CN108191805A - A kind of method that natural procyanidins are extracted in the rose from polyphyll - Google Patents
A kind of method that natural procyanidins are extracted in the rose from polyphyll Download PDFInfo
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- CN108191805A CN108191805A CN201810184073.9A CN201810184073A CN108191805A CN 108191805 A CN108191805 A CN 108191805A CN 201810184073 A CN201810184073 A CN 201810184073A CN 108191805 A CN108191805 A CN 108191805A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
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Abstract
The present invention relates to a kind of methods that natural procyanidins are extracted in rose from polyphyll, including being pre-processed to fresh rose, obtain polyphyll rose raw material A；Raw material A is put into ultrasonic continuous adverse current low-temperature extraction equipment and is extracted, purified water is added in and carries out continuous countercurrent extraction, obtain extracting solution B；Liquor C is obtained, and in 5 20 DEG C of preservations by ceramic membrane filter is carried out by ceramic membrane processing equipment after extracting solution B progress prefiltrations；Liquor C is subjected to ultrafiltration or nanofiltration, molecular cut off is more than the impurity molecule of 500 10 ten thousand dalton, and obtains film essence filtered fluid D；Film essence filtered fluid D is subjected to film thickening processing using reverse osmosis membrane enrichment facility, obtains solid content as 5% 20% concentrate E；Concentrate E is freezed, obtains thickened solid F, by thickened solid F vacuum freeze dryings, obtains natural procyanidins product.It is deeper by rose procyanidine color produced by the invention, it is easy to preserve, production cost is relatively low, can be applied to field of food or cosmetic field.
Description
Technical field
The invention belongs to the side of natural procyanidins is extracted in biotechnology more particularly to a kind of rose from polyphyll
Method.
Background technology
Procyanidine is easily hydrolyzed to anthocyanidin and by illumination, moisture, air, temperature and pH value influence and face occurs
The problem of not easy to maintain caused by the variation of color.
It is existing extraction procyanidine technique based on traditional extraction mode, the applicable product color extracted compared with
Secretly, the taste without apparent rose.
Invention content
The object of the present invention is to provide a kind of methods that natural procyanidins are extracted in rose from polyphyll, which is characterized in that
Including：
Fresh rose is pre-processed, obtains polyphyll rose raw material A；
Raw material A is put into ultrasonic continuous adverse current low-temperature extraction equipment and is extracted, adds in material quality than 1：1-10 times
Purified water carries out continuous countercurrent extraction, obtains extracting solution B and roseleaf slag；Wherein, extraction conditions is：Extraction temperature 5-40
DEG C, ultrasonic power 15-100Hz, extraction time is 1-10 hours；
After extracting solution B is tentatively filtered using the strainer of 100-300 mesh, ceramic membrane mistake is carried out by ceramic membrane processing equipment
Filter obtains liquor C, and preserved at 5-20 DEG C；
Liquor C is subjected to ultrafiltration or nanofiltration, molecular cut off is more than the impurity molecule of ten thousand dalton of 500-10, and obtains
Film essence filtered fluid D；
Film essence filtered fluid D is subjected to film thickening processing using reverse osmosis membrane enrichment facility, obtaining solid content is
The concentrate E of 5%-20%；
Concentrate E at a temperature of -40 to -60 DEG C is freezed, obtains thickened solid F, thickened solid F vacuum refrigerations are done
Dry, vacuum degree obtains natural procyanidins product after 50-200mTorr, 20-30 hours.
Further, pretreatment is carried out to fresh rose to include：
Fresh rose is taken, calyx is gone to stay petal, hot pump in low temp drying is crushed to 20-60 mesh number sizes.
Further, plant type solid beverage is made in natural procyanidins product.
Further, natural plant pigment cosmetic additive agent is made in natural procyanidins product.
Compared with prior art, the beneficial effects of the invention are as follows：It solves in extraction process, due to procyanidine easily
Be hydrolyzed to anthocyanidin and by illumination, moisture, air, temperature and pH value influence and be not easy caused by the variation of color occurs
The problem of preservation, and reduce production cost；The rose procyanidine of production in the case where not changing shape, color compared with
It is deep, in brilliant violet color to darkviolet；The rose procyanidine of production can be applied to food and food additives industry, in addition to can be with
Outside as natural pigment, the also original fragrance of polyphyll rose may be used as fragrance.
Specific embodiment
The present invention is described in further detail below by specific examples of the implementation.
The present invention provides in a kind of rose from polyphyll extract natural procyanidins method, including：
1st, rose pre-processes：
Take fresh rose, calyx gone to stay petal, the drying of 20-30 degree hot pump in low temp (do so, can ensure fragrance and
Anthocyanidin quality does not degenerate), 20-60 mesh number sizes are crushed to, obtain polyphyll rose raw material A.(fresh rose-calyx is gone to stay flower
Valve-cleaning-paving disk-hot pump in low temp is dried-is crushed to meter 2-3 mm granules-aluminium foil bag hermetic package is spare).
It should be noted that in other other fields being processed using fresh goods rose as raw material, this hair can also be applied
Bright rose preprocess method, to ensure that fragrance and quality do not degenerate, the problem of to reduce mass production when needs Cold Chain Logistics.
2nd, (extraction is that rose particle feeds intake-ultrasonic continuous counter-current extraction-prefiltration-to the preparation process of procyanidine
Film essence filtering (ultrafiltration/nanofiltration)-film concentration (reverse osmosis membrane concentration)-vacuum freeze drying-receipts powder-packaging).Detailed process packet
It includes：
Extraction：
It takes in raw material A input ultrasonic continuous adverse current low-temperature extraction equipment and extracts, add in material quality than 1：1-10 times
Purified water (conductivity is less than 0.1), 5-40 DEG C of extraction temperature, ultrasonic power 15-100 hertz (Hz), continuous countercurrent extraction 1-
10 hours, obtain extracting solution B and roseleaf slag.
Prefiltration：Extracting solution B is subjected to prefiltration, is carried out after tentatively filtering by ceramics using the strainer of 100-300 mesh
Film process equipment carries out ceramic membrane filter and obtains liquor C, and in 5-20 DEG C of preservation.
Film essence filters：Liquor C is subjected to ultrafiltration or nanofiltration, molecular cut off is more than the miscellaneous of ten thousand dalton of 500-10 (Da)
Matter molecule simultaneously obtains film essence filtered fluid D.
Reverse osmosis membrane concentrates：Film essence filtered fluid D is carried out film thickening using reverse osmosis membrane enrichment facility to handle to obtain
Solid content is the concentrate E of 5%-20%.
Vacuum freeze drying：Concentrate E is freezed, cryogenic temperature is -40 to -60 DEG C and obtains thickened solid F, will
Thickened solid F carries out vacuum freeze drying, and vacuum degree is located at 50-200 millitorrs (mTorr), product G is obtained after 20-30 hours；
Receive powder and packaging：Product G is placed in clean area, and humidity is vacuum-packed in the environment of being less than 40%.
The rose procyanidine extracted by this method, color is deeper, in brilliant violet color to darkviolet, is easy to preserve, production
Cost is relatively low, and can be applied to field of food (such as rose solid beverage) or cosmetic field, (such as facial mask natural pigment and fragrance add
Add agent).
Case study on implementation 1
Pretreatment：Take fresh rose-go calyx that petal-hot pump in low temp drying is stayed (can only to do so, other ways may
Can no fragrance and allow anthocyanidin deterioration)-being crushed to 60 mesh number sizes-obtain polyphyll rose raw material A；
Extraction：It takes in raw material A input ultrasonic continuous adverse current low-temperature extraction equipment and extracts, add in material quality than 1：4 times
Purified water (conductivity be less than 0.1), 20 DEG C of extraction temperature, ultrasonic power 50 hertz (Hz), counter-current extraction 3 hours obtains
Extracting solution B and roseleaf slag.
Prefiltration：Extracting solution B is subjected to prefiltration, at after the strainer progress tentatively filtering of 200 mesh by ceramic membrane
Reason equipment carries out ceramic membrane filter and obtains liquor C, and in 5 DEG C of preservations.
Film essence filters：Liquor C is subjected to ultrafiltration or nanofiltration, molecular cut off is more than the magazine of 50000 dalton (Da)
Molecule simultaneously obtains film essence filtered fluid D.
Reverse osmosis membrane concentrates：Film essence filtered fluid D is carried out film thickening using reverse osmosis membrane enrichment facility to handle to obtain
Solid content is 15% concentrate E.
Vacuum freeze drying：Concentrate E is freezed, cryogenic temperature is -40 to -60 DEG C and obtains thickened solid F, will
Thickened solid F carries out vacuum freeze drying, and vacuum degree is located at 100mTorr, product G is obtained after 30 hours；
Receive powder and packaging：Product G is placed in clean area, and humidity is packaged into 1g/ bags of net content in the environment of being less than 40%
Packaging is prepared into plant type solid beverage.
This product is darkviolet crystal powder, and can be quickly dissolved in the water, sweet mouthfeel.
Case study on implementation 2
Pretreatment：Take fresh rose-go calyx that petal-hot pump in low temp drying is stayed (can only to do so, other ways may
Can no fragrance and allow anthocyanidin deterioration)-being crushed to 60 mesh number sizes-obtain polyphyll rose raw material A；
Extraction：It takes in raw material A input ultrasonic continuous adverse current low-temperature extraction equipment and extracts, add in material quality than 1：4 times
Purified water (conductivity be less than 0.1), 20 DEG C of extraction temperature, ultrasonic power 100 hertz (hz), counter-current extraction 2 hours obtains
Extracting solution B and roseleaf slag.
Prefiltration：Extracting solution B is subjected to prefiltration, at after the strainer progress tentatively filtering of 200 mesh by ceramic membrane
Reason equipment carries out ceramic membrane filter and obtains liquor C, and in 5 DEG C of preservations.
Film essence filters：Liquor C is subjected to ultrafiltration or nanofiltration, molecular cut off is more than the magazine of 50000 dalton (Da)
Molecule simultaneously obtains film essence filtered fluid D.
Reverse osmosis membrane concentrates：Film essence filtered fluid D is carried out film thickening using reverse osmosis membrane enrichment facility to handle to obtain
Solid content is 20% concentrate E.
Vacuum freeze drying：Concentrate E is freezed, cryogenic temperature is -40 to -60 DEG C and obtains thickened solid F, will
Thickened solid F carries out vacuum freeze drying, and vacuum degree is located at 100 milli Torr, product G is obtained after 30 hours；
Receive powder and packaging：Product G is placed in clean area, and humidity is packaged into 1kg/ bags of net content in the environment of being less than 40%
Packaging is prepared into natural plant pigment cosmetic additive agent.
This product is darkviolet crystal powder, and can be quickly dissolved in the water, and contain rose scent.
The method of industrially prepared cannabidiol provided in this embodiment has the following technical effect that：
1st, the technological gap that natural procyanidins are extracted from polyphyll rose has been filled up.
2nd, it solves in extraction process, it is empty since procyanidine is easily hydrolyzed to anthocyanidin and by illumination, moisture
Gas, temperature and pH value influence and it is not easy to maintain caused by the variation of color occurs the problem of, and reduce production cost.
3rd, it has filled up using rose as new raw-food material, the blank developed for the intensive processing of new food.
4th, the rose procyanidine produced by this method is not in the case where changing shape, and color is in brilliant violet color to dark purple
Color.
5th, it by the preprocess method of rose, solves the problems, such as to need Cold Chain Logistics during mass production, reduces life
Produce cost.
6th, the natural procyanidins of extraction can be applied to food and food additives industry, in addition to that can be used as natural colour
Element is outer, also the original fragrance of polyphyll rose, may be used as fragrance.
Those listed above is a series of to be described in detail only for feasibility embodiment of the invention specifically
Bright, they are not to limit the scope of the invention, all equivalent implementations made without departing from skill spirit of the present invention
Or change should all be included in the protection scope of the present invention.
It is obvious to a person skilled in the art that the present invention is not limited to the details of above-mentioned exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requirement rather than above description limit, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.
Claims (4)
1. the method for natural procyanidins is extracted in a kind of rose from polyphyll, which is characterized in that including：
Fresh rose is pre-processed, obtains polyphyll rose raw material A；
Raw material A is put into ultrasonic continuous adverse current low-temperature extraction equipment and is extracted, adds in material quality than 1：1-10 times of purifying
Water carries out continuous countercurrent extraction, obtains extracting solution B and roseleaf slag；Wherein, extraction conditions is：5-40 DEG C of extraction temperature surpasses
Acoustic power 15-100Hz, extraction time are 1-10 hours；
After extracting solution B is tentatively filtered using the strainer of 100-300 mesh, carry out ceramic membrane filter by ceramic membrane processing equipment and obtain
To liquor C, and preserved at 5-20 DEG C；
Liquor C is subjected to ultrafiltration or nanofiltration, molecular cut off is more than the impurity molecule of ten thousand dalton of 500-10, and obtains film essence
Filtered fluid D；
Film essence filtered fluid D is subjected to film thickening processing using reverse osmosis membrane enrichment facility, obtains solid content as 5%-
20% concentrate E；
Concentrate E at a temperature of -40 to -60 DEG C is freezed, obtains thickened solid F, by thickened solid F vacuum freeze dryings, very
Reciprocal of duty cycle is 50-200mTorr, and natural procyanidins product is obtained after 20-30 hours.
2. the method for natural procyanidins is extracted in a kind of rose from polyphyll according to claim 1, which is characterized in that institute
It states that fresh rose pre-process and includes：
Fresh rose is taken, calyx is gone to stay petal, hot pump in low temp drying is crushed to 20-60 mesh number sizes.
3. the method for natural procyanidins is extracted in a kind of rose from polyphyll according to right 1 or 2, which is characterized in that will
Plant type solid beverage is made in the natural procyanidins product.
4. the method for natural procyanidins is extracted in a kind of rose from polyphyll according to right 1 or 2, which is characterized in that its
It is characterized in that, natural plant pigment cosmetic additive agent is made in the natural procyanidins product.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810184073.9A CN108191805A (en) | 2018-03-06 | 2018-03-06 | A kind of method that natural procyanidins are extracted in the rose from polyphyll |
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| CN201810184073.9A CN108191805A (en) | 2018-03-06 | 2018-03-06 | A kind of method that natural procyanidins are extracted in the rose from polyphyll |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110346418A (en) * | 2019-07-26 | 2019-10-18 | 中国科学院西北高原生物研究所 | A method of evaluation black fruit fructus lycii quality |
| CN110432410A (en) * | 2019-08-09 | 2019-11-12 | 质每堂(武汉)健康科技有限公司 | Has the compound water plant food and drink and preparation method thereof of clearing heat and detoxicating effect |
| CN111543571A (en) * | 2020-05-26 | 2020-08-18 | 云南品斛堂生物科技有限公司 | Dendrobium nobile fermented beverage with health care function and preparation method thereof |
| CN111875573A (en) * | 2020-07-24 | 2020-11-03 | 哈工大机器人南昌智能制造研究院 | Method for continuously extracting anthocyanin from lycium ruthenicum murr |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110346418A (en) * | 2019-07-26 | 2019-10-18 | 中国科学院西北高原生物研究所 | A method of evaluation black fruit fructus lycii quality |
| CN110432410A (en) * | 2019-08-09 | 2019-11-12 | 质每堂(武汉)健康科技有限公司 | Has the compound water plant food and drink and preparation method thereof of clearing heat and detoxicating effect |
| CN111543571A (en) * | 2020-05-26 | 2020-08-18 | 云南品斛堂生物科技有限公司 | Dendrobium nobile fermented beverage with health care function and preparation method thereof |
| CN111875573A (en) * | 2020-07-24 | 2020-11-03 | 哈工大机器人南昌智能制造研究院 | Method for continuously extracting anthocyanin from lycium ruthenicum murr |
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Application publication date: 20180622 |
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