CN105527354A - Quantitative determination method of lutein content in high-fat sample - Google Patents
Quantitative determination method of lutein content in high-fat sample Download PDFInfo
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention discloses a quantitative determination method of lutein content in a high-fat sample. Cis-structure containing lutein obtained through an isomerization reaction under the action of light and iodine is taken as a reference substance, qualitative treatment is performed on cis-lutein generated through conversation in a sample determination process, correction is performed according to the proportion of trans-lutein in the total lutein peak area with a chromatographic normalization method, and the content of total lutein in the sample is acquired. Determination is performed with the method, determination error caused by cis-trans isomerism of lutein in the determination process can be avoided, the quantitative determination requirements of lutein in the aspect of precision, reproducibility, repeatability, detection limit and the like can be met, and the method is suitable for accurate determination of the lutein content in the high-fat food such as milk powder, ice cream and the like.
Description
Technical field
The invention belongs to analytical chemistry field, be specifically related to a kind of method for quantitatively determining of high fat sample Lutein content.
Background technology
Xenthophylls is a kind of dihydroxy carotenoid containing ionone ring, its structure
, molecular weight is 568.85.Xenthophylls contains two different ionone rings, β-and ε-ionone ring, the 3rd C of each ionone ring exists a functional hydroxy, at C-3, C-3 ' and C-6 ' place have three asymmetric centers, therefore have 8 kinds of steric isomers in theory.Xenthophylls has multiple cis-trans-isomer, and the main source of occurring in nature xenthophylls is extracted by marigold, and it is main existence form with trans-isomer.But xenthophylls, under the impact being subject to the effects such as illumination, heating and air oxidation, cis-trans isomerization may occur, produce the xenthophylls of cis-structure, its molecular structure is as follows:
。
European Union (Directive94/36/ECE161b) and the U.S. (21CFR73.295) all allow the xenthophylls extracted from natural plants to use as food color.Also allow to use xenthophylls in China national food safety standard GB2760 " food additives use standard " and GB14480 " food enrichment use standard " as colorant in 11 kinds of food such as milk powder, frozen, bakery product and use in part food for special foods, and for different food products, maximum use amount be 0.3mg/kg-150mg/kg not etc.Therefore, accurate quantitative analysis carries out to the xenthophylls added in food particularly important.
Because natural xenthophylls mainly exists with the form of trans-isomer, cis-content is low, separation difficulty, and does not have standard items to sell so far, and accurate quantitative analysis is had difficulties.Meanwhile, xenthophylls is liposoluble substance, as food additives or nutrition fortifier add to the higher food of fat content as in milk powder time, be easily combined with lipid material and make to extract difficulty.In addition, in xenthophylls testing process, the reason such as temperature, illumination all can make the xenthophylls generation isomerization of transconfiguration, is converted into cis xenthophylls, but conversion ratio and converted product uncertain, also make its accurate quantitative analysis be difficult to carry out.
Summary of the invention
The object of the present invention is to provide a kind of method for quantitatively determining of high fat sample Lutein content, measure by the inventive method, mensuration process Lutein cis-trans isomerization can be avoided and the error at measurment caused, in precision, reappearance, repeatability, detection limit etc., all can meet the quantitative testing requirement of xenthophylls, be applicable to the Accurate Determining of the high-fat foods such as milk powder, ice cream Lutein content.
For achieving the above object, the present invention adopts following technical scheme:
A kind of method for quantitatively determining of high fat sample Lutein content, it is the xenthophylls product in contrast containing the cis-structure isomerization reaction of light iodine obtained, carry out qualitative to the cis xenthophylls transforming generation in sample determination process, adopt chromatogram normalization method again, correct according to trans-lutein proportion in total xenthophylls peak area, obtain total lutein content in sample;
Described high-fat foods be fat content higher than the food of 3%, comprise milk powder, ice cream.
Its concrete grammar comprises the steps:
1) preparation of reference substance: take ethanol as solvent, trans-lutein standard items are mixed with the standard solution that concentration is 800 μ g/L, then by its with isocyatic iodohydrin solution by volume 1:20 mix, shake up, under daylight or daylight lamp, place 30min, obtain the xenthophylls containing cis-structure of product in contrast;
2) drafting of typical curve: get 5mg step 1) gained xenthophylls, to contain the dissolve with ethanol solution of 0.1wt%BHT and to be settled to 100mL, therefrom accurately pipette 0.050 respectively, 0.100,0.200,0.400,1.00mL is in the brown volumetric flask of 25mL, be settled to scale with the ethanolic solution containing 0.1wt%BHT, obtain the series standard working fluid that concentration is 0.100,0.200,0.400,0.800,2.00 μ g/mL; After gained standard working solution being crossed 0.45 μm of filter membrane, get subsequent filtrate and inject liquid chromatography respectively, measure corresponding peak area, then with the concentration of standard working solution for horizontal ordinate, take peak area as ordinate, drawing standard curve;
3) pre-service of sample: get 2g testing sample, adds the ethanolic solution 10mL containing 0.1wt%BHT, and mixing, adds the potassium hydroxide solution of 10mL mass concentration 10%, and vortex oscillation 1min mixes, then room temperature lucifuge vibration saponification 30min; Then the centrifugal 3min of 3min, 4500r/min is extracted with 10mL ether-normal hexane-thiacyclohexane ternary solvent system (40:40:20, v/v/v) lucifuge vortex oscillation; Merge extract after repeating extraction 2 times, add 10mL water, the centrifugal 3min of 4500r/min carries out layering washing, and merge organic phase after repeated washing 1 time, reduced pressure at room temperature is concentrated near dry;
4) assay: the ethanolic solution vortex oscillation of pretreated for step 3) sample containing 0.1wt%BHT is dissolved, and is settled to 5mL, cross 0.45 μm of filter membrane, get subsequent filtrate and carry out liquid chromatogram measuring; Adopt chromatogram normalization method, correct, then according to step 2 according to trans-lutein proportion in total xenthophylls peak area) total lutein content in gained typical curve calculation sample.
Its liquid phase chromatogram condition is: YMCCarotenoid chromatographic column, 5 μm, 250mm × 4.6mm; Column temperature: 30 DEG C; With containing 0.1wt%BHT methanol/water (88:12, v/v) ?be that mobile phase carries out gradient elution containing the methyl tert-butyl ether of 0.1wt%BHT; Flow velocity: 1.0mL/min; Determined wavelength: 445nm; Sample size: 50 μ L.
The program of gradient elution is: 0-18min, and the volume of the methanol/water containing 0.1wt%BHT in mobile phase is converted into 10% by 100%; 18.1min, is converted into 100% by the volume of the methanol/water containing 0.1wt%BHT in mobile phase by 10%, retains 10min.
Remarkable advantage of the present invention is: measure by the inventive method, mensuration process Lutein cis-trans isomerization can be avoided and the error at measurment caused, in precision, reappearance, repeatability, detection limit etc., all can meet the quantitative testing requirement of xenthophylls, be applicable to the Accurate Determining of the high-fat foods such as milk powder, ice cream Lutein content.
Accompanying drawing explanation
Fig. 1 is the liquid chromatogram of xenthophylls after the isomerization of light iodine, and wherein 1 is the xenthophylls of cis-structure, and 2 is the xenthophylls of transconfiguration.
Fig. 2 is the liquid chromatogram of milk powder Lutein, and wherein 1 is cis xenthophylls, and 2 is trans-lutein.
Embodiment
More being convenient to make content of the present invention understand, below in conjunction with embodiment, technical solutions according to the invention are described further, but the present invention being not limited only to this.
1 reagent and material
Thiacyclohexane, ether, normal hexane, absolute ethyl alcohol, methyl tert-butyl ether, dibutyl hydroxy toluene (BHT) are chromatographically pure; Potassium hydroxide, iodine are pure for analyzing; Xenthophylls (CASNo.127-40-2) standard items, purity is not less than 98.0%, with 0.1%BHT dissolve with ethanol solution and preparing standard solution.Neutral alumina solid phase extraction column, 500mg/3mL, with the drip washing of 5mL extraction solvent before using, keeps cylinder moistening.
Liquid chromatograph, band diode array detector or UV-detector; Analytical balance: sensibility reciprocal 0.01mg and 0.01g; Tissue mashing machine; Vortex oscillator; Oscillator; Vacuum concentration equipment; Solid-phase extraction device; Hydro-extractor: rotating speed is not less than 4500r/min.
2 experimental procedures
The preparation of 2.1 reference substances:
By list of references (" C30 post is separated the xenthophylls compounds pre-test in marigold flower ", Zhang Yan etc., Food Science, 2006,27 (11): 424-428) isomerization reaction of trans-lutein light iodine is utilized to prepare cis xenthophylls, the steps include: to take ethanol as solvent, trans-lutein is mixed with the standard solution that concentration is 800 μ g/L, then by its with isocyatic iodohydrin solution by volume 1:20 mix, shake up, under daylight or daylight lamp, place 30min, obtain the xenthophylls containing cis-structure of product in contrast.
The drafting of 2.2 standard solution:
Accurately take 5mg(and be accurate to 0.01mg) step 1) gained xenthophylls, to contain the dissolve with ethanol solution of 0.1wt%BHT and to be settled to 100mL, therefrom accurately pipette 0.050 respectively, 0.100,0.200,0.400,1.00mL is in the brown volumetric flask of 25mL, be settled to scale with the ethanolic solution containing 0.1wt%BHT, obtain the series standard working fluid that concentration is 0.100,0.200,0.400,0.800,2.00 μ g/mL; After gained standard working solution being crossed 0.45 μm of filter membrane, get subsequent filtrate and inject liquid chromatography respectively, measure corresponding peak area, then with the concentration of standard working solution for horizontal ordinate, take peak area as ordinate, drawing standard curve;
2.3 sample pre-treatments
Accurately taking 2g(and be accurate to 0.01g) testing sample is in 50mL polypropylene centrifuge tube, add the ethanolic solution 10mL containing 0.1wt%BHT, mixing, adds the potassium hydroxide solution of 10mL mass concentration 10%, vortex oscillation 1min mixes, then room temperature lucifuge vibration saponification 30min; Then the centrifugal 3min of 3min, 4500r/min is extracted with 10mL ether-normal hexane-thiacyclohexane ternary solvent system (40:40:20, v/v/v) lucifuge vortex oscillation; Merge extract after repeating extraction 2 times, add 10mL water, the centrifugal 3min of 4500r/min carries out layering washing, merges organic phase, be concentrated near dry in reduced pressure at room temperature after repeated washing 1 time; Dissolve with the ethanolic solution vortex oscillation containing 0.1wt%BHT and be settled to 5mL, crossing 0.45 μm of filter membrane, getting subsequent filtrate for liquid chromatogram measuring.
2.4 instrument condition
Chromatographic column: YMC
tMcarotenoid chromatographic column, 5 μm, 250mm × 4.6mm(i.d); Column temperature: 30 DEG C; Mobile phase: containing 0.1wt%BHT methanol/water (88:12, v/v) ?containing the methyl tert-butyl ether of 0.1wt%BHT, gradient elution: 0-18min, methanol/water is converted into 10% by 100%; 18.1min, methanol/water is converted into 100% by 10%, retains 10min; Flow velocity: 1.0mL/min; Determined wavelength: 445nm; Sample size: 50 μ L.
2.5 assay
Adopt chromatogram normalization method, correct, then according to step 2 according to trans-lutein proportion in total xenthophylls peak area) total lutein content in gained typical curve calculation sample, its computing formula is:
In formula:
for the content of xenthophylls total in sample, unit is μ g/100g;
for the content of cis xenthophylls in the sample liquid that obtains according to typical curve, unit is μ g/mL;
for the volume of the final constant volume of sample, unit is mL;
for sample weighting amount, unit is g;
100 is conversion coefficient;
F is correction coefficient.By obtaining with under type: use liquid-phase chromatographic analysis sample solution, using cis xenthophylls and trans-lutein chromatographic peak area adduction as total peak area, wherein trans-lutein peak area is correction coefficient divided by total peak area income value.
3 results
Fig. 1 is the liquid chromatogram of xenthophylls after the isomerization of light iodine, and wherein 1 is the xenthophylls of cis-structure, and 2 is the xenthophylls of transconfiguration.
Fig. 2 is the liquid chromatogram of milk powder Lutein, and wherein 1 is cis xenthophylls, and 2 is trans-lutein.As seen from Figure 1, the powdered milk sample containing trans-lutein can be partially converted into cis-structure in experimental implementation process.
With milk powder and ice cream for matrix, adopt the inventive method to carry out recovery test to wherein lutein content, trans-lutein adds concentration and is respectively 10,100 and 300 μ g/100g, each sample, each Pitch-based sphere replication 3 times, average, the results are shown in Table 1.
Table 1 difference adds the measurement result of concentration
In existing detection, if do not consider the cis-trans isomerization of xenthophylls during quantitative measurement, the recovery generally lower than 75%, and due to the conversion between suitable, trans-lutein be not Quantitative yield, make to detect precision not good.And visible by table 1, adopt this method to detect, its recovery is more than 80%, and relative standard deviation is less than 10%, and wherein the conversion ratio of cis xenthophylls is greatly about about 5-30%.
Further employing the inventive method detects the commercially available babies ' formula milk powder (theoretical value of this milk powder is 240 μ g/100g) that with the addition of xenthophylls, and wherein trans-lutein detected value is 201.7 μ g/100g, and total xenthophylls detected value is 215.9 μ g/100g.As can be seen here, the inventive method is convenient, stable, adopts this inventive method to carry out quantitative measurement, all can meet the quantitative testing requirement of high fat sample Lutein in precision, reappearance, repeatability, detection limit etc.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (6)
1. the method for quantitatively determining of one kind high fat sample Lutein content, it is characterized in that: the xenthophylls product in contrast containing cis-structure that the isomerization reaction of light iodine is obtained, carry out qualitative to the cis xenthophylls transforming generation in sample determination process, adopt chromatogram normalization method again, correct according to trans-lutein proportion in total xenthophylls peak area, obtain total lutein content in sample;
Described high-fat foods be fat content higher than the food of 3%, comprise milk powder, ice cream.
2. the method for quantitatively determining of high fat sample Lutein content according to claim 1, is characterized in that: its concrete grammar comprises the steps:
1) preparation of reference substance: take ethanol as solvent, trans-lutein standard items are mixed with the standard solution that concentration is 800 μ g/L, then by its with isocyatic iodohydrin solution by volume 1:20 mix, shake up, under daylight or daylight lamp, place 30min, obtain the xenthophylls containing cis-structure of product in contrast;
2) drafting of typical curve: get 5mg step 1) gained xenthophylls, to contain the dissolve with ethanol solution of 0.1wt%BHT and to be settled to 100mL, therefrom accurately pipette 0.050 respectively, 0.100,0.200,0.400,1.00mL is in the brown volumetric flask of 25mL, be settled to scale with the ethanolic solution containing 0.1wt%BHT, obtain the series standard working fluid that concentration is 0.100,0.200,0.400,0.800,2.00 μ g/mL; After respectively gained standard working solution being crossed 0.45 μm of filter membrane, get subsequent filtrate inject liquid chromatography, measure corresponding peak area, then with the concentration of standard working solution for horizontal ordinate, take peak area as ordinate, drawing standard curve;
3) pre-service of sample: get 2g testing sample, adds the ethanolic solution 10mL containing 0.1wt%BHT, and mixing, adds the potassium hydroxide solution of 10mL mass concentration 10%, and vortex oscillation 1min mixes, then room temperature lucifuge vibration saponification 30min; Then the centrifugal 3min of 3min, 4500r/min is extracted with 10mL ether-normal hexane-thiacyclohexane ternary solvent system lucifuge vortex oscillation; Merge extract after repeating extraction 2 times, add 10mL water, the centrifugal 3min of 4500r/min carries out layering washing, and merge organic phase after repeated washing 1 time, reduced pressure at room temperature is concentrated near dry;
4) assay: the ethanolic solution vortex oscillation of pretreated for step 3) sample containing 0.1wt%BHT is dissolved, and is settled to 5mL, cross 0.45 μm of filter membrane, get subsequent filtrate and carry out liquid chromatogram measuring; Adopt chromatogram normalization method, correct, then according to step 2 according to trans-lutein proportion in total xenthophylls peak area) total lutein content in gained typical curve calculation sample.
3. the method for quantitatively determining of high fat sample Lutein content according to claim 2, is characterized in that: in described ether-normal hexane-thiacyclohexane ternary solvent system, the volume ratio of ether, normal hexane and cyclohexane is 40: 40: 20.
4. the method for quantitatively determining of high fat sample Lutein content according to claim 2, is characterized in that: its liquid phase chromatogram condition is: YMCCarotenoid chromatographic column, 5 μm, 250mm × 4.6mm; Column temperature: 30 DEG C; With containing 0.1wt%BHT methyl alcohol/Shui ?carry out gradient elution containing the methyl tert-butyl ether of 0.1wt%BHT for mobile phase; Flow velocity: 1.0mL/min; Determined wavelength: 445nm; Sample size: 50 μ L.
5. the method for quantitatively determining of high fat sample Lutein content according to claim 4, is characterized in that: in described mobile phase, the volume ratio of methyl alcohol and water is 88:12.
6. the method for quantitatively determining of high fat sample Lutein content according to claim 4, is characterized in that: the program of gradient elution is: 0-18min, and the volume of the methanol/water containing 0.1wt%BHT in mobile phase is converted into 10% by 100%; 18.1min, is converted into 100% by the volume of the methanol/water containing 0.1wt%BHT in mobile phase by 10%, retains 10min.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106198818A (en) * | 2016-08-13 | 2016-12-07 | 广州白云山汉方现代药业有限公司 | A kind of quality determining method of xanthophyll preparation |
| CN108008036A (en) * | 2017-11-23 | 2018-05-08 | 广州市辉乐医药科技有限公司 | A kind of method for measuring Flos Tagetis Erectae extract Lutein content |
| CN109839453A (en) * | 2017-11-29 | 2019-06-04 | 中国科学院大连化学物理研究所 | A kind of content assaying method of microalgae carotenoid composition |
| WO2021082452A1 (en) * | 2019-10-28 | 2021-05-06 | 江苏康仁医药科技开发有限公司 | Method for determining composition of lutein |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198818A (en) * | 2016-08-13 | 2016-12-07 | 广州白云山汉方现代药业有限公司 | A kind of quality determining method of xanthophyll preparation |
| CN108008036A (en) * | 2017-11-23 | 2018-05-08 | 广州市辉乐医药科技有限公司 | A kind of method for measuring Flos Tagetis Erectae extract Lutein content |
| CN109839453A (en) * | 2017-11-29 | 2019-06-04 | 中国科学院大连化学物理研究所 | A kind of content assaying method of microalgae carotenoid composition |
| WO2021082452A1 (en) * | 2019-10-28 | 2021-05-06 | 江苏康仁医药科技开发有限公司 | Method for determining composition of lutein |
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Application publication date: 20160427 |