CN108210727A - A kind of yishenbugu liquid and preparation method thereof - Google Patents

A kind of yishenbugu liquid and preparation method thereof Download PDF

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Publication number
CN108210727A
CN108210727A CN201810220941.4A CN201810220941A CN108210727A CN 108210727 A CN108210727 A CN 108210727A CN 201810220941 A CN201810220941 A CN 201810220941A CN 108210727 A CN108210727 A CN 108210727A
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liquid
yishenbugu
filtration
parts
preparation
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曾胜
曾佐达
梁烽焱
张�浩
潘洁丽
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GUANGDONG YILI GROUP PHARMACEUTICAL CO Ltd
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GUANGDONG YILI GROUP PHARMACEUTICAL CO Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8969—Polygonatum (Solomon's seal)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/26—Iron; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/076—Poria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/11—Pteridophyta or Filicophyta (ferns)
    • A61K36/12—Filicopsida or Pteridopsida
    • A61K36/126—Drynaria
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/232—Angelica
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/34—Campanulaceae (Bellflower family)
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
    • A61K36/704—Polygonum, e.g. knotweed
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/752—Citrus, e.g. lime, orange or lemon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/80—Scrophulariaceae (Figwort family)
    • A61K36/804—Rehmannia
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K36/18—Magnoliophyta (angiosperms)
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    • A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • A61K36/815—Lycium (desert-thorn)
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/46—Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
    • A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

Abstract

The invention discloses a kind of yishenbugu liquid and preparation method thereof.The preparation method takes the medicinal materials such as the rhizome of davallia, the fleece-flower root, Poria cocos, teasel root including S1, adds water to cook 2 times, filtration, merging filtrate, and after concentration plus 2 times of amounts of ethyl alcohol, stirring are stood, and filtration recycles ethyl alcohol, obtains mixed extract；S2 Radix Paeoniae Albas decoct 2 times after adding water, and filtration, merging filtrate is concentrated to give concentrate；S3 concentrates add 1 times of amount of water, add egg white solution, are heated to boiling, let cool, filter, and adjust pH value to 3.5~3.8, stand, filtration obtains white paeony root extract；S4 mixes mixed extract and white paeony root extract, adds sucrose, white wine and preservative, adjusts pH value to 4.0~4.4, and filtration adds water constant volume, stir evenly, embedding, sterilizing to get.The present invention solve thes problems, such as that existing yishenbugu liquid precipitates soon after heat sterilization, obtains the yishenbugu liquid placed and have no that precipitation generates and curative effect is constant for 2 years or more.

Description

A kind of yishenbugu liquid and preparation method thereof
Technical field
The invention belongs to technical field of traditional Chinese medicines, and in particular to a kind of yishenbugu liquid and preparation method thereof.
Technical background
The yishenbugu Ye Xi Ministry of Public Health issues the kind that the drug standards (Traditional Chinese medicine historical preparation the 10th) are recorded, and standard number is WS3- B-2020-95 has the effect of nourishing liver and kidney, strong muscles and bones, for kidney deficiency and liver, internal lesion caused by overexertion pain in the back, injured bone.At it Side：Rhizome of davallia 45g, fleece-flower root 126g, Poria cocos 63g, teasel root 63g, Radix Paeoniae Alba 44g, Radix Angelicae Sinensis 63g, Radix Codonopsis 75g, prepared rehmannia root 63g, Huang Smart 63g, fruit of Chinese wolfberry 63g, native copper (forge, vinegar is quenched) 45g and dried orange peel 16g.Preparation method：More than 12 taste Chinese medicines, add water to cook two Secondary, 2 hours every time, filtration, merging filtrate was concentrated into every 1ml solution and is equivalent to crude drug 1g, added 2 times of amounts of ethyl alcohol, and stirring is quiet It puts, filters, recycle ethyl alcohol, be concentrated into appropriate.Another to add in sucrose 167g, white wine 20ml, Preservative qs, after dissolving, filtration adds Water adjusts total liquid measure to 1000ml, stir evenly to get.
Yishenbugu liquid is the supernatant liquid of sepia, and gas is fragrant, sweet, micro-pungent.But the benefit prepared according to pharmacopeia technique The precipitation of more amount can occur successively soon after heat sterilization in kidney Psoralen liquid, though these precipitations do not affect the treatment, and it is serious Product appearance is influenced, the undesirable impression of product quality is caused to consumer, so as to be unfavorable for that consumer is stimulated to buy.However now The problem of being precipitated for yishenbugu liquid is but without good solution.
PH value is related to liquid preparation stability.Liquid alkalinity is stronger, and alkaloid is easily precipitated, when acid stronger, part soap The easy Precipitation of glycosides, therefore, the clarity of traditional Chinese medicine oral liquid are influenced significantly by pH value.As tendril-leaved fritillary bulb liquid extract adjusts pH value to 7 left sides The right side makes insoluble matter be removed after being precipitated, is added in the mixed liquor of 5.56 other compositions of pH value, and makes the pH value of final products be 6.0 or so, Fel Serpentis et Bulbus Fritillariae Cirrhosae Liquidus clarity can be improved.The clarity of compound rubarb oral liquid becomes with the variation of solution ph Change, acidity is bigger, stability is smaller, but when pH value is more than 7.5 generates a large amount of flocculent deposits, steady when pH value is transferred to 7.0 or so It is qualitative best.As it can be seen that traditional Chinese medicine oral liquid has its respective optimum pH, and not yet probe into out the most suitable of yishenbugu liquid at present PH value.
To sum up, it is badly in need of seeking a kind of method to solve the problems, such as that traditional yishenbugu liquid precipitates after depositing long.
Invention content
In order to solve the problems, such as that precipitation can occur successively soon after heat sterilization in yishenbugu liquid prepared by pharmacopeia technique, The present invention provides a kind of preparation method of yishenbugu liquid, yishenbugu liquid made from this method is placed 2 years or more and has no heavy It forms sediment and generates.
The purpose of the present invention will be further described in detail below reflect and description.
A kind of preparation method of yishenbugu liquid provided by the invention, wherein, the Chinese medicine preparation packet of the yishenbugu liquid Include the medicinal material of following parts by weight meter：45 parts of the rhizome of davallia, 126 parts of the fleece-flower root, 63 parts of Poria cocos, 63 parts of teasel root, 44 parts of Radix Paeoniae Alba, Radix Angelicae Sinensis 63 16 parts of part, 75 parts of Radix Codonopsis, 63 parts of prepared rehmannia root, 63 parts of sealwort, 63 parts of the fruit of Chinese wolfberry, 45 parts of native copper (forging, vinegar is quenched) and dried orange peel；
The preparation method specifically includes following steps：
S1, the rhizome of davallia for taking formula ratio, the fleece-flower root, Poria cocos, teasel root, Radix Angelicae Sinensis, Radix Codonopsis, prepared rehmannia root, sealwort, the fruit of Chinese wolfberry, from Right copper and dried orange peel add in the water of medicinal material 4~6 times of weight of total weight, decoct 2 hours, and filtration, filter residue adds in medicinal material total weight 2~3 The water of times weight decocts 2 hours, and filtration, merging filtrate is concentrated into every 1ml solution and is equivalent to crude drug 1g, adds 2 times of bodies of ethyl alcohol Accumulated amount, stirring, stands 16h at 5~10 DEG C, filters, and recycles ethyl alcohol, obtains mixing herbal extract；
S2, the Radix Paeoniae Alba for taking formula ratio add in the water of Radix Paeoniae Alba 2~4 times of weight of weight, decoct 2 hours, and filtration, filter residue adds in The water of 2~3 times of weight of Radix Paeoniae Alba weight decocts 2 hours, and filtration, merging filtrate is concentrated into every 1ml solution and is equivalent to crude drug 1g, Obtain concentrate；
S3, concentrate is taken, after adding in 1 times of volume dilution of water, obtains dilution, add in egg white solution, stir evenly, slowly add Heat is let cool to boiling and being kept for 3~5 minutes, is filtered, and is adjusted pH value to 3.5~3.8, is stood, filtration obtains white paeony root extract；
S4, herbal extract and white paeony root extract mixing will be mixed, and will add in 167 parts of sucrose, 20 parts of white wine and preservative, stir After mixing dissolving, adjust pH value to 4.0~4.4, filtration adds water constant volume, stir evenly, embedding, sterilizing to get.
Further, the concentration of volume percent of the ethyl alcohol is 90~95%.
Further, the addition volume of the egg white solution is the 2~4% of dilution volume.
Further, the preservative includes 0.5 part of 2.5 parts of benzoic acid and ethyl hydroxy benzoate.
Further, in the preparation method, pH value is adjusted using citric acid-sodium citrate buffer.
The generation precipitated in traditional Chinese medicine oral liquid is since certain ingredients in Chinese medicine form unstable glue in most cases Caused by body particle, during sterilizing, these are tiny, are difficult to colloidal particles are removed by filtration and are aggregated into larger particles so as to accelerate Sedimentation.And inventor in experiments it is found that, the generation of this precipitation is mainly caused by Radix Paeoniae Alba.Therefore, in preparation process of the present invention It is individually operated to Radix Paeoniae Alba progress, while liquid is made to keep clear and bright to adsorb colloidal solid using egg white solution as fining agent.From efficient Liquid chromatography results see that radix paeoniae alba extraction still remains the principle active component Chinese herbaceous peony in crude drug well after egg white is clarified Medicine glycosides.The acidity of white paeony root extract is stronger, pH value usually 2 hereinafter, and often led to after heat sterilization the pH value of finished product into One step declines, and under relatively low pH value condition, certain ingredients easily generate Precipitation in oral liquid, therefore individually by its pH value tune It is whole to 3.5~3.8, after then white paeony root extract is mixed with mixing herbal extract, then pH value adjusted to 4.0~4.4, had Effect solves the problems, such as that traditional yishenbugu liquid precipitates successively soon after heat sterilization.
Correspondingly, the present invention also provides yishenbugu liquid, the benefits made from the preparation method as above-mentioned yishenbugu liquid Kidney Psoralen liquid still remains the effect of original, and system pH is 4.0~4.4, and good stability, it is heavy that placement there are no for 2 years or more It forms sediment and generates.
Therefore, compared with prior art, the present invention has following advantage：
(1) present invention preparation process of existing yishenbugu liquid is improved, by this taste medicinal material of Radix Paeoniae Alba into Row individually extraction, gained radix paeoniae alba extraction are successively adjusted by egg white clarifying treatment, pH value, then are mixed with remaining herbal extract It closes, then adjusts pH value again after other auxiliary materials are added, the yishenbugu liquid for efficiently solving traditional handicraft preparation is heating The problem of being precipitated successively soon after sterilizing obtains the kidney-nourishing benefit placed and have no that precipitation generates and curative effect is constant for 2 years or more Bone liquid.
(2) preparation method of yishenbugu liquid of the present invention is simple, and condition is controllable, and process stabilizing is produced on a large scale, and can push away Wide application.
Specific embodiment
Below by specific embodiment, the present invention is described in further detail.
Embodiment 1, yishenbugu liquid of the present invention and its preparation
Chinese medicine preparation：Rhizome of davallia 45g, fleece-flower root 126g, Poria cocos 63g, teasel root 63g, Radix Paeoniae Alba 44g, Radix Angelicae Sinensis 63g, Radix Codonopsis 75g, prepared rehmannia root 63g, sealwort 63g, fruit of Chinese wolfberry 63g, native copper (forge, vinegar is quenched) 45g and dried orange peel 16g；
Preparation method：
S1, the rhizome of davallia for taking formula ratio, the fleece-flower root, Poria cocos, teasel root, Radix Angelicae Sinensis, Radix Codonopsis, prepared rehmannia root, sealwort, the fruit of Chinese wolfberry, from Right copper and dried orange peel, add in 2.74kg water, decoct 2 hours, and filtration, filter residue adds in 1.37kg water, decoct 2 hours, and filtration merges filter Liquid is concentrated into every 1ml solution and is equivalent to crude drug 1g (i.e. 685ml), adds 2 times of volumes (i.e. 1370ml) of ethyl alcohol, stirring, in 5 16h is stood at DEG C, is filtered, recycles ethyl alcohol, obtains mixing herbal extract；
S2, the Radix Paeoniae Alba for taking formula ratio add in 88g water, decoct 2 hours, and filtration, filter residue adds in 88g water, decoct 2 hours, filter It crosses, merging filtrate, is concentrated into every 1ml solution and is equivalent to crude drug 1g (i.e. 44ml), obtain concentrate；
S3, concentrate is taken, after adding in 1 times of volume (i.e. 44ml) dilution of water, obtains dilution 88ml, add in 1.76ml egg white Liquid stirs evenly, and is slowly heated to boil and be kept for 3 minutes, is let cool, filtration, adjusts pH value to 3.5, stands, filtration obtains White paeony root extract；
S4, mixing herbal extract and white paeony root extract are mixed, add in sucrose 167g, white wine 20g, benzoic acid 2.5g and Ethyl hydroxy benzoate 0.5g after stirring and dissolving, adjusts pH value to 4.0, and filtration adds water to be settled to 1000ml, stir evenly, embedding, sterilizing, i.e., .
The concentration of volume percent of the ethyl alcohol is 90%；PH value is adjusted using citric acid-sodium citrate buffer.
The dilution and white paeony root extract in step S3 are taken, measures containing for Paeoniflorin respectively using high performance liquid chromatography Amount, as a result：The content of Paeoniflorin is 1.802mg/g in dilution, and the content of Paeoniflorin is 1.712mg/g in white paeony root extract.
Embodiment 2, yishenbugu liquid of the present invention and its preparation
Chinese medicine preparation：Rhizome of davallia 45g, fleece-flower root 126g, Poria cocos 63g, teasel root 63g, Radix Paeoniae Alba 44g, Radix Angelicae Sinensis 63g, Radix Codonopsis 75g, prepared rehmannia root 63g, sealwort 63g, fruit of Chinese wolfberry 63g, native copper (forge, vinegar is quenched) 45g and dried orange peel 16g；
Preparation method：
S1, the rhizome of davallia for taking formula ratio, the fleece-flower root, Poria cocos, teasel root, Radix Angelicae Sinensis, Radix Codonopsis, prepared rehmannia root, sealwort, the fruit of Chinese wolfberry, from Right copper and dried orange peel, add in 4.11kg water, decoct 2 hours, and filtration, filter residue adds in 2.055kg water, decoct 2 hours, filter, and merge Filtrate is concentrated into every 1ml solution and is equivalent to crude drug 1g (i.e. 685ml), adds 2 times of volumes (i.e. 1370ml) of ethyl alcohol, stirring, in 16h is stood at 10 DEG C, is filtered, recycles ethyl alcohol, obtains mixing herbal extract；
S2, the Radix Paeoniae Alba for taking formula ratio add in 176g water, decoct 2 hours, and filtration, filter residue adds in 132g water, decoct 2 hours, Filtration, merging filtrate are concentrated into every 1ml solution and are equivalent to crude drug 1g (i.e. 44ml), obtain concentrate；
S3, concentrate is taken, after adding in 1 times of volume (i.e. 44ml) dilution of water, obtains dilution 88ml, add in 3.52ml egg white Liquid stirs evenly, and is slowly heated to boil and be kept for 5 minutes, is let cool, filtration, adjusts pH value to 3.8, stands, filtration obtains White paeony root extract；
S4, mixing herbal extract and white paeony root extract are mixed, add in sucrose 167g, white wine 20g, benzoic acid 2.5g and Ethyl hydroxy benzoate 0.5g after stirring and dissolving, adjusts pH value to 4.4, and filtration adds water to be settled to 1000ml, stir evenly, embedding, sterilizing, i.e., .
The concentration of volume percent of the ethyl alcohol is 95%；PH value is adjusted using citric acid-sodium citrate buffer.
The dilution and white paeony root extract in step S3 are taken, measures containing for Paeoniflorin respectively using high performance liquid chromatography Amount, as a result：The content of Paeoniflorin is 1.891mg/g in dilution, and the content of Paeoniflorin is 1.753mg/g in white paeony root extract.
Embodiment 3, yishenbugu liquid of the present invention and its preparation
Chinese medicine preparation：Rhizome of davallia 45g, fleece-flower root 126g, Poria cocos 63g, teasel root 63g, Radix Paeoniae Alba 44g, Radix Angelicae Sinensis 63g, Radix Codonopsis 75g, prepared rehmannia root 63g, sealwort 63g, fruit of Chinese wolfberry 63g, native copper (forge, vinegar is quenched) 45g and dried orange peel 16g；
Preparation method：
S1, the rhizome of davallia for taking formula ratio, the fleece-flower root, Poria cocos, teasel root, Radix Angelicae Sinensis, Radix Codonopsis, prepared rehmannia root, sealwort, the fruit of Chinese wolfberry, from Right copper and dried orange peel, add in 3.425kg water, decoct 2 hours, and filtration, filter residue adds in 2.055kg water, decoct 2 hours, filter, and merge Filtrate is concentrated into every 1ml solution and is equivalent to crude drug 1g (i.e. 685ml), adds 2 times of volumes (i.e. 1370ml) of ethyl alcohol, stirring, in 16h is stood at 6 DEG C, is filtered, recycles ethyl alcohol, obtains mixing herbal extract；
S2, the Radix Paeoniae Alba for taking formula ratio add in 132g water, decoct 2 hours, and filtration, filter residue adds in 88g water, decoct 2 hours, Filtration, merging filtrate are concentrated into every 1ml solution and are equivalent to crude drug 1g (i.e. 44ml), obtain concentrate；
S3, concentrate is taken, after adding in 1 times of volume (i.e. 44ml) dilution of water, obtains dilution 88ml, add in 2.64ml egg white Liquid stirs evenly, and is slowly heated to boil and be kept for 4 minutes, is let cool, filtration, adjusts pH value to 3.6, stands, filtration obtains White paeony root extract；
S4, mixing herbal extract and white paeony root extract are mixed, add in sucrose 167g, white wine 20g, benzoic acid 2.5g and Ethyl hydroxy benzoate 0.5g after stirring and dissolving, adjusts pH value to 4.2, and filtration adds water to be settled to 1000ml, stir evenly, embedding, sterilizing, i.e., .
The concentration of volume percent of the ethyl alcohol is 90%；PH value is adjusted using citric acid-sodium citrate buffer.
The dilution and white paeony root extract in step S3 are taken, measures containing for Paeoniflorin respectively using high performance liquid chromatography Amount, as a result：The content of Paeoniflorin is 1.863mg/g in dilution, and the content of Paeoniflorin is 1.821mg/g in white paeony root extract.
Comparative example 1
Yishenbugu liquid is prepared according to pharmacopeia technique, specially：12 taste Chinese medicines of formula ratio are taken, add in 3.645kg Water decocts 2 hours, and filtration, filter residue adds in 2.187kg water, decocts 2 hours, and filtration, merging filtrate is concentrated into every 1ml solution phase It when in crude drug 1g (i.e. 729ml), adding 2 times of volumes (i.e. 1458ml) of ethyl alcohol, stirs, 16h is stood at 6 DEG C, filter, recycling Ethyl alcohol；Add in sucrose 167g, white wine 20g, benzoic acid 2.5g and ethyl hydroxy benzoate 0.5g, after stirring and dissolving, filtration adds water to be settled to 1000ml, stir evenly, embedding, sterilizing to get.The concentration of volume percent of the ethyl alcohol is 90%.
Comparative example 2
Compared with Example 3, this comparative example differs only in：The egg white solution of 2.64ml is substituted using the chitosan of 1.0g As fining agent.
The dilution and white paeony root extract in step S3 are taken, measures containing for Paeoniflorin respectively using high performance liquid chromatography Amount, as a result：The content of Paeoniflorin is 1.869mg/g in dilution, and the content of Paeoniflorin is 1.012mg/g in white paeony root extract. As it can be seen that compared to chitosan, using egg white as the fining agent of white paeony root extract, to the retention rate of active ingredient Paeoniflorin more Height, clarifying effect are more preferable.
Comparative example 3
Compared with Example 3, this comparative example differs only in：The egg white solution that 2.64ml is substituted using the gelatin of 1.0g is made For fining agent.
The dilution and white paeony root extract in step S3 are taken, measures containing for Paeoniflorin respectively using high performance liquid chromatography Amount, as a result：The content of Paeoniflorin is 1.865mg/g in dilution, and the content of Paeoniflorin is 0.517mg/g in white paeony root extract. As it can be seen that compared to gelatin, using egg white as the fining agent of white paeony root extract, to the retention rate higher of active ingredient Paeoniflorin, Clarifying effect is more preferable.
Comparative example 4
Compared with Example 3, this comparative example differs only in：In step S4, pH value is adjusted to 5.0.
Comparative example 5
Compared with Example 3, this comparative example differs only in：In step S4, pH value is adjusted to 3.5.
The quality inspection of test example one, yishenbugu liquid of the present invention
Quality inspection is carried out to the yishenbugu liquid of the embodiment of the present invention 1~3, as a result such as the following table 1：
The quality test results of 1 yishenbugu liquid of the present invention of table
To sum up, yishenbugu liquid of the present invention presses the tenth WS of the Sanitation Ministry medicine standard Traditional Chinese medicine historical preparation3-B-2020-95 It examines, as a result meets regulation.
The study on the stability of test example two, yishenbugu liquid of the present invention
The yishenbugu liquid of Examples 1 to 3, comparative example 1~5 is stored 24 months under natural conditions, respectively at 3 The moon, 6 months, 12 months and 24 months measure pH value, check clarity, as a result see the table below 2.
The stability test result of 2 each yishenbugu liquid of table
From upper table 2：
(1) the yishenbugu liquid according to made from pharmacopeia technique of comparative example 1 checks there is a small amount of precipitation when storing 3 months, and 1~3 yishenbugu liquid of the embodiment of the present invention still keeps clarifying after placing 2 years, has no that precipitation generates, and pH value is basically unchanged, Stability is high.Therefore the present invention has been well solved after traditional yishenbugu liquid is deposited long by the improvement of preparation process and precipitated The problem of.
(2) using chitosan as fining agent, obtained yishenbugu liquid checked in having to measure at 12 months to sink comparative example 2 It forms sediment, and pH value declines；Using gelatin as fining agent, yishenbugu liquid obtained was checked at 6 months to be had on a small quantity comparative example 3 Precipitation, while pH value increases；It prompts chitosan and gelatin bad to the clarifying effect of radix paeoniae alba extraction of the present invention, is not particularly suited for In the present invention.
(3) comparative example 4 and comparative example 5 are respectively increased and reduce the pH value of product, and as a result its yishenbugu liquid exists respectively Being checked at 12 months and 6 months has precipitation to generate, and pH value is substantially change；The optimum pH model of prompting yishenbugu liquid of the present invention It is 4.0~4.4 to enclose, optimal pH 4.2, and can all system stability be had an impact by improving or reduce pH value.
The above content is a further detailed description of the present invention in conjunction with specific preferred embodiments, it is impossible to assert The specific implementation of the present invention is confined to these explanations.For those of ordinary skill in the art to which the present invention belongs, exist Under the premise of not departing from present inventive concept, several simple deduction or replace can also be made, should all be considered as belonging to the present invention's Protection domain.

Claims (6)

1. a kind of preparation method of yishenbugu liquid, which is characterized in that the Chinese medicine preparation of the yishenbugu liquid includes following heavy Measure the medicinal material of part meter：45 parts of the rhizome of davallia, 126 parts of the fleece-flower root, 63 parts of Poria cocos, 63 parts of teasel root, 44 parts of Radix Paeoniae Alba, 63 parts of Radix Angelicae Sinensis, Radix Codonopsis 16 parts of 75 parts, 63 parts of prepared rehmannia root, 63 parts of sealwort, 63 parts of the fruit of Chinese wolfberry, 45 parts of native copper and dried orange peel；
The preparation method includes the following steps：
S1, the rhizome of davallia for taking formula ratio, the fleece-flower root, Poria cocos, teasel root, Radix Angelicae Sinensis, Radix Codonopsis, prepared rehmannia root, sealwort, the fruit of Chinese wolfberry, native copper And dried orange peel, the water of medicinal material 4~6 times of weight of total weight is added in, is decocted 2 hours, filtration, filter residue adds in medicinal material 2~3 times of weights of total weight The water of amount decocts 2 hours, and filtration, merging filtrate is concentrated into every 1ml solution and is equivalent to crude drug 1g, adds 2 times of volumes of ethyl alcohol, Stirring, stands 16h at 5~10 DEG C, filters, and recycles ethyl alcohol, obtains mixing herbal extract；
S2, the Radix Paeoniae Alba for taking formula ratio add in the water of Radix Paeoniae Alba 2~4 times of weight of weight, decoct 2 hours, and filtration, filter residue adds in Radix Paeoniae Alba The water of 2~3 times of weight of weight decocts 2 hours, and filtration, merging filtrate is concentrated into every 1ml solution and is equivalent to crude drug 1g, obtains Concentrate；
S3, concentrate is taken, after adding in 1 times of volume dilution of water, obtains dilution, add in egg white solution, stir evenly, be slowly heated to It boils and is kept for 3~5 minutes, let cool, filtered, adjust pH value to 3.5~3.8, stand, filtration obtains white paeony root extract；
S4, herbal extract and white paeony root extract mixing will be mixed, and will add in 167 parts of sucrose, 20 parts of white wine and preservative, stirring is molten Xie Hou adjusts pH value to 4.0~4.4, and filtration adds water constant volume, stir evenly, embedding, sterilizing to get.
2. the preparation method of yishenbugu liquid as described in claim 1, which is characterized in that the concentration of volume percent of the ethyl alcohol It is 90~95%.
3. the preparation method of yishenbugu liquid as described in claim 1, which is characterized in that the addition volume of the egg white solution is The 2~4% of dilution volume.
4. the preparation method of yishenbugu liquid as claimed in claim 2, which is characterized in that the preservative includes benzoic acid 2.5 0.5 part of part and ethyl hydroxy benzoate.
5. the preparation method of yishenbugu liquid as described in claim 1, which is characterized in that in the preparation method, use citron Acid-sodium citrate buffer adjusts pH value.
6. yishenbugu liquid made from the preparation method of yishenbugu liquid as described in Claims 1 to 5 is any.
CN201810220941.4A 2018-03-16 2018-03-16 A kind of yishenbugu liquid and preparation method thereof Pending CN108210727A (en)

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Publication number Priority date Publication date Assignee Title
CN1368064A (en) * 2001-02-05 2002-09-11 杨孟君 Nano medicine 'yishenbugu' for tonifying kidney and strengthening bone and its preparing process
CN105998671A (en) * 2016-08-08 2016-10-12 程迪迪 Traditional Chinese medicine composition for treating cough and preparation method thereof
CN206762437U (en) * 2017-06-05 2017-12-19 亳州职业技术学院 A kind of Chinese medicine production filter

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