successively, is assembled into test strips, cuts into the rectangular of one fixed width, then test strips is arranged in the test card shell of rectangular flat shelly.
In described step (5), the detection line T of institute's bag quilt is located at the below of nature controlling line C;
Sample pad treating fluid in described step (6) is by 1g Bovine serum albumin (BSA) and 0.8g sodium chloride (NaCl), is settled to 100mL with the 0.01mol/LPBS containing 0.5%TRITON-100;
The present invention can be effective to measure diclazuril, and method is simple, and convenient, fast, result is accurate.
Accompanying drawing explanation
Fig. 1 is the structural drawing of the immune colloid gold test card of diclazuril of the present invention: 1. well 2. detection line 3. nature controlling line 4. detect aperture 5. test strips 6. test card shells in figure.
Fig. 2 is the sectional structure chart of the test strips in the immune colloid gold test card of diclazuril of the present invention, 7. sample pad 8. gold conjugation pad 9.PVC offset plate 10. nitrocellulose filter 11. adsorptive pads in figure.
Embodiment
Embodiment 1
Embodiment shown in Fig. 1, Fig. 2: in figure, 9 is PVC offset plate; 7 is sample pad; 8 is gold conjugation pad, and on this gold conjugation pad, bag is by monoclonal antibody colloid gold label thing; 10 is coated film, i.e. nitrocellulose filter, and on this nitrocellulose filter, bag is by diclazuril-BSA and sheep anti-mouse igg; 11 is adsorptive pads, by absorbent material as filter paper is made.
On one end of PVC offset plate 9, (sample end) adheres to sample pad 7, pad 8, and sample pad 7 and pad 8 are side by side configuration.
At the intermediate adhesion nitrocellulose filter 10 of PVC offset plate 9.Nitrocellulose filter 10 is provided with sheep anti-mouse igg nature controlling line 3 and diclazuril-BSA detection line 2.
Adsorptive pads 11 is adhered at the other end of PVC offset plate 9.One end of nitrocellulose filter 10 slightly intersects with pad 8, and the other end slightly intersects with adsorptive pads 11.This test strips 5 can be incorporated with in the test card shell 6 that mould of plastics makes, and make test card, test card shell 6 covers and is provided with well 1 and detect aperture 4, sample pad 7 is just to well 1, and nitrocellulose filter 10 is just to detect aperture 4.
Embodiment 2
Prepared by diclazuril colloidal-gold detecting-card, be by following steps specific implementation:
Prepared by colloidal gold solution: get 1L Erlenmeyer flask 1, add ultrapure water 495mL, then add 1% gold chloride (HAuCl
43H
2o) 5mL, is mixed with 500mL0.01% aqueous solution of chloraurate, and heating adds 1% trisodium citrate (Na after boiling when Keep agitation
3c
6h
5o
72H
2o) solution 5-7mL, continues agitating heating, and when the color of solution becomes transparent aubergine completely, stop heating after maintaining 5min, moisturizing, to original volume, is cooled to room temperature, and 2-8 DEG C saves backup;
The pre-service of antibody: the diclazuril antibody that will mark is at 1000r/min, and under 4 DEG C of conditions, centrifugal 20min, gets supernatant, is diluted to 1mg/mL with 0.01mol/LPBS; Or be diluted to 1mg/ml with 0.01mol/LPBS, cross 0.22 μm of filter membrane;
The preparation of colloid gold label thing: get the colloidal gold solution 40mL in step (1), colloidal gold solution pH to 8.5 is regulated with 0.25mol/LK2CO3, magnetic stirrer 250r/min stirs, and dropwise adds the protein solution of 4mL containing 0.3mg antibody protein, reaction 10min; Dropwise add 4mL10%BSA, continue stirring reaction 10min; Gold labeling antibody solution normal temperature low speed (1500r/min) centrifugal 10min, discards the precipitation formed by the gold grain condensed; Red supernatant solution 4 DEG C, the centrifugal 20min of 12000r/min, abandons supernatant, collecting precipitation, and the golden labeling antibody dilution of precipitation is settled to 1mL, is prepared into diclazuril monoclonal antibody colloid gold label thing;
The preparation of colloidal gold film: step (3) diclazuril protein conjugate monoclonal antibody colloid gold label thing is sprayed on carrier glass cellulose membrane with drawing film instrument with the even concentration of 5 μ L/cm, room temperature is dried or 37 DEG C of oven dry 3h naturally, makes the colloidal gold film containing diclazuril protein conjugate monoclonal antibody colloid gold label thing;
Prepared by coated film: sheep anti-mouse igg antibody, diclazuril protein conjugate are diluted to 1mg/mL, be sprayed on nitrocellulose filter with the concentration of 1 μ L/cm successively with drawing film instrument, be prepared into coated film, 37 DEG C of bags are by after 2h, and room temperature is dried or 37 DEG C of oven dry naturally;
Sample pad pre-treatment: be uniformly coated on by sample pad treating fluid on glass fibre element film, under the condition of air humidity lower than 60%, room temperature is dried naturally;
The assembling of test card: PVC offset plate pastes sample pad, colloidal gold film, coated film and absorbent wool from top to bottom successively, is assembled into test strips, cuts into one fixed width rectangular, then test strips is arranged in the test card shell of rectangular flat shelly.
Embodiment 3
Diclazuril minimum detectability amount is tested
Precise 0.500g diclazuril antigen is dissolved in ultrapure water and is settled to 100mL, is designated as A
1solution, gets A
1solution 1mL adds ultrapure water in volumetric flask and is settled to 1000mL, forms A
2solution, gets A
2solution 1mL adds ultrapure water in volumetric flask and is settled to 1000mL, forms A
3solution, then the concentration of A3 solution is 5ng/mL (5ppb).Get A
3solution 1mL is in 4 test tubes, and difference label 1 ~ 4, add respectively in test tube toward 9mL, 4mL, 1mL, 0mL ultrapure water, be made into diclazuril antigen solution concentration respectively: 0.5ng/mL, 1ng/mL, 2.5ng/mL, 5ng/mL; Separately get an empty test tube, add 5mL ultrapure water, be designated as No. 5 test tubes.Getting liquid in above-mentioned 1 ~ No. 5 test tube is added in diclazuril test card as sample drop, and 5min observations after application of sample, the colour developing of detection line 2 and nature controlling line 3 the results are shown in Table 1.
Table 1 diclazuril minimum detectability amount is tested
Through repeatedly checking, the recall rate of diclazuril reaches 99%, and minimum detectability amount is 3ng/mL.
Claims (2)
1. diclazuril colloidal-gold detecting-card, is characterized in that preparing according to following step:
(1) colloidal gold solution preparation: get 1L Erlenmeyer flask 1, add ultrapure water 495mL, then add 1% gold chloride HAuCl
43H
2o) 5mL, is mixed with 500mL0.01% aqueous solution of chloraurate, and heating adds 1% trisodium citrate Na after boiling when Keep agitation
3c
6h
5o
72H
2o solution 5-7mL, continues agitating heating, and when the color of solution becomes transparent aubergine completely, stop heating after maintaining 5min, moisturizing, to original volume, is cooled to room temperature, and 2-8 DEG C saves backup;
(2) pre-service of antibody: the diclazuril antibody that will mark is at 1000r/min, and under 4 DEG C of conditions, centrifugal 20min, gets supernatant, is diluted to 1mg/mL with 0.01mol/LPBS; Or be diluted to 1mg/ml with 0.01mol/LPBS, cross 0.22 μm of filter membrane;
(3) preparation of colloid gold label thing: get the colloidal gold solution 40mL in step (1), use 0.25mol/LK
2cO
3regulate colloidal gold solution pH to 8.5, magnetic stirrer 250r/min stirs, and dropwise adds the protein solution of 4mL containing 0.3mg antibody protein, reaction 10min; Dropwise add 4mL10%BSA, continue stirring reaction 10min; The centrifugal 10min of gold labeling antibody solution normal temperature 1500r/min, discards the precipitation formed by the gold grain condensed; Red supernatant solution 4 DEG C, the centrifugal 20min of 12000r/min, abandons supernatant, collecting precipitation, and the golden labeling antibody dilution of precipitation is settled to 1mL, is prepared into diclazuril monoclonal antibody colloid gold label thing;
(4) preparation of colloidal gold film: step (3) diclazuril protein conjugate monoclonal antibody colloid gold label thing is sprayed on carrier glass cellulose membrane with drawing film instrument with the even concentration of 5 μ L/cm, room temperature is dried or 37 DEG C of oven dry 3h naturally, makes the colloidal gold film containing diclazuril protein conjugate monoclonal antibody colloid gold label thing;
(5) coated film preparation: sheep anti-mouse igg antibody, diclazuril protein conjugate are diluted to 1mg/mL, be sprayed on nitrocellulose filter with the concentration of 1 μ L/cm successively with drawing film instrument, be prepared into coated film, 37 DEG C of bags are by after 2h, and room temperature is dried or 37 DEG C of oven dry naturally;
(6) sample pad pre-treatment: be uniformly coated on by sample pad treating fluid on glass fibre element film, under the condition of air humidity lower than 60%, room temperature is dried naturally;
(7) assembling of test card: PVC offset plate pastes sample pad, colloidal gold film, coated film and absorbent wool from top to bottom successively, is assembled into test strips, cuts into the rectangular of one fixed width, then test strips is arranged in the test card shell of rectangular flat shelly.
2. diclazuril colloidal-gold detecting-card according to claim 1, is characterized in that the detection line T of institute's bag quilt in described step (5) is located at the below of nature controlling line C;
Sample pad treating fluid in described step (6) is by 1g Bovine serum albumin (BSA) and 0.8g sodium chloride (NaCl), is settled to 100mL with the 0.01mol/LPBS containing 0.5%TRITON-100.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410549891.6A CN105572371A (en) | 2014-10-17 | 2014-10-17 | Immune colloidal gold detecting card of diclazuril and preparation method of immune colloidal gold detecting card |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410549891.6A CN105572371A (en) | 2014-10-17 | 2014-10-17 | Immune colloidal gold detecting card of diclazuril and preparation method of immune colloidal gold detecting card |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108226500A (en) * | 2016-12-15 | 2018-06-29 | 江苏维赛科技生物发展有限公司 | The immune colloid gold detection blocking of fluoroquinolones Ciprofloxacin is standby |
| CN108226486A (en) * | 2016-12-15 | 2018-06-29 | 南京亿特生物科技有限公司 | Immune colloid gold detection card of Madumycin and preparation method thereof |
| CN108226502A (en) * | 2016-12-15 | 2018-06-29 | 江苏维赛科技生物发展有限公司 | Immune colloid gold detection card of Enrofloxacin and preparation method thereof |
| CN108226503A (en) * | 2016-12-15 | 2018-06-29 | 江苏维赛科技生物发展有限公司 | Immune colloid gold detection card of aflatoxin G 1 and preparation method thereof |
| CN108226501A (en) * | 2016-12-15 | 2018-06-29 | 江苏维赛科技生物发展有限公司 | Immune colloid gold detection card of aflatoxin B1 and preparation method thereof |
| CN108226505A (en) * | 2016-12-15 | 2018-06-29 | 南京亿特生物科技有限公司 | Immune colloid gold detection card of sodium sulfocyanate and preparation method thereof |
| CN109752544A (en) * | 2017-11-05 | 2019-05-14 | 江苏维赛科技生物发展有限公司 | A kind of colloidal gold strip detecting aflatoxin B1 |
| CN109813901A (en) * | 2017-11-21 | 2019-05-28 | 南京亿特生物科技有限公司 | A kind of immune colloid gold detection card and preparation method thereof detecting ethiprole |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1837771A (en) * | 2006-04-19 | 2006-09-27 | 姚家彪 | Analytic sample of animal drug residue in meat powder and preparation process thereof |
| CN102901819A (en) * | 2012-09-27 | 2013-01-30 | 江苏维赛科技生物发展有限公司 | Immune colloidal gold detection card for phenylethanolamine A and preparation method thereof |
| CN203037655U (en) * | 2012-07-27 | 2013-07-03 | 长沙安迪生物科技有限公司 | Rapid detection card for carbendazim |
-
2014
- 2014-10-17 CN CN201410549891.6A patent/CN105572371A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1837771A (en) * | 2006-04-19 | 2006-09-27 | 姚家彪 | Analytic sample of animal drug residue in meat powder and preparation process thereof |
| CN203037655U (en) * | 2012-07-27 | 2013-07-03 | 长沙安迪生物科技有限公司 | Rapid detection card for carbendazim |
| CN102901819A (en) * | 2012-09-27 | 2013-01-30 | 江苏维赛科技生物发展有限公司 | Immune colloidal gold detection card for phenylethanolamine A and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| YAHUI WANG ET AL.: "Development of a Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for the Analysis of Diclazuril in Chicken Tissues", 《FOOD ANALYTICAL METHOD》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108226500A (en) * | 2016-12-15 | 2018-06-29 | 江苏维赛科技生物发展有限公司 | The immune colloid gold detection blocking of fluoroquinolones Ciprofloxacin is standby |
| CN108226486A (en) * | 2016-12-15 | 2018-06-29 | 南京亿特生物科技有限公司 | Immune colloid gold detection card of Madumycin and preparation method thereof |
| CN108226502A (en) * | 2016-12-15 | 2018-06-29 | 江苏维赛科技生物发展有限公司 | Immune colloid gold detection card of Enrofloxacin and preparation method thereof |
| CN108226503A (en) * | 2016-12-15 | 2018-06-29 | 江苏维赛科技生物发展有限公司 | Immune colloid gold detection card of aflatoxin G 1 and preparation method thereof |
| CN108226501A (en) * | 2016-12-15 | 2018-06-29 | 江苏维赛科技生物发展有限公司 | Immune colloid gold detection card of aflatoxin B1 and preparation method thereof |
| CN108226505A (en) * | 2016-12-15 | 2018-06-29 | 南京亿特生物科技有限公司 | Immune colloid gold detection card of sodium sulfocyanate and preparation method thereof |
| CN109752544A (en) * | 2017-11-05 | 2019-05-14 | 江苏维赛科技生物发展有限公司 | A kind of colloidal gold strip detecting aflatoxin B1 |
| CN109813901A (en) * | 2017-11-21 | 2019-05-28 | 南京亿特生物科技有限公司 | A kind of immune colloid gold detection card and preparation method thereof detecting ethiprole |
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