CN108142627A - The antioxidative method and device of Pu'er tea is improved using high-pressure pulse electric - Google Patents
The antioxidative method and device of Pu'er tea is improved using high-pressure pulse electric Download PDFInfo
- Publication number
- CN108142627A CN108142627A CN201711475954.8A CN201711475954A CN108142627A CN 108142627 A CN108142627 A CN 108142627A CN 201711475954 A CN201711475954 A CN 201711475954A CN 108142627 A CN108142627 A CN 108142627A
- Authority
- CN
- China
- Prior art keywords
- tea
- voltage
- pulse electric
- hpef
- pressure pulse
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/36—Reducing or removing alkaloid content; Preparations produced thereby; Extracts or infusions thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- General Preparation And Processing Of Foods (AREA)
- Tea And Coffee (AREA)
Abstract
The antioxidative method and device of Pu'er tea is improved using high-pressure pulse electric the present invention relates to a kind of, belongs to Pu'er tea processing technique field, the antioxidative method of Pu'er tea is improved using high-pressure pulse electric, the specific steps are：1）Solar dried green tea is processed into Pu'er cooked tea by national standard；2）By step 1）Ripe tea, which is placed in high-pressure pulse electric, to be handled.The present invention provides a kind of new physical method to improve Pu'er tea polysaccharide anti-oxidative activity, its polyphenol content can be reduced simultaneously, and a large amount of tea polyphenols is promoted to be converted into the theabrownin that can improve Pu'er cooked tea antioxidation, accelerate its ageing, it is made to be aged effect consistent with ageing naturally.And this method is the extraction and exploitation of natural in Pu'er tea, Pu'er tea deep processing, exploitation Pu'er tea functional food technical support is provided.
Description
Technical field
The invention belongs to Pu'er tea processing technique field, specifically, being related to a kind of general using high-pressure pulse electric raising
The antioxidative method and device of Pu'er tea tea.
Background technology
Geographical sign product of the Pu'er tea as Yunnan Province, because it with distinguishing character and many health-care efficacies receives state
Inside and outside love tea people's likes.And the health-care efficacy of Pu'er tea has：Solution fat, foster the spirit of nobility, prolong life, lowering blood-fat and reducing weight, prevention and treatment of coronary heart disease,
Antihypertensive, aid digestion, diuresis, relieves the effect of alcohol etc. at anti-aging.Yunnan puer tea it is existing so magical the effect of, be because
Itself contain abundant bioactive ingredients.Because of it, to have antifatigue, anti-bacteria and anti-virus, aid digestion etc. many again for these substances
Effect and largely extracted, and then various drugs and health products are made, for the health-care efficacy of Pu'er tea, most important effect
Mechanism is exactly：Its antioxidation.And in recent years, the antioxidation of Pu'er tea has progressed into Pu'er tea industry research person
The visual field, how to improve its antioxidant activity and also become one of research hotspot of tea field.
Invention content
To improve the inoxidizability of Pu'er tea, the present invention handles Pu'er tea by using high-pressure pulse electric, to reduce tea
In polyphenol content, improve tea in content of tea polysaccharide, so as to improve the antioxidant activity of Pu'er tea.
The invention is realized by the following technical scheme, and specific implementation step is as follows：
The antioxidative method of Pu'er tea is improved using high-pressure pulse electric, the specific steps are：
1) after solar dried green tea being processed into Pu'er cooked tea by national standard；
2) the ripe tea of step 1) is placed in high-pressure pulse electric and handled.
In step 2), the voltage of the high-pressure pulse electric is 12-22KV, frequency 80-162HZ, and action time is
55min。
The antioxidative device of Pu'er tea, which is improved, using high-pressure pulse electric includes high-voltage pulse power source 1, electrode plate I 2, electricity
Pole plate II 3, treatment box 4, the electrode plate I 2, electrode plate II 3 are connected on respectively on 1 cathode of high-voltage pulse power source and anode, and
Electrode plate I 2, electrode plate II 3 are installed in parallel in treatment box 4, and vacuum extractor 5 is provided on treatment box 4.
The high-voltage pulse power source 1 includes pressure regulator 5, step-up transformer 6, rectification circuit 7, main capacitance 8, current-limiting resistance
9th, discharge capacity 10, high-voltage switch gear 11, controller 13, regulating resistor 14, the output terminal and step-up transformer of the pressure regulator 5
6 input terminal connection, the output terminal of step-up transformer 6 are connect with 7 input terminal of rectification circuit, and main capacitance 8, discharge capacity 10 are distinguished
It is connected in parallel on the output terminal of rectification circuit 7, current-limiting resistance 9 is in series between main capacitance 8, discharge capacity 10, discharge capacity 10
High-voltage switch gear 11, regulating resistor 14, the control signal of high-voltage switch gear 11 and the output terminal of controller 13 are connected on output terminal
Connection.
Beneficial effects of the present invention：
The present invention provides a kind of new physical method to improve Pu'er tea polysaccharide anti-oxidative activity, can reduce its tea
Polyphenol content promotes a large amount of tea polyphenols to be converted into the theabrownin that can improve Pu'er cooked tea antioxidation, accelerates its ageing, and
It is consistent with ageing naturally that it is aged effect.And this method is the extraction of natural and exploitation, Pu'er tea in Pu'er tea
Deep processing, exploitation Pu'er tea functional food provide technical support.
Description of the drawings
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is the structure diagram of high-voltage pulse power source.
Fig. 3 tea polyphenols are to the clearance rate of OH and voltage, the three-dimensional relationship figure of frequency；
Fig. 4 tea polyphenols are to O2Clearance rate and voltage, frequency three-dimensional relationship figure；
Fig. 5 tea polyphenols are to the removing of DPPH free radicals and voltage, the three-dimensional relationship figure of frequency；
The total reducing power of Fig. 6 tea polyphenols and voltage, the three-dimensional relationship figure of frequency；
Fig. 7 tea polysaccharides are to the clearance rate of OH and voltage, the three-dimensional relationship figure of frequency；
Fig. 8 tea polysaccharides are to O2Clearance rate and voltage, frequency three-dimensional relationship figure
Fig. 9 tea polysaccharides are to the removing of DPPH free radicals and voltage, the three-dimensional relationship figure of frequency；
The total reducing power of Figure 10 tea polysaccharides and voltage, the three-dimensional relationship figure of frequency；
The absorbance value standard curve of Figure 11 tea polyphenols；
The absorbance value standard curve of Figure 12 tea polysaccharides.
In figure, 1- high-voltage pulse power sources, 2- electrode plates I, 3- electrode plates II, 4- treatment boxes, 5- pressure regulators, 6- boosting transformations
Device, 7- rectification circuits, 8- main capacitances, 9- current-limiting resistances, 10- discharge capacities, 11- high-voltage switch gears, 13- controllers, 14- pressure regulation electricity
Resistance.
Specific embodiment
Below in conjunction with the embodiment of the present invention and attached drawing, the technical solution in the embodiment of the present invention is carried out clear, complete
Ground describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.Based on this
Embodiment in invention, the every other reality that those of ordinary skill in the art are obtained without making creative work
Example is applied, shall fall within the protection scope of the present invention.
The antioxidative method of Pu'er tea is improved using high-pressure pulse electric, the specific steps are：
1) after solar dried green tea being processed into Pu'er cooked tea by national standard；
2) the ripe tea of step 1) is placed in high-pressure pulse electric and handled.
In step 2), the voltage of the high-pressure pulse electric is 12-22KV, frequency 80-162HZ, and action time is
55min。
The antioxidative device of Pu'er tea, which is improved, using high-pressure pulse electric includes high-voltage pulse power source 1, electrode plate I 2, electricity
Pole plate II 3, treatment box 4, the electrode plate I 2, electrode plate II 3 are connected on respectively on 1 cathode of high-voltage pulse power source and anode, and
Electrode plate I 2, electrode plate II 3 are installed in parallel in treatment box 4, and vacuum extractor 5 is provided on treatment box 4.
The high-voltage pulse power source 1 includes pressure regulator 5, step-up transformer 6, rectification circuit 7, main capacitance 8, current-limiting resistance
9th, discharge capacity 10, high-voltage switch gear 11, controller 13, regulating resistor 14, the output terminal and step-up transformer of the pressure regulator 5
6 input terminal connection, the output terminal of step-up transformer 6 are connect with 7 input terminal of rectification circuit, and main capacitance 8, discharge capacity 10 are distinguished
It is connected in parallel on the output terminal of rectification circuit 7, current-limiting resistance 9 is in series between main capacitance 8, discharge capacity 10, discharge capacity 10
High-voltage switch gear 11, regulating resistor 14, the control signal of high-voltage switch gear 11 and the output terminal of controller 13 are connected on output terminal
Connection.
Embodiment 1
The three-level Pu'er cooked tea for originating in Lincang Prefecture by 2011 be placed in electrode plate I 2 in treatment box 4, electrode plate II 3 it
Between, the 220V power supplys of high-voltage pulse power source 1 are connected, 220V alternating currents exchange high electric by pressure regulator 5 with the generation of step-up transformer 6
Pressure, after rectification circuit 7, charges to its main capacitance 8, and pass through current-limiting resistance 9 and charge to discharge capacity 10, so as to
DC high voltage is generated, controller 13 controls high-voltage switch gear 11 to be connected, when high-voltage switch gear 11 is connected under trigger signal control；It is logical
It is 13kV to cross regulating resistor 14 and adjust output voltage, and exporting trigger signal by controller 13 controls cut-offfing frequently for high-voltage switch gear 11
Rate is 80Hz so as to adjust pulse frequency；Then the high pressure at 10 its both ends of discharge capacity is discharged rapidly by medium, forms high-tension pulse
Punching, high-voltage pulse are handled by II 3 pairs of electrode plate I 2, electrode plate Pu'er cooked teas.
Embodiment 2
The three-level Pu'er cooked tea for originating in Lincang Prefecture by 2011 be placed in electrode plate I 2 in treatment box 4, electrode plate II 3 it
Between, the 220V power supplys of high-voltage pulse power source 1 are connected, 220V alternating currents exchange high electric by pressure regulator 5 with the generation of step-up transformer 6
Pressure, after rectification circuit 7, charges to its main capacitance 8, and pass through current-limiting resistance 9 and charge to discharge capacity 10, so as to
DC high voltage is generated, controller 13 controls high-voltage switch gear 11 to be connected, when high-voltage switch gear 11 is connected under trigger signal control；It is logical
It is 18kV to cross regulating resistor 14 and adjust output voltage, and exporting trigger signal by controller 13 controls cut-offfing frequently for high-voltage switch gear 11
Rate is 99Hz so as to adjust pulse frequency；Then the high pressure at 10 its both ends of discharge capacity is discharged rapidly by medium, forms high-tension pulse
Punching, high-voltage pulse are handled by II 3 pairs of electrode plate I 2, electrode plate Pu'er cooked teas.
Embodiment 3
The three-level Pu'er cooked tea for originating in Lincang Prefecture by 2011 be placed in electrode plate I 2 in treatment box 4, electrode plate II 3 it
Between, the 220V power supplys of high-voltage pulse power source 1 are connected, 220V alternating currents exchange high electric by pressure regulator 5 with the generation of step-up transformer 6
Pressure, after rectification circuit 7, charges to its main capacitance 8, and pass through current-limiting resistance 9 and charge to discharge capacity 10, so as to
DC high voltage is generated, controller 13 controls high-voltage switch gear 11 to be connected, when high-voltage switch gear 11 is connected under trigger signal control；It is logical
It is 22kV to cross regulating resistor 14 and adjust output voltage, and exporting trigger signal by controller 13 controls cut-offfing frequently for high-voltage switch gear 11
Rate is 99Hz so as to adjust pulse frequency；Then the high pressure at 10 its both ends of discharge capacity is discharged rapidly by medium, forms high-tension pulse
Punching, high-voltage pulse are handled by II 3 pairs of electrode plate I 2, electrode plate Pu'er cooked teas.
Experimental analysis
The parameter for influencing high-pressure pulse electric (HPEF) is mainly voltage, frequency and action time.According to project team early period
Achievement in research, setting action time position 55min, frequency 80Hz, 99Hz, 121Hz, 139Hz, 162Hz, voltage 12kV-
22kV, totally 11 levels, parameter are as shown in table 1.
The setting (Time=55min) of 1 HPEF voltages of table, frequency
Four indexs are included for the measure of tea polyphenols, tea polysaccharide antioxidant activity：
OH Scavenging activities, O2Scavenging activity, the free Scavenging activity of DPPH free radicals, total reducing power.
(1) measure of OH abilities is removed：
Hydroxyl radical free radical clearance rate (%)=[1- ((A1-A2))/A0]×100
(2) O is removed2The measure of ability：
Superoxide anion clearance rate (%)=[1- ((A1-A2))/A0]×100
(3) measure of scavenging ability of DPPH free radical：
DPPH free radical scavenging activities (%)=[1- ((A1-A2))/A0]×100
(4) measure of the total reducing power of tea polysaccharide：Measure its total reducing power.
Absorbance value A is measured at λ=360nm.Reflect total reducing power indirectly with A, the value the big, illustrates that it is total
Reducing power is stronger, on the contrary then weaker.
The absorbance measurement of tea polyphenols：
Tea polyphenols standard solution 1.0mL, 2.0mL, 3.0mL, 4.0mL is drawn respectively in 4 50mL volumetric flasks, and respectively
Distilled water is added to be settled to 10mL, then adds in 10mL ferrous tartrate solution, is eventually adding phosphate buffer (pH=7.5) to quarter
It spends, cuvette is packed into after mixing, using blank reagent as reference, its absorbance value (A) is measured at λ=540nm, draws bid
Directrix curve, standard curve are as shown in figure 11.
The absorbance measurement of tea polysaccharide：
Alcohol deposition method：According to the difference of solution boiling point, moisture a large amount of in millet paste is removed using Rotary Evaporators, is added in
Sevage solution (chloroforms：N-butanol=4：1 mixed solution), 20~30min is vibrated, takes supernatant liquid, then to
1 times of absolute ethyl alcohol is wherein added in, stands for 24 hours filtering afterwards, drying can obtain tea polysaccharide crude extract.
Then the measure of content of tea polysaccharide draws absorbance standard curve, such as using Phenol-Sulfuric Acid Spectrophotometry
Shown in Figure 12.
1st, influences of the HPEF to polyphenol content in tea sample
According to polyphenol calibration curve equation y=7.5350x+0.0123 (R2=0.9997) blank group, is calculated and through difference
In each group tea sample of HPEF processing in crude extract tea polyphenols content, content is shown in Table 2.
Polyphenol content in 2 tea polyphenols crude extract of table
(1) HPEF removes tea polyphenols the influence of OH abilities
Tea polyphenols in the tea sample after HPEF is handled carry out gradually the clearance rate of OH using mathematical software (Matlab)
Regression analysis, using binary cubic equation to the voltage (X of its Scavenging activity (Y) and HPEF1), frequency (X2) relationship carry out it is more
First nonlinear fitting, establishes equation, obtains optimal fitting equation.
Y=-107.31+34.214X1+0.21157X2-0.00413X1X2-2.0427X1 2-0.000597X2 2+
0.040711X1 3
Its P value of optimum regression model is 2.56e-08 (being less than 0.001), this model is notable, and intercept and each system in model
Several P values are equal<0.05, then the model can be used to predict the Scavenging activity of OH tea polyphenols in the tea sample after HPEF is handled
Analysis.According to modeling rendering three-dimensional relationship figure, as shown in Figure 3.It can be obtained from Fig. 3, when HPEF voltages are 12-18kV, tea is more
Phenol first enhances, then reduce the Scavenging activity of OH with the increase of its frequency；When HPEF voltages are more than 18kV, clearance rate is whole
On increase with the increase of frequency.When HPEF conditions are near 22kV, tea polyphenols are best to the scavenging effect of OH.
(2) HPEF removes O to tea polyphenols2·–The influence of ability
Using Matlab to tea polyphenols in the tea sample after HPEF is handled to O2·–Clearance rate carry out stepwise regression analysis, make
With binary cubic equation to the voltage (X of its Scavenging activity (Y) and HPEF1), frequency (X2) relationship carry out nonlinear multivariable plan
It closes, establishes equation, obtain optimal fitting equation.
Y=1703.9-169.71X1-30.035X2+3.1584X1X2+4.3041X1 2+0.12554X2 2-0.08333X1 2X2-
0.01357X1X2 2+0.000367X1 2X2 2
Its P value of optimum regression model is 1.26e-07 (being less than 0.001), this model is notable, and intercept and each system in model
Several P values are equal<0.05, then can be used the model to tea polyphenols in the tea sample after HPEF is handled to O2-Scavenging activity carry out it is pre-
Survey analysis.According to modeling rendering three-dimensional relationship figure, as shown in Figure 4.By Tu Ke get, when HPEF frequencies are less than 100Hz, remove
Effect reduces with becoming larger for voltage.Near low pressure low frequency, high-voltage pulse electric field energy improves tea polyphenols in Pu'er tea and removes
O2-ability.When HPEF frequencies be less than 100Hz, voltage be less than 16kV when, HPEF can improve tea polyphenols to O2-it is external
Scavenging activity, scavenging effect are most strong.
(3) influences of the HPEF to tea polyphenols scavenging ability of DPPH free radical
Successive Regression is carried out to the clearance rate of DPPH free radicals to tea polyphenols in the tea sample after HPEF is handled using Matlab
Analysis carries out its Scavenging activity (Y) with the voltage (X1) of HPEF, the relationship of frequency (X2) using binary cubic equation polynary non-
Linear fit establishes equation, obtains optimal fitting equation.
Y=838.81-6.0972X1-19.195X2+0.18985X1 2+0.16874X2 2-0.000478X2 3
Its P value of optimum regression model is 6.52e-12 (being less than 0.001), this model is notable, and intercept and each system in model
Several P values are equal<0.05, then can be used the model to tea polyphenols in the tea sample after HPEF is handled to the Scavenging activities of DPPH free radicals into
Row forecast analysis.According to modeling rendering three-dimensional relationship figure, as shown in Figure 5.
From fig. 5, it can be seen that in one timing of HPEF voltages, tea polyphenols are to the scavenging effect of DPPH free radicals with its frequency
Increase first reduces, rear to increase, then reduces；When one timing of HPEF frequencies, clearance rate changes fluctuation less with its voltage, then influences
Tea polyphenols are to frequency that the principal element of the Scavenging activity of DPPH free radicals is HPEF.When HPEF frequencies are near 140Hz, tea
Polyphenol is best to the elimination effect of DPPH free radicals.
(4) influence of HPEF total reducing powers to tea polyphenols
Stepwise regression analysis is carried out to the total reducing power of tea polyphenols in the tea sample after HPEF is handled using Matlab, uses two
First cubic equation is to the voltage (X of its total reducing power (Y) and HPEF1), frequency (X2) relationship carry out Multiple non-linear,
Equation is established, obtains optimal fitting equation.
Y=10.558+0.23452X1-0.2919X2+0.000518X1X2-0.00849X1 2+0.002314X2 2-
0.00000611X1 3
Its P value of optimum regression model is 2.43e-12 (being less than 0.001), this model is notable, and intercept and each system in model
Several P values are equal<0.05, then the model can be used to carry out forecast analysis to the total reducing power of tea polyphenols in the tea sample after HPEF is handled.
According to modeling rendering three-dimensional relationship figure, as shown in Figure 6.
It will be appreciated from fig. 6 that as wavelength X=360nm, one timing of HPEF frequencies, the total reducing power of tea polyphenols with its voltage increasing
Add first to increase and reduce afterwards；When one timing of HPEF voltages, total reducing power first reduces with becoming larger for its frequency, rear to increase, then subtracts
It is small.When voltage is 16-20kV, when frequency is near 80Hz or 160Hz, HPEF can improve total reducing power of tea polyphenols.
Analysis of conclusion
Pu'er tea is handled using high-pressure pulse electric and extracts the tea polyphenols in tea sample.Comparative analysis each group tea sample
The content of middle tea polyphenols is it can be found that appropriate HPEF can reduce polyphenol content, wherein the tea of the 22nd group (22kV/99Hz) is more
Phenol content is minimum, is 0.0067mg/mL, reduces 43.22% than blank group polyphenol content, a large amount of tea polyphenols is promoted to be changed into
The substances such as the theabrownin of its antioxidant activity can be improved, promote its ageing, and it is consistent with ageing naturally to be aged effect.It utilizes
To tea polyphenols, each scavenging effect and total reducing power carry out stepwise regression analysis, and establish corresponding optimum regression mould to Matlab
Type draws corresponding three-dimensional relationship figure.The result shows that the significance test probability P of optimum regression model<0.01, i.e. model is shown
It writes.1) when voltage is 22kV, tea polyphenols are best to the scavenging effect of OH；2) when frequency is less than 100Hz, voltage is less than 16kV
When, tea polyphenols to O2-Scavenging activity it is maximum；3) principal element for influencing Scavenging activity is frequency；When frequency is attached in 140Hz
When near, tea polyphenols are maximum to the scavenging effect of DPPH free radicals；4) voltage is 16kV-20kV, and frequency is near 80Hz or 160Hz
When, total reducing power of tea polyphenols is most strong.
To sum up result of study shows that suitable high-voltage pulse electric field energy reduces Pu'er tea polyphenol content and improves its antioxygen
Change activity.Treated that polyphenol content is minimum for the high-pressure pulse electric of 22kV/99Hz, is 0.0067mg/mL, than blank group tea
Polyphenol content reduces 43.22%.It can be seen that HPEF is acted on Pu'er cooked tea, its polyphenol content can be reduced, is promoted
A large amount of tea polyphenols are converted into the theabrownin that can improve Pu'er cooked tea antioxidation, accelerate its ageing, and its be aged effect with
Naturally ageing is consistent.
2nd, influences of the HPEF to content of tea polysaccharide in tea sample
According to polysaccharide calibration curve equation y=8.2200x+0.0147 (R2=0.9997) blank group, is calculated and through difference
In each group tea sample of HPEF processing in crude extract tea polysaccharide content, content is shown in Table 3.
Content of tea polysaccharide in 3 tea polysaccharide crude extract of table
(1) HPEF removes tea polysaccharide the influence of OH abilities
Stepwise regression analysis is carried out to the clearance rate of OH to tea polysaccharide in the tea sample after HPEF is handled using Matlab, is made
With binary cubic equation to the voltage (X of its Scavenging activity (Y) and HPEF1), frequency (X2) relationship carry out nonlinear multivariable plan
It closes, establishes equation, obtain optimal fitting equation.
Y=94.232+0.55945X1+0.061745X2-0.035053X1 2-0.00049171X2 2+0.00027475X1X2+
0.00067568X1 3+0.000001202X2 3
Its P value of optimum regression model is 5.34e-08 (being less than 0.001), this model is notable, and intercept and each system in model
Several P values are equal<0.05, then the model can be used to predict the Scavenging activity of OH tea polysaccharide in the tea sample after HPEF is handled
Analysis.According to modeling rendering three-dimensional relationship figure, as shown with 7.
It can be obtained from Fig. 7, when HPEF voltages are 12-18kV, tea polysaccharide is to the Scavenging activity of OH with the increasing of its frequency
Adding first enhances, then reduces；When HPEF voltages are more than 18kV, clearance rate increases on the whole with the increase of frequency.When HPEF items
When part is near 22kV/162Hz, tea polysaccharide is best to the scavenging effect of OH.
(2) HPEF removes O to tea polysaccharide2The influence of ability
Using Matlab to tea polysaccharide in the tea sample after HPEF is handled to O2Clearance rate carry out stepwise regression analysis,
Using binary cubic equation to the voltage (X of its Scavenging activity (Y) and HPEF1), frequency (X2) relationship carry out nonlinear multivariable plan
It closes, establishes equation, obtain optimal fitting equation.
Y=-35.784+2.2099X1+2.1266X2-0.0073939X2 2-0.028182X1X2
Its P value of optimum regression model is 3.75e-08 (being less than 0.001), this model is notable, and intercept and each system in model
Several P values are equal<0.05, then it can be used the model to tea polysaccharide in the tea sample after HPEF is handled to O2Scavenging activity carry out it is pre-
Survey analysis.According to modeling rendering three-dimensional relationship figure, as shown in Figure 8.
Can be obtained by Fig. 8, in set voltage range (12-22kV), tea polysaccharide to O2-scavenging effect with frequency
Becoming larger for rate first enhances, then reduce；When HPEF frequencies are less than 100Hz, change the size of its voltage its clearance rate substantially without
Significant change；When HPEF frequencies are more than 100Hz, clearance rate is in reduction trend with the increase of voltage.When HPEF voltages are less than
14kV, when frequency is 120-140Hz, tea polysaccharide is to O2Scavenging effect it is best.
(3) influences of the HPEF to tea polysaccharide scavenging ability of DPPH free radical
Successive Regression is carried out to the clearance rate of DPPH free radicals to tea polysaccharide in the tea sample after HPEF is handled using Matlab
Analysis, using binary cubic equation to the voltage (X of its Scavenging activity (Y) and HPEF1), frequency (X2) relationship carry out it is polynary non-
Linear fit establishes equation, obtains optimal fitting equation.
Y=109.72-0.083284X1-0.173017X2+0.00059166X2 2
Its P value of optimum regression model is 4.54e-08 (being less than 0.001), this model is notable, and intercept and each system in model
Several P values are equal<0.05, then can be used the model to tea polysaccharide in the tea sample after HPEF is handled to the Scavenging activities of DPPH free radicals into
Row forecast analysis.According to modeling rendering three-dimensional relationship figure, as shown in Figure 9.
From fig. 9, it can be seen that in the timing of HPEF voltages one, by tea polysaccharide to the clearance rates of DPPH free radicals on the whole with
The increase of its frequency and reduce；When one timing of HPEF frequencies, clearance rate fluctuates little with the increase of voltage.Therefore, tea is influenced
The principal element that polysaccharide removes the ability of DPPH free radicals is HPEF frequencies, and when HPEF voltages are less than 14kV, frequency is in 80Hz
When neighbouring, HPEF can enhance the ability that tea polysaccharide removes DPPH free radicals, and scavenging effect is best at this time.
(4) influence of HPEF total reducing powers to tea polysaccharide
Stepwise regression analysis is carried out to the total reducing power of tea polysaccharide in the tea sample after HPEF is handled using Matlab, uses two
First cubic equation is to the voltage (X of its total reducing power (Y) and HPEF1), frequency (X2) relationship carry out Multiple non-linear,
Equation is established, obtains optimal fitting equation.
Y=-44.954+5.3492X1+0.70703X2-0.14596X1 20-0.0026079X2 2-0.081965X1X2+
0.0022262X1 2X2+0.00029876X1X2 2-0.000008071X1 2X2 2
Its P value of optimum regression model is 9.22e-08 (being less than 0.001), this model is notable, and intercept and each system in model
Several P values are equal<0.05, then the model can be used to carry out forecast analysis to the total reducing power of tea polysaccharide in the tea sample after HPEF is handled.
According to modeling rendering three-dimensional relationship figure, as shown in Figure 10.
As shown in Figure 10, as wavelength X=360nm, when HPEF voltages are more than 14kV, total reducing power with its frequency increasing
It is in reducing tendency on the whole greatly；When HPEF voltages be less than 14kV when, total reducing power first enhances with the increase of its frequency, after subtract
It is weak.When HPEF voltages are more than 18kV, when frequency is near 80Hz, total reducing power of tea polysaccharide is most strong.
Analysis of conclusion
Pu'er tea is handled using high-pressure pulse electric and extracts the tea polysaccharide in tea sample.Comparative analysis each group tea sample
The content of middle tea polysaccharide can improve the recovery rate of its tea polysaccharide, i.e., it can be found that handled using appropriate HPEF tea sample
Content of tea polysaccharide increases, wherein the content of tea polysaccharide of the 2nd group (13kV/80Hz) is up to 0.1672mg/mL, than blank group tea sample tea
Polyoses content is higher by 5.09%.Therefore, content of tea polysaccharide in tea sample can be improved by appropriate HPEF processing.Utilize Matlab pairs
Each scavenging effect and total reducing power carry out stepwise regression analysis, and establish corresponding optimum regression model, draw corresponding three
Tie up relational graph.The result shows that the significance test probability P of optimum regression model<0.01, i.e. model is notable.1) to the clear of OH
Except effect：When near 22kV/162Hz, the ability that tea polysaccharide removes OH is most strong；2) to O2-scavenging effect：Work as HPEF
Voltage be less than 14kV, frequency be 120-140Hz when, tea polysaccharide to O2-scavenging effect it is best；3) to the clear of DPPH free radicals
Except effect：The principal element for influencing tea polysaccharide scavenging ability of DPPH free radical is frequency.When voltage is less than 14kV, frequency is in 80Hz
When neighbouring, the ability that tea polysaccharide removes DPPH free radicals is most strong.4) total reducing power：When HPEF voltages are more than 18kV, frequency exists
When near 80Hz, total reducing power is most strong.
To sum up result of study, most of the content of tea polysaccharide is higher than sky in tea polysaccharide crude extract in tea sample after HPEF is handled
White group.Treated that content of tea polysaccharide is up to 0.1672mg/mL for the high-pressure pulse electric of 13kV/80Hz, higher than blank group content
Go out 5.09%.It can be seen that being handled using appropriate HPEF tea sample, tea polysaccharide in tea polysaccharide crude extract can be improved
Content.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
It crosses above preferred embodiment the present invention is described in detail, however, those skilled in the art should understand that, can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (4)
1. improve the antioxidative method of Pu'er tea using high-pressure pulse electric, it is characterised in that：The specific steps are：
1）Solar dried green tea is processed as Pu'er cooked tea by national standard；
2）By step 1）Ripe tea, which is placed in high-pressure pulse electric, to be handled.
2. according to claim 1 improve the antioxidative method of Pu'er tea using high-pressure pulse electric, it is characterised in that：
Step 2）In, the voltage of the high-pressure pulse electric is 12-22KV, frequency 80-162HZ, action time 55min.
3. improve the antioxidative device of Pu'er tea using high-pressure pulse electric, it is characterised in that：Described utilizes high-voltage pulse
Electric field improves the antioxidative device of Pu'er tea and includes high-voltage pulse power source（1）, electrode plate I（2）, electrode plate II（3）, treatment box
（4）, the electrode plate I（2）, electrode plate II（3）It is connected on high-voltage pulse power source respectively（1）On cathode and anode, and electrode plate I
（2）, electrode plate II（3）It is installed in parallel in treatment box（4）It is interior, treatment box（4）On be provided with vacuum extractor（5）.
4. according to claim 3 improve the antioxidative device of Pu'er tea using high-pressure pulse electric, it is characterised in that：
The high-voltage pulse power source（1）Including pressure regulator（5）, step-up transformer（6）, rectification circuit（7）, main capacitance（8）, current limliting electricity
Resistance（9）, discharge capacity（10）, high-voltage switch gear（11）, controller（13）, regulating resistor（14）, the pressure regulator（5）Output
End and step-up transformer（6）Input terminal connection, step-up transformer（6）Output terminal and rectification circuit（7）Input terminal connects, main
Capacitance（8）, discharge capacity（10）It is connected in parallel on rectification circuit respectively（7）Output terminal on, main capacitance（8）, discharge capacity（10）It
Between be in series with current-limiting resistance（9）, discharge capacity（10）Output terminal on be connected with high-voltage switch gear（11）, regulating resistor（14）, it is high
It compresses switch（11）Control signal and controller（13）Output terminal connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711475954.8A CN108142627A (en) | 2017-12-29 | 2017-12-29 | The antioxidative method and device of Pu'er tea is improved using high-pressure pulse electric |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711475954.8A CN108142627A (en) | 2017-12-29 | 2017-12-29 | The antioxidative method and device of Pu'er tea is improved using high-pressure pulse electric |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108142627A true CN108142627A (en) | 2018-06-12 |
Family
ID=62463878
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711475954.8A Pending CN108142627A (en) | 2017-12-29 | 2017-12-29 | The antioxidative method and device of Pu'er tea is improved using high-pressure pulse electric |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108142627A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110353067A (en) * | 2019-08-27 | 2019-10-22 | 温州科技职业学院 | A kind of production method of blue or green money willow instant powder |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102488031A (en) * | 2011-12-12 | 2012-06-13 | 云南农业大学 | Method for reducing content of tea polyphenol in Pu'er tea finished product |
-
2017
- 2017-12-29 CN CN201711475954.8A patent/CN108142627A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102488031A (en) * | 2011-12-12 | 2012-06-13 | 云南农业大学 | Method for reducing content of tea polyphenol in Pu'er tea finished product |
Non-Patent Citations (2)
| Title |
|---|
| 方明群: "《铁路机车车辆运用、检修、维护、保养与标准规范全书》", 30 September 2003, 吉林电子出版社 * |
| 王白娟，等: "高压脉冲电场对普洱茶中茶多酚含量的影响", 《西南农业学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110353067A (en) * | 2019-08-27 | 2019-10-22 | 温州科技职业学院 | A kind of production method of blue or green money willow instant powder |
| CN110353067B (en) * | 2019-08-27 | 2022-02-01 | 温州科技职业学院 | Preparation method of instant cyclocarya paliurus powder |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101773593B (en) | Method for preparing antioxidative active extractive of sweet potato leaves | |
| CN112608393B (en) | Method for extracting stropharia rugoso-annulata polysaccharide | |
| CN103013669B (en) | Preparation method of tobacco leaf moisturizing liquor for lowering tar content and benzopyrene content | |
| CN107822948A (en) | With after-sun restorative, anti-oxidant, white-skinned face function composition and preparation method thereof | |
| CN108142627A (en) | The antioxidative method and device of Pu'er tea is improved using high-pressure pulse electric | |
| CN103251658B (en) | Antrodia Camphorata extraction concentrate and manufacture method thereof | |
| CN110669588A (en) | Distillation method for extracting ginger essential oil and application of distillation method in cosmetics | |
| CN102816637A (en) | Combined extraction method of Paeonia ostii seed oil and Paeonia ostii seed total flavonoids | |
| CN106491473A (en) | A kind of alimentary water with crease-resistant effect | |
| Jiang et al. | Effects of extraction conditions on crude polysaccharides and antioxidant activities of the lion's mane medicinal mushroom, Hericium erinaceus (Agaricomycetes) | |
| CN101152255A (en) | Method of preparing aqueous extract concrete of ficus microcarpa leaf | |
| CN112168873A (en) | Response surface method optimized extraction process of total flavonoids of Asparagus racemosus | |
| CN104490667B (en) | A kind of cosmetics containing the anti-skin ageing of loquat seed extract | |
| CN106674186A (en) | Method and reagent for extracting anthocyanin | |
| CN108690145B (en) | Microwave-assisted extraction method of hawthorn polysaccharide | |
| CN115948490A (en) | Method for improving activity of polygonatum polysaccharide extracting solution | |
| CN105919140B (en) | A kind of preparation method of pinenut dietary fiber | |
| CN105175561B (en) | A kind of preparation method of anti-oxidant straw mushroom polysaccharide | |
| CN110721210A (en) | Extraction process and application of acanthopanax bark flavone | |
| CN105831549A (en) | Soybean dreg extract natural composite anti-oxidant and preparation method thereof | |
| CN103289968B (en) | From the water-soluble extracting liquid of sweet potato, be separated and prepare the method for polyphenoloxidase, beta-amylase and storage protein | |
| CN102367283B (en) | Laminarin as well as extraction method and application thereof | |
| CN101982183A (en) | Preparation method of Astilbe chinensis root extract and antioxidant application of Astilbe chinensis root extract | |
| CN104479045A (en) | Preparation method for Sinkiang turnip polysaccharide having immunoregulatory activity | |
| CN104163873A (en) | Technology for extracting polysaccharide from roots of pleurotus geesteranus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180612 |
|
| RJ01 | Rejection of invention patent application after publication |