CN108220449A - A kind of method and its special primer pair that detection pig is assisted to reach 100kg weight ages in days - Google Patents
A kind of method and its special primer pair that detection pig is assisted to reach 100kg weight ages in days Download PDFInfo
- Publication number
- CN108220449A CN108220449A CN201611138579.3A CN201611138579A CN108220449A CN 108220449 A CN108220449 A CN 108220449A CN 201611138579 A CN201611138579 A CN 201611138579A CN 108220449 A CN108220449 A CN 108220449A
- Authority
- CN
- China
- Prior art keywords
- pig
- genotype
- days
- primer pair
- ssc7g
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of methods and its special primer pair identified pig and reach 100kg weight ages in days.The present invention discloses a kind of primer pair for expanding the DNA fragmentation containing SSC7g.34981027 C ＞ T polymorphic sites；The SSC7g.34981027 C ＞ T polymorphic sites are No. 7 the 34981027th bit bases of chromosome of pig genome (Sscrofa10.2 Primary Assembly).Using method disclosed by the invention to pig carry out breeding, can to pig to be selected carry out early stage selection and breeding, effectively alleviate actual production in the selection excellent boar time it is long the problem of, reduce breeding cost, can effectively do sth. in advance pig reach 100kg weight ages in days.Method disclosed by the invention is easy to operate, expense is low, accuracy is high, and can realize the direct detection of automation, will play a great role in the breeding work of pig.
Description
Technical field
The present invention relates to a kind of methods and its special primer pair that detection pig is assisted to reach 100kg weight ages in days, belong to biology
Technical field.
Background technology
The speed of growth of pig is always the important component of pig breeding target, and Breeding value is very high.But due to life
Long speed index only grows to 6 months or so in pig up to 100kg weight age in days and can just measure, on the one hand increase breeding into
This, has on the other hand elongated the generation inteval, genetic progress is slow.The rapid development of molecular biotechnology and pig genetic linkage maps
Structure, for early detection pig up to 100kg weight ages in days character carry out breeding provide Research foundation.
Invention content
The object of the present invention is to provide a kind of methods and its special primer pair that detection pig is assisted to reach 100kg weight ages in days.
The present invention provides a kind of primer pair for expanding the DNA fragmentation containing SSC7g.34981027C ＞ T polymorphic sites；
The SSC7g.34981027C ＞ T polymorphic sites are with reference to pig genome (Sscrofa10.2 Primary
Assembly) No. 7 chromosome the 34981027th；
In above-mentioned primer pair, the primer pair is as shown in the DNA molecular shown in SEQ ID No.1 and SEQ ID No.2
DNA molecular forms.
A kind of kit for assisting detection pig up to 100kg weight ages in days also belongs to protection scope of the present invention, the kit
Include any of the above-described primer pair；
For the kit also comprising operation instructions, content described in specification is as follows：Using the genomic DNA of pig to be measured as
Template carries out PCR amplification by primer of any of the above-described primer pair, obtains pcr amplification product, which contains
Have with reference to No. 7 the 34981027th bit bases of chromosome of pig genome (Sscrofa10.2 Primary Assembly), if should
Locate base be C, then the genotype of the pig be homozygosis CC genotype, if at this base be C and T, the gene of the pig
Type is heterozygosis CT genotype, if base is T at this, the genotype of the pig is homozygosis TT genotype；Homozygous CC genes
The age in days of the pig of type up to the age in days of 100kg weight or pig up to the 100kg weight of heterozygosis CT genotype is all higher than homozygous TT genes
The pig of type reaches the age in days of 100kg weight.
In mentioned reagent box, the pig is duroc.
A kind of method for assisting detection pig up to 100kg weight ages in days also belongs to protection scope of the present invention, and this method is as follows：
The age in days of the pig of homozygous CC genotype up to the age in days of 100kg weight or pig up to the 100kg weight of heterozygosis CT genotype is all higher than
The pig of homozygous TT genotype reaches the age in days of 100kg weight.
The pig of the homozygosis CC genotype is that the base of SSC7g.34981027C ＞ T polymorphic sites is the pig of C；
It is C and the pig of T that the pig of the heterozygosis CT genotype, which is the base of SSC7g.34981027C ＞ T polymorphic sites,；
The pig of the homozygosis TT genotype is that the base of SSC7g.34981027C ＞ T polymorphic sites is the pig of T；
The SSC7g.34981027C ＞ T polymorphic sites are pig genome (Sscrofa10.2 Primary
Assembly) No. 7 chromosome the 34981027th；
In the above method, the determination method of the homozygosis CC genotype, heterozygosis CT genotype or homozygosis TT genotype is as follows：
Using the genomic DNA of pig as template, PCR amplification is carried out by primer of any of the above-described primer pair, obtains PCR amplification production
Object, if No. 7 chromosomes of pig genome (Sscrofa10.2 Primary Assembly) contained in pcr amplification product
34981027 bit bases are C, then the genotype of the pig is homozygosis CC genotype, described if base is C and T at this
The genotype of pig is heterozygosis CT genotype, if base is T at this, the genotype of the pig is homozygosis TT genotype.
In any of the above-described method, when the primer is above-mentioned primer pair, the pig genome (Sscrofa10.2
Primary Assembly) No. 7 the 34981027th bit bases of chromosome be located at the PCR amplified productions from 5 ' ends
169.
In any of the above-described method, the pig is duroc.
Any of the above-described primer pair, mentioned reagent box and/or any of the above-described method are in the selection and breeding of pig
Using also belonging to protection scope of the present invention.
In above application, the pig is duroc.
Method provided by the invention is by detecting pig genome (Sscrofa10.2 Primary Assembly) 7
The 34981027th bit base of chromosome differentiates the genotype of pig individual, further reaches 100Kg weight ages in days to pig by genotype
Character selected, can make pig up to 100kg weight age in days shorten about 12.06 days, so as to obtain the higher pig of production performance.
Breeding is carried out to pig using method provided by the invention, early screening can be carried out to pig to be selected, effectively alleviated real
Border production in choose the excellent boar time it is long the problem of, reduce breeding cost, can effectively shift to an earlier date actual production in pig reach
The age in days of 100kg weight.Method provided by the invention is easy to operate, expense is low, accuracy is high, and can realize the direct of automation
Detection, will play a great role in the breeding work of pig.
Description of the drawings
Fig. 1 is the sequencing result figure of SSC7g.34981027C ＞ T location proximate sequences.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Duroc (Sus scrofa), the pig are purchased from Institute of Animal Sciences, Chinese Academy of Agricultural Sciences Changping livestock and poultry
Experimental Base.
PCR amplification sequence in following embodiments is each meant with reference to pig genome (Sscrofa10.2 Primary
Assembly) sequence.
The method that embodiment 1, identification pig reach 100kg ages in days
First, SSC7g.34981027C ＞ T polymorphic sites is determining
(1) using both ends duroc as experiment material, the genomic DNA of its ear-edge tissue is extracted respectively.
(2) design and synthesis of primer
According to international 10.2 version reference sequences (http of pig genome://asia.ensembl.org/Sus_scrofa/
Info/Index), following primer is designed and synthesized：
U (sense primer)：5′-TTGTCTCCCAAACACCAT-3′(SEQ ID No.1)；
D (downstream primer)：5′-AGATACGCGGCTCCTAG-3′(SEQ ID No.2).
(3) PCR amplification
The genomic DNA of the duroc obtained respectively using step (1) carries out PCR expansions as template, by primer of U and D
Increase, obtain pcr amplification product, be respectively designated as product 1 and product 2.
PCR amplification system：Genomic DNA 200ng, 10 × PCR amplification buffer solution, the 5 μ final concentration of 10mM of l, dNTPs,
Each 50ng of upstream and downstream primer, Taq archaeal dna polymerases 0.75U, Mg2+2.5mmol/L uses ddH2O supplies system to 50 μ l.
PCR amplification program：95 DEG C of pre-degeneration 5min；95 DEG C of denaturation 20s, 60.5 DEG C of annealing 30s, 72 DEG C of extension 30s, totally 35
A cycle；Last 72 DEG C of extensions 10min.
(4) sequencing and sequence analysis
Product 1 and product 2 are sequenced, obtain the sequence (as shown in SEQ ID No.3) of product 1 and the sequence of product 2
Row are (as shown in SEQ ID No.4).SEQ ID No.3 and SEQ ID No.4 only exist the difference of a base, are SEQ ID
In No.3 and SEQ ID No.4 the 169th from 5 ' ends.Base at this is C or T, as shown by the arrows in Figure 1, the site
For No. 7 chromosomes the 34981027th of pig genome (Sscrofa10.2 Primary Assembly), therefore the site is ordered
Entitled SSC7g.34981027C ＞ T.
In the base of pig genome (Sscrofa10.2 Primary Assembly) No. 7 chromosome the 34981027th
(or the obtained pcr amplification product of step (3) the 169th base from 5 ' ends) is the individual of C, which is homozygosis
Type individual, is named as homozygous CC genotype, in pig genome (Sscrofa10.2 Primary by the genotype of the individual
Assembly) (or the pcr amplification product that step (3) obtains is from 5 ' ends for the base of No. 7 chromosome the 34981027th
The base of 169) be T individual, the individual be homozygous individual, the genotype of the individual is named as homozygous TT genes
Type, pig genome (Sscrofa10.2 Primary Assembly) No. 7 chromosome the 34981027th base (or step
Suddenly the pcr amplification product that (three) obtain base of the 169th from 5 ' ends) be C and T individual, the individual is a for heterozygous
The genotype of the individual is named as heterozygosis CT genotype by body.
2nd, SSC7g.34981027C ＞ T polymorphic sites reach the correlation analysis of 100kg ages in days with pig
To determine whether SSC7g.34981027C ＞ T polymorphic sites are related to the character of pig up to 100kg weight ages in days,
Using 349 durocs as experiment material, tested as follows：
(1) genomic DNA of the ear-edge tissue of every pig is extracted, carries out PCR according to the method for (three) in step 1 respectively
Amplification, obtains each pcr amplification product, determines that the genotype of every pig is homozygous CC, heterozygosis according to the method for (four) in step 1
CT or homozygosis TT.
(2) age in days when measuring and recording every pig up to 100kg weight.
The results are shown in Table 1.
Table 1 shows that homozygous CC genotype individuals reach 100kg up to 100kg weight age in days numbers or heterozygosis CT genotype individuals
Weight age in days number is more than homozygosis TT genotype individuals and reaches 100kg weight age in days numbers.
(3) SSC7g.34981027C ＞ T sites are associated with point with up to 100kg ages in days character with least square method development
Analysis.
Model used is as follows：
Y=S+W+G+e
Wherein, Y is measures character；S is gender fixed effect；W is preliminary survey weight covariant；G is genotype effects；E is
Random error.
The results are shown in Table 1.
1 pig mutational site SSC7g.34981027C ＞ T genotype of table being associated with up to 100kg weight age in days characters with pig
Note：The different letters of same column subscript represent significant difference (P ＜ 0.05)
Table 1 shows that homozygous CC genotype individuals exist than homozygous TT genotype individuals up in 100kg weight age in days characters
It is about 12.06 days (P ＜ 0.05) up to 100kg weight ages in days.
So in practical pig breeding, to obtain up to the shorter pig of 100kg weight ages in days, it is preferably selected homozygous TT bases
Because the pig of type individual carries out breeding.
Claims (7)
1. a kind of primer pair for expanding the DNA fragmentation containing SSC7g.34981027C ＞ T polymorphic sites；
The SSC7g.34981027C ＞ T polymorphic sites are pig genome (Sscrofa10.2Primary Assembly) 7
Number the 34981027th bit base of chromosome.
2. primer pair according to claim 1, it is characterised in that：The primer pair is as the DNA shown in SEQ ID No.1 points
Son and the DNA molecular composition shown in SEQ ID No.2.
3. a kind of kit for identifying or assisting identification pig to reach 100kg weight ages in days, the kit include claims 1 or 2 institute
The primer pair stated.
4. a kind of method for identifying or assisting identification pig to reach 100kg weight ages in days, this method are as follows：Homozygous CC genotype individuals reach
100kg weight age in days numbers or heterozygosis CT genotype individuals are reached up to 100kg weight ages in days number more than homozygosis TT genotype individuals
100kg weight age in days numbers.
The pig of the homozygosis CC genotype is that the base of SSC7g.34981027C ＞ T polymorphic sites is the pig of C；
It is C and the pig of T that the pig of the heterozygosis CT genotype, which is the base of SSC7g.34981027C ＞ T polymorphic sites,；
The pig of the homozygosis TT genotype is that the base of SSC7g.34981027C ＞ T polymorphic sites is the pig of T；
The SSC7g.34981027C ＞ T polymorphic sites are pig genome (Sscrofa10.2 Primary Assembly) 7
Number the 34981027th bit base of chromosome.
5. according to the method described in claim 4, it is characterized in that：The homozygosis CC genotype, heterozygosis CT genotype or homozygosis
The determination method of TT genotype is as follows：Using the genomic DNA of pig as template, using the primer pair described in claims 1 or 2 as primer
PCR amplification is carried out, pcr amplification product is obtained, if the pig genome (Sscrofa10.2 contained in pcr amplification product
Primary Assembly) No. 7 the 34981027th bit bases of chromosome are C, then and the genotype of the pig is homozygosis CC genes
Type, if base is C and T at this, the genotype of the pig is heterozygosis CT genotype, described if base is T at this
The genotype of pig is homozygosis TT genotype.
6. method according to claim 4 or 5, it is characterised in that：The primer is the primer pair described in claim 2
When, described No. 7 the 34981027th bit bases of chromosome of pig genome (Sscrofa10.2 Primary Assembly) are located at institute
State pcr amplification product the 169th from 5 ' ends.
7. the kit described in primer pair, claim 3 and/or claim 4-6 described in claims 1 or 2 are any described
Application of the method in the selection and breeding of pig.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611138579.3A CN108220449B (en) | 2016-12-12 | 2016-12-12 | Method for auxiliary detection of day-age of pigs reaching 100kg body weight and special primer pair thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611138579.3A CN108220449B (en) | 2016-12-12 | 2016-12-12 | Method for auxiliary detection of day-age of pigs reaching 100kg body weight and special primer pair thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108220449A true CN108220449A (en) | 2018-06-29 |
| CN108220449B CN108220449B (en) | 2020-07-10 |
Family
ID=62638863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611138579.3A Expired - Fee Related CN108220449B (en) | 2016-12-12 | 2016-12-12 | Method for auxiliary detection of day-age of pigs reaching 100kg body weight and special primer pair thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108220449B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112251516A (en) * | 2020-10-15 | 2021-01-22 | 上海新农科技股份有限公司 | Method for detecting standard weight day age of pig by using No. 7 chromosome of pig and kit thereof |
| CN113355428A (en) * | 2020-03-04 | 2021-09-07 | 中国农业科学院农业基因组研究所 | SNP (Single nucleotide polymorphism) marker related to day age of up to 100kg body weight and application method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103898107A (en) * | 2014-04-17 | 2014-07-02 | 江西农业大学 | Major SNP (single nucleotide polymorphism) marker influencing growth traits of pigs and application thereof in genetic improvement of productivity of breeding pigs |
| CN104404133A (en) * | 2014-10-20 | 2015-03-11 | 中国农业大学 | SNP marker associated with pig growth rate, and applications thereof |
| CN105316412A (en) * | 2015-11-16 | 2016-02-10 | 中国农业科学院北京畜牧兽医研究所 | Method for identification or auxiliary identification of day age of pig at 100kg weight and special kit of method |
-
2016
- 2016-12-12 CN CN201611138579.3A patent/CN108220449B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103898107A (en) * | 2014-04-17 | 2014-07-02 | 江西农业大学 | Major SNP (single nucleotide polymorphism) marker influencing growth traits of pigs and application thereof in genetic improvement of productivity of breeding pigs |
| CN104404133A (en) * | 2014-10-20 | 2015-03-11 | 中国农业大学 | SNP marker associated with pig growth rate, and applications thereof |
| CN105316412A (en) * | 2015-11-16 | 2016-02-10 | 中国农业科学院北京畜牧兽医研究所 | Method for identification or auxiliary identification of day age of pig at 100kg weight and special kit of method |
Non-Patent Citations (6)
| Title |
|---|
| FAN B ET AL.: "Genome-Wide Association Study Identifies Loci for Body Composition and Structural Soundness Traits in Pigs", 《PLOS ONE》 * |
| JUNG EJ ET AL.: "Genome-wide association analysis identifies quantitative trait loci for growth in a Landrace purebred population", 《ANIMAL GENETICS》 * |
| WANG K ET AL.: ""Genome Wide Association Analysis Reveals New Production Trait Genes in a Male Duroc Population", 《PLOS ONE》 * |
| WU_ABGC: "rs337283866", 《USUC DBSNP》 * |
| 张哲等: "杜洛克猪生长性状全基因组关联分析", 《广东农业科学》 * |
| 赵谦等: "猪重要性状全基因组关联分析的研究进展", 《畜牧兽医学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113355428A (en) * | 2020-03-04 | 2021-09-07 | 中国农业科学院农业基因组研究所 | SNP (Single nucleotide polymorphism) marker related to day age of up to 100kg body weight and application method thereof |
| CN112251516A (en) * | 2020-10-15 | 2021-01-22 | 上海新农科技股份有限公司 | Method for detecting standard weight day age of pig by using No. 7 chromosome of pig and kit thereof |
| CN112251516B (en) * | 2020-10-15 | 2024-04-26 | 上海新农科技股份有限公司 | Method for detecting standard weight and day-age of pigs by using chromosome 7 of pigs and kit thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108220449B (en) | 2020-07-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Schlötterer et al. | Sequencing pools of individuals—mining genome-wide polymorphism data without big funding | |
| CN108728552B (en) | It is a kind of influence duroc eye muscle area character molecular labeling and application | |
| OLANO‐MARIN et al. | A genome‐wide set of 106 microsatellite markers for the blue tit (Cyanistes caeruleus) | |
| CN101824416B (en) | Pork quality trait related gene DGAT1 and application thereof in porcine marker-assisted selection | |
| CN107164463A (en) | It is a kind of to be used for the SNP marker of measure and/or genetic improvement pig growth traits | |
| CN106086195A (en) | One SNP marker relevant to the cabrilla speed of growth and application thereof | |
| CN108531615A (en) | One breeder HS6ST3 genes 43bp indel polymorphism marks and its application, detection primer, kit | |
| CN103146829B (en) | Method for assistant selection of goose muscle fatty acid performances by utilizing molecular markers | |
| Mascolo et al. | Comparison of mitochondrial DNA enrichment and sequencing methods from fish tissue | |
| CN104672315B (en) | Control gene and to Cucumber Roll palpus character related SNP marker of the cucumber without tendril character | |
| CN109680070A (en) | It is a kind of to the extremely significant relevant SNP marker of the white sheep hoof color in Australia, molecular labeling and application | |
| CN104480108B (en) | Method for identifying pig back fat thickness and special primer pair thereof | |
| CN106498048B (en) | One kind QTL relevant to soybean nodulation number, SNP marker and application | |
| CN109182557B (en) | SNP molecular marker for identifying low dissolved oxygen tolerance and fullness of pelteobagrus vachelli and application thereof | |
| CN112126691B (en) | Method, primer, kit and application for auxiliary detection of pig backfat thickness character by using pig chromosome 2 gene polymorphism site | |
| CN108220449A (en) | A kind of method and its special primer pair that detection pig is assisted to reach 100kg weight ages in days | |
| CN104131097B (en) | A kind of method detecting beef cattle UCP3 gene mononucleotide polymorphism and application thereof | |
| CN108359733B (en) | Method for auxiliary detection of 100kg backfat thickness of pig | |
| CN107475414B (en) | Method for screening parent oysters with high glycogen content | |
| CN106048028A (en) | Grouper-growth-rate-related SNP (single-nucleotide polymorphism) marker and application thereof | |
| CN107988385A (en) | A kind of method and its dedicated kit for detecting beef cattle PLAG1 genes Indel marks | |
| CN104195136B (en) | Method or identifying length and/or height of pig body and special primer pair therefor | |
| CN112575103B (en) | QTL (quantitative trait locus), molecular marker, KASP (Kaposi-specific protein) detection primer group and application for controlling quality traits of single lotus seeds | |
| CN105256045B (en) | It is a kind of identify pig kill after 24 it is small when longissimus dorsi muscle pH value size method and its special primer pair | |
| CN113801947A (en) | SNP (Single nucleotide polymorphism) marker related to growth speed character of basha fish, detection primer and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| DD01 | Delivery of document by public notice |
Addressee: Liu Xin Document name: Notification of Acceptance of Patent Application |
|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200710 Termination date: 20201212 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |