CN108208355A - A kind of particle feed that can improve bovine IVF embryos survival rate and preparation method thereof - Google Patents

A kind of particle feed that can improve bovine IVF embryos survival rate and preparation method thereof Download PDF

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CN108208355A
CN108208355A CN201711439330.0A CN201711439330A CN108208355A CN 108208355 A CN108208355 A CN 108208355A CN 201711439330 A CN201711439330 A CN 201711439330A CN 108208355 A CN108208355 A CN 108208355A
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parts
powder
rutin
survival rate
particle feed
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朱站英
梅雪芹
于宁
余洲
王斌斌
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HUZHOU HAIHUANG BIOTECHNOLOGY Co Ltd
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HUZHOU HAIHUANG BIOTECHNOLOGY Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/26—Compounds containing phosphorus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The present invention relates to a kind of particle feed that can improve bovine IVF embryos survival rate, the particle feed is mixed by following raw material components：Super steam fish meal, import chicken meal, euphausia superba powder, yellow worm powder, squid viscera powder, Se-enriched yeast powder, fermented bean dregs, soy protein concentrate, high-strength flour, product containing organic selenium, soyabean lecithin oil, fish oil, calcium dihydrogen phosphate, choline, carnitine, multi-vitamins, composite mineral matter, composite bacillus, with Extraction of rutin object.The feed link of the present invention crosses the digestibility that high temperature granulating improves raw material, and raw material is easily digested and assimilated by tadpole；Fish oil, phosphatide that some powders can not add etc., which can be added, can make the substance of Growth of Tadpoles；After Ultramicro-powder, the fineness 90% of feed crosses 80 mesh, meets the needs of tadpole bore is to feed granules；The liver and intestinal health of tadpole can be significantly improved, survival rate is high；Tadpole incidence is low, and abnormal rate is low, while the survival rate after metamorphosis is high.

Description

A kind of particle feed that can improve bovine IVF embryos survival rate and preparation method thereof
Technical field
The present invention relates to aquatic product matched field of fodder, refer in particular to a kind of particle feed that can improve bovine IVF embryos survival rate And preparation method thereof.
Background technology
Bullfrog (Rana catesbeiana), Chordata, Amphibia, Anura, Ranidae, Rana originate in the U.S. East, nineteen fifty-nine introduce China inland and carry out cultivation popularization, and all parts of the country are produced, and focus primarily upon Hubei and Hunan Provinces, Fujian, river at present The ground such as west, Sichuan.Have many advantages, such as that growth is fast, delicious flavour, full of nutrition, protein content is high, be rare food.Its micromicro Process hides, it is dirty can pharmacy, frog oil can make senior lubricant.The life cycle of bullfrog includes fertilized eggs, tadpole, froglet and into frog rank Section, wherein for the growth cycle in tadpole stage about at 70 days or so, the height of tadpole survival rate is that Buillfrog Raising is more crucial One of link, the price height of annual tadpole is very big, and sometimes big one seedling of specification seedling is hard to find, causes tadpole survival rate height Factor it is very much, wherein the most key is the operational administrative of raiser and the selection to tadpole bait.
The open-mouthed bait provided mostly currently on the market is without the powdery compound feed of puffing curing, is to choose mostly Raw material is concentrated to be prepared after carrying out mixing and micro mist, the benefit of this powder is that production operation is simple, but to the control of raw material It is required that very stringent, if raw material is stale, the liquid manure of tadpole cultivating pool does not get up or overfertilization, under the food ration horse back of tadpole Drop, then will appear the phenomena of mortality of tadpole.The present invention improve it is a kind of can improve bovine IVF embryos survival rate particle feed be Ultramicro grinding again after well doneization processing is prepared.Effect of the one Jing Guo high temperature and pressure, the nuisance inside raw material It is killed matter part；Secondly improving the digestibility of raw material, raw material is easily digested and assimilated by tadpole；Thirdly it can add Fish oil, phosphatide that powder can not add etc. can make the substance of Growth of Tadpoles；After its four Ultramicro-powder, the fineness 90% of raw material crosses 80 Mesh meets the needs of tadpole bore is to feed granules.
Invention content
The technical problems to be solved by the invention are that provide a kind of stable quality, harmful cause of disease for bovine IVF embryos growth period micro- The few biological feedstuff of biology, it meets tadpole period nutritional need, ensures the speed of growth and survival rate of tadpole, while grain size is big It is small suitable, in microgranular, meet tadpole and ingest requirement.
To achieve these goals, the present invention uses following technical scheme：
A kind of particle feed that can improve bovine IVF embryos survival rate, the particle feed by weight, by following raw material components It is mixed：Super steam fish meal 15-30 parts, 3-8 parts of import chicken meal, 3-10 parts of euphausia superba powder, yellow 3-8 parts of worm powder, squid 2-5 parts of fish guts powder, 3-6 parts of Se-enriched yeast powder, 8-15 parts of fermented bean dregs, 8-15 parts of soy protein concentrate, high-strength flour 16-20 Part, 3-6 parts of product containing organic selenium, 2.0-6.0 parts of soyabean lecithin oil, 1.0-3.0 parts of fish oil, 1.0-1.5 parts of calcium dihydrogen phosphate, choline 0.1-0.3 parts, 1-3 parts of carnitine, 0.5-1.0 parts of multi-vitamins, 1.0-2.0 parts of composite mineral matter, composite bacillus 1.0- 3.0 parts and 1-3.0 parts of Extraction of rutin object.
In the technical program, the prominent attractant effect to tadpole of the present invention, wherein euphausia superba powder, squid viscera powder, Tadpole can be allowed to ingest as early as possible containing food calling ingredient in dusty yeast.
Amino acid in containing 18 in yellow worm powder, content of fatty acid is high, is mostly unrighted acid, simultaneously containing abundant Minerals and vitamins, the most key immunity function that tadpole can be effectively improved containing chitin and antibacterial peptide promote immune The proliferation of cell, and antibacterial peptide is small with molecular weight, non-immunogenicity, has a broad antifungal spectrum etc. is specific, can effectively kill in tadpole body Gram-positive bacteria and negative bacterium, parasite etc..
Soyabean lecithin oil and fish oil：Phosphatide is the important component of biomembrane, be animal brain, nerve fiber, marrow and Indispensable component part in internal organ, it is extremely important to the growth and development of young animal.Fish oil is rich in omega-fatty acid, is children The indispensable fat source of body can improve the speed of growth of the young.
Preferably, the extracting method of Extraction of rutin object is：
a：Drying, crushing after rutin blade harvest, respectively plus 10 times of water is measured, and 8 times of 80-90 DEG C of amount decoction extractions 2 times, 1 is small every time When, merge decoction liquor, filtering, filtrate is concentrated into relative density 1.5；
b：In the filtrate of 1/3 concentration plus the zeolite powder of 5 times of amounts, mixing, drying obtain rutin crude extract A；
c：After residual filtrate is concentrated into relative density 1.8,0.3% chitosan flocks is added to refine, filter out supernatant, add in filtrate body The microbial fermentation solution of 3 times of product seals anaerobic fermentation 5-9d in plastic barrel；The obtained concentrated drying of zymotic fluid, obtains rutin Crude extract B；The microbial fermentation solution is pressed by saccharomycete, lactic acid bacteria, bacillus subtilis, Pasteur's acetobacter and photosynthetic bacteria Mixed fermentation is inoculated with according to 5: 1: 3: 6: 4 ratio, is made through 60-84h aerobic fermentations；
d：Rutin crude extract A with rutin crude extract B is merged, adds in containing for 10 times of rutin crude extract A and rutin crude extract B mass It in the ozone water solution for having mass fraction 0.03%, is concentrated and dried after sonic oscillation 30min, obtains Extraction of rutin object.
Preferably, every kilogram of vitamin premix contains：VA5-20mg, VD5-15mg, VE200-500mg, VC 800- 1500mg, VK15-25mg, VB130-50mg, VB2100-200mg, VB630-100mg, VB122-6mg, calcium pantothenate 100- 200mg, folic acid 5-15mg, niacinamide 20-80mg, biotin 10-20mg, inositol 200-500mg.
Preferably, every kilogram of mineral substance premix contains：Copper 1.89-4.53mg, iron 40.28-120.85mg, zinc 20.19- 80.75mg, manganese 3.64-29.14mg, cobalt 45.44-227.20mg, selenium 2.28-13.70mg, calcium 10.26-35.91mg, magnesium 103.85-311.54mg with iodine 32.75-114.01mg.
A kind of preparation method of particle feed that can improve bovine IVF embryos survival rate, the preparation method include following step Suddenly：
1) dispensing is with mixing：According to the basic composition and weight for being formulated big raw material, by super steam fish meal, import chicken meal, south Pole krill meal, yellow worm powder, squid viscera powder, Se-enriched yeast powder, fermented bean dregs, soy protein concentrate, high-strength flour, product containing organic selenium, Primary batching system and mixing are carried out, and adds in composite mineral matter, choline, calcium dihydrogen phosphate；
2) crush and add grease：Mixed material is subjected to coarse crushing, the second wheat-middlings are then carried out using micronizer It is broken；Enter secondary mix stages simultaneously, soyabean lecithin oil and fish oil are added in secondary mixing, and add in multi-vitamins, meat Alkali, composite bacillus and Extraction of rutin object；
3) it modulates and pelletizes：It is modulated by modulator, pelletizes into bulking machine；
4) it dries and cools down：Enter slat dryer by scraper plate to be dried, cooled down into cooler；
5) it crushes and packs, crushed with Ultramicro-powder, packaging and warehousing of weighing.
The beneficial effects of the invention are as follows：By the effect of high temperature and pressure, the harmful substance part inside raw material is killed；It is former Expect that attractant is strong, the digestibility of raw material is improved by high temperature granulating, raw material is easily digested and assimilated by tadpole；One can be added Fish oil, phosphatide that a little powders can not add etc. can make the substance of Growth of Tadpoles；After Ultramicro-powder, the fineness 90% of feed crosses 80 Mesh meets the needs of tadpole bore is to feed granules；The liver and intestinal health of tadpole can be significantly improved, survival rate is high；Tadpole Incidence is low, and abnormal rate is low, while the survival rate after metamorphosis is high；It is stronger than powder water stability, facilitate raiser to water The management of matter.
Specific embodiment
Below by specific embodiment, technical scheme of the present invention is described in further detail.
In the present invention, if not refering in particular to, used raw material and equipment etc. are commercially available or commonly used in the art. Method in following embodiments is the conventional method of this field unless otherwise instructed.
Embodiment 1：
A kind of particle feed that can improve bovine IVF embryos survival rate, the particle feed by weight, by following raw material components It is mixed：Super 15 parts of steam fish meal, 3 parts of import chicken meal, 3 parts of euphausia superba powder, yellow 3 parts of worm powder, squid viscera powder 2 Part, 3 parts of Se-enriched yeast powder, 8 parts of fermented bean dregs, 8 parts of soy protein concentrate, 16 parts of high-strength flour, 3 parts of product containing organic selenium, soybean lecithin 2.0 parts of oil, 1.0 parts of fish oil, 1.0 parts of calcium dihydrogen phosphate, 0.1 part of choline, 1 part of carnitine, 0.5 part of multi-vitamins, complex mineral 1 part of 1.0 parts of matter, 1.0 parts of composite bacillus and Extraction of rutin object.
The extracting method of Extraction of rutin object is：
a：Drying, crushing after rutin blade harvest, respectively plus 10 times of water is measured, and 8 times of 80-90 DEG C of amount decoction extractions 2 times, 1 is small every time When, merge decoction liquor, filtering, filtrate is concentrated into relative density 1.5；
b：In the filtrate of 1/3 concentration plus the zeolite powder of 5 times of amounts, mixing, drying obtain rutin crude extract A；
c：After residual filtrate is concentrated into relative density 1.8,0.3% chitosan flocks is added to refine, filter out supernatant, add in filtrate body The microbial fermentation solution of 3 times of product seals anaerobic fermentation 5-9d in plastic barrel；The obtained concentrated drying of zymotic fluid, obtains rutin Crude extract B；The microbial fermentation solution is pressed by saccharomycete, lactic acid bacteria, bacillus subtilis, Pasteur's acetobacter and photosynthetic bacteria Mixed fermentation is inoculated with according to 5: 1: 3: 6: 4 ratio, is made through 60-84h aerobic fermentations；
d：Rutin crude extract A with rutin crude extract B is merged, adds in containing for 10 times of rutin crude extract A and rutin crude extract B mass It in the ozone water solution for having mass fraction 0.03%, is concentrated and dried after sonic oscillation 30min, obtains Extraction of rutin object.
Every kilogram of vitamin premix contains：VA5-20mg, VD5-15mg, VE200-500mg, VC800-1500mg, VK 15-25mg, VB130-50mg, VB2100-200mg, VB630-100mg, VB122-6mg, calcium pantothenate 100-200mg, folic acid 5- 15mg, niacinamide 20-80mg, biotin 10-20mg, inositol 200-500mg.
Every kilogram of mineral substance premix contains：Copper 1.89-4.53mg, iron 40.28-120.85mg, zinc 20.19-80.75mg, Manganese 3.64-29.14mg, cobalt 45.44-227.20mg, selenium 2.28-13.70mg, calcium 10.26-35.91mg, magnesium 103.85- 311.54mg with iodine 32.75-114.01mg.
A kind of preparation method of particle feed that can improve bovine IVF embryos survival rate, the preparation method include following step Suddenly：
1) dispensing is with mixing：According to the basic composition and weight for being formulated big raw material, by super steam fish meal, import chicken meal, south Pole krill meal, yellow worm powder, squid viscera powder, Se-enriched yeast powder, fermented bean dregs, soy protein concentrate, high-strength flour, product containing organic selenium, Primary batching system and mixing are carried out, and adds in composite mineral matter, choline, calcium dihydrogen phosphate；
2) crush and add grease：Mixed material is subjected to coarse crushing, the second wheat-middlings are then carried out using micronizer It is broken；Enter secondary mix stages simultaneously, soyabean lecithin oil and fish oil are added in secondary mixing, and add in multi-vitamins, meat Alkali, composite bacillus and Extraction of rutin object；
3) it modulates and pelletizes：It is modulated by modulator, pelletizes into bulking machine；The masterplate grain size used is 1.0mm； It is 28% that base-material is modulated into moisture content in quality-adjusting device；Vapor tension control in the quality-adjusting device is 0.5MPa, and refining temperature is 90 DEG C, conditioning period 300s, at 95 DEG C, the speed of mainshaft control of bulking machine is 700r/min for the swelling temperature control of bulking machine, The bulking machine curing time is 95s；
4) it dries and cools down：Enter slat dryer by scraper plate to be dried, cooled down into cooler；
5) it crushes and packs, crushed with Ultramicro-powder to fineness 90% and cross 80 mesh, packaging and warehousing of weighing.
Embodiment 2
A kind of particle feed that can improve bovine IVF embryos survival rate, the particle feed by weight, by following raw material components It is mixed：Super 22 parts of steam fish meal, 5 parts of import chicken meal, 7 parts of euphausia superba powder, yellow 6 parts of worm powder, squid viscera powder 3 Part, 4 parts of Se-enriched yeast powder, 12 parts of fermented bean dregs, 13 parts of soy protein concentrate, high-strength flour 17,4 parts of product containing organic selenium, soybean lecithin 5 parts of oil, 2 parts of fish oil, 1.2 parts of calcium dihydrogen phosphate, 0.2 part of choline, 2 parts of carnitine, 0.6 part of multi-vitamins, composite mineral matter 1.7 Part, 2 parts of 1.8 parts of composite bacillus and Extraction of rutin object.
The extracting method of Extraction of rutin object is：
a：Drying, crushing after rutin blade harvest, respectively plus 10 times of water is measured, and 8 times of 80-90 DEG C of amount decoction extractions 2 times, 1 is small every time When, merge decoction liquor, filtering, filtrate is concentrated into relative density 1.5；
b：In the filtrate of 1/3 concentration plus the zeolite powder of 5 times of amounts, mixing, drying obtain rutin crude extract A；
c：After residual filtrate is concentrated into relative density 1.8,0.3% chitosan flocks is added to refine, filter out supernatant, add in filtrate body The microbial fermentation solution of 3 times of product seals anaerobic fermentation 5-9d in plastic barrel；The obtained concentrated drying of zymotic fluid, obtains rutin Crude extract B；The microbial fermentation solution is pressed by saccharomycete, lactic acid bacteria, bacillus subtilis, Pasteur's acetobacter and photosynthetic bacteria Mixed fermentation is inoculated with according to 5: 1: 3: 6: 4 ratio, is made through 60-84h aerobic fermentations；
d：Rutin crude extract A with rutin crude extract B is merged, adds in containing for 10 times of rutin crude extract A and rutin crude extract B mass It in the ozone water solution for having mass fraction 0.03%, is concentrated and dried after sonic oscillation 30min, obtains Extraction of rutin object.
Every kilogram of vitamin premix contains：VA5-20mg, VD5-15mg, VE200-500mg, Vc800-1500mg, VK 15-25mg, VB130-50mg, VB2100-200mg, VB630-100mg, VB122-6mg, calcium pantothenate 100-200mg, folic acid 5- 15mg, niacinamide 20-80mg, biotin 10-20mg, inositol 200-500mg.
Every kilogram of mineral substance premix contains：Copper 1.89-4.53mg, iron 40.28-120.85mg, zinc 20.19-80.75mg, Manganese 3.64-29.14mg, cobalt 45.44-227.20mg, selenium 2.28-13.70mg, calcium 10.26-35.91mg, magnesium 103.85- 311.54mg with iodine 32.75-114.01mg.
A kind of preparation method of particle feed that can improve bovine IVF embryos survival rate, the preparation method include following step Suddenly：
1) dispensing is with mixing：According to the basic composition and weight for being formulated big raw material, by super steam fish meal, import chicken meal, south Pole krill meal, yellow worm powder, squid viscera powder, Se-enriched yeast powder, fermented bean dregs, soy protein concentrate, high-strength flour, product containing organic selenium, Primary batching system and mixing are carried out, and adds in composite mineral matter, choline, calcium dihydrogen phosphate；
2) crush and add grease：Mixed material is subjected to coarse crushing, the second wheat-middlings are then carried out using micronizer It is broken；Enter secondary mix stages simultaneously, soyabean lecithin oil and fish oil are added in secondary mixing, and add in multi-vitamins, meat Alkali, composite bacillus and Extraction of rutin object；
3) it modulates and pelletizes：It is modulated by modulator, pelletizes into bulking machine；The masterplate grain size used is 1.0mm； It is 28% that base-material is modulated into moisture content in quality-adjusting device；Vapor tension control in the quality-adjusting device is 0.5MPa, and refining temperature is 90 DEG C, conditioning period 300s, at 95 DEG C, the speed of mainshaft control of bulking machine is 700r/min for the swelling temperature control of bulking machine, The bulking machine curing time is 95s；
4) it dries and cools down：Enter slat dryer by scraper plate to be dried, cooled down into cooler；
5) it crushes and packs, crushed with Ultramicro-powder to fineness 90% and cross 80 mesh, packaging and warehousing of weighing.
Embodiment 3
A kind of particle feed that can improve bovine IVF embryos survival rate, the particle feed by weight, by following raw material components It is mixed：Super 30 parts of steam fish meal, 8 parts of import chicken meal, 10 parts of euphausia superba powder, yellow 8 parts of worm powder, squid viscera powder 5 Part, 6 parts of Se-enriched yeast powder, 15 parts of fermented bean dregs, 15 parts of soy protein concentrate, 20 parts of high-strength flour, 6 parts of product containing organic selenium, soybean phosphorus 6.0 parts of fatty oil, 3.0 parts of fish oil, 1.5 parts of calcium dihydrogen phosphate, 0.3 part of choline, 3 parts of carnitine, 1.0 parts of multi-vitamins, grandidierite 3.0 parts of 2.0 parts of substance, 3.0 parts of composite bacillus and Extraction of rutin object.
The extracting method of Extraction of rutin object is：
a：Drying, crushing after rutin blade harvest, respectively plus 10 times of water is measured, and 8 times of 80-90 DEG C of amount decoction extractions 2 times, 1 is small every time When, merge decoction liquor, filtering, filtrate is concentrated into relative density 1.5；
b：In the filtrate of 1/3 concentration plus the zeolite powder of 5 times of amounts, mixing, drying obtain rutin crude extract A；
c：After residual filtrate is concentrated into relative density 1.8,0.3% chitosan flocks is added to refine, filter out supernatant, add in filtrate body The microbial fermentation solution of 3 times of product seals anaerobic fermentation 5-9d in plastic barrel；The obtained concentrated drying of zymotic fluid, obtains rutin Crude extract B；The microbial fermentation solution is pressed by saccharomycete, lactic acid bacteria, bacillus subtilis, Pasteur's acetobacter and photosynthetic bacteria Mixed fermentation is inoculated with according to 5: 1: 3: 6: 4 ratio, is made through 60-84h aerobic fermentations；
d：Rutin crude extract A with rutin crude extract B is merged, adds in containing for 10 times of rutin crude extract A and rutin crude extract B mass It in the ozone water solution for having mass fraction 0.03%, is concentrated and dried after sonic oscillation 30min, obtains Extraction of rutin object.
Every kilogram of vitamin premix contains：VA5-20mg, VD5-15mg, VE200-500mg, VC800-1500mg, VK 15-25mg, VB130-50mg, VB2100-200mg, VB630-100mg, VB122-6mg, calcium pantothenate 100-200mg, folic acid 5- 15mg, niacinamide 20-80mg, biotin 10-20mg, inositol 200-500mg.
Every kilogram of mineral substance premix contains：Copper 1.89-4.53mg, iron 40.28-120.85mg, zinc 20.19-80.75mg, Manganese 3.64-29.14mg, cobalt 45.44-227.20mg, selenium 2.28-13.70mg, calcium 10.26-35.91mg, magnesium 103.85- 311.54mg with iodine 32.75-114.01mg.
A kind of preparation method of particle feed that can improve bovine IVF embryos survival rate, the preparation method include following step Suddenly：
1) dispensing is with mixing：According to the basic composition and weight for being formulated big raw material, by super steam fish meal, import chicken meal, south Pole krill meal, yellow worm powder, squid viscera powder, Se-enriched yeast powder, fermented bean dregs, soy protein concentrate, high-strength flour, product containing organic selenium, Primary batching system and mixing are carried out, and adds in composite mineral matter, choline, calcium dihydrogen phosphate；
2) crush and add grease：Mixed material is subjected to coarse crushing, the second wheat-middlings are then carried out using micronizer It is broken；Enter secondary mix stages simultaneously, soyabean lecithin oil and fish oil are added in secondary mixing, and add in multi-vitamins, meat Alkali, composite bacillus and Extraction of rutin object；
3) it modulates and pelletizes：It is modulated by modulator, pelletizes into bulking machine；The masterplate grain size used is 1.0mm； It is 28% that base-material is modulated into moisture content in quality-adjusting device；Vapor tension control in the quality-adjusting device is 0.5MPa, and refining temperature is 90 DEG C, conditioning period 300s, at 95 DEG C, the speed of mainshaft control of bulking machine is 700r/min for the swelling temperature control of bulking machine, The bulking machine curing time is 95s；
4) it dries and cools down：Enter slat dryer by scraper plate to be dried, cooled down into cooler；
5) it crushes and packs, crushed with Ultramicro-powder to fineness 90% and cross 80 mesh, packaging and warehousing of weighing.
The bovine IVF embryos feed provided in 1-3 of the embodiment of the present invention by the way that example is cultivated to prove can meet Growth of Tadpoles and send out The nutritional need educated, comparative example use commercially available mixed feed；The metamorphosis that the particle feed of the present invention can improve tadpole survives Rate is improved up to more than 12%, while facilitates the management of tadpole period water quality, not perishable water.It the results are shown in Table 1.
Table 1, comparing result
Hatch survival rate/% Period of metamorphosis survival rate/%
Embodiment 1 95 60
Embodiment 2 96 61
Embodiment 3 97 73
Comparative example 1 85 42
Embodiment described above is a kind of preferable scheme of the present invention, not makees limit in any form to the present invention System also has other variants and remodeling under the premise of without departing from the technical solution recorded in claim.

Claims (5)

1. a kind of particle feed that can improve bovine IVF embryos survival rate, which is characterized in that the particle feed by weight, by Following raw material components are mixed：Super steam fish meal 15-30 parts, 3-8 parts of import chicken meal, 3-10 parts of euphausia superba powder, Huang 3-8 parts of worm powder, 2-5 parts of squid viscera powder, 3-6 parts of Se-enriched yeast powder, 8-15 parts of fermented bean dregs, 8-15 parts of soy protein concentrate, 16-20 parts of high-strength flour, 3-6 parts of product containing organic selenium, 2.0-6.0 parts of soyabean lecithin oil, 1.0-3.0 parts of fish oil, calcium dihydrogen phosphate 1.0- 1.5 parts, 0.1-0.3 parts of choline, 1-3 parts of carnitine, 0.5-1.0 parts of multi-vitamins, 1.0-2.0 parts of composite mineral matter, compound bud 1-3.0 parts of 1.0-3.0 parts of spore bacillus and Extraction of rutin object.
2. a kind of particle feed that can improve bovine IVF embryos survival rate according to claim 1, which is characterized in that rutin carries The extracting method for taking object is：
a：Drying, crushing after rutin blade harvest, respectively plus 10 times of water is measured, and 8 times of 80-90 DEG C of amount decoction extractions 2 times, 1 is small every time When, merge decoction liquor, filtering, filtrate is concentrated into relative density 1.5；
b：In the filtrate of 1/3 concentration plus the zeolite powder of 5 times of amounts, mixing, drying obtain rutin crude extract A；
c：After residual filtrate is concentrated into relative density 1.8,0.3% chitosan flocks is added to refine, filter out supernatant, add in filtrate body The microbial fermentation solution of 3 times of product seals anaerobic fermentation 5-9d in plastic barrel；The obtained concentrated drying of zymotic fluid, obtains rutin Crude extract B；The microbial fermentation solution is pressed by saccharomycete, lactic acid bacteria, bacillus subtilis, Pasteur's acetobacter and photosynthetic bacteria Mixed fermentation is inoculated with according to 5: 1: 3: 6: 4 ratio, is made through 60-84h aerobic fermentations；
d：Rutin crude extract A with rutin crude extract B is merged, adds in containing for 10 times of rutin crude extract A and rutin crude extract B mass It in the ozone water solution for having mass fraction 0.03%, is concentrated and dried after sonic oscillation 30min, obtains Extraction of rutin object.
3. a kind of particle feed that can improve bovine IVF embryos survival rate according to claim 1, which is characterized in that every kilogram Vitamin premix contains：VA5-20mg, VD5-15mg, VE200-500mg, VC800-1500mg, VK15-25mg, VB1 30-50mg, VB2100-200mg, VB630-100mg, VB122-6mg, calcium pantothenate 100-200mg, folic acid 5-15mg, niacinamide 20-80mg, biotin 10-20mg, inositol 200-500mg.
4. a kind of particle feed that can improve bovine IVF embryos survival rate according to claim 1, which is characterized in that every kilogram Mineral substance premix contains：Copper 1.89-4.53mg, iron 40.28-120.85mg, zinc 20.19-80.75mg, manganese 3.64-29.14mg, Cobalt 45.44-227.20mg, selenium 2.28-13.70mg, calcium 10.26-35.91mg, magnesium 103.85-311.54mg and iodine 32.75- 114.01mg。
5. a kind of preparation method of particle feed as described in claim 1 that can improve bovine IVF embryos survival rate, feature exist In the preparation method includes the following steps：
1) dispensing is with mixing：According to the basic composition and weight for being formulated big raw material, by super steam fish meal, import chicken meal, south Pole krill meal, yellow worm powder, squid viscera powder, Se-enriched yeast powder, fermented bean dregs, soy protein concentrate, high-strength flour, product containing organic selenium, Primary batching system and mixing are carried out, and adds in composite mineral matter, choline, calcium dihydrogen phosphate；
2) crush and add grease：Mixed material is subjected to coarse crushing, the second wheat-middlings are then carried out using micronizer It is broken；Enter secondary mix stages simultaneously, soyabean lecithin oil and fish oil are added in secondary mixing, and add in multi-vitamins, meat Alkali, composite bacillus and Extraction of rutin object；
3) it modulates and pelletizes：It is modulated by modulator, plasmid is carried out into bulking machine；
4) it dries and cools down：Enter slat dryer by scraper plate to be dried, cooled down into cooler；
5) it crushes and packs, crushed with Ultramicro-powder, packaging and warehousing of weighing.
CN201711439330.0A 2017-12-26 2017-12-26 A kind of particle feed that can improve bovine IVF embryos survival rate and preparation method thereof Pending CN108208355A (en)

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