CN108220137A - A kind of tumour cell trap setting - Google Patents
A kind of tumour cell trap setting Download PDFInfo
- Publication number
- CN108220137A CN108220137A CN201810107084.7A CN201810107084A CN108220137A CN 108220137 A CN108220137 A CN 108220137A CN 201810107084 A CN201810107084 A CN 201810107084A CN 108220137 A CN108220137 A CN 108220137A
- Authority
- CN
- China
- Prior art keywords
- film layer
- closed container
- tumour cell
- filtering film
- trap setting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
Abstract
The invention discloses a kind of tumour cell trap settings, it is related to the field of medical instrument technology, including closed container, chamber resettling is formed with inside it, the closed container is disposed with the retention unit for being located at the chamber resettling into blood mouth and blood outlet, the retention unit includes at least one layer of filtering film layer, and each filter membrane layer arrangement has multiple retention micropores.The present invention passes through the closed container equipped with one layer or more filtering film layer in designing, and the retention micropore in filtering film layer retains blood circulation tumour cell, reliable, objective reality Data Detection source object is provided for subsequent detection, pass through the back flush trap setting simultaneously, elute catch, the trap setting easy removal is easily changed, and can be connect with conduit, is applied on various blood catheter accesses.
Description
Technical field
The present invention relates to the field of medical instrument technology, more particularly to a kind of tumour cell trap setting.
Background technology
At present, it although in the existing equipment to blood circulation tumour progress Cell capture of medical field, and is captured
Tumour cell is used for downstream analysis, still, on the one hand, the tumour cell captured still has careless omission, can not reflect to objective reality
The truth of patient, on the other hand, catch is eluted out from existing tumour cell trap setting it is relatively difficult, simultaneously
It is easily damaged when eluting catch or damaged portion catch, the tumour cell detection of next step can not be given to provide objective reality
Detectable substance.Obviously, there is an urgent need for a kind of tumour cells that can be captured to greatest extent in blood for detecting, and reflect to objective trouble
The interior tumor cell situation of person provides instruction for doctor's clinical diagnosis.
Invention content
The purpose of the present invention is to provide a kind of tumour cell trap setting, can intercept to greatest extent swollen in blood
Oncocyte.
Used technical solution to solve above-mentioned technical problem：
A kind of tumour cell trap setting, including
Closed container, inside be formed with chamber resettling, which is disposed with into blood mouth and blood outlet
Positioned at the retention unit of the chamber resettling, which includes at least one layer of filtering film layer, each filtering
Film layer is disposed with multiple retention micropores.
As a further improvement of the above technical scheme, the inner wall at the edge and the closed container of each filtering film layer
It is tightly connected, there is the connecting portion being connect with each filtering film layer in the closed container.
As a further improvement of the above technical scheme, it is described enter blood mouth be connected with the entrance connecting pin of hollow tube-shape, institute
State the outlet connecting pin that blood outlet is connected with hollow tube-shape.By being connected in the trap setting inlet porting connecting pin and outlet
End so that the trap setting can be connect with conduit, and be applied on various blood catheter accesses, in addition, the trap setting has
Easy removal, the features such as being easily changed.
As a further improvement of the above technical scheme, at least three layers of the film layer of filtering, including the first film layer, the second film
Layer and third membrane layer are disposed with the clearance space of transition between each filtering film layer.
As a further improvement of the above technical scheme, the retention micropore size of first film layer is 45 μm to 80 μm institutes
The retention micropore size for stating the second film layer is 25 μm to 50 μm, and the retention micropore size of the third membrane layer is 10 μm to 35 μm.
As a further improvement of the above technical scheme, the closed container is in pie, and each filtering film layer is in flat
Planar, the side of each filtering film layer towards the blood outlet are disposed with the stent of reticular structure.
As a further improvement of the above technical scheme, the closed container includes the first gland and close with first gland
The second gland of connection is sealed, the lateral wall of first gland has the holding section extended radially out, second gland
Madial wall has the snap fit portion radially-inwardly stretched out.Further, retainer ring of each connecting portion for annular shape, each institute
It states retainer ring upper and lower ends face and is arranged with the sealing ring pressed and touched accordingly filtering film layer or closed container inner side end.By using
The technical solution of the structure can improve the producing efficiency of trap setting, first each filtering film layer is mounted in the first gland, so
After cover the second gland, the second gland by holding section auxiliary section thereon it is seizing live the first gland on holding section, and pass through
Sealing ring on each retainer ring forms the chamber resettling described in inside.
As an alternative to the above-described technical solution, the closed container is cylindrical in shape, the upper surface of the closed container
Be arranged with multiple annular flanges being sequentially increased on the inside of inside and lower face, the annular flange of the closed container upper surface and
The corresponding annular flange in lower face forms the connecting portion, and each filtering film layer is cylindrical in shape, each described filtering film layer one end
It is embedded in the annular flange of the closed container upper surface, it is annular accordingly that the other end is embedded in the closed container lower face
In flange.
As a further improvement of the above technical scheme, first film layer, the second film layer and third membrane layer by outer and
Inside be sequentially arranged in the closed container, it is described enter blood mouth at be disposed with flow-guiding channel to the outside of first film layer.
The beneficial effects of the invention are as follows：The closed container that the present invention filters film layer by being equipped at least one layer in designing, and
The tumour cell in blood is retained by the retention micropore filtered in film layer, is provided for subsequent detection reliable, objective true
Real Data Detection source object, while by the back flush trap setting, catch is eluted, and the trap setting is dismantled
It is simple and convenient, be easily changed, can connect, be applied on various blood catheter accesses with conduit.
Description of the drawings
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described.Obviously, described attached drawing is the part of the embodiment of the present invention rather than all implements
Example, those skilled in the art without creative efforts, can also be obtained according to these attached drawings other designs
Scheme and attached drawing.
Fig. 1 is the structure diagram of the first embodiment of the invention；
Fig. 2 is the structure diagram of second of embodiment of the invention.
Specific embodiment
The technique effect of the design of the present invention, concrete structure and generation is carried out below with reference to embodiment and attached drawing clear
Chu is fully described by, to be completely understood by the purpose of the present invention, feature and effect.Obviously, described embodiment is this hair
Bright part of the embodiment rather than whole embodiments, based on the embodiment of the present invention, those skilled in the art is not paying
The other embodiment obtained under the premise of creative work, belongs to the scope of protection of the invention.In addition, be previously mentioned in text
All connection/connection relations not singly refer to component and directly connect, and refer to be added deduct by adding according to specific implementation situation
Few couple auxiliary, to form more preferably coupling structure.
With reference to Fig. 1, a kind of tumour cell trap setting, including closed container 1 and retention unit, inside closed container 1 into
Xing You chamber resettling, retention unit are located at chamber resettling, and retention unit includes at least one layer of filtering film layer, and each film layer that filters is gathered
Multiple retention micropores.The closed container 1 of present embodiment is in pie, and respectively filtering film layer is in planar, and preferably three layers,
Respectively the first film layer 4, the second film layer 5 and third membrane layer 6 are respectively disposed with the clearance space of transition between filtering film layer,
In the first embodiment, the clearance space of transition is round pie, and each side for filtering film layer towards blood outlet is disposed with netted
The stent 7 of structure, wherein there are two stents 7 to be located at the clearance space between two filtering film layers, another stent 7 is located at tertiary membrane
Between layer 6 and 1 inner wall of closed container, in blood stream after previous filtering film layer, by clearance space, next mistake is passed through
Filter membranous layer can prevent two layers of filter membrane stacking to be susceptible to the situation of blocking after closing, by the transition of clearance space, make blood
The retention micropore in each filtering film layer can uniformly be reached.Meanwhile first film layer 4 retention micropore size for 45 μm to 80 μm,
The retention micropore size of second film layer 5 is 25 μm to 50 μm, and the retention micropore size of third membrane layer 6 is 10 μm to 35 μm.At this
In embodiment, the retention micropore size of the first film layer 4 is preferably 60 μm, and the retention micropore size of the second film layer 5 is preferably 41 μ
M, the retention micropore size of third membrane layer 6 is preferably 30 μm, and the inner wall sealing at each edge for filtering film layer and closed container 1 connects
It connects, there is the connecting portion 8 being connect with each filtering film layer in closed container 1.
Closed container 1, which is disposed with, simultaneously into blood mouth and blood outlet, enters the entrance connecting pin that blood mouth is connected with hollow tube-shape
2, blood outlet is connected with the outlet connecting pin 3 of hollow tube-shape.Blood enters the trap setting of the present invention through entering blood mouth, followed by
It is flowed out after first film layer 4, the second film layer 5 and third membrane layer 6 through blood outlet.By filtering film layer three times, can catch to greatest extent
The tumour cell in blood is grasped, by the back flush trap setting, elutes catch, is provided for subsequent detection reliable
, the Data Detection source object of objective reality, and the trap setting easy removal, be easily changed, can be connect with conduit,
And it is applied on various blood catheter accesses.
As being further improved for the first embodiment, closed container 1 include the first gland 11 and with first gland
11 the second glands 12 being tightly connected, the lateral wall of the first gland 11 have the holding section extended radially out, the second gland 12
Madial wall there is the snap fit portion radially-inwardly stretched out.Further, each connecting portion 8 is circular retainer ring, each to block
Circle upper and lower ends face is arranged with pressure and touches in the accordingly sealing ring of filtering film layer or closed container inner side end.First by each filter membrane
Layer is mounted in the first gland 11, then covers the second gland 12, the second gland 12 is seizing by holding section auxiliary section thereon
The firmly holding section on the first gland 11, and pass through the sealing ring on each retainer ring, form the internal chamber resettling.
Fig. 2 illustrates second of embodiment of the present invention, with the first embodiment difference, is：Closed appearance
Device 1 is cylindrical in shape, and multiple annular flanges being sequentially increased are arranged on the inside of the upper surface of closed container 1 and on the inside of lower face,
The corresponding annular flange composition connecting portion 8 of the annular flange of 1 upper surface of closed container and lower face, filtering film layer are cylindrical in shape,
In second of embodiment, the clearance space of transition is embedded in hollow tube-shape, each film layer one end of filtering between each filtering film layer
In the annular flange of 1 upper surface of closed container, the other end is embedded in the corresponding annular flange in 1 lower face of closed container.First
Film layer 4, the second film layer 5 and third membrane layer 6 are sequentially arranged in from outside to inside in closed container 1, i.e., each to filter the effective of film layer
It is different to retain area, meanwhile, enter to be disposed with the outside of 9 to the first film layer 4 of flow-guiding channel at blood mouth.Blood is entered through entering blood mouth
Then trap setting flows to the outside of the first film layer 4 by flow-guiding channel 9, the specific stream inside tubular closed container 1
To, be from flowing to blood outlet inward outside, i.e., followed by the first film layer 4, the second film layer 5 and the third membrane layer 6 in Fig. 2,
After flowing through previous filtering film layer, by being in the clearance space of hollow tube-shape, next filtering film layer is passed through, can effectively be kept away
Exempt from the situation of filtering film block.In the second embodiment, the retention micropore size of the first film layer 4 is preferably 70 μm, the
The retention micropore size of two film layers 5 is preferably 50 μm, and the retention micropore size of third membrane layer 6 is preferably 30 μm.
Embodiments of the present invention are explained in detail above in conjunction with attached drawing, but the present invention is not limited to above-mentioned embodiment party
Formula, can also be before present inventive concept not be departed from the knowledge having in the technical field those of ordinary skill
Put that various changes can be made.
Claims (10)
1. a kind of tumour cell trap setting, it is characterised in that：Including
Closed container (1), inside be formed with chamber resettling, which is disposed with into blood mouth and blood outlet；
Positioned at the retention unit of the chamber resettling, which includes at least one layer of filtering film layer, each filtering film layer
It is disposed with multiple retention micropores.
2. tumour cell trap setting according to claim 1, it is characterised in that：The edge of each filtering film layer and institute
The inner wall sealing connection of closed container (1) is stated, there is the connecting portion being connect with each filtering film layer in the closed container (1)
(8)。
3. tumour cell trap setting according to claim 2, it is characterised in that：It is described enter blood mouth be connected with hollow tube-shape
Entrance connecting pin (2), the blood outlet is connected with the outlet connecting pin (3) of hollow tube-shape.
4. the tumour cell trap setting according to claims 1 or 2 or 3, it is characterised in that：The filtering film layer at least three
Including the first film layer (4), the second film layer (5) and third membrane layer (6), transition is disposed between each filtering film layer for layer
Clearance space.
5. tumour cell trap setting according to claim 4, it is characterised in that：The retention of first film layer (4) is micro-
Hole aperture is 45 μm to 80 μm, and the retention micropore size of second film layer (5) is 25 μm to 50 μm, the third membrane layer (6)
Retention micropore size be 10 μm to 35 μm.
6. tumour cell trap setting according to claim 4, it is characterised in that：The closed container (1) is in pie, respectively
The filtering film layer is in planar, and the side of each filtering film layer towards the blood outlet is disposed with the stent (7) of reticular structure.
7. tumour cell trap setting according to claim 6, it is characterised in that：The closed container (1) is including first
Gland (11) and the second gland being tightly connected with first gland (12), the lateral wall of first gland (11) have radially
Outwardly directed holding section, the madial wall of second gland (12) have the snap fit portion radially-inwardly stretched out.
8. tumour cell trap setting according to claim 7, it is characterised in that：Each connecting portion (8) is annular shape
Retainer ring, each retainer ring upper and lower ends face be arranged with pressure touch accordingly filtering film layer or closed container inner side end sealing
Circle.
9. tumour cell trap setting according to claim 4, it is characterised in that：The closed container (1) is cylindrical in shape, institute
It states on the inside of the upper surface of closed container (1) and is arranged with multiple annular flanges being sequentially increased on the inside of lower face, it is described closed
The corresponding annular flange of the annular flange of container (1) upper surface and lower face forms the connecting portion (8), and the filtering film layer is equal
It is cylindrical in shape, each described filtering film layer one end is embedded in the corresponding annular flange in the closed container (1) upper surface, and the other end is embedding
Enter in the corresponding annular flange in the closed container (1) lower face.
10. tumour cell trap setting according to claim 9, it is characterised in that：First film layer (4), the second film
Layer (5) and third membrane layer (6) are sequentially arranged in from outside to inside in the closed container (1), it is described enter blood mouth at be disposed with and lead
The outside of circulation road (9) extremely first film layer (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810107084.7A CN108220137A (en) | 2018-02-02 | 2018-02-02 | A kind of tumour cell trap setting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810107084.7A CN108220137A (en) | 2018-02-02 | 2018-02-02 | A kind of tumour cell trap setting |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108220137A true CN108220137A (en) | 2018-06-29 |
Family
ID=62670363
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810107084.7A Pending CN108220137A (en) | 2018-02-02 | 2018-02-02 | A kind of tumour cell trap setting |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108220137A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6177019B1 (en) * | 1996-08-26 | 2001-01-23 | Hemasure Inc. | Method and apparatus for removing tumor cells from tumor cell-contaminated stem cell products |
| WO2012119646A1 (en) * | 2011-03-08 | 2012-09-13 | Kentsch Michael | Method for reducing the risk of metastasis formation in cancerous diseases, and system for removing tumour cells from the blood circulation |
| CN103599574A (en) * | 2013-11-22 | 2014-02-26 | 武汉友芝友医疗科技有限公司 | Filter for isolating circulating tumor cells |
| CN204484884U (en) * | 2014-12-26 | 2015-07-22 | 北京中科盛康科技有限公司 | A kind of blood filter |
| CN208218851U (en) * | 2018-02-02 | 2018-12-11 | 广州益养生物科技有限公司 | A kind of tumour cell capture device |
-
2018
- 2018-02-02 CN CN201810107084.7A patent/CN108220137A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6177019B1 (en) * | 1996-08-26 | 2001-01-23 | Hemasure Inc. | Method and apparatus for removing tumor cells from tumor cell-contaminated stem cell products |
| WO2012119646A1 (en) * | 2011-03-08 | 2012-09-13 | Kentsch Michael | Method for reducing the risk of metastasis formation in cancerous diseases, and system for removing tumour cells from the blood circulation |
| CN103599574A (en) * | 2013-11-22 | 2014-02-26 | 武汉友芝友医疗科技有限公司 | Filter for isolating circulating tumor cells |
| CN204484884U (en) * | 2014-12-26 | 2015-07-22 | 北京中科盛康科技有限公司 | A kind of blood filter |
| CN208218851U (en) * | 2018-02-02 | 2018-12-11 | 广州益养生物科技有限公司 | A kind of tumour cell capture device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208032060U (en) | Novel pin hair style blood filter | |
| CN206252701U (en) | Multiplex washing pipe light proofing infusion apparatus | |
| CN108458964A (en) | A kind of antibody compositions and its application in the detection of lymphoma lymphoplasmacytic minimal residual | |
| CN208218851U (en) | A kind of tumour cell capture device | |
| CN201492445U (en) | Muddy stone attraction filter | |
| CN108220137A (en) | A kind of tumour cell trap setting | |
| CN109342720A (en) | In body tumor cell enrichment detection device and method | |
| CN205719721U (en) | A kind of Thinprep pap test film-making filter membrane cylinder | |
| CN208769836U (en) | A kind of extracorporal circulatory system Cell capture system for making liquid biopsy | |
| CN109234152A (en) | A kind of circulating tumor cell separator | |
| CN208598348U (en) | A kind of endoscopy excreta barrier cover of buttocks | |
| CN205460062U (en) | Thorax hydrops collection device | |
| CN205286375U (en) | Double cannula centrifugation syringe | |
| CN208552607U (en) | New-type splanchnocoel drainage device | |
| CN208525564U (en) | A kind of precision blood filter device | |
| CN209060178U (en) | Multi-functional chemotherapy tube flushing device | |
| CN205885962U (en) | Drainage tube is used in thorax operation | |
| CN106581808B (en) | Can heat can be vented stop liquid precision filter infusion set without adjacent benzene | |
| CN108721961A (en) | A kind of filter device of blood | |
| CN108175448A (en) | A kind of extracorporal circulatory system Cell capture system for making liquid biopsy | |
| CN202061163U (en) | Micropore blood returning preventing infusion device | |
| CN209751829U (en) | Light-shading infusion apparatus | |
| CN205108483U (en) | Conveniently extract drainage device of hydrothorax sample | |
| CN208301915U (en) | Dialysate filter device | |
| CN209456433U (en) | It is a kind of for biomolecule or the acquisition equipment of cell |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |