CN108226040A - A kind of assay method and device of two-dimensional material photo-thermal effect - Google Patents
A kind of assay method and device of two-dimensional material photo-thermal effect Download PDFInfo
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- CN108226040A CN108226040A CN201810044280.4A CN201810044280A CN108226040A CN 108226040 A CN108226040 A CN 108226040A CN 201810044280 A CN201810044280 A CN 201810044280A CN 108226040 A CN108226040 A CN 108226040A
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- 239000000463 material Substances 0.000 title claims abstract description 52
- 230000000694 effects Effects 0.000 title claims abstract description 17
- 238000003556 assay Methods 0.000 title claims description 4
- 230000010287 polarization Effects 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 14
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 11
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229910021389 graphene Inorganic materials 0.000 claims abstract description 10
- 238000005259 measurement Methods 0.000 claims abstract description 10
- USWJSZNKYVUTIE-UHFFFAOYSA-N bis(sulfanylidene)rhenium Chemical compound S=[Re]=S USWJSZNKYVUTIE-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000010521 absorption reaction Methods 0.000 claims abstract 3
- CWQXQMHSOZUFJS-UHFFFAOYSA-N molybdenum disulfide Chemical compound S=[Mo]=S CWQXQMHSOZUFJS-UHFFFAOYSA-N 0.000 claims abstract 2
- 229910052982 molybdenum disulfide Inorganic materials 0.000 claims abstract 2
- 238000001514 detection method Methods 0.000 claims description 17
- 238000005086 pumping Methods 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 3
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims description 3
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims description 3
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 229910002804 graphite Inorganic materials 0.000 claims description 2
- 239000010439 graphite Substances 0.000 claims description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims 2
- 230000009102 absorption Effects 0.000 claims 2
- 239000012530 fluid Substances 0.000 claims 2
- 239000007787 solid Substances 0.000 claims 2
- 230000001419 dependent effect Effects 0.000 claims 1
- -1 graphite Alkene Chemical class 0.000 claims 1
- UGFMBZYKVQSQFX-UHFFFAOYSA-N para-methoxy-n-methylamphetamine Chemical compound CNC(C)CC1=CC=C(OC)C=C1 UGFMBZYKVQSQFX-UHFFFAOYSA-N 0.000 claims 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims 1
- 239000004926 polymethyl methacrylate Substances 0.000 claims 1
- 239000000377 silicon dioxide Substances 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 239000000523 sample Substances 0.000 abstract description 11
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000011160 research Methods 0.000 abstract description 4
- 238000011896 sensitive detection Methods 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 7
- 238000003384 imaging method Methods 0.000 description 5
- 239000010453 quartz Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 229910052582 BN Inorganic materials 0.000 description 2
- PZNSFCLAULLKQX-UHFFFAOYSA-N Boron nitride Chemical compound N#B PZNSFCLAULLKQX-UHFFFAOYSA-N 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 229910052976 metal sulfide Inorganic materials 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 238000000985 reflectance spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating Or Analyzing Materials Using Thermal Means (AREA)
Abstract
A kind of method of highly sensitive detection two-dimensional material photo-thermal effect, polarization absorption effect based on pump probe technology and two-dimensional material under total internal reflection structure, two-dimensional material is irradiated using pump light, two-dimensional material generates heat after absorbing light, and be transmitted in surrounding medium, since temperature change causes surrounding medium refraction index changing during heat propagation.It will detect light using convex lens and focus on and irradiated in two-dimensional material by prism total internal reflection mode, reflected light is separated the light of s polarizations and p-polarization using polarization spectro, the electric signal difference of s polarizations and p-polarization is measured by balanced detector, and then obtains the variations in refractive index caused by photo-thermal effect.Pump light is modulated in the measurements, and modulation intelligence is fed back in lock-in amplifier, while differential detector electric signal is input to lock-in amplifier, measurement to Photothermal Signals is realized using phase enlarging function is locked.The present invention is suitable for the photo-thermal effect of the different two-dimensional materials such as research graphene, molybdenum disulfide, black phosphorus, rhenium disulfide.
Description
Technical field
The invention belongs to two-dimensional materials and Opto-thertnal detection technical field, are related to a kind of using pump probe technology and complete interior anti-
Penetrate two-dimensional material photo-thermal effect assay method and device that structure is combined.
Background technology
After photo-thermal effect refers to the irradiation of material light, photon energy interacts with lattice, vibration aggravation, temperature raising, by
The electrology characteristic of substance is caused, and as heat propagation causes the variation of surrounding medium refractive index in the variation of temperature.Utilize light
The detector of fuel factor：Thermistor, thermocouple, thermoelectric pile and pyroelectric detector etc..Photo-thermal effect is also used as a kind of optics
The Measurement for Biotechnique of dependence has the advantages such as high-resolution, high contrast, can realize photothermal imaging, with other bio-imagings
Good complementation is formd with detection method.Pump light research object is absorbed in photo-thermal effect, followed by releases heat
Cause the variation of local indexes of refraction.Photothermal imaging technology provides very sensitive hand to measure the non-fluorescent molecules in solution
Section.Research gold nano grain is already used to, directly intracellular mitochondria is imaged or even is realized sensitive unimolecule examines
It surveys.At present, photothermal imaging is mainly the scattering of detection light to be caused to measure using thermotropic variations in refractive index, it is desirable that photo-thermal into
As experimental provision is often extremely complex.
The two-dimensional materials such as graphene, transient metal sulfide (TDMCs), hexagonal boron nitride (hBN), black phosphorus are due to its uniqueness
Two-dimensional structure and excellent photoelectron performance and obtain extensive research.In these two-dimensional materials, graphene, curing
Molybdenum, hexagonal boron nitride etc. have isotropic electricity, optics, calorifics and mechanical property in plane, the tools such as black phosphorus, rhenium disulfide
There are electricity, optics, calorifics and the mechanical property of anisotropic.For the photo-thermal effect of these two-dimensional materials, bio-imaging,
Biological detection etc. has potential application value, however how to measure the photo-thermal effect of these two-dimensional materials, does not have also at present
There is ripe method, especially to the monocrystal thin films of high quality, due to the limitation of its dimensional structure, traditional Opto-thertnal detection technology
It can not realize the photothermal measurement of two-dimensional material.
Invention content
The object of the present invention is to provide a kind of methods and dress of measurement two-dimensional material photo-thermal effect that can be highly sensitive
It puts, so as to the more accurately photo-thermal property of study two-dimensional material and its application in bio-sensing etc..
To achieve the above object, the step of the method for the invention is as follows：
The first step, the preparation of two-dimensional material are made on prism on a quartz substrate or directly with the method for mechanical stripping
It is standby, obtain the sample of mechanical stripping；
Second step does a sink around two-dimensional material with PDMS, has added liquid medium and has used coverslip again later
Encapsulation, in order to avoid generate bubble；
Third walks, and detection light part is incident using circularly polarized light or non-polarized light, is converged to by 50 times of object lens with mesh
The prism of two-dimensional material and the interface of base material are marked, total reflection light is divided into the two of s polarizations and p-polarization using polarization spectro
Shu Guang uses balanced detector detection s polarizations and the electric signal of p-polarization after being focused on respectively with object lens mirror.Adjust height and prism
Angle makes focal beam spot beat on the base material contacted with graphene, and is totally reflected at the interface, is moved with translation stage
Dynamic base material overlaps two-dimensional material and detection hot spot；
After 4th step, two-dimensional material sample and detection hot spot overlap, the attenuator before adjustment detector makes s inclined
It shakes as the intensity of p-polarization light；
5th step introduces the linear polarization pump light by acousto-optic modulator modulation, and the pump light that will have been focused on, detection light is also
There is sample three coincidence；
Acousto-optic modulator feeds back to the modulating frequency of pump light lock-in amplifier, while will detect flash ranging by the 6th step
The s polarizations of amount and the electric signal of p-polarization are input to lock-in amplifier and carry out signal measurement, by the angle of polarization for changing pump light
Degree, to record the variation of Photothermal Signals.
Description of the drawings
The light path schematic diagram of Fig. 1 Photothermal Signals detection；
The optics picture and Photothermal Signals of Fig. 2 graphene samples are by force with the datagram that pump light polarization angle changes；
The optics picture and Photothermal Signals of Fig. 3 black phosphorus samples are by force with the datagram that pump light polarization angle changes；
The optics picture and Photothermal Signals of Fig. 4 rhenium disulfide samples are by force with the data that pump light polarization angle changes
Figure.
Specific embodiment
In order to further illustrate the present invention, below in a manner of attached drawing and with reference to example to two-dimensional material provided by the invention
Photo-thermal effect measuring method and device are described in detail, but cannot be understood as limiting the scope of the present invention.With
Material and instrument employed in lower embodiment are commercially available.In addition, any method similar or impartial to described content and
Material is all applied in the method for the present invention.
Embodiment 1
The detection of graphene Photothermal Signals
As shown in Figure 2 a, few layer graphene is prepared on a quartz substrate with the method for mechanical stripping first, in few layer of graphite
PDMS sinks are made around alkene, alcohol is added dropwise as photo-thermal medium, is finally encapsulated with coverslip.The pump light that will have been focused on is visited
It surveys light and sample overlaps and generates Photothermal Signals later.Graphene Photothermal Signals are observed by changing the polarization angle of pump light
Variation.Because of isotropism in graphene face, significant change does not occur for the size of Photothermal Signals in figure 2b.
Embodiment 2
Fig. 3 a are shown prepares few layer of black phosphorus sample on a quartz substrate with the method for mechanical stripping.Black phosphorus is in face
Anisotropic material.With the thickness of atomic force microscope (AFM) test sample, obtaining the thickness of different zones sample is respectively
5nm, 8nm and 13nm, the Photothermal Signals of test different zones black phosphorus sample have obtained Fig. 3 b.Wherein Photothermal Signals maximum when pair
Black phosphorus crystal orientation AC directions are answered, minimum value corresponds to black phosphorus ZZ directions, and by testing the reflectance spectrum of black phosphorus, the above results, which are obtained for, to be tested
Card.The pumping light power 300 μ W, detection of optical power 3mW used in test.
Embodiment 3
Fig. 4 a show rhenium disulfide micro-image, and rhenium disulfide equally has intra-face anisotropy, with AFM test samples
Thickness.And in different-thickness region, the Photothermal Signals of the polarization angle test sample by changing pump light.Obtained data
Figure such as Fig. 4 b.The pumping light power 300 μ W, detection of optical power 5mW used in test.
Claims (9)
1. a kind of assay method of two-dimensional material photo-thermal effect, it is characterised in that the light of various two-dimensional materials can be measured in real time
Thermal signal, the method include：Two-dimensional material is attached to prism surface first, and one layer is covered thoroughly above two-dimensional material
Bright medium will be irradiated to by medium in two-dimensional material by the pump light that acousto-optic modulator is modulated, and two-dimensional material absorbs pumping
Heat is generated after light, and is transmitted in the medium around covered, medium causes the change of refractive index due to temperature change.Using convex
Lens, which will detect light and focus on, to be irradiated by prism total internal reflection mode in two-dimensional material, and reflected light is polarized s using polarization spectro
It is separated with the light of p-polarization, the electric signal difference of s polarizations and p-polarization is measured by balanced detector, and then obtain imitating due to photo-thermal
It should caused variations in refractive index.Pump light is modulated in the measurements, and modulation intelligence is fed back in lock-in amplifier, together
When differential detector electric signal is input to lock-in amplifier, realize measurement to Photothermal Signals using phase enlarging function is locked.
2. according to the method described in claim 1, it is characterized in that, measured two-dimensional material includes：Isotropic two dimension
Material, such as graphene, molybdenum disulfide；Anisotropic two-dimensional material, such as black phosphorus, rhenium disulfide.
3. according to the method described in claim 1, it is characterized in that, the combination of two-dimensional material and prism can be directly by graphite
Alkene, which attaches to, to be attached on prism or first by two-dimensional material on transparent sheet, is then mutually tied with prism by index-matching fluid
It closes.
4. according to the method described in claim 1, it is characterized in that, the medium covered above two-dimensional material includes：Solid dielectric,
Such as PMMA, PDMS, silica；Liquid medium, such as water, alcohol, chloroform.
5. a kind of measurement device of two-dimensional material photo-thermal effect, which is characterized in that including：Light unit is detected, while there is S-polarization
It can be circularly polarized light, 45 degree of linearly polarized lights or non-polarized light with two kinds of polarised lights of P polarization, emergent light is divided into s polarizations and p
Two-beam is polarized, measures the opposite variation of two kinds of polarised lights；Light unit is pumped, certain frequency is modulated into using modulator,
For the absorption of two-dimensional material；Prism total reflection structural unit with two-dimensional material, dependent on Jie contacted with two-dimensional material
Matter generates different absorptions with variations in refractive index caused by temperature to s polarizations and p-polarization；；Phase amplifying unit is locked, using locking phase
Amplifier obtains the detectable signal with pumping light modulation frequency, realizes the measurement of photo-thermal effect.
6. device according to claim 5, which is characterized in that detection light unit includes：With s polarizations and p-polarization component
Detection light；Detection light, which will be reflected, using polarization spectro is divided into s polarizations and p-polarization light；Two kinds are polarized using balanced detector
Light measure simultaneously, obtain as the electric signal caused by polarization variations.
7. device according to claim 5, which is characterized in that pumping light unit includes：Pump light with linear polarization；It can
The acousto-optic modulator or electrooptic modulator of intensity modulation are realized to pump light；For control the polarizer of pump light polarization variations and
Wave plate.
8. device according to claim 5, which is characterized in that the prism total reflection structural unit packet with two-dimensional material
It includes：The combination of two-dimensional material and prism can directly attach to two-dimensional material to attach on prism or first by two-dimensional material
On transparent sheet, then it is combined by index-matching fluid and prism；Solid dielectric or liquid medium are covered in two-dimentional material
Expect surface.
9. device according to claim 5, which is characterized in that lock phase amplifying unit includes：Lock phase enlarging function can be achieved
Lock-in amplifier；Pumping light modulation frequency is fed back into lock-in amplifier, using lock-in amplifier to the spy comprising the frequency
Light signal measures.
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Cited By (5)
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| CN109238968A (en) * | 2018-10-23 | 2019-01-18 | 哈尔滨工业大学 | A kind of photo-thermal heterodyne micro-imaging detection system and method |
| CN109342325A (en) * | 2018-10-30 | 2019-02-15 | 南开大学 | A kind of imaging method and device that low-dimensional materials anisotropy is micro- |
| CN112763421A (en) * | 2021-01-18 | 2021-05-07 | 太原理工大学 | Graphene GH displacement and photothermal effect-based solution detection device and method |
| CN112763726A (en) * | 2021-01-18 | 2021-05-07 | 太原理工大学 | Graphene photothermal sensing-based lung cancer marker miRNA quantitative detection method |
| CN114384018A (en) * | 2021-12-27 | 2022-04-22 | 中国科学院福建物质结构研究所 | Measuring device for measuring weak anisotropy in large-size isotropic transparent medium, measuring method and application |
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| CN114384018A (en) * | 2021-12-27 | 2022-04-22 | 中国科学院福建物质结构研究所 | Measuring device for measuring weak anisotropy in large-size isotropic transparent medium, measuring method and application |
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