CN108195956A - The assay method of food vitamins E contents - Google Patents
The assay method of food vitamins E contents Download PDFInfo
- Publication number
- CN108195956A CN108195956A CN201711349912.XA CN201711349912A CN108195956A CN 108195956 A CN108195956 A CN 108195956A CN 201711349912 A CN201711349912 A CN 201711349912A CN 108195956 A CN108195956 A CN 108195956A
- Authority
- CN
- China
- Prior art keywords
- assay method
- contents
- methanol
- vitamin
- test solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to a kind of assay methods of food vitamins E contents, include the following steps：(1) preparation of test solution：Sample to be tested is taken to be dissolved in methanol, after vortex mixed, ultrasonic extraction and centrifugation, the supernatant liquor filtering after centrifugation is taken, obtains test solution；(2) Ultra Performance Liquid Chromatography instrument measures：The test solution is taken, the content of vitamin E in the test solution is measured using Ultra Performance Liquid Chromatography instrument, the condition that the Ultra Performance Liquid Chromatography instrument measures includes：Chromatographic column：C18 (150mm × 4.6mm, 5 μm)；Mobile phase：Methanol；Flow velocity：0.6~1.1mL/min.The detection method of the present invention has the advantages of accuracy is high, favorable reproducibility.
Description
Technical field
The present invention relates to detection field, more particularly to the assay method of food vitamins E contents.
Background technology
Vitamin E (vitamin E) is a kind of antioxidant that can offset oxidation, is tocopherol
The general name of (tocopherol, T) and tocotrienol (Tocotrienol, T-3), are a kind of liposoluble vitamins, are not only
Important composition ingredient on cell membrane is also the primary anti-oxidant on cell membrane.Vitamin E is widely present in animals and plants food
In, the vitamin E in animal food is based on da types, and the content of vitamin E in vegetable oil is more and content and linoleic acid etc.
Polyenoic fatty acid content is parallel.
Early in the 1920s, vitamin E just by it is found that.It is defended according to China Preventive Medicial Science Institute's nutrition with food
Raw research institute Yang Xiaoguang researcher introduces：Nineteen twenty-two foreign countries expert has found a kind of fat-soluble factor diet to the normal numerous of big white mouse
It is essential to educate, and the nineteen twenty-four fat-soluble factor diet is just named as vitamin E, and the crystalline solid of vitamin E in 1936 is divided
It separates out and, the artificial synthesized vitamin E of Swiss chemists in 1938.In zoopery later, scientists are found, little Bai
Mouse will appear the heart, liver and muscle deterioration and barren symptom if the E that is deficient in vitamin；If big white mouse is deficient in vitamin
E then male permanent infertility, female cannot cherish mature fetus, at the same also liver degenerate, the symptom of myocardial abnormality；Monkey lacks dimension
Raw element E just will appear anaemia, infertility, myocardial abnormality；The eighties, medical experts have found, if the mankind have lacked vitamin E
Genetic disease and metabolic disease can then be caused.With going deep into for research, medical expert recognizes vitamin E in the prevention heart again
Cranial vascular disease, tumour, diabetes and other complication, central nervous system disease, motor system disease and skin disease
Etc. there is extensive effect.
At present, the main source of vitamin E is vegetable oil and embryo oil, such as wheat germ oil, maize germ oil, cottonseed oil, flower
Oil generation and sesame oil etc., the composition and content of vitamin E can as an important evaluation index of edible vegetable oil and fat quality,
And vitamin E is common antioxidant in food, vitamin E universally present in food, in view of vitamin have it is high
Nutritive value, the vitamin E contained in food, which is detected, to be necessary.The analysis method of vitamin E has very much,
Including colorimetric method, spectroscopic methodology, paper chromatography, column chromatography, thin-layered chromatography, gas chromatography and high performance liquid chromatography etc..
The detection method of international vitamin E is mostly using liquid chromatography, China current standard GB/T 5009.82-2003《Food
The measure of vitamin A and vitamin E in product》、GB 5413.9-2010《The survey of vitamin A. D. E in infant food and dairy products
It is fixed》And professional standard NY/T 1598-2008《The measure high performance liquid chromatography of vitamin e ingredient and content in edible oil》
It is to use liquid chromatography Deng.However tradition measures the methods of food vitamins E contents that there are sample volume is big, saponification
Return time is long, pre-treatment is troublesome and the shortcomings such as accuracy is low.
Invention content
Based on this, the present invention provides a kind of assay method of food vitamins E contents, which has operation
The advantages of simple and accuracy is high.
Specific technical solution is as follows：
A kind of assay method of food vitamins E contents, includes the following steps：
(1) preparation of test solution：
Sample to be tested is taken to be dissolved in methanol, after vortex mixed, ultrasonic extraction and centrifugation, takes the supernatant liquor after centrifugation
Filtering, obtains test solution；
(2) Ultra Performance Liquid Chromatography instrument measures：
The test solution is taken, containing for vitamin E in the test solution is measured using Ultra Performance Liquid Chromatography instrument
Amount, the condition that the Ultra Performance Liquid Chromatography instrument measures include：
Chromatographic column：C18 (150mm × 4.6mm, 5 μm)；
Mobile phase：Methanol；Flow velocity：0.6~1.2mL/min.
In wherein some embodiments, the condition that the Ultra Performance Liquid Chromatography instrument measures further includes：
Column temperature：25-35；
Sample size：9-11.
In wherein some embodiments, the condition that the Ultra Performance Liquid Chromatography instrument measures includes：
Chromatographic column：C18 (150mm × 4.6mm, 5 μm)；
Mobile phase：Methanol；Flow velocity：1.0mL/min；
Column temperature：30℃；
Sample size：10μL.
In wherein some embodiments, the proportioning of the sample to be tested and the methanol is 0.15-0.25g：10mL.
In wherein some embodiments, the proportioning of the sample to be tested and the methanol is 0.2g：10mL.
In wherein some embodiments, the time of the ultrasonic extraction is 14-30min.
In wherein some embodiments, the time of the ultrasonic extraction is 14-16min.
In wherein some embodiments, the time of the vortex mixed is 3-5min.
In wherein some embodiments, the time of the centrifugation is 1-2min, and the rotating speed of the centrifugation is 3.0 × 103-
4.0×103rpm。
In wherein some embodiments, the filtering uses the filter membrane that aperture is 0.45 μm.
The assay method of the food vitamins E contents of the present invention has the following advantages and beneficial effect：
The assay method of traditional food vitamins E contents is usually required sample to be tested by complicated pre-treatment
It is detected again after journey, than being detected again if desired for sample to be tested is obtained test solution after saponification and complicated extraction.
The present inventor has found that the process of traditional saponification and extraction can cause vitamin E in sample by a large amount of performing creative labours
A large amount of losses, cause testing result accuracy be deteriorated.Further by lot of experiments to the pre-treating method of sample into
Row optimization, when the present invention prepares test solution, simplifies the extraction operation of target substance while reducing saponification operation, with
The step of Extraction solvent of the methanol as vitamin E, cooperation vortex mixed, ultrasonic extraction and centrifugation, can efficiently, fully
Vitamin E from sample to be tested is extracted, reduces loss of the vitamin E in pretreatment process, and can reduce
Other impurity in extracting solution so as to reduce interference of the impurity to testing result, substantially increase the accuracy of testing result.
In addition, the test solution of the present invention is detected under the conditions of the ultra performance liquid chromatography of optimization, this is further increased
The accuracy of invention food vitamins E content assaying methods.
Further, the relevant parameter measured to the preparation condition of test solution and ultra performance liquid chromatography carries out excellent
Change, further improve the accuracy of testing result.
Specific embodiment
The present invention is described in further detail below in conjunction with specific embodiment.
Sample to be tested in following embodiment is Australia's fitness board wheat-germ oil vitamin E soft capsule, in the product to be tested
Content of vitamin E is 42ppm.
Embodiment 1
The preparation of reference substance solution：
Vitamin E standard items are taken, add in the reference substance solution that a concentration of 1.0261mg/ml is made in methanol；
The preparation of test solution：
It accurately weighs 0.2g samples to be tested to be placed in the centrifuge tube of 10mL, adding in methanol dissolves sample to be tested, is vortexed
4min makes to be uniformly mixed, ultrasonic extraction 15min, then add methanol constant volume to 10mL, then rotating speed be 3.5 × 103Under conditions of rpm
1min is centrifuged, the supernatant liquid filtering after centrifugation (filter sizes are 0.45 μm) is taken, obtains test solution.
Ultra Performance Liquid Chromatography instrument measures：Reference substance solution and test solution is taken to distinguish sample introduction, according to following ultra high efficiency
Chromatographic condition is detected, and obtains chromatogram, and content of vitamin E in test solution is calculated to obtain with external standard method：
Chromatographic column：C18 (150mm × 4.6mm, 5 μm)；
Mobile phase：Methanol；Flow velocity：1.0mL/min；
Column temperature：30℃；
Sample size：10μL；
Detector：UV detector；
Detection wavelength：284nm.
According to the method for embodiment 1,3 parts of samples to be tested is taken to be tested, calculate the average value and RSD of test result.
Embodiment 2
(1) influence of the ultrasonic time to the measurement result of food vitamins E contents
Using the assay method of embodiment 1, respectively using ultrasonic time as 0min, 10min, 15min, 20min and 30min
It is tested, test result such as table 1：
The measurement result of the different ultrasonic time of table 1
As a result：As seen from the above table, when preparing test solution, if not being ultrasonically treated, vitamin E in test result
Content is 10.12ppm, RSD 3.13%, measures the content of vitamin E and can be substantially reduced；Ultrasonic time is 10min, test knot
Vitamin content in fruit is 20.14ppm, RSD 3.32%, and the content for measuring vitamin E is also relatively low；Ultrasonic time is 15-
During 30min, the vitamin content in test result is all in 42ppm or so, and RSD shows that ultrasonic time is no more than 0.6%
During 15-30min, the accuracy of measurement result and reproducibility all improve, and illustrate that vitamin E can relatively adequately on this condition
It is extracted and disperses in methyl alcohol evenly.Consider detection time, most preferably ultrasonic time is 15min.
(2) influence of the flowing phase composition to the measurement result of food vitamins E contents
Using the assay method of embodiment 1, respectively with acetonitrile：Water=80:20, methanol：Water=60：40th, methanol：Water=
80:20 and methanol eluted as mobile phase, test result such as table 2：
The measurement result of the different mobile phase of table 2
| Mobile phase | Vitamin E (ppm) | Peak type |
| Acetonitrile：Water=80:20 | - | It is very bad |
| Methanol：Water=60:40 | - | It is very bad |
| Methanol：Water=80:20 | - | It is bad |
| Methanol | 41.16 | Very well |
As a result：As seen from the above table, it is that mobile phase measures the content of vitamin E as 41.16ppm using methanol, peak type is fine,
The peak type of chromatogram that his three kinds of mobile phases obtain is bad, can not determine the content of vitamin E, therefore be surveyed by mobile phase of methanol
The best results and peak type of vitamin E are most preferable.
(3) influence of the flow rate of mobile phase to the measurement result of food vitamins E contents
Using the assay method of embodiment 1, respectively using flow rate of mobile phase as 0.6mL/min, 0.8mL/min, 1.0mL/min
And 1.2mL/min is tested, test result such as table 3：
The measurement result of the different flow rate of mobile phase of table 3
As a result：When testing the content of vitamin E as mobile phase using methanol, appearance time is ideal for 5-10min
Appearance time, when flow velocity is 0.6mL/min, appearance time is 18min or so, and appearance time is too long；Flow velocity is 1.2mL/min
When, appearance time is 4min or so, and appearance time is shorter, and testing result is easily influenced by other impurities in solvent, can shadow
The accuracy of testing result is rung, therefore the flow velocity of methanol is can obtain ideal appearance time during 1.0mL/min.
Comparative example 1
This comparative example uses Ministry of Health of the People's Republic of China GB 5413.9-2010《Life is tieed up in infant food and dairy products
The measure of plain A, D, E》The measure scheme of middle vitamin E, it is specific as follows：
The preparation of reference substance solution：
The preparation of reference substance solution is the same as embodiment 1.
The preparation of test solution：
50g samples to be tested directly are weighed in 250mL triangular flasks, add in the ascorbic ethanol solutions of 100mL (15g/L),
After abundant mixing, 25mL potassium hydroxide aqueous solutions (1.25g/mL) are added, is uniformly mixed, magnetic agitation is put into triangular flask
Stick is simultaneously filled with nitrogen to discharge air, covers rubber plug；
The another water for taking beaker (1000mL) and 300mL is added in into beaker, beaker is placed in constant temperature blender with magnetic force, is burnt
Water temperature in cup is controlled at 53 DEG C ± 2 DEG C, the triangular flask for covering rubber plug is put into beaker, saponification under conditions of magnetic agitation
After handling 45min, triangular flask is taken out from beaker, is cooled to room temperature at once, obtains saponification liquor；
The saponification liquor in triangular flask is all transferred in 500mL separatory funnels with a small amount of water, adds in 100mL petroleum ethers,
It gently shakes, bottle stopper is covered after exhaust, vibrate stratification after about 10min at room temperature, the water phase after stratification is transferred to separately
In an outer separatory funnel (500mL), carry out extracting for second as stated above；Merge extraction for the first time and second of extraction
Ether liquid is washed with water to weakly acidic pH, is washed to neutral ether liquid and is poured into 500mL by anhydrous sodium sulfate filtering means dehydration, then by filtrate
In round-bottomed flask, in Rotary Evaporators (temperature：40 DEG C ± 2 DEG C, nitrogen) on remove petroleum ether, obtain residue；
It will be transferred in 10mL volumetric flasks after residue petroleum ether dissolution, it is molten accurately to pipette 2.0mL from volumetric flask for constant volume
The petroleum ether solution that solution has residue is put into test tube, test tube is placed in 40 DEG C ± 2 DEG C of nitrogen evaporator, by the petroleum ether in test tube
Drying, then add 5.0mL methanol into test tube, dissolved residue is vibrated, then test tube is centrifuged with the speed for being not less than 5000 revs/min
10min, take out stand to room temperature to get.
Liquid-phase chromatographic analysis：Test solution and reference substance solution is taken to be tested according to following liquid phase chromatogram condition：
Chromatographic column：C18 columns；
Mobile phase：Methanol；Flow velocity：1.0mL/min；
Detection wavelength：294nm.
According to the method described above, it is parallel to take 5 parts of samples to be tested, as a result such as following table：
As a result：As seen from the above table, it is 26.43ppm according to the content of the GB 5413.9-2010 vitamin Es measured, RSD is
11.51%, main cause is that the sampling amount of GB 5413.9-2010 is big, and sample processing time is long, cumbersome, and sample is extracting
Loss is more in the process, low so as to cause the result precision and reproducibility of test.
Comparative example 2
Substantially with embodiment 1, difference is Extraction solvent methanol replacing with ether assay method.
According to the method described above, it is parallel to take 3 parts of samples to be tested, as a result such as following table：
As a result：As seen from the above table, when Extraction solvent being changed to ether, extraction effect is poor, the vitamin E in sample to be tested
It cannot extract completely, cause testing result relatively low, and the precision detected is low, reproducibility is poor.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Embodiment described above only expresses the several embodiments of the present invention, and description is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that those of ordinary skill in the art are come
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Range.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of assay method of food vitamins E contents, which is characterized in that include the following steps：
(1) preparation of test solution：
Sample to be tested is taken to be dissolved in methanol, after vortex mixed, ultrasonic extraction and centrifugation, takes the supernatant liquor mistake after centrifugation
Filter, obtains test solution；
(2) Ultra Performance Liquid Chromatography instrument measures：
The test solution is taken, the content of vitamin E in the test solution, institute are measured using Ultra Performance Liquid Chromatography instrument
The condition for stating Ultra Performance Liquid Chromatography instrument measure includes：
Chromatographic column：C18 (150mm × 4.6mm, 5 μm)；
Mobile phase：Methanol；Flow velocity：0.6~1.2mL/min.
2. the assay method of food vitamins E contents according to claim 1, which is characterized in that the ultra high efficiency liquid
The condition that chromatography measures further includes：
Column temperature：25-35；
Sample size：9-11.
3. the assay method of food vitamins E contents according to claim 2, which is characterized in that the ultra high efficiency liquid
The condition that chromatography measures includes：
Chromatographic column：C18 (150mm × 4.6mm, 5 μm)；
Mobile phase：Methanol；Flow velocity：1.0mL/min；
Column temperature：30℃；
Sample size：10μL.
4. the assay method of food vitamins E contents according to claim 1, which is characterized in that the sample to be tested
Proportioning with the methanol is 0.15-0.25g：10mL.
5. the assay method of food vitamins E contents according to claim 4, which is characterized in that the sample to be tested
Proportioning with the methanol is 0.2g：10mL.
6. according to the assay method of claim 1-5 any one of them food vitamins E contents, which is characterized in that described
The time of ultrasonic extraction is 14-30min.
7. the assay method of food vitamins E contents according to claim 6, which is characterized in that the ultrasonic extraction
Time be 14-16min.
8. according to the assay method of claim 1-5 any one of them food vitamins E contents, which is characterized in that described
The time of vortex mixed is 3-5min.
9. according to the assay method of claim 1-5 any one of them food vitamins E contents, which is characterized in that described
The time of centrifugation is 1-2min, and the rotating speed of the centrifugation is 3.0 × 103-4.0×103rpm。
10. according to the assay method of claim 1-5 any one of them food vitamins E contents, which is characterized in that described
Filtering uses the filter membrane that aperture is 0.45 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711349912.XA CN108195956A (en) | 2017-12-15 | 2017-12-15 | The assay method of food vitamins E contents |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711349912.XA CN108195956A (en) | 2017-12-15 | 2017-12-15 | The assay method of food vitamins E contents |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108195956A true CN108195956A (en) | 2018-06-22 |
Family
ID=62574492
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711349912.XA Pending CN108195956A (en) | 2017-12-15 | 2017-12-15 | The assay method of food vitamins E contents |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108195956A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110441430A (en) * | 2019-08-22 | 2019-11-12 | 山东中谷饲料有限公司 | A method of vitamin E in detection animal tissue and egg |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104614479A (en) * | 2015-01-30 | 2015-05-13 | 北京市营养源研究所 | Detection method of vitamin in food |
| CN105116073A (en) * | 2015-09-08 | 2015-12-02 | 崇州市白头甘泉工业油脂厂 | Determination method for detecting content of natural vitamin E in palm oil |
| CN105467031A (en) * | 2015-11-25 | 2016-04-06 | 江苏艾兰得营养品有限公司 | Chromatographic method for measuring content of vitamin E by HPLC |
| CN106855545A (en) * | 2016-12-26 | 2017-06-16 | 新希望六和股份有限公司 | Liposoluble vitamin simultaneously in detection feed and the method for water soluble vitamin |
-
2017
- 2017-12-15 CN CN201711349912.XA patent/CN108195956A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104614479A (en) * | 2015-01-30 | 2015-05-13 | 北京市营养源研究所 | Detection method of vitamin in food |
| CN105116073A (en) * | 2015-09-08 | 2015-12-02 | 崇州市白头甘泉工业油脂厂 | Determination method for detecting content of natural vitamin E in palm oil |
| CN105467031A (en) * | 2015-11-25 | 2016-04-06 | 江苏艾兰得营养品有限公司 | Chromatographic method for measuring content of vitamin E by HPLC |
| CN106855545A (en) * | 2016-12-26 | 2017-06-16 | 新希望六和股份有限公司 | Liposoluble vitamin simultaneously in detection feed and the method for water soluble vitamin |
Non-Patent Citations (4)
| Title |
|---|
| 周围等: "超高效合相色谱法同时测定复合维生素片中11 种脂溶性维生素及其衍生物", 《分析化学》 * |
| 戚燕等: "HPLC 法测定非诺贝特软胶囊中维生素E的含量", 《中国药品标准》 * |
| 田守生等: "HPLC法测定阿胶养颜软胶囊中维生素E的含量", 《齐鲁药事》 * |
| 董敬翠: "HPLC法测定六维磷脂软胶囊中维生素E的含量", 《黑龙江中医药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110441430A (en) * | 2019-08-22 | 2019-11-12 | 山东中谷饲料有限公司 | A method of vitamin E in detection animal tissue and egg |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106770769B (en) | A kind of method of a variety of liposoluble vitamins in detection feed | |
| CN102662009B (en) | Method for quickly determining content of additive of plurality of types in food | |
| CN102998389B (en) | Gas chromatography detection method of corrosion removers in food | |
| CN105891384B (en) | A kind of detection method of liposoluble vitamin content | |
| CN105777692B (en) | Isoflavones component is extracted and assay method | |
| CN105998109A (en) | Mango seed polyphenol extract and preparation method thereof | |
| CN105301134A (en) | Method for estimating in-vitro detoxification effect of mycotoxin detoxification agent by using liquid chromatography | |
| CN103033578B (en) | Method for quickly and preliminarily screening excellent tea tree resource based on contained contents | |
| Huang et al. | Determination of Vitamins D2 and D3 in Edible Fungus by Reversed‐Phase Two‐Dimensional Liquid Chromatography | |
| CN105866310A (en) | Method for measuring contents of organic acids in blueberries | |
| CN102128827B (en) | Method for detecting trace lipid-soluble tea polyphenol in vegetable oil | |
| CN108195956A (en) | The assay method of food vitamins E contents | |
| CN103760289A (en) | Extraction and high efficiency liquid-phase measurement method for anthocyanin in blood-flesh peach fruits | |
| CN105527354A (en) | Quantitative determination method of lutein content in high-fat sample | |
| CN108008060A (en) | The assay method and reagent of hydroxyproline in a kind of feed | |
| CN103520243B (en) | A kind of cyclic adenosine monophosphate snow chrysanthemum oil resin composite soft capsule and preparation method thereof | |
| CN101278727B (en) | Preparation of blackberry pigment | |
| CN104483411B (en) | A kind of capsule of weeping forsythia and the detection method containing capsule of weeping forsythia product | |
| CN103091408B (en) | Method for determining content of hesperidin in Citrus Bioflavonoids | |
| CN105693679A (en) | Method for preparing high-purity GC (gallocatechin) | |
| CN109725093A (en) | The detection method of synthetic coloring matter in a kind of food | |
| CN110749684A (en) | Rapid detection method for organic acid in enzyme | |
| CN106198798A (en) | A kind of detection method of cholesterol level | |
| CN110151811A (en) | A kind of composition and application thereof containing flue berry extract | |
| CN105136939B (en) | A kind of method that use HPLC methods determine citric acid content in mulberries medicinal material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180622 |
|
| RJ01 | Rejection of invention patent application after publication |