CN108174969A - A kind of method that thermal-reactive pet phagostimulant is prepared using swine blood fermentation - Google Patents
A kind of method that thermal-reactive pet phagostimulant is prepared using swine blood fermentation Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/24—Animal feeding-stuffs from material of animal origin from blood
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
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Abstract
The invention discloses a kind of method that thermal-reactive pet phagostimulant is prepared using swine blood fermentation, step is as follows:Take the swine blood fermentation enzymolysis liquid of raw material gross mass 20 80%, the reduced sugar of raw material gross mass 0.5 6%, the amino acid of raw material gross mass 0.5 6%, raw material gross mass 2 5% yeast extract, after mixing plus water complements to 100%, carry out thermal response, temperature is 100 110 DEG C, reaction time is 1 3h, it is subsequently cooled to room temperature, you can obtain thermal-reactive pet phagostimulant.Microbial fermentation technology is combined by this method with Maillard reaction, breaks down proteins in blood are converted into small-molecule substance, improve its digestive utilization ratio, and eliminate the smell of blood, using Maillard reaction flavouring, the flavor of fermentation blood can be improved, so as to improve its palatability, enhance the acceptance of pet;It is sprayed in the ratio of pet food total weight 1%~5%, the flavor and palatability of pet food can be effectively improved.
Description
Technical field
The invention belongs to food processing technology fields, especially a kind of to prepare thermal-reactive pet food calling using swine blood fermentation
The method of agent.
Background technology
Protein content reaches 19%~21% in fresh pig blood, but also contains a large amount of nonprotein nitrogen, vitamin, nothing
The many kinds of substance such as machine salt have very high utility value, and external utilized to it compares attention, study more deep, however China
Research it is less, product is single, level of processing is not high.Just start modern biotechnology being applied to pig blood deep processing in recent years
Research, has developed a series of products such as fermented blood meal, feed addictive plasma protein powder.
Fermented blood meal is to mix animal blood with certain proportion with the auxiliary material of good permeability, accesses specified strain, accumulation
It ferments, then the product of drying and crushing.Blood is free of the smell of blood after everfermentation, but has bent fragrance, wine flavour, certain pets
Poor to this flavor acceptance, fermentation blood drying process can also have flavor certain influence, can also influence palatability, and technique consumes
Can be big, its application in pet food is limited, therefore, the flavor and palatability for how improving fermentation blood are pet foods
Industry Demanding solves the problems, such as.
By retrieval, not yet find and the relevant patent publication us of present patent application.
Invention content
It is an object of the invention in place of overcome the deficiencies in the prior art, provide a kind of utilization swine blood fermentation to prepare thermal response
Microbial fermentation technology is combined by the method for type pet phagostimulant, this method with Maillard reaction, by protein in blood point
Solution is converted into small-molecule substance, improves its digestive utilization ratio, and eliminate the smell of blood, using Maillard reaction flavouring, Ke Yigai
The flavor of kind fermentation blood, so as to improve its palatability, enhances the acceptance of pet;When in use, by pet food total weight
1%~5% ratio spraying can effectively improve the flavor and palatability of pet food.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of method that thermal-reactive pet phagostimulant is prepared using swine blood fermentation, step are as follows:
Swine blood fermentation enzymolysis liquid, reduced sugar, the raw material of raw material gross mass 0.5-6% for taking raw material gross mass 20-80% are total
The amino acid of quality 0.5-6%, the yeast extract of raw material gross mass 2-5%, after mixing plus water complements to 100%, carries out heat
Reaction, temperature are 100-110 DEG C, and reaction time 1-3h is subsequently cooled to room temperature, you can obtains thermal-reactive pet food calling
Agent.
Moreover, the mixture that the reduced sugar is glucose, one or more kinds of in xylose, ribose.
Moreover, the reduced sugar is glucose, two or more mixture in xylose, ribose.
Moreover, the amino acid is glycine, alanine, l-methionine, aspartic acid, arginine, L-cysteine
Two or more mixture in hydrochloride.
Moreover, the preparation method of the swine blood fermentation enzymolysis liquid is as follows:
(1) ferment
Pig blood with wheat bran, corn flour is mixed, is stirred evenly, as culture medium, the wherein dosage of pig blood is gross mass
40%~80%, the mass ratio of wheat bran and corn flour is 1:2, it sterilizes, temperature is 121 DEG C, and time 20min is cooled to
Room temperature, inoculated aspergillus niger, aspergillus niger inoculating spores powder weight are 0.02~0.1g/g culture mediums, and every gram of conidia powder contains 108A spore
Son, 30~40 DEG C, 12~36h of fermentation time of fermentation temperature add the water of pig blood weight 1/3, and stirring 1 time, secondary temperature elevation, until 40
~45 DEG C, 24~48h is kept, obtains swine blood fermentation;
(2) digest
The swine blood fermentation of step (1) is taken, is crushed, adds in the water of swine blood fermentation identical weight, crosses colloid mill, homogenate makes
PH to 6.0~7.0 is adjusted with 1mol/L sodium hydroxide solutions or 1mol/L hydrochloric acid solutions, addition 0.1~1% is (with swine blood fermentation
Weight meter) papain and flavor protease, papain:The mass ratio of flavor protease is 2:1,1~3h is kept,
90~100 DEG C are warming up to, keeps 5~15min to get swine blood fermentation enzymolysis liquid.
The advantages of present invention obtains and good effect are:
1st, microbial fermentation technology is combined by the present invention with Maillard reaction, breaks down proteins in blood is converted into small
Molecular substance improves its digestive utilization ratio, and eliminates the smell of blood, using Maillard reaction flavouring, can improve fermentation blood
Flavor, so as to improve its palatability, enhance the acceptance of pet;When in use, by pet food total weight 1%~5%
Ratio sprays thermal-reactive pet phagostimulant made from the method for the present invention, can effectively improve the flavor of pet food and agreeable to the taste
Property.
2nd, for the method for the present invention using pig blood as primary raw material, product cost is relatively low, effectively increases the utilization rate of pig blood and attached
It is value added, avoid the waste of this high-quality resource of pig blood.
3rd, using secondary temperature elevation zymotechnique, the macro-molecular protein in the pig blood that can effectively degrade increases the method for the present invention
Free amino acid and small peptide substance in swine blood fermentation, nutrition is more comprehensive, and avoid during one time fermentation due to
Fermentation materials surface oxygen is sufficient, and hyphae length concentrates on surface layer, causes internal oxygen supply insufficient, so as to reduce the degradation of pig blood
Speed influences the quality of swine blood fermentation.
Specific embodiment
The embodiment of the present invention is described in detail below, it should be noted that the present embodiment is narrative, is not limited
, it is impossible to protection scope of the present invention is limited with this.
Raw material used in the present invention is conventional commercial product unless otherwise specified;Used in the present invention
Method is the conventional method of this field unless otherwise specified.
Embodiment 1
A kind of method that thermal-reactive pet phagostimulant is prepared using swine blood fermentation, step are as follows:
(1) swine blood fermentation enzymolysis liquid is prepared:Pig blood with wheat bran, corn flour is mixed, is stirred evenly, as culture medium,
The dosage of middle pig blood is the 40% of gross mass, and the mass ratio of wheat bran and corn flour is 1:2, it sterilizes, temperature is 121 DEG C, when
Between for 20min, be cooled to room temperature, inoculated aspergillus niger, aspergillus niger inoculating spores powder weight is 0.05g/g culture mediums (every gram of spore
Powder contains 108A spore), 30 DEG C, fermentation time 36h of fermentation temperature adds the water of pig blood weight 1/3, and stirring 1 time, secondary temperature elevation,
To 45 DEG C, keep for 24 hours, obtaining swine blood fermentation, and crush, add in the water of swine blood fermentation identical weight, cross colloid mill, it is even
Slurry adjusts pH to 6.0~7.0 using 1mol/L sodium hydroxide solutions or 1mol/L hydrochloric acid solutions, and addition 0.1% is (with fermented-pig
Blood weight meter) papain and flavor protease (mass ratio of the two is 2:1) 3h, is kept, is warming up to 100 DEG C, is protected
5min is held to get swine blood fermentation enzymolysis liquid.
(2) thermal response:Take swine blood fermentation enzymolysis liquid 50%, glucose 5%, ribose 1%, the alanine in step (1)
1%th, aspartic acid 1%, arginase 12 %, yeast extract 5%, after mixing plus water complements to 100%, carries out thermal response, temperature
It it is 100 DEG C, reaction time 3h is subsequently cooled to room temperature, you can obtain thermal-reactive pet phagostimulant.
Embodiment 2
A kind of method that thermal-reactive pet phagostimulant is prepared using swine blood fermentation, step are as follows:
(1) swine blood fermentation enzymolysis liquid is prepared:Pig blood with wheat bran, corn flour is mixed, is stirred evenly, as culture medium,
The dosage of middle pig blood is the 80% of gross mass, and the mass ratio of wheat bran and corn flour is 1:2, it sterilizes, temperature is 121 DEG C, when
Between for 20min, be cooled to room temperature, inoculated aspergillus niger, aspergillus niger inoculating spores powder weight is 0.08g/g culture mediums (every gram of spore
Powder contains 108A spore), 35 DEG C, fermentation time 18h of fermentation temperature adds the water of pig blood weight 1/3, and stirring 1 time, secondary temperature elevation,
To 40 DEG C, 48h being kept, obtains swine blood fermentation, and crush, the water for adding in swine blood fermentation identical weight crosses colloid mill, is homogenized,
PH to 6.0~7.0 is adjusted using 1mol/L sodium hydroxide solutions or 1mol/L hydrochloric acid solutions, addition 0.5% is (with swine blood fermentation weight
Gauge) papain and flavor protease (mass ratio of the two is 2:1) 1h, is kept, is warming up to 95 DEG C, is kept
10min is to get swine blood fermentation enzymolysis liquid.
(2) thermal response:Take swine blood fermentation enzymolysis liquid 60%, glucose 2%, xylose 2%, the half Guang ammonia of L- in step (1)
Acid hydrochloride 1.5%, aspartic acid 2%, alanine 1%, yeast extract 4%, after mixing plus water complements to 100%, carries out
Thermal response, temperature are 105 DEG C, and reaction time 2h is subsequently cooled to room temperature, you can obtains thermal-reactive pet phagostimulant.
Embodiment 3
A kind of method that thermal-reactive pet phagostimulant is prepared using swine blood fermentation, step are as follows:
Swine blood fermentation enzymolysis liquid, reduced sugar, the raw material of raw material gross mass 0.5-6% for taking raw material gross mass 20-80% are total
The amino acid of quality 0.5-6%, the yeast extract of raw material gross mass 2-5%, after mixing plus water complements to 100%, carries out heat
Reaction, temperature are 100-110 DEG C, and reaction time 1-3h is subsequently cooled to room temperature, you can obtains thermal-reactive pet food calling
Agent.
More preferably, the reduced sugar is two or more mixture in glucose, xylose, ribose.
More preferably, the amino acid is glycine, alanine, l-methionine, aspartic acid, arginine, half Guang ammonia of L-
Two or more mixture in acid hydrochloride.
More preferably, the preparation method of the swine blood fermentation enzymolysis liquid is as follows:
(1) ferment
Pig blood with wheat bran, corn flour is mixed, is stirred evenly, as culture medium, the wherein dosage of pig blood is gross mass
40%~80%, the mass ratio of wheat bran and corn flour is 1:2, it sterilizes, temperature is 121 DEG C, and time 20min is cooled to
Room temperature, inoculated aspergillus niger, aspergillus niger inoculating spores powder weight are 0.02~0.1g/g culture mediums, and every gram of conidia powder contains 108A spore
Son, 30~40 DEG C, 12~36h of fermentation time of fermentation temperature add the water of pig blood weight 1/3, and stirring 1 time, secondary temperature elevation, until 40
~45 DEG C, 24~48h is kept, obtains swine blood fermentation;
(2) digest
The swine blood fermentation of step (1) is taken, is crushed, adds in the water of swine blood fermentation identical weight, crosses colloid mill, homogenate makes
PH to 6.0~7.0 is adjusted with 1mol/L sodium hydroxide solutions or 1mol/L hydrochloric acid solutions, addition 0.1~1% is (with swine blood fermentation
Weight meter) papain and flavor protease, papain:The mass ratio of flavor protease is 2:1,1~3h is kept,
90~100 DEG C are warming up to, keeps 5~15min to get swine blood fermentation enzymolysis liquid.
Pet feeding experiment 1:
By pet phagostimulant made from the method for the present invention, sprayed in cat food in the ratio of cat food total weight 2.5%, band
After storing 15d, 10 Garfields is selected to carry out pet feeding experiment, experiment number of days is 4 days, phagostimulating effect is observed, with commercially available cat
Grain compares, and adds the particulate material referred to as experiment cat food 1 of pet phagostimulant made from the method for the present invention.
(1) preferred test
Experimental method:Two bowls for experiment cat are provided simultaneously, the experiment cat food of equivalent and commercially available is respectively put into bowl
Cat food observes and records the first selection of every experiment cat.(bowl position is exchanged every other day)
(2) double basin method tests
Experimental method:Two bowls for experiment cat are provided simultaneously, the experiment cat food of equivalent and commercially available is respectively put into bowl
Cat food weighs and records primary feeding process (9:00-15:00) consumption of two kinds of food in.And consumption rate is calculated as follows
(CR).(bowl position is exchanged every other day)
1 cat food Preference test result of table
Sample | Test cat food 1 | Commercially available cat food |
Preferred cat quantity (only) | 30 | 10 |
Consumption rate CR (%) | 64.17 | 35.83 |
It is tested by first choice test and double basin methods, the results are shown in Table 1, finds experiment cat food 1, that is, is added to present invention side
The palatability of the cat food of pet phagostimulant will be substantially better than commercially available cat food made from method.
Pet feeding experiment 2:
By pet phagostimulant made from the method for the present invention, sprayed in cat food in the ratio of cat food total weight 4.5%, band
After storing 15d, 10 Garfields is selected to carry out pet feeding experiment, experiment number of days is 4 days, phagostimulating effect is observed, with commercially available cat
Grain compares, and adds the particulate material referred to as experiment cat food 2 of pet phagostimulant made from the method for the present invention.
(1) preferred test
Experimental method:Two bowls for experiment cat are provided simultaneously, the experiment cat food 2 of equivalent and commercially available is respectively put into bowl
Cat food observes and records the first selection of every experiment cat.(bowl position is exchanged every other day)
(2) double basin method tests
Experimental method:Two bowls for experiment cat are provided simultaneously, the experiment cat food 2 of equivalent and commercially available is respectively put into bowl
Cat food weighs and records primary feeding process (9:00-15:00) consumption of two kinds of food in.And consumption rate is calculated as follows
(CR).(bowl position is exchanged every other day)
2 cat food Preference test result of table
Sample | Test cat food 2 | Commercially available cat food |
Preferred cat quantity (only) | 29 | 11 |
Consumption rate CR (%) | 55.52 | 44.48 |
It is tested by first choice test and double basin methods, the results are shown in Table 2, finds experiment cat food 2, that is, is added to present invention side
The palatability of the cat food of pet phagostimulant will be substantially better than commercially available cat food made from method.
Thermal-reactive pet phagostimulant of the present invention sprays heat when in use, in the ratio of pet food total weight 1%~5%
Response type pet phagostimulant.
Claims (5)
- A kind of 1. method that thermal-reactive pet phagostimulant is prepared using swine blood fermentation, it is characterised in that:Step is as follows:Take the swine blood fermentation enzymolysis liquid of raw material gross mass 20-80%, the reduced sugar of raw material gross mass 0.5-6%, raw material gross mass The amino acid of 0.5-6%, the yeast extract of raw material gross mass 2-5%, after mixing plus water complements to 100%, carries out thermal response, Temperature is 100-110 DEG C, and reaction time 1-3h is subsequently cooled to room temperature, you can obtains thermal-reactive pet phagostimulant.
- 2. the method according to claim 1 that thermal-reactive pet phagostimulant is prepared using swine blood fermentation, it is characterised in that: The reduced sugar is glucose, two or more mixture in xylose, ribose.
- 3. the method according to claim 1 that thermal-reactive pet phagostimulant is prepared using swine blood fermentation, it is characterised in that: The amino acid is glycine, two kinds in alanine, l-methionine, aspartic acid, arginine, L-cysteine hydrochloride Or two or more mixture.
- 4. the method according to claim 1 that thermal-reactive pet phagostimulant is prepared using swine blood fermentation, it is characterised in that: The thermal-reactive pet phagostimulant sprays thermal-reactive pet when in use, in the ratio of pet food total weight 1%~5% Phagostimulant.
- 5. the method according to any one of claims 1 to 4 that thermal-reactive pet phagostimulant is prepared using swine blood fermentation, It is characterized in that:The preparation method of the swine blood fermentation enzymolysis liquid is as follows:(1) fermentPig blood with wheat bran, corn flour is mixed, is stirred evenly, as culture medium, the wherein dosage of pig blood is the 40% of gross mass ~80%, the mass ratio of wheat bran and corn flour is 1:2, it sterilizes, temperature is 121 DEG C, and time 20min is cooled to room temperature, Inoculated aspergillus niger, aspergillus niger inoculating spores powder weight are 0.02~0.1g/g culture mediums, and every gram of conidia powder contains 108A spore, hair 30~40 DEG C, 12~36h of fermentation time of ferment temperature, adds the water of pig blood weight 1/3, and stirring 1 time, secondary temperature elevation, until 40~45 DEG C, 24~48h is kept, obtains swine blood fermentation;(2) digestThe swine blood fermentation of step (1) is taken, is crushed, adds in the water of swine blood fermentation identical weight, crosses colloid mill, is homogenized, is used 1mol/L sodium hydroxide solutions or 1mol/L hydrochloric acid solutions adjust pH to 6.0~7.0, and addition 0.1~1% is (with swine blood fermentation weight Gauge) papain and flavor protease, papain:The mass ratio of flavor protease is 2:1,1~3h is kept, is risen Temperature keeps 5~15min to get swine blood fermentation enzymolysis liquid to 90~100 DEG C.
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CN110651888A (en) * | 2019-04-12 | 2020-01-07 | 株式会社三多 | Method for producing fermented product of pig blood, and antibacterial composition and chicken feed composition using the fermented product |
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CN101331936A (en) * | 2008-07-31 | 2008-12-31 | 天津春发食品配料有限公司 | Method for preparing meat essence by fermentation |
CN103099181A (en) * | 2013-03-01 | 2013-05-15 | 贵州大学 | Preparation method of natural thermal reaction meat flavor base material |
CN103960635A (en) * | 2014-05-29 | 2014-08-06 | 天津春宇食品配料有限公司 | Method for preparing meat-flavor essence |
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2017
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Publication number | Priority date | Publication date | Assignee | Title |
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CN101331936A (en) * | 2008-07-31 | 2008-12-31 | 天津春发食品配料有限公司 | Method for preparing meat essence by fermentation |
CN103099181A (en) * | 2013-03-01 | 2013-05-15 | 贵州大学 | Preparation method of natural thermal reaction meat flavor base material |
CN103960635A (en) * | 2014-05-29 | 2014-08-06 | 天津春宇食品配料有限公司 | Method for preparing meat-flavor essence |
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Cited By (1)
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CN110651888A (en) * | 2019-04-12 | 2020-01-07 | 株式会社三多 | Method for producing fermented product of pig blood, and antibacterial composition and chicken feed composition using the fermented product |
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