CN108175448A - A kind of extracorporal circulatory system Cell capture system for making liquid biopsy - Google Patents
A kind of extracorporal circulatory system Cell capture system for making liquid biopsy Download PDFInfo
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- CN108175448A CN108175448A CN201810107737.1A CN201810107737A CN108175448A CN 108175448 A CN108175448 A CN 108175448A CN 201810107737 A CN201810107737 A CN 201810107737A CN 108175448 A CN108175448 A CN 108175448A
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- 238000011528 liquid biopsy Methods 0.000 title claims abstract description 21
- 210000004369 blood Anatomy 0.000 claims abstract description 171
- 239000008280 blood Substances 0.000 claims abstract description 157
- 210000004027 cell Anatomy 0.000 claims abstract description 46
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 claims abstract description 26
- 230000017531 blood circulation Effects 0.000 claims abstract description 18
- 230000000740 bleeding effect Effects 0.000 claims abstract description 14
- 230000004087 circulation Effects 0.000 claims abstract description 12
- 238000001914 filtration Methods 0.000 claims description 23
- 230000014759 maintenance of location Effects 0.000 claims description 18
- 239000012528 membrane Substances 0.000 claims description 13
- 210000004881 tumor cell Anatomy 0.000 claims description 8
- 230000002441 reversible effect Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 3
- 210000004907 gland Anatomy 0.000 description 10
- 230000006872 improvement Effects 0.000 description 7
- 230000000903 blocking effect Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 210000004204 blood vessel Anatomy 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 238000001802 infusion Methods 0.000 description 4
- 238000012544 monitoring process Methods 0.000 description 4
- 239000004433 Thermoplastic polyurethane Substances 0.000 description 3
- 210000004165 myocardium Anatomy 0.000 description 3
- 210000005241 right ventricle Anatomy 0.000 description 3
- 229920002803 thermoplastic polyurethane Polymers 0.000 description 3
- 230000007704 transition Effects 0.000 description 3
- 238000003759 clinical diagnosis Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229920001971 elastomer Polymers 0.000 description 2
- 210000002837 heart atrium Anatomy 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000806 elastomer Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 210000001365 lymphatic vessel Anatomy 0.000 description 1
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- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000004088 pulmonary circulation Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- 210000002620 vena cava superior Anatomy 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/38—Removing constituents from donor blood and storing or returning remainder to body, e.g. for transfusion
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/09—Body tissue
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- Health & Medical Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Vascular Medicine (AREA)
- Biomedical Technology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
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- Surgery (AREA)
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Abstract
The invention discloses a kind of extracorporal circulatory system Cell capture systems for making liquid biopsy, it is related to the field of medical instrument technology, including taking blood conduit, blood back conduit and extracorporeal circulation apparatus, the extracorporeal circulation apparatus includes blood pump, connects the blood circulation conduit of the blood pump and the Cell capture device for retaining target cell in blood, the Cell capture device is arranged in blood circulation conduit, the blood circulation conduit include taking described in connection blood conduit into blood conduit and the bleeding conduit for connecting the blood back conduit.The extracorporal circulatory system Cell capture system of the present invention can be used for liquid biopsy, by the system, on the one hand can as far as possible ensure when capturing target cell unaffected to the circulatory function of user；On the other hand, after Cell capture device being designed to circulating tumor cell trap setting, the circulating tumor cell that can be truncated to more Clinical significance of MG is used for downstream analysis.
Description
Technical field
The present invention relates to the field of medical instrument technology, more particularly to a kind of extracorporal circulatory system Cell capture for making liquid biopsy
System.
Background technology
It is tested and analyzed after carrying out Cell capture to the blood of acquisition, has important meaning to guiding clinical diagnosis treatment
Justice.But collected blood is usually peripheral blood at present, due to being filtered by pulmonary circulation and body circulation capillary and lymphatic vessel,
Real conditions can not comprehensively be reacted.For tumour cell, for the first time into sanguimotor tumour cell
Analysis, the more directive significance of clinical conditions.Therefore, need a kind of extracorporal circulatory system Cell capture system can capture first
It is secondary to enter sanguimotor tumour cell, for liquid biopsy.
Invention content
The purpose of the present invention is to provide a kind of extracorporal circulatory system Cell capture system for making liquid biopsy, can capture
Enter sanguimotor tumour cell for the first time, for downstream analysis, while do not influence the blood circulation function of user.
Used technical solution to solve above-mentioned technical problem：
A kind of extracorporal circulatory system Cell capture system for making liquid biopsy, including taking blood conduit, blood back conduit and external
Circulator, the extracorporeal circulation apparatus include blood pump, connect the blood circulation conduit of the blood pump and for retaining in blood
The circulating tumor cell trap setting of target cell, the circulating tumor cell trap setting are arranged in blood circulation conduit, institute
State blood circulation conduit include taking described in connection blood conduit into blood conduit and the bleeding conduit for connecting the blood back conduit.Pass through
Using the technical solution, the target cell that can be truncated on the access that blood circulation conduit is formed in blood can be used for
Downstream analysis.
As a further improvement of the above technical scheme, it further includes and takes blood blood back integrated apparatus, this takes blood blood back integrated
Makeup is put similar to conventional ductus venosus, this takes blood blood back integrated apparatus to include the tube body that one end is tip, described that blood is taken to lead
Pipe and blood back conduit are arranged in the tube body so that the tubular body forms and mutually independent takes haemocoele and blood back cavity, the pipe
Body tip is disposed with the blood back hole for connecting the blood back cavity, and the tube wall is disposed with described in several connections and takes taking for haemocoele
Blood hole.Further, it is described to take blood hole at least three, each position interlaced arrangement for taking blood hole.By being set in tube body
Two-chamber makes to take blood or blood back that can all realize by single tube body, reduces the operation to user, in addition, by designing multiple take
Blood hole, it is possible to prevente effectively from single hole design is susceptible to absorption vascular wall or heart wall and leads to situation about blocking.
As a further improvement of the above technical scheme, the one end of the tube body far from tip is connected with more siphunculus seats, should
More siphunculus seats be connected with take haemocoele described in connection take blood branch pipe, which is connected with the blood back branch for connecting the blood back cavity
Pipe.Further, it is described into blood conduit by taking blood branch pipe described in the connection of the first female Luer, the bleeding conduit passes through the
Two female Luers connect the blood back branch pipe.
As a further improvement of the above technical scheme, the circulating tumor cell trap setting includes closed container and position
In the retention unit in the closed container, which is disposed with into blood mouth and blood outlet, which includes at least one
Layer filtering film layer, each filter membrane layer arrangement have multiple retention micropores.
As a further improvement of the above technical scheme, the inner wall at the edge and the closed container of each filtering film layer
It is tightly connected, there is the connecting portion being connect with each filtering film layer in the closed container.
As a further improvement of the above technical scheme, it is described enter blood mouth be connected with the entrance connecting pin of hollow tube-shape, institute
State the outlet connecting pin that blood outlet is connected with hollow tube-shape.
As a further improvement of the above technical scheme, the circulating tumor cell trap setting is arranged in bleeding conduit,
The bleeding conduit is disposed with around the bypass manifold of the circulating tumor cell trap setting, which is disposed with insurance
Valve, the blood back conduit are disposed with the second pressure sensor for controlling the easing valve open/close states.
As a further improvement of the above technical scheme, it is described to be disposed with first pressure sensor into blood conduit, described
The signal of one pressure sensor feedback controls forward and reverse action of the blood pump.
The beneficial effects of the invention are as follows：Extracorporal circulatory system Cell capture system of the present invention design with Cell capture device,
In use, blood circulation access is formed by extracorporeal circulation apparatus, and passes through Cell capture device and be truncated to for the first time
Into sanguimotor target cell, it on the one hand can ensure the circulatory function to user as far as possible when capturing target cell
Unaffected, on the other hand, the target cell that can be truncated to tool Clinical significance of MG is used for downstream analysis.
Description of the drawings
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described.Obviously, described attached drawing is the part of the embodiment of the present invention rather than all implements
Example, those skilled in the art without creative efforts, can also be obtained according to these attached drawings other designs
Scheme and attached drawing.
Fig. 1 is the structure diagram of the present invention；
Fig. 2 is the first structure chart of circulating tumor cell trap setting in the present invention；
Fig. 3 is second of structure chart of circulating tumor cell trap setting in the present invention；
Fig. 4 is the first structure chart that blood blood back integrated apparatus is taken in the present invention；
Fig. 5 is second of structure chart that blood blood back integrated apparatus is taken in the present invention；
Fig. 6 is the partial structurtes perspective view for second of structure that blood blood back integrated apparatus is taken in the present invention；
Fig. 7 be in Fig. 4 A-A to sectional view；
Fig. 8 is the sectional view of B-B direction in Fig. 5；
Fig. 9 be in Fig. 5 C-C to sectional view.
Reference numeral：1st, blood circulation conduit；2nd, blood pump；3rd, first sensor；4th, circulating tumor cell trap setting；5、
Easing valve；6th, second sensor；7th, bubble detection device；8th, infusion connector；9th, blood blood back integrated apparatus is taken；11st, epicoele is quiet
Arteries and veins, 12, atrium dextrum；13rd, right ventricle；101st, into blood conduit；102nd, go out blood channel；103rd, bypass manifold；104th, first public Rule
Connector；105th, the second public female Luer；401st, closed container；402nd, entrance connecting pin；403rd, connecting pin is exported；404th, the first film
Layer；405th, the second film layer；406th, third membrane layer；407th, stent between film；408th, connecting portion；409th, flow-guiding channel；411st, the first pressure
Lid；412nd, the second gland；901st, tube body；902nd, haemocoele is taken；903rd, blood back cavity；904th, first electrode chamber；905th, second electrode chamber；
906th, first electrode；907th, second electrode；908th, blood hole is taken；910th, blood back hole；911st, more siphunculus seats；912nd, blood branch pipe is taken；
913rd, blood back branch pipe；914th, fixing bracket；915th, the first female Luer connector；916th, the second female Luer connector；991st, the first side opening；
992nd, the second side opening.
Specific embodiment
The technique effect of the design of the present invention, concrete structure and generation is carried out below with reference to embodiment and attached drawing clear
Chu is fully described by, to be completely understood by the purpose of the present invention, feature and effect.Obviously, described embodiment is this hair
Bright part of the embodiment rather than whole embodiments, based on the embodiment of the present invention, those skilled in the art is not paying
The other embodiment obtained under the premise of creative work, belongs to the scope of protection of the invention.In addition, be previously mentioned in text
All connection/connection relations not singly refer to component and directly connect, and refer to be added deduct by adding according to specific implementation situation
Few couple auxiliary, to form more preferably coupling structure.
With reference to Fig. 1, a kind of extracorporal circulatory system Cell capture system for making liquid biopsy, including taking blood conduit, blood back conduit
And extracorporeal circulation apparatus.
With reference to Fig. 1 and Fig. 4, which, which further includes, takes blood blood back integrated apparatus 9, this takes blood to return
Blood integrated apparatus 9 includes the tube body 901 that one end is tip, blood conduit and blood back conduit is taken to be arranged in tube body 901, so as to make
901 inside formation of tube body is mutually independent to take haemocoele 902 and blood back cavity 903, and it is along tube body to take haemocoele 902 and blood back cavity 903
901 length directions arrange that 901 tip of tube body is disposed with the blood back hole 910 of connection blood back cavity 903, if 901 side wall of tube body is disposed with
What dry connection took haemocoele 902 takes blood hole 908.Blood hole 908 at least three is taken, respectively takes the position interlaced arrangement in blood hole 908, certainly
1 side wall local setting of tube body can also be reticulated to structure replacement and take blood pore structure more, purpose is for avoiding tube body pipe 901
Block, when one of them take blood hole 908 be fitted in wall of the heart either blood vessel, blockings will be easy to cause, if block up
Plug is easy for that conduit is caused to form negative pressure, and tube body 901 is caused to adsorb in wall of the heart either blood vessel.It is multiple due to arranging
Take blood hole 908, at this time other interlaced arrangements take blood hole 908 equally connection take haemocoele 902, avoid blocking, also reduce took a blood sample
The risk that the negative pressure that journey is formed damages wall of the heart or blood vessel.With reference to Fig. 7, section of haemocoele 902 and blood back cavity 903 is taken
Face is semicircle.Haemocoele 902 can also will be taken to be arranged to circle certainly, and blood back cavity 903 be arranged to it is sickle-shaped, it is sickle-shaped
Recess direction takes haemocoele 902；Blood back cavity 903 is arranged to circle by suppression, and it is sickle-shaped that haemocoele 902 is taken to be arranged to.Fig. 4 shows
The first frame mode of blood blood back integrated apparatus 9 is taken, and Fig. 5 and Fig. 6 illustrate and take the of blood blood back integrated apparatus 9
Two kinds of frame modes, second frame mode with the first it is maximum difference lies in：Second structure takes blood blood back integrated
Device 9 also arranges first electrode chamber 905 and second electrode chamber 906 in tube body 901, equally, first electrode chamber 905 and second
Electrode cavity 906 is arranged in tube body 901 and along 901 length direction of tube body.With reference to Fig. 5, Fig. 6, Fig. 8 and Fig. 9,901 side of tube body
Wall is disposed with the first side opening 991 of connection first electrode chamber 904, and 901 side wall of tube body is disposed with the of connection second electrode chamber 905
Two side openings 992.First electrode chamber 904 is placed with the first electrode device 906 for monitoring myocardium current potential, first electrode device
906 be strip shaped electric poles, and first electrode device 906 touches heart blood by the first side opening 991, can monitor myocardium electricity
Position, then external monitoring system conduct electric potential signal, and the potential change that by heartbeat when generates intuitively is reflected in monitoring system
On, it is checked convenient for medical personnel located.Second electrode chamber 905 is placed with the second electrode device 907 for cardiac pacing, and second
Electrode assembly 907 is strip shaped electric poles, and 907 external pacemaker of second electrode device uses as pacing electrode, can conduct pacemaker
The electric pulse that system is sent out is applied to cardiac muscle, it is made to be shunk by preset frequency, restores the blood-pumping function of heart.Tube body 901 is separate
The one end at tip is connected with more siphunculus seats 911, and what which was connected with that connection takes haemocoele 902 takes blood branch pipe 912, should
More siphunculus seats 911 are connected with the blood back branch pipe 913 of connection blood back cavity 903.
With reference to Fig. 1, extracorporeal circulation apparatus includes blood pump 2, connects the blood circulation conduit 1 of the blood pump 2 and be arranged in this
The Cell capture device of blood circulation conduit 1, in the present embodiment, Cell capture device are specifically that circulating tumor cell captures
Device 4 can adjust the extracorporal circulatory system Cell capture system whole blood flow velocity, circulating tumor cell by controlling blood pump 2
Trap setting 4 can retain the circulating tumor cell in blood.Blood circulation conduit 1 include connection take blood conduit into blood conduit
101 with the bleeding conduit 102 that connects blood back conduit, i.e., blood branch pipe 912 is taken by the connection of the first female Luer into blood conduit 101,
Bleeding conduit 102 connects blood back branch pipe 913 by the second female Luer.With reference to Fig. 1 and Fig. 4, the first female Luer includes connection
The first female Luer connector of 912 end of blood branch pipe is taken in the first public female Luer 104 into 101 end of blood conduit and being connected to
915, the second female Luer includes the second public female Luer 105 for being connected to 102 end of bleeding conduit and is connected to blood back branch pipe
Second female Luer connector 916 of 913 ends.First pressure sensor 3 is disposed with into blood conduit 101, first pressure sensor 3 is anti-
Forward and reverse action of the signal control blood pump 2 of feedback.When tube body 1 is when taking blood hole 908 to block, first pressure sensor 3 is examined
Negative pressure is measured, when negative pressure persistently exists and reaches the pressure value of setting, 3 feedback signal of first pressure sensor is to blood pump 2, blood pump
2 it is of short duration it is reversed operate so that make circulation conduit 1 and take blood blood back integrated apparatus 9 generate trembling, make the tube body 901 of blocking voluntarily
Conducting, it is ensured that the safety and reliability of entire extracorporal circulatory system.
Specifically, with reference to Fig. 2, circulating tumor cell trap setting 4 includes closed container 401 and positioned at the closed container
Retention unit in 401, the closed container 401 are disposed with into blood mouth and blood outlet, which includes at least one layer of filter
Film layer, each filter membrane layer arrangement have multiple retention micropores.With reference to Fig. 2, circulating tumor cell trap setting 4 is in pie, each mistake
Filter membranous layer is in planar, and preferably three layers, respectively the first film layer 404, the second film layer 405 and third membrane layer 406, respectively
The clearance space of transition is disposed between filtering film layer, each side for filtering film layer towards blood outlet is disposed with the film of reticular structure
Between stent 407, wherein be located at the clearance space between two filtering film layers there are two stent 407 between film, stent 407 between another film
Between 1 inner wall of third membrane layer 406 and closed container, in blood stream after previous filtering film layer, by clearance space, then
Next filtering film layer is flowed through, two layers of filter membrane stacking can be prevented to be susceptible to the situation of blocking after closing, by clearance space
Transition, allow blood uniformly reach it is each filtering film layer on retention micropore.Meanwhile first film layer 404 retention micropore hole
Diameter is 45 μm to 80 μm, and the retention micropore size of the second film layer 405 is 25 μm to 50 μm, the retention micropore hole of third membrane layer 406
Diameter is 10 μm to 35 μm.In the first frame mode of circulating tumor cell trap setting 404, the retention of the first film layer 404
Micropore size is preferably 60 μm, and the retention micropore size of the second film layer 405 is preferably 41 μm, the retention micropore of third membrane layer 406
Aperture is preferably 30 μm, and each edge for filtering film layer is connect with the inner wall sealing of closed container 401, is had in closed container 401
The connecting portion 408 being connect with each filtering film layer.Closed container 401 includes the first gland 411 and seals with first gland 411 to connect
The second gland 412 connect, the lateral wall of the first gland 411 have the holding section extended radially out, the inside of the second gland 412
Wall has the snap fit portion radially-inwardly stretched out.Further, retainer ring of each connecting portion 8 for annular shape, each retainer ring
Pressure is touched in filtering film layer or closed container inner side end accordingly after upper and lower ends face is arranged with sealing ring.During assembling, first will
Each filtering film layer is mounted in the first gland 411, then covers the second gland 412, the second gland 412 passes through holding section thereon
Holding section on seizing firmly the first gland 411 in auxiliary section.Enter the entrance connecting pin 402 that blood mouth is connected with hollow tube-shape, blood outlet
It is connected with the outlet connecting pin 403 of hollow tube-shape.Blood enters circulating tumor cell trap setting 4 through entering blood mouth, followed by
It is flowed out after first film layer 404, the second film layer 405 and third membrane layer 406 through blood outlet.It, can be maximum by filtering film layer three times
Limit captures the tumour cell in blood, by the back flush circulating tumor cell trap setting 4, elutes catch,
Reliable, objective reality Data Detection source object is provided for subsequent detection, available for downstream analysis, such as genetic analysis,
In vitro culture, drug sensitive test etc., the data of monitoring are treated available for guiding clinical diagnosis, and the circulating tumor cell captures dress
It puts 4 easy removals, be easily changed.
Fig. 3 illustrates second of structure of circulating tumor cell trap setting 4, with the first structure of Fig. 2 difference lies in：
Closed container 401 is cylindrical in shape, and multiple be sequentially increased is arranged on the inside of the upper surface of closed container 401 and on the inside of lower face
Annular flange, the corresponding annular flange composition connecting portion 408 of the annular flange of 401 upper surface of closed container and lower face, filtering
Film layer is cylindrical in shape, and in second of structure, the clearance space of transition is each to filter film layer one in hollow tube-shape between each filtering film layer
End is embedded in the annular flange of 401 upper surface of closed container, and it is annular accordingly that the other end is embedded in 401 lower face of closed container
In flange.First film layer 404, the second film layer 405 and third membrane layer 406 are sequentially arranged in from outside to inside in closed container 401,
The effectively catching area of i.e. each filtering film layer is different, meanwhile, enter to be disposed with the outer of 9 to the first film layer 404 of flow-guiding channel at blood mouth
Side.Blood is to enter trap setting through entering blood mouth, and the outside of the first film layer 404 is then flowed to by flow-guiding channel 409, from
In the closed container 401 of tubular, from blood outlet is flowed to inward outside, i.e., followed by the first film layer 404, the second film layer 405 and
Third membrane layer 406 after previous filtering film layer is flowed through, by being in the clearance space of hollow tube-shape, passes through next filtering
Film layer, it is possible to prevente effectively from the situation of filtering film block.In the circulating tumor cell trap setting 4 of second of structure, the
The retention micropore size of one film layer 404 is preferably 70 μm, and the retention micropore size of the second film layer 405 is preferably 50 μm, tertiary membrane
The retention micropore size of layer 406 is preferably 30 μm.
With reference to Fig. 1, circulating tumor cell trap setting 4 is arranged on the access of bleeding conduit 102, bleeding conduit 102
The bypass manifold 103 around the circulating tumor cell trap setting 4 is disposed with, which is disposed with easing valve 5, returns
Blood conduit 102 is disposed with the second pressure sensor 6 for controlling 5 open/close states of easing valve.Tumour cell capture device 4 uses one
After the section time, the circulation within the unit interval declines or even blocks, then can be examined by second pressure sensor 6
Pressure increase is measured, if supercharging continues and reach the pressure value of setting, control easing valve 5 is opened, and leads bypass manifold 103
It is logical, it prevents blood circulation from interrupting, avoids the excessive risk that circulation conduit is caused to rupture of blood pressure.
It is that tube body 901 is inserted into heart along superior vena cava 11, such as using the extracorporal circulatory system Cell capture system of the present invention
Shown in Fig. 1,901 front end of tube body is inserted into right ventricle 13, i.e., blood back hole 910 is located at right ventricle 13, and blood hole 908 is taken to be located at
In atrium dextrum 12, it is preferably 1.2mm respectively to take the aperture in blood hole, and taking of all taking that blood hole 908 formed is big after blood regional implementation
It causes rounded.According to the structure of heart, it is 30mm~50mm to take 908 lower edge of blood hole and the distance in blood back hole 910, can prevent from taking
Blood and blood back are obscured.The preferred 10mm of distance at 991 center of the first side opening and blood back hole 910；Second side opening, 992 center and blood back hole
The 10 preferred 10mm of distance.To avoid the damage to blood vessel and heart, conduit preferably uses TPU (Thermoplastic
Polyurethanes, thermoplastic polyurethane elastomer rubber) material.Meanwhile it with reference to Fig. 4, is arranged in more 911 both sides of siphunculus seat
There is fixing bracket 914, each fixing bracket 914 is disposed with aperture, can be fixed by having the foraminate suture of fixing bracket 914.Or
Person is with reference to Fig. 5, and there are two after aperture, more 911 shells of siphunculus seat play and 914 phase of fixing bracket more 911 shell of siphunculus seat arrangements
Same function, more siphunculus seats 911 are to play more siphunculus road conducting functions and fixing bracket function at this time.It should be noted that this
Embodiment is that extracorporal circulatory system Cell capture system is applied in the extracorporal circulatory system that heart is drawn, and is followed this is because just entering blood
The cell of ring has more the directive significance of clinical conditions.Certainly, those skilled in the art can apply the invention to other
In position, target cell is retained, as liquid biopsy, such as can be adjusted according to the specific configuration of corresponding site and respectively take blood hole 908
With the spacing in blood back hole 910；Also the aperture of each retention micropore according to the concrete structure of target cell, can be accordingly adjusted, to retain it
Its cell, for downstream analysis.
By using the extracorporal circulatory system Cell capture system of the present invention, can effectively intercept for the first time into sanguimotor
Tumour cell, as liquid biopsy.
Preferred embodiment is further used as, bleeding conduit 102 is disposed with bubble detection device 7, bubble detection device 7
Specifically it is arranged at the blood outlet of circulating tumor cell trap setting 4.When the bubble detected is more than setting value, control blood pump
2 make system out of service.
Preferred embodiment is further used as, infusion connector 8 is connected with into blood conduit 101, when user needs to infuse
When, such as injecting normal saline or heparin, it can be from 8 external infusion equipment of infusion connector.
Embodiments of the present invention are explained in detail above in conjunction with attached drawing, but the present invention is not limited to above-mentioned embodiment party
Formula, can also be before present inventive concept not be departed from the knowledge having in the technical field those of ordinary skill
Put that various changes can be made.
Claims (10)
1. a kind of extracorporal circulatory system Cell capture system for making liquid biopsy, it is characterised in that：Including taking blood conduit, blood back conduit
And extracorporeal circulation apparatus, the extracorporeal circulation apparatus include blood pump (2), connect the blood circulation conduit (1) of the blood pump (2) with
And the Cell capture device for retaining target cell in blood, the Cell capture device are arranged in blood circulation conduit (1),
The blood circulation conduit (1) including taken described in connection blood conduit into blood conduit (101) with connect going out for the blood back conduit
Blood conduit (102).
2. the extracorporal circulatory system Cell capture system according to claim 1 for making liquid biopsy, it is characterised in that：It further includes
Blood blood back integrated apparatus (9) is taken, this takes blood blood back integrated apparatus (9) to include the tube body (901) that one end is tip, described to take
Blood conduit and blood back conduit are arranged in the tube body (901) so that formation is mutually independent inside the tube body (901) takes haemocoele
(902) and blood back cavity (903), tube body (901) tip are disposed with the blood back hole (910) for connecting the blood back cavity (903), institute
State tube body (901) side wall be disposed with take haemocoele (902) described in several connections take blood hole (908).
3. the extracorporal circulatory system Cell capture system according to claim 2 for making liquid biopsy, it is characterised in that：It is described to take
Blood hole (908) at least three, each position interlaced arrangement for taking blood hole (908).
4. the extracorporal circulatory system Cell capture system according to claim 3 for making liquid biopsy, it is characterised in that：The pipe
The one end of body (901) far from tip is connected with more siphunculus seats (911), which is connected with described in connection and takes haemocoele
(902) take blood branch pipe (912), which is connected with the blood back branch pipe (913) for connecting the blood back cavity (903).
5. the extracorporal circulatory system Cell capture system according to claim 4 for making liquid biopsy, it is characterised in that：It is described into
For blood conduit (101) by taking blood branch pipe (912) described in the connection of the first female Luer, the bleeding conduit (102) passes through the second Shandong
You connect the blood back branch pipe (913) by connector.
6. the extracorporal circulatory system Cell capture system according to any one of claims 1 to 5 for making liquid biopsy, feature exist
In：The Cell capture device is circulating tumor cell trap setting (4), and the circulating tumor cell trap setting (4) includes
Closed container (401) and the retention unit in the closed container (401), the closed container (401) be disposed with into blood mouth and
Blood outlet, the retention unit include at least one layer of filtering film layer, and each filter membrane layer arrangement has multiple retention micropores.
7. the extracorporal circulatory system Cell capture system according to claim 6 for making liquid biopsy, it is characterised in that：It is each described
The edge of filtering film layer is connect with the inner wall sealing of the closed container (401), is had and each institute in the closed container (401)
State the connecting portion (408) of filtering film layer connection.
8. the extracorporal circulatory system Cell capture system according to claim 7 for making liquid biopsy, it is characterised in that：It is described enter
Blood mouth is connected with the entrance connecting pin (402) of hollow tube-shape, and the blood outlet is connected with the outlet connecting pin of hollow tube-shape
(403)。
9. the extracorporal circulatory system Cell capture system according to claim 6 for making liquid biopsy, it is characterised in that：It is described to follow
Ring tumour cell trap setting (4) is arranged in bleeding conduit (102), and the bleeding conduit (102) is disposed with to be swollen around the cycle
The bypass manifold (103) of oncocyte trap setting (4), the bypass manifold (103) are disposed with easing valve (5), the blood back conduit
(102) it is disposed with the second pressure sensor (6) for controlling easing valve (5) open/close states.
10. the extracorporal circulatory system Cell capture system according to any one of claims 1 to 5 for making liquid biopsy, feature
It is：It is described to be disposed with first pressure sensor (3), the signal of first pressure sensor (3) feedback into blood conduit (101)
Control forward and reverse action of the blood pump (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810107737.1A CN108175448A (en) | 2018-02-02 | 2018-02-02 | A kind of extracorporal circulatory system Cell capture system for making liquid biopsy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810107737.1A CN108175448A (en) | 2018-02-02 | 2018-02-02 | A kind of extracorporal circulatory system Cell capture system for making liquid biopsy |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108175448A true CN108175448A (en) | 2018-06-19 |
Family
ID=62552019
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810107737.1A Pending CN108175448A (en) | 2018-02-02 | 2018-02-02 | A kind of extracorporal circulatory system Cell capture system for making liquid biopsy |
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| CN (1) | CN108175448A (en) |
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| US6177019B1 (en) * | 1996-08-26 | 2001-01-23 | Hemasure Inc. | Method and apparatus for removing tumor cells from tumor cell-contaminated stem cell products |
| JP2002345949A (en) * | 2001-05-30 | 2002-12-03 | Mazda Motor Corp | Cardinal function supporting device |
| JP2004181120A (en) * | 2002-12-06 | 2004-07-02 | Nipro Corp | Multi lumen catheter |
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| CN105979880A (en) * | 2013-12-13 | 2016-09-28 | 直观外科手术操作公司 | Telescoping biopsy needle |
| US20170002308A1 (en) * | 2014-01-14 | 2017-01-05 | The Hunan Cycle Filter Medical Technology Co., Ltd. | Device for separating cells in fluid |
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