CN108168979A - A kind of freezing microtome for Botany Experiment - Google Patents

A kind of freezing microtome for Botany Experiment Download PDF

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Publication number
CN108168979A
CN108168979A CN201810126558.2A CN201810126558A CN108168979A CN 108168979 A CN108168979 A CN 108168979A CN 201810126558 A CN201810126558 A CN 201810126558A CN 108168979 A CN108168979 A CN 108168979A
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CN
China
Prior art keywords
blade
needle
blade support
support plate
blades
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201810126558.2A
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Chinese (zh)
Inventor
张育栋
张宇洋
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xinchang County Qixing Street Xinwei Machinery Factory
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Xinchang County Qixing Street Xinwei Machinery Factory
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xinchang County Qixing Street Xinwei Machinery Factory filed Critical Xinchang County Qixing Street Xinwei Machinery Factory
Priority to CN201810126558.2A priority Critical patent/CN108168979A/en
Publication of CN108168979A publication Critical patent/CN108168979A/en
Withdrawn legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873Cutting or cleaving

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The present invention provides a kind of freezing microtome for Botany Experiment, including No.1 blade, chooses needle and No. two blades;The top fixed symmetrical of the clamping fixture seat is provided with totally two No.1 electric pushrods, and the top of No.1 electric pushrod is fixedly installed that there are one lower fixtures.The setting of No.1 blade and No. two blades, be conducive to the convenience used, No. three electric pushrods of control push blade support plate to be moved to clamping fixture seat direction during use, No.1 blade and No. two blades can then contacted with plant tuber, simultaneously plant tuber can directly be cut out a piece of and be clipped among No.1 blade and No. two blades, choosing the side of needle at this time can also be formed directly into this plant section, then the promotion of No. two electric pushrods is controlled, which to choose needle rotation and provoke the plant section for choosing needle side upwards, can remove slice, only need once-through operation that can successfully be sliced plant during slice, and two blades can play the role of Anti-curling simultaneously in slicing processes, effectively reduce operation difficulty.

Description

A kind of freezing microtome for Botany Experiment
Technical field
The invention belongs to technical field of experiment equipment more particularly to a kind of freezing microtomes for Botany Experiment.
Background technology
Frozen section is tissue to be made to freeze to reach a kind of method that certain hardness is sliced rapidly using low temperature, because of it It is not required to by dehydration and transparent and etc., there is quick, simplicity, easy to operate, and tissue can be preserved better Enzymatic activity more fully preserves the immunocompetence of tissue antigen, is that in situ hybridization, enzyme histochemistry and immunohistochemistry etc. are real Common method in testing.The type of frozen section is more, and common slicer is broadly divided into two major class:Open freezing microtome With constant temperature freezing microtome.
Such as application is 201120394516.0 patent, discloses a kind of automatic exhibition piece thermostat slicer freezer, including Box body, freezing moist closet, control panel, knife rest combination unit, blade, slice head and head control wheel are sliced, it is described The end side of knife rest combination unit is equipped with the ventilation opening parallel with blade upper surface, while the upper table of ventilation opening and knife rest combination unit Face paste is closed;The end side of ventilation opening is attracted deposit chlorination equipment.The slicer is not only simple in structure, and slice can open up piece automatically, can It is smooth ultra-thin not have to anti-roll plate, while waste paper being capable of automatic suction.
Again for example application No. is 201610070466.8 patent, disclose a kind of slicing and slitting machine, existing cut to solve Existing for machine can only by fruit and the processing of axis block into strips the problem of, the present invention takes motor to be mounted on rack (1) On, motor is connect by belt with rotating shaft transmission, and two shafts are separately positioned on the both ends in rack (1), at shaft both ends Sprocket wheel (2) is installed, the sprocket wheel (2) at rack (1) both ends is connected by chain (4), is provided on chain (4) by predetermined distance Slicer (3) and slitting knife (8) are equipped with guide pressing device (5) above chain (4), and guide pressing device (5) passes through branch Frame is connect with rack (1).Fruit and axis block can be processed into strips, and fruit and axis block can processed so as to have The advantages of slabbing.
It is found based on above-mentioned retrieval, two patents make in combination with most freezing microtomes of the prior art Used time has the following problems:For existing freezing microtome when being sliced to the plant after freezing, being first will be after freezing Plant is fixed, and plant then is cut a plane, then plant is set, plant is cut one using blade after with blade Piece and by section developing, is then placed on glass slide and is tested, just needed using blade operation in slice so twice again, Operation is more time-consuming and laborious, and it also requires dedicated tool prevents slice from crimping, improves operation difficulty.
Then, in view of this, improvement is studied for existing structure and missing, is provided a kind of for Botany Experiment Freezing microtome, to achieve the purpose that with more more practical value.
Invention content
In order to solve the above technical problem, the present invention provides a kind of freezing microtome for Botany Experiment, to solve Existing freezing microtome is first to fix the plant after freezing, then uses knife when being sliced to the plant after freezing Plant is cut a plane by piece, then plant set after distance using blade plant is cut it is a piece of and by section developing, so It being placed on glass slide and is tested again afterwards, just needed using blade operation in slice twice in this way, operation is more time-consuming and laborious, And it also requires dedicated tool prevents from being sliced the problem of crimping, improving operation difficulty.
The present invention is reached for the purpose and effect of the freezing microtome of Botany Experiment by technological means in detail below Into:
A kind of freezing microtome for Botany Experiment, including pedestal, blade support plate, No.1 blade support, one Number blade adjustment bolt, No.1 electric pushrod, clamping fixture seat, upper fixture fixed link, lower fixture, upper fixture, emulsion layer, No.1 knife Piece chooses needle, No. two electric pushrods, chooses needle supporting rod, No. three electric pushrods, No. three electric pushrod fixed seats, No. two blade supports Bar and No. two blades;There are one blade support plates for the top movable installation of the pedestal;The top side of the blade support plate There are one No.1 blade support and No. two blade supports for fixed symmetrical setting;The side of No. two blade supports is fixed There are one No. two blades for setting;There are one No.1 blade adjustment spiral shells for the inside top insertion installation of the No.1 blade support Bolt, and there are one No.1 blades for the side fixed setting of No.1 blade adjustment bolt;The top opposite side of the blade support plate It is fixedly installed one and chooses needle supporting rod;The top movable for choosing needle supporting rod is equipped with one and chooses needle;The blade support It is also set up at the top of plate there are one No. two electric pushrods, and the top of No. two electric pushrods is flexibly connected with choosing the bottom of needle;Institute The top side fixed setting of pedestal is stated there are one No. three electric pushrod fixed seats;The side of No. three electric pushrod fixed seats Fixed symmetrical is equipped with two No. three electric pushrods, and side of the opposite side of No. three electric pushrods with blade support plate is fixed Connection;There are one clamping fixture seats for the top opposite side fixed setting of the pedestal;The top fixed symmetrical of the clamping fixture seat is provided with Totally two No.1 electric pushrods, and the top of No.1 electric pushrod is fixedly installed there are one lower fixture;The top of the clamping fixture seat Also by two upper fixture fixed link fixed symmetrical installations, there are two upper fixtures in portion;The inside of the lower fixture and upper fixture it is interior Side is fixedly installed one layer of emulsion layer.
Further, the No.1 blade and No. two blades are circular configuration.
Further, the No.1 blade is can left-right movable type structure.
Further, the needle of choosing is rotary type structure.
Further, the bottom of No. two electric pushrods is connect by shaft with the top movable of blade support plate, and two The top of number electric pushrod is flexibly connected by shaft with choosing the bottom of needle.
Further, the needle of choosing is in normal state with the center of circle of No.1 blade and the center of circle of upper fixture in same level On face.
Further, the bottom fixed symmetrical of the blade support plate is provided with two inverted T font bulge-structures, and this two The bottom and both sides of a protrusion are movably set with several idler wheels, and the inside top of pedestal is additionally provided with corresponding groove-like knot Structure.
Further, there are one empty groove-like structures corresponding with clamping fixture seat size for the side setting of the blade support plate.
Compared with prior art, the present invention has the advantages that:
The setting of No.1 blade and No. two blades, is conducive to the convenience used, and No. three electric pushrods of control push away during use Cutting blade support plate is moved to clamping fixture seat direction, No.1 blade and No. two blades can then contacted with plant tuber, simultaneously Plant tuber can directly be cut out a piece of and be clipped among No.1 blade and No. two blades, choosing the side of needle at this time also can directly wear Enter in this plant section, No. two electric pushrod promotions is then controlled to choose needle rotation and provoke the plant section for choosing needle side upwards Slice can be removed, when slice only needs once-through operation that can successfully be sliced plant, and two blades can in slicing processes Play the role of Anti-curling simultaneously, effectively reduce operation difficulty.
Description of the drawings
Fig. 1 is the side structure schematic view of the present invention.
Structure diagram when Fig. 2 is the slice of the present invention.
Fig. 3 is the overlooking the structure diagram of the present invention.
Fig. 4 is the main cross section structure diagram of the present invention.
Fig. 5 is Fig. 1 partial enlargement structural representations of the present invention.
Fig. 6 is also Fig. 1 partial enlargement structural representations of the present invention.
Fig. 7 is Fig. 4 partial enlargement structural representations of the present invention.
Fig. 8 is the blade support plate overlooking the structure diagram of the present invention.
In figure:Pedestal -1;Blade support plate -2;No.1 blade support -3;No.1 blade adjustment bolt -4;No.1 is electronic Push rod -5;Clamping fixture seat -6;Upper fixture fixed link -7;Lower fixture -8;Upper fixture -9;Emulsion layer -10;No.1 blade -11;Choose needle- 12;No. two electric pushrods -13;Choose needle supporting rod -14;No. three electric pushrods -15;No. three electric pushrod fixed seats -16;No. two knives Piece supporting rod -17;No. two blades -18.
Specific embodiment
The present invention is described further below in conjunction with attached drawing:
Embodiment:
As shown in attached drawing 1 to attached drawing 8:
The present invention provides a kind of freezing microtome for Botany Experiment, including pedestal 1, blade support plate 2, No.1 knife Piece supporting rod 3, No.1 blade adjustment bolt 4, No.1 electric pushrod 5, clamping fixture seat 6, upper fixture fixed link 7, lower fixture 8, upper folder Tool 9, No.1 blade 11, chooses 12, No. two electric pushrods 13 of needle, chooses 14, No. three electric pushrods 15, three of needle supporting rod emulsion layer 10 Number 16, No. two blade supports 17 of electric pushrod fixed seat and No. two blades 18;
There are one blade support plates 2 for the top movable installation of pedestal 1;The top side fixed symmetrical of blade support plate 2 is set No.1 blade support 3 and No. two blade supports 17 there are one putting;The side of No. two blade supports 17 is fixedly installed one A No. two blades 18;The inside top of No.1 blade support 3 is embedded in No.1 blade adjustment bolt 4 there are one installations, and No.1 There are one No.1 blades 11 for the side fixed setting of blade adjustment bolt 4;
The top opposite side of blade support plate 2 is fixedly installed one and chooses needle supporting rod 14;Choose the top of needle supporting rod 14 It is movably installed with one and chooses needle 12;The top of blade support plate 2 is also set up there are one No. two electric pushrods 13, and No. two electronic push away The top of bar 13 is flexibly connected with choosing the bottom of needle 12;The top side fixed setting of pedestal 1 is consolidated there are one No. three electric pushrods Reservation 16;The side fixed symmetrical of No. three electric pushrod fixed seats 16 is equipped with two No. three electric pushrods 15, and No. three electronic Side of the opposite side of push rod 15 with blade support plate 2 is fixedly connected;
There are one clamping fixture seats 6 for the top opposite side fixed setting of pedestal 1;The top fixed symmetrical of clamping fixture seat 6 is provided with altogether Two No.1 electric pushrods 5, and the top of No.1 electric pushrod 5 is fixedly installed there are one lower fixture 8;The top of clamping fixture seat 6 Also by two 7 fixed symmetrical of upper fixture fixed link installations, there are two upper fixtures 9;The inside of lower fixture 8 and the inside of upper fixture 9 It is fixedly installed one layer of emulsion layer 10.
Wherein, the No.1 blade 11 and No. two blades 18 are circular configuration, are carrying out left and right adjustment No.1 blade 11 Position when No.1 blade 11 can constantly rotate, edge of a knife direction always can be towards the fixed bit of plant tuber after rotation in this way It puts, meets cutting use demand.
Wherein, the No.1 blade 11 for can left-right movable type structure, rotation No.1 blade adjustment bolt 4 during use No.1 blade 11 is driven to move left and right, then can adjust the spacing between No.1 blade 11 and No. two blades 18 as needed, Spacing is the thickness of slice cut out, and two blades can play the role of Anti-curling simultaneously in slicing processes, is effectively dropped Low operation difficulty.
Wherein, the needle 12 of choosing is rotary type structure, No. three electric pushrods 15 is controlled to push using controller during slice Blade support plate 2 is moved to 6 direction of clamping fixture seat, and No.1 blade 11 and No. two blades 18 can then contacted with plant tuber, Simultaneously plant tuber can directly be cut out a piece of and be clipped among No.1 blade 11 and No. two blades 18, choose the side of needle 12 at this time Also it can be formed directly into this plant section, No. two promotions of electric pushrod 13 is then controlled to choose needle 12 and rotates and will choose 12 side of needle Plant section is provoked upwards, and slide then can be used to pick up slice and observe, easy to operate.
Wherein, the bottom of No. two electric pushrods 13 is connect by shaft with the top movable of blade support plate 2, No. two The top of electric pushrod 13 is flexibly connected by shaft with choosing the bottom of needle 12, and choosing needle 12 when No. two promotions of electric pushrod 13 rotates When, choosing needle 12 and the junction position at No. two 13 tops of electric pushrod can also change, therefore can make No. two electric pushrods 13 Direction generate inclination, the two shafts can facilitate No. two electric pushrods 13 to be rotated.
Wherein, the needle 12 of choosing is in normal state with the center of circle of No.1 blade 11 and the center of circle of upper fixture 9 in same water In plane, plant tuber can directly be cut out a piece of and is clipped in No.1 blade 11 and No. two blades 18 by two blades during use Between, because choosing needle 12 can also move and choose needle 12 and the center of plant tuber is in same plane simultaneously, needle is chosen at this time 12 side can be also formed directly into this plant section, and taking-up is facilitated to be sliced.
Wherein, the bottom fixed symmetrical of the blade support plate 2 is provided with two inverted T font bulge-structures, and the two The bottom and both sides of protrusion are movably set with several idler wheels, and the inside top of pedestal 1 is additionally provided with corresponding groove-like knot Structure, the protrusion of 2 bottom of blade support plate when moving of blade support plate 2 can be stuck in the inside of pedestal 1, and protrusion outside this A little idler wheels can effectively reduce resistance when blade support plate 2 moves, and T fonts protrusion can carry in the interior Slideslip of pedestal 1 The stability that high blade support plate 2 moves.
Wherein, empty groove-like structure corresponding with 6 size of clamping fixture seat there are one the side settings of the blade support plate 2, The side of clamping fixture seat 6 can be embedded into the groove of 2 side of blade support plate when blade support plate 2 moves, and avoid blade support plate 2 It is collided with clamping fixture seat 6 and cutting is impacted.
The specifically used mode of the present embodiment and effect:
In the present invention, when use, first connects the power supply of peripheral control unit, and the connection mode of controller is existing mature technology, It does not do to tire out in detail herein and state, then the plant tuber freezed is placed on to the inside top of two lower fixtures 8, use outside Controller controls two No.1 electric pushrods 5 that two lower fixtures 8 is pushed to move up, and makes lower fixture 8 and upper fixture 9 by plant block Stem firm grip, and the outer wall of plant tuber is caused because the setting of emulsion layer 10 can play the role of anti-skidding be avoided that again Damage, then rotating No.1 blade adjustment bolt 4 can drive No.1 blade 11 to move left and right, and then can adjust as needed Spacing between No.1 blade 11 and No. two blades 18, spacing are the thickness of slice cut out, and control is used after the completion of adjustment Device controls No. three electric pushrods 15 that blade support plate 2 is pushed to be moved to 6 direction of clamping fixture seat, then can make No.1 blade 11 and two Number blade 18 is contacted with plant tuber, while can directly be cut out plant tuber a piece of and be clipped in No.1 blade 11 and No. two blades Among 18, choosing the side of needle 12 at this time can also be formed directly into this plant section, then No. two electric pushrods 13 be controlled to push It chooses needle 12 and rotates and provoke the plant section for choosing 12 side of needle upwards, slide then can be used to pick up slice and observes, Only need once-through operation that can successfully be sliced plant during slice, and two blades can play Anti-curling simultaneously in slicing processes Effect, effectively reduces operation difficulty.
Using technical solution of the present invention or those skilled in the art under the inspiration of technical solution of the present invention, if Similar technical solution is counted out, and reaches above-mentioned technique effect, is to fall into protection scope of the present invention.

Claims (8)

1. a kind of freezing microtome for Botany Experiment, it is characterised in that:This is used for the freezing microtome of Botany Experiment Including pedestal, blade support plate, No.1 blade support, No.1 blade adjustment bolt, No.1 electric pushrod, clamping fixture seat, upper folder Tool fixed link, lower fixture, upper fixture, emulsion layer, No.1 blade, choose needle, No. two electric pushrods, choose needle supporting rod, No. three it is electronic Push rod, No. three electric pushrod fixed seats, No. two blade supports and No. two blades;There are one the top movable installations of the pedestal Blade support plate;There are one No.1 blade support and No. two blades for the top side fixed symmetrical setting of the blade support plate Supporting rod;There are one No. two blades for the side fixed setting of No. two blade supports;The top of the No.1 blade support Embedded installation is there are one No.1 blade adjustment bolt on the inside of portion, and there are one one for the side fixed setting of No.1 blade adjustment bolt Number blade;The top opposite side of the blade support plate is fixedly installed one and chooses needle supporting rod;The top for choosing needle supporting rod Portion is movably installed with one and chooses needle;It is also set up at the top of the blade support plate there are one No. two electric pushrods, and No. two electronic The top of push rod is flexibly connected with choosing the bottom of needle;The top side fixed setting of the pedestal is consolidated there are one No. three electric pushrods Reservation;The side fixed symmetrical of No. three electric pushrod fixed seats is equipped with two No. three electric pushrods, and No. three electronic push away Side of the opposite side of bar with blade support plate is fixedly connected;There are one fixtures for the top opposite side fixed setting of the pedestal Seat;The top fixed symmetrical of the clamping fixture seat is provided with totally two No.1 electric pushrods, and the top of No.1 electric pushrod is solid There are one lower fixtures for fixed setting;Also by two upper fixture fixed link fixed symmetrical installations, there are two upper at the top of the clamping fixture seat Fixture;The inside of the lower fixture and the inside of upper fixture are fixedly installed one layer of emulsion layer.
2. it to be used for the freezing microtome of Botany Experiment as described in claim 1, it is characterised in that:The No.1 blade and No. two Blade is circular configuration.
3. it to be used for the freezing microtome of Botany Experiment as claimed in claim 1 or 2, it is characterised in that:The No.1 blade is It can left-right movable type structure.
4. it to be used for the freezing microtome of Botany Experiment as described in claim 1, it is characterised in that:The needle of choosing is rotary type Structure.
5. the freezing microtome of Botany Experiment to be used for as described in claim 1 or 4, it is characterised in that:Described No. two electronic to push away The bottom of bar is connect by shaft with the top movable of blade support plate, and needle by shaft and is chosen in the top of No. two electric pushrods Bottom is flexibly connected.
6. the freezing microtome of Botany Experiment to be used for as described in claims 1 or 2 or 3 or 4 or 5, it is characterised in that:It is described to choose Needle is in normal state with the center of circle of No.1 blade and the center of circle of upper fixture in same level.
7. the freezing microtome of Botany Experiment to be used for as described in claim 1 or 5, it is characterised in that:The blade support plate Bottom fixed symmetrical be provided with two inverted T font bulge-structures, and bottoms of the two protrusions and both sides are movably set with Several idler wheels, and the inside top of pedestal is additionally provided with corresponding groove-like structure.
8. the freezing microtome of Botany Experiment to be used for as described in claim 1 or 5 or 7, it is characterised in that:The blade support There are one empty groove-like structures corresponding with clamping fixture seat size for the side setting of plate.
CN201810126558.2A 2018-02-08 2018-02-08 A kind of freezing microtome for Botany Experiment Withdrawn CN108168979A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810126558.2A CN108168979A (en) 2018-02-08 2018-02-08 A kind of freezing microtome for Botany Experiment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810126558.2A CN108168979A (en) 2018-02-08 2018-02-08 A kind of freezing microtome for Botany Experiment

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109049069A (en) * 2018-08-12 2018-12-21 江苏捷通管业科技有限公司 A kind of power pipe Cutting platform
CN111413342A (en) * 2020-04-20 2020-07-14 华中科技大学 Microscopic imaging system for frozen section
CN114112598A (en) * 2021-12-07 2022-03-01 贵州大学 A frozen slicer for phytology experiment

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109049069A (en) * 2018-08-12 2018-12-21 江苏捷通管业科技有限公司 A kind of power pipe Cutting platform
CN111413342A (en) * 2020-04-20 2020-07-14 华中科技大学 Microscopic imaging system for frozen section
CN111413342B (en) * 2020-04-20 2021-03-26 华中科技大学 Microscopic imaging system for frozen section
CN114112598A (en) * 2021-12-07 2022-03-01 贵州大学 A frozen slicer for phytology experiment
CN114112598B (en) * 2021-12-07 2023-10-13 贵州大学 A frozen section machine for botanic experiment

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Application publication date: 20180615

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