CN108210980A - Bioactivity glass microballoon and preparation method thereof - Google Patents
Bioactivity glass microballoon and preparation method thereof Download PDFInfo
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- CN108210980A CN108210980A CN201611195810.2A CN201611195810A CN108210980A CN 108210980 A CN108210980 A CN 108210980A CN 201611195810 A CN201611195810 A CN 201611195810A CN 108210980 A CN108210980 A CN 108210980A
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- glass microballoon
- bioactivity glass
- leaf
- precursor liquid
- radix ophiopogonis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/62—Encapsulated active agents, e.g. emulsified droplets
Abstract
The present invention provides a kind of preparation method of bioactivity glass microballoon, includes the following steps：Take biological template, and by the biological template by activation process, clean, dry, to obtain pore creating material；By nonionic block copolymers stirring and dissolving, and temperature is adjusted, so that micellization occurs for the nonionic block copolymers, to obtain a solution；Triethyl phosphate, ethyl orthosilicate, acidic catalyst, γ glycidyl ether oxygen propyl trimethoxy silicanes and calcium source, stirring and dissolving, to obtain the bioactivity glass microballoon precursor liquid are added in into the solution；The pore creating material is immersed in a period of time in the bioactivity glass microballoon precursor liquid；The product after dipping is taken out, through over cleaning, drying, calcining, to obtain bioactivity glass microballoon.The invention further relates to a kind of bioactivity glass microballoons.The bioactivity glass microballoon good biocompatibility of the present invention, and its preparation process is simple, it is of low cost.
Description
Technical field
The present invention relates to a kind of bioactivity glass microballoons and preparation method thereof.
Background technology
Bioactivity glass is made of to natural bone and tooth similar, has higher bioactivity, biofacies
Capacitive, degradability, no cytotoxicity, and the regeneration of sclerous tissues and soft tissue can be promoted, it is a kind of good bioactivity
Material.
At present, the preparation of bioactivity glass using pollen (such as rape pollen) as biological template, however pollen raw material
Source easily limited by the florescence, the removal step of pollen impurity is cumbersome, and its production cost is high, therefore uses pollen conduct
Biological template prepares bioactivity glass and is not easy industrialized production.
Invention content
In view of the foregoing, it is necessary to provide a kind of of low cost, simple bioactivity glass microballoon of preparation process and
Preparation method.
A kind of method for preparing bioactivity glass microballoon, includes the following steps：
A) take biological template, and by the biological template by activation process, clean, dry, it is described to obtain pore creating material
A kind of or combination of the biological template in liriope platyphylia Wang et Tang leaf, Radix Ophiopogonis leaf, the native Radix Ophiopogonis leaf of broad-leaved, native Radix Ophiopogonis leaf；
B) precursor liquid of bioactivity glass microballoon is prepared, is included the following steps：
By nonionic block copolymers stirring and dissolving, and temperature is adjusted, so that the nonionic block copolymers are sent out
Raw rubber fasciculation, to obtain a solution；
Triethyl phosphate, ethyl orthosilicate, acidic catalyst, γ-glycidyl ether oxygen propyl are added in into the solution
Trimethoxy silane and calcium source, stirring and dissolving, to obtain the bioactivity glass microballoon precursor liquid；
C) pore creating material is immersed in a period of time in the bioactivity glass microballoon precursor liquid；
D) product after dipping is taken out, through over cleaning, drying, calcining, to obtain bioactivity glass microballoon.
The liliaceous plant is selected from liriope platyphylia Wang et Tang, Radix Ophiopogonis, broad-leaved native Radix Ophiopogonis, native Radix Ophiopogonis, evergreen, pink reineckea herb, tawny daylily
In a kind of or combination.
Reagent used in the activation process includes ethanol water, pH adjusting agent and activator.
The pH adjusting agent is acid reagent, and the acid reagent is hydrochloric acid or nitric acid.
A kind of or combination of the activator in methanol, ethylene glycol, polyethylene glycol, glycerine.
The pH of the solution of the activation process is 2-5.
In step a, after the activated processing of biological template, take out the biological template after activation and rinsed with washing lotion,
Until the washing lotion after rinsing is in neutrality.
A kind of or combination of the washing lotion in deionized water, pure water, distilled water or buffer solution.
A concentration of 0.5-15% of the nonionic block copolymers.
The dosage of the pore creating material is 0.5-100g, and the dosage of the bioactivity glass microballoon precursor liquid is 10-
1000g。
The bioactivity glass microballoon has hollow microcellular structure, has connectivity between the micropore.
Compared with the prior art, the preparation method of above-mentioned bioactivity glass microballoon, by using of low cost, and source is rich
The native Radix Ophiopogonis leaf of liriope platyphylia Wang et Tang leaf, Radix Ophiopogonis leaf, soil Radix Ophiopogonis leaf, broad-leaved of richness is as biological template, so as to reduce
Production cost.Further, since liriope platyphylia Wang et Tang leaf, Radix Ophiopogonis leaf, native Radix Ophiopogonis leaf, the native Radix Ophiopogonis leaf of broad-leaved have special gas
Pore structure so that the active constituent of bioactivity glass can effectively, be uniformly dispersed and adsorb in the liliaceous plant leaf
In son, so as to prepare good biocompatibility, specific surface area is larger and good air permeability has liriope platyphylia Wang et Tang leaf
The bioactivity glass microballoon of the microcellular structure of the native Radix Ophiopogonis leaf of son, Radix Ophiopogonis leaf, soil Radix Ophiopogonis leaf, broad-leaved.It is in addition, of the invention
The method for preparing bioactivity glass microballoon is not only simple for process, condition is easily-controllable, and cost is simple, suitable for industrialized production.
Specific embodiment
As used in this article, term " room temperature " has its general sense well known in the art, and description indoor temperature is 25 DEG C
± 5 DEG C degrees Celsius.
The preparation method of bioactivity glass microballoon of the present invention, specifically comprises the following steps：
(1) acquisition and pre-treat biological template.
The biological template uses liliaceous plant blade, and the liliaceous plant is, for example, but is not limited to broad-leaved wheat
Winter, Radix Ophiopogonis, broad-leaved native Radix Ophiopogonis, native Radix Ophiopogonis, evergreen, pink reineckea herb, tawny daylily.It should be understood that the liliaceous plant can be with
Selected from the plant with the similar liriope platyphylia Wang et Tang leaf structure.
The liriope platyphylia Wang et Tang also known as " big Radix Ophiopogonis "；The Radix Ophiopogonis also known as " tuber of dwarf lilyturf ", " dwarf lilyturf "；It is described soil Radix Ophiopogonis also known as
" Radix Ophiopogonis door "；The pink reineckea herb also known as " purple clothing grass "；And the tawny daylily also known as " day lily ", " day lily ", " deer green onion ", " river grass
Flower " etc..
It is the liriope platyphylia Wang et Tang, the Radix Ophiopogonis, the broad-leaved native Radix Ophiopogonis, the native Radix Ophiopogonis, the pink reineckea herb, described evergreen
And the tawny daylily is perennial evergreen herbaceous plant, and it is with wide Regional Distribution.Therefore, the liliaceous plant leaf
Abundant source can reduce production cost.
It should be understood that the gas on the liriope platyphylia Wang et Tang, the Radix Ophiopogonis, the broad-leaved native Radix Ophiopogonis and the native Radix Ophiopogonis blade
Hole moves towards identical in zonal distribution, no accessory cell with blade parallel veins, in two parallelodromous centres.The broad-leaved
Radix Ophiopogonis, the described evergreen and pink reineckea herb have a similar stomatal apparatus structure, such as sunken stomata degree unobvious, stomata are big
Small and similar density.The Radix Ophiopogonis, the native Radix Ophiopogonis, the sunken stomata degree of the broad-leaved native Radix Ophiopogonis and the tawny daylily are brighter
It is aobvious, and stomata is smaller, stomatal frequency is big.Since the size of stomata, distribution and density can influence the bioactivity glass microballoon
The infiltration of contained active constituent and preservation effect, therefore the preferred stomata of liliaceous plant blade is larger, pore opening is poor
Different relatively small and gas cell distribution is smooth, uniform blade.
It should be understood that have compared to the Radix Ophiopogonis, the broad-leaved native Radix Ophiopogonis and the native Radix Ophiopogonis, the liriope platyphylia Wang et Tang
Pore opening difference is relatively small, the upper and lower epidermis of blade all has gas cell distribution, and the stoma number of lower epidermis is far more than upper table
Skin, the epidermal cell percilinal wall at stomatal band make sunken stomata with respect to unobvious substantially without protrusion.Further, since described ten thousand
Young and described pink reineckea herb has the stomatal apparatus structure similar to the liriope platyphylia Wang et Tang.Therefore, the preferred institute of the liliaceous plant
It states from liriope platyphylia Wang et Tang, the described evergreen and pink reineckea herb.
The liriope platyphylia Wang et Tang belongs to monocotyledon, and blade is linear, and vein is parallel train of thought.There is tubular structure in vein
Vascular bundle be interlaced with one another connection through entire blade, form the skeleton of transporting moisture, inorganic salts and organic substance.Blade
Oval stomata is dispersed on epidermis, and longitudinal stomatal band is formed between vein, convenient for the exchange of substance inside and outside leaf.Mesophyll is thin
Born of the same parents' arrangement is loose, and gap is larger, and has big central vacuole in mesophyll cell.The structure feature of the liriope platyphylia Wang et Tang is easy to
Substance gos deep into blade interior and deposits, and then forms the material with hierarchical porous structure.
A) fresh liliaceous plant leaf is taken, the blade that specification is of moderate size is cut into, is cleaned 2-5 times with EtOH Sonicate,
To remove the impurity of leaf surfaces.
It should be understood that those skilled in the art personnel it will be appreciated that, the liliaceous plant leaf can be cut into not
The blade of same specification size, and shape is unrestricted.
Preferably, in order to ensure the liliaceous plant leaf cleaning is more thorough, and during follow-up participation reaction, described in reduction
Reunite between liliaceous plant blade and occur, the liliaceous plant blade cuts into strip shape, and specification size is preferred
For 5mm × 10mm.Therefore, the liliaceous plant blade can fully be reacted in immersion fluid.
B) the liliaceous plant blade after activation process cleaning.
Liliaceous plant blade after cleaning is impregnated in 50% ethanol solution, adds in pH adjusting agent to adjust solution
PH value is about 2-5, and adds in activator activation process 18-48 hours, to remove the gold of the liliaceous plant blade interior
Belong to impurity and part dissolved organic matter.
It should be understood that the activation process of the liliaceous plant blade can make it still keep natural ecology, light
Pool and elasticity are without wilting.
The activator is, for example, but is not limited to, methanol, ethylene glycol, polyethylene glycol or glycerine.
Preferably, the activator is polyethylene glycol.
The pH adjusting agent is acid reagent.
The acid reagent is, for example, but is not limited to, hydrochloric acid or nitric acid.
Preferably, the acid reagent is hydrochloric acid.
Preferably, the pH value of the solution is about 3.
Preferably, the time of the activation process is 24 hours.
C) it takes out the liliaceous plant blade after activation process and is rinsed with washing lotion, until the washing lotion after rinsing is in neutrality,
The liliaceous plant blade is dried again, to obtain pore creating material.
The washing lotion is, for example, but is not limited to, deionized water, pure water, distilled water or buffer solution, to remove described hundred
Remaining acid reagent on conjunction section plant leaf blade.
It should be understood that due to containing micro metal ion, the preferred deionization of washing lotion in the buffer solution
Water, pure water or distilled water.
(2) precursor liquid of bio-vitric activity glass ball is prepared.
A) at room temperature, by 0.01-20g nonionic block copolymers stirring and dissolvings, in 10-500g ethyl alcohol/distilled water
In, and 40-45 DEG C is adjusted the temperature to, micellization occurs for the nonionic block copolymers, to obtain opalescent solution.
It should be understood that the solvent used in the nonionic block copolymers dissolving is in water, distilled water, ethyl alcohol
A kind of or combination.
Preferably, the solvent is the ratio example of ethyl alcohol and the mixed solution of distilled water, the ethyl alcohol and the distilled water
If so, it but is not limited to, 100:0、1:1、2:1 or 0:100.
It should be understood that in order to control the solution concentration after the nonionic block copolymers generation micellization, it is described
The ratio of ethyl alcohol and the distilled water is preferably 1:1.
It should be understood that in micellization generating process, when solution from it is colourless become milky after, stopping heating simultaneously makes institute
The temperature for stating solution is down to room temperature.
The nonionic block copolymers are selected from F127 (molecular formula EO106PO70EO106) or P123, and (molecular formula is
PEO-PPO-PEO)。
Since during the precursor liquid for preparing bioactivity glass microballoon, the calcium of generation product can be reduced using F127
Ion concentration, therefore, the nonionic block copolymers are preferred P123.A concentration of 0.5-15% of the P123.
B) at ambient temperature, by 0.1-10g triethyl phosphates, 1-20g ethyl orthosilicates, 0.5-5g acidic catalysts,
98% γ of 0.1-30g-glycidyl ether oxygen propyl trimethoxy silicane and 0.1-50g calcium sources are added in above-mentioned solution, and stir
16-30 hours, bioactivity glass microballoon precursor liquid is made.
A kind of or combination of the calcium source in calcium oxide, calcium carbonate, calcium nitrate, calcium sulfate, calcium silicates.
Preferably, the calcium source is calcium nitrate.
Preferably, the calcium nitrate is four water-calcium nitrate (molecular formula, Ca (NO3) 24H2O).It should be understood that from life
The surface release calcium ion of object activity glass microballoon helps to form the layer rich in calcium phosphate on the glass surface.
The one kind of the acidic catalyst in hydrochloric acid or hydrofluoric acid.
Preferably, the acidic catalyst is hydrochloric acid.
(3) pore creating material is immersed in the bioactivity glass microballoon precursor liquid；
The pore creating material is added in the bioactivity glass microballoon precursor liquid, and is impregnated at being 10-40 DEG C in temperature
24 hours.The dosage of the pore creating material is 0.5-100g, and the dosage of the bioactivity glass microballoon precursor liquid is 10-1000g,
To realize that reacting between the pore creating material and the bioactivity glass microballoon precursor liquid is smoothed out.
It, not only can be with it should be understood that by the bioactivity glass microballoon precursor liquid for preparing of pore creating material addition
The stomata of the pore creating material is avoided to be blocked, additionally it is possible to the active constituent contained by the bioactivity glass microballoon be made equably to divide
It dissipates and adsorbs in pore creating material.
(4) product after dipping is taken out, successively through over cleaning, drying, calcining, to obtain bioactivity glass microballoon.
The pore creating material being immersed in the bioactivity glass microballoon precursor liquid is taken out, is then cleaned with the EtOH Sonicate
1-5 hours；Product after cleaning is taken out, and it is 48 hours dry at 20-60 DEG C of temperature after, be 450-700 DEG C in temperature
3-8h is calcined under air conditions, to remove template, to obtain bioactivity glass microballoon.
The bioactivity glass microballoon has microcellular structure, and the aperture of the microcellular structure is 1-30nm.The micropore
Structure is hollow structure.In addition, there is connectivity, to improve gas permeability and carry between the micropore of the bioactivity glass microballoon
High-specific surface area, and the outer surface of the bioactivity glass microballoon is more smooth, to reduce bioactivity glass microballoon
Active constituent directly reacts with skin wound, hinders wound person so as to mitigate and is using containing bioactivity glass microballoon
Caused severe pain sense during drug.
It should be understood that since the bioactivity glass microballoon has hollow microcellular structure, and microcellular structure has
Connectivity, so as to which the bioactivity glass microballoon specific surface area is larger, therefore good permeability, reaction speed are fast, can obtain
Quick therapeutic effect.
The preparation method for preparing bioactivity glass microballoon of the present invention, by using the liliaceous plant leaf of abundance
Son is as biological template, so as to reduce production cost.Further, since liliaceous plant leaf has special air hole structure,
Enable bioactivity glass microballoon active constituent effectively, be uniformly dispersed and adsorb in the liliaceous plant leaf
Interior, so as to prepare good biocompatibility, specific surface area is larger and the bioactivity glass microballoon of good air permeability.
It should be understood that the bioactivity glass microballoon is used to prepare wound repair dressing.
The wound repair dressing of the present invention includes bioactivity glass microballoon, aloe extract, the dichloro-benzenes of above-mentioned preparation
Oxygen chlorophenol, medical glycerine, carbomer and triethanolamine, wherein the content of each substance is：The bioactivity glass microballoon
0.5-60 mass parts, the aloe extract 0.001-0.5 mass parts, the triclosan 0.001-0.003 mass parts,
The medical glycerine 1-30 mass parts, the carbomer 0.001-30 mass parts and the triethanolamine 0.001-0.005 matter
Measure part.
The bioactivity glass microballoon is using liliaceous plant leaf as biological template.
It should be understood that the liliaceous plant leaf is selected as biological template can influence the bioactivity glass and is micro-
The microcellular structure that ball is formed.
The liliaceous plant is selected from Radix Ophiopogonis, liriope platyphylia Wang et Tang, native Radix Ophiopogonis, broad-leaved native Radix Ophiopogonis, evergreen, pink reineckea herb, tawny daylily
In a kind of or combination.
Preferably, the liliaceous plant is selected from the liriope platyphylia Wang et Tang, the described evergreen or pink reineckea herb.
The bioactivity glass microballoon has microcellular structure, and the aperture of the micropore is 1-30nm.The bioactivity
The microcellular structure of glass microsphere is hollow nanostructured, and has connectivity between the micropore.
It should be understood that since the bioactivity glass microballoon has hollow microcellular structure, so as to which the biology is living
Property glass microsphere specific surface area is larger, therefore good permeability, reaction speed are fast, can obtain quick therapeutic effect.
The wound repair dressing meta-alkalescence, the pH value of the wound repair dressing is about 6-10.
It should be understood that the catabolite of the bioactivity glass microballoon can promote the generation of growth factor, and promote
Into the procreation of cell, so as to realize the effect for promoting wound healing.Specifically, when the bioactivity glass ball acts on
During wound tissue, the alkali metal ion dissolving contained by bioactivity glass microballoon described first is discharged into ambient body fluid, with body
H in liquid+It exchanges, so as to form silicon-rich layer in material surface, silicon-rich layer absorbs the Ca in ambient body fluid as equiax crystal2+、
PO4 3-And OH-Ion forms apatite layer.The apatite layer originally formed is amorphous, and may then be inhaled in growth process
Receive the CO in tissue3 2-The gradually similar HCA crystal of the ingredient of crystallization adult body bone.In apatite forming process, the biology
The release of contained calcium ion increases the degree of supersaturation in ambient body fluid in activity glass microballoon, and silicon ion is material surface phosphorus
The formation of lime stone provides advantageous nucleation site.Therefore, the bioactivity glass microballoon and the alkaline environment at solution interface
Be conducive to the precipitation and crystallization of carbonated hydroxyapatite (Hydroxy-carbonate-apatite, abbreviation HCA) phase.In addition, institute
HCA is stated with the composition and composition very much like with natural bone inorganic constituents, and with good biocompatibility, so as to make
Obtain the quick reparation that the HCA may advantageously facilitate the biological tissue that is wound.Therefore, in order to neutralize be wound wound location generation acidity
Substance and ensure to form carbonated hydroxyapatite (HCA), the pH value of the wound repair dressing is preferably 8.
The wound repair dressing is gelling agent or applicator.
The aloe extract contains control cell growth and the ingredient broken up, moisturizing ingredient, anti-infective ingredient, stimulation in vain
The ingredient of cell and other immune cell growths, the ingredient for promoting callus.For example, the aloe extract contains various active
Enzyme.The organized enzyme includes, but are not limited to, and catalase, amylase, oxidizing ferment, cellulase, lipase, plant coagulate
Sanguinin.The lectins can be attached on human body cell, natural growth factor in human activin cell, stimulate cell interior
Reaction promotes cell growth and division, accelerates wounded tissue rehabilitation and healing, enhances lymphocyte function, so as to improve human body
The ability of various diseases is resisted, improves human body anti-infection ability.
It should be understood that when skin injury, the evaporation of moisture of skin easily accumulates salinity, therefore internal moisture in wound
It can largely be siphoned away by salt, so that the cell mortality of wound surface, so as to cause wound pain.Therefore, it is applied in the surface of a wound
The aloe extract with moisture-keeping function is added in material, the skin wound that can be avoided exposure in air is precipitated salinity and occurs
It is dry and cracked, so as to mitigate wound pain.
In addition, the aloe extract has good biocompatibility, skin compatibility, film forming, gas permeability, moisturizing
Property.Since the aloe extract can not only improve human body anti-infection ability, moreover it is possible to promote wound healing, and scar can be prevented
The formation of trace, therefore the aloe extract and bioactivity glass microballoon can be formed into curative effect under certain mass ratio
Apparent dressing.
The glycerine has plasticising and low-temperature protection effect, so as to improve the mechanical property of above-mentioned dressing and surface topography.
The triclosan also known as " triclosan ", " triclosan ", " Triclosan " etc..The triclosan is one
Kind broad spectrum antimicrobial agent, to causing infection or pathogenicity Gram positive mattress, true mattress, yeast and virus (such as first, hepatitis B, rabies
Poison, AIDS virus HIV) etc. all there is extensive kill and inhibiting effect.Therefore, the surface of a wound of animal body is not easy bacterial infection or disease
Poison is conducive to quickly repair the tissue that is wound.
In the present embodiment, the carbomer is Acritamer 940 GE, wherein, GE represents pharmaceutical grade.
It should be understood that the carbomer is poly- alkyl sucrose or poly- alkyl pentaerythrite and acrylic crosslinking polymer
Copolymer.Due to containing acid groups in the carbomer molecular structure, so as to have certain acidity, therefore the wound can be neutralized
The partial alkaline substance in dressing is repaired in face, to reduce stimulation of the wound repair dressing to skin.The carbomer is through institute
After stating alkaline matter neutralization, contained carboxylic ions in the carbomer molecular structure, due to the mutually exclusive work of negative electrical charge
With so that strand disperse is stretched, and in great swelling state, and has viscosity, so as to form gel.In addition, the carbomer
With stronger hygroscopicity, therefore it can absorb the body fluid that wound location generates that is wound, and promote the body fluid and institute
It states component contained in bioactivity glass microballoon and plysiochemical reaction occurs.
The triethanolamine is in alkalinity, and the triethanolamine is for neutralizing carbomer, to form stable macromolecular structure,
Reach the application effect of thickening and moisturizing.The triethanolamine is soluble in the medical glycerine.
It will be appreciated by those skilled in the art that the solvent of the wound repair dressing is not limited to the medical glycerine, described
Carbomer and the triethanolamine, other can realize that the liquid of the wound repair dressing composition dissolving can be used for this hair
It is bright.
The method of the wound repair dressing, includes the following steps in the preparation of the present invention：
A), raw material is weighed by following weight proportions：The bioactivity glass microballoon 0.5-60 mass parts, the aloe carry
Take object 0.001-0.5 mass parts, the triclosan 0.001-0.003 mass parts, the medical glycerine 1-30 mass parts,
The carbomer 0.001-30 mass parts and the triethanolamine 0.001-0.005 mass parts.
It should be understood that the carbomer can be mixed first with the triethanolamine, to avoid in the carbomer and institute
Bioactivity glass microballoon is stated, so that it is guaranteed that physiology occurs is anti-for the active constituent of the bioactivity glass microballoon and wound tissue
Alkaline environment can be directly provided at once.
B) raw material, is mixed, at a temperature of 80-110, stirring, vacuum defoaming are made described living containing biology into gel
The dressing of property glass microsphere.
The present invention wound repair dressing preparation method by the way that each component is mixed, and under preference temperature carry out into
The processing such as type, drying, you can obtain product, preparation method is simple for process, and condition is easily-controllable, and of low cost, suitable for making extensively
With.
The wound repair dressing of the microballoon containing bioactivity glass of the present invention is used as soft tissue wound repair materials, is suitable for
Burn, cut wound, surgical wound surface, cervical erosion, bedsore, sinus, liquefaction of fat wound, diabetes and all kinds of skin surfaces are burst
The chronic large area surface of a wound such as ulcer.
The present invention is specifically described below by embodiment.
In the present embodiment, the biological template uses liriope platyphylia Wang et Tang leaf and evergreen leaf, and the pH adjusting agent is
Hydrochloric acid, the nonionic block copolymers are P123.The activator is polyethylene glycol.The washing lotion is pure water.It is described
Calcium source is four water-calcium nitrate.
Embodiment 1
(1) pretreatment of liriope platyphylia Wang et Tang leaf：
Fresh liriope platyphylia Wang et Tang leaf is chosen, is cut into the blade of 5mm × 10mm sizes, is cleaned 3 times with EtOH Sonicate.Then
Liriope platyphylia Wang et Tang blade after cleaning is placed in 50% ethanol water, the pH value for adding in hydrochloric acid conditioning solution is about 3, and is added
Enter polyethylene glycol activation process for 24 hours.The liriope platyphylia Wang et Tang blade after activation process is taken out, and is rinsed with pure water, until after rinsing
Washing lotion be in neutrality, then dry the liriope platyphylia Wang et Tang blade, be made pore creating material.
(2) precursor liquid of bioactivity glass microballoon is prepared.
At room temperature, by 5g P123 stirring and dissolvings in 15g ethyl alcohol/distilled water (ratio 1:1) it in, and adjusts the temperature to
45 DEG C, micellization occurs for the P123, to obtain opalescent solution.
When above-mentioned solution is down to room temperature, by 1g triethyl phosphates, 8g ethyl orthosilicates, 1g acidic catalysts, 2g 98%
γ-glycidyl ether oxygen propyl trimethoxy silicane and 2g four water-calcium nitrates are added in above-mentioned solution, and are stirred 24 hours, with system
Obtain bioactivity glass microballoon precursor liquid.
(3) bioactivity glass microballoon is prepared.
It weighs pore creating material described in 20g to add in bioactivity glass microballoon precursor liquid described in 500g, and is 40 DEG C in temperature
It is lower to impregnate 24 hours；Product after impregnating is then taken out, is cleaned 2 hours with EtOH Sonicate；By the product after cleaning in temperature 50 C
After lower drying 48 hours, 3h is calcined in the case where temperature is 700 DEG C of air conditions, to remove template, to obtain bioactivity glass
Microballoon.
(4) wound repair dressing is prepared.
Raw material is weighed by following weight proportions：10 mass parts of bioactivity glass microballoon of the preparation of embodiment 1, the aloe
20 mass parts of extract, 0.001 mass parts of the triclosan, 10 mass parts of the medical glycerine, 5 matter of the carbomer
Measure 0.002 mass parts of part and the triethanolamine.
The raw material is mixed, at a temperature of 80 DEG C, stirring, vacuum defoaming are made described and contain bioactivity glass into gel
The wound repair dressing of glass microballoon.
Embodiment 2
(1) pretreatment of evergreen leaf：
Fresh evergreen leaf is chosen, is cut into the blade of 5mm × 10mm sizes, is cleaned 3 times with EtOH Sonicate.Then will
Evergreen blade after cleaning is placed in 50% ethanol water, and the pH value for adding in hydrochloric acid conditioning solution is about 3, and is added in poly-
Ethylene glycol activation process is for 24 hours.The evergreen blade after activation process is taken out, and is rinsed with pure water, until the washing lotion after rinsing
It is in neutrality, then dries the evergreen blade, pore creating material is made.
(2) precursor liquid of bioactivity glass microballoon is prepared.
At room temperature, by 5g P123 stirring and dissolvings in 15g ethyl alcohol/distilled water (ratio 1:1) it in, and adjusts the temperature to
45 DEG C, micellization occurs for the P123, to obtain opalescent solution.
When above-mentioned solution is down to room temperature, by 1g triethyl phosphates, 8g ethyl orthosilicates, 1g acidic catalysts, 2g 98%
γ-glycidyl ether oxygen propyl trimethoxy silicane and 2g four water-calcium nitrates are added in above-mentioned solution, and are stirred 24 hours, with system
Obtain bioactivity glass microballoon precursor liquid.
(3) bioactivity glass microballoon is prepared.
It weighs pore creating material described in 20g to add in bioactivity glass microballoon precursor liquid described in 500g, and is 40 DEG C in temperature
It is lower to impregnate 24 hours；Product after impregnating is then taken out, is cleaned 2 hours with EtOH Sonicate；By the product after cleaning in temperature 50 C
After lower drying 48 hours, 3h is calcined in the case where temperature is 700 DEG C of air conditions, to remove template, to obtain bioactivity glass
Microballoon.
(4) wound repair dressing is prepared.
Raw material is weighed by following weight proportions：10 mass parts of bioactivity glass microballoon of the preparation of embodiment 2, the aloe
20 mass parts of extract, 0.001 mass parts of the triclosan, 10 mass parts of the medical glycerine, 5 matter of the carbomer
Measure 0.002 mass parts of part and the triethanolamine.
The raw material is mixed, at a temperature of 80 DEG C, stirring, vacuum defoaming are made described and contain bioactivity glass into gel
The wound repair dressing of glass microballoon.
The preparation method of the bioactivity glass microballoon of the present invention, the leaf by using liliaceous plant are biological mould
Plate, by using of low cost, and the liliaceous plant leaf of abundance is produced into as biological template so as to reduce
This.Further, since liliaceous plant leaf has special air hole structure so that the active constituent energy of bioactivity glass microballoon
Enough effectively, it is uniformly dispersed and adsorbs in the liliaceous plant leaf, so as to prepare good biocompatibility, compare table
Area is larger and the bioactivity glass microballoon of good air permeability.The wound of preparation microballoon containing bioactivity glass of the present invention
Face repair dressing method is not only simple for process, condition is easily-controllable, and cost is simple, suitable for industrialized production.The surface of a wound of the present invention
It repairs dressing and includes bioactivity glass microballoon and aloe extract, since there is the bioactivity glass microballoon special hole to tie
Structure, bioactivity glass microballoon has good biocompatibility, and the aloe extract can not only improve the anti-sense of human body
Dye ability, moreover it is possible to promote wound healing, and the formation of scar can be prevented.Therefore, the wound repair dressing can be treated quickly
The wound of the wounded, moreover it is possible to mitigate the feeling of pain of the wounded over the course for the treatment of.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, embodiment of above are only for interpreting the claims.Right protection scope of the present invention is not limited to specification.Appoint
What those familiar with the art is in the technical scope of present disclosure, the variation or replacement that can readily occur in,
It is included within protection scope of the present invention.
Claims (10)
1. a kind of method for preparing bioactivity glass microballoon, includes the following steps：
A) take biological template, and by the biological template by activation process, clean, dry, to obtain pore creating material, the biology
A kind of or combination of the template in liriope platyphylia Wang et Tang leaf, Radix Ophiopogonis leaf, the native Radix Ophiopogonis leaf of broad-leaved, native Radix Ophiopogonis leaf；
B) precursor liquid of bioactivity glass microballoon is prepared, is included the following steps：
By nonionic block copolymers stirring and dissolving, and temperature is adjusted, so that glue occurs for the nonionic block copolymers
Fasciculation, to obtain a solution；
Triethyl phosphate, ethyl orthosilicate, acidic catalyst, γ-glycidyl ether oxygen propyl front three are added in into the solution
Oxysilane and calcium source, stirring and dissolving, to obtain the bioactivity glass microballoon precursor liquid；
C) pore creating material is immersed in a period of time in the bioactivity glass microballoon precursor liquid；
D) product after dipping is taken out, through over cleaning, drying, calcining, to obtain bioactivity glass microballoon.
2. the method as described in claim 1, it is characterised in that：Reagent used in the activation process includes ethanol solution, pH
Conditioning agent and activator.
3. method as claimed in claim 2, it is characterised in that：The pH adjusting agent is acid reagent, and the acid reagent is
Hydrochloric acid or nitric acid.
4. method as claimed in claim 2, it is characterised in that：The activator is selected from methanol, ethylene glycol, polyethylene glycol, sweet
A kind of or combination in oil.
5. the method as described in claim 1, it is characterised in that：The pH of the solution of the activation process is 2-5.
6. the method as described in claim 1, it is characterised in that：In step a, after the activated processing of biological template, take
Go out activation after biological template and rinsed with washing lotion, until rinse after washing lotion be in neutrality.
7. method as claimed in claim 6, it is characterised in that：The washing lotion is selected from deionized water, pure water, distilled water or slow
A kind of or combination in fliud flushing.
8. the method as described in claim 1, it is characterised in that：A concentration of 0.5- of the nonionic block copolymers
15%.
9. the method as described in claim 1, it is characterised in that：The dosage of the pore creating material is 0.5-100g, and the biology is living
Property glass microsphere precursor liquid dosage be 10-1000g.
10. a kind of bioactivity glass microballoon prepared using method described in claim 1, it is characterised in that：The biology
Activity glass microballoon has hollow microcellular structure, has connectivity between the micropore.
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| CN102068706A (en) * | 2010-12-30 | 2011-05-25 | 上海师范大学 | Preparation method of silicon dioxide (SiO2) ultrasonic imaging radiography material |
| CN103463684A (en) * | 2013-06-04 | 2013-12-25 | 吉林大学 | Bone restoration material treating rapid filter paper as template, and preparation method and application thereof |
| CN103463677A (en) * | 2013-06-04 | 2013-12-25 | 吉林大学 | Magnetic hierarchically-porous nanometer sustained-release bone restoration material, and preparation method and application thereof |
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Application publication date: 20180629 |