CN207969568U - A kind of semi-automatic honeybee takes ovum device - Google Patents
A kind of semi-automatic honeybee takes ovum device Download PDFInfo
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- CN207969568U CN207969568U CN201820262056.8U CN201820262056U CN207969568U CN 207969568 U CN207969568 U CN 207969568U CN 201820262056 U CN201820262056 U CN 201820262056U CN 207969568 U CN207969568 U CN 207969568U
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- sampler barrel
- interior
- liquid
- ovum
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Abstract
The utility model belongs to bee raising technical field more particularly to a kind of semi-automatic honeybee takes ovum device.The device includes outer sampler barrel, interior sampler barrel, liquid supply equipment, collecting device and suction device, the interior sampler barrel is socketed in the outer sampler barrel, described collecting device one end is connected with the air entry of the suction device, the other end is connected with the top of the interior sampler barrel, the liquid supply equipment is connected with the top of the outer sampler barrel, and the liquid in the liquid supply equipment is inputted in the space between the outer sampler barrel and interior sampler barrel.The apparatus structure is simple, easily operated, does not apply mechanical force to bee ovum to the collection process of bee ovum, ensure that the integrality of bee ovum, while also improving collection efficiency, reduce labor intensity.
Description
Technical field
The utility model belongs to bee raising technical field more particularly to a kind of semi-automatic honeybee takes ovum device.
Background technology
In the research of honeybee embryo's biology, the collection efficiency of honeybee ovum be the smooth important restriction for carrying out experiment because
Element.Since honeybee egg volume is small, chorion is fragile, slightly pressure will rupture, and the mucus of bee ovum bottom end can hinder sampling probe
Smooth transfer.
The collection mode of existing honeybee ovum is typically to use by hand picking one by one, not only cumbersome, time-consuming, laborious, but also is obtained
The ovum loss percentage taken is very high.
Utility model content
(1) technical problems to be solved
To be solved in the utility model is the high technical problem of existing bee ovum collecting mode ovum loss percentage.
(2) technical solution
In order to solve the above-mentioned technical problem, the utility model provides a kind of semi-automatic honeybee and takes ovum device comprising outer
Sampler barrel, interior sampler barrel, liquid supply equipment, collecting device and suction device, the interior sampler barrel are socketed in the outer sampler barrel,
Described collecting device one end is connected with the air entry of the suction device, and the other end is connected with the top of the interior sampler barrel
Logical, the liquid supply equipment is connected with the top of the outer sampler barrel, and the liquid input in the liquid supply equipment is described outer
In space between sampler barrel and interior sampler barrel.
Further, the liquid supply equipment includes pump and the solution bottle for holding the liquid, the inlet of the pump
It is connected with the solution bottle, the liquid outlet of the pump is connected with the outer sampler barrel with the space between interior sampler barrel, with
Liquid in the solution bottle is inputted into the space between the outer sampler barrel and interior sampler barrel.Preferably, solution bottle, pump and outer
It is attached by silica gel hose between sampler barrel.
Further, the pump is peristaltic pump.Peristaltic pump can adjust and control the flow velocity of liquid, so as to make liquid exist
Outer sampler barrel bottom end rolls but does not flow out, and can be detached bee ovum and liquid from lair by the liquid of rolling in this way
Body largely flows into honeycomb and is adversely affected caused by honeycomb.
Preferably, outer sampler barrel and the coaxial arrangement of interior sampler barrel.The internal diameter of outer sampler barrel and interior sampler barrel is all higher than bee ovum
Size, and the internal diameter of respectively less than lair, so that carrying the liquid of bee ovum smoothly can from bottom to top flow into interior sampler barrel.
Further, the top of the interior sampler barrel is higher than the top of the outer sampler barrel, the bottom end of the interior sampler barrel
Higher than the bottom end of the outer sampler barrel.The top of interior sampler barrel is higher than the top of outer sampler barrel, both collecting device is convenient for (to collect
Bottle) with the connection on interior sampler barrel top, also avoid the liquid pumped out flow into influence in sampler barrel in liquid in sampler barrel
Flowing from bottom to top；The bottom end of interior sampler barrel is higher than the bottom end of outer sampler barrel, in this way after outer sampler barrel is inserted into lair, takes outside
Bee ovum can be located at the lower section of interior sampler barrel bottom end while sample cylinder surrounds bee ovum, consequently facilitating the bottom end alignment of interior sampler barrel
Bee ovum.
Further, the bottom end of the interior sampler barrel is higher than 1~1.5mm of bottom end of the outer sampler barrel.So set, both
Bee ovum can be made to be located at the lower section of interior sampler barrel bottom end, and it is excessive apart from interior sampler barrel bottom end to be unlikely to bee ovum, is thus more convenient for
The liquid for carrying bee ovum flows into interior sampler barrel.
Further, the liquid is any one of phosphate buffer, deionized water or physiological saline, that is, ensures liquid
Body is neutrality.
Further, the suction device is air compressor machine, and the collecting device is receiving flask.Preferably, the air compressor machine,
It is attached by silica gel hose between receiving flask and interior sampler barrel.
The utility model additionally provides a kind of method for taking ovum device to carry out taking ovum using above-mentioned semi-automatic honeybee, successively
Include the following steps：
(1) liquid supply equipment is opened, the liquid is inputted into the space between the outer sampler barrel and interior sampler barrel, when
When the liquid flow to the bottom end of the outer sampler barrel, the outer sampler barrel is inserted into lair, and make the interior sampler barrel
Bottom end is directed at bee ovum；
(2) suction device is opened, to draw the gas in the collecting device, under the action of pressure difference, is carried
The liquid of the bee ovum is flow to through the interior sampler barrel in the collecting device.
Further, the step (1) is specially：The liquid supply equipment is opened, the liquid is inputted into the outer sampling
Space between cylinder and interior sampler barrel, while the flow velocity of the liquid is adjusted, so that the liquid is at the bottom of the outer sampler barrel
End rolls but does not flow out, and then the outer sampler barrel is inserted perpendicularly into lair, and the bottom end of the interior sampler barrel is made to be directed at bee
Ovum.
Further, step (3) is carried out after the step (2)：By the collecting device collect containing bee ovum
Liquid is filtered, and obtains bee ovum.
(3) advantageous effect
The above-mentioned technical proposal of the utility model has the advantages that：
The semi-automatic honeybee of the utility model takes ovum device, and outer sampler barrel and interior sampler barrel are as sampling probe, for sampling
When be inserted directly into the lair of bee spleen；Space between the outside sampler barrel in top of the liquid supply equipment through outer sampler barrel and interior sampler barrel
Interior input liquid, when liquid enters outer sampler barrel bottom end, you can impacted to the bee ovum in lair, to make bee ovum and nest
Room detaches；At this point, drawing the gas in collecting device using suction device, to reduce the air pressure in collecting device, pressing
Under the action of force difference, the liquid for carrying bee ovum enters through the bottom end of interior sampler barrel in collecting device, to realize to bee ovum
Sampling and collection.As it can be seen that the honeybee ovum of lair bottom is removed using the tension and draught head of liquid and is delivered to receipts by the device
Collect in equipment, entire process of collecting does not apply mechanical force to bee ovum, ensure that the integrality of bee ovum, while also improving collection
Efficiency reduces labor intensity, and package unit is simple in structure, easily operated, is very suitable for carrying out in laboratory big
Measure the collection work of honeybee ovum.
Description of the drawings
Fig. 1 is the structural schematic diagram that semi-automatic honeybee takes ovum device described in the utility model embodiment；
Wherein, 1, outer sampler barrel；2, interior sampler barrel；3, peristaltic pump；4, solution bottle；5, silica gel hose；6, air compressor machine；7, it receives
Collect bottle.
Specific implementation mode
The embodiment of the utility model is described in further detail with reference to the accompanying drawings and examples.Following embodiment
For illustrating the utility model, but cannot be used for limiting the scope of the utility model.
Term "upper", "lower", "left", "right", "inner", "outside", "top", "bottom", " head in the description of the present invention,
The orientation or positional relationship of the instructions such as portion ", " tail portion " is to be based on the orientation or positional relationship shown in the drawings, and is merely for convenience of retouching
State the utility model and simplify and describe, do not indicate or imply the indicated device or element must have a particular orientation, with
Specific azimuth configuration and operation, therefore should not be understood as limiting the present invention.In addition, term " first ", " second ",
" third " etc. is used for description purposes only, and is not understood to indicate or imply relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, can also be electrical connection；It can be directly connected, it can also be indirectly connected through an intermediary.
For the ordinary skill in the art, it can understand that above-mentioned term in the present invention specific contains depending on concrete condition
Justice.
As shown in Figure 1, present embodiments providing a kind of semi-automatic honeybee takes ovum device comprising outer sampler barrel 1, interior sampling
Cylinder 2, liquid supply equipment, collecting device and suction device, interior sampler barrel 2 are socketed in outer sampler barrel 1, collecting device one end and air-breathing
The air entry of equipment is connected, and the other end is connected with the top of interior sampler barrel 2, the top phase of liquid supply equipment and outer sampler barrel 1
Connection inputs the liquid in liquid supply equipment in the space between outer sampler barrel 1 and interior sampler barrel 2.
Specifically, liquid supply equipment is responsible for that liquid is made to flow in pipeline and outer sampler barrel 1 comprising peristaltic pump 3 and use
It is connected with solution bottle 4 in the inlet of the solution bottle 4 for holding liquid, peristaltic pump 3, the liquid outlet of peristaltic pump 3 and outer sampler barrel 1
Top be connected, liquid in solution bottle 4 is inputted in the space between outer sampler barrel 1 and interior sampler barrel 2.Solution bottle 4,
It is attached by the silica gel hose 5 that internal diameter is 3mm between peristaltic pump 3 and outer sampler barrel 1.Peristaltic pump 3 can be adjusted and be controlled
The flow velocity of liquid processed can be incited somebody to action so as to make liquid be rolled in 1 bottom end of outer sampler barrel but do not flow out by the liquid of rolling in this way
Bee ovum is detached from lair, and avoidable liquid largely flows into honeycomb and adversely affected caused by honeycomb.
Liquid in liquid supply equipment can be any one of phosphate buffer, deionized water or physiological saline, that is, ensure
Liquid is neutrality；Liquid in the present embodiment is phosphate buffer.
Specifically, the suction device of the present embodiment is air compressor machine 6, collecting device is receiving flask 7.Air compressor machine 6, receiving flask
It is attached by the silica gel hose 5 that internal diameter is 3mm between 7 and interior sampler barrel 2.
Outer sampler barrel 1 and interior sampler barrel 2 are coaxially disposed.In use, the top of interior sampler barrel 2 can be made to be higher than outer sampler barrel 1
Top, and the bottom end of interior sampler barrel 2 be higher than outer sampler barrel 1 bottom end.The top of interior sampler barrel 2 is higher than the top of outer sampler barrel 1
The connection of collecting device (receiving flask 7) and 2 top of interior sampler barrel is both convenient at end, also avoids the liquid pumped out and flows into interior sampling
The flowing of liquid from bottom to top in being influenced in cylinder 2 in sampler barrel 2；The bottom end of interior sampler barrel 2 is higher than the bottom end of outer sampler barrel 1,
In this way after outer sampler barrel 1 is inserted into lair, bee ovum can be located at 2 bottom end of interior sampler barrel while outer sampler barrel 1 surrounds bee ovum
Lower section, consequently facilitating interior sampler barrel 2 bottom end be aligned bee ovum.
Further, in this embodiment the internal diameter of outer sampler barrel 1 and interior sampler barrel 2 is all higher than the size of bee ovum, and it is small
In the internal diameter of lair, so that carrying the liquid of bee ovum smoothly can from bottom to top flow into interior sampler barrel 2.Specific size is to take outside
The length of sample cylinder 1 and interior sampler barrel 2 is 12cm, the internal diameter of outer sampler barrel 1 be 4.5mm (about apis mellifera Linnaeus, worker bee lair
A diameter of 5.31mm, a diameter of 6.34mm of drone cell；About middle bee, a diameter of 4.61mm of worker bee lair, the lair of drone
A diameter of 5.73mm), a diameter of 3mm of interior sampler barrel 2.The bottom end of interior sampler barrel 2 is higher than the bottom end 1mm of outer sampler barrel 1.So
Setting, can not only make bee ovum be located at the lower section of 2 bottom end of interior sampler barrel, but also to be unlikely to bee ovum excessive apart from 2 bottom end of interior sampler barrel, in this way
The liquid of easily portable bee ovum is flowed into interior sampler barrel 2.
The present embodiment additionally provides a kind of method for taking ovum device to carry out taking ovum using above-mentioned semi-automatic honeybee, wraps successively
Include following steps：
(1) when taking ovum, bee spleen is lain on experimental bench, solution bottle 4 fills phosphate buffer, opens peristaltic pump 3, will
Liquid in solution bottle 4 inputs the space between outer sampler barrel 1 and interior sampler barrel 2, while adjusting peristaltic pump 3 and also adjusting liquid
Then outer sampler barrel 1 is inserted perpendicularly into lair by flow velocity by hand so that liquid is rolled in the bottom end of outer sampler barrel 1 but do not flowed out,
And the bottom end of interior sampler barrel 2 is made to be directed at bee ovum；
(2) air compressor machine 6 is opened, to draw the gas in collecting device, under the action of pressure difference, carries the liquid of bee ovum
It is flow in collecting device through interior sampler barrel 2；
(3) after sampling, the liquid containing bee ovum that receiving flask 7 is collected is filtered to get bee ovum.
The semi-automatic honeybee of the present embodiment takes ovum device, and outer sampler barrel and interior sampler barrel are as sampling probe, when for sampling
It is inserted directly into the lair of bee spleen；In space between the outside sampler barrel in top of the liquid supply equipment through outer sampler barrel and interior sampler barrel
Liquid is inputted, when liquid enters outer sampler barrel bottom end, you can impacted to the bee ovum in lair, to make bee ovum and lair
Separation；At this point, the gas in collecting device is drawn using suction device, to reduce the air pressure in collecting device, in pressure
Under the action of difference, the liquid for carrying bee ovum enters through the bottom end of interior sampler barrel in collecting device, is taken to bee ovum to realize
Sample and collection.As it can be seen that the honeybee ovum of lair bottom is removed using the tension and draught head of liquid and is delivered to collection by the device
In equipment, entire process of collecting does not apply mechanical force to bee ovum, ensure that the integrality of bee ovum, while also improving collection effect
Rate reduces labor intensity, and package unit is simple in structure, easily operated, is very suitable for carrying out in laboratory a large amount of
The collection work of honeybee ovum (worker bee and drone of apis mellifera and apis cerana).
The embodiments of the present invention provide for example and description, and are not exhaustively or by this reality
It is limited to disclosed form with novel.Many modifications and variations are apparent for the ordinary skill in the art
's.Selection and description embodiment are and to make the general of this field to more preferably illustrate the principles of the present invention and practical application
Logical technical staff is it will be appreciated that various embodiments with various modifications of the utility model to design suitable for special-purpose.
Claims (7)
1. a kind of semi-automatic honeybee takes ovum device, which is characterized in that set including outer sampler barrel, interior sampler barrel, liquid supply equipment, collection
Standby and suction device, the interior sampler barrel are socketed in the outer sampler barrel, described collecting device one end and the suction device
Air entry be connected, the other end is connected with the top of the interior sampler barrel, the liquid supply equipment and the outer sampler barrel
Top is connected, and the liquid in the liquid supply equipment is inputted in the space between the outer sampler barrel and interior sampler barrel.
2. semi-automatic honeybee according to claim 1 takes ovum device, which is characterized in that the liquid supply equipment includes pump and uses
In the solution bottle for holding the liquid, the inlet of the pump is connected with the solution bottle, the liquid outlet of the pump with it is described
Space between outer sampler barrel and interior sampler barrel is connected.
3. semi-automatic honeybee according to claim 2 takes ovum device, which is characterized in that the pump is peristaltic pump.
4. semi-automatic honeybee according to claim 1 takes ovum device, which is characterized in that the top of the interior sampler barrel is higher than
The top of the outer sampler barrel, the bottom end of the interior sampler barrel are higher than the bottom end of the outer sampler barrel.
5. semi-automatic honeybee according to claim 4 takes ovum device, which is characterized in that the bottom end of the interior sampler barrel is higher than
1~1.5mm of bottom end of the outer sampler barrel.
6. semi-automatic honeybee according to claim 1 takes ovum device, which is characterized in that the liquid is phosphate-buffered
Any one of liquid, deionized water or physiological saline.
7. semi-automatic honeybee according to claim 1 takes ovum device, which is characterized in that the suction device is air compressor machine,
The collecting device is receiving flask.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820262056.8U CN207969568U (en) | 2018-02-22 | 2018-02-22 | A kind of semi-automatic honeybee takes ovum device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820262056.8U CN207969568U (en) | 2018-02-22 | 2018-02-22 | A kind of semi-automatic honeybee takes ovum device |
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| Publication Number | Publication Date |
|---|---|
| CN207969568U true CN207969568U (en) | 2018-10-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820262056.8U Expired - Fee Related CN207969568U (en) | 2018-02-22 | 2018-02-22 | A kind of semi-automatic honeybee takes ovum device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184772A (en) * | 2018-02-22 | 2018-06-22 | 中国农业科学院蜜蜂研究所 | A kind of semi-automatic honeybee takes ovum device and egg-fetching method |
-
2018
- 2018-02-22 CN CN201820262056.8U patent/CN207969568U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184772A (en) * | 2018-02-22 | 2018-06-22 | 中国农业科学院蜜蜂研究所 | A kind of semi-automatic honeybee takes ovum device and egg-fetching method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181016 Termination date: 20190222 |