CN108120696A - A kind of discrimination method of the big roundleaf roxburgh anoectochilus terminal bud of different planting - Google Patents
A kind of discrimination method of the big roundleaf roxburgh anoectochilus terminal bud of different planting Download PDFInfo
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- 241000719837 Anoectochilus Species 0.000 title claims abstract description 52
- 238000012850 discrimination method Methods 0.000 title claims abstract description 18
- 239000002689 soil Substances 0.000 claims abstract description 22
- 241000196324 Embryophyta Species 0.000 claims abstract description 18
- 238000002329 infrared spectrum Methods 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 10
- 238000005516 engineering process Methods 0.000 claims abstract description 6
- 238000012937 correction Methods 0.000 claims abstract description 5
- 238000009795 derivation Methods 0.000 claims abstract description 5
- 239000000843 powder Substances 0.000 claims abstract description 5
- 238000000205 computational method Methods 0.000 claims abstract description 3
- 238000010183 spectrum analysis Methods 0.000 claims abstract 2
- 238000012545 processing Methods 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 5
- 240000002853 Nelumbo nucifera Species 0.000 claims description 2
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims description 2
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 2
- 239000010931 gold Substances 0.000 claims description 2
- 229910052737 gold Inorganic materials 0.000 claims description 2
- 239000004615 ingredient Substances 0.000 claims 1
- 238000007873 sieving Methods 0.000 claims 1
- 230000001681 protective effect Effects 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 54
- 238000001228 spectrum Methods 0.000 description 14
- 238000004458 analytical method Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000934230 Anoectochilus roxburghii Species 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 230000000739 chaotic effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000006101 laboratory sample Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
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- General Health & Medical Sciences (AREA)
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- Investigating Or Analysing Materials By Optical Means (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of big roundleaf roxburgh anoectochilus terminal bud discrimination methods of different planting, it is differentiated using near-infrared spectral analysis technology, it is characterised in that：It includes the following steps：(1) measuring samples powder is taken, fills sample, gathers atlas of near infrared spectra；(2) PCA Cluster clustering methodologies are used as computational methods, choose unit length normalized (nle), first derivation (db1), multiplicative scatter correction (mf) carry out pretreatment atlas of near infrared spectra, the big roundleaf roxburgh anoectochilus terminal bud of different planting is identified with the score value of sample principal component.The discrimination method of the present invention has many advantages, such as easy, quick, accurate, environmentally protective and lossless sample, can effectively differentiate the big roundleaf roxburgh anoectochilus terminal bud that 3 months bottle seedlings, 4 months bottle seedlings or 6 monthly income soil species plant seedlings, application prospect is good.
Description
Technical field
The present invention relates to the discrimination methods of the big roundleaf roxburgh anoectochilus terminal bud of different planting.
Background technology
Roxburgh anoectochilus terminal bud (Anoectochilus roxburghii) is the plant of orchid family Anoectochilus Blume, is mainly distributed on China Fujian
Tai Area is perennial Precious, Rare, Endangered medicinal material.Roxburgh anoectochilus terminal bud has effects that clearing heat and cooling blood, expelling wind and removing dampness, cures mainly ephritis, bladder
The illnesss such as inflammation, diabetes, bronchitis, pediatric acute convulsion.Roxburgh anoectochilus terminal bud has higher medicinal health value, but wild resource is dilute
It lacks, currently on the market based on cultivation product, the strain of circulation mainly includes sharp leaf, big roundleaf, small roundleaf, red rosy clouds and TaiWan, China
Roxburgh anoectochilus terminal bud.Planting type is broadly divided into tissue cultures and plantation of burying, and tissue culture product are mostly the tissue-culture container seedling of 3~5 months, enter soil species
Plant product are mostly that the bottle seedling of 3~5 months is transplanted to the kind of regrowth 3~6 months in soil environment and plants seedlings.Different planting types pair
Active ingredient-polysaccharide of roxburgh anoectochilus terminal bud, the content of flavonoids are affected, and the planting type of tissue culture+plantation of burying is than simple tissue culture
Polysaccharide, flavones content higher, the implantation time that buries is longer, and the residual quantity of heavy metal, hormone and antibiotic is lower, but dead
Rate is also constantly soaring；Therefore not only quality is different for the roxburgh anoectochilus terminal bud of different planting, price also differs greatly.
At present, the research of China's roxburgh anoectochilus terminal bud quality standard is relatively weak, lacks unified product quality inspection standard.
Differentiate that the roxburgh anoectochilus terminal bud of different planting is limited only to judge by rule of thumb in the market, however 3 months bottle seedlings, 4 months bottle seedlings or 6
Difference between a monthly income soil species plants seedlings is not notable, the illegal businessman in part is caused directly to launch tissue-culture container seedling, with secondary
It substitutes the bad for the good, causes selling market chaotic, seriously hinder sound development and the popularization of roxburgh anoectochilus terminal bud industry.
Near-infrared spectrum technique (NIRS) is used as a kind of high-new analytical technology, with finding speed is fast, sample is lossless, does not disappear
The advantages that consuming chemical reagent.In recent years, near-infrared spectrum technique is progressively widely used in Chinese traditional medicine identification field, can quickly reflect
The kind of other Chinese medicine, the true and false mix puppet, place of production etc..
A kind of easy, green, the method for the big roundleaf roxburgh anoectochilus terminal bud for accurately differentiating different planting need to be found.In invention
Hold
The present invention provides a kind of big roundleaf roxburgh anoectochilus terminal bud discrimination method of different planting, it is using near infrared spectrum point
Analysis technology differentiates, it is characterised in that：It includes the following steps：
(1) measuring samples powder is taken, fills sample, gathers atlas of near infrared spectra；
(2) PCA-Cluster clustering methodologies are used as computational methods, choose unit length normalized (nle), single order
Derivation (db1), multiplicative scatter correction (mf) carry out pretreatment atlas of near infrared spectra, are identified with the score value of sample principal component
The big roundleaf roxburgh anoectochilus terminal bud of different planting.
Wherein, the big roundleaf roxburgh anoectochilus terminal bud of the different planting is：3 months bottle seedlings, 4 months bottle seedlings or 6 monthly incomes
Soil species plants seedlings.
Wherein, 3 months bottle seedlings are the big roundleaf roxburgh anoectochilus terminal bud tissue-cultured seedling of 3 months；4 months bottle seedlings are 4
The big roundleaf roxburgh anoectochilus terminal bud tissue-cultured seedling of the moon；6 monthly income soil species plant seedlings to be transplanted for the big roundleaf roxburgh anoectochilus terminal bud tissue-cultured seedling of 3-5 months
The big roundleaf anoectochilus blume seedling of regrowth 6 months into soil environment.
Wherein, the processing step of the sample to be tested powder described in step (1) includes：Roxburgh anoectochilus terminal bud is cleaned up, 55-65 DEG C
It dries, crush, crossing 60-100 mesh sieves, loading refers to pipe, height of specimen 1-2cm.
Wherein, the drying temperature is 60 DEG C；The grit number of crossing is 60 mesh.
Wherein, the acquisition parameter of the atlas of near infrared spectra described in step (1) is：Scanning range is 5000-10000cm-1,
Scanning times are 32 times, resolution ratio 8cm-1。
Wherein, in step (2), the principal component is PC1, PC2.
Wherein, when the principal component is PC1, bottle seedling is scored within 3 months：- 0.189241~0.106634；4 months bottles
Seedling is scored at：0.0404069~0.202613；6 months kinds, which are planted seedlings, to be scored at：- 0.222728~0.0226591；It is described
When principal component is PC2, bottle seedling is scored within 3 months：- 0.32442~0.0128326；Bottle seedling is scored within 4 months：-
0.0300298~0.148264；6 months kinds, which are planted seedlings, to be scored at：- 0.0286609~0.245025.
Wherein, when the score value of the principal component PC1 and PC2 of sample are fallen into the scope in Fig. 1 or Fig. 2 shown in a-quadrant, sample
Product are 3 months bottle seedlings；
When the score value of the principal component PC1 and PC2 of sample are fallen into the scope in Fig. 1 or Fig. 2 shown in B area, sample 4
A month bottle seedling；
When the score value of the principal component PC1 and PC2 of sample are fallen into the scope in Fig. 1 or Fig. 2 shown in C regions, sample 6
A monthly income soil species plants seedlings.
The discrimination method of the present invention has many advantages, such as easy, quick, accurate, environmentally protective and lossless sample, can be effective
Differentiate the big roundleaf roxburgh anoectochilus terminal bud that 3 months bottle seedlings, 4 months bottle seedlings or 6 monthly income soil species plant seedlings, application prospect is good.
Obviously, the above according to the present invention according to the ordinary technical knowledge and customary means of this field, is not departing from
Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification, replacement or change of other diversified forms can also be made.
The specific embodiment of form by the following examples remakes further specifically the above of the present invention
It is bright.But the scope that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to following example.It is all to be based on the above of the present invention
The technology realized all belongs to the scope of the present invention.
Description of the drawings
The principal component two dimension shot chart of the big roundleaf roxburgh anoectochilus terminal bud of Fig. 1 different plantings；
The principal component three-dimensional shot chart of the big roundleaf roxburgh anoectochilus terminal bud of Fig. 2 different plantings.
Specific embodiment
The present invention is by collecting the big roundleaf gold that 3 months bottle seedlings, 4 months bottle seedlings and 6 monthly income soil species of up to a hundred batches are planted seedlings
Line lotus obtains the principal component two dimension of different planting roxburgh anoectochilus terminal bud, three-dimensional shot chart using NIRS-PCA-Cluster methods, point
Not as shown in Figure 1 and Figure 2.When the score value of the principal component PC1 and PC2 of sample fall into the scope in Fig. 1 or Fig. 2 shown in a-quadrant
Interior, sample is 3 months bottle seedlings；When the score value of the principal component PC1 and PC2 of sample fall into the model in Fig. 1 or Fig. 2 shown in B area
In enclosing, sample is 4 months bottle seedlings；When the score value of the principal component PC1 and PC2 of sample are fallen into Fig. 1 or Fig. 2 shown in C regions
In the range of, sample plants seedlings for 6 monthly income soil species.
The detection method of 1 present invention of embodiment
(1) sample treatment：Fresh sample to be tested is cleaned in batches and becomes clear, sample surfaces without silt to water 3~5 times, is placed in 60
DEG C oven for drying crushed 60 mesh sieves to constant weight, and each batch takes appropriate be packed into refer to pipe and tamping makes height of specimen be treated between 2cm
It surveys；
(2) spectra collection and pretreatment：Sample, acquisition parameter are scanned with NIRFlex N-500 near-infrared spectrometers：
Scanning range is 5000-10000cm-1, scanning times are 32 times, resolution ratio 8cm-1, repeatedly dress sample and the scanning 3 of each sample
It is secondary, the sample referred in pipe is tamped before scanning every time, makes sample uniform ground, system is automatically by the NIRS spectrum of scanned sample
It is stored in spectra database；After importing spectrum in 5.4 data processing softwares of NIRCal, by spectrum by unit length normalized
(nle), first derivation (db1), multiplicative scatter correction (mf) processing, obtain and finally analyze spectrum；
(3) sample to be tested differentiates：Analysis spectrum is used into PCA-Cluster clustering methodologies, with sample principal component
The score value of PC1, PC2 identify the big roundleaf roxburgh anoectochilus terminal bud of different planting.When principal component is PC1, the score of 3 months bottle seedlings
For：- 0.189241~0.106634；Bottle seedling is scored within 4 months：0.0404069~0.202613；What 6 months kinds were planted seedlings
It is divided into：- 0.222728~0.0226591；When principal component is PC2, bottle seedling is scored within 3 months：- 0.32442~
0.0128326；Bottle seedling is scored within 4 months：- 0.0300298~0.148264；6 months kinds, which are planted seedlings, to be scored at：-
0.0286609~0.245025.
When the score value of the principal component PC1 and PC2 of sample are fallen into the scope in Fig. 1 or Fig. 2 shown in a-quadrant, sample 3
A month bottle seedling；
When the score value of the principal component PC1 and PC2 of sample are fallen into the scope in Fig. 1 or Fig. 2 shown in B area, sample 4
A month bottle seedling；
When the score value of the principal component PC1 and PC2 of sample are fallen into the scope in Fig. 1 or Fig. 2 shown in C regions, sample 6
A monthly income soil species plants seedlings.
Preferably, the big roundleaf roxburgh anoectochilus terminal bud of the different planting is：3 months bottle seedlings, 4 months bottle seedlings or 6 months
Kind of burying is planted seedlings, and 3 months bottle seedlings are the big roundleaf roxburgh anoectochilus terminal bud tissue-cultured seedling of 3 months；4 months bottle seedlings are the great circle of 4 months
Leaf roxburgh anoectochilus terminal bud tissue-cultured seedling；6 monthly income soil species plant seedlings was transplanted to soil for the big roundleaf roxburgh anoectochilus terminal bud tissue-cultured seedling of 3-5 months
The big roundleaf anoectochilus blume seedling of regrowth 6 months in environment.
The detection method of 2 present invention of embodiment
(1) sample treatment：Fresh sample to be tested is cleaned in batches and becomes clear, sample surfaces without silt to water 3~5 times, is placed in 55
DEG C oven for drying crushed 100 mesh sieves to constant weight, and each batch takes appropriate be packed into refer to pipe and tamping makes height of specimen be treated between 1cm
It surveys；
(2) spectra collection and pretreatment：Sample, acquisition parameter are scanned with NIRFlex N-500 near-infrared spectrometers：
Scanning range is 5000-10000cm-1, scanning times are 32 times, resolution ratio 8cm-1, repeatedly dress sample and the scanning 3 of each sample
It is secondary, the sample referred in pipe is tamped before scanning every time, makes sample uniform ground, system is automatically by the NIRS spectrum of scanned sample
It is stored in spectra database；After importing spectrum in 5.4 data processing softwares of NIRCal, by spectrum by unit length normalized
(nle), first derivation (db1), multiplicative scatter correction (mf) processing, obtain and finally analyze spectrum；
(3) sample to be tested differentiates：Analysis spectrum is used into PCA-Cluster clustering methodologies, with sample principal component
The score value of PC1, PC2 identify the big roundleaf roxburgh anoectochilus terminal bud of different planting.When principal component is PC1, the score of 3 months bottle seedlings
For：- 0.189241~0.106634；Bottle seedling is scored within 4 months：0.0404069~0.202613；What 6 months kinds were planted seedlings
It is divided into：- 0.222728~0.0226591；When principal component is PC2, bottle seedling is scored within 3 months：- 0.32442~
0.0128326；Bottle seedling is scored within 4 months：- 0.0300298~0.148264；6 months kinds, which are planted seedlings, to be scored at：-
0.0286609~0.245025.
When the score value of sample principal component PC1 and PC2 are fallen into the scope in Fig. 1 or Fig. 2 shown in a-quadrant, sample is 3
Month bottle seedling；
When the score value of the principal component PC1 and PC2 of sample are fallen into the scope in Fig. 1 or Fig. 2 shown in B area, sample 4
A month bottle seedling；
When the score value of the principal component PC1 and PC2 of sample are fallen into the scope in Fig. 1 or Fig. 2 shown in C regions, sample 6
A monthly income soil species plants seedlings.
Preferably, the big roundleaf roxburgh anoectochilus terminal bud of the different planting is：3 months bottle seedlings, 4 months bottle seedlings or 6 months
Kind of burying is planted seedlings, and 3 months bottle seedlings are the big roundleaf roxburgh anoectochilus terminal bud tissue-cultured seedling of 3 months；4 months bottle seedlings are the great circle of 4 months
Leaf roxburgh anoectochilus terminal bud tissue-cultured seedling；6 monthly income soil species plant seedlings was transplanted to soil for the big roundleaf roxburgh anoectochilus terminal bud tissue-cultured seedling of 3-5 months
The big roundleaf anoectochilus blume seedling of regrowth 6 months in environment.
Beneficial effects of the present invention are proved below by way of specific identification experiment：
The discriminating of the big roundleaf roxburgh anoectochilus terminal bud of 1 different planting of experimental example
1 experiment material
1.1 instrument
NIRFlex N-500 near-infrared spectrometers, solid measuring cell, attachment refer to pipe for 6, and sample spinner is overflow instead
Penetrate integrating sphere, 5.4 software data processings of NIRCal and modeling (Buchi Labortechnik AG companies).
The desk-top continuous dosing pulverizers of DF-15 (Lin great Machinery Co., Ltd.s of Wenling city), 60 mesh standard inspection sieves (60 mesh holes
Footpath, 0.3mm, milky way test equipment factory of Shangyu city), DHG-9030H constant temperature ovens (the upper grand experimental facilities Co., Ltd of Nereid).
1.2 laboratory sample
3 months bottle seedlings of acquisition, 4 months bottle seedlings, 6 months kinds are planted seedlings each 10 batches；Sample source is in Nan Jing's Ge Yuan agriculture and forestry science and technologies
Co., Ltd.3 months bottle seedlings were section with 3 months, and 4 months bottle seedlings were section with 4 months, and 6 months kinds are planted seedlings as 5 months bottle seedlings
It is section to be transplanted in soil environment regrowth 6 months..
2 detection methods
Method using embodiment 1 is differentiated.
3 testing results
It the results are shown in Table 1
The discriminating accuracy of the big roundleaf roxburgh anoectochilus terminal bud of 1 different planting of table
Judged according to the principal component two dimension shot chart of the big roundleaf roxburgh anoectochilus terminal bud of different planting, principal component graphics.As a result
Such as table 1, plant seedlings each 10 batches 3 months bottle seedlings, 4 months bottle seedlings, 6 months kinds and test with discrimination method of the present invention, accurately
Rate is respectively 100%, 80% and 90%.
To sum up, discrimination method of the invention has many advantages, such as easy, quick, accurate, environmentally protective and lossless sample, can be with
Effectively differentiate the big roundleaf roxburgh anoectochilus terminal bud that 3 months bottle seedlings, 4 months bottle seedlings or 6 monthly income soil species plant seedlings, application prospect is good.
Claims (9)
1. a kind of big roundleaf roxburgh anoectochilus terminal bud discrimination method of different planting, it is differentiated using near-infrared spectral analysis technology,
It is characterized in that：It includes the following steps：
(1) measuring samples powder is taken, fills sample, gathers atlas of near infrared spectra；
(2) PCA-Cluster clustering methodologies are used as computational methods, choose unit length normalized (nle), first derivation
(db1), multiplicative scatter correction (mf) carries out pretreatment atlas of near infrared spectra, and difference is identified with the score value of sample principal component
The big roundleaf roxburgh anoectochilus terminal bud of planting type.
2. discrimination method according to claim 1, it is characterised in that：The big roundleaf roxburgh anoectochilus terminal bud of the different planting
For：3 months bottle seedlings, 4 months bottle seedlings or 6 monthly income soil species plant seedlings.
3. discrimination method according to claim 2, it is characterised in that：The big roundleaf gold that 3 months bottle seedlings are 3 months
Line lotus tissue-cultured seedling；4 months bottle seedlings are the big roundleaf roxburgh anoectochilus terminal bud tissue-cultured seedling of 4 months；6 monthly income soil species plant seedlings for
The big roundleaf roxburgh anoectochilus terminal bud tissue-cultured seedling of 3-5 months is transplanted to the big roundleaf anoectochilus blume seedling of regrowth 6 months in soil environment.
4. discrimination method according to claim 1, it is characterised in that：The processing of sample to be tested powder described in step (1)
Step includes：Roxburgh anoectochilus terminal bud is cleaned up, 55-65 DEG C of drying, crushes, cross 60-100 mesh sieves, being packed into and refer to pipe, height of specimen 1-
2cm。
5. discrimination method according to claim 4, it is characterised in that：The drying temperature is 60 DEG C；The sieving
Mesh number is 60 mesh.
6. discrimination method according to claim 1, it is characterised in that：The acquisition of atlas of near infrared spectra described in step (1)
Parameter is：Scanning range is 5000-10000cm-1, scanning times are 32 times, resolution ratio 8cm-1。
7. discrimination method according to claim 1, it is characterised in that：In step (2), the principal component is PC1, PC2.
8. discrimination method according to claim 7, it is characterised in that：
When the principal component is PC1, bottle seedling is scored within 3 months：- 0.189241~0.106634；The score of 4 months bottle seedlings
For：0.0404069~0.202613；6 months kinds, which are planted seedlings, to be scored at：- 0.222728~0.0226591；
When the principal component is PC2, bottle seedling is scored within 3 months：- 0.32442~0.0128326；The score of 4 months bottle seedlings
For：- 0.0300298~0.148264；6 months kinds, which are planted seedlings, to be scored at：- 0.0286609~0.245025.
9. the discrimination method according to claim 7 or 8, it is characterised in that：
When the score value of the ingredient PC1 and PC2 of sample master are fallen into the scope in Fig. 1 or Fig. 2 shown in a-quadrant, sample is 3 months
Bottle seedling；
When the score value of the principal component PC1 and PC2 of sample are fallen into the scope in Fig. 1 or Fig. 2 shown in B area, sample is 4 months
Bottle seedling；
When the score value of the principal component PC1 and PC2 of sample are fallen into the scope in Fig. 1 or Fig. 2 shown in C regions, sample is 6 months
Kind of burying is planted seedlings.
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| 王庆振等: "近红外漫反射光纤光谱法快速鉴别新疆黑果小檗皮", 《安徽医药》 * |
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