CN108129585A - The purifying plant and method of a kind of agarose - Google Patents
The purifying plant and method of a kind of agarose Download PDFInfo
- Publication number
- CN108129585A CN108129585A CN201711117497.5A CN201711117497A CN108129585A CN 108129585 A CN108129585 A CN 108129585A CN 201711117497 A CN201711117497 A CN 201711117497A CN 108129585 A CN108129585 A CN 108129585A
- Authority
- CN
- China
- Prior art keywords
- agarose
- positive plate
- plate
- purifying plant
- housing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0036—Galactans; Derivatives thereof
- C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention discloses the purifying plants and method of a kind of agarose, the purifying plant of agarose includes housing, DC power supply, multiple positive plates and multiple negative plates, the shell wall of housing is internally provided with heating layer for heating, positive plate is arranged on housing side, negative plate is arranged on housing opposite side, positive plate and negative plate is parallel and cross arrangement, DC power supply for positive plate and negative plate provide can Variable Polarity direct current.The purifying plant of the agarose of the present invention is condenser type, and charge utilization rate is high, can improve the purification efficiency of agar solution, reduces energy consumption.The method of purification of the agarose of the present invention utilizes electrostatic absorption principle, remove the sulfate radical in agar solution, high-efficiency environment friendly, low energy consumption, it is pollution-free, easy to operate implement, purification efficiency it is high, the sulfate radical content of the agarose solution obtained after purification is less than 0.2%, the electroendosmosis of agarose is less than 0.13, can reach purity requirement of the fields such as molecular biology to agarose.
Description
Technical field
The present invention relates to agarose purification technique fields, relate in particular to the purifying plant and method of a kind of agarose.
Background technology
Agar also known as agar-agar are the algal polysaccharides extracted from agar, Eucheuma, fragrant plant mentioned in ancient texts, it by agarose and
Agar-agar ester forms；Wherein agarose is free from the non-ionic polysaccharide of sulfuric ester (salt) as gelling agent, is the group to form gel
Point, and agar-agar ester is non-gel section, is the complicated polysaccharide with sulfuric ester (salt), glucuronic acid and acetone aldehydic acid；Generally
Commodity agar in the sulfuric ester (salt) containing 2%-7%, the pyruvic acid aldehyde of 0%-3% and the first and second bases of 1%-3%.
Agarose has hydrophily, almost neutral, seldom causes denaturation and absorption to sensitive large biological molecule, is
Ideal inert carrier.Agar-agar ester is needed to remove as possible in agarose preparation process, otherwise agarose there may be micro-
Sulfate radical and pyruvic acid substitution ionizing group are measured, causes electroendosmosis (EEO).The agarose in the fields such as molecular biology will to purity
Ask higher, wherein sulfate radical content usually 0.2% hereinafter, electroendosmosis is usually below 0.13.Fine jade is purified from agar at present
The method of lipolysaccharide usually has polyethylene glycol precipitation, DEAE- celluloses method, ethanedioic acid tetraacethyl disodium (EDTA-Na2) method, phosphorus
Sour sodium (Na3PO4) method, sodium iodide (NaI) method etc., above method all has that time-consuming and laborious and purification efficiency is low etc..
Invention content
The purpose of the present invention is to provide the purifying plant and method of a kind of agarose, to solve existing to purify from agar
The problem of time-consuming and laborious and purification efficiency is low existing for the method for agarose.
For this purpose, the present invention provides a kind of purifying plant of agarose, including housing, DC power supply, multiple positive plates and
Multiple negative plates, the shell wall of the housing are internally provided with heating layer for heating, and the positive plate is arranged on the housing one
Side, the negative plate are arranged on the housing opposite side, the positive plate and negative plate is parallel and cross arrangement, described
DC power supply for the positive plate and the negative plate provide can Variable Polarity direct current.
The present invention also provides a kind of method of purification of agarose, the purifying plant that the method for purification uses is the present invention
Purifying plant, the method for purification includes the following steps：
(1) agar solution that mass fraction is 2%-6% is added in the housing and recycled from the import
Flowing, 70-95 DEG C is maintained by the heating layer purifying plant temperature；
(2) positive supply is connected on the positive plate, negative supply is connected on the negative plate so that the agar solution
In sulfate radical adsorb on the positive plate, the operation duration be 5-10min；
(3) connect negative supply on the positive plate, positive supply connected on the negative plate so that adsorb it is described just
Sulfate radical on pole plate comes off, and the operation duration is 2-5min；
(4) step (2) and step (3) are repeated, at least once, the agarose after being purified from exit is molten for number of repetition
The agarose solution is dried to obtain agarose by liquid.
Compared with prior art, the advantages and positive effects of the present invention are：The present invention provides a kind of purifications of agarose
Device and method, the purifying plant of agarose include housing, DC power supply, multiple positive plates and multiple negative plates, the shell of housing
Pars intramuralis is equipped with heating layer for heating, and positive plate is arranged on housing side, and negative plate is arranged on housing opposite side, positive plate
The parallel and cross arrangement with negative plate, DC power supply for positive plate and negative plate provide can Variable Polarity direct current.The present invention
Agarose purifying plant for condenser type, charge utilization rate is high, can improve the purification efficiency of agar solution, reduces energy consumption.
The method of purification of the agarose of the present invention removes the sulfate radical in agar solution, high-efficiency environment friendly, energy consumption using electrostatic absorption principle
Low, pollution-free, easy to operate implementation, purification efficiency are high, and the sulfate radical content of the agarose solution obtained after purification is less than 0.2%,
The electroendosmosis of agarose is less than 0.13, can reach purity requirement of the fields such as molecular biology to agarose.
After the specific embodiment of the present invention is read in conjunction with the figure, the other features and advantages of the invention will become more clear
Chu.
Description of the drawings
Fig. 1 is a kind of structure diagram of embodiment of the purifying plant of agarose of the present invention.
Specific embodiment
The specific embodiment of the present invention is described in detail below, it should be appreciated that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
As shown in Figure 1, the purifying plant of the agarose of the present invention includes housing 1, DC power supply (being not shown), more
A positive plate 2 and multiple negative plates 3, the shell wall of housing 1 are internally provided with heating layer 4 for heating, and positive plate 2 is arranged on housing
1 side, negative plate 3 are arranged on 1 opposite side of housing, positive plate 2 and negative plate 3 is parallel and the arrangement that intersects, DC power supply are
Positive plate 2 and negative plate 3 provide can Variable Polarity direct current.
Purifying plant can be heated by heating layer 4 so that the temperature of purifying plant can be maintained the numerical value of needs.
The quantity of positive plate 2 and negative plate 3 be it is multiple, positive plate 2 it is equal with the quantity of negative plate 3 or difference one
A, in the present embodiment, the quantity of positive plate 2 and negative plate 3 is two.
Positive plate 2 and negative plate 3 are made of conductive porous carbon materials.
Adjacent positive plate 2 is equal with the spacing of negative plate 3, can improve purification efficiency, improves purification effect.
1 inner surface of housing is equipped with insulating layer (being not shown), and insulating layer is made of fabric or fiber grain.
Housing 1 is equipped with import 5 and outlet 6.
Purifying plant of the purifying plant that the method for purification of the agarose of the present invention uses for the present invention, including walking as follows
Suddenly：
(1) agar solution that mass fraction is 2%-6% is added in housing 1 and circulated from import 5, led to
Heating layer 4 is crossed so that purifying plant temperature is maintained 70-95 DEG C；Since agar is macromolecular substances, if agar solution quality point
It if number is excessive, can so that agar solution viscosity is big, eventually so that the refining effect of agar solution is poor, be to mass fraction
The agar solution of 2%-6% is purified, and can so that refining effect is best；Purifying plant temperature is maintained 70-95 DEG C, is had
Circulating and spread conducive to agar solution；
(2) positive supply is connected on positive plate 2, negative supply is connected on negative plate 3 so that the sulfate radical in agar solution
On positive plate 2, the operation duration is 5-10min for absorption；Step (2), will be in agar solution by electrostatic absorption principle
Sulfate radical is adsorbed on positive plate 2, and low energy consumption, charge utilization rate is high, easily regenerates, is pollution-free, is a kind of removal of environment-friendly high-efficiency
The method of sulfate radical in agar solution；
(3) negative supply is connected on positive plate 2, positive supply is connected on negative plate 3 so that adsorbed on positive plate 2
Sulfate radical comes off, and the operation duration is 2-3min；Step (3) comes off the sulfate radical adsorbed on positive plate 2, can improve
Positive plate 2 is to the adsorption capacity of sulfate radical, so as to improve the purification efficiency of agar solution and purification effect；
(4) repeat step (2) and step (3), number of repetition at least once, from the agarose exported after being purified at 6
Agarose solution is dried to obtain agarose by solution.
Step (3) further includes is pumped into distilled water within the case 1 so that distilled water is to adsorbing the sulfate radical on positive plate 2
It is rinsed.
The purifying plant of the agarose of the present invention is condenser type, and charge utilization rate is high, can improve the purification of agar solution
Efficiency reduces energy consumption.The method of purification of the agarose of the present invention removes the sulfuric acid in agar solution using electrostatic absorption principle
Root, high-efficiency environment friendly, low energy consumption, it is pollution-free, easy to operate implement, purification efficiency it is high, the sulfuric acid of the agarose solution obtained after purification
Radical content is less than 0.2%, and the electroendosmosis of agarose is less than 0.13, can reach purity of the fields such as molecular biology to agarose
It is required that.
Embodiment 1
(1) agar solution that mass fraction is 2% is added in housing 1 and circulated from import 5, by adding
Thermosphere 4 is so that purifying plant temperature is maintained 80 DEG C；
(2) positive supply is connected on positive plate 2, negative supply is connected on negative plate 3 so that the sulfate radical in agar solution
On positive plate 2, the operation duration is 8min for absorption；
(3) negative supply is connected on positive plate 2, positive supply is connected on negative plate 3 so that adsorbed on positive plate 2
Sulfate radical comes off, and the operation duration is 3min；
(4) step (2) and step (3) are repeated, the agarose solution after being purified at outlet 6, by agarose solution
It is dried to obtain agarose.
So that sulfate radical content in elemental analyser monitoring agarose solution, it is 0.18% to obtain methyllanthionine with content, is surveyed
The electroendosmosis for measuring agarose is 0.11.
Electroendosmosis measurement process includes：0.45g agarose samples are weighed, add in the barbital buffering of 30mL pH value 8.6
Liquid is swollen 1.5h at room temperature.It is heated to dissolving, supplies ultra-pure water, be configured to 1.5% agarose solution.Glue is poured into while hot
Plate
In, comb is put immediately.After 20min, comb is removed, Ago-Gel is put into togerther electrophoresis tank together with plastic plate
In, the barbitol buffer solution of addition pH value 8.6.Take 6 μ L of sample solution, loading.At room temperature, constant voltage 85V, electrophoresis 2.5h.It takes
Go out agarose offset plate, impregnate 30min by decolorising agent successively, coloring agent impregnates 30min.Then it is impregnated and is vibrated with decolorising agent again
Decolorising agent is replaced 1 time in 3h, centre.Side of the positive electrode blue spot is measured to the distance (OA) in loading home position, negative side leukasmus
Point arrives the distance (OD) in loading home position, and the size of electroendosmosis value m is:
Embodiment 2
(1) agar solution that mass fraction is 6% is added in housing 1 and circulated from import 5, by adding
Thermosphere 4 is so that purifying plant temperature is maintained 80 DEG C；
(2) positive supply is connected on positive plate 2, negative supply is connected on negative plate 3 so that the sulfate radical in agar solution
On positive plate 2, the operation duration is 10min for absorption；
(3) negative supply is connected on positive plate 2, positive supply is connected on negative plate 3 so that adsorbed on positive plate 2
Sulfate radical comes off, and the operation duration is 3min；
(4) step (2) and step (3) are repeated, the agarose solution after being purified at outlet 6, by agarose solution
It is dried to obtain agarose.
So that sulfate radical content in elemental analyser monitoring agarose solution, it is 0.12% to obtain methyllanthionine with content；It surveys
The electroendosmosis for measuring agarose is 0.09, and electroendosmosis measurement process is the same as embodiment 1.
The above embodiments are merely illustrative of the technical solutions of the present invention rather than is limited；Although with reference to aforementioned reality
Example is applied the present invention is described in detail, it for those of ordinary skill in the art, still can be to aforementioned implementation
Technical solution recorded in example modifies or carries out equivalent replacement to which part technical characteristic；And these are changed or replace
It changes, the spirit and scope for claimed technical solution of the invention that it does not separate the essence of the corresponding technical solution.
Claims (9)
1. a kind of purifying plant of agarose, which is characterized in that
Including housing, DC power supply, multiple positive plates and multiple negative plates, the shell wall of the housing is internally provided with for heating
Heating layer, the positive plate are arranged on the housing side, and the negative plate is arranged on the housing opposite side, the positive plate
The parallel and cross arrangement with the negative plate, the DC power supply is provided for the positive plate and the negative plate can Variable Polarity
Direct current.
2. the purifying plant of agarose as described in claim 1, which is characterized in that
The quantity of the positive plate and the negative plate it is equal or difference one.
3. the purifying plant of agarose as described in claim 1, which is characterized in that
The positive plate and the negative plate are made of conductive porous carbon material.
4. the purifying plant of agarose as described in claim 1, which is characterized in that
The adjacent positive plate and the spacing of the negative plate are equal.
5. the purifying plant of agarose as described in claim 1, which is characterized in that
The shell inner surface is equipped with insulating layer, and the insulating layer is made of fabric or fiber grain.
6. the purifying plant of agarose as described in claim 1, which is characterized in that
The housing is equipped with inlet and outlet.
7. a kind of method of purification of agarose, which is characterized in that the purifying plant that the method for purification uses is in embodiment 1-6
Any one of them purifying plant, the method for purification include the following steps：
（1）The agar solution that mass fraction is 2%-6% is added in the housing and circulated from the import, is led to
The heating layer is crossed so that the purifying plant temperature is maintained 70-95 DEG C；
（2）Positive supply is connected on the positive plate, negative supply is connected on the negative plate so that in the agar solution
Sulfate radical is adsorbed on the positive plate, and the operation duration is 5-10min；
（3）Negative supply is connected on the positive plate, positive supply is connected on the negative plate so that is adsorbed in the positive plate
On sulfate radical come off, the operation duration be 2-5min；
（4）Repeat step（2）And step（3）, number of repetition at least once, the agarose solution after being purified from exit,
The agarose solution is dried to obtain agarose.
8. the method for purification of agarose as claimed in claim 7, which is characterized in that
The step（3）It is additionally included in the housing and is pumped into distilled water so that distilled water is to adsorbing on the positive plate
Sulfate radical is rinsed.
9. the method for purification of agarose as claimed in claim 7, which is characterized in that
The sulfate radical content of the agarose solution is less than 0.2%, and the electroendosmosis of the agarose is less than 0.13.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711117497.5A CN108129585A (en) | 2017-11-13 | 2017-11-13 | The purifying plant and method of a kind of agarose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711117497.5A CN108129585A (en) | 2017-11-13 | 2017-11-13 | The purifying plant and method of a kind of agarose |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108129585A true CN108129585A (en) | 2018-06-08 |
Family
ID=62388561
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711117497.5A Pending CN108129585A (en) | 2017-11-13 | 2017-11-13 | The purifying plant and method of a kind of agarose |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108129585A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728583A (en) * | 2018-08-13 | 2018-11-02 | 安吉艾格赛思生物科技有限公司 | A kind of concentrating and purifying device of xylo-oligosaccharide |
| CN108728582A (en) * | 2018-08-13 | 2018-11-02 | 安吉艾格赛思生物科技有限公司 | A kind of equipment efficiently preparing functional low polyxylose |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1023179A (en) * | 1963-08-14 | 1966-03-23 | South African Inventions | Fractionation of mixtures of agarose and agaropectin |
| EP0304024A2 (en) * | 1987-08-17 | 1989-02-22 | FMC Corporation | Agarose purification method using glycol |
| CN1277971A (en) * | 2000-06-02 | 2000-12-27 | 华南理工大学 | Integrated system and method for ultra filtering separation and cold sterilization for mucopolysaccharide |
| CN1821276A (en) * | 2006-02-28 | 2006-08-23 | 北京大学 | Device and its process for extracting hyaluronic acid by electrolemma separating technology |
| CN101195651A (en) * | 2007-06-08 | 2008-06-11 | 桂林医学院 | Method and device for protein compression/purification |
| CN103910811A (en) * | 2014-04-02 | 2014-07-09 | 华侨大学 | Preparation method of agarose with low electroendosmosis |
| CN106008752A (en) * | 2016-07-19 | 2016-10-12 | 厦门太阳马生物工程有限公司 | Method for preparing low electro-endosmose agarose through preparation from agar by chitosan flocculence |
-
2017
- 2017-11-13 CN CN201711117497.5A patent/CN108129585A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1023179A (en) * | 1963-08-14 | 1966-03-23 | South African Inventions | Fractionation of mixtures of agarose and agaropectin |
| EP0304024A2 (en) * | 1987-08-17 | 1989-02-22 | FMC Corporation | Agarose purification method using glycol |
| CN1277971A (en) * | 2000-06-02 | 2000-12-27 | 华南理工大学 | Integrated system and method for ultra filtering separation and cold sterilization for mucopolysaccharide |
| CN1821276A (en) * | 2006-02-28 | 2006-08-23 | 北京大学 | Device and its process for extracting hyaluronic acid by electrolemma separating technology |
| CN101195651A (en) * | 2007-06-08 | 2008-06-11 | 桂林医学院 | Method and device for protein compression/purification |
| CN103910811A (en) * | 2014-04-02 | 2014-07-09 | 华侨大学 | Preparation method of agarose with low electroendosmosis |
| CN106008752A (en) * | 2016-07-19 | 2016-10-12 | 厦门太阳马生物工程有限公司 | Method for preparing low electro-endosmose agarose through preparation from agar by chitosan flocculence |
Non-Patent Citations (2)
| Title |
|---|
| 甘玲等: "《动物生物化学（第1版）》", 28 February 2015, 西南师范大学出版社 * |
| 赵鲁杭等: "《分子医学实验技术（第1版）》", 30 April 2014, 浙江大学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728583A (en) * | 2018-08-13 | 2018-11-02 | 安吉艾格赛思生物科技有限公司 | A kind of concentrating and purifying device of xylo-oligosaccharide |
| CN108728582A (en) * | 2018-08-13 | 2018-11-02 | 安吉艾格赛思生物科技有限公司 | A kind of equipment efficiently preparing functional low polyxylose |
| CN108728583B (en) * | 2018-08-13 | 2024-04-12 | 青岛瑞利恩生物科技有限公司 | Concentration purification device of xylooligosaccharide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yeo et al. | Enhancement of nitrate removal from a solution of mixed nitrate, chloride and sulfate ions using a nitrate-selective carbon electrode | |
| CN105935694B (en) | A kind of repair system of soil Fl object | |
| CN108129585A (en) | The purifying plant and method of a kind of agarose | |
| CN105308317A (en) | Large-capacity electric power storage system using thermal energy/chemical potential | |
| CN106949571A (en) | A kind of electrochemistry dehydrating unit based on screen mesh type amphoteric ion exchange membrane electrode | |
| CN109607711A (en) | A kind of hydridization capacitive deionization desalination module and desalination process | |
| CN107768659A (en) | A kind of preparation method of the sulfur electrode material of loose structure | |
| CN109179589A (en) | The preparation method of carbon coating vanadium phosphate sodium electrode material and its application in hydridization capacitive deionization technology | |
| CN102879255A (en) | Electrically-driven solid phase micro-extraction method and device thereof | |
| CN110417295A (en) | A kind of power generating device based on liquid evaporation | |
| CN102583628A (en) | Method for removing cyanuric acid in water by activated-carbon fibrofelt and for regenerating activated-carbon fibrofelt by electric desorption | |
| CN111250264A (en) | Method and device for electrostatic condensation of wet air | |
| CN112032865B (en) | Falling film type liquid humidity regulator and method based on high-voltage electrostatic field polarization effect | |
| Tabrizizadeh et al. | Empowerment of water-evaporation-induced electric generators via the use of metal electrodes | |
| CN114409031A (en) | Carbon source purification method based on sludge anaerobic fermentation liquid | |
| CN202785762U (en) | Electric adsorption water treatment device | |
| CN113213598A (en) | Ti-MXene derived sodium titanium phosphate/graphene composite material and preparation method and application thereof | |
| CN103910811A (en) | Preparation method of agarose with low electroendosmosis | |
| Yang et al. | Photovoltaic capacitive deionization regeneration method for liquid desiccant cooling system | |
| CN107546038A (en) | A kind of concentration difference capacitor | |
| CN111762769A (en) | Preparation method and application of vanadium sodium oxygen fluorophosphate/graphene composite electrode material | |
| CN206563376U (en) | A kind of electrochemistry dehydrating unit based on screen mesh type amphoteric ion exchange membrane electrode | |
| CN102580539B (en) | Method for simulating natural acidification of soil | |
| CN113587269A (en) | Solution dehumidification/regenerator based on electrostatic field interface enhancement | |
| CN108314229A (en) | Heavy metal in water ion based on nanopore device gradually partition method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180608 |
|
| RJ01 | Rejection of invention patent application after publication |