CN108184772A - A kind of semi-automatic honeybee takes ovum device and egg-fetching method - Google Patents
A kind of semi-automatic honeybee takes ovum device and egg-fetching method Download PDFInfo
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- CN108184772A CN108184772A CN201810153658.4A CN201810153658A CN108184772A CN 108184772 A CN108184772 A CN 108184772A CN 201810153658 A CN201810153658 A CN 201810153658A CN 108184772 A CN108184772 A CN 108184772A
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- sampler barrel
- liquid
- interior
- ovum
- bottom end
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention belongs to bee raising technical fields more particularly to a kind of semi-automatic honeybee to take ovum device and egg-fetching method.The device includes outer sampler barrel, interior sampler barrel, liquid supply equipment, collecting device and suction device, interior sampler barrel is socketed in outer sampler barrel, collecting device one end is connected with the air entry of suction device, the other end is connected with the top of interior sampler barrel, and liquid supply equipment is connected with the top of outer sampler barrel.This method comprises the following steps：(1) liquid is inputted into the space between outer sampler barrel and interior sampler barrel, when liquid flow to the bottom end of outer sampler barrel, outer sampler barrel is inserted into lair；(2) gas in collecting device is drawn, under the action of pressure differential, the liquid for carrying bee ovum is flow to through interior sampler barrel in collecting device.The apparatus structure is simple, easily operated, does not apply mechanical force to bee ovum to the collection process of bee ovum, ensure that the integrality of bee ovum, while also improve collection efficiency, reduce labor intensity.
Description
Technical field
The invention belongs to bee raising technical fields more particularly to a kind of semi-automatic honeybee to take ovum device and egg-fetching method.
Background technology
In the research of honeybee embryo's biology, the collection efficiency of honeybee ovum be the smooth important restriction for carrying out experiment because
Element.Since honeybee egg volume is small, chorion is fragile, slightly pressure will rupture, and the mucus of bee ovum bottom end can hinder sampling probe
Smooth transfer.
The collection mode of existing honeybee ovum is typically to use by hand picking one by one, not only cumbersome, time-consuming, laborious, but also is obtained
The ovum loss percentage taken is very high.
Invention content
(1) technical problems to be solved
The present invention is to solve the technical issues of existing bee ovum collecting mode ovum loss percentage is high.
(2) technical solution
In order to solve the above technical problem, the present invention provides a kind of semi-automatic honeybees to take ovum device, including outer sampling
Cylinder, interior sampler barrel, liquid supply equipment, collecting device and suction device, the interior sampler barrel is socketed in the outer sampler barrel, described
Collecting device one end is connected with the air entry of the suction device, and the other end is connected with the top of the interior sampler barrel, institute
The top that liquid supply equipment is stated with the outer sampler barrel is connected, and the liquid in the liquid supply equipment is inputted the outer sampler barrel
In space between interior sampler barrel.
Further, the liquid supply equipment includes pump and the solution bottle for holding the liquid, the inlet of the pump
It is connected with the solution bottle, the liquid outlet of the pump is connected with the outer sampler barrel with the space between interior sampler barrel, with
Liquid in the solution bottle is inputted into the space between the outer sampler barrel and interior sampler barrel.Preferably, solution bottle, pump and outer
It is attached between sampler barrel by silica gel hose.
Further, the pump is peristaltic pump.Peristaltic pump can adjust and control the flow velocity of liquid, so as to which liquid is made to exist
Outer sampler barrel bottom end rolls but does not flow out, and liquid can be detached and avoided from lair by bee ovum by the liquid of rolling in this way
Body largely flows into honeycomb and is adversely affected caused by honeycomb.
Preferably, outer sampler barrel and the coaxial arrangement of interior sampler barrel.The internal diameter of outer sampler barrel and interior sampler barrel is all higher than bee ovum
Size, and the respectively less than internal diameter of lair, so that carrying the liquid of bee ovum smoothly can from bottom to top flow into interior sampler barrel.
Further, the top of the interior sampler barrel is higher than the top of the outer sampler barrel, the bottom end of the interior sampler barrel
Higher than the bottom end of the outer sampler barrel.The top of interior sampler barrel is higher than the top of outer sampler barrel, both (is collected convenient for collecting device
Bottle) with the connection on interior sampler barrel top, also avoid the liquid pumped out flow into influence in sampler barrel in liquid in sampler barrel
Flowing from bottom to top；The bottom end of interior sampler barrel is higher than the bottom end of outer sampler barrel, in this way after outer sampler barrel is inserted into lair, takes outside
Bee ovum can be located at the lower section of interior sampler barrel bottom end while sample cylinder surrounds bee ovum, consequently facilitating the bottom end alignment of interior sampler barrel
Bee ovum.
Further, the bottom end of the interior sampler barrel is higher than 1~1.5mm of bottom end of the outer sampler barrel.So set, both
Bee ovum can be made to be located at the lower section of interior sampler barrel bottom end, and it is excessive apart from interior sampler barrel bottom end to be unlikely to bee ovum, is thus more convenient for
It carries in the liquid inflow of bee ovum in sampler barrel.
Further, the liquid is any one of phosphate buffer, deionized water or physiological saline, that is, ensures liquid
Body is neutrality.
Further, the suction device is air compressor machine, and the collecting device is receiving flask.Preferably, the air compressor machine,
It is attached between receiving flask and interior sampler barrel by silica gel hose.
Ovum device is taken to carry out the method for taking ovum using above-mentioned semi-automatic honeybee the present invention also provides a kind of, included successively
Following steps：
(1) liquid supply equipment is opened, the liquid is inputted into the space between the outer sampler barrel and interior sampler barrel, when
When the liquid flow to the bottom end of the outer sampler barrel, the outer sampler barrel is inserted into lair, and make the interior sampler barrel
Bottom end is directed at bee ovum；
(2) suction device is opened, to draw the gas in the collecting device, under the action of pressure differential, is carried
The liquid of the bee ovum is flow to through the interior sampler barrel in the collecting device.
Further, the step (1) is specially：The liquid supply equipment is opened, the liquid is inputted into the outer sampling
Space between cylinder and interior sampler barrel, while the flow velocity of the liquid is adjusted, so that the liquid is at the bottom of the outer sampler barrel
End rolls but does not flow out, and then the outer sampler barrel is inserted perpendicularly into lair, and the bottom end of the interior sampler barrel is made to be directed at bee
Ovum.
Further, step (3) is carried out after the step (2)：By the collecting device collect containing bee ovum
Liquid is filtered, and obtains bee ovum.
(3) advantageous effect
The above-mentioned technical proposal of the present invention has the advantages that：
The semi-automatic honeybee of the present invention takes ovum device, outer sampler barrel and interior sampler barrel as sampling probe, and for sampling when is straight
In the lair for patching bee spleen；It is defeated in space between the outside sampler barrel in top of the liquid supply equipment through outer sampler barrel and interior sampler barrel
Enter liquid, when liquid enters outer sampler barrel bottom end, you can the bee ovum in lair is impacted, so as to make bee ovum and lair point
From；At this point, the gas in collecting device is drawn using suction device, so as to which the air pressure in collecting device be reduced, in pressure differential
Under the action of, it carries bottom end of the liquid of bee ovum through interior sampler barrel and enters in collecting device, so as to fulfill the sampling to bee ovum
And collection.It is set as it can be seen that the honeybee ovum of lair bottom is removed using the tension and draught head of liquid and be delivered to collection by the device
Standby interior, entire process of collecting does not apply mechanical force to bee ovum, ensure that the integrality of bee ovum, while also improves collection effect
Rate reduces labor intensity, and package unit is simple in structure, easily operated, is very suitable for carrying out in laboratory a large amount of
The collection work of honeybee ovum.
Description of the drawings
Fig. 1 is the structure diagram that semi-automatic honeybee described in the embodiment of the present invention takes ovum device；
Wherein, 1, outer sampler barrel；2nd, interior sampler barrel；3rd, peristaltic pump；4th, solution bottle；5th, silica gel hose；6th, air compressor machine；7th, it receives
Collect bottle.
Specific embodiment
Embodiments of the present invention are described in further detail with reference to the accompanying drawings and examples.Following embodiment is used for
Illustrate the present invention, but cannot be used for limiting the scope of the invention.
In the description of the present invention, term " on ", " under ", "left", "right", " interior ", " outer ", " top ", " bottom ", " head ",
The orientation or position relationship of instructions such as " tail portions " are based on orientation shown in the drawings or position relationship, are for only for ease of description originally
It invents and simplifies description rather than indicate or imply that the device of meaning or element must have specific orientation, with specific square
Position construction and operation, therefore be not considered as limiting the invention.In addition, term " first ", " second ", " third " etc. are only used
In description purpose, and it is not intended that instruction or hint relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected or be integrally connected；It can
To be mechanical connection or be electrically connected；It can be directly connected, can also be indirectly connected by intermediary.For this
For the those of ordinary skill in field, concrete condition can be regarded and understand the concrete meaning of above-mentioned term in the present invention.
As shown in Figure 1, present embodiments providing a kind of semi-automatic honeybee takes ovum device, including outer sampler barrel 1, interior sampling
Cylinder 2, liquid supply equipment, collecting device and suction device, interior sampler barrel 2 are socketed in outer sampler barrel 1, collecting device one end and air-breathing
The air entry of equipment is connected, and the other end is connected with the top of interior sampler barrel 2, the top phase of liquid supply equipment and outer sampler barrel 1
Connection inputs the liquid in liquid supply equipment in the space between outer sampler barrel 1 and interior sampler barrel 2.
Specifically, liquid supply equipment is responsible for that liquid is made to flow in pipeline and outer sampler barrel 1, including peristaltic pump 3 and use
In the solution bottle 4 for holding liquid, the inlet of peristaltic pump 3 is connected with solution bottle 4, the liquid outlet of peristaltic pump 3 and outer sampler barrel 1
Top be connected, liquid in solution bottle 4 is inputted in the space between outer sampler barrel 1 and interior sampler barrel 2.Solution bottle 4,
It is attached between peristaltic pump 3 and outer sampler barrel 1 by the silica gel hose 5 that internal diameter is 3mm.Peristaltic pump 3 can be adjusted and be controlled
The flow velocity of liquid processed so as to which liquid is made to be rolled in outer 1 bottom end of sampler barrel but is not flowed out, can be incited somebody to action by the liquid of rolling in this way
Bee ovum is detached from lair, and liquid can be avoided largely to flow into honeycomb and adversely affected caused by honeycomb.
Liquid in liquid supply equipment can be any one of phosphate buffer, deionized water or physiological saline, that is, ensure
Liquid is neutrality；Liquid in the present embodiment is phosphate buffer.
Specifically, the suction device of the present embodiment is air compressor machine 6, collecting device is receiving flask 7.Air compressor machine 6, receiving flask
It is attached between 7 and interior sampler barrel 2 by the silica gel hose 5 that internal diameter is 3mm.
Outer sampler barrel 1 and interior sampler barrel 2 are coaxially disposed.In use, the top of interior sampler barrel 2 can be made to be higher than outer sampler barrel 1
Top, and the bottom end of interior sampler barrel 2 be higher than outer sampler barrel 1 bottom end.The top of interior sampler barrel 2 is higher than the top of outer sampler barrel 1
End both convenient for the connection on collecting device (receiving flask 7) and interior 2 top of sampler barrel, also avoids the liquid pumped out and flows into interior sample
The flowing of liquid from bottom to top in being influenced in cylinder 2 in sampler barrel 2；The bottom end of interior sampler barrel 2 is higher than the bottom end of outer sampler barrel 1,
In this way after outer sampler barrel 1 is inserted into lair, bee ovum can be located at interior 2 bottom end of sampler barrel while outer sampler barrel 1 surrounds bee ovum
Lower section, consequently facilitating interior sampler barrel 2 bottom end alignment bee ovum.
Further, in the present embodiment, the internal diameter of outer sampler barrel 1 and interior sampler barrel 2 is all higher than the size of bee ovum, and small
In the internal diameter of lair, so that carrying the liquid of bee ovum smoothly can from bottom to top flow into interior sampler barrel 2.Specific size is to take outside
The length of sample cylinder 1 and interior sampler barrel 2 is 12cm, the internal diameter of outer sampler barrel 1 for 4.5mm (about apis mellifera Linnaeus, worker bee lair
A diameter of 5.31mm, a diameter of 6.34mm of drone cell；About middle bee, a diameter of 4.61mm of worker bee lair, the lair of drone
A diameter of 5.73mm), a diameter of 3mm of interior sampler barrel 2.The bottom end of interior sampler barrel 2 is higher than the bottom end 1mm of outer sampler barrel 1.So
Setting, can not only make bee ovum be located at the lower section of interior 2 bottom end of sampler barrel, but also to be unlikely to bee ovum excessive apart from interior 2 bottom end of sampler barrel, in this way
With regard in sampler barrel 2 in the liquid inflow of easily portable bee ovum.
The present embodiment additionally provide it is a kind of ovum device is taken to carry out the method for taking ovum using above-mentioned semi-automatic honeybee, wrap successively
Include following steps：
(1) when taking ovum, bee spleen is lain on experimental bench, solution bottle 4 fills phosphate buffer, opens peristaltic pump 3, will
Liquid in solution bottle 4 inputs the space between outer sampler barrel 1 and interior sampler barrel 2, while adjusts peristaltic pump 3 and also adjust liquid
Outer sampler barrel 1 so that liquid is rolled in the bottom end of outer sampler barrel 1 but do not flowed out, is then inserted perpendicularly into lair by flow velocity by hand,
And the bottom end of interior sampler barrel 2 is made to be directed at bee ovum；
(2) air compressor machine 6 is opened, to draw the gas in collecting device, under the action of pressure differential, carries the liquid of bee ovum
It is flow in collecting device through interior sampler barrel 2；
(3) after sampling, the liquid containing bee ovum that receiving flask 7 is collected is filtered to get bee ovum.
The semi-automatic honeybee of the present embodiment takes ovum device, outer sampler barrel and interior sampler barrel as sampling probe, during for sampling
It is inserted directly into the lair of bee spleen；In space between the outside sampler barrel in top of the liquid supply equipment through outer sampler barrel and interior sampler barrel
Liquid is inputted, when liquid enters outer sampler barrel bottom end, you can the bee ovum in lair is impacted, so as to make bee ovum and lair
Separation；At this point, the gas in collecting device is drawn using suction device, so as to which the air pressure in collecting device be reduced, in pressure
Under the action of difference, carry bottom end of the liquid of bee ovum through interior sampler barrel and enter in collecting device, taken so as to fulfill to bee ovum
Sample and collection.As it can be seen that the honeybee ovum of lair bottom using the tension and draught head of liquid is removed and is delivered to collection by the device
In equipment, entire process of collecting does not apply mechanical force to bee ovum, ensure that the integrality of bee ovum, while also improves collection effect
Rate reduces labor intensity, and package unit is simple in structure, easily operated, is very suitable for carrying out in laboratory a large amount of
The collection work of honeybee ovum (worker bee and drone of apis mellifera and apis cerana).
The embodiment of the present invention provides for example and description, and is not exhaustively or by the present invention to limit
In disclosed form.Many modifications and variations are obvious for the ordinary skill in the art.Selection and
Description embodiment is to more preferably illustrate the principle of the present invention and practical application, and enables those of ordinary skill in the art
Understand the present invention so as to design the various embodiments with various modifications suitable for special-purpose.
Claims (10)
1. a kind of semi-automatic honeybee takes ovum device, which is characterized in that is set including outer sampler barrel, interior sampler barrel, liquid supply equipment, collection
Standby and suction device, the interior sampler barrel are socketed in the outer sampler barrel, described collecting device one end and the suction device
Air entry be connected, the other end is connected with the top of the interior sampler barrel, the liquid supply equipment and the outer sampler barrel
Top is connected, and the liquid in the liquid supply equipment is inputted in the space between the outer sampler barrel and interior sampler barrel.
2. semi-automatic honeybee according to claim 1 takes ovum device, which is characterized in that the liquid supply equipment includes pump and uses
In the solution bottle for holding the liquid, the inlet of the pump is connected with the solution bottle, the liquid outlet of the pump with it is described
Space between outer sampler barrel and interior sampler barrel is connected.
3. semi-automatic honeybee according to claim 2 takes ovum device, which is characterized in that the pump is peristaltic pump.
4. semi-automatic honeybee according to claim 1 takes ovum device, which is characterized in that the top of the interior sampler barrel is higher than
The top of the outer sampler barrel, the bottom end of the interior sampler barrel are higher than the bottom end of the outer sampler barrel.
5. semi-automatic honeybee according to claim 4 takes ovum device, which is characterized in that the bottom end of the interior sampler barrel is higher than
1~1.5mm of bottom end of the outer sampler barrel.
6. semi-automatic honeybee according to claim 1 takes ovum device, which is characterized in that the liquid is phosphate-buffered
Any one of liquid, deionized water or physiological saline.
7. semi-automatic honeybee according to claim 1 takes ovum device, which is characterized in that the suction device is air compressor machine,
The collecting device is receiving flask.
8. the method for ovum device being taken to carry out taking ovum using any one of the claim 1~7 semi-automatic honeybee, which is characterized in that
It in turn includes the following steps：
(1) liquid supply equipment is opened, the liquid is inputted into the space between the outer sampler barrel and interior sampler barrel, when described
When liquid flow to the bottom end of the outer sampler barrel, the outer sampler barrel is inserted into lair, and make the bottom end of the interior sampler barrel
It is directed at bee ovum；
(2) suction device is opened, to draw the gas in the collecting device, under the action of pressure differential, described in carrying
The liquid of bee ovum is flow to through the interior sampler barrel in the collecting device.
9. according to the method described in claim 8, it is characterized in that, the step (1) is specially：The liquid supply equipment is opened,
The liquid is inputted into the space between the outer sampler barrel and interior sampler barrel, while adjust the flow velocity of the liquid, so that institute
It states liquid to roll but do not flow out in the bottom end of the outer sampler barrel, is then inserted perpendicularly into the outer sampler barrel in lair, and make
The bottom end alignment bee ovum of the interior sampler barrel.
10. according to the method described in claim 8, it is characterized in that, step (3) is carried out after the step (2)：By described in
The liquid containing bee ovum that collecting device is collected is filtered, and obtains bee ovum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810153658.4A CN108184772B (en) | 2018-02-22 | 2018-02-22 | Semi-automatic bee egg taking device and egg taking method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810153658.4A CN108184772B (en) | 2018-02-22 | 2018-02-22 | Semi-automatic bee egg taking device and egg taking method |
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| CN108184772A true CN108184772A (en) | 2018-06-22 |
| CN108184772B CN108184772B (en) | 2023-05-26 |
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| CN201810153658.4A Active CN108184772B (en) | 2018-02-22 | 2018-02-22 | Semi-automatic bee egg taking device and egg taking method |
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Cited By (1)
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| Publication number | Publication date |
|---|---|
| CN108184772B (en) | 2023-05-26 |
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