CN108118076A - A kind of method for improving Cordyceps militaris production cordycepin - Google Patents
A kind of method for improving Cordyceps militaris production cordycepin Download PDFInfo
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- CN108118076A CN108118076A CN201611058384.8A CN201611058384A CN108118076A CN 108118076 A CN108118076 A CN 108118076A CN 201611058384 A CN201611058384 A CN 201611058384A CN 108118076 A CN108118076 A CN 108118076A
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
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- C12P19/40—Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
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Abstract
The invention belongs to cordyceps sinensis preparing technical fields, are related to a kind of method for improving Cordyceps militaris production cordycepin.A kind of method for improving Cordyceps militaris production cordycepin, step are as follows：Potato is cooked and filters to take juice, again by 10 15g of glucose, 15 18g of sucrose, 0.5 1.0g of magnesium sulfate, 1 2g of potassium sulfate, 2 3g of peptone, 0.5 1g of yeast extract, 18 20g of agar, cocoon pupa juice 200 300ml and 800 1000mL of water are uniformly mixed with potato juice and are configured to strain activation and culture base；With obtained mother culture media；It adds in adenine and adenosine prepares liquid fermentation basal medium；The sterile access Cordyceps militaris spawn in strain activation and culture base is 25 35 DEG C of 1 1.5d of standing in temperature, is put into shaken cultivation case culture；Liquid spawn is inoculated in the mother culture media after sterilizing and cultivates 3 5d, it places into liquid fermentation basal medium to cultivate and be cultivated in 8 10d, it gets product, using addition adenine and the mixing additive of adenosine, cordycepin output and easy to operate can be improved by adding in fluid nutrient medium for preparation method of the present invention.
Description
Technical field
The invention belongs to cordyceps sinensis preparing technical fields, are related to a kind of method for improving Cordyceps militaris production cordycepin.
Background technology
Cordycepin has multiple biological activities, there is the effects that antibacterial, reducing blood lipid and anticancer.Cordyceps militaris is main production worm at present
The bacterial strain of careless element, the liquid fermentation research of Cordyceps militaris are concentrated mainly on the optimization of fermentation condition and the context of detection of active ingredient.
And it is very few for the Dynamic Accumulation research of active ingredient in fermentation process, in industrial production, the maximum of target product should be obtained
Amount will also save production cost and there are the sporophore growth cycle is long, and the content of cordycepin is low, and extraction cordycepin is of high cost etc.
Shortcoming, therefore, developing a kind of method for improving Cordyceps militaris production cordycepin is very important.
The content of the invention
In view of the problems of the existing technology, it is an object of the invention to provide a kind of raising Cordyceps militaris production cordyceps sinensis
The method of rhzomorph.Using addition adenine and the mixing additive of adenosine, adding in fluid nutrient medium can carry preparation method of the present invention
High cordycepin yield and easy to operate.
In order to solve the above technical problems, the technical solution adopted by the present invention is.
A kind of method for improving Cordyceps militaris production cordycepin, step are as follows：
（1）Potato 150-200g and 1000-1500ml water is put into vessel in heating to be cooked, filters to take juice 500-700ml, then
By glucose 10-15g, sucrose 15-18g, magnesium sulfate 0.5-1.0g, potassium sulfate 1-2g, peptone 2-3g, yeast extract 0.5-1g,
Agar 18-20g, cocoon pupa juice 200-300ml and water 800-1000mL are uniformly mixed with potato juice is configured to actication of culture training
Support base；
（2）Weigh glucose 5-15g, sucrose 10-15g, peptone 1-3g, yeast extract 1-2g, magnesium sulfate 0.2-1.0g, sulfuric acid
Potassium 0.5-1.0g and water 800-1000mL is put into container and is uniformly mixed, it is sterilized in the case where temperature is 120-125 DEG C 25-
35min, which matches somebody with somebody, is made mother culture media；
（3）Weigh glucose 5-15g, brown sugar 10-15g, peptone 1-3g, yeast extract 1-2g, magnesium sulfate 0.2-1.0g, sulfuric acid
Potassium 0.5-1.0g and water 800-1000mL is put into container and is uniformly mixed, and addition gland is fast after removing impurity with 0 .25um microfiltration membranes
Purine and adenosine are adjusted to mass concentration 0.9-1.2g/L, it is sterilized 25-35min with obtained liquid in the case where temperature is 120-125 DEG C
Ferment basal medium；
（4）It is 25-35 DEG C of standing 1- in temperature by sterile working access for examination Cordyceps militaris spawn in strain activation and culture base
1.5d places into shaken cultivation case, and in the case where temperature is 25-35 DEG C, rotating speed is cultivated under the conditions of being 120-140r/min, obtains liquid
Strain；
（5）Liquid spawn is inoculated in the mother culture media after sterilizing and cultivates 3-5d, places into liquid fermentation basal medium
Culture is to get finished product in middle culture 8-10d.
The step（3）In adenine and adenosine mass ratio be 1-2:3-5.
Beneficial effects of the present invention：The present invention adds in Liquid Culture using addition adenine and the mixing additive of adenosine
Base can improve cordycepin output.
Specific embodiment
For a further understanding of the present invention, the preferred embodiment of the invention is described with reference to embodiment, still
It should be appreciated that these descriptions are simply for the feature and advantage that further illustrate the present invention rather than to the claims in the present invention
Limitation.
Embodiment 1
A kind of method for improving Cordyceps militaris production cordycepin, step are as follows：
（1）Potato 180g and 1200ml water is put into vessel in heating to be cooked, filters to take juice 600ml, then by glucose 12g,
Sucrose 16g, magnesium sulfate 0.8g, potassium sulfate 1g, peptone 2g, yeast extract 0.5g, agar 18g, cocoon pupa juice 280ml and water
900mL is uniformly mixed with potato juice is configured to strain activation and culture base；
（2）Weigh glucose 8g, sucrose 12g, peptone 2g, yeast extract 1g, magnesium sulfate 0.8g, potassium sulfate 0.7g and water 900mL
It is put into container and is uniformly mixed, it is sterilized into 25-35min with obtained mother culture media in the case where temperature is 120-125 DEG C；
（3）Weigh glucose 12g, brown sugar 12g, peptone 2g, yeast extract 1g, magnesium sulfate 0.6g, potassium sulfate 0.8g and water
1000mL is put into container and is uniformly mixed, and adenine and adenosine, adenine and gland are added in after removing impurity with 0 .25um microfiltration membranes
Glycosides mass ratio is 1:3, mass concentration 1.0g/L is adjusted to, it is sterilized into 25-35min with obtained liquid in the case where temperature is 120-125 DEG C
Body fermentation basal medium；
（4）It is 25-35 DEG C of standing 1d in temperature by sterile working access for trying Cordyceps militaris spawn in strain activation and culture base,
Shaken cultivation case is placed into, in the case where temperature is 25-35 DEG C, rotating speed is cultivated under the conditions of being 120-140r/min, obtains liquid spawn；
（5）Liquid spawn is inoculated in the mother culture media after sterilizing and cultivates 4d, is placed into liquid fermentation basal medium
It cultivates in 9d and cultivates to get finished product.
Embodiment 2
A kind of method for improving Cordyceps militaris production cordycepin, step are as follows：
（1）Potato 190g and 1400ml water is put into vessel in heating to be cooked, filters to take juice 600ml, then by glucose 13g,
Sucrose 17g, magnesium sulfate 0.8g, potassium sulfate 1.5g, peptone 2.2g, yeast extract 0.8g, agar 19g, cocoon pupa juice 240ml and water
1000mL is uniformly mixed with potato juice is configured to strain activation and culture base；
（2）Weigh glucose 9g, sucrose 14g, peptone 2g, yeast extract 2g, magnesium sulfate 0.8g, potassium sulfate 0.6g and water
1000mL is put into container and is uniformly mixed, it is sterilized 25-35min with obtained Mother culture in the case where temperature is 120-125 DEG C
Base；
（3）Weigh glucose 15g, brown sugar 15g, peptone 2g, yeast extract 1g, magnesium sulfate 0.9g, potassium sulfate 0.8g and water
880mL is put into container and is uniformly mixed, and adenine and adenosine, adenine and gland are added in after removing impurity with 0 .25um microfiltration membranes
Glycosides mass ratio is 2:5, mass concentration 1.2g/L is adjusted to, it is sterilized into 25-35min with obtained liquid in the case where temperature is 120-125 DEG C
Body fermentation basal medium；
（4）It is 25-35 DEG C of standing 1d in temperature by sterile working access for trying Cordyceps militaris spawn in strain activation and culture base,
Shaken cultivation case is placed into, in the case where temperature is 25-35 DEG C, rotating speed is cultivated under the conditions of being 120-140r/min, obtains liquid spawn；
（5）Liquid spawn is inoculated in the mother culture media after sterilizing and cultivates 5d, is placed into liquid fermentation basal medium
It cultivates in 10d and cultivates to get finished product.
Embodiment 3
A kind of method for improving Cordyceps militaris production cordycepin, step are as follows：
（1）Potato 200g and 1500ml water is put into vessel in heating to be cooked, filters to take juice 700ml, then by glucose 14g,
Sucrose 17g, magnesium sulfate 0.9g, potassium sulfate 2g, peptone 3g, yeast extract 0.6g, agar 20g, cocoon pupa juice 300ml and water
1000mL is uniformly mixed with potato juice is configured to strain activation and culture base；
（2）Weigh glucose 14g, sucrose 11g, peptone 2g, yeast extract 1.5g, magnesium sulfate 0.7g, potassium sulfate 0.5-1.0g and
Water 9000mL is put into container and is uniformly mixed, it is sterilized 25-35min with obtained Mother culture in the case where temperature is 120-125 DEG C
Base；
（3）Weigh glucose 12g, brown sugar 13g, peptone 2g, yeast extract 1g, magnesium sulfate 0.4g, potassium sulfate 0.7g and water
850mL is put into container and is uniformly mixed, and adenine and adenosine, adenine and gland are added in after removing impurity with 0 .25um microfiltration membranes
Glycosides mass ratio is 1:3, mass concentration 1.1g/L is adjusted to, it is sterilized into 25-35min with obtained liquid in the case where temperature is 120-125 DEG C
Body fermentation basal medium；
（4）It is 25-35 DEG C of standing in temperature by sterile working access for examination Cordyceps militaris spawn in strain activation and culture base
1.3d places into shaken cultivation case, and in the case where temperature is 25-35 DEG C, rotating speed is cultivated under the conditions of being 120-140r/min, obtains liquid
Strain；
（5）Liquid spawn is inoculated in the mother culture media after sterilizing and cultivates 4d, is placed into liquid fermentation basal medium
It cultivates in 9d and cultivates to get finished product.
Embodiment 4
A kind of method for improving Cordyceps militaris production cordycepin, step are as follows：
（1）Potato 1950g and 1400ml water is put into vessel in heating to be cooked, filters to take juice 600ml, then by glucose 13g,
Sucrose 16g, magnesium sulfate 0.7g, potassium sulfate 1g, peptone 2.6g, yeast extract 0.9g, agar 19g, cocoon pupa juice 300ml and water
980mL is uniformly mixed with potato juice is configured to strain activation and culture base；
（2）Weigh glucose 8g, sucrose 12g, peptone 1g, yeast extract 2g, magnesium sulfate 0.5g, potassium sulfate 0.9g and water 890mL
It is put into container and is uniformly mixed, it is sterilized into 25-35min with obtained mother culture media in the case where temperature is 120-125 DEG C；
（3）Weigh glucose 11g, brown sugar 12g, peptone 2g, yeast extract 1g, magnesium sulfate 0.5g, potassium sulfate 0.9g and water
1000mL is put into container and is uniformly mixed, and adenine and adenosine, adenine and gland are added in after removing impurity with 0 .25um microfiltration membranes
Glycosides mass ratio is 2:3, mass concentration 0.9g/L is adjusted to, it is sterilized into 25-35min with obtained liquid in the case where temperature is 120-125 DEG C
Body fermentation basal medium；
（4）It is 25-35 DEG C of standing in temperature by sterile working access for examination Cordyceps militaris spawn in strain activation and culture base
1.5d places into shaken cultivation case, and in the case where temperature is 25-35 DEG C, rotating speed is cultivated under the conditions of being 120-140r/min, obtains liquid
Strain；
（5）Liquid spawn is inoculated in the mother culture media after sterilizing and cultivates 5d, is placed into liquid fermentation basal medium
It cultivates in 10d and cultivates to get finished product.
It is understood that above with respect to the specific descriptions of the present invention, it is merely to illustrate the present invention and is not limited to this
The described technical solution of inventive embodiments, it will be understood by those of ordinary skill in the art that, still the present invention can be carried out
Modification or equivalent substitution, to reach identical technique effect；As long as meeting using needs, all protection scope of the present invention it
It is interior.
Claims (2)
- A kind of 1. method for improving Cordyceps militaris production cordycepin, which is characterized in that step is as follows：（1）Potato 150-200g and 1000-1500ml water is put into vessel in heating to be cooked, filters to take juice 500-700ml, then By glucose 10-15g, sucrose 15-18g, magnesium sulfate 0.5-1.0g, potassium sulfate 1-2g, peptone 2-3g, yeast extract 0.5-1g, Agar 18-20g, cocoon pupa juice 200-300ml and water 800-1000mL are uniformly mixed with potato juice is configured to actication of culture training Support base；（2）Weigh glucose 5-15g, sucrose 10-15g, peptone 1-3g, yeast extract 1-2g, magnesium sulfate 0.2-1.0g, sulfuric acid Potassium 0.5-1.0g and water 800-1000mL is put into container and is uniformly mixed, it is sterilized in the case where temperature is 120-125 DEG C 25- 35min, which matches somebody with somebody, is made mother culture media；（3）Weigh glucose 5-15g, brown sugar 10-15g, peptone 1-3g, yeast extract 1-2g, magnesium sulfate 0.2-1.0g, sulfuric acid Potassium 0.5-1.0g and water 800-1000mL is put into container and is uniformly mixed, and addition gland is fast after removing impurity with 0 .25um microfiltration membranes Purine and adenosine are adjusted to mass concentration 0.9-1.2g/L, it is sterilized 25-35min with obtained liquid in the case where temperature is 120-125 DEG C Ferment basal medium；（4）It is 25-35 DEG C of standing 1- in temperature by sterile working access for examination Cordyceps militaris spawn in strain activation and culture base 1.5d places into shaken cultivation case, and in the case where temperature is 25-35 DEG C, rotating speed is cultivated under the conditions of being 120-140r/min, obtains liquid Strain；（5）Liquid spawn is inoculated in the mother culture media after sterilizing and cultivates 3-5d, places into liquid fermentation basal medium Culture is to get finished product in middle culture 8-10d.
- 2. the method according to claim 1 for improving Cordyceps militaris production cordycepin, it is characterised in that：The step （3）In adenine and adenosine mass ratio be 1-2:3-5.
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| CN201611058384.8A CN108118076A (en) | 2016-11-27 | 2016-11-27 | A kind of method for improving Cordyceps militaris production cordycepin |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113005163A (en) * | 2021-03-25 | 2021-06-22 | 泸州品创科技有限公司 | Method for producing cordycepin by continuously culturing cordyceps militaris |
| CN114164118A (en) * | 2020-09-11 | 2022-03-11 | 安康市农业科学研究院 | Preparation method of konjak insect-resistant microbial inoculum |
-
2016
- 2016-11-27 CN CN201611058384.8A patent/CN108118076A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114164118A (en) * | 2020-09-11 | 2022-03-11 | 安康市农业科学研究院 | Preparation method of konjak insect-resistant microbial inoculum |
| CN113005163A (en) * | 2021-03-25 | 2021-06-22 | 泸州品创科技有限公司 | Method for producing cordycepin by continuously culturing cordyceps militaris |
| CN113005163B (en) * | 2021-03-25 | 2023-03-14 | 泸州品创科技有限公司 | Method for producing cordycepin by continuously culturing cordyceps militaris |
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Application publication date: 20180605 |