CN108202081A - A kind of method for improveing photosynthetic microbial inoculum containment plant absorption organic poison cypermethrin - Google Patents

A kind of method for improveing photosynthetic microbial inoculum containment plant absorption organic poison cypermethrin Download PDF

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CN108202081A
CN108202081A CN201810180291.5A CN201810180291A CN108202081A CN 108202081 A CN108202081 A CN 108202081A CN 201810180291 A CN201810180291 A CN 201810180291A CN 108202081 A CN108202081 A CN 108202081A
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soil
plant
basin
microbial inoculum
organic
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王艺璇
赵远
张玉虎
肖娴
赵利华
符菁
张芸
张徐洁
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Changzhou University
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Changzhou University
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Soil Sciences (AREA)
  • Environmental & Geological Engineering (AREA)
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Abstract

The invention discloses one kind using rice and Alfalfa as research object, study photosynthetic microbial inoculum and check rice and inhale the method that Alfalfa receives the actual effect of organic poison cypermethrin, belong to organic polluted soil recovery technique field.Its step is：Quantitative soil is subjected to organic matter contamination, it carries out applying fertilizer and the photosynthetic microbial inoculum of addition again, using rice cultivation and Alfalfa sample collection is carried out in the stipulated time, by measuring the ground of plant, sub-surface length, fresh weight, and in the pollutant accumulation amount and basin of whole strain, cauline leaf and root system soil organic pollution content, find a kind of restorative procedure for being more suitable for organic polluted soil.The present invention has the advantages that low cost, high stable, easy to operate, non-secondary pollution generate, while small to the destruction of soil texture, and certain science and practical basis are provided for soil remediation.

Description

A kind of method for improveing photosynthetic microbial inoculum containment plant absorption organic poison cypermethrin
Technical field
The present invention relates to a kind of methods for improveing photosynthetic microbial inoculum containment plant absorption organic poison cypermethrin, are organic dirts Contaminate technical field of soil remediation.
Background technology
With China's expanding economy, the organic chemicals being discharged into natural environment are more and more, wherein most into Entered soil environment, soil ecosystem into the maximum receptor of organic pollution or even some organic pollutions have toxicity, Bioconcentration can persistently exist in soil environment, increasing to the harm of ecological environment and human health.Have in soil Machine pollutant mainly includes volatile organic contaminant and half volatile organic contaminant.China's soil organic pollutant it is main Type includes：Petroleum hydrocarbon class pollutant, halogenated hydrocarbon pollutant, pesticide pollutant, polycyclic aromatic hydrocarbon, Polychlorinated biphenyls, bioxin, The organic pollutions such as phthalic acid ester.Soil organic pollutant can directly be taken in by human body, in some instances it may even be possible to accumulate in vivo, shadow Human body biochemistry and physiological reaction are rung, so as to influence metabolism, development and reproductive function, it is also possible to influence the intellectual development water of people It is flat, destroy nervous system and internal system.Soil organic pollutant is to the metabolism of soil animal, hereditary capacity and to planting The influence of the growth and development of object destroys ecological environment, so as to indirect hazard human health.Organic pollution of soil is dirty with air The environmental problems such as dye, water pollution are closely related.Soil organic pollutant easily enters air under the action of wind-force and waterpower In water body, lead to other secondary ecological problems such as atmosphere pollution, water pollution and ecosystem degradation.
The invention discloses one kind using rice and Alfalfa as research object, to study photosynthetic microbial inoculum and check rice and suction The method that Alfalfa receives the actual effect of organic poison cypermethrin, belongs to organic polluted soil recovery technique field.Its step For：Quantitative soil is subjected to organic matter contamination, then carries out applying fertilizer and the photosynthetic microbial inoculum of addition, utilizes rice cultivation and lucerne Mu grass carries out sample collection in the stipulated time, by the ground, sub-surface length, fresh weight and whole strain, cauline leaf and the root that measure plant The organic pollution content of soil in the pollutant accumulation amount and basin of system finds a kind of reparation side for being more suitable for organic polluted soil Method.The present invention has the advantages that low cost, high stable, easy to operate, non-secondary pollution generate, while to the destruction of soil texture It is small, provide certain science and practical basis for soil remediation.
Invention content
The present invention studies photosynthetic microbial inoculum containment plant absorption organic poison chlorine for organic matter cypermethrin contaminated soil The method of the actual effect of Cyano chrysanthemate.
In order to solve the above-mentioned technical problem, the present invention adopts the following technical scheme that：
1. soil quantifies
2. organic matter is contaminated
3. soil application
4. the photosynthetic microbial inoculum of addition
5. planting
6. sample collection
7. testing index
It is of the invention compared with other methods, it is beneficial that：
The photosynthetic bacteria of use is distributed widely in the soil of nature, paddy field, marsh, lake, Jiang Hai etc., and it is wide to obtain source General, the light and heat that it is received using soil is the main power of rich soil and promotion animal and plant growth, photosynthetic bacteria is always as the energy In terms of for production and living and plant growth, but someone does not study yet in terms of plant-microorganism remediation heavy metal, therefore has There is novelty.And rice is the major economic crops of China and people's essential food daily, research photosynthetic bacteria containment is planted Object absorbs organic pollution, production to rice and edible has huge practical significance.The present invention utilizes low cost, Gao Wen The characteristics of fixed, easy to operate, non-secondary pollution generates and is small to the destruction of soil texture, provides certain for soil remediation It while science and practical basis remediating heavy metal soil, and can guarantee the fertile Chengdu of soil, ecological environment played more Positive effect.
Specific embodiment
A kind of method for improveing photosynthetic microbial inoculum containment plant absorption organic poison cypermethrin, step include：
(1) soil is quantitative：The soil of acquisition is crossed into 20 mesh sieve, its moisture content is surveyed and fills basin；Big basin is 2.5kg soil (with dry Soil meter) rice cultivation；Small basin is native (in terms of dry ground) Planting Medicago sativa grass of 1kg.
(2) organic matter is contaminated：Tri- setting 0,0.3,0.5mg/kg gradients, weigh appropriate cypermethrin and are dissolved in 1L acetone In, 74.2ml is added after being completely dissolved per basin.
(3) soil application：
With pure (NH2) 2CO is analyzed, KH2PO4, K2SO4 apply base fertilizer N：0.15g/kg soil, P2O5：0.05g/kg soil, K2O：0.15g/kg soil.
(4) photosynthetic microbial inoculum addition：
Three setting 0,30,40kg/ mus gradients.Note：Bacterium solution is added in plantation.
(5) planting：
Rice is sowed seeds 15 per basin, Alfalfa is sowed seeds 80 per basin.
(6) sample collection：
(root needs to wash after first impregnating 15min with 20mmolL-1EDTA solution for the overground part of harvest plant and root respectively Only), clean plant is put into thermostatic drying chamber after 105 DEG C of water-removings 30min, 70 DEG C of dry 48h and preserved, and with measuring plant Top dry weight.While harvesting plant, pedotheque is acquired respectively, will be preserved after pedotheque natural air drying.
Sampling time first time：After plant growth 2 months
Second of sampling time：After plant growth 4 months
(7) testing index：
Sampling every time, often processing take ground, sub-surface length, fresh weight and whole strain, cauline leaf and the root system of 5 plants of measure plant Pollutant accumulation amount；Soil in the processing basin is taken, measures its pollutant load.
This research is based on the basis of the research of domestic and international correlation technique, using rice and Alfalfa as research object, is studied Photosynthetic microbial inoculum checks rice and inhales the method that Alfalfa receives the actual effect of organic poison cypermethrin, and quantitative soil is carried out Organic matter is contaminated, then carries out applying fertilizer and the photosynthetic microbial inoculum of addition, is carried out using rice cultivation and Alfalfa in the stipulated time Sample collection, by measure the ground of plant, sub-surface length, fresh weight and whole strain, cauline leaf and root system pollutant accumulation amount and The organic pollution content of soil in basin finds a kind of restorative procedure for being more suitable for organic polluted soil.
Embodiment 1：
A kind of method for improveing photosynthetic microbial inoculum containment plant absorption organic poison cypermethrin, step include：
(1) soil is quantitative：The soil of acquisition is crossed into 20 mesh sieve, its moisture content is surveyed and fills basin；Big basin is 2.5kg soil (with dry Soil meter) rice cultivation；Small basin is native (in terms of dry ground) Planting Medicago sativa grass of 1kg.
(2) organic matter is contaminated：Tri- setting 0,0.3,0.5mg/kg gradients, weigh appropriate cypermethrin and are dissolved in 1L acetone In, 74.2ml is added after being completely dissolved per basin.
(3) soil application：
With the pure (NH of analysis2)2CO, KH2PO4, K2SO4Apply base fertilizer N：0.3g/kg soil, P2O5：0.1g/kg soil, K2O： 0.3g/kg soil.
(4) photosynthetic microbial inoculum addition：
Three setting 0,30,60kg/ mus gradients.Note：Bacterium solution is added in plantation.
(5) planting：
Rice is sowed seeds 15 per basin, Alfalfa is sowed seeds 80 per basin.
(6) sample collection：
(root needs to wash after first impregnating 15min with 20mmolL-1EDTA solution for the overground part of harvest plant and root respectively Only), clean plant is put into thermostatic drying chamber after 105 DEG C of water-removings 30min, 70 DEG C of dry 48h and preserved, and with measuring plant Top dry weight.While harvesting plant, pedotheque is acquired respectively, will be preserved after pedotheque natural air drying.
Sampling time first time：After plant growth 2 months
Second of sampling time：After plant growth 4 months
(7) testing index：
Sampling every time, often processing take ground, sub-surface length, fresh weight and whole strain, cauline leaf and the root system of 5 plants of measure plant Pollutant accumulation amount；Soil in the processing basin is taken, measures its pollutant load.
Embodiment 2：
A kind of method for improveing photosynthetic microbial inoculum containment plant absorption organic poison cypermethrin, step include：
(1) soil is quantitative：The soil of acquisition is crossed into 20 mesh sieve, its moisture content is surveyed and fills basin；Big basin is 2.5kg soil (with dry Soil meter) rice cultivation；Small basin is native (in terms of dry ground) Planting Medicago sativa grass of 1kg.
(2) organic matter is contaminated：Tri- setting 0,0.3,0.5mg/kg gradients, weigh appropriate cypermethrin and are dissolved in 1L acetone In, 74.2ml is added after being completely dissolved per basin.
(3) soil application：
With the pure (NH of analysis2)2CO, KH2PO4, K2SO4Apply base fertilizer N：0.075g/kg soil, P2O5：0.025g/kg soil, K2O： 0.075g/kg soil.
(4) photosynthetic microbial inoculum addition：
Three setting 0,20,40kg/ mus gradients.Note：Bacterium solution is added in plantation.
(5) planting：
Rice is sowed seeds 15 per basin, Alfalfa is sowed seeds 80 per basin.
(6) sample collection：
(root needs to wash after first impregnating 15min with 20mmolL-1EDTA solution for the overground part of harvest plant and root respectively Only), clean plant is put into thermostatic drying chamber after 105 DEG C of water-removings 30min, 70 DEG C of dry 48h and preserved, and with measuring plant Top dry weight.While harvesting plant, pedotheque is acquired respectively, will be preserved after pedotheque natural air drying.
Sampling time first time：After plant growth 2 months
Second of sampling time：After plant growth 4 months
(7) testing index：
Sampling every time, often processing take ground, sub-surface length, fresh weight and whole strain, cauline leaf and the root system of 5 plants of measure plant Pollutant accumulation amount；Soil in the processing basin is taken, measures its pollutant load.
Embodiment 3：
A kind of method for improveing photosynthetic microbial inoculum containment plant absorption organic poison cypermethrin, step include：
(1) soil is quantitative：The soil of acquisition is crossed into 20 mesh sieve, its moisture content is surveyed and fills basin；Big basin is 2.5kg soil (with dry Soil meter) rice cultivation；Small basin is native (in terms of dry ground) Planting Medicago sativa grass of 1kg.
(2) organic matter is contaminated：Tri- setting 0,0.3,0.5mg/kg gradients, weigh appropriate cypermethrin and are dissolved in 1L acetone In, 74.2ml is added after being completely dissolved per basin.
(3) soil application：
With the pure (NH of analysis2)2CO, KH2PO4, K2SO4Apply base fertilizer N：0.45g/kg soil, P2O5：0.15g/kg soil, K2O： 0.45g/kg soil.
(4) photosynthetic microbial inoculum addition：
Three setting 0,40,80kg/ mus gradients.Note：Bacterium solution is added in plantation.
(5) planting：
Rice is sowed seeds 15 per basin, Alfalfa is sowed seeds 80 per basin.
(6) sample collection：
(root needs to wash after first impregnating 15min with 20mmolL-1EDTA solution for the overground part of harvest plant and root respectively Only), clean plant is put into thermostatic drying chamber after 105 DEG C of water-removings 30min, 70 DEG C of dry 48h and preserved, and with measuring plant Top dry weight.While harvesting plant, pedotheque is acquired respectively, will be preserved after pedotheque natural air drying.
Sampling time first time：After plant growth 2 months
Second of sampling time：After plant growth 4 months
(7) testing index：
Sampling every time, often processing take ground, sub-surface length, fresh weight and whole strain, cauline leaf and the root system of 5 plants of measure plant Pollutant accumulation amount；Soil in the processing basin is taken, measures its pollutant load.
Embodiment described above is only that the preferred embodiment of the present invention is described, not to the model of the present invention It encloses and is defined, under the premise of the design spirit for not departing from the present invention, the technical staff of this field ordinary skill is to the present invention The various modifications made of technical solution and improvement, should all fall into the protection domain that claims of the present invention determines.

Claims (3)

  1. A kind of 1. method for improveing photosynthetic microbial inoculum containment plant absorption organic poison cypermethrin, it is characterised in that have following real Apply step：
    (1) soil is quantitative：The soil of acquisition is crossed into 20 mesh sieve, its moisture content is surveyed and fills basin；Big basin is native (in terms of dry ground) for 2.5kg Rice cultivation；Small basin is native (in terms of dry ground) Planting Medicago sativa grass of 1kg.
    (2) organic matter is contaminated：Tri- setting 0,0.3,0.5mg/kg gradients, weigh appropriate cypermethrin and are dissolved in 1L acetone, complete 74.2ml is added after fully dissolved per basin.
    (3) soil application：
    With the pure (NH of analysis2)2CO, KH2PO4, K2SO4Apply base fertilizer N：0.15g/kg soil, P2O5：0.05g/kg soil, K2O：0.15g/ Kg soil.
    (4) photosynthetic microbial inoculum addition：
    Three setting 0,30,40kg/ mus gradients.Note：Bacterium solution is added in plantation.
    (5) planting：
    Rice is sowed seeds 15 per basin, Alfalfa is sowed seeds 80 per basin.
    (6) sample collection：
    The overground part of harvest plant and root (being cleaned after first need to impregnating 15min with 20mmolL-1EDTA solution root) respectively, Clean plant is put into thermostatic drying chamber after 105 DEG C of water-removings 30min, 70 DEG C of dry 48h and is preserved, and measure plant above ground portion Dry weight.While harvesting plant, pedotheque is acquired respectively, will be preserved after pedotheque natural air drying.
    Sampling time first time：After plant growth 2 months
    Second of sampling time：After plant growth 4 months
    (7) testing index：
    Sampling every time, often processing take the dirt of 5 plants of grounds for measuring plant, sub-surface length, fresh weight and whole strain, cauline leaf and root system Contaminate object accumulation；Soil in the processing basin is taken, measures its pollutant load.
  2. 2. a kind of method for improveing photosynthetic microbial inoculum containment plant absorption organic poison cypermethrin according to claim 1, It is characterized in that：The photosynthetic bacteria of use is distributed widely in the soil of nature, paddy field, marsh, lake, Jiang Hai etc., obtains and Source is extensive, it using soil receive light and heat as the energy, be rich soil and promote animal and plant growth main power, photosynthetic bacteria Always for production and living and plant growth aspect, but someone does not study yet in terms of plant-microorganism remediation heavy metal, Therefore with novelty.
  3. 3. a kind of method for improveing photosynthetic microbial inoculum containment plant absorption organic poison cypermethrin according to claim 1, It is characterized in that：Rice is the major economic crops of China and people's essential food daily, and research photosynthetic bacteria containment is planted Object absorbs organic pollution, production to rice and edible has huge practical significance.The present invention utilizes low cost, Gao Wen The characteristics of fixed, easy to operate, non-secondary pollution generates and is small to the destruction of soil texture, provides certain for soil remediation It while science and practical basis remediating heavy metal soil, and can guarantee the fertile Chengdu of soil, ecological environment played more Positive effect.
CN201810180291.5A 2018-03-05 2018-03-05 A kind of method for improveing photosynthetic microbial inoculum containment plant absorption organic poison cypermethrin Pending CN108202081A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103833476A (en) * 2014-03-21 2014-06-04 润禾泰华生物科技（北京）有限公司 Special liquid fertilizer for increasing yield of strawberry, killing insects and degrading pesticide residues and preparation method thereof
CN104692969A (en) * 2015-02-14 2015-06-10 姜华 Biological humic acid liquid fertilizer and preparation method thereof
CN103861869B (en) * 2014-04-05 2015-08-19 南开大学 A kind of restorative procedure of cypermethrin contaminated soil
CN107244999A (en) * 2017-05-24 2017-10-13 攀枝花市绿态美生物科技有限公司 It is a kind of to remove microbial bacterial agent of removing heavy metals and its preparation method and application
CN105903148B (en) * 2016-05-24 2019-02-19 四川大学 Can efficient degradation cypermethrin and 3- phenoxy benzoic acid wettable bacteria powder and preparation method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103833476A (en) * 2014-03-21 2014-06-04 润禾泰华生物科技（北京）有限公司 Special liquid fertilizer for increasing yield of strawberry, killing insects and degrading pesticide residues and preparation method thereof
CN103861869B (en) * 2014-04-05 2015-08-19 南开大学 A kind of restorative procedure of cypermethrin contaminated soil
CN104692969A (en) * 2015-02-14 2015-06-10 姜华 Biological humic acid liquid fertilizer and preparation method thereof
CN105903148B (en) * 2016-05-24 2019-02-19 四川大学 Can efficient degradation cypermethrin and 3- phenoxy benzoic acid wettable bacteria powder and preparation method
CN107244999A (en) * 2017-05-24 2017-10-13 攀枝花市绿态美生物科技有限公司 It is a kind of to remove microbial bacterial agent of removing heavy metals and its preparation method and application

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张杏丽等: "镉-八氯代二苯并呋喃复合污染土壤中紫茉莉对镉的修复能力", <<环境科学>> *

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Application publication date: 20180626