CN108196082A - A kind of high speed platelet function assay instrument and method - Google Patents
A kind of high speed platelet function assay instrument and method Download PDFInfo
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- CN108196082A CN108196082A CN201810280462.1A CN201810280462A CN108196082A CN 108196082 A CN108196082 A CN 108196082A CN 201810280462 A CN201810280462 A CN 201810280462A CN 108196082 A CN108196082 A CN 108196082A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00594—Quality control, including calibration or testing of components of the analyser
- G01N35/00613—Quality control
- G01N35/00623—Quality control of instruments
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00594—Quality control, including calibration or testing of components of the analyser
- G01N35/00693—Calibration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
Abstract
The present invention provides a kind of high speed platelet function assay instrument and method, instrument includes Special sample Transmission system, more than one independent detection unit and control unit.The Special sample Transmission system passes through each detection unit for transmitting multiple sample connection cups simultaneously.The instrument is equipped with the detection unit of platelet counts and volume in more than one detection blood sample along Special sample Transmission system.Instrument continues mixing each sample also during sample transfer.After each detection unit of the instrument detects identical quality-control product, unification is carried out to each detection unit automatically and is automatically corrected, it is ensured that each detection unit testing result consistency of instrument.Instrument passes through to adding in before and after induced polymerization inhibitor and induced polymerization inhibitor being added to calculate platelet function in the blood sample with being not added with the platelet counts that induced polymerization inhibitor blood examination obtains.
Description
Technical field
The present invention relates to medical inspection field, particularly a kind of high speed platelet function assay instrument and method.
Background technology
Existing platelet function assay instrument has compared with previous other Platelet function Analyzers compared with much progress.But make
Also there are certain deficiencies in.Detection needs to expend the more time, and often a detection needs 10 minutes, and automate journey
Degree is not high, can not meet clinical position needs.
Invention content
Goal of the invention：The technical problems to be solved by the invention are in view of the deficiencies of the prior art, to provide a kind of high speed blood
Platelet function detecting instrument and method.
In order to solve the above technical problem, the present invention provides a kind of high speed platelet function assay instrument and method, the instrument
Device includes a Special sample Transmission system, more than one independent detection unit, control unit composition, the Special sample transmission
For system for transmitting multiple specimen cups simultaneously one by one by each detection unit, each detection unit passes through the detection in each specimen cup
Blood sample during unit in each hole of pipette samples cup, and the platelet counts in each blood sample, volume are detected respectively.Each detection unit obtains
The platelet counts added in for identical blood sample before and after induced polymerization inhibitor are obtained, pass through the comparison to adding in induced polymerization inhibitor Platelet quantity
Obtain the platelet function level value of the blood sample.
In the present invention, the detection unit of the instrument is there are one more than, and there are one agent dispensing units for tool.Selected by instrument
Specimen cup has multiple holes, and each each hole of specimen cup is mutual indepedent and is respectively previously charged into the identical blood sample of equivalent, wherein
It is control wells that one, which is not added with induced polymerization inhibitor or the hole of addition anti-coagulants, and the hole added with induced polymerization inhibitor is detection hole, and each detection unit is to every
Each sample detects one by one respectively in each hole of a sample, and it is small that each detection unit detection obtains blood in sample controls hole, the detection hole
Plate quantity and volume information.Instrument by each blood sample control wells and respectively plus induced polymerization inhibitor detection hole in platelet counts, volume information into
It is horizontal that row relatively obtains each sample platelet aggregation under the effect of different induced polymerization inhibitors.Each unit distribution sequence is according to specimen cup
Direction of transfer is arranged：Agent dispensing unit-detection unit-detection unit.
In the present invention, it is small that the instrument is equipped with the blood that more than one is used to detect blood sample also in Special sample Transmission system
The quantity of plate and the detection unit of volume and an agent dispensing unit.Each detection unit, the sequence of agent dispensing unit according to
Specimen cup transmit sequence be：Detection unit-agent dispensing unit-detection unit-detection unit, each unit respectively with conveyer belt
Upper specimen cup position corresponds to, and when conveyer belt stops, evenly mixing device is also simultaneously stopped work, and each detection unit is simultaneously from position
Specimen cup in draw a certain amount of sample and be detected, and agent dispensing unit is then at the same time to the specimen cup of position
Distribution reagent in position.Each hole all blood samples of specimen cup of the instrument institute adapted carry out one-time detection all before induced polymerization inhibitor is added, with
All each hole all adds in induced polymerization inhibitor afterwards, then each hole blood sample is detected in remaining detection unit again, instrument lures addition
The average value of each hole blood sample platelet counts is as a reference value before poly- agent, each hole add in respective platelet counts after induced polymerization inhibitor,
Volume is compared to each other as being worth after reaction, according to platelet counts, volume change meter in each hole blood sample before and after each induced polymerization inhibitor of addition
It is horizontal to the aggregation capability of specific induced polymerization inhibitor to calculate the sample.The operation principle of detection unit is Kurt testing principle, i.e., outstanding
When floating particle in the electrolytic solution passes through small bore tube with electrolyte, replace the electrolyte of same volume, in the electricity of constant current design
Cause two resistance between electrode inside and outside small bore tube that transient change occurs in road, generate potential pulse, the size and number of pulse signal
It is directly proportional to the size and number of particle.
In the present invention, it is small that the instrument is equipped with the blood that more than one is used to detect blood sample also in Special sample Transmission system
The quantity of plate and the detection unit of volume and an agent dispensing unit.Each detection unit, the sequence of agent dispensing unit according to
Specimen cup transmit sequence be：Detection unit-agent dispensing unit-detection unit, each unit respectively with specimen cup on conveyer belt
Position is corresponding, and when conveyer belt break-off in transmission, evenly mixing device is also simultaneously stopped work, and each detection unit is simultaneously from place
A certain amount of sample is drawn in each hole of specimen cup of position to be detected, and agent dispensing unit is then at the same time to position
Each hole of specimen cup position in distribution reagent.Each hole all blood samples of specimen cup of the instrument institute adapted carry out all before induced polymerization inhibitor is added
One-time detection, then all each hole all adds in induced polymerization inhibitor, then each hole blood sample is detected in remaining detection unit again, instrument
For device using the average value of each hole blood sample platelet counts before addition induced polymerization inhibitor as a reference value, each hole adds in respective blood after induced polymerization inhibitor
Platelet quantity, volume are compared to each other as being worth after reaction, according to add in before and after each induced polymerization inhibitor platelet counts in each hole blood sample,
It is horizontal to the aggregation capability of specific induced polymerization inhibitor that volume change calculates the sample.
In the present invention, the sample transfer system is made of the conveyer belt that a driving motor and driving motor drive,
The equidistant position of conveyer belt is respectively equipped with a suspension specimen cup position, and specimen cup has multiple holes, each each hole difference of specimen cup
The blood sample of packing equivalent is detected, and each sample cup spacing is equal.Conveyer belt at the uniform velocity advances, and advancing under motor drive
Driving motor also causes conveyer belt to move back and forth by moving back and forth in journey, one had in the conveyer belt of instrumental transmission system
A above fixation tooth device, when in conveyer belt moving process and when moving back and forth, the lower part of specimen cup is with fixing tooth percussion
Lower generating state is hindered to change repeatedly, therefore realize mixing to the blood sample in each sample cup, and each in sample transmit process
Sample obtains repeatedly synchronizing mixing, and each hole sample of sample cup is after first time is detected to each Kong Jun at agent dispensing unit
Add in different induced polymerization inhibitors.Conveyer belt according to：Advance and --- retreat --- advance --- pause, the work before then repeating follows
Ring mode, which is realized, to be progressively advanced.
In the present invention, the instrument is equipped with Special sample evenly mixing device and the specimen cup placed on a moving belt is mixed
Even, which is by a motor-driven connecting rod, and connecting rod connection drives multiple mixing bars, and motor drives connecting rod
It moves back and forth, connecting rod drives each mixing bar mutually to touch continuous change with the lower part of specimen cup on conveyer belt respectively by connector
The location status of each sample cup and realize the synchronization mixing to sample in specimen cup on transmission belt.The conveyer belt of instrument according to：Before
It readvances into --- pause ---, the endless form before then repeating is realized gradually to driving front.
In the present invention, the instrument, which has, simultaneously carries out each detection unit complete Quality Control testing process and calibration stream
Journey.It performs Quality Control and the flow of calibration is：Quality-control product target value is pre-entered in instrument controlling unit, and the quality-control product is mixed
It being put into after even in specimen cup and is placed on a designated position on instrument conveyer belt, instrument is detected according to special Quality Control flow,
Only each detection unit detection during being somebody's turn to do, evenly mixing device works in quality-control product transmit process, and reagent point in the process
It does not perform reagent with unit to share out the work, the quality-control product in specimen cup keeps mixing state to detect through each detection unit one by one, instrument
Each detection unit of device has to be automatically corrected by carrying out unification to each detection unit automatically after being detected to identical quality-control product
Function, it is ensured that each detection unit detection performance is consistent.If any detection unit of instrument is to the same quality-control product testing result and target
When being worth more than 5% ＞ of deviation, then instrument is corrected the detection unit deviation project automatically, it is ensured that each detection unit detects matter
The every result obtained is controlled in the range of the target value of Quality Control.
In the present invention, the instrument also has the detection device whether specimen cup is housed to conveyer belt each position, works as sample
When cup position does not load sample, corresponding detection unit is without detecting work.
In the present invention, the disposing way of the instrument sample cup, can also be with conveyer belt direction to be equidirectional with conveyer belt
Vertical distribution.
In the present invention, the specimen cup that the instrument uses has multiple holes, and on two tops of specimen cup, there are one prominent respectively
Axostylus axostyle matched with the suspension position of conveyer belt, for the suspension of specimen cup.
In the present invention, the agent dispensing unit of the instrument and the detection unit of instrument can be separated into two independent instrument
Device.
The invention discloses a kind of high speed platelet function assay instrument and method, the workflow of the instrument is：
Step 1：First blood sample to be checked difference equivalent is added in each hole of Special sample cup, which there are multiple holes, then
Special sample cup is put on the specimen cup position in Special sample Transmission system, there are one prominent respectively on the two sides top of specimen cup
The axostylus axostyle gone out is matched with the suspension position of conveyer belt, for the suspension of specimen cup；
Step 2：Specimen cup is shaken under the action of instrument automatic mixing device and causes blood sample shake mixing in cup, and with
The mobile example cup of conveyer belt is transmitted to first detection unit one by one, first detection unit to blood sample in each hole of each specimen cup into
Row detection obtains volume of platelets and quantity, erythrocyte volume and quantity in original blood sample；
Step 3：Subsequent Special sample Transmission system is moved on is transmitted to agent dispensing unit, reagent unit by specimen cup
Automatically different induced polymerization inhibitors are separately added into each hole of each specimen cup, after adding induced polymerization inhibitor under the action of instrument automatic mixing device
Blood sample shake mixing, and move on；
Step 4：Special sample Transmission system continues blood sample to be transmitted to second detection device, instrument second detection device again
The blood sample is detected respectively to obtain, the platelet counts of blood sample, volume after different induced polymerization inhibitors are added in specimen cup each hole；
Step 5：Specimen cup continuation is forwarded by conveyer belt, and continues the continuous mixing in transmit process, when being transmitted to
When at third detection unit, blood sample of the third detection unit to specimen cup in each hole continues platelet counts, volume detection；
Step 5：Instrument to original blood sample and plus induced polymerization inhibitor aggregation after after blood sample detects respectively, automatically by the first detection
What unit obtained detects acquisition in different times without platelet counts in blood sample before addition induced polymerization inhibitor with other detection units
Addition induced polymerization inhibitor after in blood sample platelet counts result be compared, and calculate in the blood sample under the effect of different induced polymerization inhibitors
The platelet aggregation testing result obtained respectively.The calculation formula of platelet aggregation is as follows：
Maximum platelet aggregation rate=(the original platelet count magnitude-plus induced polymerization inhibitor that first detection unit detection obtains gather
Platelet counts minimum after collection)/first detection unit detection obtain original platelet count magnitude × 100%.
The invention also discloses a kind of high speed platelet function assay instrument and method, the instrument workflow：
Step 1：The specimen cup that Special sample cup difference hole is separately added into quantitative blood sample is loaded in conveyer belt specimen cup position
On, the one of hole of specimen cup is control wells, remaining is detection hole, and the to be checked of equivalent is separately added into control wells and detection hole
Special sample cup is put into Special sample Transmission system by blood sample；
Step 2：When Special sample cup is first transferred to agent dispensing unit by Special sample Transmission system, instrument automatically will
Induced polymerization inhibitor is added in the detection hole of Special sample cup, and quantitative anti-coagulants is added in control wells；
Step 3：Blood sample is shaken into mixing under the action of instrument automatic mixing device, specimen cup is further transmitted to
One detection unit detects the platelet counts in original blood sample and detection hole plus after induced polymerization inhibitor aggregation in blood sample in control wells respectively
And volume；Step 4：When specimen cup is transmitted at second detection unit by Special sample Transmission system, second detection unit pair
The detection hole and control wells blood sample of the specimen cup continue sampling detection respectively；
Step 5：In instrument control wells blood sample and plus induced polymerization inhibitor aggregation after each blood sample after each time point is detected respectively,
Automatically the platelet counts, the body that do not add platelet counts and blood sample after addition induced polymerization inhibitor in induced polymerization inhibitor control blood sample of sample are calculated
Product as a result, and be compared, calculate the blood sample specific induced polymerization inhibitor induction under platelet aggregation rate result.Platelet aggregation
The calculation formula of function is as follows：
Maximum platelet aggregation rate=(platelet count magnitude-plus induced polymerization inhibitor blood sample platelet counts in control wells blood sample)/
Control wells blood sample platelet counts × 100%.
In the present invention, platelet counts and volume that the instrument obtains blood examination according to first detection unit judge
Whether sample is assembled before detection, volume of platelets excessive (＞ 11fl), the platelet count obtained when first time detection
Measure too low (＜ 100 × 109/ L) when, platelet aggregation has occurred before detection for the instrument automatic decision blood sample.Report when pair
The testing result makes prompt.
In the present invention, the instrument conveyer belt 1 or more specimen cup of primary transmission to the corresponding detection unit of quantity, each
Specimen cup is respectively corresponding with a detection unit, i.e., each specimen cup is only by corresponding detection unit detection rather than each
Specimen cup detects one by one through each detection unit, and to each sample cup, each hole sample detects each detection unit respectively, is compared.Transmission
Specimen cup quantity is consistent with detection unit quantity.Each detection unit voluntarily compares meter to the testing result of each sample difference hole blood sample
It is horizontal to the aggregation capability of specific induced polymerization inhibitor to calculate different samples.For example instrument has 4 detection units, conveyer belt once transmits
4 detection cups are respectively to each detection unit, and each detection unit is respectively to platelet count in blood sample in corresponding each hole of detection cup
Amount is detected, and respectively added with the identical blood sample of equivalent in each hole of each sample cup, one of hole is to be added without in the control wells hole
Any reagent, remaining hole are each detection hole of detection hole respectively added with different induced polymerization inhibitors.Each detection unit is by control wells platelet count
Amount is compared with detection hole platelet counts, obtains the blood sample blood platelet to the horizontal result of the aggregation capability of different induced polymerization inhibitors.
Advantageous effect：Instrument of the present invention accelerates the sample speed of service, and Special sample mixing by using Special sample Transmission system
Device can synchronize the different samples on mixing conveyer belt, accelerate sample blending speed；By being added in the different holes of specimen cup
Different induced polymerization inhibitors, after synchronous detection plus different induced polymerization inhibitors, due to extent of platelet aggregation difference, cause blood sample platelet counts and
The difference of volume judges hematoblastic aggregation capability；More than one independent detection unit of apparatus preparation, several test systems are simultaneously
Operation, can synchronize the different samples of detection, and detection speed reaches 40 tests/hour, realizes batch-automated detection, greatly improve
Detection efficiency.Therefore a kind of high speed platelet function assay instrument of the present invention can faster detect hematoblastic aggregation work(
Can, more preferably meet clinical demand.
Description of the drawings
The present invention is done with reference to the accompanying drawings and detailed description and is further illustrated, it is of the invention above-mentioned or
Otherwise advantage will become apparent.
Fig. 1 is that cell distribution sequence of the present invention is the sequence transmitted according to specimen cup：Detection unit-reagent distribution
The structure diagram of unit-detection unit-detection unit.
Fig. 2 is that cell distribution sequence of the present invention is to arrange according to the sequence that specimen cup transmits：Agent dispensing unit-
The structure diagram of detection unit-detection unit-detection unit.
Fig. 3 is that specimen cup disposing way of the present invention is and the equidirectional overlooking the structure diagram of conveyer belt.
Fig. 4 is that specimen cup disposing way of the present invention is to illustrate with the plan structure of conveyer belt direction vertical distribution
Figure.
Fig. 5 is the sample blending apparatus structure schematic diagram that conveyer belt of the present invention moves back and forth.
Fig. 6 is the sample blending apparatus structure schematic diagram of conveyer belt normal operation of the present invention.
Fig. 7-1 is the Special sample cup side view of the present invention for having holes, three holes or four holes, and Fig. 7-2 is the present invention
The Special sample cup vertical view for having holes, three holes or four holes.
Fig. 8 be it is of the present invention there are four, the agent dispensing unit structural representations of three, two or one sample needles
Figure.
Fig. 9 is the structure diagram that detection unit of the present invention has both reagent distribution function.
Specific embodiment
It elaborates below in conjunction with attached drawing to the present invention.
Embodiment 1：
Such as Fig. 1, in the present embodiment, high speed platelet function assay instrument include a Special sample Transmission system P, one with
Upper independent detection unit T, an agent dispensing unit R, each unit distribution sequence are to arrange according to the sequence that specimen cup transmits：Inspection
Survey unit-agent dispensing unit-detection unit-detection unit.Carrying out practically flow is as follows：
First blood sample to be checked difference equivalent is added in Special sample cup C, which is connection cup more than a hole, then will
Special sample cup C, which is put into, to be joined two tops of cup there are one prominent axis respectively on the specimen cup position on Special sample Transmission system P
Bar is matched with the suspension position of conveyor, for the suspension of specimen cup；By specimen cup under the action of instrument automatic mixing device
It shakes so that blood sample shakes mixing in cup, and first detection unit T1 is transmitted to one by one with the mobile example cup of conveyer belt, first
To specimen cup, blood sample is detected platelet counts and volume in acquisition original blood sample to detection unit T1 in each hole；Subsequent special sample
Product Transmission system P is moved on is transmitted to agent dispensing unit R by specimen cup, and agent dispensing unit automatically to specimen cup, divide by each hole
Different induced polymerization inhibitors are not added in, the blood sample after induced polymerization inhibitor will be added to shake mixing, special sample under the action of instrument automatic mixing device
Blood sample is transmitted to second detection device T2 by product Transmission system P again, and instrument second detection device is detected the blood sample respectively
It obtains and platelet counts after different induced polymerization inhibitors in specimen cup, volume is added in each sample cup；Specimen cup continue from conveyor to
Preceding transmission, and continue the continuous mixing in transmit process, when being transmitted at third detection unit T3, third detection unit is to sample
Blood sample in product cup continues platelet counts, volume detection；To original blood sample and after adding induced polymerization inhibitor aggregation, blood sample divides instrument
Not Jian Ce after, automatically by first detection unit obtain without adding in platelet counts and other inspections in blood sample before induced polymerization inhibitor
The platelet counts result that survey unit detects acquisition in different times is compared analysis, and calculate blood platelet in the blood sample
Aggregation capability testing result.
The calculation formula of platelet aggregation is as follows：
Maximum platelet aggregation rate=(the original platelet count magnitude-plus induced polymerization inhibitor that first detection unit detection obtains gather
Platelet counts minimum after collection)/first detection unit detection obtain original platelet count magnitude × 100%.
Embodiment 2：
The present embodiment is distinguished as with embodiment 1, and high speed platelet function assay instrument includes a Special sample transmission system
Unite P, more than one independent detection unit T, an agent dispensing unit R, and each unit distribution sequence is transmitted suitable according to specimen cup
Sequence is：Detection unit-agent dispensing unit-detection unit.
Each unit is corresponding with specimen cup position on conveyer belt respectively, and when conveyer belt stops, evenly mixing device is also simultaneously stopped work
Make, each detection unit is drawn a certain amount of sample from the specimen cup of position simultaneously and is detected, and agent dispensing unit
Then reagent is distributed into the specimen cup position of position at the same time.Each hole all blood samples of specimen cup of the instrument institute adapted all exist
Add and carry out one-time detection before induced polymerization inhibitor, then all each hole all adds in induced polymerization inhibitor, then again in remaining detection unit to each
Hole blood sample is detected, and using the average value of each hole blood sample platelet counts before addition induced polymerization inhibitor as a reference value, each hole adds instrument
Respective platelet counts, volume are compared to each other, as being worth after reaction according to each hole before and after each induced polymerization inhibitor of addition after entering induced polymerization inhibitor
It is horizontal to the aggregation capability of specific induced polymerization inhibitor to calculate the sample for platelet counts, volume change in blood sample.
Embodiment 3：
The present embodiment is distinguished as with embodiment 1, and high speed platelet function assay instrument includes a Special sample transmission system
Unite P, more than one independent detection unit T, an agent dispensing unit R, and each unit distribution sequence is transmitted suitable according to specimen cup
Sequence is：Agent dispensing unit-detection unit-detection unit-detection unit.Carrying out practically flow is as follows：
Such as Fig. 2, Special sample cup C differences hole is first divided into control wells and detection hole, there are one control wells, remaining is inspection
Gaging hole, is separately added into the blood sample to be checked of equivalent in control wells and detection hole, and EDTA anti-coagulants can be added in control wells
Or anti-coagulants is not added with, only blood sample to be checked is added to be not added with anti-coagulants in detection hole, Special sample cup C then is put into Special sample passes
In defeated system；When Special sample cup is first transferred to agent dispensing unit R by Special sample Transmission system P, instrument will lure automatically
Poly- agent is added in the detection hole in Special sample cup, and induced polymerization inhibitor is added without in control wells；In instrument automatic mixing device
Blood sample is shaken into mixing under effect, specimen cup is further transmitted to first detection unit T1, detects original blood in control wells
In sample and detection hole plus induced polymerization inhibitor aggregation after blood sample platelet counts and volume, erythrocyte number and volume；Work as Special sample
When specimen cup C is transmitted at second detection unit T2 by Transmission system P, second detection unit is to the detection hole of the specimen cup and right
Detection is sampled according to hole；When specimen cup C is transmitted at third detection unit T3 by Special sample Transmission system P, third inspection
Unit is surveyed to be detected blood sample sampling in the detection hole and control wells of the specimen cup；Instrument is to original blood sample and induced polymerization inhibitor is added to gather
For each blood sample after each time point is detected respectively, automatic calculating each sample does not add blood platelet in induced polymerization inhibitor original blood sample after collection
Number average, and choose the platelet counts result that detection of different time (different detection units) obtains and be compared point
Analysis, and choose detection acquisition platelet counts minimum result and be compared simultaneously with original blood sample blood platelet average detection result
Maximum platelet aggregation rate of the blood sample under the induction of specific induced polymerization inhibitor is calculated, calculation formula is as follows：
Maximum platelet aggregation rate=(platelet counts minimum after original platelet count magnitude-plus induced polymerization inhibitor aggregation)/
Original platelet counts × 100%.
Embodiment 4：
The present embodiment is distinguished as with embodiment 1, and high speed platelet function assay instrument includes a Special sample transmission system
Unite P, more than one independent detection unit T, an agent dispensing unit R, and each unit distribution sequence is to be according to specimen cup transmission
Sequentially：Agent dispensing unit-detection unit-detection unit.
Specimen cup selected by instrument has multiple holes, and each each hole of specimen cup is mutual indepedent and is respectively previously charged into equivalent
Identical blood sample, one of hole is not added with induced polymerization inhibitor as control wells, and the hole added with induced polymerization inhibitor is detection hole, and each detection unit is to every
Each sample detects one by one respectively in each hole of a sample, and it is small that each detection unit detection obtains blood in sample controls hole, the detection hole
Plate quantity and volume information.Instrument by each blood sample control wells and respectively plus induced polymerization inhibitor detection hole in platelet counts, volume information into
It is horizontal that row relatively obtains each sample platelet aggregation under the effect of different induced polymerization inhibitors.
Embodiment 5：
The present embodiment is distinguished as with embodiment 1, and the sample blending mode of high speed platelet function assay instrument is conveyer belt
Under fixation tooth it is fixed, conveyer belt moves reciprocatingly, the sample blending device N1 in fixed tooth schematic diagram such as Fig. 1, specifically
Mixing method is as follows：
Such as Fig. 5, the baffle on Special sample evenly mixing device N1 is fixed, and conveyer belt moves back and forth, and Special sample cup C exists
Conveyer belt also moves reciprocatingly under driving, and when conveyer belt is run to the left, the fixation tooth on evenly mixing device is encountered in specimen cup bottom,
Specimen cup C is tilted to the left, and across fixed tooth, is then transported on band and is run to the right, when evenly mixing device is encountered in specimen cup bottom again
During fixed tooth, specimen cup C is tilted to the right, and across fixed tooth.Conveyer belt drives Special sample cup to move back and forth, the above process
It is repeated several times, achievees the purpose that sample blending.
Embodiment 6：
The present embodiment is distinguished as with embodiment 1, and the sample blending mode of high speed platelet function assay instrument is conveyer belt
Sample blending device in normal operation, and being moved reciprocatingly under conveyer belt by motor-driven connecting rod, connecting rod schematic diagram such as Fig. 2
N2, specific mixing method are as follows：
Such as Fig. 6, conveyer belt normally advances, and separately sets the Special sample evenly mixing device N2 of a multi link, reciprocal by it
The specimen cup C of operation and conveyer belt, which is mutually touched, constantly to be changed the location status of each sample cup and realizes to sample in specimen cup
Synchronous mixing.When conveyer belt is run to the left, Special sample evenly mixing device N2 is also run to the left, and evenly mixing device forward speed ratio
Conveyer belt is slightly fast, and the catch bar of evenly mixing device catch up with specimen cup C from afterwards, when specimen cup encounters the catch bar of right evenly mixing device
When, specimen cup bottom is toward Left-Tilt, and catch bar, to the left across specimen cup, subsequent evenly mixing device moves right, specimen cup bottom
The catch bar positioned at the left side is encountered in portion, and specimen cup bottom is tilted toward the right, and catch bar is to the right across specimen cup, above process weight
Plural time, achieve the purpose that sample blending.
Embodiment 7：
The present embodiment is distinguished as with embodiment 1, and the disposing way of high speed platelet function assay instrument specimen cup is and biography
It send with equidirectional.
Such as Fig. 3, for the detection unit T of instrument there are one more than, the specimen cup C selected by instrument has multiple holes, each sample
Each hole of cup is respectively previously charged into the identical blood sample of equivalent, wherein a hole for being not added with induced polymerization inhibitor is control wells, added with induced polymerization inhibitor
Hole be detection hole.Specimen cup is put on Special sample Transmission system P, and disposing way is equidirectional with conveyer belt.Each inspection
It surveys unit to detect each sample in each hole of every a sample one by one respectively, each detection unit detection obtains the sample controls hole, inspection
Platelet counts and volume information in gaging hole.Instrument by each blood sample control wells and respectively plus induced polymerization inhibitor detection hole in platelet counts,
Volume information is compared to obtain each sample platelet aggregation level under the effect of different induced polymerization inhibitors.Each unit distribution sequence
According to specimen cup transmit sequence be：Agent dispensing unit R- detection unit T1- detection units T2.
Embodiment 8：
The present embodiment is distinguished as with embodiment 1, and the disposing way of high speed platelet function assay instrument specimen cup is and biography
Send band direction vertical distribution.
Such as Fig. 4, for the detection unit T of instrument there are one more than, the specimen cup C selected by instrument has multiple holes, each sample
Each hole of cup is respectively previously charged into the identical blood sample of equivalent, wherein a hole for being not added with induced polymerization inhibitor is control wells, added with induced polymerization inhibitor
Hole be detection hole.Specimen cup is put on Special sample Transmission system P, and disposing way divides to be vertical with conveyer belt direction
Cloth.Each detection unit detects each sample in each hole of every a sample one by one respectively, and each detection unit detection obtains the sample pair
According to platelet counts and volume information in hole, detection hole.Blood is small by each blood sample control wells and respectively plus in induced polymerization inhibitor detection hole for instrument
Plate quantity, volume information are compared to obtain each sample platelet aggregation level under the effect of different induced polymerization inhibitors.Each unit
Distribution sequence is according to the sequence that specimen cup transmits：Agent dispensing unit R- detection unit T1- detection units T2.
Embodiment 9：
The present embodiment is distinguished as with embodiment 1, the sample used such as Fig. 7-1, Fig. 7-2, high speed platelet function assay instrument
Product cup has multiple holes, can use there are two hole specimen cup, can also use there are three hole or four holes specimen cup.In sample
Axostylus axostyle of two tops of product cup respectively there are one protrusion to be matched with the suspension position of conveyer belt, for the suspension of specimen cup.
Embodiment 10：
The present embodiment is distinguished as with embodiment 1, as Fig. 8, instrument reagent allocation unit R contain reagent bottle B and sample needle
G.Reagent bottle quantity is identical with sample needle quantity, and can be identical with the quantity of sample cup aperture, can there are one, two or three
It is a.When there are two or during three sample needles, during agent dispensing unit distribution reagent can simultaneously in specimen cup difference hole simultaneously
Add in different induced polymerization inhibitors or anti-coagulants.
Embodiment 11：
The present embodiment is distinguished as with embodiment 1, the conveyer belt once transmission 2 or more of high speed platelet function assay instrument
Specimen cup is to the corresponding detection unit of quantity, and each specimen cup is only in the detection of a corresponding detection unit rather than each sample
Cup detects one by one through each detection unit.
Specific implementation method can such as Fig. 2, in advance in specimen cup difference Kong Zhongjia induced polymerization inhibitors or anti-coagulants before instrument detection,
Then each sample cup is assigned at each detection unit and is detected, each detection unit to each sample difference cup testing result voluntarily
Compare and calculate aggregation capability level of the different samples to specific induced polymerization inhibitor.
Specific implementation method can not also need to individual agent dispensing unit such as Fig. 9, instrument, and detection unit has both reagent
Different specimen cups are assigned to each detection unit by distribution function, instrument, and each detection unit presses the sequence of detection-reagent adding-detection
Detect respectively plus induced polymerization inhibitor before and after platelet counts and volume, each detection unit to each sample difference cup testing result voluntarily
Compare and calculate aggregation capability level of the different samples to specific induced polymerization inhibitor.
Embodiment 12：
The present embodiment is distinguished as with embodiment 1, high speed platelet function assay instrument have simultaneously to each detection unit into
The complete Quality Control testing process of row and calibration flow.It performs Quality Control and the flow of calibration is：In instrument controlling unit in advance
Quality-control product target value is inputted, and a designated position on instrument conveyer belt is placed on by being put into specimen cup after the quality-control product mixing,
Instrument is detected according to special Quality Control flow, and only each detection unit detection, evenly mixing device are transmitted in quality-control product in this process
It works in the process, and agent dispensing unit does not perform reagent and shares out the work in the process, the quality-control product in specimen cup keeps mixed
Even state detects one by one through each detection unit, each detection unit of instrument have by after being detected to identical quality-control product automatically to each
Detection unit carries out the zero offset capability of unification, it is ensured that each detection unit detection performance is consistent.If any detection of instrument is single
When member is to the same quality-control product testing result and more than 5% ＞ of target value deviation, then instrument is automatic to the detection unit deviation project
It is corrected, it is ensured that every result that each detection unit detection Quality Control obtains is in the range of the target value of Quality Control.
Embodiment 13：
The present embodiment is distinguished as with embodiment 1, high speed platelet function assay instrument also have be to conveyer belt each position
The no detection device equipped with specimen cup, when specimen cup position does not load sample, corresponding detection unit is without detection.
Embodiment 14：
The present embodiment is distinguished as with embodiment 1, and high speed platelet function assay instrument is according to first detection unit to blood sample
It detects the platelet counts obtained and whether volume judgement sample is assembled before detection, the blood obtained when first time detection
Platelet volume excessive (＞ 11fl), the too low (＜ 100 × 10 of platelet counts9/ L) when, the instrument automatic decision blood sample is detecting
It is preceding that platelet aggregation has occurred.Prompt is made to the testing result in report.
The present invention provides a kind of high speed platelet function assay instrument and method, implement the technical solution method and
There are many approach, and the above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.All undefined components in this embodiment can be implemented in the prior art.
Claims (10)
1. a kind of high speed platelet function assay instrument and method, which is characterized in that detecting system includes a Special sample and transmits
System, more than one independent detection unit and control unit；The Special sample Transmission system is used to transmit multiple samples simultaneously
Cup passes through each detection unit；The detection unit is used to draw the sample when specimen cup stays in the detection unit corresponding position
Blood sample in each hole of cup, while the platelet counts in each blood sample, volume are detected respectively；Each detection unit obtains identical blood sample warp
Induced polymerization inhibitor processing and change without the platelet counts that induced polymerization inhibitor is handled, by comparing the blood platelet after identical blood sample different disposal
The variation of quantity is obtained to the horizontal result of the platelet function of blood sample.
2. a kind of high speed platelet function assay instrument according to claim 1 and method, which is characterized in that the instrument also has
There are one agent dispensing units；Specimen cup selected by instrument includes a control wells and more than one detection hole, the control
Induced polymerization inhibitor is not added in hole, and detection hole adds induced polymerization inhibitor, and each each hole of specimen cup is mutual indepedent and is previously charged into equivalent respectively
Identical blood sample；Each detection unit detects each sample in each hole of every a sample one by one respectively, and each detection unit detection is somebody's turn to do
Platelet counts and volume information in sample controls hole, detection hole；Instrument is by platelet count in each blood sample control wells and detection hole
Amount is compared, and it is horizontal to obtain each sample platelet aggregation under the effect of different induced polymerization inhibitors；Each unit distribution sequence foundation
Specimen cup pass order：Agent dispensing unit-detection unit-detection unit.
3. a kind of high speed platelet function assay instrument according to claim 1 and method, which is characterized in that the instrument also exists
Special sample Transmission system is equipped with more than one for detecting the detection unit and one of hematoblastic quantity and volume in blood sample
A agent dispensing unit；Each detection unit, the sequence of agent dispensing unit along transmission carry sample moving direction be sequentially distributed for：Inspection
Unit-agent dispensing unit-detection unit-detection unit is surveyed, each unit is corresponding with specimen cup position on conveyer belt respectively, is working
In when conveyer belt suspends, evenly mixing device also simultaneously break-off, and each detection unit simultaneously from the specimen cup of position
It draws a certain amount of sample to be detected, and agent dispensing unit is then at the same time into the specimen cup position detection hole of position
Distribute reagent.Each hole all blood samples of specimen cup of the instrument institute adapted carry out one-time detection all before induced polymerization inhibitor is added, then complete
Each hole in portion all adds in induced polymerization inhibitor, then each hole blood sample is detected in remaining detection unit again, instrument will add in induced polymerization inhibitor
Preceding each hole blood sample platelet counts are as a reference value, and respective platelet counts, volume are as reaction after each hole adds in induced polymerization inhibitor
After be worth, the two is compared to each other, and according to adding in before and after each induced polymerization inhibitor, platelet counts, volume change calculate the sample in each hole blood sample
Product are horizontal to the aggregation capability of specific induced polymerization inhibitor.
4. a kind of high speed platelet function assay instrument according to claim 1 and method, which is characterized in that the special sample
Product Transmission system is made of the conveyer belt that a driving motor and driving motor drive, and is respectively equipped in the equidistant position of conveyer belt
One suspension specimen cup position, specimen cup have multiple holes, and the blood sample that each each hole of specimen cup dispenses equivalent respectively is detected, respectively
Specimen cup spacing is equal.Conveyer belt motor drive under at the uniform velocity advance, and during advance driving motor also by back and forth transporting
It is dynamic that conveyer belt is caused to move back and forth, in the more than one fixed tooth device that the conveyer belt of instrumental transmission system has, work as biography
When sending in band moving process and moving back and forth, the lower part of specimen cup changes repeatedly with generating state under fixed tooth percussion obstruction,
Therefore mixing is realized to the blood sample in each sample cup, and obtains repeatedly synchronizing mixing per a sample in sample transmit process,
Each hole sample of sample cup adds in different induced polymerization inhibitors at agent dispensing unit after first time is detected to each hole.Conveyer belt is pressed
According to：Movement of advancing --- retreats --- advance --- pause, the working cycles mode before then repeating, which is realized, to be progressively advanced.
5. a kind of high speed platelet function assay instrument according to claim 1 and method, which is characterized in that the instrument has
Complete Quality Control and calibration flow are carried out to each detection unit simultaneously；Quality-control product target value is pre-entered in instrument controlling unit,
And will be put into specimen cup after the quality-control product mixing, the specimen cup position of instrument conveyer belt is placed on, instrument is according to special Quality Control
Flow detects, in this process only each detection unit detection, and evenly mixing device works in quality-control product transmit process, and mistake herein
Agent dispensing unit does not perform reagent and shares out the work in journey, and the quality-control product in specimen cup keeps mixing state, one by one through each detection
Unit detects, after each detection unit of instrument has by being detected to identical quality-control product, when the detection that any detection unit obtains
As a result when and target value deviation is more than 5%, instrument automatically carries out each detection unit the zero offset capability of unification, it is ensured that each inspection
It is consistent to survey unit detection performance.
6. a kind of high speed platelet function assay instrument according to claim 1 and method, which is characterized in that the instrument also has
There is the detection device whether specimen cup is housed to conveyer belt each position, which does not load specimen cup
When, corresponding detection unit is without detecting work.
7. a kind of high speed platelet function assay instrument and method according to claim 1 or 3, which is characterized in that the instrument
Workflow be：
Step 1：First blood sample to be checked difference equivalent is added in each hole of Special sample cup, which there are multiple holes, then will be special
It is put on the specimen cup position in Special sample Transmission system with specimen cup, there are one protrusions respectively on the two sides top of specimen cup
Axostylus axostyle is matched with the suspension position of conveyer belt, for the suspension of specimen cup；
Step 2：Specimen cup is shaken so that blood sample shakes mixing, and with transmission in cup under the action of instrument automatic mixing device
The mobile example cup of band is transmitted to first detection unit one by one, and first detection unit examines blood sample in each hole of each specimen cup
It surveys and obtains volume of platelets and quantity, erythrocyte volume and quantity in original blood sample；
Step 3：Subsequent Special sample Transmission system is moved on is transmitted to agent dispensing unit by specimen cup, and reagent unit is automatic
Different induced polymerization inhibitors are separately added into each hole of each specimen cup, by the blood after adding induced polymerization inhibitor under the action of instrument automatic mixing device
Sample shakes mixing, and moves on；
Step 4：Special sample Transmission system continues blood sample to be transmitted to second detection device again, and instrument second detection device is to this
Blood sample is detected respectively, obtains and the platelet counts of blood sample, volume after different induced polymerization inhibitors are added in specimen cup each hole；
Step 5：Specimen cup continuation is forwarded by conveyer belt, and continues the continuous mixing in transmit process, when being transmitted to third
When at detection unit, blood sample of the third detection unit to specimen cup in each hole continues platelet counts, volume detection；
Step 6：Instrument to original blood sample and plus induced polymerization inhibitor aggregation after after blood sample detects respectively, automatically by first detection unit
What is obtained detects adding for acquisition in different times without platelet counts in blood sample before addition induced polymerization inhibitor with other detection units
Enter after induced polymerization inhibitor that platelet counts result is compared in blood sample, and calculate in the blood sample under the effect of different induced polymerization inhibitors respectively
The platelet aggregation testing result of acquisition.
A kind of 8. high speed platelet function assay method according to claim 1 or 2, which is characterized in that the work of the instrument
It is as flow：
Step 1：The specimen cup that Special sample cup difference hole is separately added into quantitative blood sample is loaded on conveyer belt specimen cup position, sample
The one of hole of product cup is control wells, remaining is detection hole, and the blood sample to be checked of equivalent is separately added into control wells and detection hole,
Special sample cup is put into Special sample Transmission system；
Step 2：Special sample cup is first transferred to agent dispensing unit by Special sample Transmission system first, and instrument will lure automatically
Poly- agent is added in the detection hole of Special sample cup, and quantitative anti-coagulants is added in control wells；
Step 3：Blood sample is shaken into mixing under the action of instrument automatic mixing device, specimen cup is further transmitted to the first inspection
Unit is surveyed, detects platelet counts and body in original blood sample and detection hole plus after induced polymerization inhibitor aggregation in blood sample in control wells respectively
Product；
Step 4：When specimen cup is transmitted at second detection unit by Special sample Transmission system, second detection unit is to the sample
The detection hole and control wells blood sample of product cup continue sampling detection respectively；
Step 5：In instrument control wells blood sample and plus induced polymerization inhibitor aggregation after each blood sample after each time point is detected respectively, automatically
Calculate the platelet counts, the volume knot that do not add platelet counts and blood sample after addition induced polymerization inhibitor in induced polymerization inhibitor control blood sample of sample
Fruit, and be compared, calculate platelet aggregation rate result of the blood sample under the induction of specific induced polymerization inhibitor.
9. a kind of high speed platelet function assay instrument according to claim 1 and method, which is characterized in that the instrument according to
Whether the platelet counts and volume judgement sample that first detection unit obtains blood examination are assembled before detection, when
The volume of platelets obtained during one-time detection is more than 11fl, and quantity is less than 100 × 109During/L, in the instrument automatic decision blood sample
Blood platelet has been assembled before detection.
10. a kind of high speed platelet function assay instrument according to claim 1 and method, which is characterized in that the instrument passes
Band is sent once to transmit more than one specimen cup to the corresponding detection unit of quantity, each specimen cup respectively with a detection unit pair
Should, i.e., each specimen cup is only examined through each detection unit one by one by the detection of a corresponding detection unit rather than each sample cup
It surveys, each detection unit is to being not added with sample point in the control wells of induced polymerization inhibitor and each detection hole added with different induced polymerization inhibitors in each sample cup
It does not detect, the control wells blood sample platelet counts of each sample is compared respectively with the platelet counts in detection cup blood sample,
Calculate the platelet aggregation rate of the blood sample；To different specimen cups, each hole blood sample is detected each detection unit respectively, is voluntarily compared
Aggregation capability of the different samples under the effect of specific induced polymerization inhibitor is calculated more afterwards.
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| WO2021238109A1 (en) * | 2020-05-25 | 2021-12-02 | 北京中勤世帝生物技术有限公司 | Fixed-rate test method for blood coagulation routine test |
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| WO2021238109A1 (en) * | 2020-05-25 | 2021-12-02 | 北京中勤世帝生物技术有限公司 | Fixed-rate test method for blood coagulation routine test |
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