CN105998109A - Mango seed polyphenol extract and preparation method thereof - Google Patents
Mango seed polyphenol extract and preparation method thereof Download PDFInfo
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- CN105998109A CN105998109A CN201610471546.4A CN201610471546A CN105998109A CN 105998109 A CN105998109 A CN 105998109A CN 201610471546 A CN201610471546 A CN 201610471546A CN 105998109 A CN105998109 A CN 105998109A
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- Prior art keywords
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- mangiferae indicae
- semen mangiferae
- polyphenol
- indicae
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- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 101
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 100
- 238000000605 extraction Methods 0.000 title claims abstract description 46
- 239000000284 extract Substances 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 235000014826 Mangifera indica Nutrition 0.000 title abstract description 9
- 235000004936 Bromus mango Nutrition 0.000 title abstract description 8
- 235000009184 Spondias indica Nutrition 0.000 title abstract description 8
- 240000007228 Mangifera indica Species 0.000 title description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 133
- 239000011347 resin Substances 0.000 claims abstract description 49
- 229920005989 resin Polymers 0.000 claims abstract description 49
- 238000000034 method Methods 0.000 claims abstract description 34
- 239000003814 drug Substances 0.000 claims abstract description 10
- 235000013305 food Nutrition 0.000 claims abstract description 4
- 210000000582 semen Anatomy 0.000 claims description 97
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- 239000007788 liquid Substances 0.000 claims description 28
- 239000000843 powder Substances 0.000 claims description 22
- 239000003480 eluent Substances 0.000 claims description 17
- 238000010828 elution Methods 0.000 claims description 16
- 238000004821 distillation Methods 0.000 claims description 14
- 238000001035 drying Methods 0.000 claims description 9
- 239000012535 impurity Substances 0.000 claims description 9
- 239000000047 product Substances 0.000 claims description 9
- 239000003463 adsorbent Substances 0.000 claims description 7
- 239000012264 purified product Substances 0.000 claims description 7
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 6
- 230000036541 health Effects 0.000 claims description 5
- 238000004090 dissolution Methods 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 4
- 238000011068 loading method Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 238000010992 reflux Methods 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- 238000007598 dipping method Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 238000002386 leaching Methods 0.000 claims description 2
- NDTDVKKGYBULHF-UHFFFAOYSA-N 2-(1-hydroxy-3-phenylnaphthalen-2-yl)-3-phenylnaphthalen-1-ol Chemical compound C=1C2=CC=CC=C2C(O)=C(C=2C(=CC3=CC=CC=C3C=2O)C=2C=CC=CC=2)C=1C1=CC=CC=C1 NDTDVKKGYBULHF-UHFFFAOYSA-N 0.000 claims 1
- 241001093152 Mangifera Species 0.000 abstract description 24
- 230000000694 effects Effects 0.000 abstract description 9
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 8
- 238000000746 purification Methods 0.000 abstract description 8
- 230000003078 antioxidant effect Effects 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 5
- 239000003963 antioxidant agent Substances 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 238000012827 research and development Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 63
- 229960004756 ethanol Drugs 0.000 description 45
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 26
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 18
- 239000000523 sample Substances 0.000 description 17
- 229960003742 phenol Drugs 0.000 description 13
- 238000001179 sorption measurement Methods 0.000 description 13
- 238000002835 absorbance Methods 0.000 description 11
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- 238000004080 punching Methods 0.000 description 3
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- NGSWKAQJJWESNS-UHFFFAOYSA-N 4-coumaric acid Chemical compound OC(=O)C=CC1=CC=C(O)C=C1 NGSWKAQJJWESNS-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 2
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- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 2
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- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
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- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 1
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- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 1
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- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 description 1
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- 238000001514 detection method Methods 0.000 description 1
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 1
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- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
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- 244000144972 livestock Species 0.000 description 1
- KKSDGJDHHZEWEP-UHFFFAOYSA-N m-hydroxycinnamic acid Natural products OC(=O)C=CC1=CC=CC(O)=C1 KKSDGJDHHZEWEP-UHFFFAOYSA-N 0.000 description 1
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- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 1
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- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/22—Anacardiaceae (Sumac family), e.g. smoketree, sumac or poison oak
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention discloses a mango seed polyphenol extract and a preparation method thereof and belongs to the field of food technologies and biotechnologies. The mango seed polyphenol extract takes mango seeds as a raw material and is prepared with an alcohol extraction and macroporous resin purification method. The prepared mango seed polyphenol extract has the significant anti-bacterial and antioxidant activity effects and can be used as an antioxidant health-care product or medicine for further research and development.
Description
Technical field
The present invention relates to a kind of Semen Mangiferae indicae polyphenol extract and preparation method thereof, belong to food technology and biotechnology neck
Territory.
Background technology
Fructus Mangifera Indicae (Classification system: Mangifera indica Linn), also known as mango, vexed fruit, honey shop sign in the form of a streamer etc., belongs to Toxicodendron verniciflnum (Stokes) F. A. Barkley (Rhus verniciflua Stokes)
Section plant, primary growth is in the coastal Intertidal zone of Perenniporia martius.The plantation distribution of Fructus Mangifera Indicae is very wide, has more than 70 country in the world
Producing Fructus Mangifera Indicae, Guangxi, China main province, Guangdong, Taiwan, Hainan all have Fructus Mangifera Indicae to plant, and Mango Resources is abundant, various in style.
Within 2014, China's Fructus Mangifera Indicae cultivated area is 1,170,000 hectares, and total output reaches 880,000 tons, is large tradition torrid zone agricultural product of China,
Foreign exchange earning has a high potential, and has consequence in China's Foreign Trade.
Fructus Mangifera Indicae is one of famous tropical fruit (tree), and because of its sarcocarp fine and smooth (sliding tender, the succulence of meat), (taste is fragrant for unique flavor
Sweet), nutritious (rich in sugar, protein, crude fibre, vitamin, mineral and fat etc.), collect the torrid zone fruit essences in one
Body, is described as " king of tropical fruit (tree) ".Fructus Mangifera Indicae has high medical value, and its peel can be used as medicine, and for diuresis, dredges lower dose；Core
Renhe Folium mangiferae also can be used as medicine, stagnant, the blood pressure lowering that disappears of detoxifying.According to traditional Chinese medical science analysis, the flat sweet in the mouth of belonging to property of Fructus Mangifera Indicae, the fruit promoted the production of body fluid of quenching one's thirst
Product, have stomach reinforcing, preventing or arresting vomiting, anti-corona effect.The chimonin contained in Fructus Mangifera Indicae have obvious anti-peroxidation, protection brain neuron and
Effect of expelling phlegm for arresting cough.
Guangxi produces one of Fructus Mangifera Indicae main province few in number as China, has Mango Resources especially and enriches, kind
The advantage such as various, but main in the market or based on place of production marketing fresh, being processed into fruit juice, preserved fruit and sarcocarp on a small quantity is
Outside auxiliary, Fructus Mangifera Indicae process deeply industry urgently needs to develop further.Garbage in Fructus Mangifera Indicae production and processing, was often dropped in the past
Or raise livestock.In recent years, Fructus Mangifera Indicae garbage is gradually studied by people, including Folium mangiferae, mangrove bark, Semen Mangiferae indicae, awns
Fruit tree skins etc., the Semen Mangiferae indicae especially accounting for Fructus Mangifera Indicae gross weight 20-60% obtains major part researcher and the concern of enterpriser.According to report
Road, in Fructus Mangifera Indicae, chemical composition is of a great variety, mainly based on polyphenol, including chimonin, Quercetin, kaempferol, para hydroxybenzene first
Acid, m-Coumaric Acid, P-coumaric acid and ferulic acid etc., and major part active component is positioned at the Fructus Mangifera Indicae accounting for Fructus Mangifera Indicae gross weight 20-60%
In core.
Semen Mangiferae indicae rich in have aminoacid, polypeptide, protein, phenols, flavone, polysaccharide, organic acid, saponin, tannin, flavone,
The various active compositions such as anthraquinone, alkaloid, coumarin and lactone, triterpene and steroidal, alkaloid, volatile oil and oils and fats.
In recent years, China around Semen Mangiferae indicae polyphenols, flavonoid, pyrrones, terpenoid isoreactivity composition and antibacterial,
The correlational study that anti-oxidation function is carried out is the most more, but there is also many problems, mainly has extraction separating effect inconspicuous,
Identifying that the active component obtained is less, the pharmacological action such as active component is antibacterial, antioxidation is the most indefinite.
Existing Semen Mangiferae indicae polyphenol extracting method, there is also the Determination of Polyphenols of extraction low, and organic solvent residual is more
Problem.
Summary of the invention
It is an object of the invention to make full use of the natural resources, especially Guangxi characteristic fruit and vegerable resource, it is provided that a kind of Semen Mangiferae indicae
Polyphenol extract and preparation method thereof, this polyphenol extract has antibacterial antioxygenic property, can be used for health product or medicine system
Standby.In order to solve to have at present, the Determination of Polyphenols of Semen Mangiferae indicae polyphenol extracting method existence is low, organic solvent residual is more simultaneously
Problem, the inventive method is to prepare Semen Mangiferae indicae polyphenol extract through alcohol extraction and purification by macroporous resin method.
The preparation method of the Semen Mangiferae indicae polyphenol extract of the present invention, comprises the following steps:
(1) by Semen Mangiferae indicae drying and crushing；
(2) with the ethanol solution of determining alcohol 20-90% (v/v) or methanol solution as solvent to step (1) obtains
Semen Mangiferae indicae dried powder extracts, then by the extracting solution concentrating under reduced pressure obtained or vacuum lyophilization to extractum；
(3) by the extractum water obtained in step (2), determining alcohol less than the ethanol solution of 50% (v/v) or determining alcohol
Methanol solution less than 50% (v/v) dissolves, and removes insoluble matter, obtains load solution；
(4) negated polarity or low pole macroporous adsorbent resin, the load solution loading that step (3) is obtained, first wash with water
Decontamination, then carries out eluting with eluant (ethanol solution or methanol solution), collects eluent；Described macroporous adsorbent resin and
Semen Mangiferae indicae dried powder weight ratio is 1:0.5-1:20；
(5) eluent concentrating under reduced pressure step (4) collected or vacuum lyophilization, obtain Semen Mangiferae indicae polyphenol extract.
In one embodiment of the invention, in step (2), extracting method is that every kilogram of Semen Mangiferae indicae powder adds 2-50L
Determining alcohol is ethanol solution or the methanol solution of 30%-80%, and hot reflux or dipping or leaching are filtered and extracted 2-5 time, circumfluence distillation
Time extract 1-3h every time, impregnate or soak filter extraction time extract 10-15 days every time, united extraction liquid.
In one embodiment of the invention, the extraction of described step (2), is with 10-95% (v/ by Semen Mangiferae indicae powder
V, lower with) ethanol extraction, preferred alcohol concentration is 20%-80%, most preferably 40%-60%.
In one embodiment of the invention, the extraction of described step (2), is with ethanol extraction by Semen Mangiferae indicae powder
Number 1-4 time, most preferably 2-3 time.
In one embodiment of the invention, the extraction of described step (2), solid-liquid ratio (Semen Mangiferae indicae powder quality and second
The ratio of alcoholic solution, m/v) it is 1:1-1:40, preferably 1:5-1:30, more preferably 1:15-1:25.
In one embodiment of the invention, the extraction of described step (2), each extraction time is 0.5-4h, preferably
Extraction time is 1-2h.
In one embodiment of the invention, the extraction of described step (2), is to carry out at 40-60 DEG C.
In one embodiment of the invention, described step (2) concrete technology condition is: concentration of alcohol 70%, feed liquid
Ratio 1:25, Extracting temperature 60 DEG C, extraction time 120min.
In one embodiment of the invention, the water used by dissolving of extractum in described step (3), ethanol solution or
The weight of methanol solution is 3-50 times of extractum weight.
In one embodiment of the invention, the extracting method of step (3) is as follows: the extractum that will obtain in step (2)
Water or 10% ethanol solution by 10 times of extractum weight dissolve, and obtain load solution after removing insoluble matter.
In one embodiment of the invention, the removing insoluble matter of step (3), is to use the method filtering or being centrifuged.
In one embodiment of the invention, the type of the macroporous adsorbent resin of step (4) selects HPD-722, HPD-
826, LX-17, LX-26, LX-28, SP70, SP700, AB-8, DM21 or D101, preferably macroporous resin AB-8, DM21,
D101, more preferably AB-8.
In one embodiment of the invention, step (4) first washes decontamination with water, refer to enter with the water of 2-5BV
Row eluting is to remove impurity.
In one embodiment of the invention, the elution flow rate of described step (4) is eluent flow rate 1BV/h-10BV/
H, preferably 2BV/h-5Bv/h.
In one embodiment of the invention, eluant ethanol solution or methanol solution dense in described step (4)
Degree is 5%-95%, preferably 20%-80%；The elution volume of eluant is 1-10 times of column volume, preferably 3-8 cylinder
Long-pending.
In one embodiment of the invention, described step (4), be use determining alcohol be the ethanol solution of 40-70%
Carry out eluting.
In one embodiment of the invention, the purification process of step (4) is as follows: takes and with Semen Mangiferae indicae powder weight ratio is
The low pole macroporous resin of 1:5, the load solution loading that will obtain in step (3), use 2-5BV washing decontamination, after adopt
Carry out eluting with 50% ethanol solution of 3-10BV, collect the eluent that the ethanol solution of 50% affords.
In one embodiment of the invention, in described step (4), the applied sample amount (Fructus Mangifera Indicae of Semen Mangiferae indicae polyphenol extracting solution
Core powder quality: resin quality) it is 0.5:1-20:1, preferably 1:1-10:1.
In one embodiment of the invention, described method is: with ethanol solution heating and refluxing extraction Semen Mangiferae indicae polyphenol,
Reclaim ethanol, concentrate, Semen Mangiferae indicae polyphenol extracting solution is adsorbed onto on macroporous resin, eluting, collection eluent, concentrate, be dried, obtain
Obtain the Semen Mangiferae indicae polyphenol of purification.
In one embodiment of the invention, described method is specifically: (1) is by Semen Mangiferae indicae drying and crushing；(2) awns is weighed
It is the ethanol solution of 50% that pit powder 1kg adds 20L concentration, and circumfluence distillation three times, each 1h of circumfluence distillation, merging carries
Take liquid, extracting solution concentrating under reduced pressure juice extractum；(3) the extractum water dissolution of extractum weight 10 times, filters away insoluble matter, obtain on
Sample solution；(4) by load solution upper prop, controlling effluent flow velocity is 0.5BV/h, and applied sample amount and Resin A B-8 volume ratio are 2:1,
Resin bed is fully entered to extracting solution；The water using 2-5BV carries out eluting, to remove impurity, then with the 40 of 3-10BV～
60% ethanol solution rinses resin bed, controls effluent flow velocity 2BV/h, collects the eluent of ethanol solution；(5) by washing of collecting
De-liquid is evaporated to certain volume, is spray-dried, and obtains purified product.
The present invention is also claimed the Semen Mangiferae indicae polyphenol extract obtained according to described preparation method or purification process and exists
Prepare the application on food, health product or medicine.
Described health product or medicine, be health product or the medicine with antibacterial antioxidant activity.
Beneficial effects of the present invention:
1, the present invention is by improving Semen Mangiferae indicae polyphenol extraction step, is effectively increased the extraction effect of polyphenol substance
Rate, in the extracting solution obtained, polyphenol content (up to 18-20.9mg/g be i.e. can extract in 1kg Semen Mangiferae indicae dried powder and obtain 18-
20.9g polyphenol).And, the inventive method is with water or ethanol as solvent, the most cheap but also nontoxic.
2, the present invention uses the method for purification by macroporous resin to be purified extracting solution further, the operation letter of this purification process
Just, fast, the macroporous resin of selection has the advantages that adsorbance is big, desorption efficiency is big, effectively eliminates the impurity of extracting solution, prevents
The problem of organic solvent residual.
3, the Semen Mangiferae indicae polyphenol extract obtained according to the inventive method, yield, up to 3.6-20.9mg/g, obtains many
Phenol extraction thing has obvious scavenging action to hydroxyl and Superoxide radicalanion.
Accompanying drawing explanation
Fig. 1 is gallic acid canonical plotting；
Fig. 2 is the scavenging action to hydroxyl of the Semen Mangiferae indicae polyphenol extract；
Fig. 3 is the scavenging action to Superoxide radicalanion of the Semen Mangiferae indicae polyphenol extract；
Fig. 4 is the scavenging action to DPPH of the Semen Mangiferae indicae polyphenol extract.
Detailed description of the invention
Being described further the technology contents of the present invention below in conjunction with embodiment, following embodiment is illustrative, no
It is determinate, it is impossible to limit protection scope of the present invention with following embodiment.
The equipment together, chemical reagent and the detection method that use in each embodiment are as follows:
Instrument and equipment: FW100 high speed Universalpulverizer, Tianjin Stettlen Instrument Ltd.；UPC-II-20T is excellent
General series Superpure water machine, Sichuan UP Hyperpure Technology Co., Ltd.；SQP electronic balance, Sai Duolisi scientific instrument (Beijing) have
Limit company；ZWYR-D2403 shaken cultivation case, Shanghai Zhi Cheng analytical tool Manufacturing Co., Ltd；SHZ-D III gives China's board recirculated water
Vacuum pump, Yuhua Instrument Co., Ltd., Gongyi City；HHS-6S electronic thermostatic stainless steel water bath, Yichang, Shanghai instrument yarn sieves
Factory；UV1901PC ultraviolet-uisible spectrophotometer, Shanghai Ao Xi scientific instrument company limited；
Chemical reagent: natrium carbonicum calcinatum, methanol, ethanol, acetone, ethyl acetate, forint phenol reagent, gallic acid standard
Product, all kinds macroporous resin etc. are provided by Liuzhou Su Li Co., Ltd.
Measure:
The foundation of gallic acid standard curve: accurately weigh 50mg gallic acid, is settled to 50ml with 70% ethanol solution,
Obtaining concentration is 1.0mg/ml gallic acid standard solution.Take 9 50ml volumetric flasks, pipette 0 respectively, 1,2,3,4,5,7.5,
10,12.5ml above-mentioned gallic acid standard solution, be made into 0 after being diluted, 0.02,0.04,0.06,0.08,0.10,0.15,
0.20, the titer of 0.25mg/ml.Absorbance is measured with folin's methods.With gallic acid concentration as abscissa, at 765nm
Absorbance is vertical coordinate, draws standard curve, and carries out regression analysis.Forint phenol method measures the standard curve (Fig. 1) of total phenols.
Result shows, gallic acid is good linear relationship with its light absorption value in concentration 0-0.25mg/ml, line in this range
Property regression equation is: y=4.2974x+0.056, R2=0.9994.
The mensuration of total phenol content: accurately pipette liquid 2.0ml to be measured in 50ml volumetric flask, adds Folin reagent 1.0ml, fills
Stand 2min after dividing mixing, add the sodium carbonate liquor of 10ml 7%, use distilled water constant volume after being sufficiently mixed, put 30 DEG C of thermostatted waters
Bath is reacted 1h, constantly vibrates.Take out, at wavelength 765nm, measure absorbance (with distilled water as blank).According to mark
Directrix curve and regression equation thereof obtain the polyphenol content in liquid to be measured, then calculate 1g Semen Mangiferae indicae and are dried the powder-like after crushing
In product polyphenols content (in terms of gallic acid, mg/g, i.e. polyphenol content mg/ Semen Mangiferae indicae powder g).
The mensuration of antioxidant activity:
Remove hydroxyl radical free radical ability to measure: take different quality concentration respectively treats that sample measuring liquid, in 5 test tubes, respectively adds
Ferrous sulfate, hydrogen peroxide, stand, and adds salicylic acid after 10min, measures absorbance A after standing at 510nm1, replace with distilled water
Absorbance A is measured according to the method described above for salicylic acid2, replace liquid to be measured to measure absorbance A as stated above with distilled water0,
VCCompare, seek the polyphenol substance clearance rate to hydroxy radical according to the following formula: hydroxy radical (%)=[A0-(A1-A2)]/
(A0) * 100 (%).
Remove ultra-oxygen anion free radical ability to measure: take 4.5ml Tris-HCl buffer solution, pre-in 20 DEG C of water-baths
Hot 20min, is separately added into the sample liquid of 1ml variable concentrations and the pyrogallol solution of 0.4ml 25mmol/L, after mixing, in 25
Reacting 5min in DEG C water-bath, the HCl solution 1ml being subsequently adding 8mol/L terminates reaction, then mensuration absorbance 325nm at
A1, blank with distilled water replacement sample liquid, measure absorbance A0.The sample clearance rate to ultra-oxygen anion free radical: clearance rate
(%)=(1-A1/A0) * 100 (%).
DPPH Scavenging activity measure: take respectively 2.0mL different quality concentration (1.0,5.0,10.0,15.0, Semen Mangiferae indicae activity
Composition sample liquid is in 5 test tubes, and each 2mL DPPH (0.04mg/ml) that adds, standing 20min detect absorbance A at 517nm1；With
Dehydrated alcohol replaces DPPH, measures absorbance A according to the method described above2；Do blank group and measure absorbance A as stated above0, with VC
Compare, the sample clearance rate to DPPH free radical: DPPH clearance rate (%)=[1-(A1-A2)/A0] * 100 (%).
Embodiment 1: the preparation method of the Semen Mangiferae indicae polyphenol extract of the present invention
(1) the many phenol extractions of Semen Mangiferae indicae: Semen Mangiferae indicae drying and crushing, weigh Semen Mangiferae indicae powder 1kg adding 20L concentration is 50%
Ethanol, circumfluence distillation three times, each 1h of circumfluence distillation, united extraction liquid, extracting solution concentrating under reduced pressure juice extractum, extractum soaks
The water dissolution that cream weight is 10 times, filters away insoluble matter, obtains load solution, and Determination of Polyphenols is 18-20.9g/kg Semen Mangiferae indicae
Powder.
(2) upper prop: by Semen Mangiferae indicae polyphenol extracting solution upper prop, controlling effluent flow velocity is 0.5BV/h, applied sample amount and resin
AB-8 volume ratio is 2:1, fully enters resin bed to extracting solution.
(3) eluting: use the water of 2-5BV to carry out eluting, to remove impurity, after be respectively adopted the 5%-80% second of 3-10BV
Alcohol rinses resin bed, controls effluent flow velocity 2BV/h, collects eluent, is evaporated to certain volume respectively, carries out spray dried
Dry, obtain the purified product of different component, wherein all component Determination of Polyphenols is about 20.9g/kg, in purified product, washing
In de-liquid, polyphenol content is about 2.2g/kg, and in 5% ethanol elution, polyphenol content is about 3.8g/kg, in 10% ethanol elution
Polyphenol content is about 4.8g/kg, and in 20% ethanol elution, polyphenol content is about 8.04g/kg, polyphenol in 30% ethanol elution
Content is about 8.5g/kg, and in 40% ethanol elution, polyphenol content is about 13.3g/kg, polyphenol content in 50% ethanol elution
Being about 15.2g/kg, in 60% ethanol elution polyphenol, content is about 13.7g/kg, and in 70% ethanol elution, polyphenol content is about
For 11.1g/kg, in 80% ethanol elution, polyphenol content is about 9.9g/kg.
Embodiment 2: select different condition to extract
Using the method that the present invention provides, selection carries out Semen Mangiferae indicae polyphenol without condition, and (sample is Semen Mangiferae indicae drying and crushing
1g) extract experiment, on the basis of single factor experiment, choose concentration of alcohol, solid-liquid ratio, extraction time and Extracting temperature four main
Factor is L9(34) orthogonal test (table 1), orthogonal interpretation of result such as table 2.
Table 1 Semen Mangiferae indicae polyphenol extraction technology orthogonal test gauge outfit designs
Table 2 Semen Mangiferae indicae polyphenol extraction technology L9(34) Orthogonal experiment results
The checking test of table 3 Semen Mangiferae indicae many phenol extractions
As shown in Table 2, each factor phenol extraction many on Semen Mangiferae indicae affects size order and is: B > A > D > C, i.e. solid-liquid ratio > ethanol
Concentration > extraction time > Extracting temperature, wherein the impact of solid-liquid ratio phenol extraction many on Semen Mangiferae indicae is the most notable, next to that concentration of alcohol and
Extraction time, affecting significance the same, the impact of Extracting temperature phenol extraction many on Semen Mangiferae indicae is the most notable.Can by orthogonal experiments
To show that optimal test combinations is as A3B3C3D3, i.e. concentration of alcohol 70%, solid-liquid ratio 1:25, Extracting temperature 60 DEG C, extraction time
120min.Being not mutually orthogonal in the combination of table, so needing to do checking test (table 3), checking result of the test understanding polyphenol
Extracting content is 5.36mg/g, so the optimum process condition of the many phenol extractions of Semen Mangiferae indicae is concentration of alcohol 70%, and solid-liquid ratio 1:25,
Extracting temperature 60 DEG C, extraction time 120min.
Embodiment 3: purification by macroporous resin technique
Choose the factors such as different resins kind, sample liquid concentration, the rate of adsorption, applied sample amount, ethanol elution concentration, elution rate
Carry out single factor experiment respectively, determine the optimum of each factor.
Prepared by sample solution: weigh Semen Mangiferae indicae powder 20g, according to concentration of alcohol 70%, solid-liquid ratio 1:25, Extracting temperature 60
DEG C, to extract 1h every time, extract 3 times, after extraction, filtrate merges, and is evaporated to do, is dried under vacuum to constant weight, and precision weighs dry cream
In right amount, it is dissolved in 10ml volumetric flask with methanol or ethanol solution, adds methanol or ethanol solution is diluted to scale, shake up standby.Move
Take 1ml filtrate to 10ml volumetric flask, add methanol or ethanol solution is diluted to scale, shake up.Measure polyphenol according to the method described above
Content.
Static adsorption: take 10 kinds through each 10g of macroporous adsorbent resin that pretreatment is good, each add the many phenol solutions of Semen Mangiferae indicae
10ml, every 10min shake once, respectively take the solution after resin absorption and measure polyphenol content, calculate each resin to awns after 2h
The adsorption rate of pit polyphenol.
Static desorbing:
Being drained by the resin filter of static adsorption, add 30ml 50% ethanol desorbing, every 10min shakes once, after 2h respectively
Respectively take the solution after resin absorption and measure polyphenol content, calculate each resin desorption efficiency to Semen Mangiferae indicae polyphenol.It is shown in Table 4.From table
It can be seen that the adsorption rate of 10 kinds of resins is the biggest (more than 90%), desorption efficiency is both greater than 40%, wherein AB-8, DM21, D101
Desorption efficiency be both greater than 60%, choose AB-8, DM21, D101 do dynamic adsorption experiment.
Table 4 macroporous resin adsorption and desorption rate to Semen Mangiferae indicae polyphenol
Dynamic adsorption:
Take preferably 3 kinds Resin A B-8 of the static adsorption handled well, each 15mL of DM21, D101 in post, add Semen Mangiferae indicae polyphenol
Extracting solution, in capital, carries out dynamic adsorption with the flow velocity of 0.5BV/h, flows out according to resin volume and collects effluent, measures polyphenol
Content, calculates each resin adsorption rate to Semen Mangiferae indicae polyphenol.The results are shown in Table 5, show when AB-8 and D101 reaches saturated absorption, institute
The total polyphenols amount of absorption is big compared with DM21, chooses it and does desorption experiment.
Table 5 macroporous resin dynamic adsorption result to Semen Mangiferae indicae polyphenol
Dynamic desorption: take each 20ml of AB-8 and D101 resin handled well in post, add Semen Mangiferae indicae polyphenol extracting solution respectively
(10.5mg/g), in capital, after adsorbing with the flow velocity of 0.5BV/h, carry out eluting with 60% ethanol with the flow velocity of 2BV/h, press
Flow out according to resin volume and collect eluent, measure polyphenol content, calculate each resin adsorption rate to Semen Mangiferae indicae polyphenol.The results are shown in Table
6, it can be seen that AB-8 is better than D101 desorption effect, chooses it as optimum resin.
Table 6 macroporous resin dynamic desorption result to Semen Mangiferae indicae polyphenol
Concentration of alcohol: take 10 parts of AB-8 resin 20ml handled well in post, adds Semen Mangiferae indicae polyphenol extracting solution in capital, with
After the flow velocity of 0.5BV/h adsorbs, more respectively with the water of 5BV, 5%, 10%, 20%, 30%, 40%, 50%, 60%,
70%, 80% ethanol carries out independent eluting with 2BV/h flow velocity, flows out according to resin volume and collects eluent, measures polyphenol content,
The results are shown in Table 7, it can be seen that when ethanol elution concentration is 40%-70%, eluting total polyphenols yield is the highest.
Table 7 ethanol elution concentration is investigated
Embodiment 4: the preparation method of Semen Mangiferae indicae polyphenol extract
By Semen Mangiferae indicae drying and crushing, weigh Semen Mangiferae indicae powder 1kg adding 20L concentration is the ethanol of 50%, circumfluence distillation
Three times, each 1h of circumfluence distillation, united extraction liquid, extracting solution concentrating under reduced pressure juice extractum, the extractum water of extractum weight 10 times
Dissolving, filter away insoluble matter, obtain load solution, Determination of Polyphenols is 18-20.9g/kg.
By Semen Mangiferae indicae polyphenol extracting solution upper prop, controlling effluent flow velocity is 0.5BV/h, applied sample amount and Resin A B-8 volume ratio
For 2:1, fully enter resin bed to extracting solution.The water using 2-5BV removes impurity, then uses the 50% ethanol punching of 3-10BV
Wash resin bed, control effluent flow velocity 2BV/h, collect eluent, be evaporated to certain volume respectively, be spray-dried,
Obtaining purified product, polyphenol content is 14-15.2g/kg.
Embodiment 5: the preparation method of Semen Mangiferae indicae polyphenol extract
By Semen Mangiferae indicae drying and crushing, weigh Semen Mangiferae indicae powder 1kg adding 20L concentration is the ethanol of 50%, circumfluence distillation
Three times, each 1h of circumfluence distillation, united extraction liquid, extracting solution concentrating under reduced pressure juice extractum, the extractum water of extractum weight 10 times
Dissolving, filter away insoluble matter, obtain load solution, Determination of Polyphenols is 18-20.9g/kg.
By Semen Mangiferae indicae polyphenol extracting solution upper prop, controlling effluent flow velocity is 0.5BV/h, applied sample amount and Resin A B-8 volume ratio
For 2:1, fully enter resin bed to extracting solution.The water using 2-5BV removes impurity, then uses the 40% ethanol punching of 3-10BV
Wash resin bed, control effluent flow velocity 2BV/h, collect eluent, be evaporated to certain volume respectively, be spray-dried,
Obtaining purified product, polyphenol content is 12-13.3g/kg.
Embodiment 6: the preparation method of Semen Mangiferae indicae polyphenol extract
By Semen Mangiferae indicae drying and crushing, weigh Semen Mangiferae indicae powder 1kg adding 20L concentration is the ethanol of 50%, circumfluence distillation
Three times, each 1h of circumfluence distillation, united extraction liquid, extracting solution concentrating under reduced pressure juice extractum, the extractum water of extractum weight 10 times
Dissolving, filter away insoluble matter, obtain load solution, Determination of Polyphenols is 18-20.9g/kg.
By Semen Mangiferae indicae polyphenol extracting solution upper prop, controlling effluent flow velocity is 0.5BV/h, applied sample amount and Resin A B-8 volume ratio
For 2:1, fully enter resin bed to extracting solution.The water using 2-5BV removes impurity, then uses the 70% ethanol punching of 3-10BV
Wash resin bed, control effluent flow velocity 2BV/h, collect eluent, be evaporated to certain volume respectively, be spray-dried,
Obtaining purified product, polyphenol content is 10-11.1g/kg.
Embodiment 7: the antibacterial antioxygenic property of the Semen Mangiferae indicae polyphenol extract that the inventive method obtains
The mensuration of fungistatic effect:
Strains tested is activated on slant medium, with inoculating loop picking ring 1-2 ring in 50mL physiological saline solution three
Vibrate in the bottle of angle 10min (inside having several beades), it is desirable to bacteria suspension about 108About CFU/mL is standby.Weigh a certain amount of vacuum
The Semen Mangiferae indicae polyphenol extract sample that cryodesiccated embodiment 4-6 obtains, with 40% aseptic ethanol on superclean bench
Aqueous dissolution sample is to required concentration, standby.Freshly prepared culture medium, based on sterilizing under the conditions of 121 DEG C, works as culture medium
When being cooled to 50-60 DEG C, on superclean bench, pour culture medium into sterilizingCulture dish in, every ware 15-20mL train
Support base, after flat board cools down, every ware adds 0.5mL bacteria suspension, uniformly paint thin plate with triangular glass painting rod standby.By aseptic
Filter paper immerses the medicinal liquid wherein 12h prepared, and drains.Each containing on bacterium flat board, 3 band medicine aseptic papers of arranging in equilateral triangle
Sheet, antibacterial is cultivated 24h, beer yeast and aspergillus niger under the conditions of 37 DEG C and cultivates 5d under the conditions of 30 DEG C, surveys its antibacterial circle diameter.
Its fungistatic effect is determined according to inhibition zone size.Be not added with the many phenol solutions of Semen Mangiferae indicae only add bacteria suspension for comparison.Result shows,
The Semen Mangiferae indicae polyphenol extract that the present invention obtains has strong fungistatic effect.
Semen Mangiferae indicae polyphenol substance antioxidant activity is studied:
Hydroxyl is the active oxidation free radical that activity is the strongest, can induce body and produce oxidative damage, and its clearance rate may often be such that
The important indicator of reaction antioxidation of drug.Same, superoxide anion is also the reactive oxygen free radical that organism is main, by
The body lipid peroxidating that it causes is body aging, cardiovascular disease and tumorigenic major reason.Semen Mangiferae indicae polyphenol hydroxyl
Base radical scavenging activity is as in figure 2 it is shown, Scavenging activity increases along with polyphenol concentration and increases, when the concentration of Semen Mangiferae indicae polyphenol reaches
About 90% can be reached to clearance rate during 0.6mg/ml, illustrate that Semen Mangiferae indicae polyphenol has stronger removing hydroxyl radical free radical effect.Awns
Pit polyphenol ultra-oxygen anion free radical Scavenging activity, as it is shown on figure 3, the concentration of Semen Mangiferae indicae polyphenol is the highest, removes superoxide anion
The ability of free radical is the strongest, when the concentration of Semen Mangiferae indicae polyphenol reaches 0.5-0.6mg/mL, and Scavenging activity tends to be steady, and clearance rate reaches
To 83%, but Scavenging activity is more weak compared with Vc.Semen Mangiferae indicae polyphenol extract DPPH radical scavenging activity result as shown in Figure 4,
Scavenging activity increases along with polyphenol concentration and increases, when concentration reaches more than 0.6mg/mL, and its removing to DPPH free radical
Rate can reach 90%, illustrates that Semen Mangiferae indicae polyphenol has stronger removing DPPH Free Radical.Data above explanation Fructus Mangifera Indicae pit
Polyphenol is respectively provided with preferable Scavenging activity to hydroxyl radical free radical and ultra-oxygen anion free radical, and result of study will provide for Semen Mangiferae indicae
Comprehensive utilization and the corresponding functional food exploitation thereof in source provide reference.
Above by embodiment, present disclosure is described in detail, but described content has been only the preferable of the present invention
Embodiment, it is impossible to be considered the practical range for limiting the present invention.Any person skilled in the art, without departing from the present invention's
In spirit and scope, all can do various changes and modification, therefore protection scope of the present invention should be with claims institute circle
Fixed is as the criterion.
Claims (10)
1. the preparation method of a Semen Mangiferae indicae polyphenol extract, it is characterised in that said method comprising the steps of:
(1) by Semen Mangiferae indicae drying and crushing；
(2) with the ethanol solution of determining alcohol 20-90%v/v or methanol solution as the solvent Semen Mangiferae indicae to obtaining in step (1)
Dried powder extracts, then by the extracting solution concentrating under reduced pressure obtained or vacuum lyophilization to extractum；
(3) the extractum water obtained in step (2), determining alcohol are less than less than the ethanol solution of 50%v/v or determining alcohol
The methanol solution of 50%v/v dissolves, and removes insoluble matter, obtains load solution；
(4) negated polarity or low pole macroporous adsorbent resin, the load solution loading that step (3) is obtained, first wash roguing with water
Matter, then carries out eluting with eluant, collects eluent；Described macroporous adsorbent resin and Semen Mangiferae indicae dried powder weight ratio are 1:
0.5-1:20；
(5) eluent concentrating under reduced pressure step (4) collected or vacuum lyophilization, obtain Semen Mangiferae indicae polyphenol extract.
Method the most according to claim 1, it is characterised in that the macroporous adsorbent resin of described step (4) is AB-8, DM21
Or D101.
Method the most according to claim 1, it is characterised in that the eluant of described step (4) is concentration 5%-95%v/v
Ethanol solution or methanol solution；The elution volume of eluant is 1-10 times of column volume.
Method the most according to claim 1, it is characterised in that the eluant of described step (4) be determining alcohol be 40-70%
Ethanol solution.
Method the most according to claim 1, it is characterised in that in described step (2), extracting method is every kilogram of Semen Mangiferae indicae
Powder adds ethanol solution or the methanol solution that 2-50L determining alcohol is 30%-80%, and hot reflux or dipping or leaching are filtered and extracted 2-5
Secondary, extract 1-3h during circumfluence distillation every time, impregnate or soak and extract 10-15 days when filtering extraction every time, united extraction liquid.
Method the most according to claim 1, it is characterised in that the extraction of described step (2) is to carry out at 40-60 DEG C,
Extraction time 1-4 time, each extraction time be 0.5-4h, solid-liquid ratio is 1:1-1:40.
7. according to the arbitrary described method of claim 1-6, it is characterised in that described step (4) specifically: take and Semen Mangiferae indicae powder
Weight ratio is the low pole macroporous resin of 1:5, the load solution loading that will obtain in step (3), uses the washing of 2-5BV to go
Impurity, rear 50% ethanol solution using 3-10BV carries out eluting, collects the eluent that the ethanol solution of 50% affords.
Method the most according to claim 1, it is characterised in that described method specifically: (1) is by Semen Mangiferae indicae drying and crushing；
(2) weigh Semen Mangiferae indicae powder 1kg adding 20L concentration is the ethanol solution of 50%, and circumfluence distillation three times, circumfluence distillation is each
1h, united extraction liquid, extracting solution concentrating under reduced pressure juice extractum；(3) the extractum water dissolution of extractum weight 10 times, filters away insoluble
Thing, obtains load solution；(4) by load solution upper prop, controlling effluent flow velocity is 0.5BV/h, applied sample amount and Resin A B-8 body
Long-pending ratio is 2:1, fully enters resin bed to extracting solution；The water using 2-5BV carries out eluting, to remove impurity, then uses 3-
10BV 40～60% ethanol solution rinse resin bed, control effluent flow velocity 2BV/h, collect ethanol solution eluent；(5)
The eluent of collection is evaporated to certain volume, is spray-dried, obtain purified product.
The Semen Mangiferae indicae polyphenol extract that method the most according to claim 1 obtains.
10. the Semen Mangiferae indicae polyphenol extract described in claim 9 is in the application prepared on food, health product or medicine.
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| CN106138130A (en) * | 2016-07-26 | 2016-11-23 | 贺州学院 | A kind of mango core chromocor extract and preparation method thereof |
| CN107348367A (en) * | 2017-06-27 | 2017-11-17 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of method that jerky antioxidant effect is improved using mulberry fruit polyphenol extract |
| CN107788531A (en) * | 2017-11-10 | 2018-03-13 | 西华大学 | A kind of method that polyphenol is extracted in the fruit stone from mango |
| AU2019236633A1 (en) * | 2019-08-28 | 2021-03-18 | Seowon University Institute of Industry - Academy Collaboration | Cosmetic composition comprising Mangifera indica irwin seed extract |
| CN112914012A (en) * | 2021-01-29 | 2021-06-08 | 广西壮族自治区农业科学院 | Extraction method and application of bonded aroma substances in mango peel |
| CN113024616A (en) * | 2021-03-22 | 2021-06-25 | 贺州学院 | Preparation method of mango seed tannic acid |
| CN113214233A (en) * | 2021-05-27 | 2021-08-06 | 贺州学院 | Method for extracting and purifying mangiferin from mango seeds |
| CN114983883A (en) * | 2022-05-06 | 2022-09-02 | 南京斯拜科生物科技股份有限公司 | Preparation process of water-soluble mango kernel extract used in cosmetics |
| CN115606734A (en) * | 2022-09-15 | 2023-01-17 | 滇西科技师范学院 | Elaeagnus conferta roxb antioxidant extract and preparation method and application thereof |
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| CN106138130A (en) * | 2016-07-26 | 2016-11-23 | 贺州学院 | A kind of mango core chromocor extract and preparation method thereof |
| CN107348367A (en) * | 2017-06-27 | 2017-11-17 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of method that jerky antioxidant effect is improved using mulberry fruit polyphenol extract |
| CN107788531A (en) * | 2017-11-10 | 2018-03-13 | 西华大学 | A kind of method that polyphenol is extracted in the fruit stone from mango |
| AU2019236633A1 (en) * | 2019-08-28 | 2021-03-18 | Seowon University Institute of Industry - Academy Collaboration | Cosmetic composition comprising Mangifera indica irwin seed extract |
| AU2019236633B2 (en) * | 2019-08-28 | 2021-05-27 | Seowon University Institute of Industry - Academy Collaboration | Cosmetic composition comprising Mangifera indica irwin seed extract |
| CN112914012A (en) * | 2021-01-29 | 2021-06-08 | 广西壮族自治区农业科学院 | Extraction method and application of bonded aroma substances in mango peel |
| CN112914012B (en) * | 2021-01-29 | 2023-09-15 | 广西壮族自治区农业科学院 | Extraction method and application of bonded aroma substances in mango peel |
| CN113024616A (en) * | 2021-03-22 | 2021-06-25 | 贺州学院 | Preparation method of mango seed tannic acid |
| CN113214233A (en) * | 2021-05-27 | 2021-08-06 | 贺州学院 | Method for extracting and purifying mangiferin from mango seeds |
| CN114983883A (en) * | 2022-05-06 | 2022-09-02 | 南京斯拜科生物科技股份有限公司 | Preparation process of water-soluble mango kernel extract used in cosmetics |
| CN115606734A (en) * | 2022-09-15 | 2023-01-17 | 滇西科技师范学院 | Elaeagnus conferta roxb antioxidant extract and preparation method and application thereof |
| CN115606734B (en) * | 2022-09-15 | 2024-04-30 | 滇西科技师范学院 | Antioxidant extract of elaeagnus conferta roxb as well as preparation method and application thereof |
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Application publication date: 20161012 Assignee: Guilin Jinsu Food Co.,Ltd. Assignor: HEZHOU University Contract record no.: X2023980046404 Denomination of invention: Mango seed polyphenol extract and its preparation method Granted publication date: 20200804 License type: Common License Record date: 20231108 Application publication date: 20161012 Assignee: Guangxi Zhulin Food Sales Co.,Ltd. Assignor: HEZHOU University Contract record no.: X2023980046401 Denomination of invention: Mango seed polyphenol extract and its preparation method Granted publication date: 20200804 License type: Common License Record date: 20231108 Application publication date: 20161012 Assignee: Zhongshan County Fuqiang Agricultural Planting Professional Cooperative Assignor: HEZHOU University Contract record no.: X2023980046398 Denomination of invention: Mango seed polyphenol extract and its preparation method Granted publication date: 20200804 License type: Common License Record date: 20231108 Application publication date: 20161012 Assignee: Guangxi Fuchuan Rongrong Agricultural Development Co.,Ltd. Assignor: HEZHOU University Contract record no.: X2023980046375 Denomination of invention: Mango seed polyphenol extract and its preparation method Granted publication date: 20200804 License type: Common License Record date: 20231108 Application publication date: 20161012 Assignee: Fuchuan Kangzhuang Breeding Family Farm Assignor: HEZHOU University Contract record no.: X2023980046366 Denomination of invention: Mango seed polyphenol extract and its preparation method Granted publication date: 20200804 License type: Common License Record date: 20231108 Application publication date: 20161012 Assignee: GUANGXI ZHONGSHAN FUQIANG YAYAO HUAXIANG AGRICULTURE CO.,LTD. Assignor: HEZHOU University Contract record no.: X2023980046356 Denomination of invention: Mango seed polyphenol extract and its preparation method Granted publication date: 20200804 License type: Common License Record date: 20231108 |