CN108130093A - A kind of glyphosate microbial degradation preparation and preparation method thereof - Google Patents
A kind of glyphosate microbial degradation preparation and preparation method thereof Download PDFInfo
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- CN108130093A CN108130093A CN201711419842.0A CN201711419842A CN108130093A CN 108130093 A CN108130093 A CN 108130093A CN 201711419842 A CN201711419842 A CN 201711419842A CN 108130093 A CN108130093 A CN 108130093A
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- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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Abstract
Disclosed by the invention to belong to microorganisms technical field, specially a kind of glyphosate microbial degradation preparation and preparation method thereof, the glyphosate microbial degradation preparation includes following constituent：Organic fertilizer：30 50 parts；Microorganism mixed liquor：70 80 parts；Chitin：10 15 parts and 40 50 parts of plant ash, the preparation method of the glyphosate microbial degradation preparation include the following steps：S1：Prepare microorganism mixed liquor；S2：Mixing：Organic fertilizer, chitin and plant ash are mixed with microorganism mixed liquor, it stirs evenly, stir speed (S.S.) is 50 60 revs/min, is stirred 20 30 minutes, you can, a kind of glyphosate microbial degradation preparation that the invention proposes and preparation method thereof, it is grown using glyphosate as unique phosphorus source, carbon source and nitrogen source, can be by glyphosate degradation into sarcosine and AMPA, it and can be degradable by glyphosate in a short time, it is environmentally safe, and the invention can also realize the recovery and improvement to soil.
Description
Technical field
The present invention relates to microorganisms technical field, specially a kind of glyphosate microbial degradation preparation and preparation method thereof.
Background technology
Glyphosate is the effective active chemical composition in widely used many herbicides, it is received in the use of European Union
Strict supervision.Glyphosate has no direct relation with genetically modified crops, and non-transgenic crop will equally beat herbicide glyphosate.Grass
Sweet phosphine is increasingly strict to the requirement of its residual quantity both at home and abroad as a kind of great pesticide of usage amount.For agricultural and sideline product medium-height grass
The control of sweet phosphine residual quantity, if reducing its residue of glyphosate using chemically or physically being handled, not only
Treating capacity is big, of high cost, but also has secondary pollution or nutritive loss.For this purpose, it is proposed that a kind of glyphosate microorganism drops
Solve preparation and preparation method thereof.
Invention content
The purpose of the present invention is to provide a kind of glyphosate microbial degradation preparation and preparation method thereof, to solve the above-mentioned back of the body
The problem of being proposed in scape technology.
To achieve the above object, the present invention provides following technical solution：A kind of glyphosate microbial degradation preparation, the grass are sweet
Phosphine microbial degradation preparation includes following constituent：
Organic fertilizer：30-50 parts；Microorganism mixed liquor：70-80 parts；Chitin：10-15 parts and 40-50 parts of plant ash.
Preferably, the organic fertilizer is animal wastes.
Preferably, the microorganism mixed liquor includes anthropi, arthrobacterium, Flavobacterium, rhizobium and penicillium chrysogenum element
Bacterium.
Preferably, the component ratio between the anthropi, arthrobacterium, Flavobacterium, rhizobium and Chrysogenum
For：2:1:1:3:2.
A kind of preparation method of glyphosate microbial degradation preparation, the preparation method packet of the glyphosate microbial degradation preparation
Include following steps：
S1：Prepare microorganism mixed liquor：Anthropi, arthrobacterium, Flavobacterium, rhizobium and Chrysogenum are inoculated with
Into the culture dish equipped with culture solution, glyphosate is then added in, the addition of glyphosate is 100 mg/litres, stir culture 4-5
My god, stir speed (S.S.) is 100-130 revs/min, and whipping temp is 28-32 degrees Celsius, then improves the concentration of glyphosate, domestication training
It supports 5-6 days, the last culture solution of domestication successively is taken to carry out 10-12 times of gradient dilution, culture solution is transferred in fermentation tank and is fermented,
Fermentation temperature is 32-34 degrees Celsius, and stir speed (S.S.) is 130-150 revs/min, ferments 1-2 days, obtains microorganism mixed liquor；
S2：Mixing：Organic fertilizer, chitin and plant ash with microorganism mixed liquor are mixed, stirred evenly, stir speed (S.S.) is
It 50-60 revs/min, stirs 20-30 minutes, you can.
Preferably, in the step S1, improve glyphosate concentration when, successively according to 200 mg/litres, 400 milligrams/
It rises, 600 mg/litres and 800 mg/litres improve culture solution glyphosate concentration.
Compared with prior art, the beneficial effects of the invention are as follows：A kind of glyphosate microbial degradation system that the invention proposes
Agent and preparation method thereof is grown using glyphosate as unique phosphorus source, carbon source and nitrogen source, can by glyphosate degradation into
Sarcosine and AMPA, and can be degradable by glyphosate in a short time, it is environmentally safe, and the invention can also be realized pair
The recovery and improvement of soil.
Specific embodiment
The technical solution in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example is only part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's all other embodiments obtained without making creative work belong to the model that the present invention protects
It encloses.
The present invention provides a kind of glyphosate microbial degradation preparation, which includes following composition
Ingredient：
Organic fertilizer：30-50 parts, the organic fertilizer is animal wastes；Microorganism mixed liquor：70-80 parts, the microorganism mixes
It closes liquid and includes anthropi, arthrobacterium, Flavobacterium, rhizobium and Chrysogenum, the anthropi, arthrobacterium, yellow bar
Component ratio between bacterium, rhizobium and Chrysogenum is：2:1:1:3:2；Chitin：10-15 parts and plant ash 40-50
Part.
The invention also provides a kind of preparation method of glyphosate microbial degradation preparation：
Embodiment 1
The preparation method of the glyphosate microbial degradation preparation includes the following steps：
S1：Prepare microorganism mixed liquor：Anthropi, arthrobacterium, Flavobacterium, rhizobium and Chrysogenum are inoculated with
Into the culture dish equipped with culture solution, glyphosate is then added in, the addition of glyphosate is 100 mg/litres, stir culture 4.5
My god, stir speed (S.S.) is 115 revs/min, and whipping temp is 30 degrees Celsius, then improves the concentration of glyphosate, domestication culture 5.5
My god, the last culture solution of domestication successively is taken to carry out 11 times of gradient dilutions, culture solution is transferred in fermentation tank and is fermented, fermentation temperature
It it is 33 degrees Celsius, stir speed (S.S.) is 140 revs/min, ferments 1.5 days, obtains microorganism mixed liquor, in the concentration for improving glyphosate
When, culture solution glyphosate to be improved according to 200 mg/litres, 400 mg/litres, 600 mg/litres and 800 mg/litres dense successively
Degree；
S2：Mixing：Organic fertilizer, chitin and plant ash with microorganism mixed liquor are mixed, stirred evenly, stir speed (S.S.) is
It 55 revs/min, stirs 25 minutes, you can.
In the present embodiment, organic fertilizer：40 parts, organic fertilizer is animal wastes；Microorganism mixed liquor：75 parts, microorganism mixes
It closes liquid and includes anthropi, arthrobacterium, Flavobacterium, rhizobium and Chrysogenum, anthropi, arthrobacterium, Flavobacterium, root
Component ratio between knurl bacterium and Chrysogenum is：2:1:1:3:2；Chitin：12.5 parts and 45 parts of plant ash.
Embodiment 2
The preparation method of the glyphosate microbial degradation preparation includes the following steps：
S1：Prepare microorganism mixed liquor：Anthropi, arthrobacterium, Flavobacterium, rhizobium and Chrysogenum are inoculated with
Into the culture dish equipped with culture solution, glyphosate is then added in, the addition of glyphosate is 100 mg/litres, stir culture 5 days,
Stir speed (S.S.) is 130 revs/min, and whipping temp is 32 degrees Celsius, then improves the concentration of glyphosate, and domestication culture 6 days takes most
Domestication culture solution carries out 12 times of gradient dilutions successively afterwards, and culture solution is transferred in fermentation tank and is fermented, and fermentation temperature is taken the photograph for 34
Family name's degree, stir speed (S.S.) are 150 revs/min, ferment 2 days, obtain microorganism mixed liquor, in the concentration for improving glyphosate, successively
Culture solution glyphosate concentration is improved according to 200 mg/litres, 400 mg/litres, 600 mg/litres and 800 mg/litres；
S2：Mixing：Organic fertilizer, chitin and plant ash with microorganism mixed liquor are mixed, stirred evenly, stir speed (S.S.) is
It 60 revs/min, stirs 30 minutes, you can.
In the present embodiment, organic fertilizer：50 parts, organic fertilizer is animal wastes；Microorganism mixed liquor：80 parts, microorganism mixes
It closes liquid and includes anthropi, arthrobacterium, Flavobacterium, rhizobium and Chrysogenum, anthropi, arthrobacterium, Flavobacterium, root
Component ratio between knurl bacterium and Chrysogenum is：2:1:1:3:2；Chitin：11 parts and 50 parts of plant ash.
Embodiment 3
The preparation method of the glyphosate microbial degradation preparation includes the following steps：
S1：Prepare microorganism mixed liquor：Anthropi, arthrobacterium, Flavobacterium, rhizobium and Chrysogenum are inoculated with
Into the culture dish equipped with culture solution, glyphosate is then added in, the addition of glyphosate is 100 mg/litres, stir culture 4 days,
Stir speed (S.S.) is 100 revs/min, and whipping temp is 28 degrees Celsius, then improves the concentration of glyphosate, and domestication culture 5 days takes most
Domestication culture solution carries out 10 times of gradient dilutions successively afterwards, and culture solution is transferred in fermentation tank and is fermented, and fermentation temperature is taken the photograph for 32
Family name's degree, stir speed (S.S.) are 130 revs/min, ferment 1 day, obtain microorganism mixed liquor, in the concentration for improving glyphosate, successively
Culture solution glyphosate concentration is improved according to 200 mg/litres, 400 mg/litres, 600 mg/litres and 800 mg/litres；
S2：Mixing：Organic fertilizer, chitin and plant ash with microorganism mixed liquor are mixed, stirred evenly, stir speed (S.S.) is
It 50 revs/min, stirs 20 minutes, you can.
In the present embodiment, organic fertilizer：36 parts, organic fertilizer is animal wastes；Microorganism mixed liquor：78 parts, microorganism mixes
It closes liquid and includes anthropi, arthrobacterium, Flavobacterium, rhizobium and Chrysogenum, anthropi, arthrobacterium, Flavobacterium, root
Component ratio between knurl bacterium and Chrysogenum is：2:1:1:3:2；Chitin：15 parts and 41 parts of plant ash.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of glyphosate microbial degradation preparation, it is characterised in that：The glyphosate microbial degradation preparation includes following composition
Ingredient：
Organic fertilizer：30-50 parts；Microorganism mixed liquor：70-80 parts；Chitin：10-15 parts and 40-50 parts of plant ash.
2. a kind of glyphosate microbial degradation preparation according to claim 1, it is characterised in that：The organic fertilizer is animal
Excrement.
3. a kind of glyphosate microbial degradation preparation according to claim 1, it is characterised in that：The microorganism mixed liquor
Including anthropi, arthrobacterium, Flavobacterium, rhizobium and Chrysogenum.
4. a kind of glyphosate microbial degradation preparation according to claim 1, it is characterised in that：The anthropi, section
Component ratio between bacillus, Flavobacterium, rhizobium and Chrysogenum is：2:1:1:3:2.
5. a kind of preparation method of glyphosate microbial degradation preparation, it is characterised in that：The glyphosate microbial degradation preparation
Preparation method includes the following steps：
S1：Prepare microorganism mixed liquor：Anthropi, arthrobacterium, Flavobacterium, rhizobium and Chrysogenum are seeded to dress
Have in the culture dish of culture solution, then add in glyphosate, the addition of glyphosate is 100 mg/litres, and stir culture 4-5 days is stirred
It is 100-130 revs/min to mix rate, and whipping temp is 28-32 degrees Celsius, then improves the concentration of glyphosate, domestication culture 5-6
My god, the last culture solution of domestication successively is taken to carry out 10-12 times of gradient dilution, culture solution is transferred in fermentation tank and is fermented, is fermented
Temperature is 32-34 degrees Celsius, and stir speed (S.S.) is 130-150 revs/min, ferments 1-2 days, obtains microorganism mixed liquor；
S2：Mixing：Organic fertilizer, chitin and plant ash with microorganism mixed liquor are mixed, stirred evenly, stir speed (S.S.) 50-
It 60 revs/min, stirs 20-30 minutes, you can.
6. a kind of preparation method of glyphosate microbial degradation preparation according to claim 5, it is characterised in that：The step
In rapid S1, in the concentration for improving glyphosate, successively according to 200 mg/litres, 400 mg/litres, 600 mg/litres and 800 millis
G/l improve culture solution glyphosate concentration.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711419842.0A CN108130093A (en) | 2017-12-25 | 2017-12-25 | A kind of glyphosate microbial degradation preparation and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711419842.0A CN108130093A (en) | 2017-12-25 | 2017-12-25 | A kind of glyphosate microbial degradation preparation and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728386A (en) * | 2018-06-20 | 2018-11-02 | 荆门市拓达科技有限公司 | A kind of preparation method of glyphosate solid-state degradation bacterial agent |
| CN111825272A (en) * | 2020-05-25 | 2020-10-27 | 安徽普偌森环保科技有限公司 | Microbial degradation treatment process for rural domestic sewage |
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| CN103011416A (en) * | 2012-10-24 | 2013-04-03 | 刘颖超 | Application of bacillus cereus in degrading glyphosate |
| CN106478291A (en) * | 2016-09-18 | 2017-03-08 | 广西大学 | A kind of soil conditioner being applied to glyphosate contaminated soil |
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2017
- 2017-12-25 CN CN201711419842.0A patent/CN108130093A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103011416A (en) * | 2012-10-24 | 2013-04-03 | 刘颖超 | Application of bacillus cereus in degrading glyphosate |
| CN106478291A (en) * | 2016-09-18 | 2017-03-08 | 广西大学 | A kind of soil conditioner being applied to glyphosate contaminated soil |
Non-Patent Citations (2)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728386A (en) * | 2018-06-20 | 2018-11-02 | 荆门市拓达科技有限公司 | A kind of preparation method of glyphosate solid-state degradation bacterial agent |
| CN111825272A (en) * | 2020-05-25 | 2020-10-27 | 安徽普偌森环保科技有限公司 | Microbial degradation treatment process for rural domestic sewage |
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